Nutritional and Antinutritional Properties of Carissa Carandas and Cordia Dichotoma, Two Medicinally Important Wild Edible Fruits of Odisha

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Nutritional and Antinutritional Properties of Carissa Carandas and Cordia Dichotoma, Two Medicinally Important Wild Edible Fruits of Odisha J. Basic. Appl. Sci. Res. , 7(7)1-12, 2017 ISSN 2090-4304 Journal of Basic and Applied © 2017, TextRoad Publication Scientific Research www.textroad.com Nutritional and Antinutritional Properties of Carissa carandas and Cordia dichotoma, two Medicinally Important Wild Edible Fruits of Odisha Pragyan Aparichita Patra, Uday Chand Basak * Seed Bank and Seed Biology Division, Regional Plant Resource Centre, (R & D Institute of Forest and Environment Department), Bhubaneswar-751015, Odisha, India Received: April 1, 2017 Accepted: June 29, 2017 ABSTRACT The aim of this study was to estimate and compare major nutritional and antinutritional properties of Carissa carandas and Cordia dichotoma fruits collected from various agro-climatic forest zones of Odisha. Nutritional results showed that Cordia dichotoma from Malkangiri region contain maximum amount of moisture (80.24±2.14 % fwt.), acidity (0.31±0.01 % fwt.), total protein (2.26±0.19 % fwt.), total carbohydrate (31.6±0.91% fwt.), total sugar (14.11±0.39 % fwt.), reducing sugar (7.28±0.39 % fwt.) and non-reducing sugar (6.83±0.39 % fwt.) whereas in Carissa carandas , highest moisture (90.59±3.07 %fwt.) and titrable acidity (0.53 ± 0.06 %fwt.) was seen in Khorda region. Maximum total carbohydrate (13.75%fwt.), total sugar (8.46% fwt.) and reducing sugar (4.62% fwt.) found in Malkangiri region. From antinutritional analysis, results showed that Cordia dichotoma contain least amount of saponin (0.193±0.008 % dry wt.) and phytate (1.25±0.13 mg/g dry wt.) from Malkangiri region whereas least oxalate (3.00±0.33 mg/g dry wt.) and tannin (0.045±0.001 TAE g/g dry wt.) content was observed in Carissa carandas from Kalahandi region. Thus, the impact of different geographic locations on nutrients and antinutrients composition in the studied fruits was also evident from the analysis. KEYWORDS: Wild edible fruits, Nutritional, Antinutritional, Regional variation. 1. INTRODUCTION Forests play a large and indispensable role in improving food security for rural and mainly tribal aboriginals. Since immemorial, wild edible fruits of Odisha are being utilized by various tribal communities like Santal, Kolha, Bhuyan, Munda, Lodha, Bathudi, Bhumij, Gond, Mundari, Mankidia, Mahali, Sounti, Saora, Juang, Banjara, Bhottada, Bhujia, Binjhal, Dal, Kandha, Mirdha, Paroja, Shabar etc. in various traditional forms as food, fodder, condiment, medicine, beverages etc. [1,2,3,4]. Wild edible fruits provide nutritional and nutraceutical opportunities. The variable climate and diversified topography of the state contribute to its rich and varied flora [4]. Carissa carandas and Cordia dichotoma are distributed in different regions of Odisha, composition of its nutritional and antinutritional properties vary greatly depending on their geographic locations and various other factors. This is mainly due to the prevailing favourable climate for growth of wild edible fruits, availability of abundant irrigation water. Climate, water balance and life form have more shaped the patterns in fruit nutrients as well as antinutrients. According to the reports, Carissa carandas and Cordia dichotoma fruits were found medicinally important illustrated in Table 1. Malnutrition is a major health burden in developing nation and recognition of these wild edible fruits which are nutritionally potent can provide dietary supplement to the human body [5,6]. Antinutritional factors interfere with the bioavailability of nutrients required by the body; it may be wise to analyze the antinutritional contents according to their nutritional properties so that the users can be informed to make right decision to consume [7,8]. Wild edible fruits have escaped recognition and scientific enquiry. Hence, sustainable utilization of these potential species can add value addition to poorest household and can be further used in pharmaceutical industries. Considering the multifarious usefulness of these wild fruits, the present study was undertaken for generating baseline data on the nutraceutical potential of these wild fruits as influenced by various agro-climatic regions of Odisha to facilitate its potential use in preparation of dietary supplement and processed food products or functional foods. 