DATA SHEET

Goat anti-CHMP5 Antibody

Item Number dAP-0633

Target Molecule Principle Name: CHMP5; Official Symbol: CHMP5; All Names and Symbols: CHMP5; modifying 5 ; HGNC:26942; C9orf83; CGI-34; HSPC177; PNAS-2; SNF7DC2; SNF7 domain containing 2; 9 open reading frame 83; Vps60; Accession Number (s): NP_057494.3; Human ID(s): 51510; Non-Human GeneID(s):

Immunogen NKDGVLVDEFGLPQ, is from C Terminus

Applications Pep ELISA, WB

Species Tested: Human

Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. Peptide ELISA Peptide ELISA: antibody detection limit dilution 1 to 8000.

Western Blot Western Blot: Approx 28kDa band observed in lysates of K562 (calculated MW of 24.5kDa according to NP_057494.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ward et al, J Biol Chem. 2005 Mar 18;2

IHC

Reference Reference(s): Ward DM, Vaughn MB, Shiflett SL, White PL, Pollock AL, Hill J, Schnegelberger R, Sundquist WI, Kaplan J. The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells. J Biol Chem. 2005 Mar 18;280(11):10548-55. Epub 2005 Jan 11. .PMID: 15644320 ->

Optimal dilutions should be determined by each laboratory for each application. The listed dilutions are for recommendation only and the final condi- tions should be optimized by the ender users! This product is sold for Research Use Only

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