Transcriptomic Exploration of the Vanessa Cardui Immune System

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University of Nevada, Reno

Transcriptomic Exploration of the Vanessa cardui Immune System

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Ecology, Evolution, and Conservation Biology

Alexander Drake Selvey

Dr. Angela Smilanich/Thesis Advisor

August, 2020

THE GRADUATE SCHOOL

We recommend that the thesis prepared under our supervision by

entitled

be accepted in partial fulfillment of the requirements for the degree of

Advisor

Committee Member

Graduate School Representative

David W. Zeh, Ph.D., Dean Graduate School

ABSTRACT

Vanessa cardui, a polyphagous Nymphalid butterfly known as the painted lady, is an ideal species for understanding ecological interactions influencing immunity, but to date has few molecular resources available to examine the underlying physiological processes driving the regulatory mechanisms of these dynamics. Rapid technological advancements in sequencing technology have decreased sequencing costs to the point of feasibility for studying the molecular basis of ecological models such as V. cardui. To examine gene expression after infection with two unique pathogens, we acquired commercially available V. cardui, infected larvae in the lab with either Escherichia coli or

Junonia coenia Densovirus (JcDNV), then sequenced the transcriptome to establish an immune profile for infected larvae and controls. Gene ontology (GO) and differential expression (DE) analyses pointed to an over-representation of genes associated with developmental and energy utilization pathways with no under-representation noted for any pathways after bacterial infection. For viral infection, there was also an over- representation of genes associated with metabolic and energy usage pathways and an under-representation of genes associated with pathways involved in regulatory processes and gene expression. When comparing viral and bacterial infections, there was an over- representation of genes associated with responses to biotic stimulus, defense responses, biosynthetic responses, and movement of cellular components, with an under- representation of genes associated with cellular processes and metabolic processes.

Closer investigation revealed preexisting viral populations circulating in the commercially acquires specimens, potentially confounding results based on the proposed research design. However, the tools created from this investigation still hold value for ii further investigation into the immune response of V. cardui by providing another valuable transcriptomic resource that can be combined with the few existing, non-immune related, resources; making future investigations into any and all molecular functions more powerful and informative. In summary, this transcriptomic investigation pioneers efforts to bring powerful molecular biology tools to address ecological questions of caterpillar immunity.

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DEDICATION

To my wife for being patient and understanding of my academic obligations while raising our children, making the best of our limited income, and always encouraging me to follow my intellectual instinct, even when it may have complicated my research and affected my overall results. Without your support, I would not have come close to being as successful as I am today, I love you with all my heart. To my children for being the main motivation for me to seek a higher education and show them that their possibilities are limitless if they attack their cause with dedication, enthusiasm, and integrity. Never give up on your dreams, even when faced with the most daunting of adversaries, for our

Father did not give us a spirit of fear, but of power, and of love, and of a sound mind (2

Timothy 1:7).

ACKNOWLEDGMENTS

First and foremost, I would like to extend my utmost gratitude to Dr. Angela

Smilanich, who willingly accepted a stray graduate student struggling to find their proper fit in higher education, into her lab group and gave him purpose. Without your compassion, this work would have never come to fruition, so I owe you much more than a thank you; I owe my future as an intellectual to your kindness. No matter how difficult this project became, throughout all the setbacks and unexpected discoveries, you maintained your positivity and encouraged me to continue making unexpected discoveries in search of the truth. For these things I am not only a better person, but a more well-rounded intellectual and I thank you dearly.

Thank you to Dr. Andrew Nuss for your help in funding this project and for all your support throughout. Your insight and suggestions have been greatly appreciated.

Thank you to Dr. Nuss’ lab, most notably Gurlaz Kaur and Rana Pooraiiouby, for all of your help with and suggestions on analyses throughout the project.

A big thank you to Dr. Richard Tillett and the Nevada Center for Bioinformatics for supporting this project with an INBRE grant from the National Institute of General

Medical Sciences (GM103440). The lessons learned have been invaluable to my growth as a graduate student and amateur bioinformatician. The assistance with best practices for

RNA-seq analysis has been a learning experience that I will not soon forget

I would also like to thank my committee (Dr. Pedro Miura and Dr. Andrew Nuss), for embracing the challenges that I have had and never losing hope that I would finally come to a coherent conclusion based on fact. Also, for assembling rapidly and allowing me to graduate after a major degree path switch, allowing me to pursue a career to v support my growing family. I thank you for your hard work and unrivaled dedication to making this happen on such a short turnaround.

A special thanks to the wonderful graduate students and professors that I grew to know and love in the various classes and laboratories that I was fortunate enough to take and work in. As well as, those professors that allowed me to rotate through their laboratories to earn invaluable skills across a myriad of disciplines and technological specialties, to you I owe a large debt of gratitude and I thank you for the sacrifices you made on my behalf.

TABLE OF CONTENTS INTRODUCTION ...... 1 METHODS ...... 5 Focal Organism ...... 5 Pathogens ...... 5 Treatment Groups ...... 7 Caterpillar Rearing ...... 7 RNA Extraction, Library Preparation, & Sequencing ...... 8 Transcriptome Assembly ...... 8 Transcriptome Annotation & Quality Assessment ...... 9 Differential Expression Analysis ...... 10 Gene Ontology Enrichment Analysis ...... 10 Centrifuge Sequence Classification ...... 10 RESULTS ...... 12 Transcriptome Assembly Quality & Annotation ...... 12 Immune System Components ...... 14 Differential Expression & GO Analyses ...... 14 Profile of Viral Transcripts in V. cardui ...... 17 DISCUSSION ...... 52 CONCLUSION ...... 56 REFERENCES ...... 57 APPENDIX ...... 63 List of Supplementary Tables ...... 63 List of Supplementary Figures ...... 148

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LIST OF TABLES

Table 1. Number of reads before and after trimming ...... 18

Table 2. BUSCO statistics and general transcriptome statistics obtained from EnTAP ....19

Table 3. Predicted set of immunity genes from the Nuss-Smilanich assembled transcriptome based on prior lepidopteran immunity gene annotations ...... 20

Table 4. Kallisto alignment statistics: trimmed reads against Nuss-Smilanich transcriptome (final filtered). (Samples n=12, Transcripts: n=22,342) ...... 38

Table 6. Comparison of all the lepidopteran viruses found in the individual replicates of each treatment (JcDNV in bold)...... 42

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LIST OF FIGURES

Figure 1. PCA comparisons of samples and treatments, with the x-axis being the variance across PC1 and the y-axis the variance across PC2. Treatments are depicted as orange or turquoise, with sample numbers as different shapes. Sample number one is depicted by a circle, sample two by a triangle, and sample three by a square. A) Virally-infected (turquoise) vs. Non-infected (orange); B) Bacterially-injected (orange) vs. saline-injected (turquoise); C) Virally-infected (turquoise) vs. Bacterially-injected (orange); D) Non-infected (orange) vs. saline-injected (turquoise)...... 48

Figure 2. MA plots of the differential expression results plotting log2 fold changes (LFC) on the y-axis against the mean of normalized counts on the x-axis. Shrunken LFC values were generated utilizing the apeglm R package while running DESeq2. Red dots signify differentially expressed transcripts, gray dots signify non-differentially expressed transcripts. A) Virally-infected vs. Non-infected; B) Bacterially-injected vs. saline-injected; C) Virally-infected vs. Bacterially-injected; D) Non-infected vs. saline-injected ...... 49

Figure 3. Volcano plots depicting the significantly differentially expressed transcripts with false detection rates (FDR; padj) of less than 0.05 and a fold change (FC) of greater than 0.5. Blue dots represent transcripts that do not meet either threshold. Green dots represent transcripts that meet the log2 FC threshold, but not the FDR (padj) threshold. Orange dots represent transcripts that meet both the log2 FC and the FDR thresholds. A) Virally-infected vs. Non-infected; B) Bacterially-injected vs. saline-injected; C) Virally-infected vs. Bacterially-injected; D) Non-infected vs. saline-injected ...... 50

Figure 4. Top ten enriched GO IDs (p-value < 0.01) for each comparison and their associated terms for each GO category: biological process (BP), cellular component (CC), and molecular function (MF). The number of differentially expressed genes for each category is displayed across the x-axis. A) Virally-infected vs. Non-infected; B) Bacterially-injected vs. saline-injected; C) Virally-infected vs. Bacterially-injected; D) Non-infected vs. saline-injected ...... 51

INTRODUCTION

As stated in Price et al. (1980), “...insect-plant interactions cannot progress realistically without consideration of the third trophic level,” with the third trophic level being natural enemies of herbivores. The development of the field of ecological immunology, or ecoimmunology, has in part been built upon this premise that, interactions with parasitic enemies has shaped host immunity, but has repercussions on mechanisms governing life-history evolution, sexual selection, and population dynamics

(Sheldon & Verhulst, 1996; Schulenburg et al., 2009). The field of ecoimmunology effectively integrates the third trophic level (natural enemies) into its framework, fulfilling the assertion of Price et al. (1980) and providing valuable insight into pathogen- herbivore-plant interactions. Ecoimmunology consists of three central themes: 1) whole organism immunity, 2) immune heterogeneity, and 3) trade-offs based on variation in individual immunity (Schoenle et al., 2018). Two major contributions to ecoimmunological research were made by undertaking this project. The first deals with the lack of information about the genes related to immune function within non-model systems. The lack of a comprehensive immunity gene list for V. cardui and other non- model Lepidopteran species makes utilizing them as a model-system for immune related studies much more time and computationally intensive. With V. cardui being widely and readily available for laboratory experiments and the fact that this generalist lepidopteran will eat an artificial diet, developing immunological tools for V. cardui would increase the ability of researchers to use it for ecoimmunological studies and expand our understanding of insect immunity. Here, we present a list of immunity related genes and their genetic identities that will allow future researchers to streamline studies of immunity 2 in this lepidopteran and other related species. The second is the specificity of such immunity genes to various pathogens which addresses the question of immune response heterogeneity within individuals. The research presented here has the potential to aid in future studies of pathogen specific transcription of immunity genes by offering a starting point for expression of genes with immune-related functions for bacterial and viral studies. Overall, we provide, not only lists of genes with immune functions, but a plethora of bioinformatic information that may be utilized to improve or combined with existing bioinformatic research to increase the knowledge base surrounding the expression patterns and genetic makeup V. cardui.

The innate immune response in insects is reminiscent of its mammalian counterpart: effector molecules (chemokines, cytokines, pathogen-associated molecular patterns (PAMPs), endogenous ligands, NF-κB, etc.) bind to a constitutively expressed receptor (pattern-recognition receptor (PRR), Toll-like receptors (TLRs), IMD pathway receptors, JNK pathway receptors, peptidoglycan recognition proteins (PGRPs), etc.) in turn activating a cascade of biochemical reactions. The resulting cascade leads to genetic regulation and activation of defense molecules (antimicrobial peptides (AMPs), phenol oxidase (PO), reactive oxygen species (ROS), etc.) (Vilmos & Kurucz, 1998; Zhang et al., 2013; Mikonranta et al., 2014; Iwasaki & Medzhitov, 2015; Cooper & Eleftherianos,

2017). The innate immune response also includes immune cells called hemocytes to combat foreign entities that enter their systems, complementing an extraordinarily complex set of regulatory pathways to eliminate pathogens and increase survival

(Beckage, 2008). Even with a robust and effective innate immune response, insects lack a fully developed adaptive immune response (Cooper & Eleftherianos, 2017). Despite the 3 lack of a highly specific response, the innate insect immune response has much more specificity that previously known (Cooper & Eleftherianos, 2017).

What can be seen as the equivalent to an adaptive immune response in insects is much different than its mammalian counterpart in that it is mostly attributed to what is known as immunological priming of insect hemocytes, but in certain invertebrates can be marginally reminiscent due to alternative splicing of PRRs (Müller et al., 2008; Cooper &

Eleftherianos, 2017). Immunological priming is the process by which the innate insect immune system is exposed to dead microorganisms or a sublethal dose of a live pathogen, eliciting an immune response which leads to a protective effect upon secondary challenge with what may be considered a lethal dose of pathogen (Müller et al., 2008).

Immune priming in the insect adaptive-like immune response is not limited to a single generation and has been shown to provide protective effects trans-generationally in a process known as trans-generational immune priming (TgIP), suggesting an even more developed adaptive-like, priming immune response than what has been traditionally accepted (Moret, 2006). Investigations into such a subject have often been undertaken using reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR), but due to the development of exponentially more affordable and rapid sequencing technologies, more recent studies into the activation/evolution of innate/adaptive immunity in insects have incorporated RNA-Sequencing (RNA-Seq) into their analyses (Zhang et al., 2015;

Wu & Yi, 2018; Yi et al., 2019; Tan et al., 2019; Kutsukake et al., 2019). Zhang et al.

(2015) found that locust fat body and hemocytes adopt different strategies in response to a fungal insect pathogen through differential expression analyses of RNA-Seq data. Wu and Yi (2018) were able to characterize the expression profiles of Bacillus thuringiensis 4

(Bt) infected Bombyx mori larval hemocytes. In Yi et al. (2019) they were able to better characterize the biological and molecular mechanisms regulating the specificity of invertebrates immune priming through utilization of RNA-Seq methodologies. Finally,

Kutsukake et al. (2019) found a link between individual immunity and social immunity utilizing RNA-Seq methods. Nonetheless, transcriptomic characterization of the insect immune response outside of model organisms is still rare, especially in conjunction with an immune challenge from different pathogen taxa.

Here, an RNA-Seq analysis was done to shed light on the immune regulation of the painted lady butterfly (Nymphalidae: Vanessa cardui) in response to a common viral pathogen and a ubiquitous bacterial pathogen, similar to the study undertaken by Pascual et al. (2012) in Spodoptera exigua. Immune genes specific to bacterial and viral infection were sought, but all immune genes identified through our de novo transcriptome analysis were considered. Along with genes related to immunity, all genes that were under any deferentially expressed status, up or down, were reported. Two hypotheses were generated: first, expression profiles of both infection statuses would include an upregulation of genes related to insect immunity; second, the expression profiles of the viral and bacterial groups would differ based on the fundamental differences between the immune reaction to viral and bacterial pathogenic challenges.

METHODS

Focal Organism

The species used in this study were larvae of the nymphalid butterfly, Vanessa cardui. Vanessa cardui along with Vanessa kershawi, Vanessa carye, Vanessa annabella,

Vanessa virginiensis, and occasionally Vanessa altissima make up the Cynthia subgroup

(Otaki, 2007). Vanessa cardui is notable for being one of the most, if not the most, widespread butterfly species in the world and has been referred to as, “cosmopolitan”

(Field, 1971; Ecuador, 1992). Vanessa cardui is a generalist butterfly, feeding on over 100 different plant species, feeding principally on Asteraceae (Compositae) (Field, 1971;

Scott, 1986). The distribution of V. cardui lies primarily within the Northern Hemisphere and extends substantially into the Southern Hemisphere only on the African continent, having an elevation range from sea level to 5200m (Ecuador, 1992). The broodedness of

V. cardui is continuous and without diapause; overwintering occurs along the extreme north African coast, extensively along the western margin of the Red Sea and in Saudi

Arabia, and in the Sonoran and Chihuahuan deserts of North America (Ecuador, 1992).

Pathogens

The viral pathogen used in this study, Junonia coenia densovirus (JcDNV), is a

Parvovirus (Family: Parvoviridae) of the subfamily Densovirinae within the genus

Ambidensovirus first described by Rivers and Longworth (1972). JcDNV is a generalist arthropod virus that primarily infects lepidopterans of various lineages, such as Mamestra brassicae, Bombyx mori, Lymantria dispar (Rivers & Longworth, 1972), and various

Spodotera spp. (Mutuel et al., 2010). JcDNV has a non-enveloped icosahedral virion containing an ambisense 6,032 nucleotide (nt) single-stranded DNA (ss-DNA) genome 6 that is 83 to 87% identical to other viruses in the densovirus genus; having conserved open reading frames (ORFs) across the densovirus genus along with conserved splicing sites with those identified for Galleria mellonella (GmDNV) and Mythimna loreyi

(MlDNV) (Pham et al., 2013). The main route of infection for JcDNV begins with ingestion of viral particles and proceeds by crossing the insect midgut by transcytosis

(Wang et al., 2013). After transcytosis across the midgut, viral particles come into contact with tracheal cells and hemocytes that are permissive to JcDNV replication (Mutuel et al.,

2010) where viral particles enter cells by rapid clathrin-mediated endocytosis at an estimated particle-to-infectivity ratio of 1:100,000 (Vendeville et al., 2009). Productive infections of JcDNV requires late endosomal trafficking, with activation happening through endosomal acidification, followed by trafficking to the nucleus that requires the host to have an intact cytoskeleton (Vendeville et al., 2009). JcDNV infection leads to disruption of epithelial barrier function (Wang et al., 2013), molting distress, tracheal overgrowth, respiratory distress, and eventually death by hypoxia (Mutuel et al., 2010).

JcDNV infection also leads to increased organismal development time and has an immunosuppressive effect (Smilanich et al., 2018). Recent data (Smilanich et al. unpublished data) has confirmed that JcDNV is present in wild populations of V. cardui, being quantified at detectable levels from wild caught adults utilizing qPCR.

The bacteria used for this study was a nonpathogenic E. coli (DH5-α). E. coli is a gram-negative bacterium found ubiquitously in nature and is often used as a nonpathogenic challenge in studies of lepidopteran immunity (Hoffmann et al., 1981;

Eleftherianos et al., 2006; Gandhe et al., 2006; Gandhe et al. 2007; Cheng et al., 2016).

Although E. coli has been identified in the lepidopteran microbiome (Tang et al. 2012), 7 high dosages of nonpathogenic E. coli have been shown to elicit an immune response

(see citations above).

Treatment Groups

Virus inoculated treatment groups and bacteria injected treatment groups were established. The virus treatment groups consisted of the following: a non-infected group

(n=3) and an infected group inoculated at the larval stage (n=3). Larvae were orally infected with a concentration of 1 x 107 JcDNV particles/µl. Leaf discs were punched from Plantago lanceolata (Plantaginaceae) leaf tissue and 1 µl of virus suspension was pipetted onto the leaf surface. Caterpillars were given 24 hrs to consume the viral laden leaf disc. Afterwards, virally-infected caterpillars were fed the same artificial diet the non-infected, bacterially-injected, and saline-injected caterpillars were fed. The bacteria treatment groups consisted of the following: a saline injected group (n=3) and a bacterially injected group (n=3). This resulted in a total sample size of n=12 (n=3 viral, n=3 viral control, n=3 bacterial, n=3 bacterial control). All caterpillars were sacrificed 72 hours post inoculation.

Caterpillar Rearing

V. cardui eggs were purchased from Carolina Biological (N. Carolina, USA). The resulting larvae were randomly assigned to one of the four treatment groups described above. Caterpillars were reared in an incubator (16 hours light:8 hours dark, 25℃ daytime:20℃ nighttime. Percival Scientific) and fed a plant leaf diet of Plantago lanceolata until the 5th and final larval instar was reached, at which point treatments of bacteria or virus were applied. Non-infected control caterpillars and saline injected caterpillars were reared in a laboratory that did not contain any bacterial or viral 8 pathogens. Viral and bacterial infected caterpillars were reared in a separate laboratory where experimental infection projects are conducted. Bacterial and viral groups were kept in separate incubators to prevent cross contamination.

RNA Extraction, Library Preparation, & Sequencing

RNA extraction was performed on whole body caterpillars utilizing the phenol- chloroform method outlined in the Invitrogen TRIzol Reagent user guide (ThermoFisher

Scientific). Total RNA was submitted to GENEWIZ, South Plainfield, NJ, where library preparation was done using Poly(A) selection and purified mRNA was sequenced utilizing an Illumina HiSeq machine.

Transcriptome Assembly

GENEWIZ

GENEWIZ, NJ was responsible for assembly of the transcriptome and the following was their protocol for assembly. Sequence reads were trimmed to remove adapter sequences and poor quality nucleotides at the 3’ end (error rate < 0.05) using

CLC Genomics Server 9.0 (QIAGEN). Reads shorter than 50 nucleotides were discarded for subsequent analyses. De novo assembly was conducted using trimmed reads from all samples with CLC Genomics Server 9.0 (QIAGEN).

Nuss-Smilanich

In preparation for de novo assembly, raw reads were trimmed using Trimmomatic

(v0.39) (Bolger et al., 2014) in order to remove sequencing adapters and low quality reads. Trinity (v2.9.1) (Grabherr et al., 2011) was utilized to assemble a de novo transcriptome. The resulting transcripts were then used for transcriptome filtering, quality assessment, annotation, and differential expression (DE) analysis. 9

Transcriptome Quality Assessment & Annotation

Transcriptome frame selection filtering to obtain the longest open reading frames

(ORFs) and predict the most likely coding regions, while retaining UniRef90

(DIAMOND (v0.9.32) (Buchfink et al., 2015) blastp) and Pfam-A (El-Gebali et al., 2019)

(HMMER (v3.3) (hmmer.org; Eddy, 2011) hmmscan) homology search results, was done using TransDecoder (v5.5.0) (Hass et al. 2013). To remove redundancy in transcripts generated during assembly and TransDecoder ORF prediction, CD-HIT (v4.8.1) (Fu et al., 2012) was run on the predicted ORFs. In order to decide which transcriptome assembly to move forward with a Benchmarking Universal Single-Copy Orthologs

(BUSCO) (v4.0.5) (Simão et al., 2015; Seppey et al., 2019) analysis was run. The filtered assemblies were run against the Lepidoptera OrthoDB (v10.1) (Kriventseva et al., 2019) database (n=5,286) in order to assess the completeness of each.

Annotation was done using the Eukaryotic Non-Model Transcriptome Annotation

Pipeline (EnTAP) (v0.9.2-beta) (Hart et al., 2020) without frame selection or expression filtering. DIAMOND (v0.9.32) was used for alignment during the similarity search stage.

The databases used were: the APD3 antimicrobial peptide database (Wang et al., 2016),

UniRef90 database (Suzek et al., 2015), the UniProtKB/TrEMBL and UniProtKB/Swiss-

Prot databases (UniProt Consortium, 2019), and the entire NCBI NR database

(containing the non-redundant protein sequences from GenPept, Swissprot, PIR, PDF,

PDB, and NCBI RefSeq) (NCBI Resource Coordinators, 2016). For GO annotation, evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG)

(v4.5.1: protein database; v5.0.0: protein DIAMOND fasta) (Huerta-Cepas et al., 2016;

Huerta-Cepas et al., 2019) databases were used during the orthologous group assignment 10 stage of EnTAP. The final transcriptome draft was generated by pulling all EnTAP annotated sequences out of the original CD-HIT input sequences.

Differential Expression Analysis

Raw reads were trimmed using Trimmomatic (v0.39) (Bolger et al., 2014) in order to remove sequencing adapters and low quality reads. Agat (v0.4.0) (Dianat &

Hereñú, 2020) was used to generate a GTF file from the GFF3 file generated by

TransDecoder, which provided transcript information for usage during alignment.

Trimmed reads were then pseudoaligned to our filtered transcriptome to quantify transcript abundance using kallisto (v0.46.2) (Bray et al., 2016). Kallisto transcript abundances were imported into R (v3.6.3) (R Core Team, 2019) using the R package tximport (v1.14.2) (Soneson et al., 2015) and subsequently analyzed for DE using the R package DESeq2 (v1.26.0) (Love et al., 2014). PCA and MA analyses were done utilizing the DESeq2 commands `plotPCA` and `plotMA`, respectively.

Gene Ontology Enrichment Analysis

GO analysis was done utilizing the R package goseq (v1.38.0) (Young et al.,

2010) to obtain over-represented and under-represented GO id categories at a cutoff value of p < 0.05. GO information was extracted for the over-represented and under- represented GO ids utilizing GO.db (v3.10.0) (Carlson, 2019).

Centrifuge DNA Sequence Classification

Classification of sequenced reads to identify any possible viral contamination was done utilizing Centrifuge (v1.0.4) (Kim et al., 2016). A viral reference index was generated through Centrifuge utilizing all complete viral genomes from the RefSeq NCBI database and raw sequencing reads were ran against it. Any alignments greater than 22 11 contiguous nucleotides (default setting) were considered to be a hit. All results were run against the lepidopteran virus database (Mihara et al. 2016) to identify lepidopteran specific viruses in samples.

RESULTS

Transcriptome Assembly Quality & Annotation

Sequences were trimmed prior to assembly and filtering. Table 1 displays the number of sequencing reads pre- and post-trimming, along with the percentage of reads that were kept and the percentage of reads lost during the process. The average number of reads pre-trimming was 32,390,732 and the average number of reads post-trimming was

28,544,567. The average percentage of reads kept for all samples (n=12) was 88.09% and the average percentage of reads lost for all samples (n=12) was 11.91%; with the highest percentage of reads kept coming from sample VcB3 (89.88%) (VcB3 = Vanessa cardui bacterial treatment 3rd replicate) and the highest percentage of reads lost coming from sample VcV2 (16.54%) (VcV2 = V. cardui virus treatment 2nd replicate).

To begin, the GENEWIZ transcriptome started with 46,485 transcripts and after identifying the longest ORFs and predicting the likely coding regions using

TransDecoder, was reduced to 20,276 transcripts. After utilizing CD-HIT to remove redundant transcripts, the final transcript count equaled 18,304. The Nuss-Smilanich Lab

Trinity assembled transcriptome began with 313,108 transcripts and after identifying the longest ORFs and predicting the likely coding regions using TransDecoder, was reduced to 101,636 transcripts. After removing redundant transcripts using CD-HIT, the final transcript count equaled 28,266.

To assess the quality and completeness of each transcriptome, a BUSCO analysis was run and relevant assembly metrics as given by EnTAP were reported. Table 2 shows the BUSCO scores and EnTAP assembly metrics for both the GENEWIZ assembled and

Nuss-Smilanich Lab assembled transcriptomes. The filtering process was equivalent 13 across both assemblies (i.e. same filtering parameters), with the Nuss-Smilanich Lab assembled transcriptome producing superior marks overall. Looking at the completeness of each assembly, the Nuss-Smilanich Lab assembled transcriptome outperformed the

GENEWIZ assembled transcriptome, capturing 95.4% of all complete BUSCOs (84.4% single-copy, 11.0% duplicated) to the GENEWIZ transcriptomes 59.9% complete

BUSCOs (58.3% single-copy, 1.6% duplicated). For this reason, the Nuss-Smilanich Lab assembled transcriptome was utilized for further analyses.

Utilizing the EnTAP pipeline, out of 28,266 transcripts the total number of unique sequences with an alignment during the similarity search was 20,944 (7,322 unique sequences without alignment). The total number of unique sequences with a gene family assignment was 21,938 (6,328 unique sequences without gene family assignment), the total number of unique sequences with at least one GO term was 15,343. Overall, the total number of unique sequences annotated, during either the similarity search or gene family assignment, was 22,342 (79% annotation rate) (5,924 unique sequences not annotated during either the similarity search or gene family assignment). The 22,342 annotated sequences were used for subsequent analyses. A BUSCO analysis was run to identify any changes in transcriptome completeness. The BUSCO analysis showed no change in overall completeness (C:95.4%; 5045), but showed in increase in single-copy orthologs (S:84.8%; 4485; +24), a decrease in duplicate orthologs (D:10.6%; 560; -24), a decrease in fragmented orthologs (F:1.2%; 65; -1), and an increase in missing orthologs

(M:3.4%; 176; +1).

Immune System Components

After annotation, transcripts were analyzed to elucidate genes involved in immune regulation. Table 3 shows a subset of genes that were successfully annotated with a known immunity gene name from the Manduca sexta (Cao et al. 2015, Kanost et al.,

2016) and Danaus plexippus (Tan et al. 2019) immune gene tables, as well as other widely known insect immune genes. Over 1,000 transcripts were identified as having some direct or indirect relation to an immune-related system or regulatory process through either direct annotation or having a GO category associated with an immune process/function (Supplementary Table S1). A total of 357 transcripts were identified as being known immunity genes through direct annotation (Table 3). Antimicrobial peptides were also found within the annotated transcripts and although no final transcripts bore the annotation from the APD3 database, 24 transcripts were identified to indeed be antimicrobial peptides and are shown in Supplementary Table S2.

Differential Expression & GO Analyses

In order to perform DE analysis, trimmed reads were aligned to the transcriptome using kallisto to generate transcript counts. Table 4 shows the kallisto alignment statistics for all samples (n=12) against the Nuss-Smilanich Lab transcriptome assembly. The average fragment length across all samples (n=12) was 247.519 bp, the average number of reads processed was 28,544,567, the average number of reads pseudoaligned was

18,888,248 (66.22%), and the average number of unique sequences aligned was

16,782,665 (58.84%). The highest alignment rate was seen in sample VcS3 (70.16%)

(VcS3 = Vanessa cardui saline injection 3rd replicate), with the lowest seen in sample

VcV3 (63.64%). Figure 1 shows the PCA analysis for each comparison. In Figure 1A and 15

1C, noticeable differences in grouping of treatments is apparent apart from one sample in each that appears to be more similar to the opposite treatment group. In Figure 1B and

1C, differentiation between treatments is more difficult to achieve, with treatment and samples grouping with their opposite counterpart; the only exception here are samples one and two in Figure 1D, which are more similar to the opposite sample instead of their counterparts. These PCAs, although not entirely normal, are not surprising seeing as we had a low number of replications and the insects that we used did not come from isogenic lines. Figure 2 shows the MA plots for apeglm (Zhu et al., 2019) shrunken and unshrunken log2 fold changes (LFC). The MA plots show proper normalization of our data with no apparent issues. Figure 3A-D display volcano plots for each analysis, with cutoff values of 0.5 for fold change (FC) and 0.05 for false detection rate (FDR/padj).

Blue dots represent transcripts that are not significant (NS) in either category, green dots meet the threshold for LFC, and red dots meet the threshold for both LFC and FDR/padj.

Table 5 shows the top five up-regulated and top 5 down-regulated transcripts for each comparison. A full table of all differentially expressed transcripts can be found in

Supplementary Table S3. Overall, there were 15 transcripts significantly differentially expressed (FDR/padj < 0.05) in the bacteria versus saline comparison, 108 transcripts significantly differentially expressed (FDR/padj < 0.05) in the virus versus non-infected comparison, 48 transcripts significantly differentially expressed (FDR/padj < 0.05) in the virus versus bacteria comparison, and 42 transcripts significantly differentially expressed

(FDR/padj < 0.05) in the non-infected versus saline comparison (Supplementary Table

S3). Out of all the significantly differentially expressed transcripts, 13 were identified as having an immunity related function and are denoted with a red star in Table 5 and 16

Supplementary Table S3. Of the 13 transcripts encoding proteins with immune-related functions: one was uncharacterized, 3 were gloverin-like, and the rest were lebocin-4- like, alaserpin-like, a vascular endothelial growth factor receptor, a serine protease inhibitor, a Reelin domain-containing protein, Hemolin, HIPK, Rac1, and Attacin. The uncharacterized and vascular endothelial growth factor receptor protein transcripts were down-regulated in the VcV vs. VcN comparison. All 3 gloverin-like, the lebocin-4-like, the alaserpin-like, the serine protease inhibitor, the Reelin domain-containing, and Attacin protein encoding transcripts were down-regulated in the VcV vs. VcB comparison.

Hemolin was down-regulated in the VcN vs. VcS comparison, HIPK was up-regulated in the VcB vs. VcS comparison, and Rac1 was up-regulated in the VcV vs. VcN comparison.

GO analysis was done using the R package GO seq and results are shown in

Figure 4 and Supplementary Figures S1-S4. In Figure 4, the top 10 enriched GO categories are plotted with their GO IDs, GO terms, and number of DE genes within the category. Supplementary Figures S1-S4 are bubble plots of all the enriched GO categories and their associated GO terms. Overall, there were 238 enriched GO categories and zero depleted GO category in the bacterial versus saline comparison, 289 enriched

GO categories and 18 depleted GO categories in the virus versus non-infected comparison, 158 enriched GO categories and 28 depleted GO categories in the virus versus bacteria comparison, and 139 enriched GO categories and 3 depleted GO categories in the non-infected versus saline comparison.

Profile of Viral Transcripts in V. cardui

Centrifuge results for treatment versus control sample groups and their corresponding lepidopteran viruses are shown in Table 6. For sample groups VcB, VcS,

VcV, and VcN there were 103, 106, 107, and 104 lepidopteran viruses identified within each, respectively (Table 6). Each sample group contained our target virus JcDNV, with all samples from VcB and VcV containing JcDNV, along with VcS1, VcS3, VcN2 and

VcN3; VcN1 and VcS2 were the only samples that did not contain any traces of JcDNV

(Table 6).

Table 3. Predicted set of immunity genes from the Nuss-Smilanich assembled transcriptome based on prior Lepidopteran immunity gene annotations. Predicted Regulatory Pathway Query Sequence Subject Sequence Description GO Seed Ortholog Gene tr|A0A480KSJ4|A0A480KSJ4_P TRINI- tr|A0A480KSJ4|A0A480KSJ4_P IG Bardet-Biedl syndrome 2 Toll Pathway TY_DN13072_c0_g1_i13. 13037.EHJ72871 AOS1 IG protein isoform X1 OS=Sus p2 scrofa OX=9823 PE=4 SV=1 tr|A0A194QEZ3|A0A194QEZ3_ PAPXU SUMO-activating TRINI- tr|A0A194QEZ3|A0A194QEZ3_ 7091.BGIBMGA00275 enzyme subunit 1 OS=Papilio AOS1 TY_DN2425_c0_g1_i1.p1 PAPXU 5-TA xuthus OX=66420 GN=RR46_07806 PE=4 SV=1 tr|A0A194PV73|A0A194PV73_ PAPXU NF-kappa-B inhibitor TRINI- tr|A0A194PV73|A0A194PV73_ 34740.HMEL013379- cactus OS=Papilio xuthus CACT TY_DN3063_c0_g1_i1.p1 PAPXU PA OX=66420 GN=RR46_05013 PE=4 SV=1 TRINI- XP_026496542.1 cactin 34740.HMEL006752- XP_026496542.1 CACTIN TY_DN7819_c0_g1_i3.p1 [Vanessa tameamea] PA TRINI- XP_026496542.1 cactin 34740.HMEL006752- XP_026496542.1 CACTIN TY_DN1983_c0_g1_i2.p1 [Vanessa tameamea] PA TRINI- XP_026496542.1 cactin 34740.HMEL006752- XP_026496542.1 CACTIN TY_DN1983_c0_g1_i5.p1 [Vanessa tameamea] PA RVE52954.1 hypothetical TRINI- RVE52954.1 protein evm_002431 [Chilo 13037.EHJ64422 CACTIN TY_DN2134_c0_g2_i2.p3 suppressalis] Q8N1I8.1 RecName: Full=Putative uncharacterized TRINI- 9646.ENSAMEP000000 Q8N1I8.1 protein encoded by CACTIN- CACTIN TY_DN2134_c0_g2_i1.p2 06044 AS1; AltName: Full=Cactin antisense RNA 1 [Homo sapiens] Q8N1I8.1 RecName: Full=Putative uncharacterized TRINI- 9646.ENSAMEP000000 Q8N1I8.1 protein encoded by CACTIN- CACTIN TY_DN2134_c0_g2_i1.p2 06044 AS1; AltName: Full=Cactin antisense RNA 1 [Homo sapiens] XP_026496521.1 deformed TRINI- epidermal autoregulatory factor 7091.BGIBMGA00201 XP_026496521.1 DEAF1 TY_DN4809_c1_g1_i3.p1 1 isoform X2 [Vanessa 9-TA tameamea]

XP_026496512.1 deformed TRINI- epidermal autoregulatory factor 7091.BGIBMGA00201 XP_026496512.1 DEAF1 TY_DN4809_c1_g1_i5.p1 1 isoform X1 [Vanessa 9-TA tameamea]

tr|A0A212FFE6|A0A212FFE6_ TRINI- tr|A0A212FFE6|A0A212FFE6_ DANPL GATAd OS=Danaus 34740.HMEL010830- TY_DN5982_c0_g1_i19.p GATAD DANPL plexippus plexippus OX=278856 PA 1 GN=KGM_214691 PE=4 SV=1 XP_026489592.1 G protein- TRINI- 7091.BGIBMGA01074 XP_026489592.1 coupled receptor kinase 2 GPRK2 TY_DN5_c16_g1_i1.p1 7-TA isoform X2 [Vanessa tameamea] tr|A0A517BDU4|A0A517BDU4 TRINI- tr|A0A517BDU4|A0A517BDU4 _HELME Kurtz OS=Heliconius TY_DN2759_c0_g1_i10.p 13037.EHJ69141 KRZ _HELME melpomene OX=34740 PE=2 1 SV=1 tr|A0A194Q0H6|A0A194Q0H6_ TRINI- PAPXU ML domain-containing tr|A0A194Q0H6|A0A194Q0H6_ TY_DN15805_c0_g2_i1.p protein OS=Papilio xuthus 13037.EHJ76124 ML PAPXU 1 OX=66420 GN=RR46_10400 PE=4 SV=1 UniRef90_A0A2G9GNH4 ML TRINI- domain-containing protein n=1 TY_DN91655_c0_g1_i1.p UniRef90_A0A2G9GNH4 Tax=Handroanthus impetigi- 4155.Migut.J00746.1.p ML 1 nosus TaxID=429701 RepID=A0A2G9GNH4_9LAMI XP_026501465.1 myeloid TRINI- differentiation primary response XP_026501465.1 13037.EHJ73995 MYD88 TY_DN1300_c0_g1_i1.p1 protein MyD88 [Vanessa tameamea] TRINI- XP_026489613.1 protein pellino TY_DN2317_c0_g1_i11.p XP_026489613.1 7070.TC009672-PA PLI [Vanessa tameamea] 1 XP_026492855.1 uncharacter- TRINI- XP_026492855.1 ized protein LOC113398352 13037.EHJ70909 SPZ TY_DN8211_c0_g1_i1.p1 [Vanessa tameamea] XP_026492853.1 protein spaetz- TRINI- XP_026492853.1 le-like isoform X3 [Vanessa 13037.EHJ70909 SPZ TY_DN2802_c0_g1_i2.p1 tameamea] TRINI- XP_026492750.1 uncharacter- TY_DN32143_c0_g1_i2.p XP_026492750.1 ized protein LOC113398306 13037.EHJ70909 SPZ 1 [Vanessa tameamea] 21

XP_026492851.1 protein spaetz- TRINI- XP_026492851.1 le-like isoform X1 [Vanessa 13037.EHJ70909 SPZ TY_DN2802_c0_g1_i1.p1 tameamea] TRINI- XP_026495604.1 protein spaetz- TY_DN18300_c0_g1_i4.p XP_026495604.1 13037.EHJ76024 SPZ3 le 3 [Vanessa tameamea] 1 tr|A0A2W1BUZ9|A0A2W1BUZ 9_HELAM Spaetzle domain- TRINI- tr|A0A2W1BUZ9|A0A2W1BUZ containing protein TY_DN16774_c0_g1_i2.p 13037.EHJ74226 SPZ4 9_HELAM OS=Helicoverpa armigera 1 OX=29058 GN=HaOG203363 PE=4 SV=1 tr|A0A5E4PRK1|A0A5E4PRK1 _9NEOP Spaetzle domain- TRINI- tr|A0A5E4PRK1|A0A5E4PRK1 containing protein OS=Leptidea TY_DN13265_c0_g1_i6.p 13037.EHJ75513 SPZ5 _9NEOP sinapis OX=189913 1 GN=LSINAPIS_LOCUS1889 PE=4 SV=1 TRINI- KPJ02437.1 Protein toll [Papilio 7091.BGIBMGA00624 TY_DN21043_c0_g1_i2.p KPJ02437.1 TL xuthus] 4-TA 1 TRINI- XP_026485561.1 protein toll- 7091.BGIBMGA00624 TY_DN19489_c0_g1_i1.p XP_026485561.1 like isoform X2 [Vanessa TL 4-TA 1 tameamea] tr|A0A212F0F7|A0A212F0F7_D TRINI- tr|A0A212F0F7|A0A212F0F7_D ANPL Toll receptor OS=Danaus 34740.HMEL010927- TL TY_DN7787_c0_g1_i1.p1 ANPL plexippus plexippus OX=278856 PA GN=KGM_211472 PE=4 SV=1

TRINI- XP_023936959.1 toll-like 34740.HMEL006338- XP_023936959.1 TLR6 TY_DN1978_c4_g1_i4.p1 receptor 6 [Bicyclus anynana] PA

TRINI- XP_026490649.1 toll-like 34740.HMEL016131- XP_026490649.1 TLR7 TY_DN6141_c0_g3_i1.p1 receptor 7 [Vanessa tameamea] PA

TRINI- XP_021193311.1 protein toll- 34740.HMEL010927- XP_021193311.1 TOLL TY_DN7787_c0_g1_i2.p1 like [Helicoverpa armigera] PA

TRINI- XP_026500902.1 protein toll- XP_026500902.1 13037.EHJ63304 TOLL TY_DN2849_c2_g1_i1.p1 like, partial [Vanessa tameamea] tr|A0A212EH66|A0A212EH66_ DANPL Putative toll TRINI- tr|A0A212EH66|A0A212EH66_ OS=Danaus plexippus plexippus 13037.EHJ63304 TOLL TY_DN46_c0_g1_i4.p1 DANPL OX=278856 GN=KGM_200002 PE=4 SV=1 tr|A0A194Q8L2|A0A194Q8L2_ TRINI- tr|A0A194Q8L2|A0A194Q8L2_ PAPXU Toll-like receptor 4 13037.EHJ74227 TOLL4 TY_DN7978_c0_g1_i2.p1 PAPXU OS=Papilio xuthus OX=66420 GN=RR46_04719 PE=4 SV=1

tr|A0A194RAV9|A0A194RAV9 TRINI- tr|A0A194RAV9|A0A194RAV9 _PAPMA Protein toll 7091.BGIBMGA01108 TY_DN12174_c0_g1_i1.p TOLL6 _PAPMA OS=Papilio machaon OX=76193 4-TA 1 GN=RR48_13491 PE=4 SV=1

tr|A0A346RAG2|A0A346RAG2 TRINI- tr|A0A346RAG2|A0A346RAG2 _9NEOP Toll protein 8 7091.BGIBMGA01108 TOLL8 TY_DN8588_c0_g1_i1.p1 _9NEOP OS=Leguminivora glycinivorella 5-TA OX=1035111 PE=2 SV=1 XP_026492824.1 interleukin-1 TRINI- XP_026492824.1 receptor-associated kinase 4-like 13037.EHJ70951 TUBE TY_DN7851_c0_g1_i1.p1 [Vanessa tameamea]

tr|A0A212EK97|A0A212EK97_ DANPL SUMO-activating TRINI- tr|A0A212EK97|A0A212EK97_ enzyme subunit OS=Danaus 13037.EHJ64540 UBA2 TY_DN1938_c0_g1_i1.p1 DANPL plexippus plexippus OX=278856 GN=KGM_214809 PE=3 SV=1

TRINI- XP_026737876.1 cullin-3 34740.HMEL011639- XP_026737876.1 CUL-3 TY_DN2261_c0_g2_i8.p1 isoform X1 [Trichoplusia ni] PA TRINI- XP_026498611.1 cullin-5 34740.HMEL006498- TY_DN11989_c0_g1_i1.p XP_026498611.1 CUL-5 [Vanessa tameamea] PA 1 22

XP_026488064.1 ubiquitin TRINI- carboxyl-terminal hydrolase 34740.HMEL017223- XP_026488064.1 CYLD TY_DN6723_c1_g3_i2.p1 CYLD isoform X1 [Vanessa PA tameamea] tr|A0A437BNY3|A0A437BNY3 _CHISP Defective in cullin TRINI- tr|A0A437BNY3|A0A437BNY3 34740.HMEL007416- neddylation protein OS=Chilo DCN TY_DN3827_c0_g1_i1.p2 _CHISP PA suppressalis OX=168631 GN=evm_003360 PE=4 SV=1

tr|A0A212EKL9|A0A212EKL9_ DANPL Defective in cullin TRINI- tr|A0A212EKL9|A0A212EKL9_ 34740.HMEL005396- neddylation protein OS=Danaus DCN TY_DN6626_c0_g1_i1.p1 DANPL PA plexippus plexippus OX=278856 GN=KGM_204340 PE=4 SV=1

tr|A0A2H1W4G8|A0A2H1W4G 8_SPOFR Defective in cullin TRINI- tr|A0A2H1W4G8|A0A2H1W4G neddylation protein (Fragment) 34740.HMEL010069- TY_DN4829_c0_g1_i10.p DCN 8_SPOFR OS=Spodoptera frugiperda PA 1 OX=7108 GN=SFRICE_005752 PE=4 SV=1 tr|A0A1E1WMR8|A0A1E1WM R8_PECGO Defective in cullin TRINI- tr|A0A1E1WMR8|A0A1E1WM neddylation protein (Fragment) 34740.HMEL010069- DCN TY_DN4829_c0_g1_i1.p1 R8_PECGO OS=Pectinophora gossypiella PA OX=13191 GN=g.17526 PE=4 SV=1 XP_011556426.1 PREDICTED: TRINI- E3 ubiquitin-protein ligase 34740.HMEL017980- XP_011556426.1 DNR1 TY_DN830_c5_g1_i1.p1 MYLIP-like, partial [Plutella PA xylostella]

tr|A0A212F4X8|A0A212F4X8_ TRINI- DANPL E3 ubiquitin-protein tr|A0A212F4X8|A0A212F4X8_ 34740.HMEL017980- TY_DN11645_c0_g2_i12. ligase MYLIP OS=Danaus DNR1 DANPL PA p1 plexippus plexippus OX=278856 GN=KGM_201119 PE=4 SV=1

TRINI- XP_026494818.1 caspase-8 XP_026494818.1 13037.EHJ75352 DREDD TY_DN4455_c0_g2_i1.p1 [Vanessa tameamea]

tr|A0A194RQQ0|A0A194RQQ0 TRINI- _PAPMA Baculoviral IAP tr|A0A194RQQ0|A0A194RQQ0 7091.BGIBMGA01152 TY_DN1514_c0_g1_i11.p repeat-containing protein 3 IAP2 _PAPMA 3-TA 1 OS=Papilio machaon OX=76193 GN=RR48_07411 PE=4 SV=1

tr|I3V9Q7|I3V9Q7_VANCA TRINI- IMD-like protein OS=Vanessa TY_DN24496_c0_g2_i1.p tr|I3V9Q7|I3V9Q7_VANCA 13037.EHJ71355 IMD cardui OX=171605 GN=imd 1 PE=2 SV=1

tr|S4PLN0|S4PLN0_9NEOP TRINI- NTF2-like export factor 1 7091.BGIBMGA00493 TY_DN13829_c0_g1_i1.p tr|S4PLN0|S4PLN0_9NEOP NXT1 OS=Pararge aegeria OX=116150 3-TA 2 PE=4 SV=1 XP_022130726.1 peptidoglycan TRINI- 34740.HMEL006763- XP_022130726.1 recognition protein-like [Pieris PGRP-SA TY_DN966_c0_g1_i4.p1 PA rapae] XP_026500259.1 peptidoglycan TRINI- 7091.BGIBMGA00058 XP_026500259.1 recognition protein-like isoform PGRP-SA TY_DN158_c0_g3_i1.p1 6-TA X1 [Vanessa tameamea] XP_026500259.1 peptidoglycan TRINI- 7091.BGIBMGA00058 XP_026500259.1 recognition protein-like isoform PGRP-SA TY_DN2587_c1_g2_i2.p1 6-TA X1 [Vanessa tameamea] XP_022130726.1 peptidoglycan TRINI- 34740.HMEL006763- XP_022130726.1 recognition protein-like [Pieris PGRP-SA TY_DN1760_c0_g1_i2.p1 PA rapae] tr|A0A437BHZ9|A0A437BHZ9_ CHISP NTF2 domain-containing TRINI- tr|A0A437BHZ9|A0A437BHZ9_ protein OS=Chilo suppressalis 13037.EHJ63410 RIN TY_DN7090_c2_g1_i1.p2 CHISP OX=168631 GN=evm_005247 PE=4 SV=1 TRINI- XP_026484381.1 protein sickie XP_026484381.1 13037.EHJ78388 SICK TY_DN3461_c0_g1_i5.p1 isoform X6 [Vanessa tameamea]

TRINI- XP_026484380.1 protein sickie TY_DN3031_c0_g1_i10.p XP_026484380.1 13037.EHJ78388 SICK isoform X5 [Vanessa tameamea] 1 TRINI- XP_028042269.1 protein sickie- 34740.HMEL015696- TY_DN85452_c0_g1_i1.p XP_028042269.1 SICK like, partial [Bombyx mandarina] PA 1 tr|A0A212FKS5|A0A212FKS5_ TRINI- tr|A0A212FKS5|A0A212FKS5_ DANPL Sickie OS=Danaus 13037.EHJ78388 SICK TY_DN3461_c0_g1_i6.p1 DANPL plexippus plexippus OX=278856 GN=KGM_204491 PE=4 SV=1 23

tr|A0A212EMA5|A0A212EMA5 _DANPL S-phase kinase- TRINI- tr|A0A212EMA5|A0A212EMA5 associated protein OS=Danaus 13037.EHJ65429 SKPA TY_DN735_c0_g1_i1.p1 _DANPL plexippus plexippus OX=278856 GN=KGM_211966 PE=3 SV=1

tr|B0LD01|B0LD01_DANPL TRINI- Slimb OS=Danaus plexippus tr|B0LD01|B0LD01_DANPL 13037.EHJ76912 SLMB TY_DN1834_c0_g1_i1.p1 OX=13037 GN=slimb PE=2 SV=1

tr|A0A212FBJ8|A0A212FBJ8_D ANPL TAK1-associated binding TRINI- tr|A0A212FBJ8|A0A212FBJ8_D protein 2 isoform A OS=Danaus 13037.EHJ74734 TAB2 TY_DN4137_c0_g1_i5.p1 ANPL plexippus plexippus OX=278856 GN=KGM_200977 PE=4 SV=1

tr|A0A212FBJ8|A0A212FBJ8_D ANPL TAK1-associated binding TRINI- tr|A0A212FBJ8|A0A212FBJ8_D protein 2 isoform A OS=Danaus 13037.EHJ74734 TAB2 TY_DN4137_c0_g1_i1.p1 ANPL plexippus plexippus OX=278856 GN=KGM_200977 PE=4 SV=1

tr|A0A2A4K9Y7|A0A2A4K9Y7 _HELVI Protein kinase domain- TRINI- tr|A0A2A4K9Y7|A0A2A4K9Y7 containing protein OS=Heliothis 13037.EHJ75547 TAK1 TY_DN1114_c0_g2_i5.p1 _HELVI virescens OX=7102 GN=B5V51_37 PE=4 SV=1 tr|A0A2A4K9Y7|A0A2A4K9Y7 TRINI- _HELVI Protein kinase domain- tr|A0A2A4K9Y7|A0A2A4K9Y7 TY_DN1114_c0_g2_i13.p containing protein OS=Heliothis 13037.EHJ75547 TAK1 _HELVI 1 virescens OX=7102 GN=B5V51_37 PE=4 SV=1

tr|A0A212FG39|A0A212FG39_ TRINI- DANPL Ubiquitin conjugating tr|A0A212FG39|A0A212FG39_ TY_DN12452_c0_g1_i1.p enzyme E2 OS=Danaus plexip- 13037.EHJ76453 UBC13 DANPL 1 pus plexippus OX=278856 GN=KGM_201405 PE=3 SV=1

tr|A0A437ASN0|A0A437ASN0 _CHISP UBIQUI- TRINI- tr|A0A437ASN0|A0A437ASN0 TIN_CONJUGAT_2 domain- 13037.EHJ71918 UEV1A TY_DN5161_c0_g1_i1.p1 _CHISP containing protein OS=Chilo suppressalis OX=168631 GN=evm_014241 PE=4 SV=1 TRINI- XP_026501764.1 ALK tyrosine MAPK/JNK/p38/Hippo TY_DN22558_c0_g1_i17. XP_026501764.1 kinase receptor isoform X1 13037.EHJ73692 ALK Pathways p1 [Vanessa tameamea] tr|A0A194Q8R3|A0A194Q8R3_ PAPXU Ets DNA-binding TRINI- tr|A0A194Q8R3|A0A194Q8R3_ 34740.HMEL016694- protein pokkuri OS=Papilio AOP TY_DN1453_c4_g1_i2.p1 PAPXU PA xuthus OX=66420 GN=RR46_05127 PE=3 SV=1 tr|A0A2H1WKE4|A0A2H1WKE TRINI- 4_SPOFR SFRICE_007575 tr|A0A2H1WKE4|A0A2H1WKE 7091.BGIBMGA01229 TY_DN14003_c0_g1_i1.p OS=Spodoptera frugiperda CDC42 4_SPOFR 5-TA 1 OX=7108 GN=SFRICE_007575 PE=4 SV=1 TRINI- XP_026489954.1 activated 34740.HMEL012077- TY_DN6904_c0_g1_i17.p XP_026489954.1 Cdc42 kinase-like isoform X1 CDC42 PA 1 [Vanessa tameamea] TRINI- XP_026489981.1 activated 34740.HMEL012077- TY_DN6904_c0_g1_i16.p XP_026489981.1 Cdc42 kinase-like isoform X4 CDC42 PA 1 [Vanessa tameamea] TRINI- XP_026489970.1 activated 34740.HMEL012077- TY_DN6904_c0_g1_i10.p XP_026489970.1 Cdc42 kinase-like isoform X3 CDC42 PA 1 [Vanessa tameamea] TRINI- XP_026489954.1 activated 34740.HMEL012077- TY_DN6904_c0_g2_i28.p XP_026489954.1 Cdc42 kinase-like isoform X1 CDC42 PA 1 [Vanessa tameamea] tr|A0A1B0YTK3|A0A1B0YTK3 TRINI- tr|A0A1B0YTK3|A0A1B0YTK3 _CHISP ERK1 OS=Chilo 7091.BGIBMGA00145 DSOR1 TY_DN954_c0_g1_i2.p1 _CHISP suppressalis OX=168631 PE=2 8-TA SV=1 tr|A0A212EP52|A0A212EP52_ DANPL F-box/WD repeat- TRINI- tr|A0A212EP52|A0A212EP52_ containing protein TBL1X 34740.HMEL015146- EBI TY_DN1429_c0_g2_i4.p1 DANPL OS=Danaus plexippus plexippus PA OX=278856 GN=KGM_204225 PE=4 SV=1 tr|A0A212EP52|A0A212EP52_ DANPL F-box/WD repeat- TRINI- tr|A0A212EP52|A0A212EP52_ containing protein TBL1X 34740.HMEL015146- TY_DN1429_c0_g1_i10.p EBI DANPL OS=Danaus plexippus plexippus PA 1 OX=278856 GN=KGM_204225 PE=4 SV=1 24

XP_018334882.1 evolutionarily TRINI- conserved signaling intermediate XP_018334882.1 13037.EHJ72448 ECSIT TY_DN3083_c0_g1_i5.p2 in Toll pathway, mitochondrial isoform X2 [Agrilus planipennis]

XP_026488292.1 evolutionarily TRINI- conserved signaling intermediate 34740.HMEL008262- XP_026488292.1 ECSIT TY_DN3083_c0_g1_i2.p1 in Toll pathway, mitochondrial PA [Vanessa tameamea] TRINI- XP_026486587.1 protein eiger TY_DN14702_c0_g1_i2.p XP_026486587.1 13037.EHJ68630 EGR [Vanessa tameamea] 1 tr|A0A1A8DQZ9|A0A1A8DQZ 9_9TELE CDC42 binding TRINI- protein kinase alpha (DMPK- tr|A0A1A8DQZ9|A0A1A8DQZ 34740.HMEL015661- TY_DN15342_c0_g1_i10. like) (Fragment) GEK 9_9TELE PB p1 OS=Nothobranchius kadleci OX=1051664 GN=CDC42BPA PE=4 SV=1 tr|A0A194QBF4|A0A194QBF4_ PAPXU Membrane-associated TRINI- tr|A0A194QBF4|A0A194QBF4_ 34740.HMEL022558- protein Hem OS=Papilio xuthus HEM TY_DN3761_c0_g1_i2.p1 PAPXU PA OX=66420 GN=RR46_02800 PE=4 SV=1 XP_023945335.1 LOW QUAL- TRINI- ITY PROTEIN: homeodomain- 34740.HMEL003132- TY_DN6613_c1_g1_i15.p XP_023945335.1 HIPK interacting protein kinase 2 PA 2 [Bicyclus anynana]

XP_023945335.1 LOW QUAL- TRINI- ITY PROTEIN: homeodomain- 34740.HMEL003132- XP_023945335.1 HIPK TY_DN2939_c1_g1_i6.p1 interacting protein kinase 2 PA [Bicyclus anynana]

XP_023945335.1 LOW QUAL- TRINI- ITY PROTEIN: homeodomain- 34740.HMEL003132- XP_023945335.1 HIPK TY_DN2939_c1_g1_i1.p1 interacting protein kinase 2 PA [Bicyclus anynana]

XP_023945335.1 LOW QUAL- TRINI- ITY PROTEIN: homeodomain- 34740.HMEL003132- XP_023945335.1 HIPK TY_DN2939_c1_g2_i1.p1 interacting protein kinase 2 PA [Bicyclus anynana]

XP_023945335.1 LOW QUAL- TRINI- ITY PROTEIN: homeodomain- 34740.HMEL003132- XP_023945335.1 HIPK TY_DN2939_c1_g2_i7.p1 interacting protein kinase 2 PA [Bicyclus anynana]

XP_023945335.1 LOW QUAL- TRINI- ITY PROTEIN: homeodomain- 34740.HMEL003132- XP_023945335.1 HIPK TY_DN2939_c1_g2_i8.p1 interacting protein kinase 2 PA [Bicyclus anynana] tr|A0A3S2LQ81|A0A3S2LQ81_ CHISP Protein kinase domain- TRINI- tr|A0A3S2LQ81|A0A3S2LQ81_ containing protein (Fragment) 13037.EHJ76637 HIPK TY_DN3012_c3_g1_i2.p1 CHISP OS=Chilo suppressalis OX=168631 GN=evm_013211 PE=4 SV=1 tr|I4DNB1|I4DNB1_PAPXU TRINI- Jun-related antigen OS=Papilio 34740.HMEL011777- tr|I4DNB1|I4DNB1_PAPXU JRA TY_DN9924_c0_g1_i1.p1 xuthus OX=66420 PA GN=RR46_05794 PE=2 SV=1 TRINI- XP_026485623.1 ankyrin repeat TY_DN1288_c1_g1_i41.p XP_026485623.1 domain-containing protein 17 13037.EHJ73802 MASK 1 [Vanessa tameamea] TRINI- XP_026485623.1 ankyrin repeat TY_DN1288_c1_g1_i25.p XP_026485623.1 domain-containing protein 17 13037.EHJ73802 MASK 1 [Vanessa tameamea] TRINI- XP_026485623.1 ankyrin repeat TY_DN1288_c1_g1_i28.p XP_026485623.1 domain-containing protein 17 13037.EHJ73802 MASK 1 [Vanessa tameamea] KPJ13180.1 Ankyrin repeat TRINI- KPJ13180.1 domain-containing protein 17 13037.EHJ73802 MASK TY_DN3295_c1_g1_i4.p1 [Papilio machaon] TRINI- XP_026485623.1 ankyrin repeat TY_DN1288_c1_g1_i33.p XP_026485623.1 domain-containing protein 17 13037.EHJ73802 MASK 1 [Vanessa tameamea] KPJ13180.1 Ankyrin repeat TRINI- KPJ13180.1 domain-containing protein 17 13037.EHJ73802 MASK TY_DN3295_c1_g1_i3.p1 [Papilio machaon] 25

tr|A0A212FGP7|A0A212FGP7_ DANPL Mitogen-activated TRINI- tr|A0A212FGP7|A0A212FGP7_ protein kinase kinase kinase 4 13037.EHJ76732 MEKK1 TY_DN4957_c0_g1_i2.p1 DANPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_213875 PE=4 SV=1 KPI99555.1 Mitogen-activated TRINI- KPI99555.1 protein kinase kinase kinase 4 13037.EHJ76732 MEKK1 TY_DN4957_c2_g1_i5.p1 [Papilio xuthus] tr|A0A212FGP7|A0A212FGP7_ DANPL Mitogen-activated TRINI- tr|A0A212FGP7|A0A212FGP7_ protein kinase kinase kinase 4 TY_DN4957_c0_g1_i10.p 13037.EHJ76732 MEKK1 DANPL OS=Danaus plexippus plexippus 1 OX=278856 GN=KGM_213875 PE=4 SV=1 tr|A0A212EZX7|A0A212EZX7_ DANPL Dual specificity mito- TRINI- tr|A0A212EZX7|A0A212EZX7_ gen-activated protein kinase 13037.EHJ70363 MKK4 TY_DN6712_c0_g1_i5.p2 DANPL kinase 4 OS=Danaus plexippus plexippus OX=278856 GN=KGM_200381 PE=3 SV=1 tr|A0A212F1I3|A0A212F1I3_D TRINI- ANPL NIMA-related kinase 8 tr|A0A212F1I3|A0A212F1I3_D TY_DN35446_c6_g1_i1.p OS=Danaus plexippus plexippus 13037.EHJ70997 NEK8 ANPL 1 OX=278856 GN=KGM_205758 PE=3 SV=1 XP_026494409.1 ETS-like TRINI- 34740.HMEL004734- XP_026494409.1 protein pointed isoform X1 PNT TY_DN3907_c0_g1_i5.p1 PA [Vanessa tameamea] tr|S4PBQ2|S4PBQ2_9NEOP TRINI- 34740.HMEL007742- tr|S4PBQ2|S4PBQ2_9NEOP Rac1 OS=Pararge aegeria RAC1 TY_DN3012_c2_g1_i1.p1 PA OX=116150 PE=4 SV=1 tr|S4PBQ2|S4PBQ2_9NEOP TRINI- 34740.HMEL007742- tr|S4PBQ2|S4PBQ2_9NEOP Rac1 OS=Pararge aegeria RAC1 TY_DN3012_c2_g1_i4.p1 PA OX=116150 PE=4 SV=1 XP_026487083.1 cytoplasmic TRINI- XP_026487083.1 FMR1-interacting protein 13037.EHJ75759 SRA-1 TY_DN3759_c0_g1_i6.p1 [Vanessa tameamea] tr|A0A2A4JF04|A0A2A4JF04_ TRINI- HELVI TRAF-type domain- tr|A0A2A4JF04|A0A2A4JF04_ 34740.HMEL009611- TY_DN26906_c0_g1_i13. containing protein OS=Heliothis TRAF HELVI PA p1 virescens OX=7102 GN=B5V51_3459 PE=4 SV=1

tr|A0A212ETQ8|A0A212ETQ8_ TRINI- DANPL TRAF3-interacting tr|A0A212ETQ8|A0A212ETQ8_ TY_DN15034_c0_g1_i2.p protein 1 OS=Danaus plexippus 13037.EHJ67958 TRAF3IP1 DANPL 1 plexippus OX=278856 GN=KGM_202322 PE=4 SV=1