2. MATERIALS AND METHODS 2.1 Sample collection Fresh ripened fruits were collected from 3 different agro-climatic forest zones of Odisha i.e. (i)South Eastern Ghat represented by ‘Malkangiri’ district (17° 52.426 ″ N and 081° 31.675 ″ E), (ii) Western Undulating Corresponding Author: Uday Chand Basak, Seed Bank and Seed Biology Division, Regional Plant Resource Centre, (R & D Institute of Forest and Environment Department), Bhubaneswar-751015, Odisha, India. Email id: [email protected] ; Contact No-9437481352, Fax-0674-2550274 1 Patra and Basak 2017 zone represented by ‘Kalahandi’ district (19° 26 ′ 821 ″ N, 082° 48 ′ 882 ″ E) and (iii) East and South-Eastern Coastal Plain represented by ‘Khorda’ district (20° 29 ′ 91 ″ N, 85° 80 ′ 22 ″ E) during the month of May-July, 2016. Collection of fruit samples for both the species has been done by random sampling from their respective region. Immediately after collection, fruits were brought to the laboratory. The fruit samples were authenticated with the help of Ref. Books e.g. Flora of Odisha [9], Wild Edible Fruit Plants of Eastern India [10, 11] and Lesser Known Crops and Wild Edible Fruits of Uttarakhand [12]. It was also compared with authentic herbarium sheets belonging to the Herbarium section of Regional Plant Resource Centre, Bhubaneswar. Table 1. Phenology and medicinal uses of Carissa carandas and Cordia dichotoma fruits Sl. No. Name of fruit species Flowering & Fruiting season Medicinal Uses 1. Carissa carandas L. Flowering Fruits help in antiviral [13] March- April Antiscorbutic, antidiabetic and Fruiting antimicrobial [14]. Good source of vitamin- July- October c and pectin. Mature fruit are rich in pectin [15]. Family: Apocynaceae Local name: Karanda koli 2. Cordia dichotoma Forst. Flowering Fruits used in wound healing activity [16], March- April anthelmintic [17] and antidiabetic [18]. It Fruiting has gastroprotective and antiulcer activity July- October [19]. Fruit gum contains anti-inflammatory activity and acts as emulsifier [20]. Family: Ehretiaceae Local name: Guala koli 2.2 Methodology for nutritional evaluation 2.2.1 Estimation of Moisture content The collected fruit samples were determined for moisture content [21]. An empty dish was dried and weighed. 3 gm of fresh fruit added to the dish. The sample was spread uniformly and kept in hot air oven for 3 hours at 105ºC. After drying, the sample was kept for cooling and the dish was reweighed. Moisture (%) = (W1-W2) x 100 W1 Where: W1= weight (g) of sample before drying W2= weight (g) of sample after drying 2.2.2 Estimation of Acidity content Acidity content of the sample was determined by using Phenolphthalein indicator and sodium hydroxide [22]. Fresh sample (0.5gm of deseeded pulp) was weighed and 10ml of distilled water was added to it. The sample was homogenized with the help of mortar pestle and kept in a beaker. 5ml of sample extract was taken in a conical flask and 2 drops of Phenolphthalein indicator was added and titrated against 0.1 N NaOH solution. A faint pink colour appeared and that is the end point. The burette reading was noted and calculated as per details given below. Titrable Acidity was expressed as Tartaric Acid = ml of alkali ×Normality of Alkali X 7.5 Weight. of Samples in Grams 2.2.3 Estimation of Total Protein content Protein was estimated using Folin Phenol reagent [23]. Fruit samples (1 gm) was homogenized with cold Tris–Glycine buffer solution at a pH of 7.9. It was then centrifuged at 10000 rpm for 20 minutes at 4ºC (Eppendorf cold centrifuge). 2 J. Basic. Appl. Sci. Res. , 7(7)1-12, 2017 After centrifugation, the pellet was discarded and to the supernatant equal volume of pre-chilled TCA solution was added and stored overnight to precipitate out all the debris at 4ºC. Next day the supernatant and TCA mixture was again centrifuged at 10, 000 rpm for 15 minutes at 4ºC. After centrifugation the supernatant was discarded and the tight, creamy-white coloured pellet was collected. It was then treated with 1 ml of 0.1 N NaOH solutions and kept as protein stock solution. For quantification, 50µl of sample was taken and adjusted to 1ml by adding distilled water. Then to that sample, 4 ml of (0.1N NaOH, 2% Na 2CO 3,1% CuSO 4, 2% Sodium Potassium tartrate) solution was added and allowed to stand in room temperature for 10 minutes. 0.5 ml of Folin phenol was added and it was incubated in room temperature for 30 minutes. After 30 minutes, absorbance was measured at 750 nm in spectrophotometer (Spekol 2000, Analytik Jena Make). Total protein content was calculated against the BSA standard. 2.2.4 Estimation of Total carbohydrate content Total Carbohydrate content was estimated by Anthrone method [24]. The fruit extract was prepared by taking 100 mg of fruit sample and hydrolyzing the fruit sample with 2.5N HCl for 3 hours in water bath at 100 °C. After heating, it was neutralized with sodium carbonate. The sample extract was then centrifuged at 3000 rpm for 20 min (Eppendorf cold Centrifuge, 5430 R) and the supernatant was stored at 4 °C. To 0.5 ml of test sample, 4 ml of Anthrone reagent was added to test tubes and heated for eight minutes at boiling temperature in water bath. It was cooled rapidly before measuring OD at 630 nm in spectrophotometer (Spekol 2000, Analytik Jena Make). A standard graph was made by plotting different concentrations of the D-Glucose standard on the X-axis versus corresponding absorbance on the Y- axis. From the graph the amount of carbohydrate present in the fruit sample was calculated. 2.2.5 Estimation of Total Sugar content Total sugar value of the sample was evaluated as described [22].
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