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UW Medicine Anatomic Pathology Specimen Collection Manual

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UW Medicine Anatomic Pathology Specimen Collection Manual UW Medicine Anatomic Pathology Specimen Collection Manual UW Medicine Pathology Mission Statement The Division of Anatomic Pathology is committed to excellence in the diagnosis of human disease. Our department and team members are committed to: • Delivering timely and accurate diagnoses that support outstanding patient care and inform effective and appropriate treatment options for patients. • Providing fellows, residents and medical school students with exceptional education and experience, creating leaders within pathology and within healthcare. • Striving to provide educational services within UWMC that enhance the clinical expertise of our medical colleagues and promote the advancement of medical knowledge through basic and clinical research. • Employing compassion throughout all diagnostic services. • Focusing on patient and employee safety and on quality. UW Medicine Pathology Vision Statement The Division of Anatomic Pathology is a recognized leader in quality diagnostic services, state of the art research, comprehensive teaching and is engaged in continually evolving methods to offer excellent and meaningful diagnoses. Contents General Information ................................................................................................................1-1 1.1 Laboratory Contact Information ................................................................................................ 1-1 1.2 Specimen Labeling and Submission ........................................................................................... 1-2 1.3 Specimen Rejection .................................................................................................................... 1-3 Gynecological Cytology Service ................................................................................................2-1 2.1 Gynecological ThinPrep Pap Test ............................................................................................... 2-1 Non-Gyn Cytology Services ......................................................................................................3-1 3.1 Bladder Washing ........................................................................................................................ 3-1 3.2 Body Cavity Fluid (peritoneal wash, pleural ascites, pericardial, etc.) ...................................... 3-2 3.3 Bronchial Brushing ..................................................................................................................... 3-3 3.4 Bronchial Washing ..................................................................................................................... 3-4 3.5 Cerebrospinal Fluid (CSF) ........................................................................................................... 3-5 3.6 Fine Needle Aspiration (FNA) ..................................................................................................... 3-6 3.7 Miscellaneous Aspiration (Cysts, Joints, Etc) ............................................................................. 3-7 3.8 Miscellaneous Brushing (Renal, Gastric, Common Bile Duct, Endocervical Cytobrush, Etc) ..... 3-8 3.9 Sputum ....................................................................................................................................... 3-9 3.10 Sputum for PCP ........................................................................................................................ 3-10 3.11 Thyroid Aspirate ....................................................................................................................... 3-11 3.12 Urine – Voided ......................................................................................................................... 3-12 3.13 Urine – Catheterized ................................................................................................................ 3-13 Molecular Diagnostics Services ................................................................................................4-1 4.1 Human Papilloma Virus (GYN in ThinPrep Pap Test Media) ...................................................... 4-1 4.1.1 GYN in SurePath Media .............................................................................................................. 4-2 4.1.2 GYN in Qiagen Specimen Transport Media (STM) ..................................................................... 4-3 4.1.3 Anal in Qiagen Specimen Transport Media (STM) ..................................................................... 4-4 4.1.4 Anal in ThinPrep Media ............................................................................................................. 4-5 Histology Services ....................................................................................................................5-1 5.1 Frozen Sections UWMC-MT ....................................................................................................... 5-1 5.2 Frozen Sections UWMC-NW ...................................................................................................... 5-2 5.3 Frozen Sections HMC ................................................................................................................. 5-3 5.4 Gross Examination ..................................................................................................................... 5-4 ii 5.5 Bone Marrow Biopsy Specimens ............................................................................................... 5-5 5.6 Renal Biopsy Specimens ............................................................................................................. 5-6 5.7 Surgical Tissue Specimens .......................................................................................................... 5-7 5.8 Tissue Biopsy Specimens ............................................................................................................ 5-8 Neuropathology Services .........................................................................................................6-1 6.1 Brain/Spine Biopsy Specimens ................................................................................................... 6-1 6.2 Brain Tumor Specimen for Fluorescent In Situ Hybridization (FISH) ......................................... 6-2 6.3 Muscle Specimens ...................................................................................................................... 6-3 6.4 Nerve Specimens........................................................................................................................ 6-4 6.5 Ocular Biopsy Specimens ........................................................................................................... 6-5 Immunofluorescence ...............................................................................................................7-1 7.1 Direct Immunofluorescence ...................................................................................................... 7-1 7.1.1 Oral Biopsy Specimens ............................................................................................................... 7-1 7.1.2 Skin Specimens ........................................................................................................................... 7-2 7.2 In-Direct Immunofluorescence .................................................................................................. 7-3 7.2.1 Serum Specimen ......................................................................................................................... 7-3 Cytogenetics Services...............................................................................................................8-1 8.1 Amniotic Fluid ............................................................................................................................ 8-1 8.2 Bone Marrow / Oncology Blood ................................................................................................ 8-2 8.3 Chorionic Villi ............................................................................................................................. 8-3 8.4 DNA Samples .............................................................................................................................. 8-4 8.5 Peripheral Blood ........................................................................................................................ 8-5 8.6 Solid Tissue (including Products of Conception, Skin Biopsies, Stillbirths) ................................ 8-6 8.7 Urine........................................................................................................................................... 8-7 8.8 Biliary Duct Brushing .................................................................................................................. 8-8 Additional Testing Services ......................................................................................................9-1 9.1 Electron Microscopy Services: ................................................................................................... 9-1 9.2 DNA Flow Cytometry .................................................................................................................. 9-2 Outside Slides/Block Review Services ..................................................................................... 10-1 10.1 Slide Reviews (UW Medicine Patients) .................................................................................... 10-1 Slide Consults (External consultation with UW
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