XP_026491540.1 tumor necrosis TRINI- factor receptor superfamily 34740.HMEL007101- TY_DN100005_c0_g1_i1. XP_026491540.1 WGN member wengen [Vanessa PA p1 tameamea] TRINI- tr|S4P948|S4P948_9NEOP JAK-STAT Pathway TY_DN63732_c0_g1_i1.p tr|S4P948|S4P948_9NEOP Bicaudal C OS=Pararge aegeria 13037.EHJ78372 BICC 1 OX=116150 PE=4 SV=1 TRINI- XP_031764774.1 protein bi- 34740.HMEL016019- TY_DN34130_c0_g1_i1.p XP_031764774.1 caudal C-like [Galleria mellonel- BICC1 PA 1 la] tr|A0A0L7LIV9|A0A0L7LIV9_ 9NEOP Protein bicaudal D TRINI- tr|A0A0L7LIV9|A0A0L7LIV9_ OS=Operophtera brumata 34740.HMEL013410- BICD TY_DN7658_c0_g1_i7.p3 9NEOP OX=104452 PA GN=OBRU01_04945 PE=4 SV=1 tr|A0A194QVL5|A0A194QVL5 TRINI- tr|A0A194QVL5|A0A194QVL5 _PAPMA Protein bicaudal D 34740.HMEL013410- BICD TY_DN3865_c1_g1_i3.p1 _PAPMA OS=Papilio machaon OX=76193 PA GN=RR48_13212 PE=4 SV=1 sp|Q8SWR2|BICRD_DROME TRINI- Bicaudal D-related protein 7091.BGIBMGA01322 TY_DN2035_c4_g1_i11.p sp|Q8SWR2|BICRD_DROME homolog OS=Drosophila mela- BICD 8-TA 1 nogaster OX=7227 GN=CG17365 PE=2 SV=1 XP_026488899.1 bicaudal D- TRINI- 7091.BGIBMGA01322 XP_026488899.1 related protein homolog BICD TY_DN128_c2_g1_i1.p1 8-TA [Vanessa tameamea] TRINI- tr|S4PB13|S4PB13_9NEOP 34740.HMEL016680- TY_DN17036_c0_g1_i5.p tr|S4PB13|S4PB13_9NEOP Caudal OS=Pararge aegeria CAD PA 1 OX=116150 PE=4 SV=1 26

tr|A0A212ERI4|A0A212ERI4_D ANPL Caudal like protein TRINI- tr|A0A212ERI4|A0A212ERI4_D (Fragment) OS=Danaus plexip- TY_DN17036_c0_g1_i2.p 13037.EHJ66999 CAD ANPL pus plexippus OX=278856 2 GN=KGM_215988A PE=4 SV=1 TRINI- XP_026493979.1 tyrosine- TY_DN18895_c0_g1_i2.p XP_026493979.1 protein kinase hopscotch 13037.EHJ65487 HOP 1 [Vanessa tameamea] tr|A0A0N0P9P6|A0A0N0P9P6_ TRINI- PAPXU Ankyrin repeat and tr|A0A0N0P9P6|A0A0N0P9P6_ 34740.HMEL010032- TY_DN22828_c0_g1_i4.p SOCS box protein 17 ASB17 PAPXU PA 2 OS=Papilio xuthus OX=66420 GN=RR46_01473 PE=4 SV=1

tr|A0A212FGD1|A0A212FGD1 TRINI- _DANPL Ankyrin repeat and tr|A0A212FGD1|A0A212FGD1 34740.HMEL008842- TY_DN1661_c0_g1_i10.p SOCS box protein 3 OS=Danaus ASB3 _DANPL PA 1 plexippus plexippus OX=278856 GN=KGM_206607 PE=4 SV=1

XP_026488152.1 SPRY domain- TRINI- containing SOCS box protein 3- 34740.HMEL012348- TY_DN10601_c0_g1_i4.p XP_026488152.1 SPSB3 like isoform X2 [Vanessa PA 1 tameamea] XP_026489286.1 signal trans- TRINI- XP_026489286.1 ducing adapter molecule 1, 13037.EHJ69303 STAM TY_DN9137_c1_g1_i1.p1 partial [Vanessa tameamea] TRINI- XP_022124024.1 signal trans- TY_DN17046_c0_g5_i2.p XP_022124024.1 ducing adapter molecule 1 13037.EHJ69304 STAM 2 [Pieris rapae] tr|A0A5E4R483|A0A5E4R483_ 9NEOP VHS domain-containing TRINI- tr|A0A5E4R483|A0A5E4R483_ protein OS=Leptidea sinapis 13037.EHJ69303 STAM TY_DN3856_c0_g1_i5.p2 9NEOP OX=189913 GN=LSINAPIS_LOCUS14899 PE=4 SV=1 tr|Q8WSA4|Q8WSA4_SPOFR Signal transducer and activator TRINI- 7091.BGIBMGA00173 tr|Q8WSA4|Q8WSA4_SPOFR of transcription OS=Spodoptera STAT TY_DN1832_c1_g1_i6.p1 9-TA frugiperda OX=7108 GN=STAT PE=2 SV=1 tr|A0A212FKL4|A0A212FKL4_ TRINI- tr|A0A212FKL4|A0A212FKL4_ DANPL Anon-2 OS=Danaus RNAi Pathways TY_DN11178_c0_g1_i1.p 13037.EHJ78324 AGO1 DANPL plexippus plexippus OX=278856 1 GN=KGM_208365 PE=4 SV=1 tr|A0A2H1X4U0|A0A2H1X4U0 _SPOFR Argonaute 1-PCa TRINI- tr|A0A2H1X4U0|A0A2H1X4U0 7091.BGIBMGA00367 OS=Spodoptera frugiperda AGO1 TY_DN7315_c0_g1_i5.p1 _SPOFR 0-TA OX=7108 GN=SFRICE_000078 PE=2 SV=1 tr|A0A2H1X4U0|A0A2H1X4U0 _SPOFR Argonaute 1-PCa TRINI- tr|A0A2H1X4U0|A0A2H1X4U0 7091.BGIBMGA00367 OS=Spodoptera frugiperda AGO1 TY_DN7315_c0_g1_i1.p1 _SPOFR 0-TA OX=7108 GN=SFRICE_000078 PE=2 SV=1 XP_026484156.1 protein argo- TRINI- 7091.BGIBMGA01040 XP_026484156.1 naute-2-like isoform X2 AGO2 TY_DN93_c1_g1_i2.p1 6-TA [Vanessa tameamea] TRINI- XP_026495603.1 piwi-like 34740.HMEL007215- TY_DN14381_c1_g1_i10. XP_026495603.1 protein Ago3 [Vanessa AGO3 PA p1 tameamea] tr|S4NP35|S4NP35_9NEOP TRINI- Arsenite-resistance protein 34740.HMEL013320- tr|S4NP35|S4NP35_9NEOP ARS2 TY_DN2418_c0_g2_i2.p1 (Fragment) OS=Pararge aegeria PA OX=116150 PE=4 SV=1

XP_017149207.1 PREDICTED: TRINI- serrate RNA effector molecule XP_017149207.1 13037.EHJ77624 ARS2 TY_DN2418_c0_g1_i2.p2 homolog isoform X3 [Drosophi- la miranda] TRINI- tr|S4PEK0|S4PEK0_9NEOP 7091.BGIBMGA01064 TY_DN2904_c0_g1_i11.p tr|S4PEK0|S4PEK0_9NEOP Piwi OS=Pararge aegeria AUB 4-TA 1 OX=116150 PE=3 SV=1 TRINI- tr|C9DTM4|C9DTM4_BOMMO 7091.BGIBMGA01246 TY_DN1298_c0_g1_i17.p tr|C9DTM4|C9DTM4_BOMMO CAP isoform A OS=Bombyx CAP 7-TA 2 mori OX=7091 PE=2 SV=1 tr|A0A2H1VGB1|A0A2H1VGB TRINI- 1_SPOFR SFRICE_004635 tr|A0A2H1VGB1|A0A2H1VGB TY_DN1298_c0_g1_i13.p OS=Spodoptera frugiperda 13037.EHJ76670 CAP 1_SPOFR 1 OX=7108 GN=SFRICE_004635 PE=4 SV=1 tr|C9DTM4|C9DTM4_BOMMO TRINI- 7091.BGIBMGA01246 tr|C9DTM4|C9DTM4_BOMMO CAP isoform A OS=Bombyx CAP TY_DN1298_c0_g2_i6.p1 7-TA mori OX=7091 PE=2 SV=1 27

tr|C9DTM4|C9DTM4_BOMMO TRINI- 7091.BGIBMGA01246 tr|C9DTM4|C9DTM4_BOMMO CAP isoform A OS=Bombyx CAP TY_DN1298_c0_g2_i4.p2 7-TA mori OX=7091 PE=2 SV=1 TRINI- tr|C9DTM4|C9DTM4_BOMMO 7091.BGIBMGA01246 TY_DN1298_c0_g1_i26.p tr|C9DTM4|C9DTM4_BOMMO CAP isoform A OS=Bombyx CAP 7-TA 2 mori OX=7091 PE=2 SV=1 tr|A0A212EKQ6|A0A212EKQ6 _DANPL Structural maintenance TRINI- tr|A0A212EKQ6|A0A212EKQ6 of chromosomes protein 13037.EHJ64697 CAP TY_DN2234_c0_g1_i1.p1 _DANPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_205298 PE=3 SV=1 UniRef90_A0A430QE12 TRINI- Uncharacterized protein n=1 34740.HMEL017572- TY_DN10028_c0_g1_i2.p UniRef90_A0A430QE12 Tax=Schistosoma bovis CAP-D2 PA 2 TaxID=6184 RepID=A0A430QE12_SCHBO tr|A0A2A4IXN3|A0A2A4IXN3_ HELVI Nudix hydrolase do- TRINI- tr|A0A2A4IXN3|A0A2A4IXN3_ main-containing protein 34740.HMEL017572- TY_DN10028_c0_g1_i1.p CAP-D2 HELVI OS=Heliothis virescens PA 1 OX=7102 GN=B5V51_11387 PE=4 SV=1 tr|S4NYG7|S4NYG7_9NEOP TRINI- Condensin complex subunit 1 34740.HMEL017572- TY_DN12611_c0_g1_i3.p tr|S4NYG7|S4NYG7_9NEOP CAP-D2 (Fragment) OS=Pararge aegeria PA 2 OX=116150 PE=4 SV=1 TRINI- XP_026499565.1 condensin 34740.HMEL017572- TY_DN12611_c0_g1_i4.p XP_026499565.1 complex subunit 1 isoform X2 CAP-D2 PA 1 [Vanessa tameamea] XP_026501329.1 condensin TRINI- XP_026501329.1 complex subunit 3 [Vanessa 13037.EHJ79105 CAP-G TY_DN1423_c2_g1_i1.p1 tameamea] tr|S4PM57|S4PM57_9NEOP TRINI- Protein CLP1 homolog 34740.HMEL004954- tr|S4PM57|S4PM57_9NEOP CBC TY_DN6154_c0_g2_i6.p1 OS=Pararge aegeria OX=116150 PA PE=3 SV=1 TRINI- XP_026496410.1 endoribonu- TY_DN4256_c0_g1_i18.p XP_026496410.1 clease Dicer [Vanessa 13037.EHJ65725 DCR-2 1 tameamea] XP_026757235.1 enhancer of TRINI- 34740.HMEL012843- XP_026757235.1 mRNA-decapping protein 4-like GE-1 TY_DN6779_c0_g1_i5.p2 PA [Galleria mellonella] XP_026498695.1 enhancer of TRINI- 34740.HMEL012843- XP_026498695.1 mRNA-decapping protein 4-like, GE-1 TY_DN6485_c0_g1_i1.p1 PA partial [Vanessa tameamea] XP_026498695.1 enhancer of TRINI- 34740.HMEL012843- XP_026498695.1 mRNA-decapping protein 4-like, GE-1 TY_DN6485_c0_g2_i4.p1 PA partial [Vanessa tameamea] XP_026498695.1 enhancer of TRINI- 34740.HMEL012843- XP_026498695.1 mRNA-decapping protein 4-like, GE-1 TY_DN6485_c0_g2_i1.p1 PA partial [Vanessa tameamea] XP_026498695.1 enhancer of TRINI- 34740.HMEL012843- XP_026498695.1 mRNA-decapping protein 4-like, GE-1 TY_DN6485_c0_g1_i4.p1 PA partial [Vanessa tameamea] tr|S4P9P6|S4P9P6_9NEOP TRINI- Maternal expression at 31B tr|S4P9P6|S4P9P6_9NEOP 13037.EHJ66501 ME31B TY_DN1576_c0_g1_i7.p1 OS=Pararge aegeria OX=116150 PE=3 SV=1 tr|A0A212FCV3|A0A212FCV3_ DANPL Nuclear transcription TRINI- tr|A0A212FCV3|A0A212FCV3_ factor Y subunit beta 34740.HMEL005908- TY_DN5929_c0_g1_i18.p NF-YB DANPL OS=Danaus plexippus plexippus PA 2 OX=278856 GN=KGM_210110 PE=4 SV=1 tr|A0A2P1DM87|A0A2P1DM87 TRINI- tr|A0A2P1DM87|A0A2P1DM87 _SPOFR Pasha-PA 34740.HMEL014155- TY_DN4101_c0_g1_i12.p PASHA _SPOFR OS=Spodoptera frugiperda PA 1 OX=7108 PE=2 SV=1

tr|A0A2P1DM87|A0A2P1DM87 TRINI- tr|A0A2P1DM87|A0A2P1DM87 _SPOFR Pasha-PA 34740.HMEL014155- PASHA TY_DN4101_c0_g1_i7.p1 _SPOFR OS=Spodoptera frugiperda PA OX=7108 PE=2 SV=1

tr|A0A2P1DMB2|A0A2P1DMB TRINI- tr|A0A2P1DMB2|A0A2P1DMB 2_SPOFR Pasha-PB 34740.HMEL014155- PASHA TY_DN544_c0_g1_i8.p1 2_SPOFR OS=Spodoptera frugiperda PA OX=7108 PE=2 SV=1

tr|A0A2P1DM87|A0A2P1DM87 TRINI- tr|A0A2P1DM87|A0A2P1DM87 _SPOFR Pasha-PA TY_DN23547_c0_g2_i2.p 13037.EHJ71192 PASHA _SPOFR OS=Spodoptera frugiperda 1 OX=7108 PE=2 SV=1 28

TRINI- tr|S4PLX2|S4PLX2_9NEOP TY_DN64761_c0_g1_i1.p tr|S4PLX2|S4PLX2_9NEOP Protein TIS11 OS=Pararge 13037.EHJ75049 TIS11 1 aegeria OX=116150 PE=4 SV=1 XP_026499957.1 translin- TRINI- XP_026499957.1 associated protein X [Vanessa 13037.EHJ78868 TRAX TY_DN8496_c0_g1_i1.p1 tameamea] tr|S4PMD3|S4PMD3_9NEOP TRINI- 34740.HMEL011178- tr|S4PMD3|S4PMD3_9NEOP Translin OS=Pararge aegeria TRSN TY_DN4295_c0_g1_i1.p1 PA OX=116150 PE=4 SV=1 XP_013137504.1 PREDICTED: TRINI- translin-associated factor X- 34740.HMEL010433- TSNAX- TY_DN14022_c0_g1_i1.p XP_013137504.1 interacting protein 1-like isoform PA IP1 1 X1 [Papilio polytes]

XP_026496515.1 translin- TRINI- associated factor X-interacting TSNAX- TY_DN4097_c1_g1_i11.p XP_026496515.1 13037.EHJ64431 protein 1-like isoform X1 IP1 1 [Vanessa tameamea]

XP_028037332.1 activating TRINI- molecule in BECN1-regulated Autophagy TY_DN1295_c4_g1_i22.p XP_028037332.1 13037.EHJ65700 AMBRA1 autophagy protein 1-like, partial 1 [Bombyx mandarina] tr|A0A212FL83|A0A212FL83_ DANPL Autophagy-related TRINI- tr|A0A212FL83|A0A212FL83_ protein 101 isoform 1 TY_DN8057_c0_g1_i12.p 13037.EHJ78617 ATG DANPL OS=Danaus plexippus plexippus 2 OX=278856 GN=KGM_207822 PE=4 SV=1

tr|A0A3S2LP30|A0A3S2LP30_ CHISP Protein kinase domain- TRINI- tr|A0A3S2LP30|A0A3S2LP30_ 34740.HMEL017558- containing protein OS=Chilo ATG1 TY_DN5795_c0_g2_i1.p1 CHISP PA suppressalis OX=168631 GN=evm_004421 PE=4 SV=1

tr|A0A0E3IQG1|A0A0E3IQG1_ SPOLT Autophagy related TRINI- tr|A0A0E3IQG1|A0A0E3IQG1_ protein 1 isoform A 34740.HMEL017558- ATG1 TY_DN5795_c0_g1_i8.p1 SPOLT OS=Spodoptera litura PA OX=69820 GN=Atg1 PE=2 SV=1 tr|A0A0E3IQG1|A0A0E3IQG1_ SPOLT Autophagy related TRINI- tr|A0A0E3IQG1|A0A0E3IQG1_ protein 1 isoform A 34740.HMEL017558- ATG1 TY_DN5795_c0_g1_i1.p1 SPOLT OS=Spodoptera litura PA OX=69820 GN=Atg1 PE=2 SV=1 tr|A0A0E3IQG1|A0A0E3IQG1_ SPOLT Autophagy related TRINI- tr|A0A0E3IQG1|A0A0E3IQG1_ protein 1 isoform A 34740.HMEL017558- ATG1 TY_DN5795_c0_g2_i2.p1 SPOLT OS=Spodoptera litura PA OX=69820 GN=Atg1 PE=2 SV=1

tr|A0A3S2LP30|A0A3S2LP30_ CHISP Protein kinase domain- TRINI- tr|A0A3S2LP30|A0A3S2LP30_ 34740.HMEL017558- containing protein OS=Chilo ATG1 TY_DN5795_c0_g1_i3.p1 CHISP PA suppressalis OX=168631 GN=evm_004421 PE=4 SV=1

tr|A0A3S2LP30|A0A3S2LP30_ CHISP Protein kinase domain- TRINI- tr|A0A3S2LP30|A0A3S2LP30_ 34740.HMEL017558- containing protein OS=Chilo ATG1 TY_DN5795_c0_g1_i6.p1 CHISP PA suppressalis OX=168631 GN=evm_004421 PE=4 SV=1

XP_026489976.1 ubiquitin-like- TRINI- 34740.HMEL014699- XP_026489976.1 conjugating enzyme ATG10 ATG10 TY_DN6464_c0_g1_i1.p1 PA [Vanessa tameamea]

tr|A0A212FM20|A0A212FM20_ TRINI- DANPL Ubiquitin-like protein tr|A0A212FM20|A0A212FM20_ TY_DN15067_c0_g1_i1.p ATG12 OS=Danaus plexippus 13037.EHJ78946 ATG12 DANPL 2 plexippus OX=278856 GN=KGM_201156 PE=3 SV=1

TRINI- XP_026500696.1 autophagy- 34740.HMEL014081- TY_DN10240_c0_g2_i4.p XP_026500696.1 related protein 13 homolog ATG13 PA 1 [Vanessa tameamea] tr|A0A194QEL5|A0A194QEL5_ PAPXU Autophagy-related TRINI- tr|A0A194QEL5|A0A194QEL5_ 34740.HMEL006890- protein 13 OS=Papilio xuthus ATG13 TY_DN2734_c0_g1_i1.p1 PAPXU PA OX=66420 GN=RR46_01874 PE=3 SV=1 29

tr|A0A212F7X6|A0A212F7X6_ DANPL Beclin 1-associated TRINI- tr|A0A212F7X6|A0A212F7X6_ autophagy-related key regulator TY_DN13149_c0_g1_i10. 13037.EHJ73373 ATG14 DANPL like protein OS=Danaus plexip- p1 pus plexippus OX=278856 GN=KGM_201835 PE=4 SV=1 XP_026485297.1 autophagy- TRINI- XP_026485297.1 related protein 16-1 isoform X1 13037.EHJ73902 ATG16 TY_DN7159_c0_g1_i6.p1 [Vanessa tameamea] XP_026485298.1 autophagy- TRINI- XP_026485298.1 related protein 16-1 isoform X2 13037.EHJ73902 ATG16 TY_DN7159_c0_g1_i2.p1 [Vanessa tameamea] XP_026494174.1 autophagy- TRINI- 34740.HMEL002592- XP_026494174.1 related protein 2 homolog A ATG2 TY_DN287_c0_g1_i1.p1 PA [Vanessa tameamea] tr|J9XR17|J9XR17_SPOLT TRINI- Autophagy-related protein 3 34740.HMEL003955- TY_DN1043_c0_g1_i12.p tr|J9XR17|J9XR17_SPOLT ATG3 OS=Spodoptera litura PA 1 OX=69820 PE=2 SV=1 tr|A0A212F652|A0A212F652_D TRINI- ANPL Cysteine protease tr|A0A212F652|A0A212F652_D TY_DN10704_c0_g1_i1.p OS=Danaus plexippus plexippus 13037.EHJ72670 ATG4 ANPL 1 OX=278856 GN=KGM_214126 PE=3 SV=1 TRINI- tr|S4P9P2|S4P9P2_9NEOP TY_DN10704_c0_g1_i2.p tr|S4P9P2|S4P9P2_9NEOP Cysteine protease OS=Pararge 13037.EHJ72670 ATG4 1 aegeria OX=116150 PE=3 SV=1 tr|S4NY94|S4NY94_9NEOP TRINI- Autophagy protein 5 TY_DN8309_c0_g1_i14.p tr|S4NY94|S4NY94_9NEOP 13037.EHJ70753 ATG5 OS=Pararge aegeria OX=116150 2 PE=3 SV=1 TRINI- XP_026484035.1 beclin-1-like XP_026484035.1 13037.EHJ78273 ATG6 TY_DN3118_c0_g1_i1.p1 protein [Vanessa tameamea]

XP_026493535.1 ubiquitin-like TRINI- 34740.HMEL004052- XP_026493535.1 modifier-activating enzyme ATG7 TY_DN1992_c0_g1_i1.p1 PA ATG7 [Vanessa tameamea] tr|A0A1Z2TIV3|A0A1Z2TIV3_ SPOFR Autophagy related TRINI- tr|A0A1Z2TIV3|A0A1Z2TIV3_ 34740.HMEL006641- protein 8 OS=Spodoptera ATG8A TY_DN9625_c0_g1_i1.p1 SPOFR PA frugiperda OX=7108 GN=atg8 PE=2 SV=1 tr|A0A2H1VA96|A0A2H1VA96 _SPOFR Autophagy-related TRINI- tr|A0A2H1VA96|A0A2H1VA96 protein 9 OS=Spodoptera 34740.HMEL008818- TY_DN10151_c0_g1_i1.p ATG9 _SPOFR frugiperda OX=7108 PA 1 GN=SFRICE_002409 PE=3 SV=1 XP_014362846.1 PREDICTED: TRINI- LOW QUALITY PROTEIN: src COR- XP_014362846.1 13037.EHJ64520 TY_DN7014_c0_g2_i1.p1 substrate protein p85 [Papilio TACTIN machaon]

UniRef90_A0A1B6DG41 Ig- like domain-containing protein TRINI- UniRef90_A0A1B6DG41 (Fragment) n=1 Tax=Clastoptera 13037.EHJ77866 DSCAM TY_DN9473_c0_g2_i7.p1 arizonana TaxID=38151 RepID=A0A1B6DG41_9HEMI

UniRef90_A0A482WYE9 Ig- TRINI- like domain-containing protein TY_DN9473_c0_g2_i61.p UniRef90_A0A482WYE9 n=1 Tax=Laodelphax striatellus 7070.TC012539-PA DSCAM 1 TaxID=195883 RepID=A0A482WYE9_LAOST tr|A0A0S7IIK6|A0A0S7IIK6_9T TRINI- ELE DSCAM (Fragment) tr|A0A0S7IIK6|A0A0S7IIK6_9T TY_DN9473_c0_g2_i17.p OS=Poeciliopsis prolifica 7070.TC012539-PA DSCAM ELE 1 OX=188132 GN=DSCAM PE=4 SV=1

tr|A0A0R4IPD4|A0A0R4IPD4_ TRINI- DANRE Down syndrome cell tr|A0A0R4IPD4|A0A0R4IPD4_ TY_DN1715_c1_g1_i45.p adhesion molecule-like 1 (Frag- 7070.TC012539-PA DSCAM DANRE 2 ment) OS=Danio rerio OX=7955 GN=dscaml1 PE=4 SV=1

UniRef90_A0A1Y1MDB4 Uncharacterized protein (Frag- TRINI- ment) n=1 Tax=Photinus pyralis TY_DN24487_c0_g2_i6.p UniRef90_A0A1Y1MDB4 13037.EHJ77866 DSCAM TaxID=7054 1 RepID=A0A1Y1MDB4_PHOP Y tr|A0A0F6NDA7|A0A0F6NDA7 TRINI- _OSTNU Dscam (Fragment) tr|A0A0F6NDA7|A0A0F6NDA7 TY_DN24209_c0_g2_i3.p OS=Ostrinia nubilalis 7070.TC012539-PA DSCAM _OSTNU 1 OX=29057 GN=dscam PE=4 SV=1 30

tr|A0A0F6NCU9|A0A0F6NCU9 TRINI- _OSTNU Dscam (Fragment) tr|A0A0F6NCU9|A0A0F6NCU9 34740.HMEL007149- TY_DN11574_c0_g1_i20. OS=Ostrinia nubilalis DSCAM _OSTNU PA p1 OX=29057 GN=dscam PE=4 SV=1 UniRef90_R9W0H4 Dscam TRINI- (Fragment) n=1 Tax=Maladera 34740.HMEL007149- TY_DN17986_c0_g1_i7.p UniRef90_R9W0H4 DSCAM orientalis TaxID=941252 PA 1 RepID=R9W0H4_9SCAR tr|A0A0F6NDJ4|A0A0F6NDJ4_ TRINI- OSTNU Dscam (Fragment) tr|A0A0F6NDJ4|A0A0F6NDJ4_ 34740.HMEL007149- TY_DN11574_c0_g1_i10. OS=Ostrinia nubilalis DSCAM OSTNU PA p3 OX=29057 GN=dscam PE=4 SV=1 tr|A0A0F6NDJ4|A0A0F6NDJ4_ TRINI- OSTNU Dscam (Fragment) tr|A0A0F6NDJ4|A0A0F6NDJ4_ 34740.HMEL007149- TY_DN11574_c0_g1_i15. OS=Ostrinia nubilalis DSCAM OSTNU PA p2 OX=29057 GN=dscam PE=4 SV=1 tr|A0A0F6NCS4|A0A0F6NCS4 TRINI- _HELAM Dscam (Fragment) tr|A0A0F6NCS4|A0A0F6NCS4 34740.HMEL007149- TY_DN11574_c0_g1_i2.p OS=Helicoverpa armigera DSCAM _HELAM PA 2 OX=29058 GN=dscam PE=4 SV=1 tr|A0A0F6NCP9|A0A0F6NCP9 TRINI- _HELAM Dscam (Fragment) tr|A0A0F6NCP9|A0A0F6NCP9 TY_DN24209_c0_g2_i5.p OS=Helicoverpa armigera 7165.AGAP010884-PA DSCAM _HELAM 1 OX=29058 GN=dscam PE=4 SV=1 tr|A0A0F6NDJ1|A0A0F6NDJ1_ TRINI- OSTNU Dscam (Fragment) tr|A0A0F6NDJ1|A0A0F6NDJ1_ TY_DN24209_c0_g2_i1.p OS=Ostrinia nubilalis 7213.XP_004520249.1 DSCAM OSTNU 5 OX=29057 GN=dscam PE=4 SV=1 tr|A0A0S7ET52|A0A0S7ET52_ TRINI- 9TELE DSCAM (Fragment) tr|A0A0S7ET52|A0A0S7ET52_ 34740.HMEL002841- TY_DN63204_c0_g1_i1.p OS=Poeciliopsis prolifica DSCAM 9TELE PA 1 OX=188132 GN=DSCAM PE=4 SV=1 tr|A0A0F6NDL0|A0A0F6NDL0 TRINI- _SPOLT Dscam (Fragment) tr|A0A0F6NDL0|A0A0F6NDL0 34740.HMEL007149- TY_DN11574_c0_g1_i10. OS=Spodoptera litura DSCAM _SPOLT PA p4 OX=69820 GN=dscam PE=4 SV=1 UniRef90_R9W2R3 Dscam TRINI- (Fragment) n=2 TY_DN17986_c0_g1_i4.p UniRef90_R9W2R3 Tax=Obtectomera 13037.EHJ73141 DSCAM 2 TaxID=104431 RepID=R9W2R3_CHISP tr|A0A0F6NDA9|A0A0F6NDA9 TRINI- _OSTNU Dscam (Fragment) tr|A0A0F6NDA9|A0A0F6NDA9 34740.HMEL007149- TY_DN11574_c0_g1_i10. OS=Ostrinia nubilalis DSCAM _OSTNU PA p2 OX=29057 GN=dscam PE=4 SV=1 tr|Q95W07|Q95W07_DROYA TRINI- DSCAM splice variant 4.7 TY_DN24860_c0_g1_i3.p tr|Q95W07|Q95W07_DROYA 7227.FBpp0110354 DSCAM (Fragment) OS=Drosophila 1 yakuba OX=7245 PE=4 SV=1 tr|A0A0F6NCS9|A0A0F6NCS9 TRINI- _HELAM Dscam (Fragment) tr|A0A0F6NCS9|A0A0F6NCS9 34740.HMEL007149- TY_DN24209_c0_g1_i3.p OS=Helicoverpa armigera DSCAM _HELAM PA 1 OX=29058 GN=dscam PE=4 SV=1 TRINI- AII71600.1 Dscam, partial 34740.HMEL007149- TY_DN24209_c0_g1_i4.p AII71600.1 DSCAM [Helicoverpa armigera] PA 1 tr|A0A0F6NCU0|A0A0F6NCU0 TRINI- _OSTNU Dscam (Fragment) tr|A0A0F6NCU0|A0A0F6NCU0 TY_DN24209_c0_g2_i14. OS=Ostrinia nubilalis 7165.AGAP010884-PA DSCAM _OSTNU p1 OX=29057 GN=dscam PE=4 SV=1 UniRef90_Q95W05 DSCAM TRINI- splice variant 4.12 (Fragment) 132113.XP_003492247. TY_DN30171_c0_g1_i1.p UniRef90_Q95W05 n=1 Tax=Drosophila yakuba DSCAM 1 1 TaxID=7245 RepID=Q95W05_DROYA tr|A0A0F6NDA9|A0A0F6NDA9 TRINI- _OSTNU Dscam (Fragment) tr|A0A0F6NDA9|A0A0F6NDA9 34740.HMEL007149- TY_DN11574_c0_g1_i11. OS=Ostrinia nubilalis DSCAM _OSTNU PA p2 OX=29057 GN=dscam PE=4 SV=1 tr|A0A0F6NDI4|A0A0F6NDI4_ TRINI- HELAM Dscam (Fragment) tr|A0A0F6NDI4|A0A0F6NDI4_ 7091.BGIBMGA01036 DSCAML TY_DN11574_c0_g1_i7.p OS=Helicoverpa armigera HELAM 3-TA 1 1 OX=29058 GN=dscam PE=4 SV=1 31

tr|A0A0F6NDJ1|A0A0F6NDJ1_ TRINI- OSTNU Dscam (Fragment) tr|A0A0F6NDJ1|A0A0F6NDJ1_ 7091.BGIBMGA01036 DSCAML TY_DN24209_c0_g2_i2.p OS=Ostrinia nubilalis OSTNU 3-TA 1 1 OX=29057 GN=dscam PE=4 SV=1 tr|A0A212FF76|A0A212FF76_D TRINI- ANPL Tuberin like protein tr|A0A212FF76|A0A212FF76_D TY_DN6895_c1_g2_i12.p OS=Danaus plexippus plexippus 13037.EHJ76090 GIG ANPL 1 OX=278856 GN=KGM_209357 PE=4 SV=1 tr|A0A212FF76|A0A212FF76_D ANPL Tuberin like protein TRINI- tr|A0A212FF76|A0A212FF76_D OS=Danaus plexippus plexippus 13037.EHJ76090 GIG TY_DN6895_c1_g2_i1.p1 ANPL OX=278856 GN=KGM_209357 PE=4 SV=1 tr|A0A212FF76|A0A212FF76_D ANPL Tuberin like protein TRINI- tr|A0A212FF76|A0A212FF76_D OS=Danaus plexippus plexippus 13037.EHJ76090 GIG TY_DN6895_c1_g2_i8.p1 ANPL OX=278856 GN=KGM_209357 PE=4 SV=1 tr|A0A212FF76|A0A212FF76_D TRINI- ANPL Tuberin like protein tr|A0A212FF76|A0A212FF76_D TY_DN6895_c1_g2_i14.p OS=Danaus plexippus plexippus 13037.EHJ76090 GIG ANPL 1 OX=278856 GN=KGM_209357 PE=4 SV=1 tr|A0A212ESQ2|A0A212ESQ2_ DANPL Phosphoinositide 3- TRINI- tr|A0A212ESQ2|A0A212ESQ2_ kinase regulatory subunit 4 13037.EHJ67557 IRD1 TY_DN1936_c1_g1_i1.p1 DANPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_202575 PE=4 SV=1 XP_026492945.1 inhibitor of TRINI- nuclear factor kappa-B kinase 34740.HMEL007884- XP_026492945.1 IRD5 TY_DN9154_c0_g1_i1.p1 subunit alpha [Vanessa PA tameamea] tr|A0A2A4JI47|A0A2A4JI47_H ELVI PI3K/PI4K domain- TRINI- tr|A0A2A4JI47|A0A2A4JI47_H containing protein (Fragment) 34740.HMEL022673- TY_DN12294_c0_g1_i1.p PI3K/PI4K ELVI OS=Heliothis virescens PA 1 OX=7102 GN=B5V51_1536 PE=3 SV=1 tr|A0A2W1BTL8|A0A2W1BTL 8_HELAM PI3K/PI4K domain- TRINI- tr|A0A2W1BTL8|A0A2W1BTL containing protein 34740.HMEL003893- TY_DN1654_c0_g1_i33.p PI3K/PI4K 8_HELAM OS=Helicoverpa armigera PA 1 OX=29058 GN=HaOG205269 PE=3 SV=1

tr|A0A194RM97|A0A194RM97 _PAPMA Serine/threonine- TRINI- tr|A0A194RM97|A0A194RM97 protein kinase TOR OS=Papilio 13037.EHJ65030 TOR TY_DN6686_c0_g1_i3.p1 _PAPMA machaon OX=76193 GN=RR48_10596 PE=3 SV=1

TRINI- GBP30109.1 hypothetical 34740.HMEL011351- TY_DN4251_c0_g1_i12.p GBP30109.1 protein EVAR_94952_1 [Eume- TOR PA 1 ta japonica] tr|A0A2H1WT43|A0A2H1WT4 3_SPOFR Serine/threonine- TRINI- tr|A0A2H1WT43|A0A2H1WT4 protein kinase TOR 13037.EHJ65032 TOR TY_DN3809_c0_g1_i2.p1 3_SPOFR OS=Spodoptera frugiperda OX=7108 GN=SFRICE_000977 PE=3 SV=1 XP_028923669.1 apoptosis- TRINI- inducing factor 1, mitochondrial Apoptosis XP_028923669.1 13037.EHJ74960 AIF TY_DN2327_c0_g3_i3.p3 isoform X2 [Ornithorhynchus anatinus] tr|A0A212FC61|A0A212FC61_ DANPL Apoptosis-inducing TRINI- tr|A0A212FC61|A0A212FC61_ factor 1 OS=Danaus plexippus 13037.EHJ74960 AIF TY_DN2327_c0_g3_i6.p1 DANPL plexippus OX=278856 GN=KGM_204643 PE=4 SV=1

tr|A0A212F5H5|A0A212F5H5_ DANPL Apoptosis-inducing TRINI- tr|A0A212F5H5|A0A212F5H5_ 34740.HMEL014727- factor OS=Danaus plexippus AIF TY_DN5226_c0_g1_i3.p1 DANPL PA plexippus OX=278856 GN=KGM_211545 PE=4 SV=1

XP_026501394.1 apoptosis- TRINI- 34740.HMEL008334- XP_026501394.1 inducing factor 3 isoform X1 AIF3 TY_DN425_c0_g1_i10.p1 PA [Vanessa tameamea] TRINI- XP_026491582.1 apoptosis 34740.HMEL016681- TY_DN22201_c0_g2_i1.p XP_026491582.1 API5 inhibitor 5 [Vanessa tameamea] PA 1 32

XP_026486456.1 apoptosis- TRINI- resistant E3 ubiquitin protein TY_DN12952_c0_g2_i21. XP_026486456.1 13037.EHJ69238 AREL1 ligase 1 isoform X1 [Vanessa p1 tameamea] XP_026486456.1 apoptosis- TRINI- resistant E3 ubiquitin protein TY_DN12952_c0_g1_i34. XP_026486456.1 13037.EHJ69238 AREL1 ligase 1 isoform X1 [Vanessa p1 tameamea] XP_026486456.1 apoptosis- TRINI- resistant E3 ubiquitin protein TY_DN12952_c0_g1_i23. XP_026486456.1 13037.EHJ69238 AREL1 ligase 1 isoform X1 [Vanessa p1 tameamea]

tr|A0A194RAV4|A0A194RAV4 TRINI- _PAPMA Apoptotic protease- tr|A0A194RAV4|A0A194RAV4 TY_DN7275_c0_g1_i10.p activating factor 1 OS=Papilio 13037.EHJ68576 ARK _PAPMA 1 machaon OX=76193 GN=RR48_13486 PE=4 SV=1

TRINI- XP_026493991.1 apoptosis- TY_DN6822_c0_g1_i16.p XP_026493991.1 stimulating of p53 protein 2 13037.EHJ73745 ASPP 1 isoform X3 [Vanessa tameamea] TRINI- XP_026493991.1 apoptosis- TY_DN6822_c0_g1_i12.p XP_026493991.1 stimulating of p53 protein 2 13037.EHJ73745 ASPP 1 isoform X3 [Vanessa tameamea] XP_026493994.1 apoptosis- TRINI- XP_026493994.1 stimulating of p53 protein 2 13037.EHJ73745 ASPP TY_DN6822_c0_g1_i1.p1 isoform X5 [Vanessa tameamea] tr|S4P8H1|S4P8H1_9NEOP TRINI- Inhibitor of apoptosis 2 (Frag- 34740.HMEL002562- TY_DN28965_c0_g1_i3.p tr|S4P8H1|S4P8H1_9NEOP BIRC2 ment) OS=Pararge aegeria PA 1 OX=116150 PE=4 SV=1 TRINI- XP_026494958.1 caspase-1-like 34740.HMEL004700- TY_DN13277_c0_g1_i1.p XP_026494958.1 CASP1 [Vanessa tameamea] PA 1 TRINI- XP_026497654.1 caspase-3-like 34740.HMEL007958- TY_DN33844_c0_g1_i4.p XP_026497654.1 CASP3 [Vanessa tameamea] PA 1 tr|F6K5S4|F6K5S4_EUPAU TRINI- Caspase-3 OS=Euphydryas 7091.BGIBMGA00613 TY_DN101554_c0_g1_i1. tr|F6K5S4|F6K5S4_EUPAU CASP3 aurinia OX=104503 GN=Casp-3 1-TA p1 PE=2 SV=1

TRINI- XP_026496646.1 protein draper XP_026496646.1 13037.EHJ75645 DRPR TY_DN7628_c0_g1_i2.p1 [Vanessa tameamea]

XP_026489801.1 putative TRINI- 34740.HMEL002564- XP_026489801.1 inhibitor of apoptosis isoform X1 IAP1 TY_DN1619_c0_g1_i7.p1 PA [Vanessa tameamea] tr|A0A212FNV6|A0A212FNV6 TRINI- _DANPL Caspase Nc tr|A0A212FNV6|A0A212FNV6 TY_DN1947_c0_g1_i10.p OS=Danaus plexippus plexippus 13037.EHJ74014 NC _DANPL 1 OX=278856 GN=KGM_205925 PE=3 SV=1 TRINI- XP_026501135.1 apoptosis 34740.HMEL008655- TY_DN22269_c0_g2_i1.p XP_026501135.1 regulatory protein Siva-like SIVA PA 1 isoform X2 [Vanessa tameamea] tr|A0A194PTD0|A0A194PTD0_ TRINI- PAPXU CLIP-associating tr|A0A194PTD0|A0A194PTD0_ Modulation TY_DN4119_c0_g2_i14.p protein OS=Papilio xuthus 13037.EHJ66282 CHB PAPXU 1 OX=66420 GN=RR46_12706 PE=4 SV=1 TRINI- XP_026483862.1 CLIP- TY_DN4119_c0_g1_i12.p XP_026483862.1 associating protein isoform X2 13037.EHJ66282 CHB 1 [Vanessa tameamea] XP_028173984.1 CLIP- TRINI- 34740.HMEL015868- XP_028173984.1 associating protein [Ostrinia CLASP TY_DN1882_c0_g1_i3.p1 PA furnacalis]

tr|A0A0N0PCT2|A0A0N0PCT2 TRINI- _PAPMA CAP-Gly domain- tr|A0A0N0PCT2|A0A0N0PCT2 34740.HMEL008248- TY_DN114788_c0_g1_i1. containing protein OS=Papilio CLIP _PAPMA PA p1 machaon OX=76193 GN=RR48_01400 PE=4 SV=1

tr|A0A1E1W1N3|A0A1E1W1N 3_PECGO CLIP domain- TRINI- tr|A0A1E1W1N3|A0A1E1W1N containing serine protease 13037.EHJ64711 CLIP TY_DN9026_c0_g1_i1.p2 3_PECGO (Fragment) OS=Pectinophora gossypiella OX=13191 GN=g.16409 PE=3 SV=1 tr|S4PK70|S4PK70_9NEOP TRINI- CLIP domain-containing serine 34740.HMEL002402- tr|S4PK70|S4PK70_9NEOP CLIP TY_DN9026_c0_g1_i3.p1 protease OS=Pararge aegeria PA OX=116150 PE=3 SV=1 33

XP_026489628.1 CLIP domain- TRINI- XP_026489628.1 containing serine protease 2 13037.EHJ78263 CLIP TY_DN379_c0_g1_i48.p1 isoform X2 [Vanessa tameamea] tr|I4DLF7|I4DLF7_PAPXU TRINI- Clip-domain serine protease, tr|I4DLF7|I4DLF7_PAPXU 13037.EHJ63474 CLIP TY_DN7027_c2_g1_i1.p1 family C OS=Papilio xuthus OX=66420 PE=2 SV=1 XP_026489627.1 CLIP domain- TRINI- XP_026489627.1 containing serine protease 2 13037.EHJ78263 CLIP2 TY_DN5016_c0_g1_i1.p1 isoform X1 [Vanessa tameamea] TRINI- XP_026488554.1 CLIP domain- 34740.HMEL010779- TY_DN13075_c0_g1_i2.p XP_026488554.1 containing serine protease 2-like CLIP2 PA 1 [Vanessa tameamea] TRINI- XP_026494275.1 CLIP domain- TY_DN1853_c0_g1_i12.p XP_026494275.1 containing serine protease 2-like 13037.EHJ73834 CLIP2 1 isoform X1 [Vanessa tameamea] TRINI- XP_026489628.1 CLIP domain- TY_DN12759_c0_g1_i13. XP_026489628.1 containing serine protease 2 13037.EHJ78263 CLIP2 p1 isoform X2 [Vanessa tameamea] TRINI- XP_026494184.1 CLIP domain- 34740.HMEL016483- TY_DN40491_c0_g1_i3.p XP_026494184.1 containing serine protease 2-like CLIP2 PA 1 [Vanessa tameamea] XP_026489627.1 CLIP domain- TRINI- XP_026489627.1 containing serine protease 2 13037.EHJ78263 CLIP2 TY_DN379_c0_g1_i39.p1 isoform X1 [Vanessa tameamea] XP_026494208.1 CLIP domain- TRINI- XP_026494208.1 containing serine protease 2-like 13037.EHJ76005 CLIP2 TY_DN3103_c0_g1_i1.p1 [Vanessa tameamea] TRINI- XP_026494275.1 CLIP domain- TY_DN1853_c0_g1_i17.p XP_026494275.1 containing serine protease 2-like 13037.EHJ73834 CLIP2 1 isoform X1 [Vanessa tameamea] tr|S4PKY1|S4PKY1_9NEOP TRINI- CLIP domain-containing serine tr|S4PKY1|S4PKY1_9NEOP 13037.EHJ73832 CLIPB5 TY_DN8912_c0_g1_i2.p1 protease OS=Pararge aegeria OX=116150 PE=3 SV=1

tr|A0A212EW16|A0A212EW16 _DANPL C-type lectin domain- TRINI- tr|A0A212EW16|A0A212EW16 containing protein OS=Danaus 13037.EHJ68817 CLEC TY_DN5575_c0_g1_i1.p1 _DANPL plexippus plexippus OX=278856 GN=KGM_210065 PE=4 SV=1

tr|H9JDF6|H9JDF6_BOMMO TRINI- C-type lectin domain-containing 34740.HMEL003322- tr|H9JDF6|H9JDF6_BOMMO CLEC TY_DN3868_c0_g1_i3.p1 protein OS=Bombyx mori PA OX=7091 PE=4 SV=1

tr|A0A212FLY5|A0A212FLY5_ DANPL C-type lectin domain- TRINI- tr|A0A212FLY5|A0A212FLY5_ 34740.HMEL008543- containing protein OS=Danaus CLEC TY_DN44_c0_g3_i1.p1 DANPL PA plexippus plexippus OX=278856 GN=KGM_215784 PE=4 SV=1

tr|A0A212EUK9|A0A212EUK9 _DANPL C-type lectin domain- TRINI- tr|A0A212EUK9|A0A212EUK9 containing protein OS=Danaus 13037.EHJ68272 CLEC TY_DN6938_c0_g1_i2.p1 _DANPL plexippus plexippus OX=278856 GN=KGM_213295 PE=4 SV=1

tr|A0A2A4JW42|A0A2A4JW42 TRINI- _HELVI C-type lectin domain- tr|A0A2A4JW42|A0A2A4JW42 34740.HMEL017185- TY_DN33395_c0_g1_i4.p containing protein OS=Heliothis CLEC _HELVI PA 1 virescens OX=7102 GN=B5V51_10869 PE=4 SV=1

tr|A0A3S2NBT5|A0A3S2NBT5 _CHISP C-type lectin domain- TRINI- tr|A0A3S2NBT5|A0A3S2NBT5 34740.HMEL003322- containing protein OS=Chilo CLEC TY_DN3868_c0_g1_i2.p1 _CHISP PA suppressalis OX=168631 GN=evm_008474 PE=4 SV=1

tr|A0A5E4Q9I9|A0A5E4Q9I9_9 NEOP C-type lectin domain- TRINI- tr|A0A5E4Q9I9|A0A5E4Q9I9_9 containing protein OS=Leptidea 34740.HMEL008543- CLEC TY_DN44_c0_g4_i2.p1 NEOP sinapis OX=189913 PA GN=LSINAPIS_LOCUS6736 PE=4 SV=1 34

TRINI- XP_023952685.1 C-type lectin CLEC37D XP_023952685.1 13037.EHJ68272 TY_DN6938_c0_g1_i1.p1 37Db-like [Bicyclus anynana] b tr|A0A212F0U5|A0A212F0U5_ TRINI- DANPL C-type lectin 4 tr|A0A212F0U5|A0A212F0U5_ TY_DN13455_c0_g1_i1.p OS=Danaus plexippus plexippus 13037.EHJ70690 CLEC4 DANPL 2 OX=278856 GN=KGM_214223 PE=4 SV=1 tr|A0A212EHU1|A0A212EHU1 TRINI- tr|A0A212EHU1|A0A212EHU1 _DANPL Defensin OS=Danaus 13037.EHJ63539 DEF TY_DN9332_c0_g1_i1.p2 _DANPL plexippus plexippus OX=278856 GN=KGM_211227 PE=4 SV=1

tr|A0A212F9B2|A0A212F9B2_ TRINI- tr|A0A212F9B2|A0A212F9B2_ DANPL Galectin OS=Danaus TY_DN14161_c0_g1_i1.p 13037.EHJ73865 GAL DANPL plexippus plexippus OX=278856 1 GN=KGM_212469 PE=4 SV=1

tr|A0A0N0PA48|A0A0N0PA48 TRINI- tr|A0A0N0PA48|A0A0N0PA48 _PAPXU Galectin OS=Papilio 34740.HMEL010163- GAL TY_DN1482_c0_g1_i1.p1 _PAPXU xuthus OX=66420 PA GN=RR46_01207 PE=4 SV=1 tr|S4PFQ8|S4PFQ8_9NEOP TRINI- 34740.HMEL005121- tr|S4PFQ8|S4PFQ8_9NEOP Galectin OS=Pararge aegeria GALE1 TY_DN5718_c1_g1_i7.p1 PA OX=116150 PE=4 SV=1 TRINI- XP_026497720.1 gloverin-like 7091.BGIBMGA01386 TY_DN3267_c2_g1_i15.p XP_026497720.1 GLV [Vanessa tameamea] 5-TA 1

TRINI- XP_026497749.1 gloverin-like XP_026497749.1 13037.EHJ66058 GLV TY_DN3188_c4_g1_i1.p1 [Vanessa tameamea]

TRINI- XP_026497729.1 gloverin-like 7091.BGIBMGA01386 XP_026497729.1 GLV TY_DN1070_c0_g2_i1.p1 [Vanessa tameamea] 3-TA tr|A0A212F5X3|A0A212F5X3_ TRINI- DANPL Immune-related Hdd1 tr|A0A212F5X3|A0A212F5X3_ 34740.HMEL007377- TY_DN25330_c0_g1_i1.p OS=Danaus plexippus plexippus HDD1 DANPL PA 1 OX=278856 GN=KGM_213324 PE=4 SV=1 tr|O96383|O96383_HYPCU TRINI- Immune-related Hdd13 34740.HMEL010004- tr|O96383|O96383_HYPCU HDD13 TY_DN62_c0_g1_i20.p1 OS=Hyphantria cunea PA OX=39466 PE=2 SV=1

TRINI- XP_032513408.1 hemocytin XP_032513408.1 13037.EHJ75969 HML TY_DN762_c0_g1_i12.p1 [Danaus plexippus plexippus]

TRINI- XP_028159201.1 LOW QUAL- TY_DN14145_c0_g1_i2.p XP_028159201.1 ITY PROTEIN: lebocin-4-like 13037.EHJ64534 LEB4 1 [Ostrinia furnacalis] tr|A0A194QN18|A0A194QN18_ TRINI- tr|A0A194QN18|A0A194QN18_ PAPMA Lysozyme OS=Papilio 13037.EHJ67777 LYZ TY_DN1295_c2_g4_i1.p1 PAPMA machaon OX=76193 GN=RR48_11406 PE=3 SV=1

TRINI- XP_026497725.1 lysozyme-like 34740.HMEL002874- XP_026497725.1 LYZ TY_DN1395_c0_g2_i3.p1 isoform X1 [Vanessa tameamea] PA

TRINI- XP_026495706.1 lysozyme-like 34740.HMEL008220- TY_DN29107_c0_g1_i5.p XP_026495706.1 LYZ [Vanessa tameamea] PA 1 UniRef90_A0A329PFV3 TRINI- Lysozyme n=8 Tax=Bacteria 68219.JNXI01000031_g TY_DN131036_c0_g1_i1. UniRef90_A0A329PFV3 LYZ TaxID=2 ene5514 p1 RepID=A0A329PFV3_9LACT TRINI- XP_026495858.1 lysozyme-like TY_DN21074_c0_g2_i3.p XP_026495858.1 13037.EHJ72779 LYZ [Vanessa tameamea] 1

TRINI- XP_026497727.1 lysozyme-like 34740.HMEL002874- XP_026497727.1 LYZ TY_DN1395_c0_g1_i2.p1 isoform X2 [Vanessa tameamea] PA

TRINI- XP_026495706.1 lysozyme-like TY_DN29107_c0_g1_i1.p XP_026495706.1 13037.EHJ63150 LYZ [Vanessa tameamea] 1

TRINI- XP_026495999.1 lysozyme-like 34740.HMEL016024- XP_026495999.1 LYZ TY_DN2992_c0_g2_i4.p1 isoform X1 [Vanessa tameamea] PA 35

TRINI- XP_023939372.1 lysozyme-like XP_023939372.1 13037.EHJ77779 LYZ TY_DN3144_c0_g2_i7.p1 [Bicyclus anynana]

TRINI- XP_026495858.1 lysozyme-like TY_DN21074_c0_g2_i2.p XP_026495858.1 13037.EHJ72779 LYZ [Vanessa tameamea] 1

TRINI- XP_026495999.1 lysozyme-like 34740.HMEL016024- XP_026495999.1 LYZ TY_DN2992_c0_g1_i1.p1 isoform X1 [Vanessa tameamea] PA TRINI- XP_026497736.1 lysozyme 34740.HMEL002880- TY_DN36408_c0_g1_i3.p XP_026497736.1 LYZ [Vanessa tameamea] PA 1 tr|A0A194QRD1|A0A194QRD1 TRINI- tr|A0A194QRD1|A0A194QRD1 _PAPMA Nitric oxide synthase 7091.BGIBMGA00293 TY_DN15603_c0_g1_i1.p NOS _PAPMA OS=Papilio machaon OX=76193 7-TA 1 GN=RR48_12741 PE=3 SV=1

tr|A0A212EHH7|A0A212EHH7 TRINI- _DANPL Zinc finger protein tr|A0A212EHH7|A0A212EHH7 TY_DN20145_c0_g1_i1.p NANOS-P OS=Danaus plexip- 13037.EHJ63417 NOS _DANPL 1 pus plexippus OX=278856 GN=KGM_205136 PE=3 SV=1

XP_026492020.1 phenoloxidase- TRINI- XP_026492020.1 activating enzyme-like [Vanessa 13037.EHJ73644 PAE TY_DN9916_c0_g1_i1.p1 tameamea] XP_026492021.1 phenoloxidase- TRINI- 34740.HMEL004371- XP_026492021.1 activating enzyme-like [Vanessa PAE TY_DN6016_c0_g1_i1.p1 PA tameamea] XP_026492014.1 phenoloxidase- TRINI- XP_026492014.1 activating enzyme-like [Vanessa 13037.EHJ73646 PAE TY_DN246_c0_g2_i2.p1 tameamea] XP_026492019.1 phenoloxidase- TRINI- 34740.HMEL004369- XP_026492019.1 activating enzyme-like isoform PAE TY_DN1887_c0_g1_i8.p1 PA X4 [Vanessa tameamea] XP_026492014.1 phenoloxidase- TRINI- XP_026492014.1 activating enzyme-like [Vanessa 13037.EHJ73646 PAE TY_DN246_c0_g2_i1.p1 tameamea] XP_026501330.1 phenoloxidase TRINI- 34740.HMEL008342- XP_026501330.1 subunit 2-like [Vanessa PO2 TY_DN514_c0_g1_i2.p1 PA tameamea]

TRINI- GBP70065.1 Phenoloxidase 7091.BGIBMGA01311 GBP70065.1 PO2 TY_DN514_c0_g1_i5.p2 subunit 2 [Eumeta japonica] 5-TA

XP_026501296.1 phenoloxidase TRINI- 34740.HMEL008342- XP_026501296.1 subunit 2-like [Vanessa PO2 TY_DN798_c0_g1_i2.p1 PA tameamea] XP_026492017.1 phenoloxidase- TRINI- 34740.HMEL004369- XP_026492017.1 activating enzyme-like isoform PPAF TY_DN1887_c0_g2_i1.p1 PA X2 [Vanessa tameamea] TRINI- XP_026494663.1 phenoloxidase- TY_DN48030_c0_g1_i2.p XP_026494663.1 activating factor 1-like [Vanessa 13037.EHJ65620 PPAF1 1 tameamea] TRINI- XP_026494146.1 phenoloxidase- 34740.HMEL014233- TY_DN15341_c0_g1_i2.p XP_026494146.1 activating factor 1-like [Vanessa PPAF1 PA 1 tameamea] XP_026494152.1 phenoloxidase- TRINI- 34740.HMEL013468- XP_026494152.1 activating factor 2-like [Vanessa PPAF2 TY_DN1682_c0_g1_i4.p1 PA tameamea] XP_026494156.1 phenoloxidase- TRINI- XP_026494156.1 activating factor 2-like isoform 13037.EHJ70457 PPAF2 TY_DN3730_c0_g1_i7.p1 X2 [Vanessa tameamea] TRINI- XP_026488635.1 phenoloxidase- 34740.HMEL005224- TY_DN10597_c0_g1_i1.p XP_026488635.1 activating factor 2-like [Vanessa PPAF2 PA 1 tameamea] XP_026494152.1 phenoloxidase- TRINI- 34740.HMEL013468- XP_026494152.1 activating factor 2-like [Vanessa PPAF2 TY_DN1682_c0_g2_i8.p1 PA tameamea] XP_026494120.1 phenoloxidase- TRINI- 7091.BGIBMGA01440 XP_026494120.1 activating factor 2-like isoform PPAF2 TY_DN1193_c0_g1_i1.p1 4-TA X1 [Vanessa tameamea] XP_026494121.1 phenoloxidase- TRINI- 7091.BGIBMGA01440 XP_026494121.1 activating factor 2-like isoform PPAF2 TY_DN1193_c0_g1_i4.p1 4-TA X2 [Vanessa tameamea] tr|A0S6A0|A0S6A0_SPOFR TRINI- Prophenoloxidase subunit 1 tr|A0S6A0|A0S6A0_SPOFR 13037.EHJ78993 PPO1 TY_DN1338_c0_g1_i2.p1 OS=Spodoptera frugiperda OX=7108 PE=2 SV=1

tr|A0A194R7A1|A0A194R7A1_ TRINI- tr|A0A194R7A1|A0A194R7A1_ PAPMA Serpin B10 OS=Papilio 13037.EHJ65045 SERPIN TY_DN9055_c0_g1_i7.p1 PAPMA machaon OX=76193 GN=RR48_10716 PE=3 SV=1 36

tr|A0A2A4JPH1|A0A2A4JPH1_ TRINI- HELVI SERPIN domain- tr|A0A2A4JPH1|A0A2A4JPH1_ 34740.HMEL006260- TY_DN3454_c0_g1_i12.p containing protein OS=Heliothis SERPIN HELVI PA 1 virescens OX=7102 GN=B5V51_14139 PE=3 SV=1 tr|A0A5E4Q9S6|A0A5E4Q9S6_ 9NEOP SERPIN domain- TRINI- tr|A0A5E4Q9S6|A0A5E4Q9S6_ containing protein OS=Leptidea 34740.HMEL007592- TY_DN37782_c0_g1_i1.p SERPIN 9NEOP sinapis OX=189913 PA 1 GN=LSINAPIS_LOCUS5986 PE=3 SV=1 tr|A0A212EIQ3|A0A212EIQ3_ DANPL Serpin 1 (Fragment) TRINI- tr|A0A212EIQ3|A0A212EIQ3_ OS=Danaus plexippus plexippus 13037.EHJ63957 SERPIN1 TY_DN8_c27_g1_i3.p1 DANPL OX=278856 GN=KGM_202455B PE=3 SV=1 tr|A0A3S5XAC5|A0A3S5XAC5 TRINI- tr|A0A3S5XAC5|A0A3S5XAC5 _ANTPE Serpin-1 (Fragment) SERPI- TY_DN10653_c0_g1_i11. 13037.EHJ75278 _ANTPE OS=Antheraea pernyi OX=7119 NA11 p1 PE=2 SV=1 TRINI- XP_026497302.1 serpin B3 34740.HMEL007479- SER- XP_026497302.1 TY_DN4402_c0_g1_i1.p1 [Vanessa tameamea] PA PINB3 tr|A0A5E4Q6I3|A0A5E4Q6I3_9 NEOP SERPIN domain- TRINI- tr|A0A5E4Q6I3|A0A5E4Q6I3_9 containing protein OS=Leptidea 34740.HMEL007596- SER- TY_DN1294_c1_g1_i10.p NEOP sinapis OX=189913 PA PIND1 1 GN=LSINAPIS_LOCUS5987 PE=3 SV=1 tr|A0A212ET32|A0A212ET32_ TRINI- tr|A0A212ET32|A0A212ET32_ DANPL Serpin-27 OS=Danaus 34740.HMEL006662- SER- TY_DN20565_c0_g1_i2.p DANPL plexippus plexippus OX=278856 PA PIN27A 1 GN=KGM_202595 PE=3 SV=1

tr|A0A212ET32|A0A212ET32_ TRINI- tr|A0A212ET32|A0A212ET32_ DANPL Serpin-27 OS=Danaus 34740.HMEL006662- SER- TY_DN20565_c0_g1_i9.p DANPL plexippus plexippus OX=278856 PA PIN27A 1 GN=KGM_202595 PE=3 SV=1 tr|A0A437AXM1|A0A437AXM 1_CHISP SERPIN domain- TRINI- tr|A0A437AXM1|A0A437AXM containing protein OS=Chilo 13037.EHJ65951 SERPIN19 TY_DN8950_c0_g1_i1.p1 1_CHISP suppressalis OX=168631 GN=evm_012537 PE=3 SV=1 tr|A0A194R7A1|A0A194R7A1_ TRINI- tr|A0A194R7A1|A0A194R7A1_ PAPMA Serpin B10 OS=Papilio SER- 13037.EHJ65045 TY_DN9055_c0_g2_i1.p1 PAPMA machaon OX=76193 PINB10 GN=RR48_10716 PE=3 SV=1

tr|A0A0N1IMR0|A0A0N1IMR0 TRINI- tr|A0A0N1IMR0|A0A0N1IMR0 _PAPXU Serpin B8 OS=Papilio 34740.HMEL007598- SER- TY_DN565_c0_g1_i1.p1 _PAPXU xuthus OX=66420 PA PINB8 GN=RR46_01513 PE=3 SV=1

