Annals of Medicine

ISSN: 0785-3890 (Print) 1365-2060 (Online) Journal homepage: http://www.tandfonline.com/loi/iann20

Translational Research and Innovation in Human and Health Science

To cite this article: (2018) Translational Research and Innovation in Human and Health Science, Annals of Medicine, 50:sup1, S10-S170, DOI: 10.1080/07853890.2018.1427445 To link to this article: https://doi.org/10.1080/07853890.2018.1427445

Published online: 20 Apr 2018.

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Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=iann20 ANNALS OF MEDICINE, 2018 VOL. 50, NO. S1, S10–S170 https://doi.org/10.1080/07853890.2018.1427445

ABSTRACTS

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BIOLOGY Preliminary characterization of mucins of tagi

Catarina Fernandes, Paulo Mascarenhas and Zilda Morais CiiEM, Egas Moniz Cooperativa de Ensino Superior, Almada, Portugal

Introduction: Osteoarthritis is the third most frequent rheumatic disease in Portugal, affecting 50% of the population aged over 50 years. Current treatments have several adverse effects, which leads to the need to find alternatives. Recent studies by Takagaki et al. [1] on mucin presented encouraging results, in particular by raising the hypothesis of joint cartilage regeneration. Previous research of Masuda et al. [2] showed a similarity between the human mucin type MUC5AC and a jellyfish mucin named quiumucin, in the tandem repeat of peptide chain and also in water solubility. The present project aims to char- acterize the mucins of the Catostylus tagi, native of Tagus and Sado estuaries [3], in order to initiate the studies about its possible use in the treatment of osteoarthritis. Materials and methods: Exemplars of C. tagi were collected at Tagus estuary, brought to Egas Moniz laboratory, homogenised and dialised against water type II in a membrane pore <1 kDa, water changed 3 24h. Desalted sample was lyophilised. A poly- acrylamide gel with 1 mm spacer and 4–12% crosslinking was homemade with reagents and Mini-PROTEANVR Tetra Handcast Systems from BioRad. Sample preparation for electrophoresis included suspension of 4 mg in 1 mL Laemmli buffer/b-mercaptoe- thanol (95/5), heating for 5 min at 95 C, cooling in ice for 10 min and centrifugation at 1000 rpm for 10 min. Application volumes of supernatant were 5, 10, 15 and 20 lL. The applied voltage was 150 V. The gel was stained with Coomassie Brilliant Blue R250. Results: At least 12 bands from 250 to 10 kDa were found in C. tagi sample, the ones near 50, 15 and 75 kDa being the most intense (Figure 1), which is in line with the possible occurrence of quiumucin [2]. The bands near 50 and 75 were selected for PMF analysis at Instituto de Investigac¸ao~ e Inovac¸ao~ em Saude da Universidade do Porto. Discussion and conclusions: The results showed no significant similarity between the sequence found in the C. tagi mucin and the one found in qniumucin. However, the band near 50 had the same five amino acids with similar molecular mass as the one of the mucins found in C. mosaicus [4].

CONTACT Catarina Fernandes [email protected] Acknowledgements This study was funded by Egas Moniz Coop Ensino Superior CRL, project EM-ZM 03/04.

References [1] Takagaki T, Sato M, Kawake T, et al. Interactions between jellyfish mucin and hyaluronan in human chondrocytes. Int J Biol Pharmaceut Res. 2015;6:351–358.

Figure 1. Left: Protein standards. Right: C. tagi whole medusa. Arrows highlight the possible region for a band of qniumucin.

ß 2018 Informa UK Limited, trading as Taylor & Francis Group ANNALS OF MEDICINE S11

[2] Masuda A, Baba T, Dohmae N, et al. Mucin (qniumucin), a glycoprotein from jellyfish, and determination of its main chain structure. J Natural Prod. 2