Polyclonal Antibody to UTY (C-Term) - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP54497PU-N Polyclonal Antibody to UTY (C-term) - Aff - Purified

Alternate names: Histone demethylase UTY, Ubiquitously-transcribed TPR protein on the Y chromosome, Ubiquitously-transcribed Y chromosome tetratricopeptide repeat protein Quantity: 0.4 ml Concentration: lot specific Background: UTY encodes a protein containing tetratricopeptide repeats which are thought to be involved in protein-protein interactions. This protein is a minor histocompatibility antigen which may induce graft rejection of male stem cell grafts. Uniprot ID: O14607 NCBI: NP_009056 GeneID: 7404 Host / Isotype: Rabbit / Ig Immunogen: KLH conjugated synthetic peptide between 1254~1284 amino acids from the C- terminal region of human UTY Format: State: LIquid Ig fraction Purification: Protein A column followed by peptide affinity purification Buffer System: PBS with 0.09% (W/V) sodium azide Applications: ELISA: 1:1;000. Western blot: 1:100~500. Flow cytometry: 1:10~50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody detects UTY (C-term). Species Reactivity: Tested: Human Add. Information: Molecular Weight: 149548 Da Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Russo,P., et.al., Arterioscler. Thromb. Vasc. Biol. 28 (8), 1569-1574 (2008). 2. Gerrard,D.T. et.al., Mol. Biol. Evol. 22 (6), 1423-1432 (2005).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 1 / 2 AP54497PU-N: Polyclonal Antibody to UTY (C-term) - Aff - Purified

Pictures: Western blot analysis of UTY Antibody (C- term) (AP54497PU-N) in mouse brain tissue lysates (35 µg/lane). UTY (arrow) was detected using the purified Pab.

UTY Antibody (C-term) (AP54497PU-N) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC- conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 2 / 2