UniRef90_A0A0N1IKJ1 Hemi- TRINI- centin-1 n=1 Tax=Papilio Immunoglobulins TY_DN6164_c0_g1_i21.p UniRef90_A0A0N1IKJ1 13037.EHJ72797 HMCN1 machaon TaxID=76193 1 RepID=A0A0N1IKJ1_PAPMA XP_026491834.1 hemicentin-1- TRINI- XP_026491834.1 like isoform X1 [Vanessa 13037.EHJ72797 HMCN1 TY_DN1918_c0_g1_i1.p1 tameamea] XP_032522846.1 hemicentin-1- TRINI- XP_032522846.1 like [Danaus plexippus plexip- 13037.EHJ72797 HMCN1 TY_DN1400_c0_g1_i6.p1 pus]

tr|A0A212F458|A0A212F458_D TRINI- ANPL Hemicentin-1 like protein tr|A0A212F458|A0A212F458_D TY_DN19163_c0_g1_i2.p OS=Danaus plexippus plexippus 13037.EHJ71932 HMCN1 ANPL 1 OX=278856 GN=KGM_209682 PE=4 SV=1

tr|A0A194QAX3|A0A194QAX3 TRINI- tr|A0A194QAX3|A0A194QAX3 _PAPXU Hemicentin-1 34740.HMEL007228- TY_DN36035_c0_g1_i1.p HMCN1 _PAPXU OS=Papilio xuthus OX=66420 PA 1 GN=RR46_07161 PE=4 SV=1

TRINI- XP_026501750.1 hemicentin-1- XP_026501750.1 13037.EHJ71735 HMCN1 TY_DN0_c23_g1_i4.p1 like [Vanessa tameamea]

tr|A0A194RCY1|A0A194RCY1 TRINI- tr|A0A194RCY1|A0A194RCY1 _PAPMA Hemicentin-1 TY_DN104210_c0_g1_i1. 13037.EHJ66252 HMCN1 _PAPMA OS=Papilio machaon OX=76193 p1 GN=RR48_09351 PE=4 SV=1 37

tr|A0A212FKG9|A0A212FKG9 TRINI- _DANPL Hemicentin-1 like tr|A0A212FKG9|A0A212FKG9 34740.HMEL002509- TY_DN10996_c0_g1_i1.p protein OS=Danaus plexippus HMCN1 _DANPL PA 1 plexippus OX=278856 GN=KGM_214568 PE=4 SV=1

TRINI- XP_026490984.1 hemicentin-1- XP_026490984.1 13037.EHJ77338 HMCN1 TY_DN8018_c0_g1_i3.p1 like [Vanessa tameamea]

TRINI- XP_026491840.1 hemicentin-1- TY_DN22647_c0_g1_i1.p XP_026491840.1 13037.EHJ71615 HMCN1 like [Vanessa tameamea] 1 TRINI- XP_026491833.1 hemicentin-1- 34740.HMEL016539- TY_DN1400_c0_g1_i11.p XP_026491833.1 HMCN1 like [Vanessa tameamea] PA 1 TRINI- XP_026500237.1 hemicentin-1- 34740.HMEL010770- TY_DN109135_c0_g1_i1. XP_026500237.1 HMCN1 like [Vanessa tameamea] PA p1 TRINI- XP_026484037.1 hemicentin-1- 34740.HMEL004362- TY_DN15050_c0_g1_i8.p XP_026484037.1 HMCN1 like [Vanessa tameamea] PA 1 TRINI- XP_032517539.1 hemicentin-1 TY_DN14495_c0_g1_i6.p XP_032517539.1 isoform X1 [Danaus plexippus 13037.EHJ76524 HMCN1 1 plexippus] TRINI- XP_032517539.1 hemicentin-1 TY_DN14495_c0_g1_i20. XP_032517539.1 isoform X1 [Danaus plexippus 13037.EHJ76524 HMCN1 p1 plexippus] TRINI- XP_026484037.1 hemicentin-1- 34740.HMEL004362- TY_DN15050_c1_g1_i1.p XP_026484037.1 HMCN1 like [Vanessa tameamea] PA 1 TRINI- XP_026491409.1 hemicentin-1- TY_DN52237_c0_g1_i3.p XP_026491409.1 13037.EHJ78231 HMCN1 like [Vanessa tameamea] 1 TRINI- XP_026498237.1 LOW QUAL- 7091.BGIBMGA00934 TY_DN5451_c0_g1_i11.p XP_026498237.1 ITY PROTEIN: hemicentin-1- HMCN1 3-TA 1 like [Vanessa tameamea] UniRef90_A0A443QZM3 Hemicentin-2-like protein TRINI- (Fragment) n=2 TY_DN147375_c0_g1_i1. UniRef90_A0A443QZM3 13037.EHJ74080 HMCN2 Tax=Dinothrombium tinctorium p1 TaxID=1965070 RepID=A0A443QZM3_9ACAR TRINI- XP_026493434.1 hemicentin-2- TY_DN15528_c1_g1_i18. XP_026493434.1 like isoform X2 [Vanessa 13037.EHJ71990 HMCN2 p1 tameamea] TRINI- XP_026495222.1 hemicentin-2- TY_DN26444_c0_g2_i1.p XP_026495222.1 like isoform X2 [Vanessa 13037.EHJ65737 HMCN2 1 tameamea] TRINI- XP_026493388.1 hemicentin-2- TY_DN11948_c0_g1_i2.p XP_026493388.1 13037.EHJ71990 HMCN2 like [Vanessa tameamea] 1 TRINI- XP_026495406.1 hemicentin-2- TY_DN32230_c0_g1_i1.p XP_026495406.1 13037.EHJ71470 HMCN2 like [Vanessa tameamea] 1

TRINI- XP_026501743.1 hemicentin-1 XP_026501743.1 13037.EHJ76866 NRM TY_DN4766_c0_g1_i6.p1 isoform X4 [Vanessa tameamea]

XP_026491088.1 vascular TRINI- Miscellaneous XP_026491088.1 endothelial growth factor recep- 13037.EHJ78656 PVR TY_DN192_c3_g1_i6.p1 tor 1 [Vanessa tameamea] XP_026491088.1 vascular TRINI- XP_026491088.1 endothelial growth factor recep- 13037.EHJ78656 PVR TY_DN192_c3_g2_i2.p1 tor 1 [Vanessa tameamea]

Table 5. Top 5 up-regulated and top 5 down-regulated transcripts with log2 fold changes, false detection rate (FDR; padj) value, final EnTAP annotation, and description of annotation for each of the four comparison groups. Regulation Transcript LFC (log2) FDR (padj) Final Annotation Description VcB vs. VcS Transcription TRINI- tr|A0A212FHE0| initiation factor Up-regulated TY_DN1820_c0 10.1054 9.1954E-06 A0A212FHE0_ IIF subunit alpha _g1_i1.p1 DANPL (Fragment) TRINI- tr|A0A212ERL5| TY_DN7287_c0 7.8810 7.7472E-05 A0A212ERL5_ Spalt protein _g1_i7.p1 DANPL TRINI- 40S ribosomal TY_DN5247_c0 7.8249 1.0596E-25 GBP72221.1 protein S7 _g2_i7.p1 Retrotrans_gag TRINI- tr|A0A437B3Z9| domain- TY_DN5704_c0 7.3877 4.9572E-02 A0A437B3Z9_C containing pro- _g1_i1.p2 HISP tein homeodomain- TRINI- interacting pro- TY_DN2939_c1 5.4472 6.2642E-03 XP_026490345 tein kinase 2-like _g1_i1.p1 isoform X2 (HIPK) TRINI- XP_023936123. Down-regulated TY_DN3326_c0 -8.9182 3.3017E-06 Liprin-alpha-1 1 _g1_i10.p1 TRINI- XP_026494533. translocator pro- TY_DN2269_c0 -7.7652 6.6004E-05 1 tein isoform X2 _g2_i1.p1 TRINI- tr|S4NT30|S4NT Elongation factor TY_DN18476_c -5.2876 7.3468E-16 30_9NEOP 1 delta 0_g2_i1.p1 TRINI- XP_023936123. TY_DN3326_c0 -4.6745 7.2885E-03 Liprin-alpha-1 1 _g1_i14.p1 10- TRINI- tr|A0A5E4Q650| formyltetrahy- TY_DN1357_c0 -4.1996 8.7771E-03 A0A5E4Q650_9 drofolate dehy- _g1_i4.p1 NEOP drogenase VcV vs. VcN TRINI- XP_026501185. RNA-binding Up-regulated TY_DN2472_c1 20.7738 6.7258E-05 1 protein 45-like _g2_i2.p1 TRINI- uncharacterized XP_032524538. TY_DN227_c3_ 10.0770 2.6190E-11 protein 1 g2_i6.p1 LOC116775692 TRINI- tr|A0A3S2N9E0| Uncharacterized TY_DN910_c0_ 9.2415 2.3985E-09 A0A3S2N9E0_ protein g2_i24.p1 CHISP TRINI- tr|Q9NDV5|Q9N TY_DN4925_c0 8.9301 1.5937E-08 Alpha-amylase DV5_BOMMO _g1_i20.p2 TRINI- tr|S4PBQ2|S4PB TY_DN3012_c2 8.6513 3.9147E-02 Rac1 Q2_9NEOP _g1_i4.p1 TRINI- XP_026492845. larval cuticle Down-regulated -9.2477 2.8982E-04 TY_DN4264_c0 1 protein LCP-30- 40

_g1_i2.p1 like

TRINI- tyrosine-protein XP_026486949. TY_DN2033_c0 -7.7937 1.2326E-05 phosphatase Lar 1 _g1_i6.p1 isoform X3 TRINI- tr|A0A212ERL5| TY_DN7287_c0 -7.7206 1.2068E-04 A0A212ERL5_ Spalt protein _g1_i7.p1 DANPL TRINI- uncharacterized XP_026497692. TY_DN604_c0_ -6.5783 2.0884E-02 protein 1 g1_i23.p1 LOC113401839 TRINI- tr|A0A2A4K7W Uncharacterized TY_DN1421_c0 -5.7916 3.4200E-24 9|A0A2A4K7W protein _g1_i1.p1 9_HELVI VcV vs. VcB TRINI- tr|A0A212FB13| Ufm1- Up-regulated TY_DN3019_c0 9.3572 5.2607E-04 A0A212FB13_D conjugating en- _g1_i1.p1 ANPL zyme 1 TRINI- XP_023936123. TY_DN3326_c0 8.9176 1.4776E-06 Liprin-alpha-1 1 _g1_i10.p1 TRINI- tr|A0A194Q9D0| Drebrin-like TY_DN3451_c0 8.1574 7.4780E-07 A0A194Q9D0_P protein _g1_i11.p1 APXU TPR_REGION TRINI- tr|A0A212FAI4| domain- TY_DN17841_c 7.4725 4.9402E-04 A0A212FAI4_D containing pro- 0_g1_i4.p1 ANPL tein G-protein cou- TRINI- XP_026499602. pled receptor TY_DN10216_c 7.0760 3.5088E-02 1 Mth2-like iso- 0_g2_i2.p1 form X1 Aconitase_C TRINI- tr|A0A315V6M7 domain- Down-regulated TY_DN12384_c -8.8472 3.6314E-03 |A0A315V6M7_ containing pro- 0_g1_i2.p1 GAMAF tein (Fragment) TRINI- larval/pupal rigid XP_026488169. TY_DN5121_c0 -7.9195 3.7396E-06 cuticle protein 1 _g2_i3.p1 66-like TRINI- XP_026497720. TY_DN3267_c2 -7.8658 6.0227E-03 Gloverin-like 1 _g1_i15.p1 TRINI- tr|A0A212ERL5| TY_DN7287_c0 -7.8163 9.3070E-05 A0A212ERL5_ Spalt protein _g1_i7.p1 DANPL inducible metal- TRINI- XP_026485721. loproteinase TY_DN1710_c0 -7.6191 6.7112E-03 1 inhibitor protein- _g1_i17.p2 like VcN vs. VcS leucine-rich TRINI- repeat trans- Up-regulated TY_DN642_c0_ 9.3639 2.5290E-06 XP_026497835 membrane neu- g1_i2.p2 ronal protein 3 TRINI- tr|A0A212EJA6| Short spindle TY_DN3043_c0 9.0873 5.3860E-09 A0A212EJA6_D protein 4 isoform _g1_i40.p1 ANPL 3 TRINI- tr|A0A212EJA6| Short spindle 8.7276 7.0753E-03 TY_DN3043_c0 A0A212EJA6_D protein 4 isoform 41

_g1_i37.p1 ANPL 3

TRINI- tr|A0A212ERL5| TY_DN7287_c0 7.7966 2.3528E-04 A0A212ERL5_ Spalt protein _g1_i7.p1 DANPL TRINI- tr|A0A212EJA6| Short spindle TY_DN3043_c0 7.3312 3.9594E-05 A0A212EJA6_D protein 4 isoform _g1_i2.p1 ANPL 3 acyl-CoA- TRINI- XP_026492429. binding domain- Down-regulated TY_DN2600_c0 -11.6398 1.2409E-17 1 containing pro- _g1_i3.p1 tein 6-like TRINI- UniRef90_B4Q TY_DN200_c1_ -10.4630 5.1106E-07 GD25582 HC7 g1_i1.p2 TRINI- tr|A0A5E4PQ04| LRRCT domain- TY_DN7508_c0 -10.4003 1.2243E-06 A0A5E4PQ04_9 containing pro- _g1_i14.p1 NEOP tein TRINI- tr|S4PBQ2|S4PB TY_DN3012_c2 -8.7796 4.0349E-12 Rac1 Q2_9NEOP _g1_i4.p1 TRINI- uncharacterized XP_026499898. TY_DN27979_c -8.4964 6.8205E-03 protein 1 0_g2_i4.p1 LOC113403532 *Red stars signify transcripts identified as having an immune related function

Table 6. Comparison of all the lepidopteran viruses found in the individual replicates of each treatment (JcDNV in bold). Viruses VcB VcS VcV VcN Name Lineage 1 2 3 1 2 3 1 2 3 1 2 3 Spodoptera frugiperda Ascoviridae; Ascovirus X X X X X X X X X X X X ascovirus 1a Trichoplusia ni ascovirus Ascoviridae; Ascovirus 2a Heliothis virescens Ascoviridae; Ascovirus; Heliothis virescens ascovirus X X X X X X X X X X X ascovirus 3e 3a Heliothis virescens Ascoviridae; Ascovirus; Heliothis virescens ascovirus X X X X X X X X X X X X ascovirus 3f 3a Heliothis virescens Ascoviridae; Ascovirus; Heliothis virescens ascovirus X X X X X X X X X X ascovirus 3g 3a Trichoplusia ni ascovirus Ascoviridae; Ascovirus; unclassified Ascovirus X X X X X X X X X X X X 2c Adoxophyes honmai Baculoviridae; Alphabaculovirus X X X X X X X X X X X nucleopolyhedrovirus Agrotis ipsilon multiple Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Agrotis segetum nucleo- Baculoviridae; Alphabaculovirus X X X X X X X X X polyhedrovirus A Agrotis segetum nucleo- Baculoviridae; Alphabaculovirus X X X X X X X X X X X X polyhedrovirus B Antheraea pernyi nucle- Baculoviridae; Alphabaculovirus X X X X X X X X X X opolyhedrovirus Anticarsia gemmatalis multiple nucleopolyhe- Baculoviridae; Alphabaculovirus X X X X X X drovirus Bombyx mori nucleo- Baculoviridae; Alphabaculovirus X X X X X X X X X X X X polyhedrovirus Buzura suppressaria Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Catopsilia pomona nu- Baculoviridae; Alphabaculovirus X X X X X X X X X X X X cleopolyhedrovirus Choristoneura fumiferana DEF multiple Baculoviridae; Alphabaculovirus X X X X X X X nucleopolyhedrovirus Choristoneura fumiferana multiple nucle- Baculoviridae; Alphabaculovirus X X X X X X X X X X X opolyhedrovirus Choristoneura murinana Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Choristoneura rosaceana Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Chrysodeixis chalcites Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Clanis bilineata nucleo- Baculoviridae; Alphabaculovirus X X X X X X X X X polyhedrovirus Ectropis obliqua nucleo- Baculoviridae; Alphabaculovirus X X X X X X X X X X X X polyhedrovirus Epiphyas postvittana Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Euproctis pseudocon- spersa nucleopolyhe- Baculoviridae; Alphabaculovirus X X X X X X X X X X X X drovirus Helicoverpa armigera Baculoviridae; Alphabaculovirus X X X X X X X X nucleopolyhedrovirus Helicoverpa zea single Baculoviridae; Alphabaculovirus nucleopolyhedrovirus Hyphantria cunea nucle- Baculoviridae; Alphabaculovirus X X X X X X X X X opolyhedrovirus 43

Lambdina fiscellaria Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Leucania separata nucle- Baculoviridae; Alphabaculovirus X X X X X X X X X X X X opolyhedrovirus Lymantria dispar multi- Baculoviridae; Alphabaculovirus X X X X X X X X X X X X ple nucleopolyhedrovirus Lymantria xylina nucle- Baculoviridae; Alphabaculovirus X X X X X X X opolyhedrovirus Mamestra brassicae multiple nucleopolyhe- Baculoviridae; Alphabaculovirus X X X X X X X X X X drovirus Mamestra configurata Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus A Mamestra configurata Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus B Maruca vitrata nucleo- Baculoviridae; Alphabaculovirus X X X X X X X X X X X X polyhedrovirus Mythimna unipuncta Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Operophtera brumata Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Orgyia leucostigma Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Orgyia pseudotsugata multiple nucleopolyhe- Baculoviridae; Alphabaculovirus X X X X X X X X drovirus Oxyplax ochracea nucle- Baculoviridae; Alphabaculovirus X X X X X X X X X X X X opolyhedrovirus Spodoptera exigua multiple nucleopolyhe- Baculoviridae; Alphabaculovirus X X X X X X X X X X X X drovirus Spodoptera frugiperda multiple nucleopolyhe- Baculoviridae; Alphabaculovirus X X X X X X X X X X X drovirus Spodoptera littoralis Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Spodoptera litura nucle- Baculoviridae; Alphabaculovirus X X X X X X X X X X X opolyhedrovirus Sucra jujuba nucleopoly- Baculoviridae; Alphabaculovirus X X X X X X X X X X X hedrovirus Thysanoplusia orichalcea Baculoviridae; Alphabaculovirus X X X X X X X X X X X nucleopolyhedrovirus Trichoplusia ni single Baculoviridae; Alphabaculovirus X X X X X X X X X X X X nucleopolyhedrovirus Wiseana signata nucleo- Baculoviridae; Alphabaculovirus polyhedrovirus Philosamia cynthia ricini Baculoviridae; Alphabaculovirus; Antheraea pernyi nucleopolyhedrovirus nucleopolyhedrovirus virus Autographa californica Baculoviridae; Alphabaculovirus; Autographa califor- X X X X X X X X X X X X nucleopolyhedrovirus nica multiple nucleopolyhedrovirus Plutella xylostella multi- Baculoviridae; Alphabaculovirus; Autographa califor- ple nucleopolyhedrovirus nica multiple nucleopolyhedrovirus Baculoviridae; Alphabaculovirus; Autographa califor- Rachiplusia ou MNPV nica multiple nucleopolyhedrovirus Bombyx mandarina Baculoviridae; Alphabaculovirus; Bombyx mori nucle- nucleopolyhedrovirus opolyhedrovirus Bombyx mandarina Baculoviridae; Alphabaculovirus; Bombyx mori nucle- nucleopolyhedrovirus S2 opolyhedrovirus Choristoneura occiden- Baculoviridae; Alphabaculovirus; Choristoneura fu- talis alphabaculovirus miferana multiple nucleopolyhedrovirus Pseudoplusia includens Baculoviridae; Alphabaculovirus; Chrysodeixis in- X X X X X X X X X X X X SNPV IE cludens nucleopolyhedrovirus Helicoverpa armigera Baculoviridae; Alphabaculovirus; Helicoverpa armigera NPV NNg1 nucleopolyhedrovirus Helicoverpa armigera Baculoviridae; Alphabaculovirus; Helicoverpa armigera X X X X X X X X nucleopolyhedrovirus G4 nucleopolyhedrovirus 44

Hemileuca sp. nucleo- Baculoviridae; Alphabaculovirus; Hemileuca species X X X X X X X X X X X polyhedrovirus nucleopolyhedrovirus Lonomia obliqua multi- Baculoviridae; Alphabaculovirus; Lonomia obliqua X X X X X X X X X X X X ple nucleopolyhedrovirus nucleopolyhedrovirus Helicoverpa armigera Baculoviridae; Alphabaculovirus; Mamestra configura- multiple nucleopolyhe- ta nucleopolyhedrovirus B drovirus Perigonia lusca single Baculoviridae; Alphabaculovirus; Perigonia lusca nu- X X X X X X X X X X X X nucleopolyhedrovirus cleopolyhedrovirus Adoxophyes orana nu- Baculoviridae; Alphabaculovirus; unclassified Al- X X X X X X X X X X X X cleopolyhedrovirus phabaculovirus Anticarsia gemmatalis Baculoviridae; Alphabaculovirus; unclassified Al- X X X X X nucleopolyhedrovirus phabaculovirus Apocheima cinerarium Baculoviridae; Alphabaculovirus; unclassified Al- X X X X X X X X X X X X nucleopolyhedrovirus phabaculovirus Condylorrhiza vestigialis Baculoviridae; Alphabaculovirus; unclassified Al- X X X X X X X X X X X X MNPV phabaculovirus Malacosoma neustria Baculoviridae; Alphabaculovirus; unclassified Al- X X X X X X X X X X X X nucleopolyhedrovirus phabaculovirus Peridroma al- Baculoviridae; Alphabaculovirus; unclassified Al- X X X X X X X X X X X X phabaculovirus phabaculovirus Spodoptera litura nucle- Baculoviridae; Alphabaculovirus; unclassified Al- X X X X X X X X X X X X opolyhedrovirus II phabaculovirus Adoxophyes orana gran- Baculoviridae; Betabaculovirus X X X X X X X X X X X ulovirus Agrotis segetum granu- Baculoviridae; Betabaculovirus X X X X X X X X X X X X lovirus Artogeia rapae granulo- Baculoviridae; Betabaculovirus X X X X X X X X X X X X virus Choristoneura fu- Baculoviridae; Betabaculovirus X X X X X X X X X X X X miferana granulovirus Clostera anachoreta Baculoviridae; Betabaculovirus X X X X X X X X X X X X granulovirus Clostera anastomosis Baculoviridae; Betabaculovirus X X X X X X X X X X X granulovirus B Cnaphalocrocis medi- Baculoviridae; Betabaculovirus X X X X X X X X X X X X nalis granulovirus Cryptophlebia leucotreta Baculoviridae; Betabaculovirus X X X X X X X X X X X X granulovirus Cydia pomonella granu- Baculoviridae; Betabaculovirus X X X X X X X X X X X X lovirus Diatraea saccharalis Baculoviridae; Betabaculovirus X X X X X X X X granulovirus Epinotia aporema granu- Baculoviridae; Betabaculovirus X X X X X X X X X X X lovirus Erinnyis ello granulovi- Baculoviridae; Betabaculovirus X X X X X X X rus Harrisina brillians granu- Baculoviridae; Betabaculovirus lovirus Helicoverpa armigera Baculoviridae; Betabaculovirus X X X X X X X X X X X X granulovirus Lacanobia oleracea Baculoviridae; Betabaculovirus granulovirus Mocis latipes granulovi- Baculoviridae; Betabaculovirus X X X X X X X X X X X X rus Mythimna unipuncta Baculoviridae; Betabaculovirus X X X granulovirus B Phthorimaea operculella Baculoviridae; Betabaculovirus X X X X X X X X X granulovirus Plodia interpunctella Baculoviridae; Betabaculovirus X X X X X X X X X X X granulovirus Plutella xylostella granu- Baculoviridae; Betabaculovirus X X X X X X X X X X X X lovirus Spodoptera frugiperda Baculoviridae; Betabaculovirus X X X X X X X X X X X X granulovirus Spodoptera litura granu- Baculoviridae; Betabaculovirus X X X X X X X X X X X X lovirus 45

Xestia c-nigrum granu- Baculoviridae; Betabaculovirus X X X X X X X X X X X lovirus Clostera anastomosis Baculoviridae; Betabaculovirus; Clostera anastomosis X X X X X X X X X granulovirus Henan granulovirus A Pseudalatia unipuncta Baculoviridae; Betabaculovirus; Mythimna unipuncta X X X X X X X X X X X granulovirus granulovirus A Trichoplusia ni granulo- Baculoviridae; Betabaculovirus; Trichoplusia ni granu- X X X X X X X X X X X X virus LBIV-12 lovirus Urbanus proteus nucleo- Baculoviridae; unclassified Baculoviridae X X X X X X X X X X X X polyhedrovirus Bombyx mori densovirus Bidnaviridae; Bidensovirus; Bombyx mori bidensovirus X X 3 Bombyx mori densovirus Bidnaviridae; Bidensovirus; Bombyx mori bidensovirus X X Zhenjiang Iridoviridae; Betairidovirinae; Chloriridovirus; Inverte- Wiseana iridescent virus X X X X X X X X X X X X brate iridescent virus 9 Invertebrate iridescent Iridoviridae; Betairidovirinae; Chloriridovirus; unclas- X X X X X X X X X X X X virus 30 sified Chloriridovirus Iridoviridae; Betairidovirinae; Chloriridovirus; unclas- Invertebrate iridovirus 22 X X X X X X X X X X X X sified Chloriridovirus Invertebrate iridescent Iridoviridae; Betairidovirinae; Iridovirus X X X X X X X X X X X X virus 6 Heliothis zea nudivirus Nudiviridae; Betanudivirus Helicoverpa zea nudi- Nudiviridae; Betanudivirus; Heliothis zea nudivirus X X X X X X X X X X X X virus 2 Trichoplusia ni TED Ortervirales; Metaviridae; Errantivirus X virus Galleria mellonella den- Parvoviridae; Densovirinae; Ambidensovirus; Lepidop- X X X X X X X X X X sovirus teran ambidensovirus 1 Junonia coenia denso- Parvoviridae; Densovirinae; Ambidensovirus; Lepidop- X X X X X X X X X X virus teran ambidensovirus 1 Mythimna loreyi denso- Parvoviridae; Densovirinae; Ambidensovirus; Lepidop- X X X X X X X X X X X virus teran ambidensovirus 1; Junonia coenia densovirus Pseudoplusia includens Parvoviridae; Densovirinae; Ambidensovirus; Lepidop- densovirus teran ambidensovirus 1; Junonia coenia densovirus Diatraea saccharalis Parvoviridae; Densovirinae; Ambidensovirus; unclassi- X densovirus fied Densovirus Bombyx mori densovirus Parvoviridae; Densovirinae; Iteradensovirus; Lepidop- 1 teran iteradensovirus 1 Bombyx mori densovirus Parvoviridae; Densovirinae; Iteradensovirus; Lepidop- X 5 teran iteradensovirus 1; Bombyx mori densovirus Casphalia extranea den- Parvoviridae; Densovirinae; Iteradensovirus; Lepidop- sovirus teran iteradensovirus 2 Parvoviridae; Densovirinae; Iteradensovirus; Lepidop- Sibine fusca densovirus teran iteradensovirus 2 Dendrolimus punctatus Parvoviridae; Densovirinae; Iteradensovirus; Lepidop- densovirus teran iteradensovirus 3 Papilio polyxenes denso- Parvoviridae; Densovirinae; Iteradensovirus; Lepidop- virus teran iteradensovirus 4 Helicoverpa armigera Parvoviridae; Densovirinae; Iteradensovirus; Lepidop- densovirus teran iteradensovirus 5 Danaus plexippus plex- Parvoviridae; Densovirinae; Iteradensovirus; unclassi- ippus iteravirus fied Iteravirus Campoletis sonorensis Polydnaviridae; Ichnovirus X X X X X X X X X X X X ichnovirus Glypta fumiferanae Polydnaviridae; Ichnovirus X X X X X X X X X X X X ichnovirus Hyposoter fugitivus Polydnaviridae; Ichnovirus X X X X X X X X X X X X ichnovirus Amsacta moorei ento- Poxviridae; Entomopoxvirinae; Betaentomopoxvirus X X X X X X X X X X X X mopoxvirus Choristoneura biennis Poxviridae; Entomopoxvirinae; Betaentomopoxvirus X X X X X X X X X X X X entomopoxvirus Choristoneura fumiferana entomopox- Poxviridae; Entomopoxvirinae; Betaentomopoxvirus virus 46

Adoxophyes honmai Poxviridae; Entomopoxvirinae; Betaentomopoxvirus; X X X X X X X X X X X X entomopoxvirus 'L' Adoxophyes honmai entomopoxvirus Choristoneura rosaceana Poxviridae; Entomopoxvirinae; Betaentomopoxvirus; X X X X X X X X X X X X entomopoxvirus 'L' Choristoneura rosaceana entomopoxvirus Mythimna separata en- Poxviridae; Entomopoxvirinae; Betaentomopoxvirus; X X X X X X X X X X X X tomopoxvirus 'L' Mythimna separata entomopoxvirus Nudaurelia capensis beta Riboviria; Alphatetraviridae; Betatetravirus virus Dendrolimus punctatus Riboviria; Alphatetraviridae; Omegatetravirus X virus Helicoverpa armigera Riboviria; Alphatetraviridae; Omegatetravirus stunt virus Nudaurelia capensis Riboviria; Alphatetraviridae; Omegatetravirus omega virus Providence virus Riboviria; Carmotetraviridae; Alphacarmotetravirus Riboviria; Negarnaviricota; Haploviricotina; Monjiviri- Orinoco virus cetes; Mononegavirales; Nyamiviridae; Orinovirus; Orinoco orinovirus Riboviria; Negarnaviricota; Haploviricotina; Monjiviri- Gata virus cetes; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae Riboviria; Negarnaviricota; Haploviricotina; Monjiviri- Orgi virus cetes; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae Riboviria; Negarnaviricota; Haploviricotina; Monjiviri- Spodoptera frugiperda cetes; Mononegavirales; Rhabdoviridae; unclassified X rhabdovirus Rhabdoviridae Riboviria; Negarnaviricota; Polyploviricotina; El- Shuangao Insect Virus 2 lioviricetes; Bunyavirales; Phasmaviridae; Inshuvirus; Insect inshuvirus Riboviria; Negarnaviricota; Polyploviricotina; El- Seattle Prectang virus lioviricetes; Bunyavirales; Phasmaviridae; Or- thophasmavirus; Seattle orthophasmavirus Riboviria; Negarnaviricota; Polyploviricotina; El- Hubei lepidoptera virus 1 lioviricetes; Bunyavirales; Phenuiviridae; Hudovirus; X X Lepidopteran hudovirus Riboviria; Negarnaviricota; Polyploviricotina; El- Mothra virus lioviricetes; Bunyavirales; Phenuiviridae; Mobuvirus; Mothra mobuvirus Riboviria; Negarnaviricota; Polyploviricotina; El- Pidgey virus lioviricetes; Bunyavirales; Phenuiviridae; Pidchovirus; X Pidgey pidchovirus Boolarra virus Riboviria; Nodaviridae; Alphanodavirus Flock House virus Riboviria; Nodaviridae; Alphanodavirus Nodamura virus Riboviria; Nodaviridae; Alphanodavirus X X X Pariacoto virus Riboviria; Nodaviridae; Alphanodavirus Euprosterna elaeasa virus Riboviria; Permutotetraviridae; Alphapermutotetravirus X X X Thosea asigna virus Riboviria; Permutotetraviridae; Alphapermutotetravirus Antheraea pernyi ifla- Riboviria; Picornavirales; Iflaviridae; Iflavirus X virus Infectious flacherie virus Riboviria; Picornavirales; Iflaviridae; Iflavirus Lymantria dispar ifla- Riboviria; Picornavirales; Iflaviridae; Iflavirus virus 1 Perina nuda virus Riboviria; Picornavirales; Iflaviridae; Iflavirus Spodoptera exigua ifla- Riboviria; Picornavirales; Iflaviridae; Iflavirus virus 1 Spodoptera exigua ifla- Riboviria; Picornavirales; Iflaviridae; Iflavirus virus 2 Ectropis obliqua picorna- Riboviria; Picornavirales; Iflaviridae; Iflavirus; Ectropis like virus obliqua virus Riboviria; Picornavirales; Iflaviridae; Iflavirus; unclas- Bombyx mori iflavirus sified Iflavirus 47

Diamondback moth Riboviria; Picornavirales; Iflaviridae; Iflavirus; unclas- iflavirus sified Iflavirus Riboviria; Picornavirales; Iflaviridae; Iflavirus; unclas- Heliconius erato iflavirus sified Iflavirus Helicoverpa armigera Riboviria; Picornavirales; Iflaviridae; Iflavirus; unclas- iflavirus sified Iflavirus Opsiphanes invirae ifla- Riboviria; Picornavirales; Iflaviridae; unclassified virus 1 Iflaviridae Thaumetopoea Riboviria; Picornavirales; Iflaviridae; unclassified X pityocampa iflavirus 1 Iflaviridae Cypovirus 1 Riboviria; Reoviridae; Spinareovirinae; Cypovirus Cypovirus 14 Riboviria; Reoviridae; Spinareovirinae; Cypovirus Cypovirus 5 Riboviria; Reoviridae; Spinareovirinae; Cypovirus X X Lymantria dispar Riboviria; Reoviridae; Spinareovirinae; Cypovirus; cypovirus 1 Cypovirus 1 Lymantria dispar Riboviria; Reoviridae; Spinareovirinae; Cypovirus; cypovirus 14 Cypovirus 14 Trichoplusia ni Riboviria; Reoviridae; Spinareovirinae; Cypovirus; cypovirus 15 Cypovirus 15 Choristoneura occiden- Riboviria; Reoviridae; Spinareovirinae; Cypovirus; talis cypovirus 16 Cypovirus 16 Riboviria; Reoviridae; Spinareovirinae; Cypovirus; Inachis io cypovirus 2 X X X X Cypovirus 2 Riboviria; Reoviridae; Spinareovirinae; Cypovirus; Daphnis nerii cypovirus unclassified Cypovirus Dendrolimus punctatus Riboviria; Reoviridae; Spinareovirinae; Cypovirus; cypovirus 22 unclassified Cypovirus Operophtera brumata Riboviria; Reoviridae; unclassified Reoviridae reovirus Una virus Riboviria; Togaviridae; Alphavirus Bombyx mori latent Riboviria; Tymovirales; Tymoviridae virus Bombyx mori Macula- Riboviria; Tymovirales; Tymoviridae; Maculavirus; like virus unclassified Maculavirus Abisko virus Riboviria; unclassified Riboviria X X Riboviria; unclassified Riboviria; unclassified RNA Hubei lepidoptera virus 2 viruses ShiM-2016 Riboviria; unclassified Riboviria; unclassified RNA Hubei lepidoptera virus 3 X X X X X X X X viruses ShiM-2016 Hubei sobemo-like virus Riboviria; unclassified Riboviria; unclassified RNA 33 viruses ShiM-2016 Bombyx mori cypovirus Satellites; RNA satellites; Double stranded RNA satel- X 1 satellite RNA lites; unclassified Double-stranded satellite RNAs

DISCUSSION

In order to better understand how V. cardui copes with infection, this study was undertaken with the aim of characterizing the various components of the V. cardui immune system to generate a reference for further immunological studies utilizing RT- qPCR and other molecular biological techniques. We successfully characterized over

1,000 gene transcripts related to the immune response and 357 gene transcripts with direct annotations to immune genes. Our experiment revealed many changes in gene regulation of V. cardui when presented with pathogen challenges, including the differential expression of a small amount of gene transcripts involved in the innate immune response. Much of the transcriptional response to pathogenic challenge in V. cardui was related to utilization of energy and partitioning of resources to fight the infection while still maintaining a developmental track to adulthood and homeostasis.

Previous studies such as, Tan et al. (2019), showed a limited number of differentially expressed gene transcripts involved in the immune response of the monarch butterfly

(Danaus plexippus) to challenge by the protozoan parasite, Ophryocystis elektroscirrha, which is in agreement with our findings presented here.

Interpretations of infection experiments were confounded by the detection of viral sequences within the V. cardui sequencing reads; including a closely related virus of

JcDNV, Mythimna loreyi densovirus (MlDNV), which was found in every replicate of each sample group except for replicate 1 of the non-infected group (Table 6). These sequences could possibly be endogenous viral elements (EVEs) within the genome, but further investigation is needed to affirm this assertion. Ophinni et al. (2019), provide a mechanism for which viral elements can be integrated into eukaryotic genomes and also 53 suggest the functionality of EVEs is to provide a form of specific immunity similar to the transgenerational antiviral immunity that endogenous retroviruses (ERVs) can have for mammals. This action of EVEs as a source of immune defense is easily paralleled in invertebrate animals, since the molecular machinery needed for the RNAi process remains consistent across all eukaryotic organisms. Ophinni et al. (2019) also discuss that this phenomenon has not been well-characterized in the lab, so caution must be applied when making conclusions as to where such antiviral immunity may originate from.

Recent work by Liu et al. (2020), suggests that EVE integration into invertebrate genomes may be a more common occurrence than previously thought, as prior research suggests integration of EVEs into the genomes of vertebrates, invertebrates, fungi, and plants (Horie et al., 2010; Horie and Tomonaga, 2011; Katzourakis and Gifford, 2010;

Gilbert et al., 2014). The integration of EVEs into the genome of non-model species presents a challenge when working with an organism that has not been extensively characterized in the lab and does not have the essential bioinformatic tools to tease apart instances of integration versus active infection. However, long read sequencing technologies could be used to discern the insertion of such elements into the genome.

Other molecular biological techniques, such as plaque assays, antibodies against viruses of interest, or in situ hybridization assays etc. may be performed to shed light on the infection status of insects used in immunological studies. Future studies should rear lab populations from field collected insects for several generations. This is an excellent starting point for such studies despite the fact that some lepidopteran specific baculoviruses and densoviruses have been shown to be vertically transmitted (Vitro et al.,

2014; Xu et al., 2014). While virus infections may or may not persist through vertical or 54 horizontal transmission, insects will at least have a uniform infection background to test from and reduced viral load after being reared in highly controlled environments. Another way to handle these low-level infections, would be to decrease the sequencing depth and increase the individual sequenced. This would keep the cost of sequencing down, while increasing the power of the analysis by adding additional replications.

Our first hypothesis was supported when examining our infected groups vs. control groups. We saw up-regulation of one transcript with immune related function in both the viral and bacterial groups versus their corresponding controls. In the bacterial versus control comparison, homeodomain interacting protein kinase (HIPK), was up- regulated. This particular protein is involved in the modulation of signaling pathways including Wnt/Wingless, Hippo, Notch, and JNK (Wang et al., 2020), of which, Hippo

(Hong et al., 2018) and JNK (Tafesh-Edwards & Eleftherianos, 2020) are highly involved in immune regulation. Within the viral versus control comparison, ras-related C3 botulinum toxin substrate 1 (Rac1), was up-regulated. This protein is known to be involved in the activation of hemocytes and subsequent initiation of the encapsulation of invading pathogens, as well as the activation of the phenol oxidase cascade leading to melanization of invading pathogens (Williams et al., 2006). Our second hypothesis was also partially supported, as seen in Supplementary Table 3, where the DE profiles of the bacterial versus control and viral versus control comparisons differ greatly, with the viral profile having more than 7x the amount of DE genes than the bacterial profile. However, we did not detect differences relative to immune function, which may have resulted from the aforementioned discovery of a myriad of lepidopteran viral pathogens within each sample group or may also indicate a lack of immune specificity for unique pathogen taxa. 55

Although only a few transcripts with an immune-related function were detected during the DE analysis, our results remain consistent with previous studies, as mentioned previously. Our results here also provoke questions into the infection status of the organisms used in previous studies, which may or may not present another obstacle that must be handled before any study of lepidopteran immunity may be undertaken and quantified with any amount of appreciable accuracy.

Challenges experienced in differential expression analyses aside, we were able to generate a nearly complete transcriptome containing 95.4% of all lepidopteran BUSCOs.

We were also able to characterize nearly 400 directly annotated immunity genes and over

700 more genes involved in immune-related functions. Our assembly will prove valuable to further validation studies utilizing RT-qPCR and other techniques for expression of transcripts involved in not only immune regulation, but regulation of other biological, cellular, and molecular processes.

CONCLUSION

We have assembled a transcriptome that will serve as a valuable reference to researchers studying lepidopteran immunity. Immune gene expression (in the transcriptome) was not strongly tied to infection status, but this is similar to other studies using similar approaches (Tan et al., 2019); possibly dynamics than immune gene transcript expression, such as a change in energy utilization, play a greater role in managing infection. We also revealed that infection experiments on non-model organisms may be operating in a more complex background than previously appreciated, with a mosaic of low-level infections persisting in apparent “healthy” populations. Although challenges in analysis of differentially expressed gene transcripts related to immune processes was encountered, our efforts have produced a valuable tool for future studies to utilize when exploring the immune response in V. cardui and other lepidopterans, as well as, recommendations that could improve future studies of immunity in other non-model organisms.

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APPENDIX

LIST OF SUPPLEMENTARY TABLES

Supplementary Table S1. Full set of predicted immunity genes utilizing previously annotated immune genes and GO annotations to identify transcripts associated with immune processes ...... 64

Supplementary Table S2. Antimicrobial peptide transcripts found during EnTAP annotation against APD3 database ...... 138

Supplementary Table S3. Full set of differentially expressed transcripts for each comparison ...... 140

Supplementary Table S1. Full set of predicted immunity genes utilizing previously annotated immune genes and GO annotations to identify transcripts associated with immune processes.

Query Sequence Subject Sequence Description GO Seed Ortholog tr|A0A5E4QI20|A0A5E4QI20_9NE OP Tafazzin family protein TRINITY_DN9994_c0_g1_i tr|A0A5E4QI20|A0A5E4QI20_9NE OS=Leptidea sinapis OX=189913 34740.HMEL004628-PA 1.p1 OP GN=LSINAPIS_LOCUS9120 PE=3 SV=1 TRINITY_DN99904_c0_g1 RVE54774.1 hypothetical protein RVE54774.1 13037.EHJ70574 _i1.p1 evm_000541 [Chilo suppressalis] tr|A0A2A4JKX4|A0A2A4JKX4_H TRINITY_DN9938_c0_g2_i tr|A0A2A4JKX4|A0A2A4JKX4_H ELVI Exocyst complex component 13037.EHJ70385 5.p1 ELVI 2 OS=Heliothis virescens OX=7102 GN=B5V51_830 PE=3 SV=1 tr|I4DNB1|I4DNB1_PAPXU Jun- TRINITY_DN9924_c0_g1_i related antigen OS=Papilio xuthus tr|I4DNB1|I4DNB1_PAPXU 34740.HMEL011777-PA 1.p1 OX=66420 GN=RR46_05794 PE=2 SV=1 tr|S4PCV3|S4PCV3_9NEOP TRINITY_DN9917_c0_g2_i tr|S4PCV3|S4PCV3_9NEOP Integrin beta subunit 1 OS=Pararge 34740.HMEL005744-PA 5.p1 aegeria OX=116150 PE=4 SV=1 UniRef90_A0A1B6IMF0 Integrin beta (Fragment) n=2 TRINITY_DN9917_c0_g1_i UniRef90_A0A1B6IMF0 Tax=Homalodisca liturata 34740.HMEL005744-PA 3.p1 TaxID=320908 RepID=A0A1B6IMF0_9HEMI XP_026492020.1 phenoloxidase- TRINITY_DN9916_c0_g1_i XP_026492020.1 activating enzyme-like [Vanessa 13037.EHJ73644 1.p1 tameamea] XP_032619857.1 plasminogen TRINITY_DN98944_c0_g1 61853.ENSNLEP0000000 XP_032619857.1 activator inhibitor 1 [Chelonoidis _i1.p1 9991 abingdonii] TRINITY_DN9791_c0_g1_i XP_026499044.1 DNA replication XP_026499044.1 13037.EHJ65989 2.p1 factor Cdt1 [Vanessa tameamea] tr|A0A194QND0|A0A194QND0_P APMA Transmembrane 9 TRINITY_DN9678_c0_g1_i tr|A0A194QND0|A0A194QND0_P superfamily member OS=Papilio 34740.HMEL012460-PA 1.p1 APMA machaon OX=76193 GN=RR48_14219 PE=3 SV=1 XP_022130726.1 peptidoglycan TRINITY_DN966_c0_g1_i4 XP_022130726.1 recognition protein-like [Pieris 34740.HMEL006763-PA .p1 rapae] tr|A0A2A4J6D0|A0A2A4J6D0_HE LVI Cyclin N-terminal domain- TRINITY_DN9631_c0_g1_i tr|A0A2A4J6D0|A0A2A4J6D0_HE containing protein OS=Heliothis 34740.HMEL007702-PA 2.p1 LVI virescens OX=7102 GN=B5V51_6973 PE=3 SV=1 tr|A0A1Z2TIV3|A0A1Z2TIV3_SP TRINITY_DN9625_c0_g1_i tr|A0A1Z2TIV3|A0A1Z2TIV3_SP OFR Autophagy related protein 8 34740.HMEL006641-PA 1.p1 OFR OS=Spodoptera frugiperda OX=7108 GN=atg8 PE=2 SV=1 tr|S4PEM6|S4PEM6_9NEOP Acyl- TRINITY_DN9500_c0_g2_i CoA Delta(11) desaturase tr|S4PEM6|S4PEM6_9NEOP 34740.HMEL012052-PA 12.p2 OS=Pararge aegeria OX=116150 PE=3 SV=1 65

UniRef90_A0A1B6DG41 Ig-like domain-containing protein TRINITY_DN9473_c0_g2_i UniRef90_A0A1B6DG41 (Fragment) n=1 Tax=Clastoptera 13037.EHJ77866 7.p1 arizonana TaxID=38151 RepID=A0A1B6DG41_9HEMI UniRef90_A0A482WYE9 Ig-like domain-containing protein n=1 TRINITY_DN9473_c0_g2_i UniRef90_A0A482WYE9 Tax=Laodelphax striatellus 7070.TC012539-PA 61.p1 TaxID=195883 RepID=A0A482WYE9_LAOST tr|A0A0S7IIK6|A0A0S7IIK6_9TEL E DSCAM (Fragment) TRINITY_DN9473_c0_g2_i tr|A0A0S7IIK6|A0A0S7IIK6_9TEL OS=Poeciliopsis prolifica 7070.TC012539-PA 17.p1 E OX=188132 GN=DSCAM PE=4 SV=1 tr|A0A212ELZ9|A0A212ELZ9_DA NPL Putative programmed cell TRINITY_DN9456_c0_g2_i tr|A0A212ELZ9|A0A212ELZ9_DA death 6-interacting protein 13037.EHJ65230 62.p1 NPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_210539 PE=4 SV=1 tr|A0A212EHU1|A0A212EHU1_D TRINITY_DN9332_c0_g1_i tr|A0A212EHU1|A0A212EHU1_D ANPL Defensin OS=Danaus 13037.EHJ63539 1.p2 ANPL plexippus plexippus OX=278856 GN=KGM_211227 PE=4 SV=1 tr|A0A212F1S0|A0A212F1S0_DA NPL LIM homeobox protein TRINITY_DN9238_c0_g1_i tr|A0A212F1S0|A0A212F1S0_DA (Fragment) OS=Danaus plexippus 13037.EHJ71081 2.p1 NPL plexippus OX=278856 GN=KGM_206411B PE=4 SV=1 tr|A0A212FBX3|A0A212FBX3_D ANPL PH-interacting protein TRINITY_DN9225_c0_g1_i tr|A0A212FBX3|A0A212FBX3_D OS=Danaus plexippus plexippus 13037.EHJ74876 2.p1 ANPL OX=278856 GN=KGM_214456 PE=4 SV=1 tr|A0A194PSZ7|A0A194PSZ7_PAP XU MAP kinase-activated protein TRINITY_DN9220_c0_g1_i tr|A0A194PSZ7|A0A194PSZ7_PAP 7091.BGIBMGA011190- kinase 2 OS=Papilio xuthus 2.p1 XU TA OX=66420 GN=RR46_12467 PE=3 SV=1 XP_026488314.1 hepatocyte TRINITY_DN9200_c2_g2_i growth factor-regulated tyrosine XP_026488314.1 13037.EHJ77606 1.p1 kinase substrate isoform X1 [Vanessa tameamea] XP_026492945.1 inhibitor of TRINITY_DN9154_c0_g1_i XP_026492945.1 nuclear factor kappa-B kinase 34740.HMEL007884-PA 1.p1 subunit alpha [Vanessa tameamea] XP_026489286.1 signal transducing TRINITY_DN9137_c1_g1_i XP_026489286.1 adapter molecule 1, partial [Vanessa 13037.EHJ69303 1.p1 tameamea] tr|A0A194R7A1|A0A194R7A1_PA TRINITY_DN9055_c0_g1_i tr|A0A194R7A1|A0A194R7A1_PA PMA Serpin B10 OS=Papilio 13037.EHJ65045 7.p1 PMA machaon OX=76193 GN=RR48_10716 PE=3 SV=1 tr|A0A194PHS7|A0A194PHS7_PA PXU STE20-related kinase adapter TRINITY_DN9000_c0_g1_i tr|A0A194PHS7|A0A194PHS7_PA protein alpha OS=Papilio xuthus 34740.HMEL009275-PA 15.p1 PXU OX=66420 GN=RR46_14192 PE=4 SV=1 66

tr|A0A212EIQ3|A0A212EIQ3_DA NPL Serpin 1 (Fragment) TRINITY_DN8_c27_g1_i3. tr|A0A212EIQ3|A0A212EIQ3_DA OS=Danaus plexippus plexippus 13037.EHJ63957 p1 NPL OX=278856 GN=KGM_202455B PE=3 SV=1 tr|S4PKY1|S4PKY1_9NEOP CLIP TRINITY_DN8912_c0_g1_i domain-containing serine protease tr|S4PKY1|S4PKY1_9NEOP 13037.EHJ73832 2.p1 OS=Pararge aegeria OX=116150 PE=3 SV=1 tr|A0A194PSD7|A0A194PSD7_PA TRINITY_DN8775_c0_g1_i tr|A0A194PSD7|A0A194PSD7_PA PXU Cullin-2 OS=Papilio xuthus 13037.EHJ66965 1.p1 PXU OX=66420 GN=RR46_12381 PE=3 SV=1 tr|A0A2H1VCA1|A0A2H1VCA1_S POFR SFRICE_003854 TRINITY_DN8756_c0_g2_i tr|A0A2H1VCA1|A0A2H1VCA1_S OS=Spodoptera frugiperda 13037.EHJ70644 8.p1 POFR OX=7108 GN=SFRICE_003854 PE=4 SV=1 tr|A0A5E4Q1P6|A0A5E4Q1P6_9N EOP TGc domain-containing TRINITY_DN8684_c0_g1_i tr|A0A5E4Q1P6|A0A5E4Q1P6_9N protein OS=Leptidea sinapis 34740.HMEL012424-PA 1.p1 EOP OX=189913 GN=LSINAPIS_LOCUS4690 PE=4 SV=1 XP_026494071.1 serine/threonine- TRINITY_DN863_c0_g1_i4 XP_026494071.1 protein kinase WNK1-like [Vanessa 34740.HMEL013929-PA .p1 tameamea] tr|A0A212FMJ9|A0A212FMJ9_DA NPL Serine/threonine protein kinase TRINITY_DN8606_c2_g1_i tr|A0A212FMJ9|A0A212FMJ9_DA OS=Danaus plexippus plexippus 13037.EHJ79112 1.p1 NPL OX=278856 GN=KGM_207034 PE=4 SV=1 tr|A0A346RAG2|A0A346RAG2_9 TRINITY_DN8588_c0_g1_i tr|A0A346RAG2|A0A346RAG2_9 NEOP Toll protein 8 7091.BGIBMGA011085- 1.p1 NEOP OS=Leguminivora glycinivorella TA OX=1035111 PE=2 SV=1 tr|A0A2W1BFM2|A0A2W1BFM2_ HELAM Ribosomal_L7Ae domain- TRINITY_DN8551_c0_g2_i tr|A0A2W1BFM2|A0A2W1BFM2_ containing protein OS=Helicoverpa 34740.HMEL013651-PA 4.p2 HELAM armigera OX=29058 GN=HaOG211118 PE=4 SV=1 TRINITY_DN8508_c0_g1_i 7091.BGIBMGA011471-

9.p1 TA tr|A0A2H1WJF9|A0A2H1WJF9_S POFR SFRICE_031199 (Fragment) TRINITY_DN8506_c0_g1_i tr|A0A2H1WJF9|A0A2H1WJF9_S OS=Spodoptera frugiperda 13037.EHJ73221 1.p1 POFR OX=7108 GN=SFRICE_031199 PE=4 SV=1 XP_026499957.1 translin- TRINITY_DN8496_c0_g1_i XP_026499957.1 associated protein X [Vanessa 13037.EHJ78868 1.p1 tameamea] XP_011556426.1 PREDICTED: E3 TRINITY_DN830_c5_g1_i1 XP_011556426.1 ubiquitin-protein ligase MYLIP- 34740.HMEL017980-PA .p1 like, partial [Plutella xylostella] tr|S4NY94|S4NY94_9NEOP TRINITY_DN8309_c0_g1_i tr|S4NY94|S4NY94_9NEOP Autophagy protein 5 OS=Pararge 13037.EHJ70753 14.p2 aegeria OX=116150 PE=3 SV=1 67

tr|A0A5E4Q240|A0A5E4Q240_9N EOP TEA domain-containing TRINITY_DN827_c0_g1_i1 tr|A0A5E4Q240|A0A5E4Q240_9N protein (Fragment) OS=Leptidea 13037.EHJ63836 1.p1 EOP sinapis OX=189913 GN=LSINAPIS_LOCUS4336 PE=4 SV=1 tr|F5HMI6|F5HMI6_ANOGA TRINITY_DN8256_c0_g1_i AGAP013145-PC OS=Anopheles tr|F5HMI6|F5HMI6_ANOGA 7165.AGAP013145-PB 17.p1 gambiae OX=7165 GN=11176012 PE=4 SV=1 XP_026733892.1 LOW QUALITY TRINITY_DN8256_c0_g1_i XP_026733892.1 PROTEIN: protein couch potato 13037.EHJ67566 1.p1 [Trichoplusia ni] XP_026492855.1 uncharacterized TRINITY_DN8211_c0_g1_i XP_026492855.1 protein LOC113398352 [Vanessa 13037.EHJ70909 1.p1 tameamea] XP_026486149.1 probable cyclin- TRINITY_DN8195_c0_g1_i dependent serine/threonine-protein XP_026486149.1 34740.HMEL006020-PA 3.p1 kinase DDB_G0292550 [Vanessa tameamea] XP_026494876.1 kelch domain- TRINITY_DN8185_c0_g1_i XP_026494876.1 containing protein 10 homolog 34740.HMEL007725-PA 1.p1 [Vanessa tameamea] tr|A0A2A4JKF0|A0A2A4JKF0_HE TRINITY_DN8148_c2_g1_i tr|A0A2A4JKF0|A0A2A4JKF0_HE LVI Uncharacterized protein 7091.BGIBMGA000603- 1.p1 LVI OS=Heliothis virescens OX=7102 TA GN=B5V51_1185 PE=4 SV=1 tr|A0A212FL83|A0A212FL83_DA NPL Autophagy-related protein 101 TRINITY_DN8057_c0_g1_i tr|A0A212FL83|A0A212FL83_DA isoform 1 OS=Danaus plexippus 13037.EHJ78617 12.p2 NPL plexippus OX=278856 GN=KGM_207822 PE=4 SV=1 XP_026496157.1 tyrosine-protein TRINITY_DN8026_c0_g1_i phosphatase non-receptor type 61F- XP_026496157.1 13037.EHJ74046 2.p1 like isoform X1 [Vanessa tameamea] TRINITY_DN798_c0_g1_i2 XP_026501296.1 phenoloxidase XP_026501296.1 34740.HMEL008342-PA .p1 subunit 2-like [Vanessa tameamea] tr|A0A194Q8L2|A0A194Q8L2_PA TRINITY_DN7978_c0_g1_i tr|A0A194Q8L2|A0A194Q8L2_PA PXU Toll-like receptor 4 13037.EHJ74227 2.p1 PXU OS=Papilio xuthus OX=66420 GN=RR46_04719 PE=4 SV=1 UniRef90_A0A2G9HCK7 Ferredoxin--NADP(+) reductase TRINITY_DN79618_c0_g1 4113.PGSC0003DMT400 UniRef90_A0A2G9HCK7 n=1 Tax=Handroanthus _i1.p1 009192 impetiginosus TaxID=429701 RepID=A0A2G9HCK7_9LAMI XP_022128445.1 bromodomain and TRINITY_DN7951_c0_g2_i XP_022128445.1 WD repeat-containing protein 3 13037.EHJ74876 1.p1 isoform X3 [Pieris rapae] TRINITY_DN7863_c0_g1_i XP_026494628.1 titin homolog XP_026494628.1 34740.HMEL002790-PA 13.p1 isoform X3 [Vanessa tameamea] XP_027043600.1 PHD finger TRINITY_DN7863_c0_g1_i XP_027043600.1 protein 10-like [Pocillopora 13037.EHJ68865 1.p1 damicornis] TRINITY_DN7819_c0_g1_i XP_026496542.1 cactin [Vanessa XP_026496542.1 34740.HMEL006752-PA 3.p1 tameamea] 68

tr|A0A3S2PDF4|A0A3S2PDF4_CH ISP APC1_C domain-containing TRINITY_DN7801_c0_g1_i tr|A0A3S2PDF4|A0A3S2PDF4_CH protein OS=Chilo suppressalis 34740.HMEL012998-PA 1.p1 ISP OX=168631 GN=evm_007155 PE=4 SV=1 XP_026486419.1 endoribonuclease TRINITY_DN8567_c0_g1_i XP_026486419.1 Dcr-1-like, partial [Vanessa 34740.HMEL014411-PA 1.p1 tameamea] tr|A0A212F0F7|A0A212F0F7_DA TRINITY_DN7787_c0_g1_i tr|A0A212F0F7|A0A212F0F7_DA NPL Toll receptor OS=Danaus 34740.HMEL010927-PA 1.p1 NPL plexippus plexippus OX=278856 GN=KGM_211472 PE=4 SV=1 TRINITY_DN7771_c0_g3_i 34740.HMEL016683-PA 1.p1 TRINITY_DN7771_c0_g2_i KPJ17196.1 Tyrosine-protein kinase KPJ17196.1 13037.EHJ66028 8.p2 Btk29A [Papilio machaon] tr|A0A212ENW7|A0A212ENW7_ DANPL Tyrosine-protein kinase TRINITY_DN7771_c0_g1_i tr|A0A212ENW7|A0A212ENW7_ OS=Danaus plexippus plexippus 13037.EHJ66028 2.p1 DANPL OX=278856 GN=KGM_207465 PE=3 SV=1 tr|A0A2H1WHJ0|A0A2H1WHJ0_S POFR FK506-binding protein TRINITY_DN7760_c0_g1_i tr|A0A2H1WHJ0|A0A2H1WHJ0_S OS=Spodoptera frugiperda 13037.EHJ72625 3.p1 POFR OX=7108 GN=SFRICE_004554 PE=3 SV=1 TRINITY_DN7744_c0_g1_i 34740.HMEL008488-PA 4.p1 tr|A0A0L7K447|A0A0L7K447_9N EOP Uncharacterized protein TRINITY_DN77122_c0_g1 tr|A0A0L7K447|A0A0L7K447_9N OS=Operophtera brumata 34740.HMEL013671-PA _i1.p1 EOP OX=104452 GN=OBRU01_25875 PE=4 SV=1 tr|A0A0L7LIV9|A0A0L7LIV9_9N EOP Protein bicaudal D TRINITY_DN7658_c0_g1_i tr|A0A0L7LIV9|A0A0L7LIV9_9N OS=Operophtera brumata 34740.HMEL013410-PA 7.p3 EOP OX=104452 GN=OBRU01_04945 PE=4 SV=1 tr|A0A2H1WVA7|A0A2H1WVA7_ SPOFR SFRICE_010499 TRINITY_DN7652_c0_g1_i tr|A0A2H1WVA7|A0A2H1WVA7_ 7091.BGIBMGA002790- OS=Spodoptera frugiperda 5.p1 SPOFR TA OX=7108 GN=SFRICE_010499 PE=4 SV=1 XP_026501495.1 uncharacterized TRINITY_DN7644_c0_g1_i XP_026501495.1 protein LOC113404726 isoform X3 34740.HMEL008635-PA 2.p1 [Vanessa tameamea] TRINITY_DN763_c0_g1_i1 13037.EHJ76914 3.p1 TRINITY_DN762_c0_g2_i1 XP_026496066.1 sorting nexin-30- XP_026496066.1 13037.EHJ75969 .p1 like [Vanessa tameamea] TRINITY_DN762_c0_g1_i1 XP_032513408.1 hemocytin XP_032513408.1 13037.EHJ75969 2.p1 [Danaus plexippus plexippus] TRINITY_DN7619_c0_g2_i 13037.EHJ71973 1.p1 OWR41866.1 E3 ubiquitin-protein TRINITY_DN7559_c0_g1_i OWR41866.1 ligase mind-bomb, partial [Danaus 13037.EHJ64505 6.p1 plexippus plexippus] 69

tr|A0A212ENA9|A0A212ENA9_D TRINITY_DN7523_c0_g1_i tr|A0A212ENA9|A0A212ENA9_D ANPL LST8 protein OS=Danaus 13037.EHJ65808 1.p1 ANPL plexippus plexippus OX=278856 GN=KGM_207727 PE=4 SV=1 UniRef90_S4PLJ8 Anaphase- TRINITY_DN7494_c0_g2_i promoting complex subunit 5 n=1 UniRef90_S4PLJ8 13037.EHJ67970 1.p2 Tax=Pararge aegeria TaxID=116150 RepID=S4PLJ8_9NEOP XP_026500664.1 nuclear pore TRINITY_DN7382_c0_g1_i XP_026500664.1 complex protein DDB_G0274915- 13037.EHJ64614 1.p1 like [Vanessa tameamea] tr|A0A194QQJ4|A0A194QQJ4_PA PMA SNF-related serine/threonine- TRINITY_DN7695_c0_g1_i tr|A0A194QQJ4|A0A194QQJ4_PA protein kinase OS=Papilio machaon 34740.HMEL013682-PA 6.p1 PMA OX=76193 GN=RR48_07656 PE=3 SV=1 tr|A0A2H1X4U0|A0A2H1X4U0_S POFR Argonaute 1-PCa TRINITY_DN7315_c0_g1_i tr|A0A2H1X4U0|A0A2H1X4U0_S 7091.BGIBMGA003670- OS=Spodoptera frugiperda 5.p1 POFR TA OX=7108 GN=SFRICE_000078 PE=2 SV=1 tr|A0A2H1X4U0|A0A2H1X4U0_S POFR Argonaute 1-PCa TRINITY_DN7315_c0_g1_i tr|A0A2H1X4U0|A0A2H1X4U0_S 7091.BGIBMGA003670- OS=Spodoptera frugiperda 1.p1 POFR TA OX=7108 GN=SFRICE_000078 PE=2 SV=1 tr|A0A194RAV4|A0A194RAV4_PA PMA Apoptotic protease-activating TRINITY_DN7275_c0_g1_i tr|A0A194RAV4|A0A194RAV4_PA factor 1 OS=Papilio machaon 13037.EHJ68576 10.p1 PMA OX=76193 GN=RR48_13486 PE=4 SV=1 XP_026499148.1 protein TRINITY_DN7249_c0_g2_i XP_026499148.1 ecdysoneless homolog [Vanessa 34740.HMEL006646-PA 3.p1 tameamea] tr|Q76FN3|Q76FN3_BOMMO G TRINITY_DN7361_c0_g2_i protein alpha q isoform 1 7091.BGIBMGA012492- tr|Q76FN3|Q76FN3_BOMMO 2.p1 OS=Bombyx mori OX=7091 TA GN=Gq PE=2 SV=1 TRINITY_DN7215_c0_g1_i 34740.HMEL007340-PA 6.p1 tr|A0A194PLT3|A0A194PLT3_PAP TRINITY_DN7191_c0_g2_i tr|A0A194PLT3|A0A194PLT3_PAP XU Tight junction protein ZO-2 7091.BGIBMGA010279- 10.p2 XU OS=Papilio xuthus OX=66420 TA GN=RR46_06738 PE=3 SV=1 tr|A0A194R5G4|A0A194R5G4_PA PMA Vacuolar protein sorting- TRINITY_DN7188_c0_g2_i tr|A0A194R5G4|A0A194R5G4_PA associated protein 16 homolog 34740.HMEL007914-PA 3.p1 PMA OS=Papilio machaon OX=76193 GN=RR48_10547 PE=3 SV=1 tr|A0A212F7Q2|A0A212F7Q2_DA NPL Uncharacterized protein TRINITY_DN7176_c0_g1_i tr|A0A212F7Q2|A0A212F7Q2_DA OS=Danaus plexippus plexippus 13037.EHJ73283 11.p2 NPL OX=278856 GN=KGM_211832 PE=4 SV=1 XP_026485297.1 autophagy-related TRINITY_DN7159_c0_g1_i XP_026485297.1 protein 16-1 isoform X1 [Vanessa 13037.EHJ73902 6.p1 tameamea] 70

tr|A0A194PZJ0|A0A194PZJ0_PAP TRINITY_DN7744_c0_g1_i tr|A0A194PZJ0|A0A194PZJ0_PAP XU Ephrin-B2a OS=Papilio xuthus 34740.HMEL008488-PA 1.p1 XU OX=66420 GN=RR46_12610 PE=3 SV=1 tr|A0A437BHZ9|A0A437BHZ9_C HISP NTF2 domain-containing TRINITY_DN7090_c2_g1_i tr|A0A437BHZ9|A0A437BHZ9_C protein OS=Chilo suppressalis 13037.EHJ63410 1.p2 HISP OX=168631 GN=evm_005247 PE=4 SV=1 XP_026489939.1 plasminogen TRINITY_DN7074_c1_g1_i XP_026489939.1 activator inhibitor 1 RNA-binding 13037.EHJ75994 10.p2 protein-like [Vanessa tameamea] XP_026487843.1 poly(U)-specific TRINITY_DN7060_c0_g1_i XP_026487843.1 endoribonuclease homolog [Vanessa 13037.EHJ66341 4.p1 tameamea] tr|A0A2H1VQP7|A0A2H1VQP7_S POFR SFRICE_003333 (Fragment) TRINITY_DN7049_c0_g1_i tr|A0A2H1VQP7|A0A2H1VQP7_S OS=Spodoptera frugiperda 13037.EHJ67216 2.p1 POFR OX=7108 GN=SFRICE_003333 PE=4 SV=1 XP_026489954.1 activated Cdc42 TRINITY_DN6904_c0_g1_i XP_026489954.1 kinase-like isoform X1 [Vanessa 34740.HMEL012077-PA 17.p1 tameamea] XP_026489981.1 activated Cdc42 TRINITY_DN6904_c0_g1_i XP_026489981.1 kinase-like isoform X4 [Vanessa 34740.HMEL012077-PA 16.p1 tameamea] XP_026489970.1 activated Cdc42 TRINITY_DN6904_c0_g1_i XP_026489970.1 kinase-like isoform X3 [Vanessa 34740.HMEL012077-PA 10.p1 tameamea] tr|A0A212FF76|A0A212FF76_DA NPL Tuberin like protein TRINITY_DN6895_c1_g2_i tr|A0A212FF76|A0A212FF76_DA OS=Danaus plexippus plexippus 13037.EHJ76090 12.p1 NPL OX=278856 GN=KGM_209357 PE=4 SV=1 tr|A0A212FF76|A0A212FF76_DA NPL Tuberin like protein TRINITY_DN6895_c1_g2_i tr|A0A212FF76|A0A212FF76_DA OS=Danaus plexippus plexippus 13037.EHJ76090 1.p1 NPL OX=278856 GN=KGM_209357 PE=4 SV=1 tr|A0A2A4IY45|A0A2A4IY45_HE LVI VPS9 domain-containing TRINITY_DN6860_c0_g1_i tr|A0A2A4IY45|A0A2A4IY45_HE protein OS=Heliothis virescens 34740.HMEL011987-PA 11.p1 LVI OX=7102 GN=B5V51_10467 PE=4 SV=1 XP_026500867.1 peptidoglycan- TRINITY_DN6855_c0_g1_i XP_026500867.1 recognition protein SA-like isoform 13037.EHJ70144 8.p1 X1 [Vanessa tameamea] XP_026500868.1 peptidoglycan- TRINITY_DN6855_c0_g1_i XP_026500868.1 recognition protein SA-like isoform 13037.EHJ70144 12.p1 X2 [Vanessa tameamea] XP_026491018.1 uncharacterized TRINITY_DN6849_c0_g1_i 7091.BGIBMGA004738- XP_026491018.1 protein LOC113397079 [Vanessa 1.p1 TA tameamea] XP_026493991.1 apoptosis- TRINITY_DN6822_c0_g1_i XP_026493991.1 stimulating of p53 protein 2 isoform 13037.EHJ73745 16.p1 X3 [Vanessa tameamea] 71

