DOCSLIB.ORG

Antioxidant Activity of Pluchea Indica Less. Extract After in Vitro Digestion and Absorption by Caco-2 Cell Line

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Antioxidant Activity of Pluchea Indica Less. Extract After in Vitro Digestion and Absorption by Caco-2 Cell Line ANTIOXIDANT ACTIVITY OF PLUCHEA INDICA LESS. EXTRACT AFTER IN VITRO DIGESTION AND ABSORPTION BY CACO-2 CELL LINE KRISADAPORN POLSIRI A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE MASTER DEGREE OF SCIENCE IN BIOLOGICAL SCIENCE FACULTY OF SCIENCE BURAPHA UNIVERSITY AUGUST 2015 COPYRIGHT OF BURAPHA UNIVERSITY ACKNOWLEDGEMENT In the success of this thesis, I would like to express my sincere gratitude and deep appreciation to my principal advisor, Dr. Chatchawin Petchlert for support, attention, motivation, technical assistance, helpful suggestion and comment, encouragements and support throughout my study. I would like to thank Dr. Nattida Chotechuang as an external examiner from Department of Food Technology, Faculty of Science, Chulalongkorn University and Dr. Salil Chanroj as an examiner from Faculty of Science, for all of their guidance, valuable advice through this thesis. Great appreciation is also given to Biological Science Graduate Program, Department of Biochemistry for laboratory facilities. I would like to thanks Professor Dr. Nateetip Krishnamra from Department of Physiology, Faculty of Science, Mahidol University for providing the Caco-2 cells in this research. I would like to thank Assistant Professor Dr. Klaokwan Srisook for technical assistance in Cell culture. I would like to thank Dr. Somchart Maenpuen for helpful suggestion and comment, encouragements and support in this research. I would like to thank Dr. Panata Wanichwatanadecha for helpful suggestion in this research. I also would like to thank Burapha University and Faculty of Science for the financial support financially supported. I would like to thank research grant 2014 from Burapha University (supporting grant from National Research Council). Finally, a great respect is brought to my parents and my family for their love, takecaring, attention, encouragement, guidance and supporting throughout my study. I specially thank to all lecturers of Department of Biochemistry and all of my friends for their kindness, help, suggestion, encouragement, solidarity, and togetherness. Krisadaporn Polsiri THE RELEVANCE OF THE RESEARCH WORK TO THAILAND The main purposes of this research are to know the basic knowledge of the antioxidant activity of Pluchea indica Less. tea extracts both before and after in vitro digestion including absorption by Caco-2 cell lines. This research project provided supporting data for the use of the plants and its compound for the well-being of people with a help of science. This research project provided support the utilization of this tea. It may be used to consume as a healthy food. Providing the scientific evidence to justify the use of P. indica leaves in traditional medicine. iv 53910187: MAJOR: BIOLOGICAL SCIENCE; M.Sc. (BIOLOGICAL SCIENCE) KEYWORDS: PLUCHEA INDICA LESS./ IN VITRO DIGESTION/ DPPH/ REDUCING POWER/ TOTAL PHENOLIC CONTENT/ TOTAL FLAVONOID CONTENT/ CACO-2 CELL MONOLAYERS KRISADAPORN POLSIRI: ANTIOXIDANT ACTIVITY OF PLUCHEA INDICA LESS. EXTRACT AFTER IN VITRO DIGESTION AND ABSORPTION BY CACO-2 CELL LINE. ADVISORY COMMITTEE: CHATCHAWIN PETCHLERT, Ph.D. 94 P. 2015. Pluchea indica Less. is an evergreen large shrub found abundantly in salt marshes and mangrove swamps in India, Bangladesh, Myanmar, China, the Philippines, Malaysia, Thailand and Australia. P. indica Less. is a plant with anti- inflammatory and antioxidant medicinal properties. However, antioxidant activity of P. indica tea extract (PITE) after in vitro digestion have not ever been studied. This study aimed to investigate antioxidant activity of PITE both pre and post-in vitro digestion including absorption by Caco-2 cells. Such activities were performed using DPPH radical scavenging assay, reducing power, total phenolic and total flavonoid contents. Pre-digestion of PITE at 0.1 mg/ml showed a strong scavenging activity on DPPH by 84.77%. When IC50 was considered to indicate the potential of this activity, the value in the intestinal digestion had the lowest value with 0.0187±0.008 mg/ml when compared to all phases. DPPH radical scavenging activity of PITE has been studied across Caco-2 cell monolayers. The results showed that PITE might be accessible via the epithelial cells that resulted in the reduction of percent scavenging activity in the basolateral side of transwell. However, P. indica tea was still effective for this activity. Reducing power pre-digestion of PITE was 137.82±0.07 mg QE/g extract whereas this power after oral and intestinal digestion was not detectable. In addition, total phenolic and total flavonoid contents of PITE in pre-digestion showed high amount were 196.56±0.02 and 243.75±0.01 