Experience.

FLOW CYTOMETRY

Molecular Laboratory Network, Inc. (MPLN) has been on the forefront of laboratory medicine since its inception. Utilizing state-of-the-art instrumentation, our technologists provide a full spectrum of services to aid in the diagnosis and monitoring of hematologic disorders. Experience. MPLN.

Innovative…Comprehensive… Clinical Utility Consultative Immunophenotypic analysis by multiparameter flow Designed to provide accurate, reliable and clinically cytometry is a powerful tool in the evaluation of relevant data in a timely fashion. hematolymphoid neoplasia. At MPLN, interdisciplinary testing on a single sample is reviewed closely to • Streamlined ordering/reporting to simplify reflex testing enhance the flow cytometry data; this includes clinical, morphologic, molecular, and genetic findings. • One source for ancillary testing • Rapid turnaround time By analyzing the expression of cell surface and cytoplasmic markers, abnormal cells can be identified, • Post analytic review for quality assurance enumerated and characterized with respect to cell • Individual case management solving clinical problems lineage and maturation. The sensitivity of the technology and the ability to process and analyze large amounts of Multiparameter data quickly allows flow cytometry to be used to: Immunophenotyping by 1. Characterize/classify Flow Cytometry • Hematolymphoid neoplasia Management of patient testing requires a rational • Myelodysplastic/myeloproliferative processes approach to identify the most effective therapy • Acute leukemias and control treatment cost. Our Flow Cytometry • B-cell testing includes: • Plasma cell disorders • T-cell/NK cell neoplasms • Comprehensive Lymphoma and/or Leukemia immunophenotyping • Rare/unusual processes (mast cell disorders, etc.) • Lymphocyte screen (B, T, NK cells) 2. Determine proliferative rates of lymphomas • Monoclonal gammopathy (B-cell, plasma cell) 3. Determine treatment efficacy/monitor for residual • Residual disease (specified) • PNH • Other special markers

250 East Broadway, Maryville, TN 37804 • main 865.380.9746 • toll free 800.932.2943 • MPLNet.com Experience.

FLOW CYTOMETRY

Case Study Unusual Acute Promyelocytic Leukemia, reflexed for FISH analysis; t(15;17) positive. Histogram 1 Histogram 2 Histogram 3 • Histogram 1: Promyelocytes isolated by SSC v CD45 • Histogram 2: CD34 negative/CD117 positive promyelocytes (expected)

• Histogram 3: CD11b negative (expected), HLA DR CD 45 PC 7 CD117 PC5 CD11b PE positive (unusual). The specimen was reflexed for PML/RARA fusion by fluorescence in situ Log side scatter CD34 FITC HLA-DR ECD hybridization and was positive. Comprehensive Panels In-Depth Report Samples are received and processed by experienced The report is designed to convey clinically relevant laboratory personnel per established, validated protocols information in an easy to read format. The findings and guidelines. Screening panels for all address sample quality, sample cellularity, and sample types are designed to reliably detect abnormal quantitation and characterization of all major cell populations. The comprehensive panel includes markers lines. When present, abnormal populations are for adequate characterization of all cell lines. Additional clearly described, including cell type, percentage of testing, as indicated, may be used to further and fully involvement, cell size, and immunophenotypic features. characterize any abnormal cells detected. The interpretation is written to address clinically relevant issues based on the provided information, Unique Approach to Data Analysis previous studies at MPLN, and dialogue with the Many laboratories use basic analysis techniques and submitting physician. Ancillary testing is discussed provide pathologists with pre-printed histograms; with the client, when indicated, to maximize clinical this approach, although common, is less than optimal. information from the submitted sample. At MPLN, the raw (list mode) data is independently analyzed by a hematopathologist with experience Communication with Hematopathologist at a level not routinely offered by other laboratories. A hematopathologist is available to discuss any After sample processing and appropriate quality questions related to samples. review with respect to sample, reagent and instrument Additionally, we welcome suggestions to help us parameters, the list mode data is analyzed by the improve our service, and we actively seek suggestions hematopathologist. Previous patient results, history to help achieve our goal of providing accurate, reliable and clinical information are incorporated in the analysis, and clinically relevant data in a timely fashion. If you and possible ancillary testing (FISH, cytogenetics, want to discuss any of the above topics or specific molecular, etc.) is considered. results, feel free to call.

One Source Contact Us Coordinating laboratory tests and results within one For more information, visit us online at facility, MPLN provides a single source for flow cytometry, www.MPLNet.com or by phone at 800.932.2943. anatomic pathology, , cytogenetics, and molecular testing. Using one source for your laboratory testing provides simpler logistics for ordering, molecular reflex testing, reporting, billing, and patient management.

(Rev 12/2018)

250 East Broadway, Maryville, TN 37804 • main 865.380.9746 • toll free 800.932.2943 • MPLNet.com