XP_026493991.1 apoptosis- TRINITY_DN6822_c0_g1_i XP_026493991.1 stimulating of p53 protein 2 isoform 13037.EHJ73745 12.p1 X3 [Vanessa tameamea] XP_026757235.1 enhancer of TRINITY_DN6779_c0_g1_i XP_026757235.1 mRNA-decapping protein 4-like 34740.HMEL012843-PA 5.p2 [Galleria mellonella] XP_026501273.1 histone-lysine N- TRINITY_DN6748_c0_g1_i XP_026501273.1 methyltransferase SETD1 isoform 13037.EHJ79119 1.p1 X2 [Vanessa tameamea] GBO42387.1 hypothetical protein TRINITY_DN6726_c1_g1_i GBO42387.1 AVEN_105667_1, partial [Araneus 13037.EHJ63159 3.p1 ventricosus] tr|A0A3S2PJ20|A0A3S2PJ20_CHI TRINITY_DN6726_c1_g1_i tr|A0A3S2PJ20|A0A3S2PJ20_CHI SP Uncharacterized protein 13037.EHJ63159 2.p1 SP OS=Chilo suppressalis OX=168631 GN=evm_002500 PE=4 SV=1 tr|S4P2Q1|S4P2Q1_9NEOP TRINITY_DN6726_c1_g1_i Uncharacterized protein (Fragment) tr|S4P2Q1|S4P2Q1_9NEOP 13037.EHJ63159 11.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 tr|A0A3S2PJ20|A0A3S2PJ20_CHI TRINITY_DN6726_c0_g1_i tr|A0A3S2PJ20|A0A3S2PJ20_CHI SP Uncharacterized protein 13037.EHJ63159 9.p1 SP OS=Chilo suppressalis OX=168631 GN=evm_002500 PE=4 SV=1 tr|H9J5D8|H9J5D8_BOMMO TRINITY_DN6726_c0_g1_i Uncharacterized protein tr|H9J5D8|H9J5D8_BOMMO 13037.EHJ63159 10.p1 OS=Bombyx mori OX=7091 PE=4 SV=1 XP_026491394.1 TNF receptor- TRINITY_DN6725_c0_g1_i XP_026491394.1 associated factor 4 [Vanessa 13037.EHJ67361 4.p1 tameamea] XP_026488064.1 ubiquitin TRINITY_DN6723_c1_g3_i XP_026488064.1 carboxyl-terminal hydrolase CYLD 34740.HMEL017223-PA 2.p1 isoform X1 [Vanessa tameamea] tr|A0A212EZX7|A0A212EZX7_D ANPL Dual specificity mitogen- TRINITY_DN6712_c0_g1_i tr|A0A212EZX7|A0A212EZX7_D activated protein kinase kinase 4 13037.EHJ70363 5.p2 ANPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_200381 PE=3 SV=1 KOB55722.1 hypothetical protein TRINITY_DN6658_c0_g2_i KOB55722.1 OBRU01_25875 [Operophtera 34740.HMEL013671-PA 1.p1 brumata] tr|A0A194QPN7|A0A194QPN7_PA PMA Facilitated trehalose TRINITY_DN6912_c0_g1_i tr|A0A194QPN7|A0A194QPN7_PA transporter Tret1 OS=Papilio 34740.HMEL013939-PA 5.p1 PMA machaon OX=76193 GN=RR48_08480 PE=3 SV=1 tr|A0A212F888|A0A212F888_DA NPL 40S ribosomal protein S6 TRINITY_DN6631_c0_g1_i tr|A0A212F888|A0A212F888_DA OS=Danaus plexippus plexippus 13037.EHJ73490 4.p1 NPL OX=278856 GN=KGM_208035 PE=3 SV=1 tr|T1HFP7|T1HFP7_RHOPR TRINITY_DN6631_c0_g1_i Uncharacterized protein (Fragment) tr|T1HFP7|T1HFP7_RHOPR 34740.HMEL004054-PA 2.p2 OS=Rhodnius prolixus OX=13249 PE=4 SV=1 72

tr|A0A212F888|A0A212F888_DA NPL 40S ribosomal protein S6 TRINITY_DN6631_c0_g1_i tr|A0A212F888|A0A212F888_DA OS=Danaus plexippus plexippus 13037.EHJ73490 2.p1 NPL OX=278856 GN=KGM_208035 PE=3 SV=1 TRINITY_DN658_c4_g1_i1 VDO79388.1 unnamed protein VDO79388.1 34740.HMEL002834-PA .p2 product [Soboliphyme baturini] XP_026488084.1 endoplasmic TRINITY_DN6577_c0_g1_i XP_026488084.1 reticulum resident protein 29 13037.EHJ79056 1.p1 [Vanessa tameamea] XP_026492125.1 multiple inositol TRINITY_DN65526_c0_g1 XP_026492125.1 polyphosphate phosphatase 1-like 13037.EHJ75790 _i1.p1 [Vanessa tameamea] TRINITY_DN6528_c0_g1_i XP_026486533.1 mucolipin-3-like XP_026486533.1 34740.HMEL013941-PA 1.p1 [Vanessa tameamea] tr|A0A2A4J0I0|A0A2A4J0I0_HEL TRINITY_DN6525_c0_g1_i tr|A0A2A4J0I0|A0A2A4J0I0_HEL VI Uncharacterized protein 7091.BGIBMGA002693- 1.p1 VI OS=Heliothis virescens OX=7102 TA GN=B5V51_9969 PE=4 SV=1 XP_026493107.1 dynein heavy TRINITY_DN650_c0_g1_i2 XP_026493107.1 chain, cytoplasmic [Vanessa 7176.CPIJ007950-PA .p1 tameamea] tr|A0A2W1BN54|A0A2W1BN54_ HELAM GATA-type domain- TRINITY_DN6503_c0_g1_i tr|A0A2W1BN54|A0A2W1BN54_ containing protein OS=Helicoverpa 34740.HMEL007455-PA 4.p1 HELAM armigera OX=29058 GN=HaOG204512 PE=4 SV=1 XP_026484748.1 GATA-binding TRINITY_DN6503_c0_g1_i XP_026484748.1 factor A-like isoform X3 [Vanessa 34740.HMEL007455-PA 12.p1 tameamea] tr|A0A194Q6H6|A0A194Q6H6_PA TRINITY_DN64_c1_g2_i1. tr|A0A194Q6H6|A0A194Q6H6_PA PXU LIM domain kinase 1 13037.EHJ79268 p1 PXU OS=Papilio xuthus OX=66420 GN=RR46_10323 PE=4 SV=1 XP_026497235.1 E3 ubiquitin- TRINITY_DN6488_c0_g1_i XP_026497235.1 protein ligase TRIM33 isoform X2 34740.HMEL010581-PA 1.p1 [Vanessa tameamea] TRINITY_DN6470_c0_g1_i XP_028042518.1 zonadhesin-like XP_028042518.1 13037.EHJ63159 8.p1 [Bombyx mandarina] tr|A0A2A4IW13|A0A2A4IW13_H ELVI Uncharacterized protein TRINITY_DN6470_c0_g1_i tr|A0A2A4IW13|A0A2A4IW13_H (Fragment) OS=Heliothis virescens 13037.EHJ63159 4.p1 ELVI OX=7102 GN=B5V51_11644 PE=4 SV=1 tr|S4P948|S4P948_9NEOP Bicaudal TRINITY_DN63732_c0_g1 tr|S4P948|S4P948_9NEOP C OS=Pararge aegeria OX=116150 13037.EHJ78372 _i1.p1 PE=4 SV=1 TRINITY_DN6365_c0_g1_i GBP31959.1 Ran-binding protein GBP31959.1 34740.HMEL010432-PA 2.p1 10 [Eumeta japonica] tr|A0A2A4K1Y2|A0A2A4K1Y2_H ELVI G_PROTEIN_RECEP_F1_2 TRINITY_DN6348_c0_g2_i tr|A0A2A4K1Y2|A0A2A4K1Y2_H domain-containing protein 34740.HMEL009221-PA 2.p1 ELVI OS=Heliothis virescens OX=7102 GN=B5V51_6356 PE=3 SV=1 73

tr|A0A2A4K1Y2|A0A2A4K1Y2_H ELVI G_PROTEIN_RECEP_F1_2 TRINITY_DN6348_c0_g1_i tr|A0A2A4K1Y2|A0A2A4K1Y2_H domain-containing protein 34740.HMEL009221-PA 1.p1 ELVI OS=Heliothis virescens OX=7102 GN=B5V51_6356 PE=3 SV=1 tr|A0A3S2M5F5|A0A3S2M5F5_C HISP Homeobox domain-containing TRINITY_DN63399_c0_g1 tr|A0A3S2M5F5|A0A3S2M5F5_C protein OS=Chilo suppressalis 34740.HMEL025054-PA _i2.p1 HISP OX=168631 GN=evm_003580 PE=3 SV=1 XP_026487065.1 mediator of RNA TRINITY_DN6328_c0_g1_i XP_026487065.1 polymerase II transcription subunit 34740.HMEL009027-PA 2.p2 27 [Vanessa tameamea] TRINITY_DN6322_c0_g1_i XP_026493986.1 kelch-like protein XP_026493986.1 34740.HMEL016437-PA 7.p1 10 [Vanessa tameamea] tr|A0A212F5R9|A0A212F5R9_DA NPL Suppressor of cytokine TRINITY_DN6311_c0_g2_i tr|A0A212F5R9|A0A212F5R9_DA signaling 6 OS=Danaus plexippus 13037.EHJ72563 1.p2 NPL plexippus OX=278856 GN=KGM_207274 PE=4 SV=1 XP_026489954.1 activated Cdc42 TRINITY_DN6904_c0_g2_i XP_026489954.1 kinase-like isoform X1 [Vanessa 34740.HMEL012077-PA 28.p1 tameamea] tr|O96383|O96383_HYPCU TRINITY_DN62_c0_g1_i20 Immune-related Hdd13 tr|O96383|O96383_HYPCU 34740.HMEL010004-PA .p1 OS=Hyphantria cunea OX=39466 PE=2 SV=1 tr|A0A482VFC0|A0A482VFC0_9C UCU Pkinase domain containing TRINITY_DN6297_c0_g1_i tr|A0A482VFC0|A0A482VFC0_9C protein OS=Asbolus verrucosus 34740.HMEL007938-PA 6.p1 UCU OX=1661398 GN=BDFB_005206 PE=3 SV=1 tr|A0A0L7LQH1|A0A0L7LQH1_9 NEOP DID protein TRINITY_DN6230_c0_g1_i tr|A0A0L7LQH1|A0A0L7LQH1_9 OS=Operophtera brumata 34740.HMEL005390-PA 10.p2 NEOP OX=104452 GN=OBRU01_03898 PE=4 SV=1 tr|A0A2H1WKU0|A0A2H1WKU0_ SPOFR SFRICE010188.2 TRINITY_DN6176_c0_g1_i tr|A0A2H1WKU0|A0A2H1WKU0_ OS=Spodoptera frugiperda 13037.EHJ76936 13.p1 SPOFR OX=7108 GN=SFRICE010188.2 PE=4 SV=1 XP_026498873.1 RNA TRINITY_DN6169_c1_g1_i pseudouridylate synthase domain- XP_026498873.1 13037.EHJ68106 1.p1 containing protein 1-like isoform X1 [Vanessa tameamea] UniRef90_A0A0N1IKJ1 TRINITY_DN6164_c0_g1_i Hemicentin-1 n=1 Tax=Papilio UniRef90_A0A0N1IKJ1 13037.EHJ72797 21.p1 machaon TaxID=76193 RepID=A0A0N1IKJ1_PAPMA tr|S4PM57|S4PM57_9NEOP TRINITY_DN6154_c0_g2_i tr|S4PM57|S4PM57_9NEOP Protein CLP1 homolog OS=Pararge 34740.HMEL004954-PA 6.p1 aegeria OX=116150 PE=3 SV=1 TRINITY_DN6053_c1_g1_i XP_026501164.1 protein vav XP_026501164.1 13037.EHJ74634 5.p1 isoform X2 [Vanessa tameamea] TRINITY_DN6053_c0_g1_i XP_012547899.1 protein vav XP_012547899.1 13037.EHJ74634 4.p1 isoform X3 [Bombyx mori] 74

tr|A0A437B2I2|A0A437B2I2_CHI TRINITY_DN8590_c0_g1_i tr|A0A437B2I2|A0A437B2I2_CHI SP Mitogen-activated protein kinase 7165.AGAP005898-PA 2.p1 SP OS=Chilo suppressalis OX=168631 GN=evm_010920 PE=3 SV=1 XP_026496952.1 uncharacterized TRINITY_DN603_c0_g1_i1 XP_026496952.1 protein LOC113401309 [Vanessa 13037.EHJ63782 7.p1 tameamea] tr|A0A2A4J6Y9|A0A2A4J6Y9_HE LVI BHLH domain-containing TRINITY_DN6031_c1_g1_i tr|A0A2A4J6Y9|A0A2A4J6Y9_HE protein OS=Heliothis virescens 13037.EHJ70086 14.p1 LVI OX=7102 GN=B5V51_6411 PE=4 SV=1 tr|A0A2W1BGL4|A0A2W1BGL4_ HELAM BHLH domain-containing TRINITY_DN6031_c1_g1_i tr|A0A2W1BGL4|A0A2W1BGL4_ protein OS=Helicoverpa armigera 13037.EHJ70086 1.p1 HELAM OX=29058 GN=HaOG208520 PE=4 SV=1 XP_026492021.1 phenoloxidase- TRINITY_DN6016_c0_g1_i XP_026492021.1 activating enzyme-like [Vanessa 34740.HMEL004371-PA 1.p1 tameamea] XP_026489592.1 G protein-coupled TRINITY_DN5_c16_g1_i1. 7091.BGIBMGA010747- XP_026489592.1 receptor kinase 2 isoform X2 p1 TA [Vanessa tameamea] TRINITY_DN6053_c1_g1_i XP_026501164.1 protein vav XP_026501164.1 13037.EHJ74634 4.p1 isoform X2 [Vanessa tameamea] XP_026499616.1 matrix TRINITY_DN5938_c0_g1_i XP_026499616.1 metalloproteinase-2-like [Vanessa 13037.EHJ69947 9.p1 tameamea] tr|A0A0L7LBI3|A0A0L7LBI3_9N EOP Anaphase-promoting complex TRINITY_DN7415_c0_g1_i tr|A0A0L7LBI3|A0A0L7LBI3_9N subunit 10 OS=Operophtera 34740.HMEL005234-PA 1.p2 EOP brumata OX=104452 GN=OBRU01_12037 PE=3 SV=1 tr|A0A212F6V5|A0A212F6V5_DA NPL MARVEL domain-containing TRINITY_DN9499_c0_g1_i tr|A0A212F6V5|A0A212F6V5_DA protein OS=Danaus plexippus 34740.HMEL012113-PA 1.p1 NPL plexippus OX=278856 GN=KGM_206086 PE=4 SV=1 tr|A0A2H1W267|A0A2H1W267_S POFR SFRICE_000275 TRINITY_DN5816_c3_g1_i tr|A0A2H1W267|A0A2H1W267_S OS=Spodoptera frugiperda 34740.HMEL009775-PA 1.p1 POFR OX=7108 GN=SFRICE_000275 PE=4 SV=1 XP_026487713.1 E3 ubiquitin- TRINITY_DN5812_c0_g1_i XP_026487713.1 protein ligase Su(dx) isoform X2 34740.HMEL006816-PA 29.p1 [Vanessa tameamea] tr|A0A3S2LP30|A0A3S2LP30_CHI SP Protein kinase domain- TRINITY_DN5795_c0_g2_i tr|A0A3S2LP30|A0A3S2LP30_CHI containing protein OS=Chilo 34740.HMEL017558-PA 1.p1 SP suppressalis OX=168631 GN=evm_004421 PE=4 SV=1 tr|A0A0E3IQG1|A0A0E3IQG1_SP TRINITY_DN5795_c0_g1_i tr|A0A0E3IQG1|A0A0E3IQG1_SP OLT Autophagy related protein 1 34740.HMEL017558-PA 8.p1 OLT isoform A OS=Spodoptera litura OX=69820 GN=Atg1 PE=2 SV=1 tr|A0A0E3IQG1|A0A0E3IQG1_SP TRINITY_DN5795_c0_g1_i tr|A0A0E3IQG1|A0A0E3IQG1_SP OLT Autophagy related protein 1 34740.HMEL017558-PA 1.p1 OLT isoform A OS=Spodoptera litura OX=69820 GN=Atg1 PE=2 SV=1 75

TRINITY_DN57812_c0_g1 VVD04014.1 unnamed protein VVD04014.1 34740.HMEL007941-PA _i2.p1 product [Leptidea sinapis] sp|Q9SU35|ERLL1_ARATH TRINITY_DN57730_c0_g1 pEARLI1-like lipid transfer protein sp|Q9SU35|ERLL1_ARATH 4155.Migut.E00848.1.p _i1.p1 1 OS=Arabidopsis thaliana OX=3702 GN=AZI1 PE=1 SV=1 tr|A0A3S2NKX6|A0A3S2NKX6_C HISP WD_REPEATS_REGION TRINITY_DN5719_c0_g2_i tr|A0A3S2NKX6|A0A3S2NKX6_C domain-containing protein 13037.EHJ65465 2.p1 HISP OS=Chilo suppressalis OX=168631 GN=evm_005042 PE=4 SV=1 tr|A0A3S2NKX6|A0A3S2NKX6_C HISP WD_REPEATS_REGION TRINITY_DN5719_c0_g1_i tr|A0A3S2NKX6|A0A3S2NKX6_C domain-containing protein 13037.EHJ65465 1.p1 HISP OS=Chilo suppressalis OX=168631 GN=evm_005042 PE=4 SV=1 UniRef90_A0A0A6Z973 TRINITY_DN56991_c1_g1 Calreticulin (Fragment) n=6 UniRef90_A0A0A6Z973 3702.AT1G56340.1 _i1.p2 Tax=Sonneratia TaxID=122811 RepID=A0A0A6Z973_9MYRT tr|Q19AA9|Q19AA9_BOMMO TRINITY_DN7827_c0_g1_i Transport protein Sec61 alpha 7091.BGIBMGA012687- tr|Q19AA9|Q19AA9_BOMMO 3.p1 subunit OS=Bombyx mori TA OX=7091 GN=733068 PE=2 SV=1 XP_026498695.1 enhancer of TRINITY_DN6485_c0_g1_i XP_026498695.1 mRNA-decapping protein 4-like, 34740.HMEL012843-PA 1.p1 partial [Vanessa tameamea] tr|A0A194Q977|A0A194Q977_PAP XU MAP kinase-activating death TRINITY_DN5637_c0_g1_i tr|A0A194Q977|A0A194Q977_PAP domain protein OS=Papilio xuthus 13037.EHJ72087 2.p2 XU OX=66420 GN=RR46_05178 PE=4 SV=1 tr|A0A437B6L4|A0A437B6L4_CH ISP UDENN domain-containing TRINITY_DN5637_c0_g1_i tr|A0A437B6L4|A0A437B6L4_CH protein OS=Chilo suppressalis 34740.HMEL014848-PA 2.p1 ISP OX=168631 GN=evm_009377 PE=4 SV=1 XP_026501192.1 dual specificity TRINITY_DN5620_c0_g1_i mitogen-activated protein kinase XP_026501192.1 13037.EHJ73699 2.p1 kinase 7-like isoform X2 [Vanessa tameamea] tr|A0A194PKZ8|A0A194PKZ8_PA PXU Ras-like GTP-binding protein TRINITY_DN6158_c0_g1_i tr|A0A194PKZ8|A0A194PKZ8_PA RhoL OS=Papilio xuthus 13037.EHJ66117 3.p1 PXU OX=66420 GN=RR46_10664 PE=4 SV=1 XP_026495038.1 nucleosome- TRINITY_DN5605_c1_g1_i XP_026495038.1 remodeling factor subunit 13037.EHJ73003 1.p1 NURF301 [Vanessa tameamea] UniRef90_A0A1B6GKD3 Protein kinase domain-containing protein TRINITY_DN55681_c0_g1 UniRef90_A0A1B6GKD3 (Fragment) n=1 Tax=Cuerna arida 7159.AAEL004351-PC _i2.p1 TaxID=1464854 RepID=A0A1B6GKD3_9HEMI tr|A0A437B9R7|A0A437B9R7_CH ISP Mitogen-activated protein TRINITY_DN19324_c0_g1 tr|A0A437B9R7|A0A437B9R7_CH kinase OS=Chilo suppressalis 13037.EHJ68412 _i2.p1 ISP OX=168631 GN=evm_008209 PE=4 SV=1 76

tr|A0A2W1BFM2|A0A2W1BFM2_ HELAM Ribosomal_L7Ae domain- TRINITY_DN8551_c0_g1_i tr|A0A2W1BFM2|A0A2W1BFM2_ containing protein OS=Helicoverpa 34740.HMEL013651-PA 1.p1 HELAM armigera OX=29058 GN=HaOG211118 PE=4 SV=1 XP_031763920.1 probable G- TRINITY_DN19170_c0_g1 XP_031763920.1 protein coupled receptor 158 13037.EHJ75867 _i4.p1 isoform X1 [Galleria mellonella] XP_031763921.1 probable G- TRINITY_DN19170_c0_g1 XP_031763921.1 protein coupled receptor 158 34740.HMEL012400-PA _i3.p1 isoform X2 [Galleria mellonella] tr|A0A2H1X0W9|A0A2H1X0W9_ SPOFR SFRICE_016723 TRINITY_DN19036_c0_g1 tr|A0A2H1X0W9|A0A2H1X0W9_ (Fragment) OS=Spodoptera 13037.EHJ72113 _i1.p1 SPOFR frugiperda OX=7108 GN=SFRICE_016723 PE=4 SV=1 XP_026486545.1 S-phase kinase- TRINITY_DN12045_c2_g2 XP_026486545.1 associated protein 2-like [Vanessa 34740.HMEL010736-PA _i6.p1 tameamea] XP_026486458.1 embryonic TRINITY_DN3865_c0_g1_i XP_026486458.1 polarity protein dorsal-like isoform 13037.EHJ69235 1.p1 X1 [Vanessa tameamea] tr|A0A212FMQ0|A0A212FMQ0_D ANPL Molybdopterin TRINITY_DN1222_c0_g1_i tr|A0A212FMQ0|A0A212FMQ0_D molybdenumtransferase 7091.BGIBMGA012855- 1.p1 ANPL OS=Danaus plexippus plexippus TA OX=278856 GN=KGM_206899 PE=3 SV=1 tr|A0A2A4JMZ3|A0A2A4JMZ3_H ELVI Protein kinase domain- TRINITY_DN1124_c4_g1_i tr|A0A2A4JMZ3|A0A2A4JMZ3_H containing protein OS=Heliothis 13037.EHJ70836 32.p1 ELVI virescens OX=7102 GN=B5V51_15063 PE=3 SV=1 tr|A0A212F458|A0A212F458_DA NPL Hemicentin-1 like protein TRINITY_DN19163_c0_g1 tr|A0A212F458|A0A212F458_DA OS=Danaus plexippus plexippus 13037.EHJ71932 _i2.p1 NPL OX=278856 GN=KGM_209682 PE=4 SV=1 TRINITY_DN1882_c0_g1_i XP_028173984.1 CLIP-associating XP_028173984.1 34740.HMEL015868-PA 3.p1 protein [Ostrinia furnacalis] tr|A0A212F643|A0A212F643_DA TRINITY_DN15144_c0_g1 tr|A0A212F643|A0A212F643_DA NPL Acinus OS=Danaus plexippus 34740.HMEL011367-PA _i1.p1 NPL plexippus OX=278856 GN=KGM_214130 PE=4 SV=1 tr|C9DTM4|C9DTM4_BOMMO TRINITY_DN1298_c0_g1_i 7091.BGIBMGA012467- tr|C9DTM4|C9DTM4_BOMMO CAP isoform A OS=Bombyx mori 17.p2 TA OX=7091 PE=2 SV=1 tr|I4DMS1|I4DMS1_PAPPL TRINITY_DN1855_c0_g1_i Eukaryotic translation initiation tr|I4DMS1|I4DMS1_PAPPL 34740.HMEL017774-PA 13.p1 factor 3 subunit M OS=Papilio polytes OX=76194 PE=2 SV=1 tr|A0A5P9L9K9|A0A5P9L9K9_SP TRINITY_DN1467_c0_g1_i tr|A0A5P9L9K9|A0A5P9L9K9_SP OEX HMGB1 OS=Spodoptera 34740.HMEL007340-PA 12.p1 OEX exigua OX=7107 PE=2 SV=1 tr|S4PEK0|S4PEK0_9NEOP Piwi TRINITY_DN2904_c0_g1_i 7091.BGIBMGA010644- tr|S4PEK0|S4PEK0_9NEOP OS=Pararge aegeria OX=116150 11.p1 TA PE=3 SV=1 77

XP_023948152.1 insulin receptor TRINITY_DN1846_c0_g1_i XP_023948152.1 substrate 1-B isoform X2 [Bicyclus 13037.EHJ66806 5.p1 anynana] XP_032513501.1 ecdysone receptor TRINITY_DN4610_c0_g1_i XP_032513501.1 isoform X5 [Danaus plexippus 34740.HMEL012265-PA 12.p1 plexippus] tr|A0A0N1PF91|A0A0N1PF91_PA TRINITY_DN1846_c0_g1_i tr|A0A0N1PF91|A0A0N1PF91_PA PMA Insulin receptor substrate 1-B 13037.EHJ66806 10.p1 PMA OS=Papilio machaon OX=76193 GN=RR48_04182 PE=4 SV=1 UniRef90_UPI001181E1C1 MAP kinase-activating death domain TRINITY_DN6101_c0_g1_i UniRef90_UPI001181E1C1 protein-like n=1 Tax=Manduca 13037.EHJ72087 8.p1 sexta TaxID=7130 RepID=UPI001181E1C1 XP_028179282.1 uncharacterized TRINITY_DN14047_c0_g1 XP_028179282.1 protein LOC114366563, partial 13037.EHJ68996 _i4.p1 [Ostrinia furnacalis] XP_026490648.1 paired TRINITY_DN1156_c0_g1_i XP_026490648.1 amphipathic helix protein Sin3a 13037.EHJ64385 3.p1 isoform X7 [Vanessa tameamea] tr|A0A194Q421|A0A194Q421_PAP XU Phosphatidylinositol 4,5- TRINITY_DN11633_c1_g1 tr|A0A194Q421|A0A194Q421_PAP bisphosphate 3-kinase catalytic 7091.BGIBMGA002921- _i6.p1 XU subunit OS=Papilio xuthus TA OX=66420 GN=RR46_02881 PE=3 SV=1 XP_026489976.1 ubiquitin-like- TRINITY_DN6464_c0_g1_i XP_026489976.1 conjugating enzyme ATG10 34740.HMEL014699-PA 1.p1 [Vanessa tameamea] PCG73649.1 hypothetical protein TRINITY_DN15053_c1_g1 PCG73649.1 B5V51_14577, partial [Heliothis 34740.HMEL014041-PA _i1.p1 virescens] tr|A0A2A4K6C6|A0A2A4K6C6_H ELVI GATA-type domain- TRINITY_DN180_c0_g1_i2 tr|A0A2A4K6C6|A0A2A4K6C6_H containing protein OS=Heliothis 34740.HMEL007452-PA 6.p1 ELVI virescens OX=7102 GN=B5V51_1152 PE=4 SV=1 tr|S4NY13|S4NY13_9NEOP TRINITY_DN11864_c0_g1 tr|S4NY13|S4NY13_9NEOP Zyxin/trip6 OS=Pararge aegeria 34740.HMEL005521-PA _i1.p1 OX=116150 PE=4 SV=1 tr|A0A480KSJ4|A0A480KSJ4_PIG TRINITY_DN13072_c0_g1 Bardet-Biedl syndrome 2 protein tr|A0A480KSJ4|A0A480KSJ4_PIG 13037.EHJ72871 _i13.p2 isoform X1 OS=Sus scrofa OX=9823 PE=4 SV=1 XP_026487085.1 suppressor of TRINITY_DN878_c0_g1_i1 XP_026487085.1 hairless protein isoform X2 13037.EHJ72770 5.p3 [Vanessa tameamea] tr|A0A3Q0CJ71|A0A3Q0CJ71_ME SAU ras-related C3 botulinum toxin TRINITY_DN3012_c2_g1_i tr|A0A3Q0CJ71|A0A3Q0CJ71_ME substrate 3 isoform X2 34740.HMEL007742-PA 2.p1 SAU OS=Mesocricetus auratus OX=10036 GN=Rac3 PE=4 SV=1 tr|A0A0E3IQG1|A0A0E3IQG1_SP TRINITY_DN5795_c0_g2_i tr|A0A0E3IQG1|A0A0E3IQG1_SP OLT Autophagy related protein 1 34740.HMEL017558-PA 2.p1 OLT isoform A OS=Spodoptera litura OX=69820 GN=Atg1 PE=2 SV=1 78

tr|A0A1U9VYP0|A0A1U9VYP0_H ELAM Phosphatidylinositol 3,4,5- TRINITY_DN13355_c0_g1 tr|A0A1U9VYP0|A0A1U9VYP0_H trisphosphate 3-phosphatase and 13037.EHJ66567 _i1.p1 ELAM dual-specificity protein phosphatase OS=Helicoverpa armigera OX=29058 GN=PTEN PE=2 SV=1 tr|A0A3S2LP30|A0A3S2LP30_CHI SP Protein kinase domain- TRINITY_DN5795_c0_g1_i tr|A0A3S2LP30|A0A3S2LP30_CHI containing protein OS=Chilo 34740.HMEL017558-PA 3.p1 SP suppressalis OX=168631 GN=evm_004421 PE=4 SV=1 tr|A0A2A4K523|A0A2A4K523_HE LVI Protein kinase domain- TRINITY_DN176_c3_g1_i9 tr|A0A2A4K523|A0A2A4K523_HE containing protein OS=Heliothis 13037.EHJ67415 .p1 LVI virescens OX=7102 GN=B5V51_2331 PE=4 SV=1 sp|A1A4L5|ALKB8_BOVIN Alkylated DNA repair protein alkB TRINITY_DN17615_c0_g1 sp|A1A4L5|ALKB8_BOVIN homolog 8 OS=Bos taurus 34740.HMEL017714-PA _i19.p2 OX=9913 GN=ALKBH8 PE=2 SV=1 tr|A0A2A4JPH1|A0A2A4JPH1_HE LVI SERPIN domain-containing TRINITY_DN3454_c0_g1_i tr|A0A2A4JPH1|A0A2A4JPH1_HE protein OS=Heliothis virescens 34740.HMEL006260-PA 12.p1 LVI OX=7102 GN=B5V51_14139 PE=3 SV=1 tr|A0A2H1W1A6|A0A2H1W1A6_ SPOFR SFRICE_031245 TRINITY_DN17515_c0_g1 tr|A0A2H1W1A6|A0A2H1W1A6_ OS=Spodoptera frugiperda 34740.HMEL007549-PA _i1.p1 SPOFR OX=7108 GN=SFRICE_031245 PE=4 SV=1 TRINITY_DN17493_c0_g1 13037.EHJ70551 _i1.p2 XP_026486456.1 apoptosis- TRINITY_DN12952_c0_g2 XP_026486456.1 resistant E3 ubiquitin protein ligase 13037.EHJ69238 _i21.p1 1 isoform X1 [Vanessa tameamea] tr|A0A2A4JNA7|A0A2A4JNA7_H TRINITY_DN1743_c0_g1_i tr|A0A2A4JNA7|A0A2A4JNA7_H ELVI Uncharacterized protein 13037.EHJ69387 17.p1 ELVI OS=Heliothis virescens OX=7102 GN=B5V51_14794 PE=4 SV=1 XP_028923669.1 apoptosis- TRINITY_DN2327_c0_g3_i inducing factor 1, mitochondrial XP_028923669.1 13037.EHJ74960 3.p3 isoform X2 [Ornithorhynchus anatinus] XP_026491088.1 vascular TRINITY_DN192_c3_g1_i6 XP_026491088.1 endothelial growth factor receptor 1 13037.EHJ78656 .p1 [Vanessa tameamea] XP_032521661.1 63 kDa TRINITY_DN14883_c0_g1 XP_032521661.1 chaperonin, mitochondrial-like 13037.EHJ77502 _i1.p1 [Danaus plexippus plexippus] tr|S4PG76|S4PG76_9NEOP Singed TRINITY_DN1720_c0_g1_i tr|S4PG76|S4PG76_9NEOP OS=Pararge aegeria OX=116150 13037.EHJ76110 1.p2 PE=4 SV=1 tr|A0A2A4JYP8|A0A2A4JYP8_HE LVI PPM-type phosphatase domain- TRINITY_DN1719_c0_g1_i tr|A0A2A4JYP8|A0A2A4JYP8_HE containing protein OS=Heliothis 13037.EHJ74899 1.p1 LVI virescens OX=7102 GN=B5V51_8656 PE=3 SV=1 79

tr|A0A194PIX4|A0A194PIX4_PAP TRINITY_DN5178_c0_g1_i tr|A0A194PIX4|A0A194PIX4_PAP XU Uncharacterized protein 13037.EHJ69185 10.p1 XU OS=Papilio xuthus OX=66420 GN=RR46_10532 PE=4 SV=1 TRINITY_DN3161_c0_g1_i XP_026490693.1 protein LLP XP_026490693.1 34740.HMEL016080-PA 4.p2 homolog [Vanessa tameamea] UniRef90_A0A5E4QQN2 Uncharacterized protein (Fragment) TRINITY_DN52498_c0_g1 UniRef90_A0A5E4QQN2 n=1 Tax=Leptidea sinapis 34740.HMEL013671-PA _i1.p2 TaxID=189913 RepID=A0A5E4QQN2_9NEOP XP_030039579.1 WAS/WASL- TRINITY_DN1710_c6_g1_i XP_030039579.1 interacting protein family member 13037.EHJ73699 3.p1 1-like, partial [Manduca sexta] XP_026501183.1 raf homolog TRINITY_DN17059_c0_g2 XP_026501183.1 serine/threonine-protein kinase Raf 13037.EHJ72756 _i6.p1 isoform X1 [Vanessa tameamea] XP_026501184.1 raf homolog TRINITY_DN17059_c0_g1 XP_026501184.1 serine/threonine-protein kinase Raf 13037.EHJ72756 _i1.p1 isoform X2 [Vanessa tameamea] TRINITY_DN17046_c0_g5 XP_022124024.1 signal transducing XP_022124024.1 13037.EHJ69304 _i2.p2 adapter molecule 1 [Pieris rapae] tr|A0A212ETQ8|A0A212ETQ8_D ANPL TRAF3-interacting protein 1 TRINITY_DN15034_c0_g1 tr|A0A212ETQ8|A0A212ETQ8_D OS=Danaus plexippus plexippus 13037.EHJ67958 _i2.p1 ANPL OX=278856 GN=KGM_202322 PE=4 SV=1 tr|S4NI94|S4NI94_9NEOP Ring TRINITY_DN16849_c0_g1 tr|S4NI94|S4NI94_9NEOP box protein OS=Pararge aegeria 34740.HMEL017734-PA _i1.p1 OX=116150 PE=4 SV=1 tr|A0A2W1BUZ9|A0A2W1BUZ9_ HELAM Spaetzle domain- TRINITY_DN16774_c0_g1 tr|A0A2W1BUZ9|A0A2W1BUZ9_ containing protein OS=Helicoverpa 13037.EHJ74226 _i2.p1 HELAM armigera OX=29058 GN=HaOG203363 PE=4 SV=1 tr|A0A2A4K9Y7|A0A2A4K9Y7_H ELVI Protein kinase domain- TRINITY_DN1114_c0_g2_i tr|A0A2A4K9Y7|A0A2A4K9Y7_H containing protein OS=Heliothis 13037.EHJ75547 5.p1 ELVI virescens OX=7102 GN=B5V51_37 PE=4 SV=1 tr|S4P8N0|S4P8N0_9NEOP Cell TRINITY_DN16658_c0_g1 tr|S4P8N0|S4P8N0_9NEOP death activator CIDE-B OS=Pararge 34740.HMEL010968-PA _i1.p1 aegeria OX=116150 PE=4 SV=1 tr|A0A0N1PF91|A0A0N1PF91_PA TRINITY_DN1846_c0_g1_i tr|A0A0N1PF91|A0A0N1PF91_PA PMA Insulin receptor substrate 1-B 13037.EHJ66806 8.p1 PMA OS=Papilio machaon OX=76193 GN=RR48_04182 PE=4 SV=1 tr|A0A194QVL5|A0A194QVL5_P TRINITY_DN3865_c1_g1_i tr|A0A194QVL5|A0A194QVL5_P APMA Protein bicaudal D 34740.HMEL013410-PA 3.p1 APMA OS=Papilio machaon OX=76193 GN=RR48_13212 PE=4 SV=1 XP_026492691.1 G1/S-specific TRINITY_DN15638_c0_g1 XP_026492691.1 cyclin-E isoform X2 [Vanessa 34740.HMEL009212-PA _i3.p1 tameamea] 80

tr|A0A2A4JV18|A0A2A4JV18_HE LVI ENTH domain-containing TRINITY_DN1720_c0_g1_i tr|A0A2A4JV18|A0A2A4JV18_HE 7091.BGIBMGA005700- protein OS=Heliothis virescens 1.p1 LVI TA OX=7102 GN=B5V51_12035 PE=4 SV=1 XP_026495181.1 multiple inositol TRINITY_DN18446_c0_g1 XP_026495181.1 polyphosphate phosphatase 1-like 13037.EHJ78228 _i3.p1 [Vanessa tameamea] UniRef90_A0A0K2F2G9 Cinnamyl alcohol dehydrogenase (Fragment) TRINITY_DN161579_c0_g 3694.POPTR_0016s06540 UniRef90_A0A0K2F2G9 n=1 Tax=Silybum marianum 1_i1.p1 .1 TaxID=92921 RepID=A0A0K2F2G9_SILMA XP_026499875.1 GRB2-associated- TRINITY_DN16148_c0_g1 XP_026499875.1 binding protein 1 isoform X1 13037.EHJ69343 _i21.p1 [Vanessa tameamea] tr|S4NXV2|S4NXV2_9NEOP TRINITY_DN14982_c0_g2 7091.BGIBMGA012709- tr|S4NXV2|S4NXV2_9NEOP Akirin OS=Pararge aegeria _i4.p1 TA OX=116150 PE=4 SV=1 tr|A0A3P8QYA7|A0A3P8QYA7_A TRINITY_DN5023_c0_g1_i tr|A0A3P8QYA7|A0A3P8QYA7_A STCA Pre-B-cell leukemia 34740.HMEL015430-PA 4.p2 STCA homeobox 1a OS=Astatotilapia calliptera OX=8154 PE=4 SV=1 TRINITY_DN160138_c0_g 34740.HMEL013671-PA 1_i1.p1 sp|P01512|ATTB_HYACE Attacin- TRINITY_DN26591_c0_g1 sp|P01512|ATTB_HYACE B OS=Hyalophora cecropia 13037.EHJ76138 _i3.p1 OX=7123 PE=2 SV=2 TRINITY_DN18542_c0_g2 XP_022829699.1 peroxiredoxin 1 XP_022829699.1 34740.HMEL015335-PA _i4.p1 isoform X3 [Spodoptera litura] tr|A0A212EWQ2|A0A212EWQ2_ DANPL Non-specific TRINITY_DN3369_c0_g1_i tr|A0A212EWQ2|A0A212EWQ2_ serine/threonine protein kinase 13037.EHJ69136 7.p1 DANPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_203565 PE=4 SV=1 TRINITY_DN15936_c0_g1 13037.EHJ64104 _i1.p1 XP_026500259.1 peptidoglycan TRINITY_DN158_c0_g3_i1 7091.BGIBMGA000586- XP_026500259.1 recognition protein-like isoform X1 .p1 TA [Vanessa tameamea] tr|A0A212F9B2|A0A212F9B2_DA TRINITY_DN14161_c0_g1 tr|A0A212F9B2|A0A212F9B2_DA NPL Galectin OS=Danaus 13037.EHJ73865 _i1.p1 NPL plexippus plexippus OX=278856 GN=KGM_212469 PE=4 SV=1 tr|H9CNL0|H9CNL0_SPOEX TRINITY_DN1584_c0_g2_i tr|H9CNL0|H9CNL0_SPOEX Ryanodien receptor OS=Spodoptera 13037.EHJ73734 1.p1 exigua OX=7107 PE=2 SV=1 XP_026485383.1 probable ATP- TRINITY_DN1045_c0_g1_i XP_026485383.1 dependent RNA helicase pitchoune 34740.HMEL007864-PA 16.p1 [Vanessa tameamea] tr|A0A194Q0H6|A0A194Q0H6_PA TRINITY_DN15805_c0_g2 tr|A0A194Q0H6|A0A194Q0H6_PA PXU ML domain-containing protein 13037.EHJ76124 _i1.p1 PXU OS=Papilio xuthus OX=66420 GN=RR46_10400 PE=4 SV=1 81

tr|A0A2A4KAC5|A0A2A4KAC5_ HELVI Protein kinase domain- TRINITY_DN1577_c0_g1_i tr|A0A2A4KAC5|A0A2A4KAC5_ containing protein OS=Heliothis 34740.HMEL011423-PA 12.p1 HELVI virescens OX=7102 GN=B5V51_1110 PE=4 SV=1 UniRef90_UPI0009956BAF uncharacterized protein TRINITY_DN25968_c0_g1 LOC109862357 n=1 UniRef90_UPI0009956BAF 13037.EHJ64423 _i1.p3 Tax=Pseudomyrmex gracilis TaxID=219809 RepID=UPI0009956BAF tr|S4P9P6|S4P9P6_9NEOP TRINITY_DN1576_c0_g1_i Maternal expression at 31B tr|S4P9P6|S4P9P6_9NEOP 13037.EHJ66501 7.p1 OS=Pararge aegeria OX=116150 PE=3 SV=1 tr|A0A3S2PH19|A0A3S2PH19_CH ISP GCM domain-containing TRINITY_DN54480_c0_g1 tr|A0A3S2PH19|A0A3S2PH19_CH protein OS=Chilo suppressalis 34740.HMEL005671-PA _i2.p1 ISP OX=168631 GN=evm_004195 PE=4 SV=1 tr|A0A5E4R3S5|A0A5E4R3S5_9N EOP Protein kinase domain- TRINITY_DN13160_c0_g1 tr|A0A5E4R3S5|A0A5E4R3S5_9N containing protein OS=Leptidea 34740.HMEL003520-PA _i5.p1 EOP sinapis OX=189913 GN=LSINAPIS_LOCUS14859 PE=3 SV=1 tr|A0A212ERI5|A0A212ERI5_DA NPL Dynein heavy chain TRINITY_DN650_c0_g1_i4 tr|A0A212ERI5|A0A212ERI5_DA OS=Danaus plexippus plexippus 13037.EHJ67053 .p1 NPL OX=278856 GN=KGM_202523 PE=4 SV=1 tr|A0A212F5X3|A0A212F5X3_DA NPL Immune-related Hdd1 TRINITY_DN25330_c0_g1 tr|A0A212F5X3|A0A212F5X3_DA OS=Danaus plexippus plexippus 34740.HMEL007377-PA _i1.p1 NPL OX=278856 GN=KGM_213324 PE=4 SV=1 TRINITY_DN17119_c0_g1 7091.BGIBMGA001303-

_i3.p1 TA TRINITY_DN1978_c4_g1_i XP_023936959.1 toll-like receptor XP_023936959.1 34740.HMEL006338-PA 4.p1 6 [Bicyclus anynana] TRINITY_DN9917_c0_g1_i XP_026495256.1 integrin beta-PS XP_026495256.1 34740.HMEL005744-PA 10.p1 [Vanessa tameamea] tr|A0A212ENN6|A0A212ENN6_D ANPL E2F transcription factor 1 TRINITY_DN4791_c0_g1_i tr|A0A212ENN6|A0A212ENN6_D 7091.BGIBMGA003403- OS=Danaus plexippus plexippus 6.p1 ANPL TA OX=278856 GN=KGM_208741 PE=3 SV=1 TRINITY_DN3461_c0_g1_i XP_026484381.1 protein sickie XP_026484381.1 13037.EHJ78388 5.p1 isoform X6 [Vanessa tameamea] UniRef90_A0A4C1V6H7 Casein kinase I isoform gamma-3 n=1 TRINITY_DN1124_c4_g2_i UniRef90_A0A4C1V6H7 Tax=Eumeta variegata 13037.EHJ70836 4.p1 TaxID=151549 RepID=A0A4C1V6H7_EUMVA XP_026494146.1 phenoloxidase- TRINITY_DN15341_c0_g1 XP_026494146.1 activating factor 1-like [Vanessa 34740.HMEL014233-PA _i2.p1 tameamea] 82

tr|A0A2A4JJE5|A0A2A4JJE5_HEL VI Cytochrome b5 heme-binding TRINITY_DN15304_c0_g1 tr|A0A2A4JJE5|A0A2A4JJE5_HEL domain-containing protein 34740.HMEL005896-PA _i1.p2 VI OS=Heliothis virescens OX=7102 GN=B5V51_1170 PE=3 SV=1 tr|A0A194QAX3|A0A194QAX3_P TRINITY_DN36035_c0_g1 tr|A0A194QAX3|A0A194QAX3_P APXU Hemicentin-1 OS=Papilio 34740.HMEL007228-PA _i1.p1 APXU xuthus OX=66420 GN=RR46_07161 PE=4 SV=1 XP_028037332.1 activating TRINITY_DN1295_c4_g1_i molecule in BECN1-regulated XP_028037332.1 13037.EHJ65700 22.p1 autophagy protein 1-like, partial [Bombyx mandarina] XP_026494152.1 phenoloxidase- TRINITY_DN1682_c0_g1_i XP_026494152.1 activating factor 2-like [Vanessa 34740.HMEL013468-PA 4.p1 tameamea] TRINITY_DN22181_c0_g1 34740.HMEL011289-PA _i2.p3 TRINITY_DN6613_c1_g1_i 34740.HMEL003132-PA 15.p2 TRINITY_DN4402_c0_g1_i XP_026497302.1 serpin B3 XP_026497302.1 34740.HMEL007479-PA 1.p1 [Vanessa tameamea] tr|H9CNL0|H9CNL0_SPOEX TRINITY_DN1584_c0_g1_i tr|H9CNL0|H9CNL0_SPOEX Ryanodien receptor OS=Spodoptera 13037.EHJ73734 2.p1 exigua OX=7107 PE=2 SV=1 TRINITY_DN1743_c0_g1_i XP_026485436.1 protein lap4-like XP_026485436.1 13037.EHJ69387 10.p1 isoform X9 [Vanessa tameamea] tr|A0A517BDX4|A0A517BDX4_H ELME 1-phosphatidylinositol 4,5- TRINITY_DN3797_c0_g1_i tr|A0A517BDX4|A0A517BDX4_H bisphosphate phosphodiesterase 34740.HMEL013005-PA 1.p1 ELME OS=Heliconius melpomene OX=34740 PE=2 SV=1 tr|W6D4F0|W6D4F0_PANTA TRINITY_DN145774_c0_g Vimentin (Fragment) OS=Panthera 61853.ENSNLEP0000001 tr|W6D4F0|W6D4F0_PANTA 1_i1.p1 tigris altaica OX=74533 GN=VIM 5266 PE=2 SV=1 tr|A0A212ET32|A0A212ET32_DA TRINITY_DN20565_c0_g1 tr|A0A212ET32|A0A212ET32_DA NPL Serpin-27 OS=Danaus 34740.HMEL006662-PA _i2.p1 NPL plexippus plexippus OX=278856 GN=KGM_202595 PE=3 SV=1 tr|S4PL69|S4PL69_9NEOP U4/U6.U5 small nuclear TRINITY_DN1498_c0_g1_i tr|S4PL69|S4PL69_9NEOP ribonucleoprotein 27 kDa protein 13037.EHJ69936 10.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 tr|A0A212F5T0|A0A212F5T0_DA NPL Uncharacterized protein TRINITY_DN19682_c0_g1 tr|A0A212F5T0|A0A212F5T0_DA OS=Danaus plexippus plexippus 13037.EHJ72578 _i2.p1 NPL OX=278856 GN=KGM_203439 PE=4 SV=1 tr|A0A0L7KRS6|A0A0L7KRS6_9 NEOP BmGATA beta isoform 2- TRINITY_DN180_c0_g1_i1 tr|A0A0L7KRS6|A0A0L7KRS6_9 7091.BGIBMGA002156- silkworm OS=Operophtera brumata 9.p2 NEOP TA OX=104452 GN=OBRU01_22265 PE=4 SV=1 83

tr|A0A212FL92|A0A212FL92_DA TRINITY_DN6665_c0_g1_i tr|A0A212FL92|A0A212FL92_DA NPL Decapentaplegic OS=Danaus 13037.EHJ78539 7.p1 NPL plexippus plexippus OX=278856 GN=KGM_214072 PE=3 SV=1 tr|A0A2H1VUD8|A0A2H1VUD8_ SPOFR SFRICE_020181 TRINITY_DN8735_c0_g2_i tr|A0A2H1VUD8|A0A2H1VUD8_ OS=Spodoptera frugiperda 13037.EHJ65560 5.p2 SPOFR OX=7108 GN=SFRICE_020181 PE=4 SV=1 tr|A0A2A4K4M5|A0A2A4K4M5_ TRINITY_DN147198_c0_g tr|A0A2A4K4M5|A0A2A4K4M5_ HELVI Tetraspanin OS=Heliothis 34740.HMEL017773-PA 1_i1.p1 HELVI virescens OX=7102 GN=B5V51_3651 PE=3 SV=1 UniRef90_A0A1E1WLP9 Uncharacterized protein (Fragment) TRINITY_DN2039_c0_g1_i UniRef90_A0A1E1WLP9 n=2 Tax=Pectinophora gossypiella 13037.EHJ65548 5.p2 TaxID=13191 RepID=A0A1E1WLP9_PECGO TRINITY_DN14702_c0_g1 XP_026486587.1 protein eiger XP_026486587.1 13037.EHJ68630 _i2.p1 [Vanessa tameamea] XP_025407421.1 zinc finger TRINITY_DN23792_c0_g1 XP_025407421.1 MYM-type protein 1-like [Sipha 7029.ACYPI27979-PA _i19.p4 flava] XP_026495341.1 probable nuclear TRINITY_DN1686_c0_g1_i 7091.BGIBMGA006966- XP_026495341.1 transport factor 2 isoform X1 11.p1 TA [Vanessa tameamea] UniRef90_A0A0J9R141 Uncharacterized protein, isoform B TRINITY_DN14646_c0_g1 UniRef90_A0A0J9R141 n=1 Tax=Drosophila simulans 13037.EHJ73069 _i5.p2 TaxID=7240 RepID=A0A0J9R141_DROSI TRINITY_DN146298_c0_g EDW26942.1 GL21429 [Drosophila EDW26942.1 13037.EHJ65580 1_i1.p1 persimilis] XP_026501735.1 probable ATP- TRINITY_DN34493_c0_g1 XP_026501735.1 dependent RNA helicase DHX35 34740.HMEL022585-PA _i4.p1 [Vanessa tameamea] XP_028172844.1 disintegrin and TRINITY_DN1460_c0_g1_i metalloproteinase domain- XP_028172844.1 34740.HMEL014673-PA 2.p1 containing protein 10 isoform X1 [Ostrinia furnacalis] XP_026492166.1 serine protease TRINITY_DN1459_c0_g1_i XP_026492166.1 persephone-like [Vanessa 34740.HMEL011930-PA 1.p1 tameamea] TRINITY_DN1928_c0_g1_i XP_032517981.1 antitrypsin-like XP_032517981.1 34740.HMEL006260-PA 12.p1 [Danaus plexippus plexippus] tr|A0A212FNV6|A0A212FNV6_D TRINITY_DN1947_c0_g1_i tr|A0A212FNV6|A0A212FNV6_D ANPL Caspase Nc OS=Danaus 13037.EHJ74014 10.p1 ANPL plexippus plexippus OX=278856 GN=KGM_205925 PE=3 SV=1 tr|S4NP35|S4NP35_9NEOP TRINITY_DN2418_c0_g2_i Arsenite-resistance protein tr|S4NP35|S4NP35_9NEOP 34740.HMEL013320-PA 2.p1 (Fragment) OS=Pararge aegeria OX=116150 PE=4 SV=1 tr|A0A0N0PCT2|A0A0N0PCT2_P APMA CAP-Gly domain- TRINITY_DN114788_c0_g tr|A0A0N0PCT2|A0A0N0PCT2_P containing protein OS=Papilio 34740.HMEL008248-PA 1_i1.p1 APMA machaon OX=76193 GN=RR48_01400 PE=4 SV=1 84

UniRef90_A9QTK1 Catalase (Fragment) n=1 TRINITY_DN144532_c0_g UniRef90_A9QTK1 Tax=Staphylococcus gallinarum 13037.EHJ67717 1_i1.p1 TaxID=1293 RepID=A9QTK1_STAGA tr|A0A194QRD1|A0A194QRD1_P TRINITY_DN15603_c0_g1 tr|A0A194QRD1|A0A194QRD1_P APMA Nitric oxide synthase 7091.BGIBMGA002937- _i1.p1 APMA OS=Papilio machaon OX=76193 TA GN=RR48_12741 PE=3 SV=1 tr|A0A212EGT4|A0A212EGT4_D ANPL Seminal fluid protein TRINITY_DN6470_c0_g2_i tr|A0A212EGT4|A0A212EGT4_D CSSFP041 OS=Danaus plexippus 13037.EHJ63159 1.p1 ANPL plexippus OX=278856 GN=KGM_207577 PE=3 SV=1 tr|A0A2A4K064|A0A2A4K064_HE LVI Ubiquitin carboxyl-terminal TRINITY_DN14341_c0_g1 tr|A0A2A4K064|A0A2A4K064_HE hydrolase OS=Heliothis virescens 13037.EHJ72162 _i2.p1 LVI OX=7102 GN=B5V51_7546 PE=3 SV=1 tr|A0A1E1W1N3|A0A1E1W1N3_P ECGO CLIP domain-containing TRINITY_DN9026_c0_g1_i tr|A0A1E1W1N3|A0A1E1W1N3_P serine protease (Fragment) 13037.EHJ64711 1.p2 ECGO OS=Pectinophora gossypiella OX=13191 GN=g.16409 PE=3 SV=1 tr|A0A2W1BKA4|A0A2W1BKA4_ HELAM Eyes absent homolog TRINITY_DN5452_c0_g1_i tr|A0A2W1BKA4|A0A2W1BKA4_ OS=Helicoverpa armigera 13037.EHJ75518 19.p1 HELAM OX=29058 GN=HaOG208254 PE=3 SV=1 tr|A0A212EP52|A0A212EP52_DA NPL F-box/WD repeat-containing TRINITY_DN1429_c0_g2_i tr|A0A212EP52|A0A212EP52_DA protein TBL1X OS=Danaus 34740.HMEL015146-PA 4.p1 NPL plexippus plexippus OX=278856 GN=KGM_204225 PE=4 SV=1 XP_026494174.1 autophagy-related TRINITY_DN287_c0_g1_i1 XP_026494174.1 protein 2 homolog A [Vanessa 34740.HMEL002592-PA .p1 tameamea] tr|A0A212EP52|A0A212EP52_DA NPL F-box/WD repeat-containing TRINITY_DN1429_c0_g1_i tr|A0A212EP52|A0A212EP52_DA protein TBL1X OS=Danaus 34740.HMEL015146-PA 10.p1 NPL plexippus plexippus OX=278856 GN=KGM_204225 PE=4 SV=1 tr|A0A194RBP8|A0A194RBP8_PA PMA Non-specific serine/threonine TRINITY_DN14280_c0_g2 tr|A0A194RBP8|A0A194RBP8_PA protein kinase OS=Papilio machaon 34740.HMEL005748-PA _i36.p1 PMA OX=76193 GN=RR48_09059 PE=3 SV=1 XP_023938372.1 LOW QUALITY TRINITY_DN21492_c0_g1 PROTEIN: inhibitor of nuclear XP_023938372.1 13037.EHJ64423 _i1.p1 factor kappa-B kinase subunit epsilon [Bicyclus anynana] tr|A0A212EXF1|A0A212EXF1_DA TRINITY_DN3413_c0_g1_i tr|A0A212EXF1|A0A212EXF1_DA NPL Lap4 like protein OS=Danaus 13037.EHJ69387 14.p1 NPL plexippus plexippus OX=278856 GN=KGM_211581 PE=4 SV=1 XP_026485198.1 transcription TRINITY_DN180_c0_g1_i1 XP_026485198.1 factor BCFI-like isoform X2 34740.HMEL007452-PA 6.p1 [Vanessa tameamea] 85