mg QE/g extract, respectively. Total phenolic content of PITE in post-digestion decreased nearly seven folds. Total flavonoid content of PITE was attenuated in oral and gastric digestion, but it increased after intestinal digestion (286.58±0.05 mg QE/g extract). Then total phenolic and total v flavonoid content of PITE has been studied across Caco-2 monolayers. PITE could pass through the Caco-2 cells to basolateral side. However, total phenolic and total flavonoid contents were significantly decreased in the apical and basolateral sides. In conclusion, P. indica Less. tea may be considered as an alternative healthcare to consumers. CONTENTS Page ABSTRACT........................................................................................................ iv CONTENTS........................................................................................................ vi LIST OF TABLES............................................................................................... x LIST OF FIGURES............................................................................................. xi CHAPTER 1. INTRODUCTION..................................................................................... 1 1.1 Objectives...................................................................................... 2 1.2 Contribution to knowledge............................................................ 3 1.3 Scope of the study.......................................................................... 3 2. LITERATURE REVIEWS........................................................................ 4 2.1 Free radicals.................................................................................... 4 2.1.1 Types of free radicals............................................................. 4 2.1.2 Formation of free radicals...................................................... 7 2.1.3 Steps involving free radical generation……………………... 8 2.1.3.1 Initiation..................................................................... 8 2.1.3.2 Propagation................................................................. 9 2.1.3.3 Termination................................................................. 9 2.1.4 Molecular damage induced by free radicals............................ 11 2.1.4.1 Lipids........................................................................... 11 2.1.4.2 Carbohydrates............................................................. 11 2.1.4.3 DNA............................................................................ 11 2.1.4.4 Proteins....................................................................... 12 2.2 Antioxidant..................................................................................... 12 2.2.1 Types of antioxidants.............................................................. 13 2.2.1.1 Enzymatic antioxidants................................................ 13 2.2.1.2 Nonenzymatic antioxidants.......................................... 14 2.3 Determination of antioxidant activity............................................. 20 2.3.1 2, 2-diphenyl- 1-picryl-hydrazyl (DPPH) scavenging assay... 20 vii CONTENTS (CONTINUED) Chapter Page 2.3.2 Reducing power..................................................................... 21 2.3.3 Total phenolic content............................................................ 21 2.3.4 Total flavonoid content........................................................... 22 2.4 Human digestive system................................................................. 22 2.4.1 Component of the digestive system........................................ 23 2.4.1.1 The Mouth ………………........................................... 23 2.4.1.2 The Esophagus.............................................................. 24 2.4.1.3 Stomach........................................................................ 24 2.4.1.4 Small Intestine.............................................................. 25 2.4.1.5 Large Intestine.............................................................. 26 2.4.1.6 Pancreas, Liver, and Gall bladder................................. 27 2.4.1.7 The Rectum................................................................... 28 2.4.1.8 The Anus........................................................................ 28 2.5 Absorption ………………………………………………………... 28 2.6 Caco-2 cell monolayer model.......................................................... 29 2.7 Background of Pluchea indica Less................................................ 31 2.7.1 Properties ……………………................................................ 32 2.7.2 Parts used................................................................................. 32 2.7.3 Medicinal use........................................................................... 32 2.7.4 Studies....................................................................................
