For Research Use Only

Beta 2 Polyclonal antibody www.ptglab.com Catalog Number:10171-1-AP Featured Product 11 Publications

Catalog Number: GenBank Accession Number: Purification Method: Basic Information 10171-1-AP BC007427 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 350 μg/ml by 409 WB 1:1000-1:4000 Nanodrop and 273 μg/ml by Bradford Full Name: IP 0.5-4.0 ug for IP and 1:500-1:2000 method using BSA as the standard; arrestin, beta 2 for WB Source: IHC 1:50-1:500 Calculated MW: IF 1:20-1:200 Rabbit 46 kDa Isotype: Observed MW: IgG 50 kDa Immunogen Catalog Number: AG0227

Applications Tested Applications: Positive Controls: IF, IHC, IP, WB,ELISA WB : RAW 264.7 cells, rat brain tissue, PC-12 cells, Cited Applications: RAW264.7 IF, IHC, WB IP : RAW 264.7 cells, Species Specificity: IHC : human tonsil tissue, human malignant human, mouse, rat melanoma tissue, human colon cancer tissue, human Cited Species: spleen tissue human, mouse, Xenopus IF : mouse Retina tissue, Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Beta Arrestin 2 is a member of arrestin/beta-arrestin family, which are expressed at high levels in the Background Information central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.

Notable Publications Author Pubmed ID Journal Application Tian-Tian Wei 32948825 Acta Pharmacol Sin IF Jing-Yu Lin 34480896 J Biol Chem WB Huiyan Xu 29116339 J Mol Med (Berl) WB,IHC

Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data

IF result of anti-ARRB2 (10171-1-AP) in Mouse RAW264.7 cells were subjected to SDS PAGE Immunohistochemical analysis of paraffin- Retina by Dr. Seongjin Seo. IS (Inner segment) followed by western blot with 10171-1-AP (Beta embedded human tonsil tissue slide using 10171- Stain. Arrestin 2 antibody) at dilution of 1:2000 incubated 1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 at room temperature for 1.5 hours. (under 10x lens).

Immunohistochemical analysis of paraffin- IP Result of anti-Beta Arrestin 2 (IP:10171-1-AP, embedded human tonsil tissue slide using 10171- 4ug; Detection:10171-1-AP 1:1000) with RAW 264.7 1-AP (Beta Arrestin 2 antibody) at dilution of 1:200 cells lysate 4000ug. (under 40x lens).