Product Datasheet

Mouse FLT1,VEGFR1 ELISA Kit

Catalog No: #EK5255

Orders: [email protected] Description Support: [email protected]

Product Name Mouse FLT1,VEGFR1 ELISA Kit

Specificity Mouse

Crossing Reactivity There is no detectable cross-reactivity with other relevant .

Immunogen Type NSO,Y23-E759

Other Names Vascular endothelial growth factor 1; VEGFR-1; 2.7.10.1; Embryonic receptor kinase 2; Fms-like

1; FLT-1; Tyrosine- kinase receptor FLT; Flt1; Emrk2, Flt, Vegfr1;

Accession No. P35969

Cell Localization Cell membrane; Autophosphorylation promotesubiquitination and endocytosis..

Application Details

sensitivity:10pg mlDetect Range:156pg ml-10000pg mlsample_type:cell culture supernates and serum.capture_antibody:monoclonal antibody from

ratdetection_antibody:polyclonal antibody from goatgene_name:Flt1protein_name:Vascular endothelial 1gene_full_name:Vascular

endothelial growth factor receptor 1tissue_specificity:sequence_similarities:tmb_incubation:15-20minresearch_category:

Product Description

Sandwich High Sensitivity ELISA for Quantitative Detection of Mouse FLT1,VEGFR1

Background

protein_function: Tyrosine- that acts as a cell-surfacereceptor for VEGFA, VEGFB and PGF, and plays an essential role inthe development of embryonic vasculature, the regulation ofangiogenesis, cell survival, cell migration, macrophage function,chemotaxis, and cancer cell invasion. May play an essential roleas a negative regulator of embryonic angiogenesis by inhibitingexcessive proliferation of endothelial cells. Can promoteendothelial cell proliferation, survival and angiogenesis inadulthood. Its function in promoting cell proliferation seems tobe cell-type specific. Promotes PGF-mediated proliferation ofendothelial cells, and proliferation of some types of cancercells, but does not promote proliferation of normal fibroblasts.Has very high affinity for VEGFA and relatively low protein kinaseactivity; may function as a negative regulator of VEGFA signalingby limiting the amount of free VEGFA and preventing its binding toKDR. Modulates KDR signaling by forming heterodimers with KDR.Ligand binding leads to the activation of several signalingcascades. Activation of PLCG leads to the production of thecellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediatesphosphorylation of PIK3R1, the regulatory subunit ofphosphatidylinositol 3-kinase, leading to the activation ofphosphatidylinositol kinase and the downstream signaling pathway.Mediates activation of MAPK1,ERK2, MAPK3,ERK1 and the MAP kinasesignaling pathway, as well as of the AKT1 signaling pathway.Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL.Promotes phosphorylation of AKT1 and PTK2,FAK1 (By similarity)..Vascular endothelial growth factor receptor 1 is a protein that in humans is encoded by the FLT1 . Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Note: This product is for in vitro research use only and is not intended for use in humans or animals.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2