Anti- Teplizumab (Anti-Idiotype) Recombinant Monoclonal Rabbit IgG Clone # 2505B Catalog Number: MAB10245

DESCRIPTION Specificity Detects Teplizumab in Western blots and direct ELISAs. In Western blots, no cross-reactivity with , , Satumomab, and is observed. Source Recombinant Monoclonal Rabbit IgG Clone # 2505B Purification Protein A or G purified from cell culture supernatant Immunogen Full-length Teplizumab Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

APPLICATIONS Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website. Recommended Sample Concentration Western Blot 2 µg/mL See Below Flow Cytometry 0.25 µg/106 cells See Below

DATA

Western Blot Flow Cytometry Detection of Teplizumab by Western Detection of Anti-CD3 (Teplizumab) on Human PBMC by flow Blot. Western blot shows Daclizumab cytometry. Human PBMC were stained with (A) Human CD3 (negative control), Basiliximab (negative (Research Grade Teplizumab Biosimilar) control), Satumomab (negative control), (Catalog # MAB9929)or (B) Rabbit IgG control antibody (Catalog # Teplizumab, and Alemtuzumab (negative MAB1050), followed by Rabbit anti-CD3 (Teplizumab) (Anti-Idiotype, control). PVDF membrane was probed with Catalog # MAB10245), then APC-conjugated anti-rabbit IgG 2 µg/mL of Rabbit Anti-Anti- Teplizumab secondary antibody (Catalog # F0111), followed by Mouse anti- (Anti-Idiotype) Monoclonal Antibody (Catalog Human CD3 PE-conjugated Monoclonal Antibody (Catalog # # MAB10245) followed by HRP-conjugated FAB100P). View our protocol for Staining Membrane-associated Anti-Rabbit IgG Secondary Antibody Proteins. (Catalog # HAF008). A specific band was detected for Teplizumab at approximately 250 kDa (as indicated). Human IgG is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

PREPARATION AND STORAGE Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (-SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

BACKGROUND The anti-Teplizumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Teplizumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Rev. 12/26/2019 Page 1 of 1