Applications: Quality Assurance: Proteinase K has been purified RNase Activity: Incubation of 6 units of enzyme free of exonucleases and endonucleases by for 4 hours at 37°C in 50 µl assay buffer Isolation of plasmid and genomic DNA • Finnzymes Oy (Finland), a strategic partner of New with 1 µg MS2 RNA resulted in no detectable Proteinase K • Isolation of RNA England Biolabs. Quality controls are performed degradation of the RNA as determined by gel • Inactivation of RNases, DNases and enzymes at Finnzymes Oy and confirmed at New England electrophoresis. 1-800-632-7799 in reactions Biolabs. [email protected] Reference: www.neb.com P8102S 016120314031 Supplied in: 20 mM Tris-HCl (pH 7.4), 1 mM CaCl2 Quality Control Assays 1. Anson, M.L. (1939) J. Gen. Physiol. 22, 79. and 50% glycerol. 16-Hour Incubation: A 50 µl reaction containing 1 µg of λ DNA/HindIII digest and 1.8 units of enzyme Reaction Conditions: Proteinase K is active in a incubated for 16 hours resulted in the same P8102S wide range of buffers (pH 4.0–12.5) temperatures pattern of DNA bands as a reaction incubated for (37–60°C) and salts such as GuHCl (3 M) and 60 mg Lot: 0161203 Exp: 3/14 one hour. urea (4 M). It is also active in detergents such as 20 mg/ml Store at –20°C Tween 20 (5%), Triton X-100 (1%) and SDS (1%). Exonuclease Activity: Incubation of 6 units of enzyme with 1 µg sonicated 3H DNA (105 cpm/µg) Description: Proteinase K is a non-specific, Unit Definition: One unit is defined as the amount for 4 hours at 37°C in 50 µl reaction buffer subtilisin-related serine protease with a very high of enzyme required to liberate folin-positive amino released < 0.2% radioactivity. specific activity. Proteinase K is not inactivated acids and peptides corresponding to 1 µmol of by chelating reagents such as EDTA or detergents tyrosine in 1 minute at 37°C in a total reaction Endonuclease Activity: Incubation of 1.8 units such as SDS and is active over a wide range of volume of 250 µl (1). of enzyme with 1 µg φX174 RF I DNA for 4 hours pH (4–12.5). at 37°C in a 50 µl reaction buffer resulted in Source: Tritirachium album Specific Activity: 30 units/mg < 5% conversion to RF II.
Molecular Weight: 28.9 kDa
CERTIFICATE OF ANALYSIS
Applications: Quality Assurance: Proteinase K has been purified RNase Activity: Incubation of 6 units of enzyme free of exonucleases and endonucleases by for 4 hours at 37°C in 50 µl assay buffer Isolation of plasmid and genomic DNA • Finnzymes Oy (Finland), a strategic partner of New with 1 µg MS2 RNA resulted in no detectable Proteinase K • Isolation of RNA England Biolabs. Quality controls are performed degradation of the RNA as determined by gel • Inactivation of RNases, DNases and enzymes at Finnzymes Oy and confirmed at New England electrophoresis. 1-800-632-7799 in reactions Biolabs. [email protected] Reference: www.neb.com P8102S 016120314031 Supplied in: 20 mM Tris-HCl (pH 7.4), 1 mM CaCl2 Quality Control Assays 1. Anson, M.L. (1939) J. Gen. Physiol. 22, 79. and 50% glycerol. 16-Hour Incubation: A 50 µl reaction containing 1 µg of λ DNA/HindIII digest and 1.8 units of enzyme Reaction Conditions: Proteinase K is active in a incubated for 16 hours resulted in the same P8102S wide range of buffers (pH 4.0–12.5) temperatures pattern of DNA bands as a reaction incubated for (37–60°C) and salts such as GuHCl (3 M) and 60 mg Lot: 0161203 Exp: 3/14 one hour. urea (4 M). It is also active in detergents such as 20 mg/ml Store at –20°C Tween 20 (5%), Triton X-100 (1%) and SDS (1%). Exonuclease Activity: Incubation of 6 units of enzyme with 1 µg sonicated 3H DNA (105 cpm/ Description: Proteinase K is a non-specific, Unit Definition: One unit is defined as the amount µg) for 4 hours at 37°C in 50 µl reaction buffer subtilisin-related serine protease with a very high of enzyme required to liberate folin-positive amino released < 0.2% radioactivity. specific activity. Proteinase K is not inactivated acids and peptides corresponding to 1 µmol of by chelating reagents such as EDTA or detergents tyrosine in 1 minute at 37°C in a total reaction Endonuclease Activity: Incubation of 1.8 units such as SDS and is active over a wide range of volume of 250 µl (1). of enzyme with 1 µg φX174 RF I DNA for 4 hours pH (4–12.5). at 37°C in a 50 µl reaction buffer resulted in Source: Tritirachium album Specific Activity: 30 units/mg < 5% conversion to RF II.
Molecular Weight: 28.9 kDa
CERTIFICATE OF ANALYSIS