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AP50167PU-N Polyclonal Antibody to Alpha- 2B / AMY2B (N-term) - Aff - Purified

Alternate names: 1, 4-alpha-D-glucan glucanohydrolase 2B, Carcinoid alpha-amylase Quantity: 0.4 ml Concentration: lot specific Background: are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The has a cluster of several amylase that are expressed at high levels in either salivary gland or pancreas. This encodes an amylase isoenzyme produced by the pancreas. Uniprot ID: P19961 NCBI: NP_066188 GeneID: 280 Host / Isotype: Rabbit / Ig Immunogen: KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human AMY2B Format: State: Liquid purified Ig fraction Purification: Affinity chromatography on Protein A Buffer System: PBS containing 0.09% (W/V) sodium azide as preservative Applications: ELISA: 1/1000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100. Immunofluorescence: 1/10 - 1/50. Flow Cytometry: 1/10 - 1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody reacts to Alpha-amylase 2B. Species Reactivity: Tested: Human. Add. Information: Molecular Weight: 57710 Da Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Baltova, S.D. Folia Med (Plovdiv) 47(1):37-41(2005) 2. Fortna, A., et al. PLoS Biol. 2 (7), E207 (2004) 3. Koyama, I., et al. Clin. Chim. Acta 309(1):73-83(2001) 4. Aughsteen, A.A. Saudi Med J 22(5):410-415(2001) 5. Omichi, K., et al. Biochim. Biophys. Acta 1203(2):224-229(1993)

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 1 / 3 AP50167PU-N: Polyclonal Antibody to Alpha-amylase 2B / AMY2B (N-term) - Aff - Purified

Pictures: AMY2B antibody (N-term) (Cat. #AP50167PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMY2B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of AMY2B Antibody (N-term) (Cat#AP50167PU-N) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

AMY2B Antibody (N-term) (Cat. #AP50167PU-N) western blot analysis in CEM cell line lysates (35µg/lane).This demonstrates the AMY2B antibody detected the AMY2B protein (arrow).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 2 / 3 AP50167PU-N: Polyclonal Antibody to Alpha-amylase 2B / AMY2B (N-term) - Aff - Purified

AMY2B Antibody (N-term) (Cat. #AP50167PU-N) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti- rabbit secondary antibodies were used for the analysis.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 3 / 3

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