TRINITY_DN2039_c0_g1_i PCG63776.1 hypothetical protein PCG63776.1 13037.EHJ65548 14.p3 B5V51_11733 [Heliothis virescens] XP_031768934.1 insulin-like TRINITY_DN15258_c0_g2 XP_031768934.1 receptor isoform X3 [Galleria 34740.HMEL014094-PA _i15.p1 mellonella] tr|A0A212EK97|A0A212EK97_DA NPL SUMO-activating enzyme TRINITY_DN1938_c0_g1_i tr|A0A212EK97|A0A212EK97_DA subunit OS=Danaus plexippus 13037.EHJ64540 1.p1 NPL plexippus OX=278856 GN=KGM_214809 PE=3 SV=1 XP_013137504.1 PREDICTED: TRINITY_DN14022_c0_g1 translin-associated factor X- XP_013137504.1 34740.HMEL010433-PA _i1.p1 interacting protein 1-like isoform X1 [Papilio polytes] tr|A0A437BXX1|A0A437BXX1_C HISP CULLIN_2 domain- TRINITY_DN10079_c0_g1 tr|A0A437BXX1|A0A437BXX1_C containing protein OS=Chilo 13037.EHJ73775 _i3.p1 HISP suppressalis OX=168631 GN=evm_000205 PE=3 SV=1 XP_026498695.1 enhancer of TRINITY_DN6485_c0_g2_i XP_026498695.1 mRNA-decapping protein 4-like, 34740.HMEL012843-PA 4.p1 partial [Vanessa tameamea] XP_026500284.1 UDP-N- acetylglucosamine--peptide N- TRINITY_DN13973_c0_g1 XP_026500284.1 acetylglucosaminyltransferase 110 13037.EHJ65577 _i13.p1 kDa subunit-like [Vanessa tameamea] tr|A0A194PYH1|A0A194PYH1_PA TRINITY_DN24_c0_g3_i4. tr|A0A194PYH1|A0A194PYH1_PA PXU Titin OS=Papilio xuthus 13037.EHJ65548 p1 PXU OX=66420 GN=RR46_06934 PE=4 SV=1 tr|A0A194PT25|A0A194PT25_PAP XU Alpha-1,3-mannosyl- TRINITY_DN1393_c0_g1_i tr|A0A194PT25|A0A194PT25_PAP glycoprotein 2-beta-N- 34740.HMEL011176-PA 1.p1 XU acetylglucosaminyltransferase OS=Papilio xuthus OX=66420 GN=RR46_12497 PE=4 SV=1 XP_026488058.1 PAX-interacting TRINITY_DN2696_c0_g1_i XP_026488058.1 protein 1 isoform X2 [Vanessa 13037.EHJ79047 2.p1 tameamea] tr|A0A2H1VKP2|A0A2H1VKP2_S POFR SFRICE_020889 TRINITY_DN138_c0_g1_i4 tr|A0A2H1VKP2|A0A2H1VKP2_S OS=Spodoptera frugiperda 13037.EHJ70705 .p1 POFR OX=7108 GN=SFRICE_020889 PE=3 SV=1 XP_026497950.1 gamma- TRINITY_DN1100_c0_g1_i XP_026497950.1 glutamylcyclotransferase-like 13037.EHJ65072 11.p1 [Vanessa tameamea] XP_026485909.1 dual specificity TRINITY_DN10613_c0_g1 XP_026485909.1 protein phosphatase 14 [Vanessa 34740.HMEL007565-PA _i2.p1 tameamea] tr|A0A1A8DQZ9|A0A1A8DQZ9_9 TELE CDC42 binding protein TRINITY_DN15342_c0_g1 tr|A0A1A8DQZ9|A0A1A8DQZ9_9 kinase alpha (DMPK-like) 34740.HMEL015661-PB _i10.p1 TELE (Fragment) OS=Nothobranchius kadleci OX=1051664 GN=CDC42BPA PE=4 SV=1 86

XP_026494143.1 beta-1,3-glucan- TRINITY_DN5210_c0_g1_i XP_026494143.1 binding protein 2-like [Vanessa 13037.EHJ67506 1.p1 tameamea] tr|A0A212F9M5|A0A212F9M5_D ANPL Protein kinase domain- TRINITY_DN13832_c0_g1 tr|A0A212F9M5|A0A212F9M5_D containing protein OS=Danaus 13037.EHJ74042 _i1.p1 ANPL plexippus plexippus OX=278856 GN=KGM_203927 PE=4 SV=1 tr|S4PLN0|S4PLN0_9NEOP NTF2- TRINITY_DN13829_c0_g1 7091.BGIBMGA004933- tr|S4PLN0|S4PLN0_9NEOP like export factor 1 OS=Pararge _i1.p2 TA aegeria OX=116150 PE=4 SV=1 XP_026492017.1 phenoloxidase- TRINITY_DN1887_c0_g2_i XP_026492017.1 activating enzyme-like isoform X2 34740.HMEL004369-PA 1.p1 [Vanessa tameamea] tr|A9NJI6|A9NJI6_PIERA Ferritin TRINITY_DN246_c1_g3_i9 tr|A9NJI6|A9NJI6_PIERA OS=Pieris rapae OX=64459 PE=2 34740.HMEL013441-PA .p1 SV=1 XP_026494156.1 phenoloxidase- TRINITY_DN3730_c0_g1_i XP_026494156.1 activating factor 2-like isoform X2 13037.EHJ70457 7.p1 [Vanessa tameamea] tr|A0A2H1VAC5|A0A2H1VAC5_S POFR SFRICE_004479 TRINITY_DN1928_c0_g1_i tr|A0A2H1VAC5|A0A2H1VAC5_S OS=Spodoptera frugiperda 34740.HMEL006260-PA 24.p1 POFR OX=7108 GN=SFRICE_004479 PE=3 SV=1 tr|A0A212F5Z3|A0A212F5Z3_DA NPL FK506-binding protein TRINITY_DN7760_c0_g1_i tr|A0A212F5Z3|A0A212F5Z3_DA OS=Danaus plexippus plexippus 13037.EHJ72625 4.p1 NPL OX=278856 GN=KGM_208016 PE=3 SV=1 XP_031764433.1 LIM domain TRINITY_DN26611_c0_g1 XP_031764433.1 kinase 1-like isoform X1 [Galleria 34740.HMEL009606-PA _i10.p1 mellonella] tr|A0A2H1WFH0|A0A2H1WFH0_ SPOFR Serine/threonine-protein TRINITY_DN17297_c0_g2 tr|A0A2H1WFH0|A0A2H1WFH0_ phosphatase OS=Spodoptera 13037.EHJ70202 _i1.p1 SPOFR frugiperda OX=7108 GN=SFRICE_002550 PE=3 SV=1 tr|A0A3S2M0Z6|A0A3S2M0Z6_C HISP Protein kinase domain- TRINITY_DN7695_c0_g1_i tr|A0A3S2M0Z6|A0A3S2M0Z6_C containing protein OS=Chilo 34740.HMEL013682-PA 15.p1 HISP suppressalis OX=168631 GN=evm_006688 PE=4 SV=1 XP_026493951.1 glycogen synthase TRINITY_DN1690_c1_g2_i XP_026493951.1 kinase-3 beta-like isoform X9 34740.HMEL012308-PA 2.p1 [Vanessa tameamea] XP_026493593.1 paired box protein TRINITY_DN13667_c0_g1 XP_026493593.1 Pax-5 isoform X1 [Vanessa 34740.HMEL003749-PA _i1.p1 tameamea] tr|F6K5S4|F6K5S4_EUPAU TRINITY_DN101554_c0_g Caspase-3 OS=Euphydryas aurinia 7091.BGIBMGA006131- tr|F6K5S4|F6K5S4_EUPAU 1_i1.p1 OX=104503 GN=Casp-3 PE=2 TA SV=1 XP_023938372.1 LOW QUALITY TRINITY_DN21492_c0_g1 PROTEIN: inhibitor of nuclear XP_023938372.1 13037.EHJ64423 _i8.p1 factor kappa-B kinase subunit epsilon [Bicyclus anynana] 87

XP_013194141.1 PREDICTED: TRINITY_DN1356_c0_g1_i uncharacterized protein XP_013194141.1 13037.EHJ66805 1.p3 LOC106137781, partial [Amyelois transitella] tr|A0A0N0PAU6|A0A0N0PAU6_P TRINITY_DN1356_c0_g1_i tr|A0A0N0PAU6|A0A0N0PAU6_P APMA Disks large-like 5 34740.HMEL012721-PA 1.p1 APMA OS=Papilio machaon OX=76193 GN=RR48_04183 PE=4 SV=1 XP_028030540.1 zinc finger TRINITY_DN18838_c0_g1 7091.BGIBMGA004640- XP_028030540.1 protein 32-like [Bombyx _i1.p1 TA mandarina] tr|A0A2A4JG02|A0A2A4JG02_HE LVI IPT/TIG domain-containing TRINITY_DN146531_c0_g tr|A0A2A4JG02|A0A2A4JG02_HE protein OS=Heliothis virescens 13037.EHJ63297 1_i1.p1 LVI OX=7102 GN=B5V51_2357 PE=3 SV=1 XP_026488798.1 RNA TRINITY_DN14646_c0_g1 pseudouridylate synthase domain- XP_026488798.1 34740.HMEL003310-PA _i14.p1 containing protein 2-like isoform X1 [Vanessa tameamea] tr|A0A212FJX3|A0A212FJX3_DA NPL Uncharacterized protein TRINITY_DN6845_c0_g1_i tr|A0A212FJX3|A0A212FJX3_DA OS=Danaus plexippus plexippus 13037.EHJ78030 1.p1 NPL OX=278856 GN=KGM_202467 PE=4 SV=1 tr|S4P807|S4P807_9NEOP TRINITY_DN249_c0_g2_i1 Transcription elongation factor B tr|S4P807|S4P807_9NEOP 13037.EHJ74303 .p1 polypeptide 1 OS=Pararge aegeria OX=116150 PE=3 SV=1 tr|A0A2H1VHU6|A0A2H1VHU6_ SPOFR SFRICE_001471 TRINITY_DN11994_c0_g1 tr|A0A2H1VHU6|A0A2H1VHU6_ OS=Spodoptera frugiperda 34740.HMEL006805-PA _i2.p1 SPOFR OX=7108 GN=SFRICE_001471 PE=3 SV=1 tr|A0A212EKG5|A0A212EKG5_D ANPL Ociad protein isoform 2 TRINITY_DN10255_c0_g1 tr|A0A212EKG5|A0A212EKG5_D OS=Danaus plexippus plexippus 13037.EHJ64591 _i6.p1 ANPL OX=278856 GN=KGM_202995 PE=4 SV=1 TRINITY_DN13440_c0_g1 13037.EHJ66567 _i1.p1 tr|A0A2H1VGB1|A0A2H1VGB1_S POFR SFRICE_004635 TRINITY_DN1298_c0_g1_i tr|A0A2H1VGB1|A0A2H1VGB1_S OS=Spodoptera frugiperda 13037.EHJ76670 13.p1 POFR OX=7108 GN=SFRICE_004635 PE=4 SV=1 tr|A0A212FMW2|A0A212FMW2_ TRINITY_DN2241_c0_g1_i tr|A0A212FMW2|A0A212FMW2_ DANPL Merlin OS=Danaus 13037.EHJ79218 13.p1 DANPL plexippus plexippus OX=278856 GN=KGM_207092 PE=4 SV=1 TRINITY_DN126389_c0_g 34740.HMEL005137-PA 1_i1.p1 XP_023948073.1 disintegrin and TRINITY_DN1460_c0_g1_i metalloproteinase domain- XP_023948073.1 34740.HMEL014673-PA 1.p1 containing protein 10-like [Bicyclus anynana] tr|S4PLX2|S4PLX2_9NEOP Protein TRINITY_DN64761_c0_g1 tr|S4PLX2|S4PLX2_9NEOP TIS11 OS=Pararge aegeria 13037.EHJ75049 _i1.p1 OX=116150 PE=4 SV=1 88

tr|C9DTM4|C9DTM4_BOMMO TRINITY_DN1298_c0_g2_i 7091.BGIBMGA012467- tr|C9DTM4|C9DTM4_BOMMO CAP isoform A OS=Bombyx mori 6.p1 TA OX=7091 PE=2 SV=1 tr|S4P2F4|S4P2F4_9NEOP Ragulator complex protein TRINITY_DN14068_c0_g1 tr|S4P2F4|S4P2F4_9NEOP LAMTOR3-like protein 13037.EHJ74735 _i1.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 XP_032527476.1 anaphase- TRINITY_DN13454_c0_g1 XP_032527476.1 promoting complex subunit 15-like 13037.EHJ66679 _i1.p1 [Danaus plexippus plexippus] tr|A0A212ESQ2|A0A212ESQ2_DA NPL Phosphoinositide 3-kinase TRINITY_DN1936_c1_g1_i tr|A0A212ESQ2|A0A212ESQ2_DA regulatory subunit 4 OS=Danaus 13037.EHJ67557 1.p1 NPL plexippus plexippus OX=278856 GN=KGM_202575 PE=4 SV=1 tr|F6ME18|F6ME18_BOMMO Ken TRINITY_DN12614_c0_g1 tr|F6ME18|F6ME18_BOMMO and barbie protein OS=Bombyx 34740.HMEL009095-PA _i1.p1 mori OX=7091 PE=2 SV=1 XP_026490075.1 uncharacterized TRINITY_DN11311_c0_g1 XP_026490075.1 protein LOC113396373 [Vanessa 13037.EHJ68804 _i1.p1 tameamea] TRINITY_DN6141_c0_g3_i XP_026490649.1 toll-like receptor XP_026490649.1 34740.HMEL016131-PA 1.p1 7 [Vanessa tameamea] KTF82947.1 hypothetical protein TRINITY_DN2086_c0_g1_i KTF82947.1 cypCar_00035906, partial 13037.EHJ67216 1.p8 [Cyprinus carpio] tr|A0A194QN18|A0A194QN18_PA TRINITY_DN1295_c2_g4_i tr|A0A194QN18|A0A194QN18_PA PMA Lysozyme OS=Papilio 13037.EHJ67777 1.p1 PMA machaon OX=76193 GN=RR48_11406 PE=3 SV=1 UniRef90_A0A0T6B6Y0 Immunoglobulin (Fragment) n=1 TRINITY_DN3115_c1_g2_i UniRef90_A0A0T6B6Y0 Tax=Oryctes borbonicus 13037.EHJ65548 11.p2 TaxID=1629725 RepID=A0A0T6B6Y0_9SCAR TRINITY_DN11280_c0_g1 XP_032530256.1 protein Wnt-1 XP_032530256.1 34740.HMEL022601-PA _i20.p1 [Danaus plexippus plexippus] tr|A0A212FLJ2|A0A212FLJ2_DA NPL MARVEL domain-containing TRINITY_DN17769_c0_g1 tr|A0A212FLJ2|A0A212FLJ2_DA protein (Fragment) OS=Danaus 13037.EHJ78735 _i1.p1 NPL plexippus plexippus OX=278856 GN=KGM_206087B PE=4 SV=1 UniRef90_A0A4C1V6H7 Casein kinase I isoform gamma-3 n=1 TRINITY_DN1124_c4_g2_i UniRef90_A0A4C1V6H7 Tax=Eumeta variegata 13037.EHJ70836 14.p1 TaxID=151549 RepID=A0A4C1V6H7_EUMVA TRINITY_DN9631_c0_g2_i 34740.HMEL007702-PA 2.p1 XP_026489627.1 CLIP domain- TRINITY_DN5016_c0_g1_i XP_026489627.1 containing serine protease 2 isoform 13037.EHJ78263 1.p1 X1 [Vanessa tameamea] XP_026495409.1 tumor TRINITY_DN3235_c0_g2_i XP_026495409.1 susceptibility gene 101 protein 34740.HMEL006358-PA 1.p2 [Vanessa tameamea] 89

tr|A0A212EQ34|A0A212EQ34_DA NPL Ecdysone receptor B1 isoform TRINITY_DN4610_c0_g1_i tr|A0A212EQ34|A0A212EQ34_DA OS=Danaus plexippus plexippus 13037.EHJ66465 3.p1 NPL OX=278856 GN=KGM_208114 PE=3 SV=1 tr|S4PMW5|S4PMW5_9NEOP Dolichyl-diphosphooligosaccharide- TRINITY_DN22779_c0_g1 tr|S4PMW5|S4PMW5_9NEOP -protein glycosyltransferase subunit 34740.HMEL009204-PA _i10.p1 2 OS=Pararge aegeria OX=116150 PE=3 SV=1 TRINITY_DN11184_c0_g1 13037.EHJ71623 _i15.p1 tr|A0A212F436|A0A212F436_DA TRINITY_DN3708_c0_g1_i tr|A0A212F436|A0A212F436_DA NPL Catalase OS=Danaus 13037.EHJ71937 3.p1 NPL plexippus plexippus OX=278856 GN=KGM_209670 PE=3 SV=1 UniRef90_A0A0L7LRA9 Uncharacterized protein n=1 TRINITY_DN103614_c0_g UniRef90_A0A0L7LRA9 Tax=Operophtera brumata 34740.HMEL013671-PA 1_i1.p1 TaxID=104452 RepID=A0A0L7LRA9_9NEOP tr|H9IUP2|H9IUP2_BOMMO SH3 TRINITY_DN7771_c0_g1_i domain-containing protein tr|H9IUP2|H9IUP2_BOMMO 13037.EHJ66028 3.p2 OS=Bombyx mori OX=7091 PE=4 SV=1 XP_026501394.1 apoptosis- TRINITY_DN425_c0_g1_i1 XP_026501394.1 inducing factor 3 isoform X1 34740.HMEL008334-PA 0.p1 [Vanessa tameamea] tr|S4PXZ6|S4PXZ6_9NEOP SRC TRINITY_DN23979_c0_g1 kinase signaling inhibitor 1 tr|S4PXZ6|S4PXZ6_9NEOP 13037.EHJ75663 _i1.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 XP_023942041.1 adenosine TRINITY_DN6338_c0_g1_i XP_023942041.1 deaminase 2-A-like [Bicyclus 34740.HMEL004268-PA 1.p1 anynana] XP_026496056.1 suppressor of TRINITY_DN1103_c1_g1_i XP_026496056.1 cytokine signaling 5 isoform X3 13037.EHJ74877 1.p1 [Vanessa tameamea] tr|I4DNI1|I4DNI1_PAPXU TRINITY_DN1100_c0_g2_i Gamma-glutamylcyclotransferase 7091.BGIBMGA003537- tr|I4DNI1|I4DNI1_PAPXU 5.p1 OS=Papilio xuthus OX=66420 TA GN=RR46_12008 PE=2 SV=1 tr|A0A212F652|A0A212F652_DA TRINITY_DN10704_c0_g1 tr|A0A212F652|A0A212F652_DA NPL Cysteine protease OS=Danaus 13037.EHJ72670 _i1.p1 NPL plexippus plexippus OX=278856 GN=KGM_214126 PE=3 SV=1 UniRef90_UPI00102E20F4 LOW QUALITY PROTEIN: neurotrophin TRINITY_DN130433_c0_g UniRef90_UPI00102E20F4 1-like n=1 Tax=Monomorium 13037.EHJ66092 1_i1.p1 pharaonis TaxID=307658 RepID=UPI00102E20F4 XP_026492014.1 phenoloxidase- TRINITY_DN246_c0_g2_i1 XP_026492014.1 activating enzyme-like [Vanessa 13037.EHJ73646 .p1 tameamea] tr|A0A437AXM1|A0A437AXM1_ CHISP SERPIN domain-containing TRINITY_DN8950_c0_g1_i tr|A0A437AXM1|A0A437AXM1_ protein OS=Chilo suppressalis 13037.EHJ65951 1.p1 CHISP OX=168631 GN=evm_012537 PE=3 SV=1 90

tr|A0A3S2LP30|A0A3S2LP30_CHI SP Protein kinase domain- TRINITY_DN5795_c0_g1_i tr|A0A3S2LP30|A0A3S2LP30_CHI containing protein OS=Chilo 34740.HMEL017558-PA 6.p1 SP suppressalis OX=168631 GN=evm_004421 PE=4 SV=1 tr|S4NXV2|S4NXV2_9NEOP TRINITY_DN14982_c0_g1 tr|S4NXV2|S4NXV2_9NEOP Akirin OS=Pararge aegeria 13037.EHJ68812 _i1.p1 OX=116150 PE=4 SV=1 TRINITY_DN3431_c1_g1_i XP_026485861.1 afadin isoform X3 XP_026485861.1 34740.HMEL018030-PA 15.p1 [Vanessa tameamea] tr|A0A212F0F5|A0A212F0F5_DA NPL Inorganic pyrophosphatase TRINITY_DN13993_c1_g1 tr|A0A212F0F5|A0A212F0F5_DA OS=Danaus plexippus plexippus 13037.EHJ70548 _i1.p1 NPL OX=278856 GN=KGM_211475 PE=4 SV=1 TRINITY_DN157425_c0_g 13037.EHJ66092 1_i1.p1 XP_026484945.1 ATP-dependent TRINITY_DN6843_c0_g1_i XP_026484945.1 RNA helicase DDX3X isoform X2 34740.HMEL004307-PA 2.p1 [Vanessa tameamea] TRINITY_DN4118_c1_g1_i XP_023228916.1 integrin alpha- XP_023228916.1 13037.EHJ68673 14.p1 PS2-like [Centruroides sculpturatus] tr|A0A212FGD1|A0A212FGD1_D ANPL Ankyrin repeat and SOCS TRINITY_DN1661_c0_g1_i tr|A0A212FGD1|A0A212FGD1_D box protein 3 OS=Danaus plexippus 34740.HMEL008842-PA 10.p1 ANPL plexippus OX=278856 GN=KGM_206607 PE=4 SV=1 XP_026484944.1 ATP-dependent TRINITY_DN6843_c0_g1_i XP_026484944.1 RNA helicase DDX3X isoform X1 34740.HMEL004307-PA 4.p1 [Vanessa tameamea] tr|A0A212FFE6|A0A212FFE6_DA TRINITY_DN5982_c0_g1_i tr|A0A212FFE6|A0A212FFE6_DA NPL GATAd OS=Danaus plexippus 34740.HMEL010830-PA 19.p1 NPL plexippus OX=278856 GN=KGM_214691 PE=4 SV=1 TRINITY_DN1395_c0_g2_i XP_026497725.1 lysozyme-like XP_026497725.1 34740.HMEL002874-PA 3.p1 isoform X1 [Vanessa tameamea] tr|A0A212EY13|A0A212EY13_DA TRINITY_DN114272_c0_g tr|A0A212EY13|A0A212EY13_DA NPL Atonal OS=Danaus plexippus 13037.EHJ69641 1_i1.p1 NPL plexippus OX=278856 GN=KGM_204446 PE=4 SV=1 UniRef90_UPI0005D09892 serine/threonine-protein kinase TRINITY_DN18_c2_g3_i3. UniRef90_UPI0005D09892 MARK2-like n=1 Tax=Plutella 34740.HMEL011289-PA p1 xylostella TaxID=51655 RepID=UPI0005D09892 TRINITY_DN1846_c0_g1_i KPI99751.1 Insulin receptor KPI99751.1 34740.HMEL012720-PA 9.p1 substrate 1 [Papilio xuthus] TRINITY_DN26611_c0_g1 GBP71901.1 LIM domain kinase 1 GBP71901.1 34740.HMEL009606-PA _i5.p2 [Eumeta japonica] TRINITY_DN3118_c0_g1_i XP_026484035.1 beclin-1-like XP_026484035.1 13037.EHJ78273 1.p1 protein [Vanessa tameamea] tr|A0A212FCP0|A0A212FCP0_DA NPL Small G protein ras TRINITY_DN21311_c0_g1 tr|A0A212FCP0|A0A212FCP0_DA OS=Danaus plexippus plexippus 34740.HMEL010545-PA _i1.p1 NPL OX=278856 GN=KGM_215072 PE=4 SV=1 91

tr|A0A3S2LNM3|A0A3S2LNM3_C TRINITY_DN25919_c0_g1 tr|A0A3S2LNM3|A0A3S2LNM3_C HISP Uncharacterized protein 34740.HMEL013671-PA _i1.p1 HISP OS=Chilo suppressalis OX=168631 GN=evm_004791 PE=4 SV=1 tr|B0LD01|B0LD01_DANPL Slimb TRINITY_DN1834_c0_g1_i tr|B0LD01|B0LD01_DANPL OS=Danaus plexippus OX=13037 13037.EHJ76912 1.p1 GN=slimb PE=2 SV=1 tr|A0A1C9EGJ2|A0A1C9EGJ2_BI TRINITY_DN1562_c0_g1_i tr|A0A1C9EGJ2|A0A1C9EGJ2_BI CAN Integrin alpha-PS2 13037.EHJ68673 1.p1 CAN OS=Bicyclus anynana OX=110368 PE=3 SV=1 tr|A0A2W1BL66|A0A2W1BL66_H ELAM Uncharacterized protein TRINITY_DN20053_c0_g1 tr|A0A2W1BL66|A0A2W1BL66_H OS=Helicoverpa armigera 34740.HMEL018030-PA _i15.p1 ELAM OX=29058 GN=HaOG209491 PE=4 SV=1 TRINITY_DN1862_c0_g1_i XP_028166489.1 dynactin subunit XP_028166489.1 13037.EHJ74738 23.p1 1 [Ostrinia furnacalis] tr|A0A2H1WKE4|A0A2H1WKE4_ SPOFR SFRICE_007575 TRINITY_DN14003_c0_g1 tr|A0A2H1WKE4|A0A2H1WKE4_ 7091.BGIBMGA012295- OS=Spodoptera frugiperda _i1.p1 SPOFR TA OX=7108 GN=SFRICE_007575 PE=4 SV=1 tr|A0A437BNY3|A0A437BNY3_C HISP Defective in cullin TRINITY_DN3827_c0_g1_i tr|A0A437BNY3|A0A437BNY3_C neddylation protein OS=Chilo 34740.HMEL007416-PA 1.p2 HISP suppressalis OX=168631 GN=evm_003360 PE=4 SV=1 tr|S4PZQ9|S4PZQ9_9NEOP DNA- TRINITY_DN4523_c0_g1_i directed RNA polymerase III tr|S4PZQ9|S4PZQ9_9NEOP 34740.HMEL014869-PA 14.p4 OS=Pararge aegeria OX=116150 PE=4 SV=1 XP_026496751.1 mannosyl- TRINITY_DN2074_c0_g1_i oligosaccharide 1,2-alpha- XP_026496751.1 13037.EHJ63518 2.p1 mannosidase IA [Vanessa tameamea] tr|A0A212FM20|A0A212FM20_D ANPL Ubiquitin-like protein TRINITY_DN15067_c0_g1 tr|A0A212FM20|A0A212FM20_D ATG12 OS=Danaus plexippus 13037.EHJ78946 _i1.p2 ANPL plexippus OX=278856 GN=KGM_201156 PE=3 SV=1 TRINITY_DN13149_c0_g1 13037.EHJ73373 _i3.p1 XP_031768487.1 TRINITY_DN15328_c0_g2 phosphatidylinositol 5-phosphate 4- 7091.BGIBMGA001594- XP_031768487.1 _i1.p1 kinase type-2 alpha-like [Galleria TA mellonella] GBP68373.1 Ras-related C3 TRINITY_DN11927_c0_g1 GBP68373.1 botulinum toxin substrate 1 [Eumeta 13037.EHJ77267 _i1.p1 japonica] tr|A0A212ELQ2|A0A212ELQ2_D ANPL Erlin-1 like protein TRINITY_DN4127_c0_g1_i tr|A0A212ELQ2|A0A212ELQ2_D OS=Danaus plexippus plexippus 13037.EHJ65120 14.p1 ANPL OX=278856 GN=KGM_209743 PE=4 SV=1 TRINITY_DN1451_c0_g2_i XP_026483172.1 serine protease XP_026483172.1 34740.HMEL022597-PA 15.p1 snake-like [Vanessa tameamea] 92

tr|A0A212FMI7|A0A212FMI7_DA TRINITY_DN1570_c4_g1_i tr|A0A212FMI7|A0A212FMI7_DA NPL Chimerin OS=Danaus 13037.EHJ79050 8.p1 NPL plexippus plexippus OX=278856 GN=KGM_206933 PE=4 SV=1 XP_026483719.1 guanine TRINITY_DN1202_c0_g1_i XP_026483719.1 nucleotide-binding protein G(f) 13037.EHJ66725 5.p1 subunit alpha [Vanessa tameamea] XP_026486456.1 apoptosis- TRINITY_DN12952_c0_g1 XP_026486456.1 resistant E3 ubiquitin protein ligase 13037.EHJ69238 _i23.p1 1 isoform X1 [Vanessa tameamea] tr|A0A194Q8R3|A0A194Q8R3_PA PXU Ets DNA-binding protein TRINITY_DN1453_c4_g1_i tr|A0A194Q8R3|A0A194Q8R3_PA pokkuri OS=Papilio xuthus 34740.HMEL016694-PA 2.p1 PXU OX=66420 GN=RR46_05127 PE=3 SV=1 XP_026485298.1 autophagy-related TRINITY_DN7159_c0_g1_i XP_026485298.1 protein 16-1 isoform X2 [Vanessa 13037.EHJ73902 2.p1 tameamea] XP_028167948.1 uncharacterized TRINITY_DN10529_c0_g1 7091.BGIBMGA011034- XP_028167948.1 protein LOC114358248 [Ostrinia _i1.p2 TA furnacalis] XP_026492853.1 protein spaetzle- TRINITY_DN2802_c0_g1_i XP_026492853.1 like isoform X3 [Vanessa 13037.EHJ70909 2.p1 tameamea] tr|A0A212EXC2|A0A212EXC2_D ANPL Cell division cycle protein 16 TRINITY_DN3140_c0_g2_i tr|A0A212EXC2|A0A212EXC2_D OS=Danaus plexippus plexippus 13037.EHJ69420 3.p1 ANPL OX=278856 GN=KGM_205550 PE=4 SV=1 UniRef90_A0A1B6FVQ1 Protein kinase domain-containing protein TRINITY_DN15258_c0_g1 UniRef90_A0A1B6FVQ1 (Fragment) n=1 Tax=Cuerna arida 13037.EHJ65074 _i6.p1 TaxID=1464854 RepID=A0A1B6FVQ1_9HEMI UniRef90_A0A430QE12 Uncharacterized protein n=1 TRINITY_DN10028_c0_g1 UniRef90_A0A430QE12 Tax=Schistosoma bovis 34740.HMEL017572-PA _i2.p2 TaxID=6184 RepID=A0A430QE12_SCHBO TRINITY_DN1664_c0_g3_i 34740.HMEL008650-PA 1.p2 TRINITY_DN2025_c1_g1_i 34740.HMEL014143-PA 26.p1 TRINITY_DN3267_c2_g1_i XP_026497720.1 gloverin-like 7091.BGIBMGA013865- XP_026497720.1 15.p1 [Vanessa tameamea] TA XP_026489504.1 protein TRINITY_DN13543_c1_g1 XP_026489504.1 croquemort-like isoform X1 34740.HMEL009291-PA _i12.p1 [Vanessa tameamea] tr|A0A212FAG9|A0A212FAG9_D ANPL Tubulointerstitial nephritis TRINITY_DN1024_c0_g1_i tr|A0A212FAG9|A0A212FAG9_D antigen OS=Danaus plexippus 13037.EHJ74345 13.p1 ANPL plexippus OX=278856 GN=KGM_209867 PE=3 SV=1 XP_026488057.1 PAX-interacting TRINITY_DN2696_c0_g2_i XP_026488057.1 protein 1 isoform X1 [Vanessa 13037.EHJ79047 3.p1 tameamea] 93

XP_028042571.1 inducible TRINITY_DN14669_c0_g1 XP_028042571.1 metalloproteinase inhibitor protein- 34740.HMEL006528-PA _i2.p1 like, partial [Bombyx mandarina] TRINITY_DN6726_c1_g1_i RVE52843.1 hypothetical protein RVE52843.1 13037.EHJ63159 4.p1 evm_002500 [Chilo suppressalis] tr|A0A212FBE1|A0A212FBE1_DA NPL Rho-related BTB domain- TRINITY_DN48466_c0_g1 tr|A0A212FBE1|A0A212FBE1_DA containing protein 2 OS=Danaus 13037.EHJ74706 _i2.p1 NPL plexippus plexippus OX=278856 GN=KGM_210262 PE=4 SV=1 tr|A0A2A4K8M1|A0A2A4K8M1_ HELVI CULLIN_2 domain- TRINITY_DN4746_c0_g2_i tr|A0A2A4K8M1|A0A2A4K8M1_ 7091.BGIBMGA005758- containing protein OS=Heliothis 2.p1 HELVI TA virescens OX=7102 GN=B5V51_8976 PE=3 SV=1 TRINITY_DN21043_c0_g1 KPJ02437.1 Protein toll [Papilio 7091.BGIBMGA006244- KPJ02437.1 _i2.p1 xuthus] TA tr|A0A0F6NDA9|A0A0F6NDA9_O TRINITY_DN11574_c0_g1 tr|A0A0F6NDA9|A0A0F6NDA9_O STNU Dscam (Fragment) 34740.HMEL007149-PA _i11.p2 STNU OS=Ostrinia nubilalis OX=29057 GN=dscam PE=4 SV=1 tr|A0A2A4J5I1|A0A2A4J5I1_HEL TRINITY_DN14280_c0_g1 tr|A0A2A4J5I1|A0A2A4J5I1_HEL VI Ribosomal protein S6 kinase 34740.HMEL005748-PA _i11.p1 VI OS=Heliothis virescens OX=7102 GN=B5V51_7378 PE=3 SV=1 tr|A0A5E4Q7S2|A0A5E4Q7S2_9N EOP Cytochrome b5 heme-binding TRINITY_DN9895_c0_g1_i tr|A0A5E4Q7S2|A0A5E4Q7S2_9N domain-containing protein 34740.HMEL010184-PA 8.p1 EOP OS=Leptidea sinapis OX=189913 GN=LSINAPIS_LOCUS5605 PE=3 SV=1 tr|A0A5E4QDS9|A0A5E4QDS9_9 NEOP TNF_2 domain-containing TRINITY_DN10270_c0_g1 tr|A0A5E4QDS9|A0A5E4QDS9_9 protein OS=Leptidea sinapis 34740.HMEL004073-PA _i1.p1 NEOP OX=189913 GN=LSINAPIS_LOCUS6992 PE=3 SV=1 tr|S4PFQ8|S4PFQ8_9NEOP TRINITY_DN5718_c1_g1_i tr|S4PFQ8|S4PFQ8_9NEOP Galectin OS=Pararge aegeria 34740.HMEL005121-PA 7.p1 OX=116150 PE=4 SV=1 TRINITY_DN37702_c1_g1 KOB76811.1 putative tick KOB76811.1 34740.HMEL013671-PA _i1.p1 transposon [Operophtera brumata] tr|S4PYH1|S4PYH1_9NEOP Lola TRINITY_DN7712_c0_g1_i tr|S4PYH1|S4PYH1_9NEOP (Fragment) OS=Pararge aegeria 34740.HMEL005659-PA 2.p1 OX=116150 PE=4 SV=1 XP_004921521.2 inducible TRINITY_DN14669_c0_g1 XP_004921521.2 metalloproteinase inhibitor protein- 13037.EHJ63159 _i1.p1 like, partial [Bombyx mori] tr|A0A2H1VJN0|A0A2H1VJN0_SP OFR SFRICE_005082 TRINITY_DN3354_c0_g1_i tr|A0A2H1VJN0|A0A2H1VJN0_SP OS=Spodoptera frugiperda 34740.HMEL005347-PA 8.p1 OFR OX=7108 GN=SFRICE_005082 PE=4 SV=1 TRINITY_DN1296_c0_g1_i 34740.HMEL002834-PA 13.p3 XP_026483072.1 inactive peptidyl- TRINITY_DN28376_c0_g1 XP_026483072.1 prolyl cis-trans isomerase 34740.HMEL011521-PA _i23.p1 shutdown-like [Vanessa tameamea] 94

tr|A0A2H1VA96|A0A2H1VA96_SP OFR Autophagy-related protein 9 TRINITY_DN10151_c0_g1 tr|A0A2H1VA96|A0A2H1VA96_SP OS=Spodoptera frugiperda 34740.HMEL008818-PA _i1.p1 OFR OX=7108 GN=SFRICE_002409 PE=3 SV=1 tr|A0A140KFU4|A0A140KFU4_B TRINITY_DN3907_c0_g1_i tr|A0A140KFU4|A0A140KFU4_B OMMO Ets transcription factor 34740.HMEL004734-PA 3.p1 OMMO OS=Bombyx mori OX=7091 GN=pointed2 PE=2 SV=1 tr|A0A3S2TRY5|A0A3S2TRY5_C TRINITY_DN5182_c0_g1_i tr|A0A3S2TRY5|A0A3S2TRY5_C HISP Uncharacterized protein 34740.HMEL015851-PA 2.p1 HISP OS=Chilo suppressalis OX=168631 GN=evm_001875 PE=4 SV=1 TRINITY_DN1862_c0_g1_i XP_030034268.1 dynactin subunit XP_030034268.1 13037.EHJ74738 17.p1 1 [Manduca sexta] tr|A0A2A4IXN3|A0A2A4IXN3_H ELVI Nudix hydrolase domain- TRINITY_DN10028_c0_g1 tr|A0A2A4IXN3|A0A2A4IXN3_H containing protein OS=Heliothis 34740.HMEL017572-PA _i1.p1 ELVI virescens OX=7102 GN=B5V51_11387 PE=4 SV=1 tr|B2DBJ5|B2DBJ5_PAPXU DEAD TRINITY_DN403_c1_g2_i7 tr|B2DBJ5|B2DBJ5_PAPXU box polypeptide 5 OS=Papilio 34740.HMEL015763-PA .p1 xuthus OX=66420 PE=2 SV=1 XP_026488152.1 SPRY domain- TRINITY_DN10601_c0_g1 XP_026488152.1 containing SOCS box protein 3-like 34740.HMEL012348-PA _i4.p1 isoform X2 [Vanessa tameamea] XP_026496596.1 SHC- TRINITY_DN3610_c0_g1_i XP_026496596.1 transforming protein 1 [Vanessa 13037.EHJ69724 14.p1 tameamea] XP_026485623.1 ankyrin repeat TRINITY_DN1288_c1_g1_i XP_026485623.1 domain-containing protein 17 13037.EHJ73802 41.p1 [Vanessa tameamea] tr|A0A212F957|A0A212F957_DA NPL Histone acetyltransferase TRINITY_DN5460_c0_g1_i tr|A0A212F957|A0A212F957_DA OS=Danaus plexippus plexippus 13037.EHJ73824 8.p1 NPL OX=278856 GN=KGM_213223 PE=3 SV=1 XP_026496519.1 ran-binding TRINITY_DN4374_c0_g1_i XP_026496519.1 protein 9 isoform X3 [Vanessa 13037.EHJ64432 2.p1 tameamea] tr|A0A2H1VAP3|A0A2H1VAP3_S POFR SFRICE_001720 TRINITY_DN5497_c0_g1_i tr|A0A2H1VAP3|A0A2H1VAP3_S OS=Spodoptera frugiperda 34740.HMEL012894-PA 1.p1 POFR OX=7108 GN=SFRICE_001720 PE=3 SV=1 tr|S4PGW7|S4PGW7_9NEOP TRINITY_DN107_c0_g1_i1 Interleukin enhancer binding factor tr|S4PGW7|S4PGW7_9NEOP 34740.HMEL005228-PA 2.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 TRINITY_DN3188_c4_g1_i XP_026497749.1 gloverin-like XP_026497749.1 13037.EHJ66058 1.p1 [Vanessa tameamea] tr|A0A212F5F3|A0A212F5F3_DA TRINITY_DN1273_c1_g2_i tr|A0A212F5F3|A0A212F5F3_DA NPL Cytochrome b5 OS=Danaus 13037.EHJ72442 1.p1 NPL plexippus plexippus OX=278856 GN=KGM_215294 PE=3 SV=1 XP_026496518.1 ran-binding TRINITY_DN6365_c0_g1_i XP_026496518.1 protein 9 isoform X2 [Vanessa 13037.EHJ64432 1.p1 tameamea] 95

XP_028159201.1 LOW QUALITY TRINITY_DN14145_c0_g1 XP_028159201.1 PROTEIN: lebocin-4-like [Ostrinia 13037.EHJ64534 _i2.p1 furnacalis] XP_026489801.1 putative inhibitor TRINITY_DN1619_c0_g1_i XP_026489801.1 of apoptosis isoform X1 [Vanessa 34740.HMEL002564-PA 7.p1 tameamea] tr|A0A212F470|A0A212F470_DA TRINITY_DN6503_c0_g1_i tr|A0A212F470|A0A212F470_DA NPL Pannier OS=Danaus plexippus 13037.EHJ71973 9.p1 NPL plexippus OX=278856 GN=KGM_211454 PE=4 SV=1 XP_026494275.1 CLIP domain- TRINITY_DN1853_c0_g1_i XP_026494275.1 containing serine protease 2-like 13037.EHJ73834 17.p1 isoform X1 [Vanessa tameamea] TRINITY_DN1743_c0_g1_i XP_026485435.1 protein lap4-like XP_026485435.1 13037.EHJ69387 2.p1 isoform X8 [Vanessa tameamea] tr|C9DTM4|C9DTM4_BOMMO TRINITY_DN1298_c0_g2_i 7091.BGIBMGA012467- tr|C9DTM4|C9DTM4_BOMMO CAP isoform A OS=Bombyx mori 4.p2 TA OX=7091 PE=2 SV=1 TRINITY_DN2490_c0_g2_i VVD03335.1 unnamed protein VVD03335.1 13037.EHJ68989 2.p1 product [Leptidea sinapis] tr|S4NYG7|S4NYG7_9NEOP TRINITY_DN12611_c0_g1 Condensin complex subunit 1 tr|S4NYG7|S4NYG7_9NEOP 34740.HMEL017572-PA _i3.p2 (Fragment) OS=Pararge aegeria OX=116150 PE=4 SV=1 tr|A0A5E4Q240|A0A5E4Q240_9N EOP TEA domain-containing TRINITY_DN1832_c2_g1_i tr|A0A5E4Q240|A0A5E4Q240_9N protein (Fragment) OS=Leptidea 13037.EHJ63836 1.p1 EOP sinapis OX=189913 GN=LSINAPIS_LOCUS4336 PE=4 SV=1 UniRef90_A0A1E1WJX5 Uncharacterized protein (Fragment) TRINITY_DN3_c33_g1_i1. UniRef90_A0A1E1WJX5 n=1 Tax=Pectinophora gossypiella 34740.HMEL018030-PA p1 TaxID=13191 RepID=A0A1E1WJX5_PECGO TRINITY_DN0_c23_g1_i4. XP_026501750.1 hemicentin-1-like XP_026501750.1 13037.EHJ71735 p1 [Vanessa tameamea] XP_013141759.1 PREDICTED: TRINITY_DN133151_c0_g 7091.BGIBMGA001303- XP_013141759.1 uncharacterized protein 1_i1.p1 TA LOC106105849 [Papilio polytes] XP_026495057.1 LOW QUALITY TRINITY_DN17299_c0_g1 XP_026495057.1 PROTEIN: protein phosphatase 13037.EHJ63863 _i5.p1 Slingshot [Vanessa tameamea] tr|I4DLF7|I4DLF7_PAPXU Clip- TRINITY_DN7027_c2_g1_i domain serine protease, family C tr|I4DLF7|I4DLF7_PAPXU 13037.EHJ63474 1.p1 OS=Papilio xuthus OX=66420 PE=2 SV=1 TRINITY_DN1095_c1_g1_i XP_026490329.1 protein kibra XP_026490329.1 13037.EHJ73876 15.p1 isoform X2 [Vanessa tameamea] TRINITY_DN17299_c0_g1 13037.EHJ63863 _i15.p4 tr|A0A212EW16|A0A212EW16_D ANPL C-type lectin domain- TRINITY_DN5575_c0_g1_i tr|A0A212EW16|A0A212EW16_D containing protein OS=Danaus 13037.EHJ68817 1.p1 ANPL plexippus plexippus OX=278856 GN=KGM_210065 PE=4 SV=1 96

XP_026488800.1 RNA TRINITY_DN14646_c0_g1 pseudouridylate synthase domain- 7091.BGIBMGA006020- XP_026488800.1 _i23.p1 containing protein 2-like isoform TA X3 [Vanessa tameamea] XP_026494481.1 microtubule- TRINITY_DN1462_c1_g1_i associated protein RP/EB family 7091.BGIBMGA004038- XP_026494481.1 2.p1 member 1 isoform X2 [Vanessa TA tameamea] tr|A0A212FC61|A0A212FC61_DA NPL Apoptosis-inducing factor 1 TRINITY_DN2327_c0_g3_i tr|A0A212FC61|A0A212FC61_DA OS=Danaus plexippus plexippus 13037.EHJ74960 6.p1 NPL OX=278856 GN=KGM_204643 PE=4 SV=1 TRINITY_DN6726_c0_g1_i 13037.EHJ63159 1.p4 tr|A0A212FGP7|A0A212FGP7_DA NPL Mitogen-activated protein TRINITY_DN4957_c0_g1_i tr|A0A212FGP7|A0A212FGP7_DA kinase kinase kinase 4 OS=Danaus 13037.EHJ76732 2.p1 NPL plexippus plexippus OX=278856 GN=KGM_213875 PE=4 SV=1 XP_022830336.1 longitudinals TRINITY_DN3527_c0_g1_i XP_022830336.1 lacking protein, isoforms A/B/D/L- 34740.HMEL005659-PA 37.p1 like isoform X5 [Spodoptera litura] tr|A0A194QNW3|A0A194QNW3_P APXU Serine protease HTRA2, TRINITY_DN10067_c0_g1 tr|A0A194QNW3|A0A194QNW3_P 7091.BGIBMGA002318- mitochondrial OS=Papilio xuthus _i4.p1 APXU TA OX=66420 GN=RR46_04008 PE=4 SV=1 tr|A0A212F470|A0A212F470_DA TRINITY_DN2500_c0_g3_i tr|A0A212F470|A0A212F470_DA NPL Pannier OS=Danaus plexippus 13037.EHJ71973 1.p1 NPL plexippus OX=278856 GN=KGM_211454 PE=4 SV=1 UniRef90_A0A443QZM3 Hemicentin-2-like protein TRINITY_DN147375_c0_g (Fragment) n=2 UniRef90_A0A443QZM3 13037.EHJ74080 1_i1.p1 Tax=Dinothrombium tinctorium TaxID=1965070 RepID=A0A443QZM3_9ACAR UniRef90_A0A1B6E4Q0 Uncharacterized protein (Fragment) TRINITY_DN110199_c0_g UniRef90_A0A1B6E4Q0 n=1 Tax=Clastoptera arizonana 34740.HMEL010384-PA 1_i1.p1 TaxID=38151 RepID=A0A1B6E4Q0_9HEMI XP_026485623.1 ankyrin repeat TRINITY_DN1288_c1_g1_i XP_026485623.1 domain-containing protein 17 13037.EHJ73802 25.p1 [Vanessa tameamea] XP_026498695.1 enhancer of TRINITY_DN6485_c0_g2_i XP_026498695.1 mRNA-decapping protein 4-like, 34740.HMEL012843-PA 1.p1 partial [Vanessa tameamea] tr|A0A194R7A1|A0A194R7A1_PA TRINITY_DN9055_c0_g2_i tr|A0A194R7A1|A0A194R7A1_PA PMA Serpin B10 OS=Papilio 13037.EHJ65045 1.p1 PMA machaon OX=76193 GN=RR48_10716 PE=3 SV=1 TRINITY_DN10653_c0_g1 XP_026485746.1 alaserpin-like XP_026485746.1 13037.EHJ75277 _i7.p1 isoform X2 [Vanessa tameamea] 97

tr|A0A2H1WH37|A0A2H1WH37_ SPOFR SFRICE_041254 TRINITY_DN10155_c0_g1 tr|A0A2H1WH37|A0A2H1WH37_ OS=Spodoptera frugiperda 34740.HMEL016226-PA _i21.p1 SPOFR OX=7108 GN=SFRICE_041254 PE=4 SV=1 TRINITY_DN1570_c4_g1_i VVC97867.1 unnamed protein 7091.BGIBMGA012914- VVC97867.1 4.p1 product [Leptidea sinapis] TA XP_022223383.1 beta-2 adrenergic TRINITY_DN130833_c0_g XP_022223383.1 receptor isoform X2 [Drosophila 34740.HMEL025046-PA 1_i1.p1 obscura] TRINITY_DN3409_c0_g1_i GBP30764.1 Regulatory-associated GBP30764.1 13037.EHJ76100 10.p2 protein of mTOR [Eumeta japonica] tr|A0A212ERI4|A0A212ERI4_DA NPL Caudal like protein (Fragment) TRINITY_DN17036_c0_g1 tr|A0A212ERI4|A0A212ERI4_DA OS=Danaus plexippus plexippus 13037.EHJ66999 _i2.p2 NPL OX=278856 GN=KGM_215988A PE=4 SV=1 XP_026483229.1 ras-related protein TRINITY_DN3443_c0_g2_i XP_026483229.1 Ral-a isoform X2 [Vanessa 13037.EHJ75874 8.p1 tameamea] XP_026493994.1 apoptosis- TRINITY_DN6822_c0_g1_i XP_026493994.1 stimulating of p53 protein 2 isoform 13037.EHJ73745 1.p1 X5 [Vanessa tameamea] RXG52210.1 Hemocyte protein- TRINITY_DN152481_c0_g glutamine gamma- RXG52210.1 34740.HMEL012424-PA 1_i1.p1 glutamyltransferase [Armadillidium vulgare] tr|Q8WSA1|Q8WSA1_BOMMO TRINITY_DN16220_c0_g1 Orphan nuclear receptor E75C tr|Q8WSA1|Q8WSA1_BOMMO 34740.HMEL014074-PA _i3.p1 OS=Bombyx mori OX=7091 GN=E75 PE=2 SV=1 TRINITY_DN3031_c0_g1_i XP_026484380.1 protein sickie XP_026484380.1 13037.EHJ78388 10.p1 isoform X5 [Vanessa tameamea] XP_026491088.1 vascular TRINITY_DN192_c3_g2_i2 XP_026491088.1 endothelial growth factor receptor 1 13037.EHJ78656 .p1 [Vanessa tameamea] XP_026491003.1 inward rectifier TRINITY_DN6863_c0_g1_i XP_026491003.1 potassium channel 4-like [Vanessa 34740.HMEL004824-PA 1.p1 tameamea] tr|J9XR17|J9XR17_SPOLT TRINITY_DN1043_c0_g1_i Autophagy-related protein 3 tr|J9XR17|J9XR17_SPOLT 34740.HMEL003955-PA 12.p1 OS=Spodoptera litura OX=69820 PE=2 SV=1 XP_022124098.1 uncharacterized TRINITY_DN22558_c0_g1 XP_022124098.1 protein LOC110999383 [Pieris 13037.EHJ73692 _i17.p2 rapae] tr|S4P9U3|S4P9U3_9NEOP Serine TRINITY_DN3333_c0_g1_i tr|S4P9U3|S4P9U3_9NEOP protease inhibitor 3 OS=Pararge 13037.EHJ73921 2.p1 aegeria OX=116150 PE=3 SV=1 tr|A0A2A4JI47|A0A2A4JI47_HEL VI PI3K/PI4K domain-containing TRINITY_DN12294_c0_g1 tr|A0A2A4JI47|A0A2A4JI47_HEL protein (Fragment) OS=Heliothis 34740.HMEL022673-PA _i1.p1 VI virescens OX=7102 GN=B5V51_1536 PE=3 SV=1 98

tr|A0A194QCQ5|A0A194QCQ5_P APXU Alcohol dehydrogenase TRINITY_DN19036_c0_g1 tr|A0A194QCQ5|A0A194QCQ5_P [NADP+] OS=Papilio xuthus 13037.EHJ72113 _i2.p1 APXU OX=66420 GN=RR46_09968 PE=4 SV=1 tr|A0A2A4J1G8|A0A2A4J1G8_HE LVI Mitogen-activated protein TRINITY_DN1093_c3_g1_i tr|A0A2A4J1G8|A0A2A4J1G8_HE kinase OS=Heliothis virescens 34740.HMEL005541-PA 1.p1 LVI OX=7102 GN=B5V51_8574 PE=4 SV=1 XP_026498429.1 heterogeneous TRINITY_DN1746_c0_g1_i XP_026498429.1 nuclear ribonucleoprotein H2-like 34740.HMEL008154-PA 14.p1 isoform X1 [Vanessa tameamea] UniRef90_A0A1E1WNZ7 Uncharacterized protein (Fragment) TRINITY_DN154776_c0_g UniRef90_A0A1E1WNZ7 n=1 Tax=Pectinophora gossypiella 34740.HMEL013671-PA 1_i1.p1 TaxID=13191 RepID=A0A1E1WNZ7_PECGO tr|A0A437B0L5|A0A437B0L5_CH ISP G_PROTEIN_RECEP_F1_2 TRINITY_DN20056_c0_g1 tr|A0A437B0L5|A0A437B0L5_CH domain-containing protein 34740.HMEL025046-PA _i2.p1 ISP OS=Chilo suppressalis OX=168631 GN=evm_011406 PE=4 SV=1 tr|A0A0R4IPD4|A0A0R4IPD4_DA NRE Down syndrome cell adhesion TRINITY_DN1715_c1_g1_i tr|A0A0R4IPD4|A0A0R4IPD4_DA molecule-like 1 (Fragment) 7070.TC012539-PA 45.p2 NRE OS=Danio rerio OX=7955 GN=dscaml1 PE=4 SV=1 tr|A0A194PNW9|A0A194PNW9_P APXU Wilms tumor protein 1- TRINITY_DN10927_c0_g1 tr|A0A194PNW9|A0A194PNW9_P 7091.BGIBMGA003146- interacting protein-like OS=Papilio _i1.p1 APXU TA xuthus OX=66420 GN=RR46_05930 PE=4 SV=1 TRINITY_DN443_c0_g1_i1 XP_026487589.1 CD109 antigen- XP_026487589.1 13037.EHJ64472 .p1 like [Vanessa tameamea] tr|A0A212F5H5|A0A212F5H5_DA NPL Apoptosis-inducing factor TRINITY_DN5226_c0_g1_i tr|A0A212F5H5|A0A212F5H5_DA OS=Danaus plexippus plexippus 34740.HMEL014727-PA 3.p1 NPL OX=278856 GN=KGM_211545 PE=4 SV=1 TRINITY_DN6663_c0_g1_i 13037.EHJ76100 6.p2 tr|A0A0F6NCU9|A0A0F6NCU9_O TRINITY_DN11574_c0_g1 tr|A0A0F6NCU9|A0A0F6NCU9_O STNU Dscam (Fragment) 34740.HMEL007149-PA _i20.p1 STNU OS=Ostrinia nubilalis OX=29057 GN=dscam PE=4 SV=1 tr|A0A2A4JJ01|A0A2A4JJ01_HEL TRINITY_DN8148_c2_g1_i tr|A0A2A4JJ01|A0A2A4JJ01_HEL VI Uncharacterized protein 7091.BGIBMGA000603- 3.p1 VI OS=Heliothis virescens OX=7102 TA GN=B5V51_1185 PE=4 SV=1 tr|A0A212FLJ2|A0A212FLJ2_DA NPL MARVEL domain-containing TRINITY_DN17769_c0_g1 tr|A0A212FLJ2|A0A212FLJ2_DA protein (Fragment) OS=Danaus 13037.EHJ78735 _i4.p1 NPL plexippus plexippus OX=278856 GN=KGM_206087B PE=4 SV=1 XP_026500696.1 autophagy-related TRINITY_DN10240_c0_g2 XP_026500696.1 protein 13 homolog [Vanessa 34740.HMEL014081-PA _i4.p1 tameamea] 99

tr|A0A5E4R483|A0A5E4R483_9N EOP VHS domain-containing TRINITY_DN3856_c0_g1_i tr|A0A5E4R483|A0A5E4R483_9N protein OS=Leptidea sinapis 13037.EHJ69303 5.p2 EOP OX=189913 GN=LSINAPIS_LOCUS14899 PE=4 SV=1 tr|A0A212F5K0|A0A212F5K0_DA NPL Mediator of RNA polymerase TRINITY_DN215_c0_g1_i7 tr|A0A212F5K0|A0A212F5K0_DA 2 transcription subunit 25 34740.HMEL004110-PA 6.p1 NPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_203515 PE=4 SV=1 tr|A0A212EI97|A0A212EI97_DAN PL SNF4/AMP-activated protein TRINITY_DN7380_c0_g1_i tr|A0A212EI97|A0A212EI97_DAN kinase gamma subunit OS=Danaus 13037.EHJ63782 7.p1 PL plexippus plexippus OX=278856 GN=KGM_212579 PE=4 SV=1 XP_026485623.1 ankyrin repeat TRINITY_DN1288_c1_g1_i XP_026485623.1 domain-containing protein 17 13037.EHJ73802 28.p1 [Vanessa tameamea] TRINITY_DN2039_c0_g1_i 13037.EHJ65548 3.p2 TRINITY_DN13277_c0_g1 XP_026494958.1 caspase-1-like XP_026494958.1 34740.HMEL004700-PA _i1.p1 [Vanessa tameamea] tr|A0A194RAV9|A0A194RAV9_PA TRINITY_DN12174_c0_g1 tr|A0A194RAV9|A0A194RAV9_PA PMA Protein toll OS=Papilio 7091.BGIBMGA011084- _i1.p1 PMA machaon OX=76193 TA GN=RR48_13491 PE=4 SV=1 TRINITY_DN10653_c0_g1 XP_026485746.1 alaserpin-like XP_026485746.1 13037.EHJ75277 _i18.p1 isoform X2 [Vanessa tameamea] TRINITY_DN2992_c0_g1_i XP_026495999.1 lysozyme-like XP_026495999.1 34740.HMEL016024-PA 1.p1 isoform X1 [Vanessa tameamea] TRINITY_DN7787_c0_g1_i XP_021193311.1 protein toll-like XP_021193311.1 34740.HMEL010927-PA 2.p1 [Helicoverpa armigera] tr|A0A212FF76|A0A212FF76_DA NPL Tuberin like protein TRINITY_DN6895_c1_g2_i tr|A0A212FF76|A0A212FF76_DA OS=Danaus plexippus plexippus 13037.EHJ76090 8.p1 NPL OX=278856 GN=KGM_209357 PE=4 SV=1 tr|A0A212ENQ6|A0A212ENQ6_D ANPL Dynactin subunit 3 TRINITY_DN10342_c0_g1 tr|A0A212ENQ6|A0A212ENQ6_D OS=Danaus plexippus plexippus 34740.HMEL015241-PA _i1.p1 ANPL OX=278856 GN=KGM_208702 PE=4 SV=1 tr|A0A212EQK0|A0A212EQK0_D ANPL Staphylococcal nuclease TRINITY_DN13808_c0_g1 tr|A0A212EQK0|A0A212EQK0_D domain-containing protein 13037.EHJ66647 _i1.p1 ANPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_203403 PE=4 SV=1 tr|A0A3S2NTQ8|A0A3S2NTQ8_C HISP Paired domain-containing TRINITY_DN24063_c0_g1 tr|A0A3S2NTQ8|A0A3S2NTQ8_C protein OS=Chilo suppressalis 34740.HMEL003749-PA _i3.p1 HISP OX=168631 GN=evm_007003 PE=4 SV=1 tr|H3C8W0|H3C8W0_TETNG TRINITY_DN2696_c0_g1_i Uncharacterized protein tr|H3C8W0|H3C8W0_TETNG 34740.HMEL008670-PA 3.p2 OS=Tetraodon nigroviridis OX=99883 PE=4 SV=1 100