Load more

Recommended publications
  • "National List of Vascular Plant Species That Occur in Wetlands: 1996 National Summary."
    Intro 1996 National List of Vascular Plant Species That Occur in Wetlands The Fish and Wildlife Service has prepared a National List of Vascular Plant Species That Occur in Wetlands: 1996 National Summary (1996 National List). The 1996 National List is a draft revision of the National List of Plant Species That Occur in Wetlands: 1988 National Summary (Reed 1988) (1988 National List). The 1996 National List is provided to encourage additional public review and comments on the draft regional wetland indicator assignments. The 1996 National List reflects a significant amount of new information that has become available since 1988 on the wetland affinity of vascular plants. This new information has resulted from the extensive use of the 1988 National List in the field by individuals involved in wetland and other resource inventories, wetland identification and delineation, and wetland research. Interim Regional Interagency Review Panel (Regional Panel) changes in indicator status as well as additions and deletions to the 1988 National List were documented in Regional supplements. The National List was originally developed as an appendix to the Classification of Wetlands and Deepwater Habitats of the United States (Cowardin et al.1979) to aid in the consistent application of this classification system for wetlands in the field.. The 1996 National List also was developed to aid in determining the presence of hydrophytic vegetation in the Clean Water Act Section 404 wetland regulatory program and in the implementation of the swampbuster provisions of the Food Security Act. While not required by law or regulation, the Fish and Wildlife Service is making the 1996 National List available for review and comment.
    [Show full text]
  • Pluchea Indica Less) LEAVES EXTRACT AS ANTIOXIDANT and ANTI WARMED OVER FLAVOR (WOF) of DUCK MEAT
    POTENCY OF BELUNTAS (Pluchea indica Less) LEAVES EXTRACT AS ANTIOXIDANT AND ANTI WARMED OVER FLAVOR (WOF) OF DUCK MEAT Paini Sri Widyawati1, Tarsisius Dwi Budianta, Fenny Anggraeni Kusuma, Evelyn Livia Wijaya, Dian Ivana Yaunatan, Ribka Stefanie Wongso Faculty of Agricultural Technology, Widya Mandala Catholic University of Surabaya Jl. Dinoyo 42‐44 Surabaya, 60265, Indonesia Correspondent author: : [email protected] Abstract Beluntas (Pluchea indica Less) is a herb plant used as a traditional medicine or eaten in fresh form. There are phytochemical compounds such as essential oils, flavonoids, phenolics, tannins, saponins, phenols hydroquinone, and cardiac glycosides compounds of beluntas leaves that cause it having potential as anti‐ oxidant. Difference of solvent polarity can cause concentration and composition of phytochemical com‐ pounds in extract differed, therefore this study was conducted to determine the potential for beluntas leaves extracts (water, methanol, ethanol, ethyl acetate, and hexane) and fractions (water, ethyl acetate, and n‐butanol) as an antioxidant and antiwarmed over flavor (WOF) in duck meat during storage. The results showed that the methanol extract of the beluntas leaves (EMB) of the most potential as a source of antioxidants because the concentration and composition of phytochemical compounds, total phenols and total flavonoids than the water, ethanol, ethyl acetate, and hexane extracts. Furthermore EMB was frac‐ tionated by difference of solvent polarity (ethyl acetate, water, n‐butanol). Test showed that EMB antioxi‐ dant capacity and its fractions had the difference in the ability of antioxidant compounds in the EMB and each fraction in different test systems. EMB had the potency to scavenge superoxide radicals, reduce iron ions, and inhibit bleaching of linoleic acid‐β‐carotene system.
