Polyclonal Antibody to TLE2 - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP06713PU-N Polyclonal Antibody to TLE2 - Aff - Purified

Alternate names: GRG2, TLE2, Transducin-like enhancer protein 2 Quantity: 0.1 mg Concentration: 1.0 mg/ml Background: The Notch signaling pathway controls cellular interactions important for the specification of a variety of fates in both invertebrates and vertebrates. Key players in the Notch pathway are the TLE genes (for transducin-like enhancer of split, also designated ESG for enhancer of split groucho), which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. TLEs associate with chromatin in live cells and specifically with Histone H3, but not with other core histones. Expression of the TLE genes, TLE1, TLE2, TLE3 and TLE4, correlate with immature epithelial cells that are progressing toward a terminally differentiated state, suggesting a role during epithelial differentiation. TLE1, TLE2 and TLE3 have elevated expression in cervial squamous metaplasias and carcinomas, while TLE4 is most highly expressed in the brain, particularly in the caudate nucleus. TLE1 and TLE4 contain SP and WD40 domains, through which TLE1 binds AML1 to inhibit AML1-induced transactivation of the CSF1 receptor. Uniprot ID: Q04725 NCBI: NP_001138233.1 GeneID: 7089 Host: Rabbit Immunogen: Synthetic peptide, corresponding to amino acids 200-250 of Human TLE2. Format: State: Liquid purified Ig fraction Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) Buffer System: Phosphate buffered saline (PBS), pH 7.2. Preservatives: 15 mM sodium azide Applications: Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Molecular Weight: ~ 80 kDa Specificity: This antibody detects endogenous levels of TLE2 protein. (region surrounding Lys214) Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse and Rat.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. VA/20140919 1 / 2 AP06713PU-N: Polyclonal Antibody to TLE2 - Aff - Purified

Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Pictures: Western blot (WB) analysis of TLE2 antibody (Cat.-No.: AP06713PU-N) in extracts from hela cells.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. VA/20140919 2 / 2