UniRef90_A0A1Y1MDB4 Uncharacterized protein (Fragment) TRINITY_DN24487_c0_g2 UniRef90_A0A1Y1MDB4 n=1 Tax=Photinus pyralis 13037.EHJ77866 _i6.p1 TaxID=7054 RepID=A0A1Y1MDB4_PHOPY XP_021199926.1 leucine-rich TRINITY_DN763_c0_g1_i1 repeat serine/threonine-protein XP_021199926.1 13037.EHJ76914 2.p2 kinase 1-like [Helicoverpa armigera] tr|S4PWM8|S4PWM8_9NEOP TRINITY_DN3583_c0_g1_i tr|S4PWM8|S4PWM8_9NEOP Protein charybde OS=Pararge 34740.HMEL010294-PA 1.p1 aegeria OX=116150 PE=4 SV=1 tr|A0A194QFR3|A0A194QFR3_PA PXU Fragile X mental retardation TRINITY_DN3637_c1_g3_i tr|A0A194QFR3|A0A194QFR3_PA syndrome-related protein 1 13037.EHJ73477 2.p1 PXU OS=Papilio xuthus OX=66420 GN=RR46_01752 PE=4 SV=1 tr|A0A0N0PA48|A0A0N0PA48_PA TRINITY_DN1482_c0_g1_i tr|A0A0N0PA48|A0A0N0PA48_PA PXU Galectin OS=Papilio xuthus 34740.HMEL010163-PA 1.p1 PXU OX=66420 GN=RR46_01207 PE=4 SV=1 tr|A0A212FIG5|A0A212FIG5_DA NPL mRNA export protein TRINITY_DN5027_c0_g1_i tr|A0A212FIG5|A0A212FIG5_DA OS=Danaus plexippus plexippus 34740.HMEL008585-PA 4.p1 NPL OX=278856 GN=KGM_203528 PE=4 SV=1 TRINITY_DN5228_c3_g3_i AAA29331.1 serine protease AAA29331.1 13037.EHJ63957 1.p1 inhibitor, partial [Manduca sexta] XP_026492495.1 C-terminal- TRINITY_DN10336_c0_g1 7091.BGIBMGA002346- XP_026492495.1 binding protein isoform X1 _i4.p1 TA [Vanessa tameamea] tr|A0A0N1IMR0|A0A0N1IMR0_P TRINITY_DN565_c0_g1_i1 tr|A0A0N1IMR0|A0A0N1IMR0_P APXU Serpin B8 OS=Papilio 34740.HMEL007598-PA .p1 APXU xuthus OX=66420 GN=RR46_01513 PE=3 SV=1 XP_021197778.1 E3 ubiquitin- TRINITY_DN11762_c1_g1 XP_021197778.1 protein ligase highwire-like 13037.EHJ66655 _i1.p2 [Helicoverpa armigera] tr|A0A212FBJ8|A0A212FBJ8_DA NPL TAK1-associated binding TRINITY_DN4137_c0_g1_i tr|A0A212FBJ8|A0A212FBJ8_DA protein 2 isoform A OS=Danaus 13037.EHJ74734 5.p1 NPL plexippus plexippus OX=278856 GN=KGM_200977 PE=4 SV=1 tr|A0A194PHS7|A0A194PHS7_PA PXU STE20-related kinase adapter TRINITY_DN9000_c0_g1_i tr|A0A194PHS7|A0A194PHS7_PA protein alpha OS=Papilio xuthus 34740.HMEL009275-PA 29.p1 PXU OX=66420 GN=RR46_14192 PE=4 SV=1 UniRef90_R9W0H4 Dscam TRINITY_DN17986_c0_g1 (Fragment) n=1 Tax=Maladera UniRef90_R9W0H4 34740.HMEL007149-PA _i7.p1 orientalis TaxID=941252 RepID=R9W0H4_9SCAR XP_026495140.1 uncharacterized TRINITY_DN4683_c1_g1_i XP_026495140.1 protein LOC113400008 [Vanessa 13037.EHJ65343 7.p1 tameamea] tr|H9JMW8|H9JMW8_BOMMO TRINITY_DN113813_c0_g Spaetzle domain-containing protein tr|H9JMW8|H9JMW8_BOMMO 13037.EHJ66092 1_i1.p1 OS=Bombyx mori OX=7091 PE=4 SV=1 101

tr|A0A3S2LGS5|A0A3S2LGS5_C HISP HMG box domain-containing TRINITY_DN2519_c0_g1_i tr|A0A3S2LGS5|A0A3S2LGS5_C protein OS=Chilo suppressalis 13037.EHJ72465 45.p1 HISP OX=168631 GN=evm_001885 PE=4 SV=1 tr|A0A212FF76|A0A212FF76_DA NPL Tuberin like protein TRINITY_DN6895_c1_g2_i tr|A0A212FF76|A0A212FF76_DA OS=Danaus plexippus plexippus 13037.EHJ76090 14.p1 NPL OX=278856 GN=KGM_209357 PE=4 SV=1 XP_026488635.1 phenoloxidase- TRINITY_DN10597_c0_g1 XP_026488635.1 activating factor 2-like [Vanessa 34740.HMEL005224-PA _i1.p1 tameamea] XP_026496522.1 ran-binding TRINITY_DN4374_c0_g1_i XP_026496522.1 protein 9 isoform X5 [Vanessa 13037.EHJ64432 7.p1 tameamea] TRINITY_DN2543_c0_g1_i XP_026484236.1 ras suppressor XP_026484236.1 13037.EHJ64332 1.p2 protein 1 [Vanessa tameamea] XP_026500259.1 peptidoglycan TRINITY_DN2587_c1_g2_i 7091.BGIBMGA000586- XP_026500259.1 recognition protein-like isoform X1 2.p1 TA [Vanessa tameamea] tr|S4P9P2|S4P9P2_9NEOP TRINITY_DN10704_c0_g1 tr|S4P9P2|S4P9P2_9NEOP Cysteine protease OS=Pararge 13037.EHJ72670 _i2.p1 aegeria OX=116150 PE=3 SV=1 TRINITY_DN120_c0_g1_i1 XP_026486099.1 heat shock protein XP_026486099.1 13037.EHJ63492 .p2 26-like [Vanessa tameamea] tr|A0A212F7X6|A0A212F7X6_DA NPL Beclin 1-associated autophagy- TRINITY_DN13149_c0_g1 tr|A0A212F7X6|A0A212F7X6_DA related key regulator like protein 13037.EHJ73373 _i10.p1 NPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_201835 PE=4 SV=1 TRINITY_DN5452_c0_g1_i XP_028029524.1 eyes absent XP_028029524.1 13037.EHJ75518 16.p1 homolog 2 [Bombyx mandarina] XP_026494152.1 phenoloxidase- TRINITY_DN1682_c0_g2_i XP_026494152.1 activating factor 2-like [Vanessa 34740.HMEL013468-PA 8.p1 tameamea] TRINITY_DN29107_c0_g1 XP_026495706.1 lysozyme-like XP_026495706.1 34740.HMEL008220-PA _i5.p1 [Vanessa tameamea] XP_032514719.1 beta- TRINITY_DN11494_c0_g2 XP_032514719.1 ureidopropionase-like [Danaus 13037.EHJ70006 _i1.p2 plexippus plexippus] PZC85920.1 hypothetical protein TRINITY_DN1791_c2_g1_i PZC85920.1 B5X24_HaOG214285 [Helicoverpa 34740.HMEL017750-PA 1.p3 armigera] tr|A0A2H1V3R7|A0A2H1V3R7_S POFR SFRICE_003662 TRINITY_DN9882_c0_g1_i tr|A0A2H1V3R7|A0A2H1V3R7_S 7091.BGIBMGA003355- OS=Spodoptera frugiperda 3.p1 POFR TA OX=7108 GN=SFRICE_003662 PE=4 SV=1 tr|A0A194QU31|A0A194QU31_PA PMA Vacuolar fusion protein TRINITY_DN8231_c0_g1_i tr|A0A194QU31|A0A194QU31_PA CCZ1-like OS=Papilio machaon 13037.EHJ64890 1.p1 PMA OX=76193 GN=RR48_12253 PE=4 SV=1 102

tr|S4PK70|S4PK70_9NEOP CLIP TRINITY_DN9026_c0_g1_i domain-containing serine protease tr|S4PK70|S4PK70_9NEOP 34740.HMEL002402-PA 3.p1 OS=Pararge aegeria OX=116150 PE=3 SV=1 TRINITY_DN176_c2_g1_i4 XP_026485749.1 alaserpin-like XP_026485749.1 13037.EHJ75277 .p1 isoform X1 [Vanessa tameamea] TRINITY_DN1162_c1_g1_i XP_026497558.1 DE-cadherin XP_026497558.1 34740.HMEL008474-PA 2.p1 isoform X2 [Vanessa tameamea] XP_014362846.1 PREDICTED: TRINITY_DN7014_c0_g2_i LOW QUALITY PROTEIN: src XP_014362846.1 13037.EHJ64520 1.p1 substrate protein p85 [Papilio machaon] UniRef90_A0A2K3K0E9 Serine hydroxymethyltransferase TRINITY_DN115711_c0_g UniRef90_A0A2K3K0E9 mitochondrial-like (Fragment) n=2 3847.GLYMA08G37270.1 1_i1.p1 Tax=rosids TaxID=71275 RepID=A0A2K3K0E9_TRIPR tr|A0A0F6NDJ4|A0A0F6NDJ4_OS TRINITY_DN11574_c0_g1 tr|A0A0F6NDJ4|A0A0F6NDJ4_OS TNU Dscam (Fragment) 34740.HMEL007149-PA _i10.p3 TNU OS=Ostrinia nubilalis OX=29057 GN=dscam PE=4 SV=1 tr|A0A0F6NDJ4|A0A0F6NDJ4_OS TRINITY_DN11574_c0_g1 tr|A0A0F6NDJ4|A0A0F6NDJ4_OS TNU Dscam (Fragment) 34740.HMEL007149-PA _i15.p2 TNU OS=Ostrinia nubilalis OX=29057 GN=dscam PE=4 SV=1 tr|A0A0F6NCS4|A0A0F6NCS4_H TRINITY_DN11574_c0_g1 tr|A0A0F6NCS4|A0A0F6NCS4_H ELAM Dscam (Fragment) 34740.HMEL007149-PA _i2.p2 ELAM OS=Helicoverpa armigera OX=29058 GN=dscam PE=4 SV=1 tr|A0A0F6NDI4|A0A0F6NDI4_HE TRINITY_DN11574_c0_g1 tr|A0A0F6NDI4|A0A0F6NDI4_HE LAM Dscam (Fragment) 7091.BGIBMGA010363- _i7.p1 LAM OS=Helicoverpa armigera TA OX=29058 GN=dscam PE=4 SV=1 XP_026490772.1 TRINITY_DN5221_c0_g2_i XP_026490772.1 phosphatidylinositol 4-kinase alpha 13037.EHJ79076 2.p1 isoform X2 [Vanessa tameamea] XP_026500150.1 fibroblast growth TRINITY_DN18057_c0_g1 XP_026500150.1 factor 3 isoform X1 [Vanessa 13037.EHJ75405 _i5.p1 tameamea] XP_026688288.1 uncharacterized TRINITY_DN36274_c0_g1 XP_026688288.1 protein LOC113472698 [Diaphorina 34740.HMEL013671-PA _i1.p1 citri] TRINITY_DN75636_c0_g1 EDW29381.1 GL22940, partial EDW29381.1 13037.EHJ65580 _i1.p1 [Drosophila persimilis] TRINITY_DN3115_c0_g1_i XP_012552529.1 titin-like, partial XP_012552529.1 13037.EHJ65548 16.p2 [Bombyx mori] tr|A0A2H1WAK0|A0A2H1WAK0_ SPOFR SFRICE_008899 TRINITY_DN11184_c0_g1 tr|A0A2H1WAK0|A0A2H1WAK0_ OS=Spodoptera frugiperda 13037.EHJ71623 _i11.p4 SPOFR OX=7108 GN=SFRICE_008899 PE=4 SV=1 tr|A0A0F6NCP9|A0A0F6NCP9_H TRINITY_DN24209_c0_g2 tr|A0A0F6NCP9|A0A0F6NCP9_H ELAM Dscam (Fragment) 7165.AGAP010884-PA _i5.p1 ELAM OS=Helicoverpa armigera OX=29058 GN=dscam PE=4 SV=1 103

XP_014370744.1 PREDICTED: raf TRINITY_DN17059_c0_g2 homolog serine/threonine-protein XP_014370744.1 13037.EHJ72756 _i1.p2 kinase phl-like, partial [Papilio machaon] tr|A0A212F4X8|A0A212F4X8_DA NPL E3 ubiquitin-protein ligase TRINITY_DN11645_c0_g2 tr|A0A212F4X8|A0A212F4X8_DA MYLIP OS=Danaus plexippus 34740.HMEL017980-PA _i12.p1 NPL plexippus OX=278856 GN=KGM_201119 PE=4 SV=1 XP_032527910.1 OCIA domain- TRINITY_DN10255_c0_g1 XP_032527910.1 containing protein 1 isoform X1 34740.HMEL011027-PA _i4.p1 [Danaus plexippus plexippus] UniRef90_A0A1Y3ARZ6 DEK_C domain-containing protein TRINITY_DN17299_c0_g1 UniRef90_A0A1Y3ARZ6 (Fragment) n=1 Tax=Euroglyphus 13037.EHJ63863 _i16.p6 maynei TaxID=6958 RepID=A0A1Y3ARZ6_EURMA tr|A0A212EMA5|A0A212EMA5_D ANPL S-phase kinase-associated TRINITY_DN735_c0_g1_i1 tr|A0A212EMA5|A0A212EMA5_D protein OS=Danaus plexippus 13037.EHJ65429 .p1 ANPL plexippus OX=278856 GN=KGM_211966 PE=3 SV=1 tr|A0A0F6NDJ1|A0A0F6NDJ1_OS TRINITY_DN24209_c0_g2 tr|A0A0F6NDJ1|A0A0F6NDJ1_OS TNU Dscam (Fragment) 7213.XP_004520249.1 _i1.p5 TNU OS=Ostrinia nubilalis OX=29057 GN=dscam PE=4 SV=1 XP_026500178.1 another TRINITY_DN11710_c0_g1 7091.BGIBMGA010107- XP_026500178.1 transcription unit protein [Vanessa _i2.p1 TA tameamea] XP_026501329.1 condensin TRINITY_DN1423_c2_g1_i XP_026501329.1 complex subunit 3 [Vanessa 13037.EHJ79105 1.p1 tameamea] TRINITY_DN4256_c0_g1_i XP_026496410.1 endoribonuclease XP_026496410.1 13037.EHJ65725 18.p1 Dicer [Vanessa tameamea] TRINITY_DN1953_c0_g1_i XP_026497854.1 probable RNA XP_026497854.1 13037.EHJ78906 3.p1 helicase armi [Vanessa tameamea] tr|A0A0S7ET52|A0A0S7ET52_9T ELE DSCAM (Fragment) TRINITY_DN63204_c0_g1 tr|A0A0S7ET52|A0A0S7ET52_9T OS=Poeciliopsis prolifica 34740.HMEL002841-PA _i1.p1 ELE OX=188132 GN=DSCAM PE=4 SV=1 TRINITY_DN17001_c2_g1 34740.HMEL016482-PA _i1.p1 KAF2885102.1 hypothetical protein TRINITY_DN5691_c0_g1_i KAF2885102.1 ILUMI_21066, partial [Ignelater 34740.HMEL004653-PA 5.p2 luminosus] tr|A0A3S2LBY5|A0A3S2LBY5_C HISP Protein kinase domain- TRINITY_DN8606_c2_g1_i tr|A0A3S2LBY5|A0A3S2LBY5_C containing protein OS=Chilo 13037.EHJ79112 10.p1 HISP suppressalis OX=168631 GN=evm_005140 PE=4 SV=1 tr|A0A0F6NDL0|A0A0F6NDL0_S TRINITY_DN11574_c0_g1 tr|A0A0F6NDL0|A0A0F6NDL0_S POLT Dscam (Fragment) 34740.HMEL007149-PA _i10.p4 POLT OS=Spodoptera litura OX=69820 GN=dscam PE=4 SV=1 104

tr|A0S6A0|A0S6A0_SPOFR TRINITY_DN1338_c0_g1_i Prophenoloxidase subunit 1 tr|A0S6A0|A0S6A0_SPOFR 13037.EHJ78993 2.p1 OS=Spodoptera frugiperda OX=7108 PE=2 SV=1 tr|A0A212F0U5|A0A212F0U5_DA TRINITY_DN13455_c0_g1 tr|A0A212F0U5|A0A212F0U5_DA NPL C-type lectin 4 OS=Danaus 13037.EHJ70690 _i1.p2 NPL plexippus plexippus OX=278856 GN=KGM_214223 PE=4 SV=1 XP_026486456.1 apoptosis- TRINITY_DN12952_c0_g1 XP_026486456.1 resistant E3 ubiquitin protein ligase 13037.EHJ69238 _i34.p1 1 isoform X1 [Vanessa tameamea] tr|H9JDF6|H9JDF6_BOMMO C- TRINITY_DN3868_c0_g1_i type lectin domain-containing tr|H9JDF6|H9JDF6_BOMMO 34740.HMEL003322-PA 3.p1 protein OS=Bombyx mori OX=7091 PE=4 SV=1 TRINITY_DN7628_c0_g1_i XP_026496646.1 protein draper XP_026496646.1 13037.EHJ75645 2.p1 [Vanessa tameamea] tr|A0A1E1WSJ4|A0A1E1WSJ4_PE CGO Uncharacterized protein TRINITY_DN13908_c0_g2 tr|A0A1E1WSJ4|A0A1E1WSJ4_PE (Fragment) OS=Pectinophora 13037.EHJ73003 _i4.p1 CGO gossypiella OX=13191 GN=g.23 PE=4 SV=1 XP_022130726.1 peptidoglycan TRINITY_DN1760_c0_g1_i XP_022130726.1 recognition protein-like [Pieris 34740.HMEL006763-PA 2.p1 rapae] tr|A0A212F0W4|A0A212F0W4_D ANPL RNA helicase protein TRINITY_DN17697_c1_g1 tr|A0A212F0W4|A0A212F0W4_D OS=Danaus plexippus plexippus 13037.EHJ70710 _i3.p1 ANPL OX=278856 GN=KGM_213860 PE=4 SV=1 XP_026491540.1 tumor necrosis TRINITY_DN100005_c0_g XP_026491540.1 factor receptor superfamily member 34740.HMEL007101-PA 1_i1.p1 wengen [Vanessa tameamea] XP_026488554.1 CLIP domain- TRINITY_DN13075_c0_g1 XP_026488554.1 containing serine protease 2-like 34740.HMEL010779-PA _i2.p1 [Vanessa tameamea] KPJ13180.1 Ankyrin repeat TRINITY_DN3295_c1_g1_i KPJ13180.1 domain-containing protein 17 13037.EHJ73802 4.p1 [Papilio machaon] tr|A0A212FLT4|A0A212FLT4_DA TRINITY_DN7783_c0_g1_i tr|A0A212FLT4|A0A212FLT4_DA NPL Frizzled-2 OS=Danaus 13037.EHJ78851 13.p1 NPL plexippus plexippus OX=278856 GN=KGM_212368 PE=3 SV=1 tr|A0A212ESE0|A0A212ESE0_DA NPL Fibroblast growth factor TRINITY_DN3949_c0_g1_i tr|A0A212ESE0|A0A212ESE0_DA receptor OS=Danaus plexippus 13037.EHJ67396 1.p1 NPL plexippus OX=278856 GN=KGM_212041 PE=3 SV=1 XP_026494120.1 phenoloxidase- TRINITY_DN1193_c0_g1_i 7091.BGIBMGA014404- XP_026494120.1 activating factor 2-like isoform X1 1.p1 TA [Vanessa tameamea] XP_026494121.1 phenoloxidase- TRINITY_DN1193_c0_g1_i 7091.BGIBMGA014404- XP_026494121.1 activating factor 2-like isoform X2 4.p1 TA [Vanessa tameamea] 105

tr|A0A212F7U5|A0A212F7U5_DA NPL Cyclic AMP-dependent TRINITY_DN11996_c0_g1 tr|A0A212F7U5|A0A212F7U5_DA transcription factor ATF-3 13037.EHJ73325 _i1.p1 NPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_206613 PE=4 SV=1 tr|A0A212FLY5|A0A212FLY5_DA NPL C-type lectin domain- TRINITY_DN44_c0_g3_i1. tr|A0A212FLY5|A0A212FLY5_DA containing protein OS=Danaus 34740.HMEL008543-PA p1 NPL plexippus plexippus OX=278856 GN=KGM_215784 PE=4 SV=1 tr|A0A2H1VQI9|A0A2H1VQI9_SP OFR SFRICE_006705 TRINITY_DN11984_c1_g1 tr|A0A2H1VQI9|A0A2H1VQI9_SP OS=Spodoptera frugiperda 13037.EHJ71331 _i1.p1 OFR OX=7108 GN=SFRICE_006705 PE=4 SV=1 XP_026493979.1 tyrosine-protein TRINITY_DN18895_c0_g1 XP_026493979.1 kinase hopscotch [Vanessa 13037.EHJ65487 _i2.p1 tameamea] tr|S4P8Q7|S4P8Q7_9NEOP Sarah TRINITY_DN14521_c0_g1 tr|S4P8Q7|S4P8Q7_9NEOP OS=Pararge aegeria OX=116150 34740.HMEL010974-PA _i1.p1 PE=4 SV=1 TRINITY_DN5003_c0_g1_i XP_026488773.1 Krueppel-like XP_026488773.1 13037.EHJ67660 5.p2 factor 16 [Vanessa tameamea] TRINITY_DN2039_c0_g1_i XP_026499312.1 titin-like [Vanessa XP_026499312.1 13037.EHJ65548 4.p1 tameamea] tr|A0A212FFD5|A0A212FFD5_DA NPL Cell division cycle protein 23 TRINITY_DN12868_c0_g2 tr|A0A212FFD5|A0A212FFD5_DA OS=Danaus plexippus plexippus 13037.EHJ76144 _i1.p1 NPL OX=278856 GN=KGM_201340 PE=4 SV=1 tr|A0A2H1V4L3|A0A2H1V4L3_SP OFR SFRICE_013550 TRINITY_DN29133_c0_g1 tr|A0A2H1V4L3|A0A2H1V4L3_SP OS=Spodoptera frugiperda 34740.HMEL017581-PA _i2.p1 OFR OX=7108 GN=SFRICE_013550 PE=4 SV=1 tr|S4PDV1|S4PDV1_9NEOP S- TRINITY_DN12045_c2_g1 phase kinase-associated protein 2 tr|S4PDV1|S4PDV1_9NEOP 34740.HMEL010736-PA _i5.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 tr|S4PDV1|S4PDV1_9NEOP S- TRINITY_DN12045_c2_g1 phase kinase-associated protein 2 tr|S4PDV1|S4PDV1_9NEOP 34740.HMEL010736-PA _i6.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 tr|A0A2W1BTL8|A0A2W1BTL8_ HELAM PI3K/PI4K domain- TRINITY_DN1654_c0_g1_i tr|A0A2W1BTL8|A0A2W1BTL8_ containing protein OS=Helicoverpa 34740.HMEL003893-PA 33.p1 HELAM armigera OX=29058 GN=HaOG205269 PE=3 SV=1 tr|A0A194PI29|A0A194PI29_PAPX TRINITY_DN13467_c0_g1 tr|A0A194PI29|A0A194PI29_PAPX U Menin OS=Papilio xuthus 7091.BGIBMGA010655- _i1.p1 U OX=66420 GN=RR46_13929 PE=4 TA SV=1 UniRef90_R9W2R3 Dscam TRINITY_DN17986_c0_g1 (Fragment) n=2 Tax=Obtectomera UniRef90_R9W2R3 13037.EHJ73141 _i4.p2 TaxID=104431 RepID=R9W2R3_CHISP 106

XP_026494275.1 CLIP domain- TRINITY_DN1853_c0_g1_i XP_026494275.1 containing serine protease 2-like 13037.EHJ73834 12.p1 isoform X1 [Vanessa tameamea] XP_026484047.1 serine/threonine- TRINITY_DN1650_c0_g1_i XP_026484047.1 protein kinase MARK2-like isoform 13037.EHJ67415 3.p1 X6 [Vanessa tameamea] tr|A0A2H1VXV0|A0A2H1VXV0_ SPOFR SFRICE_035922 TRINITY_DN10553_c0_g2 tr|A0A2H1VXV0|A0A2H1VXV0_ OS=Spodoptera frugiperda 34740.HMEL016235-PA _i1.p1 SPOFR OX=7108 GN=SFRICE_035922 PE=4 SV=1 TRINITY_DN1212_c1_g1_i 13037.EHJ73834 1.p1 XP_022836604.1 uncharacterized TRINITY_DN4683_c1_g1_i XP_022836604.1 protein LOC111363943 [Spodoptera 13037.EHJ65343 4.p1 litura] PZC85920.1 hypothetical protein TRINITY_DN1791_c2_g1_i PZC85920.1 B5X24_HaOG214285 [Helicoverpa 34740.HMEL017750-PA 13.p3 armigera] tr|A0A212FLJ2|A0A212FLJ2_DA NPL MARVEL domain-containing TRINITY_DN17769_c0_g1 tr|A0A212FLJ2|A0A212FLJ2_DA protein (Fragment) OS=Danaus 13037.EHJ78735 _i5.p1 NPL plexippus plexippus OX=278856 GN=KGM_206087B PE=4 SV=1 tr|A0A194R4R6|A0A194R4R6_PA PMA Eukaryotic translation TRINITY_DN1233_c0_g1_i tr|A0A194R4R6|A0A194R4R6_PA initiation factor 4E-binding protein 13037.EHJ65071 10.p1 PMA 2 OS=Papilio machaon OX=76193 GN=RR48_10438 PE=4 SV=1 XP_026491162.1 transcriptional TRINITY_DN5118_c0_g2_i XP_026491162.1 enhancer factor TEF-1 isoform X1 13037.EHJ63836 6.p1 [Vanessa tameamea] TRINITY_DN18300_c0_g1 XP_026495604.1 protein spaetzle 3 XP_026495604.1 13037.EHJ76024 _i4.p1 [Vanessa tameamea] tr|A0A0N1PHJ0|A0A0N1PHJ0_PA TRINITY_DN402_c0_g1_i8 tr|A0A0N1PHJ0|A0A0N1PHJ0_PA PMA Trypsin CFT-1 OS=Papilio 34740.HMEL006941-PA .p1 PMA machaon OX=76193 GN=RR48_05552 PE=3 SV=1 tr|I4DNI1|I4DNI1_PAPXU TRINITY_DN1100_c0_g1_i Gamma-glutamylcyclotransferase 7091.BGIBMGA003537- tr|I4DNI1|I4DNI1_PAPXU 11.p2 OS=Papilio xuthus OX=66420 TA GN=RR46_12008 PE=2 SV=1 tr|A0A2H1WAK0|A0A2H1WAK0_ SPOFR SFRICE_008899 TRINITY_DN11184_c0_g1 tr|A0A2H1WAK0|A0A2H1WAK0_ OS=Spodoptera frugiperda 34740.HMEL013147-PA _i3.p4 SPOFR OX=7108 GN=SFRICE_008899 PE=4 SV=1 tr|H9JLG9|H9JLG9_BOMMO NR TRINITY_DN64800_c0_g1 LBD domain-containing protein tr|H9JLG9|H9JLG9_BOMMO 34740.HMEL011581-PA _i1.p1 OS=Bombyx mori OX=7091 PE=3 SV=1 tr|A0A212FG39|A0A212FG39_DA NPL Ubiquitin conjugating enzyme TRINITY_DN12452_c0_g1 tr|A0A212FG39|A0A212FG39_DA E2 OS=Danaus plexippus plexippus 13037.EHJ76453 _i1.p1 NPL OX=278856 GN=KGM_201405 PE=3 SV=1 107

tr|A0A194PT25|A0A194PT25_PAP XU Alpha-1,3-mannosyl- TRINITY_DN1393_c0_g1_i tr|A0A194PT25|A0A194PT25_PAP glycoprotein 2-beta-N- 34740.HMEL011176-PA 2.p1 XU acetylglucosaminyltransferase OS=Papilio xuthus OX=66420 GN=RR46_12497 PE=4 SV=1 XP_026488330.1 targeting protein TRINITY_DN12464_c0_g1 XP_026488330.1 for Xklp2 homolog [Vanessa 13037.EHJ77606 _i3.p1 tameamea] tr|A0A437AU47|A0A437AU47_C HISP LIM domain-containing TRINITY_DN1295_c3_g1_i tr|A0A437AU47|A0A437AU47_C protein OS=Chilo suppressalis 13037.EHJ74786 12.p1 HISP OX=168631 GN=evm_014033 PE=4 SV=1 tr|A0A194RQQ0|A0A194RQQ0_P APMA Baculoviral IAP repeat- TRINITY_DN1514_c0_g1_i tr|A0A194RQQ0|A0A194RQQ0_P 7091.BGIBMGA011523- containing protein 3 OS=Papilio 11.p1 APMA TA machaon OX=76193 GN=RR48_07411 PE=4 SV=1 XP_026486652.1 regulatory- TRINITY_DN5907_c0_g2_i XP_026486652.1 associated protein of mTOR 13037.EHJ76100 1.p1 [Vanessa tameamea] tr|H9JVB1|H9JVB1_BOMMO TRINITY_DN15912_c0_g1 RRM domain-containing protein tr|H9JVB1|H9JVB1_BOMMO 34740.HMEL008154-PA _i1.p1 OS=Bombyx mori OX=7091 PE=4 SV=1 tr|A0A2A4K9Y7|A0A2A4K9Y7_H ELVI Protein kinase domain- TRINITY_DN1114_c0_g2_i tr|A0A2A4K9Y7|A0A2A4K9Y7_H containing protein OS=Heliothis 13037.EHJ75547 13.p1 ELVI virescens OX=7102 GN=B5V51_37 PE=4 SV=1 XP_026489628.1 CLIP domain- TRINITY_DN12759_c0_g1 XP_026489628.1 containing serine protease 2 isoform 13037.EHJ78263 _i13.p1 X2 [Vanessa tameamea] TRINITY_DN2939_c1_g1_i 34740.HMEL003132-PA 6.p1 tr|A0A212ERZ7|A0A212ERZ7_DA NPL Tyrosine-protein kinase TRINITY_DN3163_c0_g1_i tr|A0A212ERZ7|A0A212ERZ7_DA OS=Danaus plexippus plexippus 13037.EHJ67223 13.p1 NPL OX=278856 GN=KGM_207312 PE=3 SV=1 tr|A0A212FCV3|A0A212FCV3_D ANPL Nuclear transcription factor TRINITY_DN5929_c0_g1_i tr|A0A212FCV3|A0A212FCV3_D Y subunit beta OS=Danaus 34740.HMEL005908-PA 18.p2 ANPL plexippus plexippus OX=278856 GN=KGM_210110 PE=4 SV=1 TRINITY_DN1070_c0_g2_i XP_026497729.1 gloverin-like 7091.BGIBMGA013863- XP_026497729.1 1.p1 [Vanessa tameamea] TA tr|S4PE12|S4PE12_9NEOP TP53- TRINITY_DN32065_c0_g1 tr|S4PE12|S4PE12_9NEOP regulating kinase OS=Pararge 34740.HMEL014501-PA _i1.p1 aegeria OX=116150 PE=4 SV=1 tr|A0A212F9C2|A0A212F9C2_DA TRINITY_DN4894_c0_g1_i tr|A0A212F9C2|A0A212F9C2_DA NPL Kibra isoform 1 OS=Danaus 13037.EHJ73876 11.p1 NPL plexippus plexippus OX=278856 GN=KGM_213788 PE=4 SV=1 108

tr|A0A5E4QA97|A0A5E4QA97_9 NEOP Mitogen-activated protein TRINITY_DN19972_c0_g1 tr|A0A5E4QA97|A0A5E4QA97_9 kinase OS=Leptidea sinapis 13037.EHJ76051 _i2.p1 NEOP OX=189913 GN=LSINAPIS_LOCUS6957 PE=4 SV=1 ACI32829.1 beta-1,3-glucan TRINITY_DN1140_c0_g1_i ACI32829.1 recognition protein 2, partial [Delias 34740.HMEL003270-PA 3.p1 nigrina] XP_023953530.1 uncharacterized TRINITY_DN73819_c0_g1 XP_023953530.1 protein LOC112057269, partial 34740.HMEL017773-PA _i1.p1 [Bicyclus anynana] tr|A0A2H1VN22|A0A2H1VN22_S POFR SFRICE_000235 TRINITY_DN11979_c0_g1 tr|A0A2H1VN22|A0A2H1VN22_S OS=Spodoptera frugiperda 13037.EHJ79029 _i6.p1 POFR OX=7108 GN=SFRICE_000235 PE=4 SV=1 tr|A0A0N0PAH7|A0A0N0PAH7_P APXU MAM domain-containing TRINITY_DN5296_c0_g1_i tr|A0A0N0PAH7|A0A0N0PAH7_P glycosylphosphatidylinositol anchor 34740.HMEL005258-PA 1.p1 APXU protein 1 OS=Papilio xuthus OX=66420 GN=RR46_01259 PE=4 SV=1 XP_026498635.1 uncharacterized TRINITY_DN130167_c0_g XP_026498635.1 protein LOC113402560 [Vanessa 13037.EHJ77882 1_i1.p1 tameamea] tr|A0A194PS51|A0A194PS51_PAP XU Reelin domain-containing TRINITY_DN11432_c0_g1 tr|A0A194PS51|A0A194PS51_PAP protein OS=Papilio xuthus 34740.HMEL011384-PA _i1.p2 XU OX=66420 GN=RR46_13120 PE=4 SV=1 tr|A0A2H1WCR1|A0A2H1WCR1_ SPOFR SFRICE_006653 TRINITY_DN3443_c0_g2_i tr|A0A2H1WCR1|A0A2H1WCR1_ OS=Spodoptera frugiperda 13037.EHJ75874 4.p1 SPOFR OX=7108 GN=SFRICE_006653 PE=4 SV=1 tr|A0A194RM97|A0A194RM97_P APMA Serine/threonine-protein TRINITY_DN6686_c0_g1_i tr|A0A194RM97|A0A194RM97_P kinase TOR OS=Papilio machaon 13037.EHJ65030 3.p1 APMA OX=76193 GN=RR48_10596 PE=3 SV=1 tr|S4PB13|S4PB13_9NEOP Caudal TRINITY_DN17036_c0_g1 tr|S4PB13|S4PB13_9NEOP OS=Pararge aegeria OX=116150 34740.HMEL016680-PA _i5.p1 PE=4 SV=1 TRINITY_DN4404_c0_g1_i 7091.BGIBMGA000320-

24.p3 TA TRINITY_DN1983_c0_g1_i XP_026496542.1 cactin [Vanessa XP_026496542.1 34740.HMEL006752-PA 2.p1 tameamea] tr|C0PUJ8|C0PUJ8_SALSA Probable ATP-dependent RNA TRINITY_DN971_c0_g1_i1 tr|C0PUJ8|C0PUJ8_SALSA helicase DDX5 (Fragment) 34740.HMEL013855-PA .p1 OS=Salmo salar OX=8030 GN=DDX5 PE=2 SV=1 tr|A0A2H1VBY5|A0A2H1VBY5_S POFR SFRICE_017792 TRINITY_DN3037_c0_g1_i tr|A0A2H1VBY5|A0A2H1VBY5_S OS=Spodoptera frugiperda 34740.HMEL007265-PA 1.p1 POFR OX=7108 GN=SFRICE_017792 PE=4 SV=1 109

tr|A0A2A4IYI9|A0A2A4IYI9_HEL VI Small nuclear ribonucleoprotein TRINITY_DN129851_c0_g tr|A0A2A4IYI9|A0A2A4IYI9_HEL Sm D3 OS=Heliothis virescens 34740.HMEL017608-PA 1_i1.p1 VI OX=7102 GN=B5V51_10061 PE=3 SV=1 XP_026490761.1 adenosine TRINITY_DN567_c0_g1_i2 XP_026490761.1 deaminase 2-like [Vanessa 34740.HMEL004268-PA 5.p1 tameamea] KAE9465506.1 hypothetical protein TRINITY_DN135268_c0_g 29760.VIT_01s0011g0190 KAE9465506.1 C3L33_02589, partial 1_i1.p1 0.t01 [Rhododendron williamsianum] tr|C9DTM4|C9DTM4_BOMMO TRINITY_DN1298_c0_g1_i 7091.BGIBMGA012467- tr|C9DTM4|C9DTM4_BOMMO CAP isoform A OS=Bombyx mori 26.p2 TA OX=7091 PE=2 SV=1 tr|A0A2H1V610|A0A2H1V610_SP OFR SFRICE_033506 TRINITY_DN116037_c0_g tr|A0A2H1V610|A0A2H1V610_SP 7091.BGIBMGA001303- OS=Spodoptera frugiperda 1_i1.p1 OFR TA OX=7108 GN=SFRICE_033506 PE=4 SV=1 XP_026494921.1 transcription TRINITY_DN12438_c0_g1 XP_026494921.1 initiation factor TFIID subunit 2 34740.HMEL007759-PA _i2.p1 [Vanessa tameamea] tr|V9L4B8|V9L4B8_CALMI MAP/microtubule affinity- TRINITY_DN18_c2_g3_i2. tr|V9L4B8|V9L4B8_CALMI regulating kinase 2 (Fragment) 34740.HMEL011289-PA p1 OS=Callorhinchus milii OX=7868 PE=2 SV=1 UniRef90_A0A329PFV3 Lysozyme TRINITY_DN131036_c0_g 68219.JNXI01000031_gen UniRef90_A0A329PFV3 n=8 Tax=Bacteria TaxID=2 1_i1.p1 e5514 RepID=A0A329PFV3_9LACT TRINITY_DN3589_c1_g1_i 13037.EHJ76429 19.p1 UniRef90_UPI000C1CE6A2 8- hydroxygeraniol dehydrogenase- TRINITY_DN131832_c0_g 4113.PGSC0003DMT400 UniRef90_UPI000C1CE6A2 like n=1 Tax=Olea europaea var. 1_i1.p1 041566 sylvestris TaxID=158386 RepID=UPI000C1CE6A2 tr|A0A212EZI1|A0A212EZI1_DA TRINITY_DN154_c1_g1_i1 tr|A0A212EZI1|A0A212EZI1_DA NPL Fas OS=Danaus plexippus 13037.EHJ70237 2.p1 NPL plexippus OX=278856 GN=KGM_210418 PE=4 SV=1 tr|A0A2H1VCR1|A0A2H1VCR1_S POFR SFRICE_002609 TRINITY_DN11466_c0_g1 tr|A0A2H1VCR1|A0A2H1VCR1_S OS=Spodoptera frugiperda 13037.EHJ66311 _i1.p1 POFR OX=7108 GN=SFRICE_002609 PE=4 SV=1 XP_014361459.1 PREDICTED: TRINITY_DN133193_c0_g LOW QUALITY PROTEIN: XP_014361459.1 34740.HMEL016530-PA 1_i1.p1 acetylcholine receptor subunit beta- like 2, partial [Papilio machaon] TRINITY_DN85452_c0_g1 XP_028042269.1 protein sickie- XP_028042269.1 34740.HMEL015696-PA _i1.p1 like, partial [Bombyx mandarina] XP_026501465.1 myeloid TRINITY_DN1300_c0_g1_i XP_026501465.1 differentiation primary response 13037.EHJ73995 1.p1 protein MyD88 [Vanessa tameamea] XP_026490933.1 cytokine receptor- TRINITY_DN1621_c0_g1_i XP_026490933.1 like isoform X1 [Vanessa 34740.HMEL000037-PA 1.p1 tameamea] 110

TRINITY_DN1979_c0_g2_i 13037.EHJ70551 1.p1 tr|A0A0N1IIT2|A0A0N1IIT2_PAP MA Dual specificity protein TRINITY_DN19801_c0_g1 tr|A0A0N1IIT2|A0A0N1IIT2_PAP phosphatase 12 OS=Papilio 34740.HMEL010016-PA _i1.p1 MA machaon OX=76193 GN=RR48_04889 PE=4 SV=1 XP_014370744.1 PREDICTED: raf TRINITY_DN17059_c0_g1 homolog serine/threonine-protein XP_014370744.1 13037.EHJ72756 _i11.p4 kinase phl-like, partial [Papilio machaon] XP_026493535.1 ubiquitin-like TRINITY_DN1992_c0_g1_i XP_026493535.1 modifier-activating enzyme ATG7 34740.HMEL004052-PA 1.p1 [Vanessa tameamea] tr|S4PGC3|S4PGC3_9NEOP Zinc TRINITY_DN4771_c0_g2_i finger protein on ecdysone puffs tr|S4PGC3|S4PGC3_9NEOP 34740.HMEL013337-PA 11.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 TRINITY_DN3431_c1_g1_i XP_026485857.1 afadin isoform X1 XP_026485857.1 34740.HMEL018030-PA 1.p1 [Vanessa tameamea] tr|A0A212FF41|A0A212FF41_DA NPL Mitogen-activated protein TRINITY_DN19972_c0_g1 tr|A0A212FF41|A0A212FF41_DA kinase OS=Danaus plexippus 13037.EHJ76051 _i10.p1 NPL plexippus OX=278856 GN=KGM_215664 PE=4 SV=1 TRINITY_DN1983_c0_g1_i XP_026496542.1 cactin [Vanessa XP_026496542.1 34740.HMEL006752-PA 5.p1 tameamea] tr|A0A3B4BQ50|A0A3B4BQ50_P TRINITY_DN40199_c0_g2 tr|A0A3B4BQ50|A0A3B4BQ50_P YGNA Cyclin-dependent kinase 2 13037.EHJ65237 _i4.p1 YGNA OS=Pygocentrus nattereri OX=42514 PE=3 SV=1 UniRef90_A0A0S7G198 RAE1L TRINITY_DN5027_c0_g1_i (Fragment) n=1 Tax=Poeciliopsis UniRef90_A0A0S7G198 34740.HMEL008585-PA 3.p2 prolifica TaxID=188132 RepID=A0A0S7G198_9TELE TRINITY_DN2997_c0_g1_i GBP13799.1 Cyclin-dependent-like GBP13799.1 34740.HMEL007938-PA 11.p2 kinase 5 [Eumeta japonica] XP_022118237.1 longitudinals TRINITY_DN1351_c0_g1_i XP_022118237.1 lacking protein, isoforms F/I/K/T- 13037.EHJ78854 2.p2 like isoform X3 [Pieris rapae] tr|A0A2W1BL66|A0A2W1BL66_H ELAM Uncharacterized protein TRINITY_DN20053_c0_g1 tr|A0A2W1BL66|A0A2W1BL66_H OS=Helicoverpa armigera 34740.HMEL018030-PA _i10.p1 ELAM OX=29058 GN=HaOG209491 PE=4 SV=1 TRINITY_DN20113_c0_g2 34740.HMEL008582-PA _i6.p1 XP_023948144.1 transcription TRINITY_DN40539_c0_g1 XP_023948144.1 factor collier isoform X5 [Bicyclus 13037.EHJ63297 _i1.p1 anynana] tr|A0A212EHH7|A0A212EHH7_D ANPL Zinc finger protein NANOS- TRINITY_DN20145_c0_g1 tr|A0A212EHH7|A0A212EHH7_D P OS=Danaus plexippus plexippus 13037.EHJ63417 _i1.p1 ANPL OX=278856 GN=KGM_205136 PE=3 SV=1 111

tr|A0A0F6NDA9|A0A0F6NDA9_O TRINITY_DN11574_c0_g1 tr|A0A0F6NDA9|A0A0F6NDA9_O STNU Dscam (Fragment) 34740.HMEL007149-PA _i10.p2 STNU OS=Ostrinia nubilalis OX=29057 GN=dscam PE=4 SV=1 tr|A0A2Z4N450|A0A2Z4N450_9N TRINITY_DN20163_c0_g1 tr|A0A2Z4N450|A0A2Z4N450_9N EOP Superoxide dismutase 13037.EHJ68139 _i3.p1 EOP OS=Heortia vitessoides OX=1557813 PE=2 SV=1 TRINITY_DN33844_c0_g1 XP_026497654.1 caspase-3-like XP_026497654.1 34740.HMEL007958-PA _i4.p1 [Vanessa tameamea] tr|A0A3S5XAC5|A0A3S5XAC5_A TRINITY_DN10653_c0_g1 tr|A0A3S5XAC5|A0A3S5XAC5_A NTPE Serpin-1 (Fragment) 13037.EHJ75278 _i11.p1 NTPE OS=Antheraea pernyi OX=7119 PE=2 SV=1 tr|A0A212ENJ9|A0A212ENJ9_DA NPL Transforming protein p54/c- TRINITY_DN3907_c0_g1_i tr|A0A212ENJ9|A0A212ENJ9_DA ets-1 OS=Danaus plexippus 34740.HMEL004734-PA 1.p1 NPL plexippus OX=278856 GN=KGM_202302 PE=3 SV=1 XP_026495419.1 E3 SUMO- TRINITY_DN3018_c0_g2_i XP_026495419.1 protein ligase PIAS3 isoform X2 34740.HMEL005634-PA 9.p1 [Vanessa tameamea] tr|A0A2A4K9Y9|A0A2A4K9Y9_H ELVI HMG box domain-containing TRINITY_DN2519_c0_g1_i tr|A0A2A4K9Y9|A0A2A4K9Y9_H protein OS=Heliothis virescens 13037.EHJ72465 19.p1 ELVI OX=7102 GN=B5V51_4064 PE=4 SV=1 sp|Q8SWR2|BICRD_DROME Bicaudal D-related protein homolog TRINITY_DN2035_c4_g1_i 7091.BGIBMGA013228- sp|Q8SWR2|BICRD_DROME OS=Drosophila melanogaster 11.p1 TA OX=7227 GN=CG17365 PE=2 SV=1 TRINITY_DN3431_c0_g1_i XP_026485861.1 afadin isoform X3 XP_026485861.1 34740.HMEL018030-PA 12.p1 [Vanessa tameamea] XP_026486239.1 poly [ADP- TRINITY_DN3675_c0_g1_i XP_026486239.1 ribose] polymerase [Vanessa 13037.EHJ70647 2.p1 tameamea] tr|A0A2H1VI86|A0A2H1VI86_SP OFR SFRICE_021037 TRINITY_DN3265_c0_g1_i tr|A0A2H1VI86|A0A2H1VI86_SP OS=Spodoptera frugiperda 34740.HMEL003402-PA 4.p1 OFR OX=7108 GN=SFRICE_021037 PE=4 SV=1 tr|A0A194Q8L7|A0A194Q8L7_PA TRINITY_DN2046_c1_g1_i tr|A0A194Q8L7|A0A194Q8L7_PA PXU Insulin receptor substrate 1 13037.EHJ66806 14.p1 PXU OS=Papilio xuthus OX=66420 GN=RR46_04724 PE=4 SV=1 tr|Q95W07|Q95W07_DROYA TRINITY_DN24860_c0_g1 DSCAM splice variant 4.7 tr|Q95W07|Q95W07_DROYA 7227.FBpp0110354 _i3.p1 (Fragment) OS=Drosophila yakuba OX=7245 PE=4 SV=1 XP_026489153.1 zinc finger TRINITY_DN2056_c0_g1_i XP_026489153.1 protein 1 isoform X2 [Vanessa 13037.EHJ68998 10.p1 tameamea] tr|A0A2H1VYV5|A0A2H1VYV5_ SPOFR SFRICE_031476 TRINITY_DN12403_c0_g1 tr|A0A2H1VYV5|A0A2H1VYV5_ (Fragment) OS=Spodoptera 13037.EHJ73373 _i3.p1 SPOFR frugiperda OX=7108 GN=SFRICE_031476 PE=4 SV=1 112

tr|S4PYB6|S4PYB6_9NEOP Ras- TRINITY_DN20699_c1_g1 related GTP-binding protein tr|S4PYB6|S4PYB6_9NEOP 34740.HMEL006696-PA _i2.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 XP_026487048.1 bifunctional TRINITY_DN5243_c0_g1_i arginine demethylase and lysyl- XP_026487048.1 34740.HMEL009014-PA 1.p1 hydroxylase PSR [Vanessa tameamea] tr|A0A212EHT2|A0A212EHT2_D ANPL alpha-1,2-Mannosidase TRINITY_DN2074_c0_g1_i tr|A0A212EHT2|A0A212EHT2_D OS=Danaus plexippus plexippus 13037.EHJ63518 11.p1 ANPL OX=278856 GN=KGM_208914 PE=3 SV=1 tr|S4PBQ2|S4PBQ2_9NEOP Rac1 TRINITY_DN3012_c2_g1_i tr|S4PBQ2|S4PBQ2_9NEOP OS=Pararge aegeria OX=116150 34740.HMEL007742-PA 1.p1 PE=4 SV=1 TRINITY_DN20884_c0_g1 7091.BGIBMGA000420-

_i6.p1 TA tr|A0A3S2NM10|A0A3S2NM10_C HISP Protein kinase domain- TRINITY_DN20884_c0_g2 tr|A0A3S2NM10|A0A3S2NM10_C containing protein OS=Chilo 13037.EHJ71993 _i2.p1 HISP suppressalis OX=168631 GN=evm_000856 PE=3 SV=1 TRINITY_DN23252_c0_g1 34740.HMEL009973-PA _i44.p4 tr|S4PB42|S4PB42_9NEOP TRINITY_DN2106_c0_g1_i Proliferating cell nuclear antigen tr|S4PB42|S4PB42_9NEOP 13037.EHJ67261 2.p1 OS=Pararge aegeria OX=116150 PE=3 SV=1 TRINITY_DN21074_c0_g2 XP_026495858.1 lysozyme-like XP_026495858.1 13037.EHJ72779 _i3.p1 [Vanessa tameamea] XP_026492750.1 uncharacterized TRINITY_DN32143_c0_g1 XP_026492750.1 protein LOC113398306 [Vanessa 13037.EHJ70909 _i2.p1 tameamea] tr|A0A2A4J8P8|A0A2A4J8P8_HEL TRINITY_DN13653_c0_g1 tr|A0A2A4J8P8|A0A2A4J8P8_HEL VI SMB domain-containing protein 13037.EHJ79208 _i3.p1 VI OS=Heliothis virescens OX=7102 GN=B5V51_5444 PE=4 SV=1 TRINITY_DN11859_c0_g1 XP_026484692.1 protein expanded XP_026484692.1 34740.HMEL008273-PA _i2.p1 [Vanessa tameamea] TRINITY_DN2134_c0_g2_i RVE52954.1 hypothetical protein RVE52954.1 13037.EHJ64422 2.p3 evm_002431 [Chilo suppressalis] tr|A0A212ENW7|A0A212ENW7_ DANPL Tyrosine-protein kinase TRINITY_DN7771_c0_g1_i tr|A0A212ENW7|A0A212ENW7_ OS=Danaus plexippus plexippus 13037.EHJ66028 4.p1 DANPL OX=278856 GN=KGM_207465 PE=3 SV=1 TRINITY_DN6938_c0_g1_i XP_023952685.1 C-type lectin XP_023952685.1 13037.EHJ68272 1.p1 37Db-like [Bicyclus anynana] XP_026484751.1 GATA-binding TRINITY_DN2500_c0_g3_i XP_026484751.1 factor 5-A-like isoform X6 [Vanessa 34740.HMEL007455-PA 2.p1 tameamea] tr|A0A212EJV9|A0A212EJV9_DA NPL Transcriptional regulator TRINITY_DN4391_c0_g1_i tr|A0A212EJV9|A0A212EJV9_DA ATRX OS=Danaus plexippus 13037.EHJ64400 4.p1 NPL plexippus OX=278856 GN=KGM_202629 PE=4 SV=1 113

XP_026501273.1 histone-lysine N- TRINITY_DN4044_c0_g1_i XP_026501273.1 methyltransferase SETD1 isoform 13037.EHJ79119 3.p1 X2 [Vanessa tameamea] TRINITY_DN37971_c0_g1 34740.HMEL005137-PA _i1.p1 TRINITY_DN2152_c0_g1_i PCG67848.1 hypothetical protein PCG67848.1 13037.EHJ65487 2.p1 B5V51_5899 [Heliothis virescens] tr|A0A212F5K0|A0A212F5K0_DA NPL Mediator of RNA polymerase TRINITY_DN215_c0_g1_i6 tr|A0A212F5K0|A0A212F5K0_DA 2 transcription subunit 25 34740.HMEL004110-PA 9.p1 NPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_203515 PE=4 SV=1 XP_032520898.1 ATP-dependent TRINITY_DN2165_c0_g1_i XP_032520898.1 RNA helicase dbp2-like isoform X2 34740.HMEL013855-PA 6.p1 [Danaus plexippus plexippus] tr|A0A2H1VTY0|A0A2H1VTY0_S POFR Deoxyhypusine hydroxylase TRINITY_DN218_c2_g1_i1 tr|A0A2H1VTY0|A0A2H1VTY0_S OS=Spodoptera frugiperda 13037.EHJ63855 .p1 POFR OX=7108 GN=SFRICE_012299 PE=3 SV=1 XP_026492364.1 serine protease TRINITY_DN21_c1_g1_i1. XP_026492364.1 persephone-like isoform X1 34740.HMEL011930-PA p1 [Vanessa tameamea] XP_026487701.1 chromodomain- TRINITY_DN2202_c0_g1_i XP_026487701.1 helicase-DNA-binding protein 7 34740.HMEL006822-PA 1.p1 isoform X1 [Vanessa tameamea] XP_026485623.1 ankyrin repeat TRINITY_DN1288_c1_g1_i XP_026485623.1 domain-containing protein 17 13037.EHJ73802 33.p1 [Vanessa tameamea] tr|A0A212FDI1|A0A212FDI1_DA NPL WD repeat domain TRINITY_DN2211_c2_g2_i tr|A0A212FDI1|A0A212FDI1_DA phosphoinositide-interacting protein 34740.HMEL009849-PA 2.p1 NPL 3 OS=Danaus plexippus plexippus OX=278856 GN=KGM_213443 PE=4 SV=1 XP_026501524.1 dual specificity TRINITY_DN25700_c0_g1 7091.BGIBMGA002807- XP_026501524.1 protein phosphatase 19 [Vanessa _i3.p2 TA tameamea] TRINITY_DN1395_c0_g1_i XP_026497727.1 lysozyme-like XP_026497727.1 34740.HMEL002874-PA 2.p1 isoform X2 [Vanessa tameamea] TRINITY_DN22201_c0_g2 XP_026491582.1 apoptosis XP_026491582.1 34740.HMEL016681-PA _i1.p1 inhibitor 5 [Vanessa tameamea] XP_026501135.1 apoptosis TRINITY_DN22269_c0_g2 XP_026501135.1 regulatory protein Siva-like isoform 34740.HMEL008655-PA _i1.p1 X2 [Vanessa tameamea] tr|A0A212EKQ6|A0A212EKQ6_D ANPL Structural maintenance of TRINITY_DN2234_c0_g1_i tr|A0A212EKQ6|A0A212EKQ6_D chromosomes protein OS=Danaus 13037.EHJ64697 1.p1 ANPL plexippus plexippus OX=278856 GN=KGM_205298 PE=3 SV=1 TRINITY_DN2519_c0_g1_i 13037.EHJ72465 47.p4 TRINITY_DN1464_c1_g1_i XP_026496476.1 optineurin XP_026496476.1 13037.EHJ68254 3.p1 isoform X1 [Vanessa tameamea] 114

XP_026501764.1 ALK tyrosine TRINITY_DN22558_c0_g1 XP_026501764.1 kinase receptor isoform X1 13037.EHJ73692 _i17.p1 [Vanessa tameamea] XP_026488105.1 WD repeat TRINITY_DN3993_c0_g1_i domain phosphoinositide- XP_026488105.1 34740.HMEL002443-PA 11.p1 interacting protein 2 isoform X3 [Vanessa tameamea] TRINITY_DN2261_c0_g2_i XP_026737876.1 cullin-3 isoform XP_026737876.1 34740.HMEL011639-PA 8.p1 X1 [Trichoplusia ni] tr|S4P7C5|S4P7C5_9NEOP TRINITY_DN22694_c0_g1 tr|S4P7C5|S4P7C5_9NEOP Roughened OS=Pararge aegeria 34740.HMEL006432-PA _i1.p1 OX=116150 PE=4 SV=1 tr|A0A0N0P9P6|A0A0N0P9P6_PA PXU Ankyrin repeat and SOCS box TRINITY_DN22828_c0_g1 tr|A0A0N0P9P6|A0A0N0P9P6_PA protein 17 OS=Papilio xuthus 34740.HMEL010032-PA _i4.p2 PXU OX=66420 GN=RR46_01473 PE=4 SV=1 XP_026483372.1 mitogen-activated TRINITY_DN4681_c0_g2_i XP_026483372.1 protein kinase-binding protein 1 34740.HMEL015734-PA 1.p1 isoform X5 [Vanessa tameamea] XP_026484801.1 anaphase- TRINITY_DN4361_c0_g1_i XP_026484801.1 promoting complex subunit 15-like 34740.HMEL006546-PA 1.p4 [Vanessa tameamea] XP_026498695.1 enhancer of TRINITY_DN6485_c0_g1_i XP_026498695.1 mRNA-decapping protein 4-like, 34740.HMEL012843-PA 4.p1 partial [Vanessa tameamea] XP_026492644.1 glutathione S- TRINITY_DN147683_c0_g XP_026492644.1 transferase E14-like [Vanessa 13037.EHJ71343 1_i1.p1 tameamea] TRINITY_DN2317_c0_g1_i XP_026489613.1 protein pellino XP_026489613.1 7070.TC009672-PA 11.p1 [Vanessa tameamea] XP_026488950.1 nuclear pore TRINITY_DN3276_c0_g1_i XP_026488950.1 complex protein Nup88 [Vanessa 13037.EHJ71248 1.p1 tameamea] tr|A0A3S2P011|A0A3S2P011_CHI TRINITY_DN6663_c0_g1_i tr|A0A3S2P011|A0A3S2P011_CHI SP Uncharacterized protein 13037.EHJ76100 14.p1 SP OS=Chilo suppressalis OX=168631 GN=evm_006471 PE=4 SV=1 tr|A0A212ETV7|A0A212ETV7_D ANPL Cysteine protease TRINITY_DN13275_c0_g1 tr|A0A212ETV7|A0A212ETV7_D OS=Danaus plexippus plexippus 34740.HMEL007647-PA _i1.p1 ANPL OX=278856 GN=KGM_202319 PE=3 SV=1 TRINITY_DN2342_c0_g1_i XP_026501604.1 paxillin isoform XP_026501604.1 7165.AGAP008532-PA 13.p1 X3 [Vanessa tameamea] TRINITY_DN2342_c0_g1_i XP_026501607.1 paxillin isoform XP_026501607.1 13037.EHJ66387 3.p1 X6 [Vanessa tameamea] TRINITY_DN81238_c0_g1 XP_030039894.1 protein jagged-2- XP_030039894.1 34740.HMEL008650-PA _i1.p1 like, partial [Manduca sexta] tr|A0A2P1DM87|A0A2P1DM87_S TRINITY_DN23547_c0_g2 tr|A0A2P1DM87|A0A2P1DM87_S POFR Pasha-PA OS=Spodoptera 13037.EHJ71192 _i2.p1 POFR frugiperda OX=7108 PE=2 SV=1 tr|A0A212FMG7|A0A212FMG7_D ANPL Receptor protein-tyrosine TRINITY_DN4741_c0_g2_i tr|A0A212FMG7|A0A212FMG7_D kinase OS=Danaus plexippus 13037.EHJ79031 1.p1 ANPL plexippus OX=278856 GN=KGM_206801 PE=3 SV=1 115

tr|A0A194QEL5|A0A194QEL5_PA TRINITY_DN2734_c0_g1_i tr|A0A194QEL5|A0A194QEL5_PA PXU Autophagy-related protein 13 34740.HMEL006890-PA 1.p1 PXU OS=Papilio xuthus OX=66420 GN=RR46_01874 PE=3 SV=1 tr|A0A212F5E7|A0A212F5E7_DA TRINITY_DN1664_c0_g2_i tr|A0A212F5E7|A0A212F5E7_DA NPL Crumbs OS=Danaus plexippus 13037.EHJ72433 1.p1 NPL plexippus OX=278856 GN=KGM_215296 PE=4 SV=1 tr|A0A2A4K5R1|A0A2A4K5R1_H ELVI Non-specific serine/threonine TRINITY_DN1650_c0_g1_i tr|A0A2A4K5R1|A0A2A4K5R1_H protein kinase OS=Heliothis 13037.EHJ67415 1.p1 ELVI virescens OX=7102 GN=B5V51_2331 PE=4 SV=1 TRINITY_DN2903_c0_g1_i XP_026488714.1 transmembrane XP_026488714.1 34740.HMEL003175-PA 1.p1 protein 183 [Vanessa tameamea] tr|A0A212FL92|A0A212FL92_DA TRINITY_DN6665_c0_g1_i tr|A0A212FL92|A0A212FL92_DA NPL Decapentaplegic OS=Danaus 13037.EHJ78539 1.p1 NPL plexippus plexippus OX=278856 GN=KGM_214072 PE=3 SV=1 tr|A0A0F6NCS9|A0A0F6NCS9_H TRINITY_DN24209_c0_g1 tr|A0A0F6NCS9|A0A0F6NCS9_H ELAM Dscam (Fragment) 34740.HMEL007149-PA _i3.p1 ELAM OS=Helicoverpa armigera OX=29058 GN=dscam PE=4 SV=1 XP_026490920.1 DNA TRINITY_DN1227_c1_g1_i XP_026490920.1 fragmentation factor subunit beta 34740.HMEL000043-PA 1.p1 isoform X1 [Vanessa tameamea] XP_026493596.1 paired box protein TRINITY_DN24063_c0_g1 XP_026493596.1 Pax-5 isoform X3 [Vanessa 34740.HMEL003749-PA _i2.p1 tameamea] tr|E9JEG8|E9JEG8_BOMMO TRINITY_DN2652_c0_g1_i tr|E9JEG8|E9JEG8_BOMMO ASK1 OS=Bombyx mori OX=7091 34740.HMEL006581-PA 1.p1 GN=100529210 PE=2 SV=1 XP_017149207.1 PREDICTED: TRINITY_DN2418_c0_g1_i serrate RNA effector molecule XP_017149207.1 13037.EHJ77624 2.p2 homolog isoform X3 [Drosophila miranda] tr|A0A5E4R4K7|A0A5E4R4K7_9N EOP PDGF_2 domain-containing TRINITY_DN241_c15_g1_i tr|A0A5E4R4K7|A0A5E4R4K7_9N protein OS=Leptidea sinapis 13037.EHJ68583 1.p1 EOP OX=189913 GN=LSINAPIS_LOCUS14863 PE=3 SV=1 TRINITY_DN24209_c0_g1 AII71600.1 Dscam, partial AII71600.1 34740.HMEL007149-PA _i4.p1 [Helicoverpa armigera] tr|A0A0F6NCU0|A0A0F6NCU0_O TRINITY_DN24209_c0_g2 tr|A0A0F6NCU0|A0A0F6NCU0_O STNU Dscam (Fragment) 7165.AGAP010884-PA _i14.p1 STNU OS=Ostrinia nubilalis OX=29057 GN=dscam PE=4 SV=1 tr|A0A0F6NDJ1|A0A0F6NDJ1_OS TRINITY_DN24209_c0_g2 tr|A0A0F6NDJ1|A0A0F6NDJ1_OS TNU Dscam (Fragment) 7091.BGIBMGA010363- _i2.p1 TNU OS=Ostrinia nubilalis OX=29057 TA GN=dscam PE=4 SV=1 tr|A0A0F6NDA7|A0A0F6NDA7_O TRINITY_DN24209_c0_g2 tr|A0A0F6NDA7|A0A0F6NDA7_O STNU Dscam (Fragment) 7070.TC012539-PA _i3.p1 STNU OS=Ostrinia nubilalis OX=29057 GN=dscam PE=4 SV=1 116

tr|A0A194QEZ3|A0A194QEZ3_PA PXU SUMO-activating enzyme TRINITY_DN2425_c0_g1_i tr|A0A194QEZ3|A0A194QEZ3_PA 7091.BGIBMGA002755- subunit 1 OS=Papilio xuthus 1.p1 PXU TA OX=66420 GN=RR46_07806 PE=4 SV=1 tr|A0A194PTD0|A0A194PTD0_PA TRINITY_DN4119_c0_g2_i tr|A0A194PTD0|A0A194PTD0_PA PXU CLIP-associating protein 13037.EHJ66282 14.p1 PXU OS=Papilio xuthus OX=66420 GN=RR46_12706 PE=4 SV=1 XP_026487083.1 cytoplasmic TRINITY_DN3759_c0_g1_i XP_026487083.1 FMR1-interacting protein [Vanessa 13037.EHJ75759 6.p1 tameamea] tr|S4PCP7|S4PCP7_9NEOP TRINITY_DN8661_c0_g1_i Glucosidase ii beta subunit tr|S4PCP7|S4PCP7_9NEOP 34740.HMEL007862-PA 1.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 XP_026500891.1 serine/threonine- TRINITY_DN14_c2_g2_i1. XP_026500891.1 protein kinase OSR1-like [Vanessa 13037.EHJ78782 p1 tameamea] XP_032526013.1 dynactin subunit TRINITY_DN43_c9_g1_i17 XP_032526013.1 1 isoform X8 [Danaus plexippus 13037.EHJ74738 .p1 plexippus] XP_026488218.1 rho GTPase- TRINITY_DN59823_c0_g1 XP_026488218.1 activating protein 44-like [Vanessa 13037.EHJ76164 _i5.p1 tameamea] tr|A0A212F942|A0A212F942_DA NPL WD repeat domain TRINITY_DN4329_c0_g1_i tr|A0A212F942|A0A212F942_DA phosphoinositide-interacting protein 34740.HMEL002443-PA 12.p1 NPL 2 OS=Danaus plexippus plexippus OX=278856 GN=KGM_201129 PE=4 SV=1 tr|I3V9Q7|I3V9Q7_VANCA IMD- TRINITY_DN24496_c0_g2 tr|I3V9Q7|I3V9Q7_VANCA like protein OS=Vanessa cardui 13037.EHJ71355 _i1.p1 OX=171605 GN=imd PE=2 SV=1 XP_026485871.1 dual specificity TRINITY_DN6472_c0_g1_i XP_026485871.1 protein phosphatase Mpk3-like 13037.EHJ70633 9.p1 [Vanessa tameamea] tr|A0A2H1WCM0|A0A2H1WCM0 _SPOFR SFRICE032389.2 TRINITY_DN54605_c0_g1 tr|A0A2H1WCM0|A0A2H1WCM0 OS=Spodoptera frugiperda 34740.HMEL007941-PA _i2.p1 _SPOFR OX=7108 GN=SFRICE032389.2 PE=4 SV=1 XP_026492014.1 phenoloxidase- TRINITY_DN246_c0_g2_i2 XP_026492014.1 activating enzyme-like [Vanessa 13037.EHJ73646 .p1 tameamea] tr|A0A1B0YTK3|A0A1B0YTK3_C TRINITY_DN954_c0_g1_i2 tr|A0A1B0YTK3|A0A1B0YTK3_C 7091.BGIBMGA001458- HISP ERK1 OS=Chilo suppressalis .p1 HISP TA OX=168631 PE=2 SV=1 XP_026489508.1 protein TRINITY_DN2490_c0_g1_i 7091.BGIBMGA010476- XP_026489508.1 croquemort-like [Vanessa 11.p1 TA tameamea] XP_026485197.1 transcription TRINITY_DN2500_c0_g1_i XP_026485197.1 factor BCFI-like isoform X1 34740.HMEL007452-PA 10.p1 [Vanessa tameamea] tr|A0A194RCY1|A0A194RCY1_P TRINITY_DN104210_c0_g tr|A0A194RCY1|A0A194RCY1_P APMA Hemicentin-1 OS=Papilio 13037.EHJ66252 1_i1.p1 APMA machaon OX=76193 GN=RR48_09351 PE=4 SV=1 117