    [Show full text]
  • National List of Vascular Plant Species That Occur in Wetlands 1996
    National List of Vascular Plant Species that Occur in Wetlands: 1996 National Summary Indicator by Region and Subregion Scientific Name/ North North Central South Inter- National Subregion Northeast Southeast Central Plains Plains Plains Southwest mountain Northwest California Alaska Caribbean Hawaii Indicator Range Abies amabilis (Dougl. ex Loud.) Dougl. ex Forbes FACU FACU UPL UPL,FACU Abies balsamea (L.) P. Mill. FAC FACW FAC,FACW Abies concolor (Gord. & Glend.) Lindl. ex Hildebr. NI NI NI NI NI UPL UPL Abies fraseri (Pursh) Poir. FACU FACU FACU Abies grandis (Dougl. ex D. Don) Lindl. FACU-* NI FACU-* Abies lasiocarpa (Hook.) Nutt. NI NI FACU+ FACU- FACU FAC UPL UPL,FAC Abies magnifica A. Murr. NI UPL NI FACU UPL,FACU Abildgaardia ovata (Burm. f.) Kral FACW+ FAC+ FAC+,FACW+ Abutilon theophrasti Medik. UPL FACU- FACU- UPL UPL UPL UPL UPL NI NI UPL,FACU- Acacia choriophylla Benth. FAC* FAC* Acacia farnesiana (L.) Willd. FACU NI NI* NI NI FACU Acacia greggii Gray UPL UPL FACU FACU UPL,FACU Acacia macracantha Humb. & Bonpl. ex Willd. NI FAC FAC Acacia minuta ssp. minuta (M.E. Jones) Beauchamp FACU FACU Acaena exigua Gray OBL OBL Acalypha bisetosa Bertol. ex Spreng. FACW FACW Acalypha virginica L. FACU- FACU- FAC- FACU- FACU- FACU* FACU-,FAC- Acalypha virginica var. rhomboidea (Raf.) Cooperrider FACU- FAC- FACU FACU- FACU- FACU* FACU-,FAC- Acanthocereus tetragonus (L.) Humm. FAC* NI NI FAC* Acanthomintha ilicifolia (Gray) Gray FAC* FAC* Acanthus ebracteatus Vahl OBL OBL Acer circinatum Pursh FAC- FAC NI FAC-,FAC Acer glabrum Torr. FAC FAC FAC FACU FACU* FAC FACU FACU*,FAC Acer grandidentatum Nutt.
    [Show full text]
  • TAXON:Pluchea Carolinensis SCORE:16.0 RATING:High Risk
    TAXON: Pluchea carolinensis SCORE: 16.0 RATING: High Risk Taxon: Pluchea carolinensis Family: Asteraceae Common Name(s): cattletongue Synonym(s): Conyza carolinensis Jacq. (basionym) cure-for-all Pluchea odorata auct. nonn. shrubby fleabane Pluchea symphytifolia auct. sourbush stinking fleabane sweet-scent tabat-diable wild tobacco Assessor: Chuck Chimera Status: Assessor Approved End Date: 11 May 2015 WRA Score: 16.0 Designation: H(Hawai'i) Rating: High Risk Keywords: Weedy Shrub, Aromatic, Hybridizes, Thicket-forming, Wind-dispersed Qsn # Question Answer Option Answer 101 Is the species highly domesticated? y=-3, n=0 n 102 Has the species become naturalized where grown? 103 Does the species have weedy races? Species suited to tropical or subtropical climate(s) - If 201 island is primarily wet habitat, then substitute "wet (0-low; 1-intermediate; 2-high) (See Appendix 2) High tropical" for "tropical or subtropical" 202 Quality of climate match data (0-low; 1-intermediate; 2-high) (See Appendix 2) High 203 Broad climate suitability (environmental versatility) y=1, n=0 n Native or naturalized in regions with tropical or 204 y=1, n=0 y subtropical climates Does the species have a history of repeated introductions 205 y=-2, ?=-1, n=0 y outside its natural range? 301 Naturalized beyond native range y = 1*multiplier (see Appendix 2), n= question 205 y 302 Garden/amenity/disturbance weed 303 Agricultural/forestry/horticultural weed n=0, y = 2*multiplier (see Appendix 2) y 304 Environmental weed n=0, y = 2*multiplier (see Appendix 2) y 305 Congeneric
    [Show full text]
  • Biological Studies of the Plants from Genus Pluchea
