Polyclonal Antibody to Keratin-34 (KRT34) - Serum

OriGene Technologies, Inc. OriGene Technologies GmbH

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BP5085 Polyclonal Antibody to Keratin-34 (KRT34) - Serum

Alternate names: HHA4, HKA4, Hair keratin, KRT-34, KRTHA4, Keratin, Keratin34, type I Ha4, type I cuticular Ha4 Quantity: 0.1 ml Background: KRT34 is a type I hair keratin. Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins are acidic proteins and are usually found as a heterotetramer with two molecules of a type II keratin. Uniprot ID: O76011 NCBI: NP_066293.2 GeneID: 100653049 Host: Guinea Pig Immunogen: Synthetic peptide of human acidic hair (trichocytic) keratin K34 (formerly also designated keratin hHa4; GPC GTS QKG CCN), coupled to KLH. Format: State: Liquid serum contains 0.09% sodium azide Applications: Immunoblotting: 1 : 2,000, ECL method. Immunohistochemistry on frozen sections: 1/200; 1h at RT. NOTE: For enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100 (in PBS) for 1 - 5 min prior to first antibody incubation step. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: The antiserum stains specifically human hair keratin K34 expressed in the upper hair cortex cells. Reacts with human and mouse. Species: Human, Mouse. Other species not tested. Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. General Readings: 1. Langbein L, Rogers M, Winter H, Prätzel S, Beckhaus U, Rackwitz HR, Schweizer J: The catalog of human hair keratins: I. Expression of nine type I members in the hair follicle. J Biol Chem 274, 19874-19893 (1999) 2. Langbein L, Spring H, Rogers MA, Praetzel S, Schweizer J. Hair keratins and hair follicle-specific epithelial keratins. Methods Cell Biol. 2004;78:413-51. PubMed PMID: 15646627.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130513 1 / 1