TRINITY_DN25110_c0_g1 13037.EHJ70597 _i10.p2 TRINITY_DN25901_c0_g1 XP_026496789.1 short XP_026496789.1 34740.HMEL016788-PA _i1.p1 neuropeptide F [Vanessa tameamea] XP_032528679.1 insulin-like TRINITY_DN5461_c0_g1_i XP_032528679.1 receptor [Danaus plexippus 13037.EHJ65074 2.p1 plexippus] tr|A0A212FKL4|A0A212FKL4_DA TRINITY_DN11178_c0_g1 tr|A0A212FKL4|A0A212FKL4_DA NPL Anon-2 OS=Danaus plexippus 13037.EHJ78324 _i1.p1 NPL plexippus OX=278856 GN=KGM_208365 PE=4 SV=1 tr|A0A2H1V891|A0A2H1V891_SP OFR Tubulin alpha chain TRINITY_DN10319_c0_g1 tr|A0A2H1V891|A0A2H1V891_SP OS=Spodoptera frugiperda 34740.HMEL014785-PA _i1.p1 OFR OX=7108 GN=SFRICE_000539 PE=3 SV=1 tr|A0A212EWZ8|A0A212EWZ8_D TRINITY_DN3440_c0_g1_i tr|A0A212EWZ8|A0A212EWZ8_D ANPL Dorsal OS=Danaus 13037.EHJ69235 5.p1 ANPL plexippus plexippus OX=278856 GN=KGM_202775 PE=4 SV=1 tr|A0A2H1VHA2|A0A2H1VHA2_ SPOFR SFRICE_020488 TRINITY_DN603_c0_g1_i3 tr|A0A2H1VHA2|A0A2H1VHA2_ OS=Spodoptera frugiperda 13037.EHJ63782 6.p1 SPOFR OX=7108 GN=SFRICE_020488 PE=4 SV=1 XP_026499565.1 condensin TRINITY_DN12611_c0_g1 XP_026499565.1 complex subunit 1 isoform X2 34740.HMEL017572-PA _i4.p1 [Vanessa tameamea] tr|A0A5E4PTP9|A0A5E4PTP9_9N EOP Homeobox domain-containing TRINITY_DN6435_c0_g1_i tr|A0A5E4PTP9|A0A5E4PTP9_9N protein OS=Leptidea sinapis 34740.HMEL025000-PA 10.p1 EOP OX=189913 GN=LSINAPIS_LOCUS1829 PE=3 SV=1 tr|A0A212EKP7|A0A212EKP7_DA TRINITY_DN2652_c0_g1_i tr|A0A212EKP7|A0A212EKP7_DA NPL Kinase ASK1 OS=Danaus 34740.HMEL006581-PA 4.p1 NPL plexippus plexippus OX=278856 GN=KGM_201965 PE=4 SV=1 XP_026486850.1 prolactin- TRINITY_DN26649_c0_g1 7091.BGIBMGA012114- XP_026486850.1 releasing peptide receptor-like _i2.p1 TA [Vanessa tameamea] tr|A0A212ESK0|A0A212ESK0_DA TRINITY_DN2668_c1_g1_i tr|A0A212ESK0|A0A212ESK0_DA NPL Hemolin OS=Danaus 13037.EHJ68008 1.p1 NPL plexippus plexippus OX=278856 GN=KGM_205240 PE=4 SV=1 tr|A0A212F9V4|A0A212F9V4_DA NPL Phosphatidylinositol 3-kinase TRINITY_DN26719_c0_g1 tr|A0A212F9V4|A0A212F9V4_DA catalytic subunit type 3 OS=Danaus 13037.EHJ74132 _i3.p1 NPL plexippus plexippus OX=278856 GN=KGM_201093 PE=3 SV=1 XP_028178619.1 uncharacterized TRINITY_DN26799_c0_g1 7091.BGIBMGA011034- XP_028178619.1 protein LOC114366053 [Ostrinia _i1.p1 TA furnacalis] tr|A0A212FIG5|A0A212FIG5_DA NPL mRNA export protein TRINITY_DN5027_c0_g1_i tr|A0A212FIG5|A0A212FIG5_DA OS=Danaus plexippus plexippus 34740.HMEL008585-PA 10.p1 NPL OX=278856 GN=KGM_203528 PE=4 SV=1 118

tr|A0A5E4QAZ7|A0A5E4QAZ7_9 NEOP PseudoU_synth_2 domain- TRINITY_DN6169_c1_g1_i tr|A0A5E4QAZ7|A0A5E4QAZ7_9 containing protein OS=Leptidea 13037.EHJ68106 2.p2 NEOP sinapis OX=189913 GN=LSINAPIS_LOCUS6232 PE=4 SV=1 UniRef90_Q95W05 DSCAM splice variant 4.12 (Fragment) n=1 TRINITY_DN30171_c0_g1 UniRef90_Q95W05 Tax=Drosophila yakuba 132113.XP_003492247.1 _i1.p1 TaxID=7245 RepID=Q95W05_DROYA TRINITY_DN2696_c0_g1_i XP_022128344.1 PAX-interacting XP_022128344.1 34740.HMEL008670-PA 6.p1 protein 1-like, partial [Pieris rapae] tr|A0A212ESM0|A0A212ESM0_D ANPL Beta-1,3-glucan recognition TRINITY_DN1140_c0_g1_i tr|A0A212ESM0|A0A212ESM0_D protein OS=Danaus plexippus 34740.HMEL003270-PA 1.p1 ANPL plexippus OX=278856 GN=KGM_212963 PE=4 SV=1 XP_026501488.1 uncharacterized TRINITY_DN2974_c0_g1_i XP_026501488.1 protein LOC113404726 isoform X1 34740.HMEL008635-PA 10.p1 [Vanessa tameamea] TRINITY_DN5308_c0_g1_i XP_026500689.1 alpha- 7091.BGIBMGA012267- XP_026500689.1 1.p1 mannosidase 2 [Vanessa tameamea] TA XP_026494184.1 CLIP domain- TRINITY_DN40491_c0_g1 XP_026494184.1 containing serine protease 2-like 34740.HMEL016483-PA _i3.p1 [Vanessa tameamea] TRINITY_DN27150_c0_g1 XP_026754595.2 PAX-interacting XP_026754595.2 13037.EHJ79047 _i1.p1 protein 1-like [Galleria mellonella] tr|A0A2H1VQQ8|A0A2H1VQQ8_ SPOFR SFRICE_003562 TRINITY_DN2805_c2_g1_i tr|A0A2H1VQQ8|A0A2H1VQQ8_ OS=Spodoptera frugiperda 13037.EHJ63743 2.p1 SPOFR OX=7108 GN=SFRICE_003562 PE=3 SV=1 tr|A0A517BDU4|A0A517BDU4_H TRINITY_DN2759_c0_g1_i tr|A0A517BDU4|A0A517BDU4_H ELME Kurtz OS=Heliconius 13037.EHJ69141 10.p1 ELME melpomene OX=34740 PE=2 SV=1 tr|A0A2A4JJF7|A0A2A4JJF7_HEL VI ENTH domain-containing TRINITY_DN2145_c1_g1_i tr|A0A2A4JJF7|A0A2A4JJF7_HEL protein OS=Heliothis virescens 7165.AGAP010087-PA 8.p1 VI OX=7102 GN=B5V51_1012 PE=4 SV=1 sp|P31398|HEMO_MANSE TRINITY_DN276_c1_g4_i1 sp|P31398|HEMO_MANSE Hemolin OS=Manduca sexta 13037.EHJ68008 .p1 OX=7130 PE=2 SV=2 tr|A0A212EQ34|A0A212EQ34_DA NPL Ecdysone receptor B1 isoform TRINITY_DN4610_c0_g1_i tr|A0A212EQ34|A0A212EQ34_DA OS=Danaus plexippus plexippus 13037.EHJ66465 20.p1 NPL OX=278856 GN=KGM_208114 PE=3 SV=1 XP_026499246.1 heparan sulfate 2- TRINITY_DN17110_c0_g2 XP_026499246.1 O-sulfotransferase pipe [Vanessa 13037.EHJ65994 _i1.p1 tameamea] tr|A0A212FBJ8|A0A212FBJ8_DA NPL TAK1-associated binding TRINITY_DN4137_c0_g1_i tr|A0A212FBJ8|A0A212FBJ8_DA protein 2 isoform A OS=Danaus 13037.EHJ74734 1.p1 NPL plexippus plexippus OX=278856 GN=KGM_200977 PE=4 SV=1 119

XP_026492851.1 protein spaetzle- TRINITY_DN2802_c0_g1_i XP_026492851.1 like isoform X1 [Vanessa 13037.EHJ70909 1.p1 tameamea] TRINITY_DN152158_c0_g 34740.HMEL012666-PA 1_i2.p1 tr|A0A5E4QYK4|A0A5E4QYK4_9 NEOP Protein kinase domain- TRINITY_DN2813_c0_g1_i tr|A0A5E4QYK4|A0A5E4QYK4_9 containing protein OS=Leptidea 13037.EHJ74929 1.p1 NEOP sinapis OX=189913 GN=LSINAPIS_LOCUS12564 PE=3 SV=1 tr|A0A5E4QXB3|A0A5E4QXB3_9 NEOP Protein kinase domain- TRINITY_DN2813_c0_g1_i tr|A0A5E4QXB3|A0A5E4QXB3_9 containing protein OS=Leptidea 13037.EHJ74929 7.p1 NEOP sinapis OX=189913 GN=LSINAPIS_LOCUS12564 PE=4 SV=1 XP_026490449.1 eukaryotic TRINITY_DN3748_c0_g1_i XP_026490449.1 translation initiation factor 2-alpha 34740.HMEL004714-PA 2.p1 kinase 1-like [Vanessa tameamea] tr|A0A212F2L0|A0A212F2L0_DA NPL Ribosome biogenesis protein TRINITY_DN2838_c0_g1_i tr|A0A212F2L0|A0A212F2L0_DA BOP1 homolog OS=Danaus 34740.HMEL011695-PA 3.p1 NPL plexippus plexippus OX=278856 GN=KGM_213986 PE=3 SV=1 tr|A0A212ERW2|A0A212ERW2_D ANPL Non-specific TRINITY_DN4685_c0_g1_i tr|A0A212ERW2|A0A212ERW2_D serine/threonine protein kinase 13037.EHJ67195 3.p1 ANPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_206674 PE=4 SV=1 TRINITY_DN2832_c1_g1_i XP_026483724.1 cyclin-dependent XP_026483724.1 13037.EHJ75684 3.p1 kinase 6 [Vanessa tameamea] TRINITY_DN5776_c1_g1_i 13037.EHJ74876 23.p1 tr|A0A212F2L0|A0A212F2L0_DA NPL Ribosome biogenesis protein TRINITY_DN2838_c0_g1_i tr|A0A212F2L0|A0A212F2L0_DA BOP1 homolog OS=Danaus 34740.HMEL011695-PA 4.p1 NPL plexippus plexippus OX=278856 GN=KGM_213986 PE=3 SV=1 tr|A0A2A4K0B1|A0A2A4K0B1_H ELVI Non-specific serine/threonine TRINITY_DN2844_c1_g1_i tr|A0A2A4K0B1|A0A2A4K0B1_H protein kinase OS=Heliothis 34740.HMEL011297-PA 31.p1 ELVI virescens OX=7102 GN=B5V51_6600 PE=3 SV=1 tr|A0A2A4K0B1|A0A2A4K0B1_H ELVI Non-specific serine/threonine TRINITY_DN2844_c1_g1_i tr|A0A2A4K0B1|A0A2A4K0B1_H protein kinase OS=Heliothis 34740.HMEL011297-PA 33.p1 ELVI virescens OX=7102 GN=B5V51_6600 PE=3 SV=1 TRINITY_DN2849_c2_g1_i XP_026500902.1 protein toll-like, XP_026500902.1 13037.EHJ63304 1.p1 partial [Vanessa tameamea] tr|A0A0N0PCV4|A0A0N0PCV4_P TRINITY_DN286_c2_g1_i5 tr|A0A0N0PCV4|A0A0N0PCV4_P APMA Adapter molecule Crk 13037.EHJ72169 .p1 APMA OS=Papilio machaon OX=76193 GN=RR48_04475 PE=4 SV=1 120

Q8N1I8.1 RecName: Full=Putative uncharacterized protein encoded by TRINITY_DN2134_c0_g2_i 9646.ENSAMEP0000000 Q8N1I8.1 CACTIN-AS1; AltName: 1.p2 6044 Full=Cactin antisense RNA 1 [Homo sapiens] tr|S4PKA2|S4PKA2_9NEOP TRINITY_DN2876_c0_g1_i Magnesium transporter protein 1 tr|S4PKA2|S4PKA2_9NEOP 13037.EHJ71501 2.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 tr|S4P8H1|S4P8H1_9NEOP TRINITY_DN28965_c0_g1 Inhibitor of apoptosis 2 (Fragment) tr|S4P8H1|S4P8H1_9NEOP 34740.HMEL002562-PA _i3.p1 OS=Pararge aegeria OX=116150 PE=4 SV=1 XP_026496515.1 translin- TRINITY_DN4097_c1_g1_i associated factor X-interacting XP_026496515.1 13037.EHJ64431 11.p1 protein 1-like isoform X1 [Vanessa tameamea] tr|A0A2A4JLP3|A0A2A4JLP3_HE LVI Med25 domain-containing TRINITY_DN215_c0_g1_i7 tr|A0A2A4JLP3|A0A2A4JLP3_HE protein OS=Heliothis virescens 34740.HMEL004110-PA 5.p1 LVI OX=7102 GN=B5V51_272 PE=4 SV=1 TRINITY_DN29107_c0_g1 XP_026495706.1 lysozyme-like XP_026495706.1 13037.EHJ63150 _i1.p1 [Vanessa tameamea] TRINITY_DN4644_c0_g1_i XP_026486699.1 protein serrate XP_026486699.1 13037.EHJ63211 2.p1 [Vanessa tameamea] TRINITY_DN2939_c1_g1_i 34740.HMEL003132-PA 1.p1 XP_023945335.1 LOW QUALITY TRINITY_DN2939_c1_g2_i PROTEIN: homeodomain- XP_023945335.1 34740.HMEL003132-PA 1.p1 interacting protein kinase 2 [Bicyclus anynana] XP_023945335.1 LOW QUALITY TRINITY_DN2939_c1_g2_i PROTEIN: homeodomain- XP_023945335.1 34740.HMEL003132-PA 7.p1 interacting protein kinase 2 [Bicyclus anynana] XP_023945335.1 LOW QUALITY TRINITY_DN2939_c1_g2_i PROTEIN: homeodomain- XP_023945335.1 34740.HMEL003132-PA 8.p1 interacting protein kinase 2 [Bicyclus anynana] PSS14293.1 Heat shock protein TRINITY_DN55080_c2_g1 PSS14293.1 Hsp90 family protein, partial 4081.Solyc12g015880.1.1 _i1.p2 [Actinidia chinensis var. chinensis] tr|A0A212EJA3|A0A212EJA3_DA NPL Tyrosine-protein phosphatase TRINITY_DN2956_c0_g1_i tr|A0A212EJA3|A0A212EJA3_DA non-receptor type 23 OS=Danaus 13037.EHJ64144 13.p1 NPL plexippus plexippus OX=278856 GN=KGM_210906 PE=4 SV=1 XP_026501588.1 echinoderm TRINITY_DN26538_c1_g1 microtubule-associated protein-like XP_026501588.1 13037.EHJ70574 _i1.p1 CG42247 isoform X3 [Vanessa tameamea] XP_026485179.1 serine/threonine- TRINITY_DN4406_c0_g1_i XP_026485179.1 protein kinase mig-15 isoform X3 13037.EHJ69742 6.p1 [Vanessa tameamea] XP_021206369.1 probable TRINITY_DN29682_c0_g1 XP_021206369.1 cytochrome P450 49a1 [Bombyx 34740.HMEL007941-PA _i1.p1 mori] 121

XP_026499133.1 ubiquitin TRINITY_DN6053_c2_g1_i XP_026499133.1 carboxyl-terminal hydrolase 36 13037.EHJ71919 1.p1 [Vanessa tameamea] TRINITY_DN29885_c0_g1 VVD03338.1 unnamed protein VVD03338.1 13037.EHJ69065 _i6.p1 product, partial [Leptidea sinapis] TRINITY_DN2992_c0_g2_i XP_026495999.1 lysozyme-like XP_026495999.1 34740.HMEL016024-PA 4.p1 isoform X1 [Vanessa tameamea] XP_018334882.1 evolutionarily TRINITY_DN3083_c0_g1_i conserved signaling intermediate in XP_018334882.1 13037.EHJ72448 5.p2 Toll pathway, mitochondrial isoform X2 [Agrilus planipennis] XP_026491188.1 anaphase- TRINITY_DN3388_c0_g1_i XP_026491188.1 promoting complex subunit 4 34740.HMEL002243-PA 1.p1 [Vanessa tameamea] tr|S4PBQ2|S4PBQ2_9NEOP Rac1 TRINITY_DN3012_c2_g1_i tr|S4PBQ2|S4PBQ2_9NEOP OS=Pararge aegeria OX=116150 34740.HMEL007742-PA 4.p1 PE=4 SV=1 tr|A0A3S2LQ81|A0A3S2LQ81_CH ISP Protein kinase domain- TRINITY_DN3012_c3_g1_i tr|A0A3S2LQ81|A0A3S2LQ81_CH containing protein (Fragment) 13037.EHJ76637 2.p1 ISP OS=Chilo suppressalis OX=168631 GN=evm_013211 PE=4 SV=1 XP_026495417.1 E3 SUMO- TRINITY_DN3018_c0_g1_i XP_026495417.1 protein ligase PIAS3 isoform X1 34740.HMEL005634-PA 10.p1 [Vanessa tameamea] tr|I1VXL7|I1VXL7_BOMMO TRINITY_DN3018_c0_g1_i tr|I1VXL7|I1VXL7_BOMMO PIAS2 protein OS=Bombyx mori 13037.EHJ73473 2.p1 OX=7091 GN=PIAS2 PE=2 SV=1 tr|S4PGM8|S4PGM8_9NEOP TRINITY_DN30575_c0_g1 Phagocyte signaling-impaired tr|S4PGM8|S4PGM8_9NEOP 13037.EHJ79192 _i4.p1 protein OS=Pararge aegeria OX=116150 PE=4 SV=1 tr|A0A194PV73|A0A194PV73_PA TRINITY_DN3063_c0_g1_i tr|A0A194PV73|A0A194PV73_PA PXU NF-kappa-B inhibitor cactus 34740.HMEL013379-PA 1.p1 PXU OS=Papilio xuthus OX=66420 GN=RR46_05013 PE=4 SV=1 XP_013200422.1 PREDICTED: E3 TRINITY_DN3080_c1_g1_i XP_013200422.1 ubiquitin-protein ligase Su(dx)-like 34740.HMEL006816-PA 1.p1 [Amyelois transitella] XP_026492824.1 interleukin-1 TRINITY_DN7851_c0_g1_i XP_026492824.1 receptor-associated kinase 4-like 13037.EHJ70951 1.p1 [Vanessa tameamea] XP_026488292.1 evolutionarily TRINITY_DN3083_c0_g1_i conserved signaling intermediate in XP_026488292.1 34740.HMEL008262-PA 2.p1 Toll pathway, mitochondrial [Vanessa tameamea] UniRef90_H9J371 Uncharacterized TRINITY_DN3087_c0_g1_i protein n=1 Tax=Bombyx mori 7091.BGIBMGA001303- UniRef90_H9J371 18.p1 TaxID=7091 TA RepID=H9J371_BOMMO TRINITY_DN8735_c0_g1_i XP_026500469.1 protein NDRG3 XP_026500469.1 34740.HMEL013050-PA 20.p1 isoform X6 [Vanessa tameamea] TRINITY_DN1702_c0_g1_i XP_026495525.1 integrin beta-nu XP_026495525.1 13037.EHJ75539 1.p1 [Vanessa tameamea] XP_026489627.1 CLIP domain- TRINITY_DN379_c0_g1_i3 XP_026489627.1 containing serine protease 2 isoform 13037.EHJ78263 9.p1 X1 [Vanessa tameamea] 122

XP_026501308.1 peptidoglycan- TRINITY_DN3100_c0_g2_i XP_026501308.1 recognition protein LB-like 13037.EHJ79102 5.p1 [Vanessa tameamea] XP_026494208.1 CLIP domain- TRINITY_DN3103_c0_g1_i XP_026494208.1 containing serine protease 2-like 13037.EHJ76005 1.p1 [Vanessa tameamea] tr|A0A5E4PRK1|A0A5E4PRK1_9 NEOP Spaetzle domain-containing TRINITY_DN13265_c0_g1 tr|A0A5E4PRK1|A0A5E4PRK1_9 protein OS=Leptidea sinapis 13037.EHJ75513 _i6.p1 NEOP OX=189913 GN=LSINAPIS_LOCUS1889 PE=4 SV=1 TRINITY_DN1685_c3_g1_i XP_026500315.1 modular serine XP_026500315.1 34740.HMEL013253-PA 6.p1 protease-like [Vanessa tameamea] tr|A0A437BQW9|A0A437BQW9_ CHISP PDZ domain-containing TRINITY_DN3112_c1_g1_i tr|A0A437BQW9|A0A437BQW9_ protein OS=Chilo suppressalis 13037.EHJ70644 4.p1 CHISP OX=168631 GN=evm_002461 PE=4 SV=1 TRINITY_DN3115_c0_g1_i XP_012552529.1 titin-like, partial XP_012552529.1 13037.EHJ65548 1.p2 [Bombyx mori] UniRef90_UPI0012319266 hypothetical protein n=1 TRINITY_DN3115_c0_g1_i UniRef90_UPI0012319266 Tax=Pseudomonas aeruginosa 13037.EHJ65548 25.p3 TaxID=287 RepID=UPI0012319266 UniRef90_A0A0T6B6Y0 Immunoglobulin (Fragment) n=1 TRINITY_DN3115_c1_g2_i UniRef90_A0A0T6B6Y0 Tax=Oryctes borbonicus 13037.EHJ65548 18.p1 TaxID=1629725 RepID=A0A0T6B6Y0_9SCAR tr|A0A212FHF8|A0A212FHF8_DA NPL Broad-complex isoform Z1 TRINITY_DN5219_c0_g1_i tr|A0A212FHF8|A0A212FHF8_DA OS=Danaus plexippus plexippus 13037.EHJ77002 11.p1 NPL OX=278856 GN=KGM_207435 PE=4 SV=1 tr|A0A2H1W1R5|A0A2H1W1R5_S POFR SFRICE_030507 (Fragment) TRINITY_DN31287_c0_g1 tr|A0A2H1W1R5|A0A2H1W1R5_S OS=Spodoptera frugiperda 34740.HMEL002495-PA _i1.p1 POFR OX=7108 GN=SFRICE_030507 PE=4 SV=1 TRINITY_DN3144_c0_g2_i XP_023939372.1 lysozyme-like XP_023939372.1 13037.EHJ77779 7.p1 [Bicyclus anynana] tr|A0A212EUK9|A0A212EUK9_D ANPL C-type lectin domain- TRINITY_DN6938_c0_g1_i tr|A0A212EUK9|A0A212EUK9_D containing protein OS=Danaus 13037.EHJ68272 2.p1 ANPL plexippus plexippus OX=278856 GN=KGM_213295 PE=4 SV=1 tr|A0A212EKL9|A0A212EKL9_D ANPL Defective in cullin TRINITY_DN6626_c0_g1_i tr|A0A212EKL9|A0A212EKL9_D neddylation protein OS=Danaus 34740.HMEL005396-PA 1.p1 ANPL plexippus plexippus OX=278856 GN=KGM_204340 PE=4 SV=1 tr|A0A212ET32|A0A212ET32_DA TRINITY_DN20565_c0_g1 tr|A0A212ET32|A0A212ET32_DA NPL Serpin-27 OS=Danaus 34740.HMEL006662-PA _i9.p1 NPL plexippus plexippus OX=278856 GN=KGM_202595 PE=3 SV=1 123

KPJ13180.1 Ankyrin repeat TRINITY_DN3295_c1_g1_i KPJ13180.1 domain-containing protein 17 13037.EHJ73802 3.p1 [Papilio machaon] tr|A0A212ERZ7|A0A212ERZ7_DA NPL Tyrosine-protein kinase TRINITY_DN3163_c0_g1_i tr|A0A212ERZ7|A0A212ERZ7_DA OS=Danaus plexippus plexippus 13037.EHJ67223 11.p1 NPL OX=278856 GN=KGM_207312 PE=3 SV=1 tr|A0A212EJ91|A0A212EJ91_DAN PL Transcription initiation factor TRINITY_DN3173_c0_g1_i tr|A0A212EJ91|A0A212EJ91_DAN TFIID subunit 1 OS=Danaus 13037.EHJ64104 3.p1 PL plexippus plexippus OX=278856 GN=KGM_200534 PE=4 SV=1 tr|A0A212FEK8|A0A212FEK8_DA NPL G_PROTEIN_RECEP_F3_4 TRINITY_DN31949_c0_g1 tr|A0A212FEK8|A0A212FEK8_DA domain-containing protein 34740.HMEL012400-PA _i3.p1 NPL OS=Danaus plexippus plexippus OX=278856 GN=KGM_201285 PE=4 SV=1 tr|A0A2H1W4G8|A0A2H1W4G8_ SPOFR Defective in cullin TRINITY_DN4829_c0_g1_i tr|A0A2H1W4G8|A0A2H1W4G8_ neddylation protein (Fragment) 34740.HMEL010069-PA 10.p1 SPOFR OS=Spodoptera frugiperda OX=7108 GN=SFRICE_005752 PE=4 SV=1 tr|Q60FS2|Q60FS2_PLUXY TRINITY_DN3279_c0_g1_i Receptor for activated protein 7091.BGIBMGA007477- tr|Q60FS2|Q60FS2_PLUXY 1.p2 kinase C homolog OS=Plutella TA xylostella OX=51655 PE=2 SV=1 tr|A0A212ELY3|A0A212ELY3_DA NPL Cyclin dependent kinase 2 TRINITY_DN3295_c0_g1_i tr|A0A212ELY3|A0A212ELY3_DA OS=Danaus plexippus plexippus 13037.EHJ65237 1.p1 NPL OX=278856 GN=KGM_210561 PE=3 SV=1 tr|A0A2A4JS51|A0A2A4JS51_HEL VI Importin N-terminal domain- TRINITY_DN9157_c0_g1_i tr|A0A2A4JS51|A0A2A4JS51_HEL containing protein OS=Heliothis 13037.EHJ74083 2.p1 VI virescens OX=7102 GN=B5V51_13000 PE=4 SV=1 UniRef90_I4DRH9 TRINITY_DN4771_c0_g2_i Uncharacterized protein n=1 UniRef90_I4DRH9 34740.HMEL013337-PA 10.p4 Tax=Papilio polytes TaxID=76194 RepID=I4DRH9_PAPPL UniRef90_B6A8H4 Titin-like protein (Fragment) n=1 TRINITY_DN2039_c0_g1_i UniRef90_B6A8H4 Tax=Helicoverpa armigera 13037.EHJ65548 12.p4 TaxID=29058 RepID=B6A8H4_HELAM tr|A0A2A4JW42|A0A2A4JW42_H ELVI C-type lectin domain- TRINITY_DN33395_c0_g1 tr|A0A2A4JW42|A0A2A4JW42_H containing protein OS=Heliothis 34740.HMEL017185-PA _i4.p1 ELVI virescens OX=7102 GN=B5V51_10869 PE=4 SV=1 XP_026483309.1 RAC TRINITY_DN3369_c0_g1_i XP_026483309.1 serine/threonine-protein kinase 13037.EHJ69136 21.p1 isoform X1 [Vanessa tameamea] TRINITY_DN8735_c0_g1_i XP_026500469.1 protein NDRG3 XP_026500469.1 34740.HMEL013050-PA 4.p1 isoform X6 [Vanessa tameamea] 124

tr|Q4AED5|Q4AED5_BOMMO TRINITY_DN3392_c0_g1_i tr|Q4AED5|Q4AED5_BOMMO Protein kinase C OS=Bombyx mori 7222.FBpp0154708 2.p1 OX=7091 GN=aPKC PE=2 SV=1 tr|A0A2A4JHQ5|A0A2A4JHQ5_H ELVI Homeobox domain- TRINITY_DN16639_c0_g1 tr|A0A2A4JHQ5|A0A2A4JHQ5_H containing protein OS=Heliothis 34740.HMEL006180-PA _i1.p1 ELVI virescens OX=7102 GN=B5V51_2014 PE=4 SV=1 XP_026488899.1 bicaudal D- TRINITY_DN128_c2_g1_i1 7091.BGIBMGA013228- XP_026488899.1 related protein homolog [Vanessa .p1 TA tameamea] TRINITY_DN34130_c0_g1 XP_031764774.1 protein bicaudal XP_031764774.1 34740.HMEL016019-PA _i1.p1 C-like [Galleria mellonella] TRINITY_DN5538_c0_g1_i KOB52327.1 mRNA export protein, KOB52327.1 34740.HMEL008585-PA 3.p1 partial [Operophtera brumata] UniRef90_A0A0L7LRA9 Uncharacterized protein n=1 TRINITY_DN134025_c0_g UniRef90_A0A0L7LRA9 Tax=Operophtera brumata 34740.HMEL013671-PA 1_i1.p1 TaxID=104452 RepID=A0A0L7LRA9_9NEOP tr|A0A212EWZ8|A0A212EWZ8_D TRINITY_DN3440_c0_g1_i tr|A0A212EWZ8|A0A212EWZ8_D ANPL Dorsal OS=Danaus 13037.EHJ69235 11.p1 ANPL plexippus plexippus OX=278856 GN=KGM_202775 PE=4 SV=1 XP_026483228.1 ras-related protein TRINITY_DN3443_c0_g1_i XP_026483228.1 Ral-a isoform X1 [Vanessa 13037.EHJ75874 3.p1 tameamea] XP_026501735.1 probable ATP- TRINITY_DN34493_c0_g1 XP_026501735.1 dependent RNA helicase DHX35 34740.HMEL022585-PA _i3.p1 [Vanessa tameamea] XP_026484577.1 antichymotrypsin- TRINITY_DN3454_c0_g1_i XP_026484577.1 2-like isoform X1 [Vanessa 34740.HMEL006260-PA 7.p1 tameamea] UniRef90_M7ZVH4 31 kDa TRINITY_DN79525_c0_g1 ribonucleoprotein, chloroplastic n=3 29760.VIT_14s0066g0014 UniRef90_M7ZVH4 _i1.p1 Tax=Triticinae TaxID=1648030 0.t01 RepID=M7ZVH4_TRIUA tr|A0A212FKS5|A0A212FKS5_DA TRINITY_DN3461_c0_g1_i tr|A0A212FKS5|A0A212FKS5_DA NPL Sickie OS=Danaus plexippus 13037.EHJ78388 6.p1 NPL plexippus OX=278856 GN=KGM_204491 PE=4 SV=1 TRINITY_DN34622_c2_g1 PCG72075.1 hypothetical protein 7091.BGIBMGA000603- PCG72075.1 _i1.p1 B5V51_1185 [Heliothis virescens] TA UniRef90_A0A5E4R2S2 WD_REPEATS_REGION domain- TRINITY_DN15729_c0_g1 containing protein n=3 UniRef90_A0A5E4R2S2 34740.HMEL016524-PA _i11.p1 Tax=Leptidea sinapis TaxID=189913 RepID=A0A5E4R2S2_9NEOP tr|A0A5E4QDU0|A0A5E4QDU0_9 NEOP Protein kinase domain- TRINITY_DN3489_c0_g2_i tr|A0A5E4QDU0|A0A5E4QDU0_9 containing protein OS=Leptidea 7091.BGIBMGA003607- 1.p1 NEOP sinapis OX=189913 TA GN=LSINAPIS_LOCUS7001 PE=4 SV=1 TRINITY_DN3511_c0_g1_i XP_026499312.1 titin-like [Vanessa XP_026499312.1 13037.EHJ65548 20.p1 tameamea] 125

tr|A0A212F1I3|A0A212F1I3_DAN PL NIMA-related kinase 8 TRINITY_DN35446_c6_g1 tr|A0A212F1I3|A0A212F1I3_DAN OS=Danaus plexippus plexippus 13037.EHJ70997 _i1.p1 PL OX=278856 GN=KGM_205758 PE=3 SV=1 tr|A0A5E4QA97|A0A5E4QA97_9 NEOP Mitogen-activated protein TRINITY_DN19972_c0_g1 tr|A0A5E4QA97|A0A5E4QA97_9 kinase OS=Leptidea sinapis 13037.EHJ76051 _i7.p1 NEOP OX=189913 GN=LSINAPIS_LOCUS6957 PE=4 SV=1 tr|A0A2H1WUA9|A0A2H1WUA9_ SPOFR SFRICE012688.2 TRINITY_DN3552_c0_g1_i tr|A0A2H1WUA9|A0A2H1WUA9_ (Fragment) OS=Spodoptera 13037.EHJ65184 1.p1 SPOFR frugiperda OX=7108 GN=SFRICE012688.2 PE=4 SV=1 tr|A0A212EL17|A0A212EL17_DA NPL Non-specific serine/threonine TRINITY_DN3568_c0_g1_i tr|A0A212EL17|A0A212EL17_DA protein kinase OS=Danaus 34740.HMEL015167-PA 7.p1 NPL plexippus plexippus OX=278856 GN=KGM_204301 PE=4 SV=1 XP_026501385.1 DNA replication TRINITY_DN12292_c0_g1 ATP-dependent helicase/nuclease XP_026501385.1 34740.HMEL006856-PA _i1.p1 DNA2 isoform X1 [Vanessa tameamea] tr|A0A194RSC1|A0A194RSC1_PA PMA Nuclear factor NF-kappa-B TRINITY_DN3986_c0_g2_i tr|A0A194RSC1|A0A194RSC1_PA p110 subunit OS=Papilio machaon 34740.HMEL008894-PA 2.p1 PMA OX=76193 GN=RR48_12477 PE=4 SV=1 tr|A0A212FKX9|A0A212FKX9_D TRINITY_DN1047_c0_g1_i tr|A0A212FKX9|A0A212FKX9_D ANPL Attacin OS=Danaus 13037.EHJ78463 3.p1 ANPL plexippus plexippus OX=278856 GN=KGM_213997 PE=4 SV=1 XP_026496596.1 SHC- TRINITY_DN3610_c0_g1_i XP_026496596.1 transforming protein 1 [Vanessa 13037.EHJ69724 13.p1 tameamea] tr|A0A194PII4|A0A194PII4_PAPX U TATA-binding protein-associated TRINITY_DN3627_c0_g1_i tr|A0A194PII4|A0A194PII4_PAPX factor 172 OS=Papilio xuthus 13037.EHJ67216 3.p1 U OX=66420 GN=RR46_14348 PE=4 SV=1 TRINITY_DN3637_c1_g2_i 13037.EHJ73477 2.p2 tr|A0A2H1VYV5|A0A2H1VYV5_ SPOFR SFRICE_031476 TRINITY_DN12403_c0_g1 tr|A0A2H1VYV5|A0A2H1VYV5_ (Fragment) OS=Spodoptera 13037.EHJ73373 _i4.p2 SPOFR frugiperda OX=7108 GN=SFRICE_031476 PE=4 SV=1 TRINITY_DN36408_c0_g1 XP_026497736.1 lysozyme XP_026497736.1 34740.HMEL002880-PA _i3.p1 [Vanessa tameamea] tr|A0A2H1V1C7|A0A2H1V1C7_S POFR SFRICE_003046 TRINITY_DN4203_c0_g1_i tr|A0A2H1V1C7|A0A2H1V1C7_S OS=Spodoptera frugiperda 13037.EHJ67611 1.p1 POFR OX=7108 GN=SFRICE_003046 PE=3 SV=1 126

tr|A0A437BKW2|A0A437BKW2_ CHISP Transcription elongation TRINITY_DN3662_c0_g1_i tr|A0A437BKW2|A0A437BKW2_ factor spt6 OS=Chilo suppressalis 34740.HMEL015312-PA 1.p1 CHISP OX=168631 GN=evm_004485 PE=3 SV=1 tr|A0A212FMW2|A0A212FMW2_ TRINITY_DN3128_c0_g1_i tr|A0A212FMW2|A0A212FMW2_ DANPL Merlin OS=Danaus 13037.EHJ79218 10.p1 DANPL plexippus plexippus OX=278856 GN=KGM_207092 PE=4 SV=1 tr|S4PMD3|S4PMD3_9NEOP TRINITY_DN4295_c0_g1_i tr|S4PMD3|S4PMD3_9NEOP Translin OS=Pararge aegeria 34740.HMEL011178-PA 1.p1 OX=116150 PE=4 SV=1 XP_026490768.1 adenosine TRINITY_DN3699_c0_g1_i XP_026490768.1 deaminase 2-like [Vanessa 34740.HMEL004270-PA 2.p1 tameamea] tr|A0A194PGJ2|A0A194PGJ2_PAP TRINITY_DN3733_c0_g1_i tr|A0A194PGJ2|A0A194PGJ2_PAP XU Alpha-mannosidase OS=Papilio 13037.EHJ73787 1.p1 XU xuthus OX=66420 GN=RR46_13643 PE=3 SV=1 tr|A0A212FAI1|A0A212FAI1_DAN PL Male-specific lethal 3 TRINITY_DN5776_c2_g1_i tr|A0A212FAI1|A0A212FAI1_DAN OS=Danaus plexippus plexippus 13037.EHJ74368 1.p1 PL OX=278856 GN=KGM_204979 PE=4 SV=1 tr|H9JND5|H9JND5_BOMMO TIR TRINITY_DN10155_c0_g1 domain-containing protein tr|H9JND5|H9JND5_BOMMO 34740.HMEL016226-PA _i11.p1 OS=Bombyx mori OX=7091 GN=101736604 PE=4 SV=1 tr|A0A194QBF4|A0A194QBF4_PA TRINITY_DN3761_c0_g1_i tr|A0A194QBF4|A0A194QBF4_PA PXU Membrane-associated protein 34740.HMEL022558-PA 2.p1 PXU Hem OS=Papilio xuthus OX=66420 GN=RR46_02800 PE=4 SV=1 tr|A0A5E4Q9S6|A0A5E4Q9S6_9N EOP SERPIN domain-containing TRINITY_DN37782_c0_g1 tr|A0A5E4Q9S6|A0A5E4Q9S6_9N protein OS=Leptidea sinapis 34740.HMEL007592-PA _i1.p1 EOP OX=189913 GN=LSINAPIS_LOCUS5986 PE=3 SV=1 tr|A0A212EWP1|A0A212EWP1_D ANPL Dedicator of cytokinesis TRINITY_DN3784_c0_g1_i tr|A0A212EWP1|A0A212EWP1_D protein 2 OS=Danaus plexippus 13037.EHJ69065 3.p1 ANPL plexippus OX=278856 GN=KGM_201862 PE=3 SV=1 TRINITY_DN37934_c0_g1 XP_026499659.1 dual oxidase XP_026499659.1 34740.HMEL017581-PA _i1.p1 isoform X1 [Vanessa tameamea] tr|H9JMW7|H9JMW7_BOMMO TRINITY_DN37971_c0_g1 Uncharacterized protein tr|H9JMW7|H9JMW7_BOMMO 34740.HMEL005137-PA _i2.p1 OS=Bombyx mori OX=7091 PE=4 SV=1 TRINITY_DN3797_c0_g1_i ETN62353.1 phospholipase c beta ETN62353.1 7165.AGAP001936-PA 4.p1 [Anopheles darlingi] tr|S4NRU2|S4NRU2_9NEOP TRINITY_DN3798_c1_g1_i Ragulator complex protein tr|S4NRU2|S4NRU2_9NEOP 13037.EHJ77469 3.p1 LAMTOR1 OS=Pararge aegeria OX=116150 PE=4 SV=1 XP_026489628.1 CLIP domain- TRINITY_DN379_c0_g1_i4 XP_026489628.1 containing serine protease 2 isoform 13037.EHJ78263 8.p1 X2 [Vanessa tameamea] 127

tr|A0A2H1WT43|A0A2H1WT43_S POFR Serine/threonine-protein TRINITY_DN3809_c0_g1_i tr|A0A2H1WT43|A0A2H1WT43_S kinase TOR OS=Spodoptera 13037.EHJ65032 2.p1 POFR frugiperda OX=7108 GN=SFRICE_000977 PE=3 SV=1 tr|S4PBX1|S4PBX1_9NEOP Cell TRINITY_DN38645_c0_g1 division cycle protein 27-like tr|S4PBX1|S4PBX1_9NEOP 34740.HMEL017908-PA _i1.p1 protein OS=Pararge aegeria OX=116150 PE=4 SV=1 tr|A0A2A4K8M1|A0A2A4K8M1_ HELVI CULLIN_2 domain- TRINITY_DN4746_c0_g1_i tr|A0A2A4K8M1|A0A2A4K8M1_ 7091.BGIBMGA005758- containing protein OS=Heliothis 2.p1 HELVI TA virescens OX=7102 GN=B5V51_8976 PE=3 SV=1 tr|A0A437BQC7|A0A437BQC7_C TRINITY_DN3822_c0_g1_i tr|A0A437BQC7|A0A437BQC7_C HISP Uncharacterized protein 34740.HMEL006786-PA 2.p1 HISP OS=Chilo suppressalis OX=168631 GN=evm_002747 PE=4 SV=1 TRINITY_DN23_c0_g1_i1. XP_026489430.1 ferritin-1, XP_026489430.1 34740.HMEL013441-PA p1 chloroplastic [Vanessa tameamea] TRINITY_DN3841_c0_g1_i XP_028172930.1 flotillin-2 isoform XP_028172930.1 13037.EHJ79160 1.p1 X1 [Ostrinia furnacalis] tr|Q19KB8|Q19KB8_BOMMO TRINITY_DN3848_c0_g1_i Ras-related GTP-binding protein 7091.BGIBMGA002209- tr|Q19KB8|Q19KB8_BOMMO 1.p1 Rab11 OS=Bombyx mori OX=7091 TA GN=733063 PE=2 SV=1 tr|A0A3S2NBT5|A0A3S2NBT5_C HISP C-type lectin domain- TRINITY_DN3868_c0_g1_i tr|A0A3S2NBT5|A0A3S2NBT5_C containing protein OS=Chilo 34740.HMEL003322-PA 2.p1 HISP suppressalis OX=168631 GN=evm_008474 PE=4 SV=1 UniRef90_UPI00083BE30D integrin alpha-PS2-like n=1 TRINITY_DN334_c1_g1_i4 UniRef90_UPI00083BE30D Tax=Nicrophorus vespilloides 7029.ACYPI009808-PA .p2 TaxID=110193 RepID=UPI00083BE30D tr|A0A5E4Q6I3|A0A5E4Q6I3_9NE OP SERPIN domain-containing TRINITY_DN1294_c1_g1_i tr|A0A5E4Q6I3|A0A5E4Q6I3_9NE protein OS=Leptidea sinapis 34740.HMEL007596-PA 10.p1 OP OX=189913 GN=LSINAPIS_LOCUS5987 PE=3 SV=1 UniRef90_A0A2G9GNH4 ML domain-containing protein n=1 TRINITY_DN91655_c0_g1 UniRef90_A0A2G9GNH4 Tax=Handroanthus impetiginosus 4155.Migut.J00746.1.p _i1.p1 TaxID=429701 RepID=A0A2G9GNH4_9LAMI tr|A0A212FKG9|A0A212FKG9_D ANPL Hemicentin-1 like protein TRINITY_DN10996_c0_g1 tr|A0A212FKG9|A0A212FKG9_D OS=Danaus plexippus plexippus 34740.HMEL002509-PA _i1.p1 ANPL OX=278856 GN=KGM_214568 PE=4 SV=1 XP_026494409.1 ETS-like protein TRINITY_DN3907_c0_g1_i XP_026494409.1 pointed isoform X1 [Vanessa 34740.HMEL004734-PA 5.p1 tameamea] XP_026492019.1 phenoloxidase- TRINITY_DN1887_c0_g1_i XP_026492019.1 activating enzyme-like isoform X4 34740.HMEL004369-PA 8.p1 [Vanessa tameamea] 128

tr|A0A212ESE0|A0A212ESE0_DA NPL Fibroblast growth factor TRINITY_DN3949_c0_g1_i tr|A0A212ESE0|A0A212ESE0_DA receptor OS=Danaus plexippus 13037.EHJ67396 2.p1 NPL plexippus OX=278856 GN=KGM_212041 PE=3 SV=1 TRINITY_DN14381_c1_g1 XP_026495603.1 piwi-like protein XP_026495603.1 34740.HMEL007215-PA _i10.p1 Ago3 [Vanessa tameamea] tr|A0A194PX42|A0A194PX42_PA TRINITY_DN4404_c0_g1_i tr|A0A194PX42|A0A194PX42_PA PXU Programmed cell death protein 13037.EHJ68480 15.p1 PXU 2 OS=Papilio xuthus OX=66420 GN=RR46_11013 PE=4 SV=1 XP_026500859.1 endoplasmic TRINITY_DN2632_c1_g1_i reticulum mannosyl-oligosaccharide 7091.BGIBMGA000564- XP_026500859.1 5.p1 1,2-alpha-mannosidase [Vanessa TA tameamea] tr|A0A194RSC1|A0A194RSC1_PA PMA Nuclear factor NF-kappa-B TRINITY_DN3986_c0_g1_i tr|A0A194RSC1|A0A194RSC1_PA p110 subunit OS=Papilio machaon 34740.HMEL008894-PA 27.p1 PMA OX=76193 GN=RR48_12477 PE=4 SV=1 UniRef90_A0A1E1W5B9 Uncharacterized protein n=1 TRINITY_DN3993_c0_g1_i UniRef90_A0A1E1W5B9 Tax=Pectinophora gossypiella 34740.HMEL002443-PA 4.p1 TaxID=13191 RepID=A0A1E1W5B9_PECGO TRINITY_DN40199_c0_g2 XP_026500363.1 cyclin-dependent 7091.BGIBMGA013453- XP_026500363.1 _i2.p1 kinase 2-like [Vanessa tameamea] TA TRINITY_DN40356_c0_g2 XP_026490081.1 trypsin epsilon- XP_026490081.1 13037.EHJ74018 _i2.p1 like [Vanessa tameamea] TRINITY_DN404_c2_g1_i1 13037.EHJ69742 .p1 XP_026501304.1 vacuolar protein TRINITY_DN4069_c0_g1_i XP_026501304.1 sorting-associated protein 33A 34740.HMEL016091-PA 4.p2 [Vanessa tameamea] tr|A0A2P1DM87|A0A2P1DM87_S TRINITY_DN4101_c0_g1_i tr|A0A2P1DM87|A0A2P1DM87_S POFR Pasha-PA OS=Spodoptera 34740.HMEL014155-PA 12.p1 POFR frugiperda OX=7108 PE=2 SV=1 tr|A0A2P1DM87|A0A2P1DM87_S TRINITY_DN4101_c0_g1_i tr|A0A2P1DM87|A0A2P1DM87_S POFR Pasha-PA OS=Spodoptera 34740.HMEL014155-PA 7.p1 POFR frugiperda OX=7108 PE=2 SV=1 XP_026490269.1 MD-2-related TRINITY_DN4112_c0_g1_i XP_026490269.1 lipid-recognition protein-like 34740.HMEL014315-PA 6.p1 [Vanessa tameamea] TRINITY_DN4118_c1_g1_i XP_023228916.1 integrin alpha- XP_023228916.1 13037.EHJ68673 1.p1 PS2-like [Centruroides sculpturatus] TRINITY_DN4118_c1_g1_i XP_023228916.1 integrin alpha- XP_023228916.1 13037.EHJ68673 12.p1 PS2-like [Centruroides sculpturatus] XP_026483862.1 CLIP-associating TRINITY_DN4119_c0_g1_i XP_026483862.1 protein isoform X2 [Vanessa 13037.EHJ66282 12.p1 tameamea] TRINITY_DN4119_c0_g2_i RVE43882.1 hypothetical protein RVE43882.1 13037.EHJ66282 9.p1 evm_011444 [Chilo suppressalis] tr|L7WW91|L7WW91_HELEA Dual specificity mitogen-activated TRINITY_DN4146_c0_g1_i tr|L7WW91|L7WW91_HELEA protein kinase kinase 6-like protein 34740.HMEL000039-PA 17.p1 OS=Heliconius erato OX=33431 PE=3 SV=1 129

tr|O77240|O77240_CHOFU TRINITY_DN4610_c0_g1_i Ecdysone receptor isoform A tr|O77240|O77240_CHOFU 13037.EHJ66465 22.p1 OS=Choristoneura fumiferana OX=7141 GN=EcR PE=2 SV=2 XP_026491162.1 transcriptional TRINITY_DN5118_c0_g2_i XP_026491162.1 enhancer factor TEF-1 isoform X1 13037.EHJ63836 2.p1 [Vanessa tameamea] tr|Q6I6N4|Q6I6N4_BOMMO TRINITY_DN5219_c0_g1_i Broad-Complex isoform Z4 tr|Q6I6N4|Q6I6N4_BOMMO 13037.EHJ77002 13.p1 OS=Bombyx mori OX=7091 GN=BRC PE=2 SV=1 XP_026490238.1 casein kinase I- TRINITY_DN4235_c0_g1_i XP_026490238.1 like isoform X4 [Vanessa 13037.EHJ67184 18.p1 tameamea] TRINITY_DN423_c1_g1_i2 XP_026489534.1 serine protease XP_026489534.1 34740.HMEL007596-PA .p1 inhibitor-like [Vanessa tameamea] XP_026487988.1 LOW QUALITY TRINITY_DN2046_c1_g1_i XP_026487988.1 PROTEIN: insulin receptor 13037.EHJ66806 3.p1 substrate 1 [Vanessa tameamea] tr|A0A2J8JEW3|A0A2J8JEW3_PA NTR Glyceraldehyde-3-phosphate TRINITY_DN42535_c0_g1 tr|A0A2J8JEW3|A0A2J8JEW3_PA 9593.ENSGGOP00000009 dehydrogenase OS=Pan troglodytes _i1.p1 NTR 331 OX=9598 GN=GAPDH PE=3 SV=1 TRINITY_DN90188_c0_g1 13037.EHJ76225 _i1.p1 tr|A0A0N0PBN9|A0A0N0PBN9_P APMA Tyrosine-protein TRINITY_DN4266_c0_g1_i tr|A0A0N0PBN9|A0A0N0PBN9_P phosphatase non-receptor type 14 34740.HMEL013547-PA 1.p1 APMA OS=Papilio machaon OX=76193 GN=RR48_01773 PE=4 SV=1 UniRef90_A0A1D2NM19 Afadin TRINITY_DN4282_c0_g1_i n=1 Tax=Orchesella cincta UniRef90_A0A1D2NM19 34740.HMEL018030-PA 2.p1 TaxID=48709 RepID=A0A1D2NM19_ORCCI tr|A0A2A4JKV6|A0A2A4JKV6_H ELVI ENTH domain-containing TRINITY_DN2145_c1_g1_i tr|A0A2A4JKV6|A0A2A4JKV6_H protein OS=Heliothis virescens 7165.AGAP010087-PA 11.p1 ELVI OX=7102 GN=B5V51_1012 PE=4 SV=1 TRINITY_DN21074_c0_g2 XP_026495858.1 lysozyme-like XP_026495858.1 13037.EHJ72779 _i2.p1 [Vanessa tameamea] XP_026490476.1 zinc finger TRINITY_DN4348_c0_g2_i XP_026490476.1 protein ush isoform X1 [Vanessa 13037.EHJ78015 1.p1 tameamea] tr|A0A2W1BIV1|A0A2W1BIV1_H ELAM BZIP domain-containing TRINITY_DN6017_c0_g1_i tr|A0A2W1BIV1|A0A2W1BIV1_H protein OS=Helicoverpa armigera 34740.HMEL009106-PA 20.p1 ELAM OX=29058 GN=HaOG209031 PE=4 SV=1 XP_026487717.1 anaphase- TRINITY_DN10331_c0_g1 XP_026487717.1 promoting complex subunit 5 13037.EHJ67970 _i1.p1 isoform X1 [Vanessa tameamea] XP_026485180.1 serine/threonine- TRINITY_DN4406_c0_g1_i XP_026485180.1 protein kinase mig-15 isoform X4 13037.EHJ69742 8.p1 [Vanessa tameamea] 130

tr|I4DR18|I4DR18_PAPPL Ste20- TRINITY_DN18948_c0_g1 tr|I4DR18|I4DR18_PAPPL like kinase OS=Papilio polytes 34740.HMEL009275-PA _i4.p1 OX=76194 PE=2 SV=1 TRINITY_DN4452_c0_g1_i RVE48336.1 hypothetical protein 7091.BGIBMGA013349- RVE48336.1 4.p2 evm_006996 [Chilo suppressalis] TA TRINITY_DN4455_c0_g2_i XP_026494818.1 caspase-8 XP_026494818.1 13037.EHJ75352 1.p1 [Vanessa tameamea] KPI99555.1 Mitogen-activated TRINITY_DN4957_c2_g1_i KPI99555.1 protein kinase kinase kinase 4 13037.EHJ76732 5.p1 [Papilio xuthus] tr|A0A2H1W5F4|A0A2H1W5F4_S POFR SFRICE_019184 TRINITY_DN4459_c0_g2_i tr|A0A2H1W5F4|A0A2H1W5F4_S 7091.BGIBMGA001044- OS=Spodoptera frugiperda 1.p1 POFR TA OX=7108 GN=SFRICE_019184 PE=4 SV=1 tr|A0A5E4Q9I9|A0A5E4Q9I9_9NE OP C-type lectin domain-containing TRINITY_DN44_c0_g4_i2. tr|A0A5E4Q9I9|A0A5E4Q9I9_9NE protein OS=Leptidea sinapis 34740.HMEL008543-PA p1 OP OX=189913 GN=LSINAPIS_LOCUS6736 PE=4 SV=1 TRINITY_DN514_c0_g1_i2 XP_026501330.1 phenoloxidase XP_026501330.1 34740.HMEL008342-PA .p1 subunit 2-like [Vanessa tameamea] XP_026494663.1 phenoloxidase- TRINITY_DN48030_c0_g1 XP_026494663.1 activating factor 1-like [Vanessa 13037.EHJ65620 _i2.p1 tameamea] tr|Q2F5S1|Q2F5S1_BOMMO Microtubule-associated protein TRINITY_DN1462_c1_g1_i tr|Q2F5S1|Q2F5S1_BOMMO RP/EB family member 3 13037.EHJ77927 7.p1 OS=Bombyx mori OX=7091 PE=2 SV=1 tr|A0A2H1W373|A0A2H1W373_S POFR SFRICE_003398 TRINITY_DN4551_c0_g1_i tr|A0A2H1W373|A0A2H1W373_S OS=Spodoptera frugiperda 13037.EHJ72273 6.p2 POFR OX=7108 GN=SFRICE_003398 PE=4 SV=1 tr|A0A437ASN0|A0A437ASN0_C HISP UBIQUITIN_CONJUGAT_2 TRINITY_DN5161_c0_g1_i tr|A0A437ASN0|A0A437ASN0_C domain-containing protein 13037.EHJ71918 1.p1 HISP OS=Chilo suppressalis OX=168631 GN=evm_014241 PE=4 SV=1 XP_026499334.1 zinc finger TRINITY_DN4589_c0_g1_i XP_026499334.1 protein Xfin-like [Vanessa 34740.HMEL016567-PA 3.p1 tameamea] XP_026490182.1 TRINITY_DN45_c0_g1_i1. XP_026490182.1 phosphatidylinositide phosphatase 13037.EHJ71097 p1 SAC1 [Vanessa tameamea] XP_032513501.1 ecdysone receptor TRINITY_DN4610_c0_g1_i XP_032513501.1 isoform X5 [Danaus plexippus 34740.HMEL012265-PA 16.p1 plexippus] TRINITY_DN4610_c0_g1_i 34740.HMEL012265-PA 4.p3 tr|A0A1C9EGI2|A0A1C9EGI2_BI TRINITY_DN10643_c0_g1 tr|A0A1C9EGI2|A0A1C9EGI2_BI CAN TNF receptor-associated 13037.EHJ68664 _i2.p1 CAN factor 4 OS=Bicyclus anynana OX=110368 PE=4 SV=1 131

XP_026483372.1 mitogen-activated TRINITY_DN4681_c0_g2_i XP_026483372.1 protein kinase-binding protein 1 34740.HMEL015734-PA 2.p1 isoform X5 [Vanessa tameamea] tr|A0A212EH66|A0A212EH66_DA TRINITY_DN46_c0_g1_i4. tr|A0A212EH66|A0A212EH66_DA NPL Putative toll OS=Danaus 13037.EHJ63304 p1 NPL plexippus plexippus OX=278856 GN=KGM_200002 PE=4 SV=1 UniRef90_UPI00052E4986 Ryanodine receptor 1 n=1 TRINITY_DN14933_c0_g1 UniRef90_UPI00052E4986 Tax=Oryctolagus cuniculus 13037.EHJ73734 _i1.p1 TaxID=9986 RepID=UPI00052E4986 tr|A0A212F0J0|A0A212F0J0_DAN PL Ras-related GTP binding protein TRINITY_DN471_c0_g1_i1 tr|A0A212F0J0|A0A212F0J0_DAN OS=Danaus plexippus plexippus 13037.EHJ70641 .p1 PL OX=278856 GN=KGM_207556 PE=4 SV=1 UniRef90_A0A1B0D878 MAP kinase MAP4K4 n=1 TRINITY_DN4758_c1_g1_i UniRef90_A0A1B0D878 Tax=Phlebotomus papatasi 13037.EHJ69742 1.p1 TaxID=29031 RepID=A0A1B0D878_PHLPP TRINITY_DN4771_c0_g2_i 34740.HMEL013337-PA 3.p3 TRINITY_DN4782_c0_g1_i 7091.BGIBMGA002693-

3.p2 TA XP_026496521.1 deformed TRINITY_DN4809_c1_g1_i 7091.BGIBMGA002019- XP_026496521.1 epidermal autoregulatory factor 1 3.p1 TA isoform X2 [Vanessa tameamea] XP_026496512.1 deformed TRINITY_DN4809_c1_g1_i 7091.BGIBMGA002019- XP_026496512.1 epidermal autoregulatory factor 1 5.p1 TA isoform X1 [Vanessa tameamea] tr|A0A1E1WMR8|A0A1E1WMR8_ PECGO Defective in cullin TRINITY_DN4829_c0_g1_i tr|A0A1E1WMR8|A0A1E1WMR8_ neddylation protein (Fragment) 34740.HMEL010069-PA 1.p1 PECGO OS=Pectinophora gossypiella OX=13191 GN=g.17526 PE=4 SV=1 tr|A0A212FBE1|A0A212FBE1_DA NPL Rho-related BTB domain- TRINITY_DN48466_c0_g1 tr|A0A212FBE1|A0A212FBE1_DA containing protein 2 OS=Danaus 13037.EHJ74706 _i1.p1 NPL plexippus plexippus OX=278856 GN=KGM_210262 PE=4 SV=1 tr|A0A212FGP7|A0A212FGP7_DA NPL Mitogen-activated protein TRINITY_DN4957_c0_g1_i tr|A0A212FGP7|A0A212FGP7_DA kinase kinase kinase 4 OS=Danaus 13037.EHJ76732 10.p1 NPL plexippus plexippus OX=278856 GN=KGM_213875 PE=4 SV=1 TRINITY_DN4771_c0_g1_i 13037.EHJ77644 3.p4 tr|Q8WSA4|Q8WSA4_SPOFR Signal transducer and activator of TRINITY_DN1832_c1_g1_i 7091.BGIBMGA001739- tr|Q8WSA4|Q8WSA4_SPOFR transcription OS=Spodoptera 6.p1 TA frugiperda OX=7108 GN=STAT PE=2 SV=1 132

tr|A0A212ELE2|A0A212ELE2_DA NPL WW domain-binding protein 2 TRINITY_DN5051_c0_g1_i tr|A0A212ELE2|A0A212ELE2_DA OS=Danaus plexippus plexippus 13037.EHJ64986 4.p3 NPL OX=278856 GN=KGM_207263 PE=4 SV=1 XP_026490063.1 interferon-related TRINITY_DN4981_c0_g1_i XP_026490063.1 developmental regulator 1 [Vanessa 34740.HMEL015480-PA 1.p1 tameamea] XP_026484156.1 protein argonaute- TRINITY_DN93_c1_g1_i2. 7091.BGIBMGA010406- XP_026484156.1 2-like isoform X2 [Vanessa p1 TA tameamea] TRINITY_DN12089_c1_g1 13037.EHJ76429 _i2.p2 tr|A0A194R3D3|A0A194R3D3_PA TRINITY_DN7907_c0_g1_i tr|A0A194R3D3|A0A194R3D3_PA PMA Ephrin type-B receptor 2 7091.BGIBMGA002079- 10.p1 PMA OS=Papilio machaon OX=76193 TA GN=RR48_09011 PE=3 SV=1 TRINITY_DN19489_c0_g1 XP_026485561.1 protein toll-like 7091.BGIBMGA006244- XP_026485561.1 _i1.p1 isoform X2 [Vanessa tameamea] TA XP_001688415.2 AGAP004138- TRINITY_DN5046_c4_g1_i XP_001688415.2 PA, partial [Anopheles gambiae str. 13037.EHJ75453 1.p1 PEST] tr|A0A212FPQ5|A0A212FPQ5_DA NPL Cap-specific mRNA TRINITY_DN30885_c0_g1 tr|A0A212FPQ5|A0A212FPQ5_DA OS=Danaus plexippus plexippus 13037.EHJ71879 _i1.p1 NPL OX=278856 GN=KGM_207794 PE=4 SV=1 XP_026489152.1 zinc finger TRINITY_DN2056_c0_g1_i XP_026489152.1 protein 1 isoform X1 [Vanessa 13037.EHJ68998 20.p1 tameamea] OWR42743.1 TGF-beta receptor TRINITY_DN655_c0_g1_i7 OWR42743.1 type-1 [Danaus plexippus 34740.HMEL013045-PA .p5 plexippus] tr|A0A2A4JQL3|A0A2A4JQL3_HE LVI PIPK domain-containing TRINITY_DN844_c1_g1_i4 tr|A0A2A4JQL3|A0A2A4JQL3_HE protein OS=Heliothis virescens 13037.EHJ69896 .p1 LVI OX=7102 GN=B5V51_14524 PE=4 SV=1 tr|A0A3S2LQH1|A0A3S2LQH1_C TRINITY_DN5109_c0_g2_i tr|A0A3S2LQH1|A0A3S2LQH1_C HISP Uncharacterized protein 34740.HMEL015036-PA 2.p1 HISP OS=Chilo suppressalis OX=168631 GN=evm_013069 PE=4 SV=1 tr|G3USM6|G3USM6_MELGA TRINITY_DN5118_c0_g1_i TEA domain transcription factor 4 tr|G3USM6|G3USM6_MELGA 13037.EHJ63836 8.p1 OS=Meleagris gallopavo OX=9103 GN=TEAD4 PE=4 SV=1 UniRef90_A0A3Q0J6J2 protein TRINITY_DN5118_c0_g2_i scalloped-like n=1 Tax=Diaphorina UniRef90_A0A3Q0J6J2 13037.EHJ63836 12.p1 citri TaxID=121845 RepID=A0A3Q0J6J2_DIACI XP_026491162.1 transcriptional TRINITY_DN5118_c0_g2_i XP_026491162.1 enhancer factor TEF-1 isoform X1 13037.EHJ63836 9.p1 [Vanessa tameamea] 133

tr|A0A091N723|A0A091N723_APA VI S-phase kinase-associated TRINITY_DN12045_c2_g2 tr|A0A091N723|A0A091N723_APA protein 2 (Fragment) 13037.EHJ69162 _i4.p1 VI OS=Apaloderma vittatum OX=57397 GN=N311_03533 PE=4 SV=1 TRINITY_DN514_c0_g1_i5 GBP70065.1 Phenoloxidase subunit 7091.BGIBMGA013115- GBP70065.1 .p2 2 [Eumeta japonica] TA UniRef90_A0A1E1WH62 Uncharacterized protein (Fragment) TRINITY_DN5150_c1_g1_i UniRef90_A0A1E1WH62 n=1 Tax=Pectinophora gossypiella 13037.EHJ71919 1.p1 TaxID=13191 RepID=A0A1E1WH62_PECGO XP_026487521.1 26S proteasome TRINITY_DN4813_c0_g3_i XP_026487521.1 non-ATPase regulatory subunit 7 34740.HMEL011411-PA 1.p1 [Vanessa tameamea] TRINITY_DN5193_c0_g1_i PCG64196.1 hypothetical protein PCG64196.1 13037.EHJ74014 2.p1 B5V51_11068 [Heliothis virescens] TRINITY_DN5210_c0_g1_i GBP49632.1 Beta-1,3-glucan- GBP49632.1 34740.HMEL003270-PA 10.p1 binding protein [Eumeta japonica] tr|A0A2H1WBV7|A0A2H1WBV7_ SPOFR SFRICE_026408 TRINITY_DN5211_c0_g1_i tr|A0A2H1WBV7|A0A2H1WBV7_ OS=Spodoptera frugiperda 13037.EHJ69744 1.p1 SPOFR OX=7108 GN=SFRICE_026408 PE=3 SV=1 tr|Q6I7P4|Q6I7P4_BOMMO Broad- TRINITY_DN5219_c0_g1_i Complex isoform Z2 OS=Bombyx tr|Q6I7P4|Q6I7P4_BOMMO 13037.EHJ77002 3.p1 mori OX=7091 GN=BmBR-C PE=2 SV=1 KOB68562.1 hypothetical protein TRINITY_DN52498_c1_g1 KOB68562.1 OBRU01_18426, partial 34740.HMEL013671-PA _i1.p1 [Operophtera brumata] TRINITY_DN55681_c0_g1 XP_023288791.1 casein kinase I- XP_023288791.1 7070.TC005606-PA _i1.p1 like, partial [Orussus abietinus] XP_026494532.1 translocator TRINITY_DN2269_c0_g2_i XP_026494532.1 protein isoform X1 [Vanessa 13037.EHJ78740 2.p1 tameamea] TRINITY_DN4251_c0_g1_i GBP30109.1 hypothetical protein GBP30109.1 34740.HMEL011351-PA 12.p1 EVAR_94952_1 [Eumeta japonica] tr|A0A212F846|A0A212F846_DA NPL Scavenger receptor SR-C TRINITY_DN5296_c0_g1_i tr|A0A212F846|A0A212F846_DA protein (Fragment) OS=Danaus 34740.HMEL005258-PA 4.p1 NPL plexippus plexippus OX=278856 GN=KGM_213636A PE=4 SV=1 XP_026488331.1 inward rectifier TRINITY_DN5359_c0_g1_i 7091.BGIBMGA007035- XP_026488331.1 potassium channel 2-like [Vanessa 2.p1 TA tameamea] tr|A0A1E1W8V2|A0A1E1W8V2_P ECGO Uncharacterized protein TRINITY_DN603_c0_g1_i2 tr|A0A1E1W8V2|A0A1E1W8V2_P OS=Pectinophora gossypiella 13037.EHJ63782 8.p1 ECGO OX=13191 GN=g.14560 PE=4 SV=1 tr|A0A2A4JF04|A0A2A4JF04_HEL VI TRAF-type domain-containing TRINITY_DN26906_c0_g1 tr|A0A2A4JF04|A0A2A4JF04_HEL protein OS=Heliothis virescens 34740.HMEL009611-PA _i13.p1 VI OX=7102 GN=B5V51_3459 PE=4 SV=1 134

UniRef90_S4PRM1 Uncharacterized protein (Fragment) TRINITY_DN8812_c1_g2_i UniRef90_S4PRM1 n=1 Tax=Pararge aegeria 34740.HMEL017440-PA 3.p1 TaxID=116150 RepID=S4PRM1_9NEOP tr|A0A5E4QC29|A0A5E4QC29_9N EOP POU domain protein TRINITY_DN5392_c0_g1_i tr|A0A5E4QC29|A0A5E4QC29_9N OS=Leptidea sinapis OX=189913 34740.HMEL011784-PA 2.p1 EOP GN=LSINAPIS_LOCUS7470 PE=3 SV=1 XP_026495201.1 digestive cysteine TRINITY_DN16844_c0_g2 XP_026495201.1 proteinase 2-like [Vanessa 13037.EHJ73161 _i1.p1 tameamea] tr|A0A2P1DMB2|A0A2P1DMB2_S TRINITY_DN544_c0_g1_i8 tr|A0A2P1DMB2|A0A2P1DMB2_S POFR Pasha-PB OS=Spodoptera 34740.HMEL014155-PA .p1 POFR frugiperda OX=7108 PE=2 SV=1 tr|A0A212F957|A0A212F957_DA NPL Histone acetyltransferase TRINITY_DN5460_c0_g2_i tr|A0A212F957|A0A212F957_DA OS=Danaus plexippus plexippus 13037.EHJ73824 6.p1 NPL OX=278856 GN=KGM_213223 PE=3 SV=1 TRINITY_DN11989_c0_g1 XP_026498611.1 cullin-5 [Vanessa XP_026498611.1 34740.HMEL006498-PA _i1.p1 tameamea] TRINITY_DN55206_c0_g1 13037.EHJ69066 _i1.p1 tr|A0A2A4IUU0|A0A2A4IUU0_H TRINITY_DN5520_c0_g1_i tr|A0A2A4IUU0|A0A2A4IUU0_H ELVI Spastin OS=Heliothis 34740.HMEL006972-PA 1.p1 ELVI virescens OX=7102 GN=B5V51_13053 PE=3 SV=1 tr|A0A2A4IUU0|A0A2A4IUU0_H TRINITY_DN5520_c0_g1_i tr|A0A2A4IUU0|A0A2A4IUU0_H ELVI Spastin OS=Heliothis 34740.HMEL006972-PA 12.p1 ELVI virescens OX=7102 GN=B5V51_13053 PE=3 SV=1 tr|A0A2A4IU74|A0A2A4IU74_HE TRINITY_DN5520_c0_g1_i tr|A0A2A4IU74|A0A2A4IU74_HE LVI Spastin OS=Heliothis virescens 34740.HMEL006972-PA 17.p1 LVI OX=7102 GN=B5V51_13053 PE=3 SV=1 TRINITY_DN8018_c0_g1_i XP_026490984.1 hemicentin-1-like XP_026490984.1 13037.EHJ77338 3.p1 [Vanessa tameamea] TRINITY_DN4766_c0_g1_i XP_026501743.1 hemicentin-1 XP_026501743.1 13037.EHJ76866 6.p1 isoform X4 [Vanessa tameamea] TRINITY_DN22647_c0_g1 XP_026491840.1 hemicentin-1-like XP_026491840.1 13037.EHJ71615 _i1.p1 [Vanessa tameamea] TRINITY_DN15528_c1_g1 XP_026493434.1 hemicentin-2-like XP_026493434.1 13037.EHJ71990 _i18.p1 isoform X2 [Vanessa tameamea] TRINITY_DN1400_c0_g1_i XP_026491833.1 hemicentin-1-like XP_026491833.1 34740.HMEL016539-PA 11.p1 [Vanessa tameamea] TRINITY_DN26444_c0_g2 XP_026495222.1 hemicentin-2-like XP_026495222.1 13037.EHJ65737 _i1.p1 isoform X2 [Vanessa tameamea] TRINITY_DN11948_c0_g1 XP_026493388.1 hemicentin-2-like XP_026493388.1 13037.EHJ71990 _i2.p1 [Vanessa tameamea] TRINITY_DN1918_c0_g1_i XP_026491834.1 hemicentin-1-like XP_026491834.1 13037.EHJ72797 1.p1 isoform X1 [Vanessa tameamea] TRINITY_DN109135_c0_g XP_026500237.1 hemicentin-1-like XP_026500237.1 34740.HMEL010770-PA 1_i1.p1 [Vanessa tameamea] 135

TRINITY_DN15050_c0_g1 XP_026484037.1 hemicentin-1-like XP_026484037.1 34740.HMEL004362-PA _i8.p1 [Vanessa tameamea] XP_032517539.1 hemicentin-1 TRINITY_DN14495_c0_g1 XP_032517539.1 isoform X1 [Danaus plexippus 13037.EHJ76524 _i6.p1 plexippus] XP_032517539.1 hemicentin-1 TRINITY_DN14495_c0_g1 XP_032517539.1 isoform X1 [Danaus plexippus 13037.EHJ76524 _i20.p1 plexippus] TRINITY_DN15050_c1_g1 XP_026484037.1 hemicentin-1-like XP_026484037.1 34740.HMEL004362-PA _i1.p1 [Vanessa tameamea] TRINITY_DN1400_c0_g1_i XP_032522846.1 hemicentin-1-like XP_032522846.1 13037.EHJ72797 6.p1 [Danaus plexippus plexippus] TRINITY_DN32230_c0_g1 XP_026495406.1 hemicentin-2-like XP_026495406.1 13037.EHJ71470 _i1.p1 [Vanessa tameamea] TRINITY_DN52237_c0_g1 XP_026491409.1 hemicentin-1-like XP_026491409.1 13037.EHJ78231 _i3.p1 [Vanessa tameamea] XP_026498237.1 LOW QUALITY TRINITY_DN5451_c0_g1_i 7091.BGIBMGA009343- XP_026498237.1 PROTEIN: hemicentin-1-like 11.p1 TA [Vanessa tameamea] UniRef90_A0A2A4ARI1 Hemocyanin_C domain-containing TRINITY_DN99488_c0_g1 protein (Fragment) n=2 UniRef90_A0A2A4ARI1 34740.HMEL015054-PA _i1.p1 Tax=Pseudomonas pelagia TaxID=553151 RepID=A0A2A4ARI1_9PSED tr|A0A5B1L0W0|A0A5B1L0W0_9 ACTN Hemocyanin_C domain- TRINITY_DN99409_c1_g1 tr|A0A5B1L0W0|A0A5B1L0W0_9 containing protein (Fragment) 13037.EHJ65895 _i2.p2 ACTN OS=Nocardioides sp. BN130099 OX=2607660 GN=F0U44_22500 PE=4 SV=1 UniRef90_A0A1E1WDX1 Hemocyanin_M domain-containing TRINITY_DN98270_c3_g1 protein (Fragment) n=2 UniRef90_A0A1E1WDX1 34740.HMEL015049-PA _i1.p1 Tax=Pectinophora gossypiella TaxID=13191 RepID=A0A1E1WDX1_PECGO UniRef90_A0A5B2VU25 Hemocyanin_C domain-containing TRINITY_DN82561_c0_g1 protein (Fragment) n=1 UniRef90_A0A5B2VU25 34740.HMEL015049-PA _i5.p1 Tax=Goodfellowiella sp. AN110305 TaxID=1892852 RepID=A0A5B2VU25_9PSEU tr|A0A5B2VGD2|A0A5B2VGD2_9 PSEU Hemocyanin_C domain- TRINITY_DN58011_c2_g1 tr|A0A5B2VGD2|A0A5B2VGD2_9 containing protein (Fragment) 34740.HMEL009681-PA _i1.p1 PSEU OS=Goodfellowiella sp. AN110305 OX=1892852 GN=F0L68_41555 PE=4 SV=1 tr|A0A5B2UIQ8|A0A5B2UIQ8_9R HIZ Hemocyanin_C domain- TRINITY_DN146677_c4_g tr|A0A5B2UIQ8|A0A5B2UIQ8_9R containing protein (Fragment) 34740.HMEL015049-PA 1_i1.p1 HIZ OS=Salinarimonas sp. BN140002 OX=1638099 GN=F0L46_25680 PE=4 SV=1 136

UniRef90_A0A437BIJ1 Hemocyanin_C domain-containing TRINITY_DN1412_c0_g1_i UniRef90_A0A437BIJ1 protein n=1 Tax=Chilo suppressalis 34740.HMEL008342-PA 1.p1 TaxID=168631 RepID=A0A437BIJ1_CHISP tr|A0A5B2VI38|A0A5B2VI38_9PS EU Hemocyanin_C domain- TRINITY_DN1123_c39_g1 tr|A0A5B2VI38|A0A5B2VI38_9PS containing protein (Fragment) 13037.EHJ65895 _i1.p1 EU OS=Goodfellowiella sp. AN110305 OX=1892852 GN=F0L68_41535 PE=4 SV=1 UniRef90_A0A2A4J253 Hemocyanin_N domain-containing TRINITY_DN26089_c4_g1 protein (Fragment) n=1 UniRef90_A0A2A4J253 13037.EHJ77141 _i1.p1 Tax=Heliothis virescens TaxID=7102 RepID=A0A2A4J253_HELVI tr|A0A5B1L0W0|A0A5B1L0W0_9 ACTN Hemocyanin_C domain- TRINITY_DN33353_c1_g1 tr|A0A5B1L0W0|A0A5B1L0W0_9 containing protein (Fragment) 13037.EHJ65895 _i1.p1 ACTN OS=Nocardioides sp. BN130099 OX=2607660 GN=F0U44_22500 PE=4 SV=1 UniRef90_A0A1E1WRY1 Hemocyanin_M domain-containing TRINITY_DN33342_c0_g1 protein (Fragment) n=1 UniRef90_A0A1E1WRY1 34740.HMEL015049-PA _i6.p1 Tax=Pectinophora gossypiella TaxID=13191 RepID=A0A1E1WRY1_PECGO tr|A0A5E4Q462|A0A5E4Q462_9N EOP Hemocyanin_N domain- TRINITY_DN1305_c1_g1_i tr|A0A5E4Q462|A0A5E4Q462_9N containing protein OS=Leptidea 13037.EHJ65895 39.p3 EOP sinapis OX=189913 GN=LSINAPIS_LOCUS4608 PE=4 SV=1 UniRef90_A0A5B1L1L4 Hemocyanin_C domain-containing TRINITY_DN22683_c3_g1 protein (Fragment) n=1 UniRef90_A0A5B1L1L4 13037.EHJ65895 _i1.p3 Tax=Nocardioides sp. BN130099 TaxID=2607660 RepID=A0A5B1L1L4_9ACTN UniRef90_A0A2A4ANK7 Hemocyanin_M domain-containing TRINITY_DN55125_c0_g1 protein (Fragment) n=1 UniRef90_A0A2A4ANK7 13037.EHJ65899 _i1.p1 Tax=Pseudomonas pelagia TaxID=553151 RepID=A0A2A4ANK7_9PSED UniRef90_A0A5B1L1L4 Hemocyanin_C domain-containing TRINITY_DN98392_c0_g1 protein (Fragment) n=1 UniRef90_A0A5B1L1L4 13037.EHJ65895 _i1.p1 Tax=Nocardioides sp. BN130099 TaxID=2607660 RepID=A0A5B1L1L4_9ACTN UniRef90_A0A1B0DRK6 Hemocyanin_C domain-containing TRINITY_DN32581_c0_g1 UniRef90_A0A1B0DRK6 protein n=1 Tax=Phlebotomus 13037.EHJ77686 _i2.p1 papatasi TaxID=29031 RepID=A0A1B0DRK6_PHLPP 137

UniRef90_Q5MGG3 Hemocyanin_C domain-containing TRINITY_DN33342_c0_g2 protein (Fragment) n=1 UniRef90_Q5MGG3 13037.EHJ65895 _i16.p1 Tax=Lonomia obliqua TaxID=304329 RepID=Q5MGG3_LONON UniRef90_Q5MGG3 Hemocyanin_C domain-containing TRINITY_DN33342_c1_g1 protein (Fragment) n=1 UniRef90_Q5MGG3 34740.HMEL015049-PA _i1.p1 Tax=Lonomia obliqua TaxID=304329 RepID=Q5MGG3_LONON UniRef90_A0A2A4ANK7 Hemocyanin_M domain-containing TRINITY_DN33782_c4_g1 protein (Fragment) n=1 UniRef90_A0A2A4ANK7 34740.HMEL015054-PA _i1.p1 Tax=Pseudomonas pelagia TaxID=553151 RepID=A0A2A4ANK7_9PSED UniRef90_A0A5B1LBI0 Hemocyanin_M domain-containing TRINITY_DN5001_c0_g1_i protein (Fragment) n=1 UniRef90_A0A5B1LBI0 13037.EHJ77141 6.p1 Tax=Nocardioides sp. BN140041 TaxID=2607659 RepID=A0A5B1LBI0_9ACTN tr|A0A5B1L0W0|A0A5B1L0W0_9 ACTN Hemocyanin_C domain- TRINITY_DN52839_c2_g1 tr|A0A5B1L0W0|A0A5B1L0W0_9 containing protein (Fragment) 34740.HMEL015049-PA _i1.p1 ACTN OS=Nocardioides sp. BN130099 OX=2607660 GN=F0U44_22500 PE=4 SV=1 tr|A0A5B1L1L4|A0A5B1L1L4_9A CTN Hemocyanin_C domain- TRINITY_DN52839_c3_g1 tr|A0A5B1L1L4|A0A5B1L1L4_9A containing protein (Fragment) 13037.EHJ65895 _i1.p2 CTN OS=Nocardioides sp. BN130099 OX=2607660 GN=F0U44_22480 PE=4 SV=1

138

Supplementary Table S2. Antimicrobial peptide transcripts found during EnTAP annotation against APD3 database.

Query Sequence Subject Sequence Description

AP01580|TCP (Thrombin-derived C-terminal TRINITY_DN92519_c0_g1_i1.p1 AP01580|TCP Peptide, humans; primates, mammals, animals; Derivatives: VFR17 and HVF18) AP02805|Histone H2A (shrimp, Crustaceans, TRINITY_DN7112_c0_g1_i1.p1 AP02805|Histone arthropods, invertebrates, animals; UCLL1, more AMPs?) AP02037|cgUbiquitin (oyster, TRINITY_DN3681_c1_g1_i12.p2 AP02037|cgUbiquitin mollusca/molluscs/mollusks, invertebrates, animals) AP02037|cgUbiquitin (oyster, TRINITY_DN3681_c0_g1_i7.p1 AP02037|cgUbiquitin mollusca/molluscs/mollusks, invertebrates, animals) AP02341|Galleria mellonella Lysozyme TRINITY_DN1295_c2_g4_i1.p1 AP02341|Galleria (insects, arthropods, invertebrates, animals; UCSS1a) AP02039|LTP110 (lipid transfer protein, plants; TRINITY_DN100586_c0_g1_i1.p1 AP02039|LTP110 4S=S; UCSS1a)

AP00140|SK84 (insects, arthropods, TRINITY_DN16278_c0_g1_i3.p1 AP00140|SK84 invertebrates, animals)

AP03007|BmGlv4 (Gloverin, insects, TRINITY_DN3267_c2_g1_i15.p1 AP03007|BmGlv4 arthropods, invertebrates, animals, UCLL1a)

AP03004|BmGlv1 (Gloverin, insects, TRINITY_DN3188_c4_g1_i1.p1 AP03004|BmGlv1 arthropods, invertebrates, animals, UCLL1a)

AP03106|Splys-i (i-type lysozyme, Crustaceans, TRINITY_DN2992_c0_g1_i1.p1 AP03106|Splys-i arthropods, invertebrates, animals; BBS) AP02037|cgUbiquitin (oyster, TRINITY_DN140197_c0_g1_i1.p1 AP02037|cgUbiquitin mollusca/molluscs/mollusks, invertebrates, animals) AP02810|Histone H3 (mammals, animals; TRINITY_DN1166_c2_g1_i1.p1 AP02810|Histone UCLL1)

AP03004|BmGlv1 (Gloverin, insects, TRINITY_DN1070_c0_g2_i1.p1 AP03004|BmGlv1 arthropods, invertebrates, animals, UCLL1a) AP02668|Lys-49 phospholipase A2 (PLA2), TRINITY_DN33785_c0_g1_i1.p1 AP02668|Lys-49 myotoxin II (snake, reptiles; animals; S=S, UCSS1a) AP02805|Histone H2A (shrimp, Crustaceans, TRINITY_DN3495_c0_g1_i1.p1 AP02805|Histone arthropods, invertebrates, animals; UCLL1, more AMPs?) AP02808|Histone H4 TRINITY_DN20253_c0_g1_i3.p2 AP02808|Histone (mollusca/molluscs/mollusks, invertebrates, animals; UCLL1) 139

AP03106|Splys-i (i-type lysozyme, Crustaceans, TRINITY_DN21074_c0_g2_i3.p1 AP03106|Splys-i arthropods, invertebrates, animals; BBS)

AP00992|potato Snakin-2 (StSN2, 6S=S, TRINITY_DN119765_c0_g1_i1.p1 AP00992|potato UCSS1a; plants)

AP02809|Histone H2B (mammals, animals; TRINITY_DN3495_c1_g1_i1.p1 AP02809|Histone UCLL1)

AP03106|Splys-i (i-type lysozyme, Crustaceans, TRINITY_DN29107_c0_g1_i1.p1 AP03106|Splys-i arthropods, invertebrates, animals; BBS) AP02805|Histone H2A (shrimp, Crustaceans, TRINITY_DN31841_c0_g1_i2.p2 AP02805|Histone arthropods, invertebrates, animals; UCLL1, more AMPs?) AP02341|Galleria mellonella Lysozyme TRINITY_DN3144_c0_g2_i7.p1 AP02341|Galleria (insects, arthropods, invertebrates, animals; UCSS1a) AP02341|Galleria mellonella Lysozyme TRINITY_DN36408_c0_g1_i3.p1 AP02341|Galleria (insects, arthropods, invertebrates, animals; UCSS1a) AP03106|Splys-i (i-type lysozyme, Crustaceans, TRINITY_DN21074_c0_g2_i2.p1 AP03106|Splys-i arthropods, invertebrates, animals; BBS)

140

Supplementary Table S3. Full set of differentially expressed transcripts for each comparison.

Regulation Transcript LFC (log2) FDR (padj) Final Annotation Description

VcB vs. VcS Transcription TRINITY_DN1820_ tr|A0A212FHE0|A0 initiation factor IIF Up-regulated 10.1054 9.1954E-06 c0_g1_i1.p1 A212FHE0_DANPL subunit alpha (Fragment) TRINITY_DN7287_ tr|A0A212ERL5|A0 7.8810 7.7472E-05 Spalt protein c0_g1_i7.p1 A212ERL5_DANPL 40S ribosomal TRINITY_DN5247_ 7.8249 1.0596E-25 GBP72221.1 protein S7 [Eumeta c0_g2_i7.p1 japonica] Retrotrans_gag TRINITY_DN5704_ tr|A0A437B3Z9|A0A 7.3877 4.9572E-02 domain-containing c0_g1_i1.p2 437B3Z9_CHISP protein homeodomain- TRINITY_DN2939_ interacting protein 5.4472 6.2642E-03 XP_026490345 c1_g1_i1.p1 kinase 2-like isoform X2 (HIPK) UDP- TRINITY_DN10026 5.3096 1.3903E-02 XP_026497767.1 glucuronosyltransfer _c0_g1_i8.p1 ase 2A3-like TRINITY_DN2391_ tr|S4PA90|S4PA90_9 Splicing factor 3B 3.5293 4.9572E-02 c0_g2_i12.p2 NEOP subunit (Fragment) pancreatic TRINITY_DN6594_ Down-regulated -1.3682 1.3172E-05 XP_026494764.1 triacylglycerol c0_g1_i1.p1 lipase-like Calponin-homology TRINITY_DN3186_ tr|A0A2A4JQJ7|A0A -2.6343 7.2885E-03 (CH) domain- c0_g1_i16.p1 2A4JQJ7_HELVI containing protein uncharacterized TRINITY_DN8983_ -3.9180 2.5604E-04 XP_028026312.1 protein c0_g1_i3.p1 LOC114240078 10- TRINITY_DN1357_ tr|A0A5E4Q650|A0 -4.1996 8.7771E-03 formyltetrahydrofolat c0_g1_i4.p1 A5E4Q650_9NEOP e dehydrogenase TRINITY_DN3326_ -4.6745 7.2885E-03 XP_023936123.1 Liprin-alpha-1 c0_g1_i14.p1 TRINITY_DN18476 tr|S4NT30|S4NT30_ Elongation factor 1 -5.2876 7.3468E-16 _c0_g2_i1.p1 9NEOP delta TRINITY_DN2269_ translocator protein -7.7652 6.6004E-05 XP_026494533.1 c0_g2_i1.p1 isoform X2 TRINITY_DN3326_ -8.9182 3.3017E-06 XP_023936123.1 Liprin-alpha-1 c0_g1_i10.p1 VcV vs. VcN TRINITY_DN2472_ RNA-binding protein Up-regulated 20.7738 6.7258E-05 XP_026501185.1 c1_g2_i2.p1 45-like uncharacterized TRINITY_DN227_c 10.0770 2.6190E-11 XP_032524538.1 protein 3_g2_i6.p1 LOC116775692 TRINITY_DN910_c tr|A0A3S2N9E0|A0 Uncharacterized 9.2415 2.3985E-09 0_g2_i24.p1 A3S2N9E0_CHISP protein TRINITY_DN4925_ tr|Q9NDV5|Q9NDV 8.9301 1.5937E-08 Alpha-amylase c0_g1_i20.p2 5_BOMMO TRINITY_DN3012_ tr|S4PBQ2|S4PBQ2_ 8.6513 3.9147E-02 Rac1 c2_g1_i4.p1 9NEOP 141

TPR_REGION TRINITY_DN17841 tr|A0A212FAI4|A0A 7.2480 2.8403E-04 domain-containing _c0_g1_i4.p1 212FAI4_DANPL protein TRINITY_DN6828_ tr|A0A212FGC4|A0 Ecdysteroid 22- 6.9950 3.4200E-24 c0_g1_i5.p1 A212FGC4_DANPL kinase tr|A0A4C1W1M8|A0 Integrase catalytic TRINITY_DN6720_ 6.9415 5.7459E-05 A4C1W1M8_EUMV domain-containing c0_g1_i4.p2 A protein collagen alpha- TRINITY_DN121_c 6.7756 3.7124E-03 XP_026486862.1 1(XVIII) chain-like 1_g1_i7.p1 isoform X4 TRINITY_DN8331_ tr|A0A212EPE5|A0A Uncharacterized 6.4686 2.4830E-02 c0_g1_i10.p1 212EPE5_DANPL protein TRINITY_DN3043_ tr|A0A212EJA6|A0A Short spindle protein 6.4062 1.5937E-08 c0_g1_i13.p1 212EJA6_DANPL 4 isoform 3 TRINITY_DN37467 prostamide/prostagla 6.3351 3.4621E-02 XP_026484200.1 _c0_g1_i4.p1 ndin F synthase-like TRINITY_DN222_c tr|H6UFA6|H6UFA6 Amylase, alpha 2A 6.2653 6.8547E-12 0_g1_i23.p1 _9ANUR (Fragment) TRINITY_DN3043_ tr|A0A212EJA6|A0A Short spindle protein 5.5245 6.0428E-06 c0_g1_i27.p1 212EJA6_DANPL 4 isoform 3 TRINITY_DN400_c tr|B7S8P8|B7S8P8_9 Retroelement 5.0440 6.0567E-13 0_g1_i25.p1 HYME polyprotein tr|A0A0N1PHR5|A0 TRINITY_DN18988 4.9296 1.3127E-02 A0N1PHR5_PAPM Collagenase _c0_g2_i4.p1 A Golgi-specific brefeldin A- TRINITY_DN210_c tr|A0A194R5A3|A0 4.7624 4.8212E-02 resistance guanine 0_g1_i10.p1 A194R5A3_PAPMA nucleotide exchange factor 1 TRINITY_DN222_c tr|A0A023UFU0|A0 4.7177 3.3750E-02 Amylase (Fragment) 0_g1_i3.p1 A023UFU0_9TELE LOW QUALITY TRINITY_DN4367_ 4.5415 5.8950E-03 XP_023949648.1 PROTEIN: la-related c0_g1_i27.p1 protein 1, partial TRINITY_DN6559_ 4.4502 4.3713E-02 XP_026489871.1 borealin isoform X2 c0_g1_i3.p1 TRINITY_DN222_c tr|B1NLD4|B1NLD4 4.2998 1.3081E-08 Alpha-amylase 0_g1_i7.p1 _HELAM TRINITY_DN21142 4.0799 1.2278E-22 XP_026490451.1 maltase 1-like _c0_g1_i1.p1 TRINITY_DN8079_ tr|A0A2H1X102|A0 3.7674 7.7287E-06 Lipase c1_g3_i1.p1 A2H1X102_SPOFR MAGUK p55 TRINITY_DN9769_ 3.6334 2.2985E-03 XP_026498015.1 subfamily member 5 c0_g1_i10.p1 isoform X4 TRINITY_DN9769_ MAGUK p55 3.5949 6.4500E-05 GBP10677.1 c0_g1_i11.p1 subfamily member 5 TRINITY_DN14034 3.4341 8.8696E-07 XP_026490452.1 maltase A1-like _c0_g1_i1.p1 TRINITY_DN18815 keratin, type I 3.3558 8.0157E-03 XP_026500577.1 _c0_g1_i1.p1 cytoskeletal 9 venom TRINITY_DN1_c0_ 2.9674 4.3490E-02 XP_026490764.1 carboxylesterase-6- g1_i22.p1 like organic cation TRINITY_DN230_c 2.8029 2.5028E-04 XP_026496283.1 transporter protein- 0_g1_i4.p1 like TRINITY_DN4115_ tr|W5QM97|W5QM9 2.7770 1.0420E-02 Delta11-desaturase c0_g1_i11.p1 7_BICAN uncharacterized TRINITY_DN10786 2.7593 2.5488E-02 XP_023937666.1 protein _c0_g1_i2.p1 LOC112045631 142

Lipase domain- TRINITY_DN265_c UniRef90_A0A0K8S 2.6653 1.2167E-04 containing protein 0_g1_i18.p1 440 (Fragment) TRINITY_DN13636 Acyl-coenzyme A 2.5971 8.5761E-03 KOB75225.1 _c0_g2_i3.p2 oxidase, partial TRINITY_DN6334_ tr|A0A212EIL8|A0A Beta- 2.5378 1.2413E-02 c0_g1_i2.p1 212EIL8_DANPL fructofuranosidase 2 venom TRINITY_DN606_c 2.4061 1.2014E-03 XP_026490764.1 carboxylesterase-6- 0_g1_i10.p1 like Reverse transcriptase TRINITY_DN10428 tr|A0A437AV46|A0 2.3606 2.9319E-04 domain-containing _c0_g2_i2.p1 A437AV46_CHISP protein 1-acyl-sn-glycerol-3- TRINITY_DN9000_ phosphate 2.3537 1.2027E-04 XP_026484415.1 c1_g1_i1.p1 acyltransferase beta- like TRINITY_DN21491 2.3160 4.3713E-02 XP_026490754.1 aquaporin-like _c0_g1_i1.p1 1-acyl-sn-glycerol-3- TRINITY_DN8313_ phosphate 2.2657 5.8411E-07 XP_026484418.1 c0_g1_i7.p1 acyltransferase alpha-like TRINITY_DN4335_ tr|A0A194PL67|A0A 2.2655 7.5820E-03 Protein SON c0_g2_i4.p1 194PL67_PAPXU TRINITY_DN1304_ 2.2507 1.2167E-04 XP_026490183.1 myrosinase 1-like c0_g1_i10.p1 TRINITY_DN9705_ tr|A0A212FF58|A0A Cytochrome P450 2.1350 7.9968E-04 c0_g1_i8.p1 212FF58_DANPL 18a1 TRINITY_DN22704 tr|A0A2H1V294|A0 2.1206 4.2375E-02 SFRICE_025501 _c0_g2_i6.p1 A2H1V294_SPOFR TRINITY_DN3207_ tr|L7X400|L7X400_ Leucine repeat-rich 2.0892 4.2375E-02 c0_g2_i1.p1 HELEA protein uncharacterized TRINITY_DN6782_ 2.0096 9.1796E-03 XP_026495594.1 protein c1_g1_i13.p2 LOC113400287 TRINITY_DN2479_ 1.9656 2.1907E-02 XP_026490184.1 myrosinase 1-like c0_g1_i2.p1 TRINITY_DN9705_ tr|A0A5N6NYF5|A0 Uncharacterized 1.9597 2.8729E-02 c0_g1_i1.p1 A5N6NYF5_9ASTR protein uncharacterized TRINITY_DN4877_ 1.9268 1.9736E-10 XP_022834142.1 protein c1_g1_i2.p2 LOC111361920 tr|A0A2W1B5F8|A0 Reverse transcriptase TRINITY_DN4877_ 1.8881 8.8696E-07 A2W1B5F8_HELA domain-containing c1_g1_i2.p1 M protein TRINITY_DN4057_ tr|A0A194RE10|A0A Organic cation 1.8602 2.5701E-02 c0_g1_i14.p1 194RE10_PAPMA transporter protein uncharacterized TRINITY_DN9710_ 1.8342 3.1749E-03 XP_026494016.1 protein c0_g1_i1.p1 LOC113399157 TRINITY_DN12859 carcinine transporter- 1.7838 5.7459E-05 XP_026485047.1 _c0_g1_i11.p1 like TRINITY_DN12165 tr|L0N6I2|L0N6I2_B 1.7302 3.8808E-05 Cytochrome P450 _c0_g1_i3.p1 OMMO inter-alpha-trypsin TRINITY_DN1331_ 1.6868 1.0460E-06 XP_026484616.1 inhibitor heavy chain c0_g1_i4.p1 H6-like isoform X2 TRINITY_DN1512_ tr|A0A212FJE1|A0A Uncharacterized 1.6705 7.9268E-09 c0_g1_i1.p1 212FJE1_DANPL protein RNA-binding protein TRINITY_DN7873_ 1.5416 1.9399E-02 XP_026494809.1 Musashi homolog c0_g1_i7.p1 Rbp6 isoform X1 143

TRINITY_DN11044 tr|A0A212FHR9|A0 Cathepsin L 1.4822 4.2609E-02 _c0_g1_i2.p1 A212FHR9_DANPL proteinase TRINITY_DN18472 organic cation 1.2796 3.9147E-02 XP_026494449.1 _c0_g1_i1.p1 transporter 1-like lutropin- TRINITY_DN4271_ choriogonadotropic 1.2604 2.5701E-02 XP_026488181.1 c0_g1_i5.p1 hormone receptor- like TRINITY_DN137_c UniRef90_A0A5M6 Uncharacterized 1.2107 2.8871E-02 0_g1_i11.p1 GQU5 protein TRINITY_DN3780_ carboxypeptidase B- 1.1124 1.0082E-03 XP_026494366.1 c0_g1_i4.p1 like TRINITY_DN4210_ tr|A0A194PFD1|A0 Down-regulated -0.9370 2.5038E-02 Cysteine synthase c0_g1_i3.p1 A194PFD1_PAPXU Pheromone-binding TRINITY_DN1119_ tr|A0A194PNB1|A0 -1.0877 4.2609E-02 protein-related c0_g1_i7.p1 A194PNB1_PAPXU protein 2 TRINITY_DN9392_ proton-coupled folate -1.1113 4.2375E-02 XP_026486092.1 c0_g1_i1.p1 transporter-like TRINITY_DN1385_ tr|A0A2H1VGU8|A0 -1.1260 4.9772E-02 SFRICE_005282 c0_g1_i10.p1 A2H1VGU8_SPOFR TRINITY_DN4410_ tr|A0A2H1V482|A0 -1.1684 2.4031E-02 SFRICE_000029 c0_g1_i2.p1 A2H1V482_SPOFR uncharacterized TRINITY_DN1141_ protein -1.2245 2.9724E-02 XP_026494644.1 c0_g2_i2.p1 LOC113399675 isoform X1 TRINITY_DN316_c -1.2286 3.9147E-02 XP_026494383.1 insecticyanin-A-like 0_g1_i6.p1 multidrug resistance- TRINITY_DN54_c6 -1.2664 4.2609E-02 XP_026483243.1 associated protein 1 _g1_i1.p1 isoform X2 UDP- TRINITY_DN96_c0 tr|A0A191T2J5|A0A -1.2801 2.0224E-02 glucuronosyltransfer _g1_i6.p1 191T2J5_SPOEX ase TRINITY_DN273_c -1.2990 2.6640E-02 0_g1_i1.p1 TRINITY_DN10455 lachesin-like isoform -1.3335 4.6509E-02 XP_026485485.1 _c0_g1_i58.p1 X2 TRINITY_DN25405 L-asparaginase-like -1.3651 6.1131E-04 XP_026494340.1 _c1_g1_i1.p1 isoform X1 DNA_MISMATCH_ TRINITY_DN6968_ tr|A0A5E4R485|A0A -1.3686 1.4432E-03 REPAIR_2 domain- c0_g1_i2.p1 5E4R485_9NEOP containing protein TRINITY_DN11282 tr|F1AAN7|F1AAN7 -1.4301 1.0420E-02 Yellow-f4 _c0_g1_i2.p1 _HELME Reticulocyte-binding TRINITY_DN478_c tr|S4PSI4|S4PSI4_9 -1.4628 4.2473E-02 protein 2-like protein 0_g1_i1.p1 NEOP (Fragment) adenylate kinase TRINITY_DN15926 -1.6208 4.2775E-03 XP_030028369.1 isoenzyme 1 isoform _c0_g1_i5.p1 X2 calponin homology domain-containing TRINITY_DN692_c -1.6508 4.4557E-02 XP_026494193.1 protein 0_g1_i19.p1 DDB_G0272472 isoform X3 retinol TRINITY_DN2511_ -1.8554 2.7030E-02 XP_026496871.1 dehydrogenase 14- c0_g1_i10.p1 like Mitochondrial TRINITY_DN1771_ tr|A0A212ESQ8|A0 phosphoenolpyruvate -1.8570 2.0884E-02 c0_g1_i1.p1 A212ESQ8_DANPL carboxykinase isoform 1 144

mediator of RNA TRINITY_DN5639_ polymerase II -1.9579 2.8655E-04 XP_026484186.1 c1_g1_i1.p1 transcription subunit 15-like vascular endothelial TRINITY_DN192_c -1.9652 3.2880E-04 XP_026491088.1 growth factor 3_g2_i2.p1 receptor 1 ribose-phosphate TRINITY_DN3807_ -1.9985 2.0719E-05 XP_026493541.1 pyrophosphokinase c0_g2_i1.p1 2-like TRINITY_DN3268_ tr|A0A2A4IY47|A0A Uncharacterized -2.0032 4.9770E-02 c0_g1_i21.p1 2A4IY47_HELVI protein (Fragment) TRINITY_DN2145_ tr|A0A2A4JJF7|A0A ENTH domain- -2.0054 4.1376E-04 c1_g1_i1.p1 2A4JJF7_HELVI containing protein TRINITY_DN6914_ tr|A0A212FEF6|A0A Zinc finger protein -2.0276 4.4417E-03 c0_g2_i3.p1 212FEF6_DANPL GLIS2 TRINITY_DN7176_ tr|A0A212F7Q2|A0A Uncharacterized -2.0525 3.7694E-02 c0_g1_i11.p2 212F7Q2_DANPL protein Reverse transcriptase TRINITY_DN7540_ tr|A0A2A4J3A4|A0 Ty1/copia-type -2.1594 3.4958E-03 c0_g1_i1.p2 A2A4J3A4_HELVI domain-containing protein discoidin domain- TRINITY_DN5632_ -2.2358 4.2473E-02 XP_026500512.1 containing receptor c4_g1_i1.p1 2-like isoform X2 UDP- TRINITY_DN2422_ -2.3466 1.5918E-03 XP_026483600.1 glucuronosyltransfer c0_g1_i1.p1 ase 2B19-like Retrovirus-related TRINITY_DN7540_ sp|P10978|POLX_T -2.6295 2.8655E-04 Pol polyprotein from c0_g1_i1.p1 OBAC transposon TNT 1-94 TRINITY_DN23649 tr|A0A286SBT4|A0 -2.6668 4.2375E-02 Calreticulin _c0_g2_i1.p1 A286SBT4_CNAME 10- TRINITY_DN1357_ tr|A0A5E4Q650|A0 -2.9752 6.5795E-03 formyltetrahydrofolat c0_g1_i4.p1 A5E4Q650_9NEOP e dehydrogenase TRINITY_DN5248_ cytochrome P450 -3.1036 1.6653E-02 XP_026488879.1 c0_g1_i1.p1 6B2-like uncharacterized TRINITY_DN8983_ -3.1489 3.3115E-04 XP_028026312.1 protein c0_g1_i3.p1 LOC114240078 Translocation TRINITY_DN4346_ tr|S4PMB1|S4PMB1 -3.2327 5.7556E-03 associated membrane c0_g1_i1.p1 _9NEOP protein phospholipid transfer TRINITY_DN592_c -3.6176 3.1866E-02 XP_023938342.1 protein C2CD2L 0_g1_i29.p1 isoform X5 TRINITY_DN3328_ RNA-binding protein -3.6238 5.8950E-03 XP_026497077.1 c0_g1_i1.p1 FUS-like uncharacterized TRINITY_DN62849 -3.9285 3.4773E-02 XP_026488774.1 protein _c0_g1_i6.p1 LOC113395395 calcium-binding TRINITY_DN1302_ mitochondrial carrier -4.0046 2.9144E-03 XP_026489654.1 c0_g2_i7.p1 protein SCaMC-2 isoform X1 TRINITY_DN2942_ UniRef90_A0A5B1L Uncharacterized -4.0117 1.9566E-03 c40_g1_i1.p1 136 protein (Fragment) TRINITY_DN168_c protein bric-a-brac 2- -4.3830 1.0752E-03 XP_026497334.1 1_g1_i1.p1 like isoform X2 TRINITY_DN3771_ tr|W6JHY5|W6JHY5 -4.4273 1.5431E-02 Homothorax c0_g2_i6.p1 _BOMMO TRINITY_DN1421_ tr|A0A2A4K7W9|A0 Uncharacterized -5.7916 3.4200E-24 c0_g1_i1.p1 A2A4K7W9_HELVI protein 145

uncharacterized TRINITY_DN604_c -6.5783 2.0884E-02 XP_026497692.1 protein 0_g1_i23.p1 LOC113401839 TRINITY_DN7287_ tr|A0A212ERL5|A0 -7.7206 1.2068E-04 Spalt protein c0_g1_i7.p1 A212ERL5_DANPL tyrosine-protein TRINITY_DN2033_ -7.7937 1.2326E-05 XP_026486949.1 phosphatase Lar c0_g1_i6.p1 isoform X3 TRINITY_DN4264_ larval cuticle protein -9.2477 2.8982E-04 XP_026492845.1 c0_g1_i2.p1 LCP-30-like VcV vs. VcB TRINITY_DN3019_ tr|A0A212FB13|A0A Ufm1-conjugating Up-regulated 9.3572 5.2607E-04 c0_g1_i1.p1 212FB13_DANPL enzyme 1 TRINITY_DN3326_ 8.9176 1.4776E-06 XP_023936123.1 liprin-alpha-1 c0_g1_i10.p1 TRINITY_DN3451_ tr|A0A194Q9D0|A0 8.1574 7.4780E-07 Drebrin-like protein c0_g1_i11.p1 A194Q9D0_PAPXU TPR_REGION TRINITY_DN17841 tr|A0A212FAI4|A0A 7.4725 4.9402E-04 domain-containing _c0_g1_i4.p1 212FAI4_DANPL protein G-protein coupled TRINITY_DN10216 7.0760 3.5088E-02 XP_026499602.1 receptor Mth2-like _c0_g2_i2.p1 isoform X1 tr|A0A2H1WM69|A TRINITY_DN7438_ 6.6163 3.0791E-02 0A2H1WM69_SPOF SFRICE_013324 c0_g1_i1.p1 R uncharacterized TRINITY_DN227_c 5.6282 1.9770E-03 XP_032524538.1 protein 3_g2_i6.p1 LOC116775692 tr|A0A0N1PHR5|A0 TRINITY_DN18988 5.4326 1.1767E-02 A0N1PHR5_PAPM Collagenase _c0_g2_i4.p1 A TRINITY_DN18476 tr|S4NT30|S4NT30_ Elongation factor 1 5.0282 5.5099E-16 _c0_g2_i1.p1 9NEOP delta TRINITY_DN3326_ 4.6724 5.9106E-03 XP_023936123.1 liprin-alpha-1 c0_g1_i14.p1 TRINITY_DN18742 tr|A0A2A4JIG9|A0A Uncharacterized 4.1207 2.6763E-03 _c0_g1_i4.p1 2A4JIG9_HELVI protein scavenger receptor TRINITY_DN105_c 2.1243 2.9835E-06 XP_026483476.1 class B member 1- 1_g4_i1.p1 like oxygen-dependent TRINITY_DN2336_ coproporphyrinogen- 1.7650 1.4455E-02 XP_026488669.1 c0_g1_i1.p2 III oxidase isoform X2 TRINITY_DN1123_ facilitated trehalose Down-regulated -1.4196 2.4325E-02 XP_026498763.1 c0_g1_i7.p3 transporter Tret1-like organic cation TRINITY_DN1696_ -1.6848 1.5024E-02 XP_026496367.1 transporter protein c0_g1_i8.p1 isoform X1 TRINITY_DN636_c tr|H9JDF0|H9JDF0_ Carboxylic ester -1.7038 3.8643E-02 0_g2_i5.p1 BOMMO hydrolase Phenylalanyl-tRNA TRINITY_DN10795 tr|A0A212F320|A0A -1.8159 1.8897E-03 synthetase beta _c0_g1_i12.p1 212F320_DANPL subunit TRINITY_DN2145_ tr|A0A2A4JJF7|A0A ENTH domain- -1.9669 4.9031E-02 c1_g1_i1.p1 2A4JJF7_HELVI containing protein putative UDP- TRINITY_DN2545_ -2.2634 2.7528E-02 XP_023943058.1 glucuronosyltransfer c0_g1_i29.p1 ase ugt-47 UDP- TRINITY_DN350_c tr|G9LPS9|G9LPS9_ -2.3250 3.8489E-02 glucuronosyltransfer 0_g1_i10.p1 BOMMO ase 146

TRINITY_DN275_c trypsin, alkaline C- -2.3826 2.7251E-02 XP_026497535.1 0_g1_i19.p1 like TRINITY_DN9812_ UniRef90_A0A3M3 Uncharacterized -2.4193 3.8643E-02 c0_g2_i1.p1 EUH8 protein oxygen-dependent TRINITY_DN2336_ coproporphyrinogen- -2.4916 1.9770E-03 XP_026488668.1 c0_g1_i12.p2 III oxidase isoform X1 UDP- TRINITY_DN2422_ -2.6859 4.7469E-02 XP_026483600.1 glucuronosyltransfer c0_g1_i1.p1 ase 2B19-like tr|A0A2H4RMW1|A CRAL-TRIO TRINITY_DN7977_ -2.6989 3.0245E-02 0A2H4RMW1_BIC domain-containing c0_g1_i2.p1 AN protein TRINITY_DN11110 tr|A0A212F388|A0A -3.0645 4.6615E-02 Bombyrin _c1_g1_i6.p1 212F388_DANPL TRINITY_DN314_c -3.0991 4.6615E-02 XP_028164442.1 esterase FE4-like 1_g1_i1.p1 Integrase catalytic TRINITY_DN1369_ tr|A0A0N1IHU1|A0 -3.2813 6.0227E-03 domain-containing c1_g1_i3.p1 A0N1IHU1_PAPMA protein TRINITY_DN5228_ serine protease -3.2825 4.6094E-02 AAA29331.1 c3_g3_i1.p1 inhibitor, partial TRINITY_DN11432 tr|A0A194PS51|A0A Reelin domain- -3.4862 3.0547E-02 _c0_g1_i1.p2 194PS51_PAPXU containing protein TRINITY_DN10653 alaserpin-like -3.5554 1.9802E-02 XP_026485746.1 _c0_g1_i7.p1 isoform X2 uncharacterized TRINITY_DN2449_ protein -4.6985 1.5958E-03 XP_026486129.1 c0_g1_i2.p1 LOC113393458 isoform X1 TRINITY_DN5632_ tr|Q2V0Q1|Q2V0Q1 GTP cyclohydrolase -5.1787 2.2412E-02 c1_g1_i14.p1 _PAPXU I isoform A TRINITY_DN3188_ -5.2143 1.5958E-03 XP_026497749.1 gloverin-like c4_g1_i1.p1 TRINITY_DN1315_ tr|A0A3S2TTM1|A0 Uncharacterized -5.8920 3.2828E-02 c0_g1_i3.p1 A3S2TTM1_CHISP protein TRINITY_DN1421_ tr|A0A2A4K7W9|A0 Uncharacterized -6.0795 1.7916E-16 c0_g1_i1.p1 A2A4K7W9_HELVI protein TRINITY_DN2834_ tenascin-like isoform -6.1126 9.3070E-05 XP_026491494.1 c0_g3_i3.p1 X2 TRINITY_DN1070_ -6.4267 3.7290E-04 XP_026497729.1 gloverin-like c0_g2_i1.p1 TRINITY_DN1047_ tr|A0A212FKX9|A0 -6.5303 3.3590E-05 Attacin c0_g1_i3.p1 A212FKX9_DANPL TRINITY_DN820_c protein FAM102A -6.7669 2.8280E-02 XP_021188082.1 0_g2_i11.p1 isoform X2 LOW QUALITY TRINITY_DN14145 -6.9957 1.7409E-02 XP_028159201.1 PROTEIN: lebocin- _c0_g1_i2.p1 4-like TRINITY_DN6998_ tr|A0A212EPE5|A0A Uncharacterized -7.4131 1.5991E-03 c0_g1_i14.p1 212EPE5_DANPL protein TRINITY_DN13230 tr|A0A437B944|A0A Uncharacterized -7.5593 6.0227E-03 9_c0_g1_i1.p1 437B944_CHISP protein inducible TRINITY_DN1710_ -7.6191 6.7112E-03 XP_026485721.1 metalloproteinase c0_g1_i17.p2 inhibitor protein-like TRINITY_DN7287_ tr|A0A212ERL5|A0 -7.8163 9.3070E-05 Spalt protein c0_g1_i7.p1 A212ERL5_DANPL TRINITY_DN3267_ -7.8658 6.0227E-03 XP_026497720.1 gloverin-like c2_g1_i15.p1 147

larval/pupal rigid TRINITY_DN5121_ -7.9195 3.7396E-06 XP_026488169.1 cuticle protein 66- c0_g2_i3.p1 like tr|A0A315V6M7|A0 Aconitase_C TRINITY_DN12384 -8.8472 3.6314E-03 A315V6M7_GAMA domain-containing _c0_g1_i2.p1 F protein (Fragment) VcN vs. VcS TRINITY_DN642_c Up-regulated 9.3639 2.5290E-06 0_g1_i2.p2 TRINITY_DN3043_ tr|A0A212EJA6|A0A Short spindle protein 9.0873 5.3860E-09 c0_g1_i40.p1 212EJA6_DANPL 4 isoform 3 TRINITY_DN3043_ tr|A0A212EJA6|A0A Short spindle protein 8.7276 7.0753E-03 c0_g1_i37.p1 212EJA6_DANPL 4 isoform 3 TRINITY_DN7287_ tr|A0A212ERL5|A0 7.7966 2.3528E-04 Spalt protein c0_g1_i7.p1 A212ERL5_DANPL TRINITY_DN3043_ tr|A0A212EJA6|A0A Short spindle protein 7.3312 3.9594E-05 c0_g1_i2.p1 212EJA6_DANPL 4 isoform 3 TRINITY_DN5247_ 40S ribosomal 7.3082 6.7521E-25 GBP72221.1 c0_g2_i7.p1 protein S7 methionine-rich TRINITY_DN52570 6.4887 1.5698E-07 AAB14955.1 storage protein, _c0_g1_i2.p1 partial TRINITY_DN2942_ UniRef90_A0A5B1L Uncharacterized 6.2959 1.9347E-03 c40_g1_i1.p1 136 protein (Fragment) phospholipid transfer TRINITY_DN592_c 5.5059 1.1231E-04 XP_023938342.1 protein C2CD2L 0_g1_i29.p1 isoform X5 TRINITY_DN29559 4.4187 4.3663E-02 _c0_g1_i1.p1 uncharacterized TRINITY_DN13448 4.4069 1.9634E-04 XP_026497202.1 protein _c0_g4_i1.p1 LOC113401483 TRINITY_DN1464_ tr|A0A437BH42|A0 Uncharacterized 3.4168 7.0751E-05 c2_g1_i11.p1 A437BH42_CHISP protein (Fragment) TRINITY_DN1234_ alpha-1- 2.9920 1.6886E-03 XP_023936578.1 c1_g1_i10.p1 macroglobulin-like GATA zinc finger TRINITY_DN3017_ domain-containing 2.7810 1.9662E-02 XP_026493082.1 c0_g1_i2.p1 protein 14-like isoform X1 TRINITY_DN1856_ tr|A0A194PJV5|A0A 1.9993 1.3324E-04 Anoctamin c0_g1_i8.p1 194PJV5_PAPXU TRINITY_DN671_c tr|S4PHD4|S4PHD4_ 1.8870 3.9353E-02 Cysteine proteinase-1 0_g1_i18.p1 9NEOP ribose-phosphate TRINITY_DN3807_ 1.5689 1.1576E-02 XP_026493541.1 pyrophosphokinase c0_g2_i1.p1 2-like Mitochondrial TRINITY_DN1771_ tr|A0A212ESQ8|A0 phosphoenolpyruvate 1.1639 6.5639E-04 c0_g1_i1.p1 A212ESQ8_DANPL carboxykinase isoform 1 Voltage-dependent TRINITY_DN15830 tr|Q90X82|Q90X82_ Down-regulated -1.0828 2.2931E-03 anion channel _c0_g1_i11.p3 GILMI (Fragment) TRINITY_DN1177_ tr|A0A194Q4R1|A0 Arrestin domain- -1.5236 1.9347E-03 c1_g1_i2.p1 A194Q4R1_PAPXU containing protein 3 uncharacterized TRINITY_DN9710_ -1.5532 2.3959E-02 XP_026494016.1 protein c0_g1_i1.p1 LOC113399157 TRINITY_DN2382_ tr|A0A291P0Y3|A0A Carboxylic ester -2.2151 2.6554E-02 c0_g1_i1.p1 291P0Y3_HELAU hydrolase TRINITY_DN8079_ tr|A0A2H1X102|A0 -2.6249 2.3959E-02 Lipase c1_g3_i1.p1 A2H1X102_SPOFR 148

neurogenic locus TRINITY_DN4508_ -2.7247 4.5487E-07 XP_023953386.1 notch homolog c0_g2_i3.p1 protein 1 isoform X2 TRINITY_DN11432 tr|A0A194PS51|A0A Reelin domain- -3.1319 5.1089E-07 _c0_g1_i1.p2 194PS51_PAPXU containing protein 10- TRINITY_DN1357_ tr|A0A5E4Q650|A0 -3.7185 4.8847E-03 formyltetrahydrofolat c0_g1_i4.p1 A5E4Q650_9NEOP e dehydrogenase TRINITY_DN11507 tr|A0A371IIE7|A0A3 Uncharacterized -4.0861 4.7033E-02 3_c0_g1_i1.p1 71IIE7_MUCPR protein (Fragment) TRINITY_DN2668_ tr|A0A212ESK0|A0 -4.7804 2.5452E-07 Hemolin c1_g1_i1.p1 A212ESK0_DANPL TRINITY_DN400_c tr|B7S8P8|B7S8P8_9 Retroelement -4.9172 4.5660E-02 0_g1_i25.p1 HYME polyprotein TRINITY_DN3043_ tr|A0A212EJA6|A0A Short spindle protein -5.1384 1.9347E-03 c0_g1_i13.p1 212EJA6_DANPL 4 isoform 3 TRINITY_DN3043_ tr|A0A212EJA6|A0A Short spindle protein -5.3857 8.0836E-06 c0_g1_i27.p1 212EJA6_DANPL 4 isoform 3 TRINITY_DN19475 tr|T1EAZ7|T1EAZ7_ Putative secreted -5.7923 1.3575E-04 _c0_g2_i3.p1 ANOAQ protein (Fragment) tr|A0A4C1WP33|A0 TRINITY_DN27979 Transposon Tf2-11 -6.1246 1.2214E-02 A4C1WP33_EUMV _c0_g1_i10.p2 polyprotein A TRINITY_DN8331_ tr|A0A212EPE5|A0A Uncharacterized -6.4606 4.7928E-02 c0_g1_i10.p1 212EPE5_DANPL protein TRINITY_DN37467 prostamide/prostagla -7.1005 2.9338E-03 XP_026484200.1 _c0_g1_i4.p1 ndin F synthase-like GATA zinc finger TRINITY_DN8296_ domain-containing -7.4080 1.3181E-14 XP_026493082.1 c0_g1_i1.p1 protein 14-like isoform X1 TRINITY_DN6828_ tr|A0A212FGC4|A0 Ecdysteroid 22- -8.1432 2.4795E-29 c0_g1_i5.p1 A212FGC4_DANPL kinase uncharacterized TRINITY_DN27979 -8.4964 6.8205E-03 XP_026499898.1 protein _c0_g2_i4.p1 LOC113403532 TRINITY_DN3012_ tr|S4PBQ2|S4PBQ2_ -8.7796 4.0349E-12 Rac1 c2_g1_i4.p1 9NEOP TRINITY_DN7508_ tr|A0A5E4PQ04|A0 LRRCT domain- -10.4003 1.2243E-06 c0_g1_i14.p1 A5E4PQ04_9NEOP containing protein TRINITY_DN200_c -10.4630 5.1106E-07 UniRef90_B4QHC7 GD25582 1_g1_i1.p2 acyl-CoA-binding TRINITY_DN2600 -11.6398 1.2409E-17 XP_026492429.1 domain-containing _c0_g1_i3.p1 protein 6-like *Red stars signify transcripts identified as having an immune related function

149

LIST OF SUPPLEMENTARY FIGURES

Supplementary Figure S1. Bubble plot of all enriched GO terms for the bacterially- injected versus saline-injected comparison. GO terms are plotted on the y-axis and the percentage of hits along the x-axis. The size of each bubble represents the count for each GO id and the color represents the corresponding p-value (< 0.05). BP = Biological Process, CC = Cellular Component, MF = Molecular Function ...... 149

Supplementary Figure S2. Bubble plot of all enriched GO terms for the virally-infected versus non-infected comparison. GO terms are plotted on the y-axis and the percentage of hits along the x-axis. The size of each bubble represents the count for each GO id and the color represents the corresponding p-value (< 0.05). BP = Biological Process, CC = Cellular Component, MF = Molecular Function ...... 150

Supplementary Figure S3. Bubble plot of all enriched GO terms for the virally-infected versus bacterially-injected comparison. GO terms are plotted on the y-axis and the percentage of hits along the x-axis. The size of each bubble represents the count for each GO id and the color represents the corresponding p-value (< 0.05). BP = Biological Process, CC = Cellular Component, MF = Molecular Function ...... 151

Supplementary Figure S4. Bubble plot of all enriched GO terms for the non-infected versus saline-injected comparison. GO terms are plotted on the y-axis and the percentage of hits along the x-axis. The size of each bubble represents the count for each GO id and the color represents the corresponding p-value (< 0.05). BP = Biological Process, CC = Cellular Component, MF = Molecular Function ...... 152

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