    Available online a t www.scholarsresearchlibrary.com Scholars Research Library Annals of Biological Research, 2011, 2 (3) : 25-34 (http://scholarsresearchlibrary.com/archive.html) ISSN 0976-1233 CODEN (USA): ABRNBW Biological Studies of the Plants from Genus Pluchea Surendra Kr Sharma *and Naveen Goyal Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar, India ______________________________________________________________________________ ABSTRACT Pluchea is a genus of flowering plant in the Asteraceae family. The genus Pluchea (Asteraceae) comprises 80 species distributed mainly in North and South America, Africa, Asia and Australia. These are native to tropical and warm temperate areas. The plants of Pluchea genus have been used traditionally used as astringent, antipyretic, anti-inflammatory, hepatoprotective, diaphoretic in fevers, smooth muscle relaxant, nerve tonics, laxatives and for the treatment of dysentery, lumbago, leucorrhoea, dysuria, haemorrhoids, gangrenous ulcer and disorders causing cachexia. The chemical investigations on the genus have shown the presence of eudesmane-type sesquiterpenoids, monoterpenes, lignan glycosides, triterpenoids and flavonoids. The present review summarizes the various biological studies done on the extracts and bioactive phytoconstituents from the plants of the genus Pluchea over the past few decades. Key-words: Pluchea , Biological activity, Pharmacological activities, Asteraceae. ______________________________________________________________________________
    [Show full text]
  • The Relationship Between Habitat Altitude, Enviromental Factors and Morphological Characteristics of Pluchea Indica, Ageratum Conyzoides and Elephantopus Scaber
    OnLine Journal of Biological Sciences Original Research Paper The Relationship between Habitat Altitude, Enviromental Factors and Morphological Characteristics of Pluchea Indica, Ageratum Conyzoides and Elephantopus Scaber 1,2 Yuliani, 3Soemarno, 1Bagyo Yanuwiadi and 1Amin Setyo Leksono 1Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, East Java, Indonesia 2Department of Biology, Faculty of Mathematics and Natural Sciences, Surabaya State University, Surabaya, East Java, Indonesia 3Faculty of Agriculture, University of Brawijaya, Malang, East Java, Indonesia Article history Abstract: Asteraceae family has various benefit as herbal medicine and Received: 11-05-2015 phytochemical affect (biopesticides). It can grow in different habitats but Revised: 4-06-2014 the morphological and physiological characters of Asteraceae depend on Accepted: 15-07-2015 the environmental factor. The aims of the study is to describe the variation of height of stems and width of leaves from three species of Asteraceae Correspondence Author: family ( Pluchea indica, Ageratum conyzoides and Elephantopus scaber), Yuliani on three types of habitat which differed by its altitude and learn the Department of Biology, Faculty correlation between altitude and morphological characteristics of of Mathematics and Natural Sciences, Surabaya State Asteraceae. Samples of Asteraceae were obtained from Bangkalan-Madura University, Surabaya, East (28, 3-31, 72 m asl), Trawas-Mojokerto (727-937 m asl), Coban Talun- Java, Indonesia Bumiaji Batu (1303-1322 m asl). The results were then analyzed by E-mail: [email protected] cluster analysis and Canonical Correspondence Analysis (ACA). The results show that there are correlation between altitude and environmental factors (climate and soil) to morphological characteristics especially height of stems and width of leaves of Pluchea indica, Ageratum conyzoides and Elephantopus scaber.
    [Show full text]
  • 12. Tribe INULEAE 187. BUPHTHALMUM Linnaeus, Sp. Pl. 2
    Published online on 25 October 2011. Chen, Y. S. & Anderberg, A. A. 2011. Inuleae. Pp. 820–850 in: Wu, Z. Y., Raven, P. H. & Hong, D. Y., eds., Flora of China Volume 20–21 (Asteraceae). Science Press (Beijing) & Missouri Botanical Garden Press (St. Louis). 12. Tribe INULEAE 旋覆花族 xuan fu hua zu Chen Yousheng (陈又生); Arne A. Anderberg Shrubs, subshrubs, or herbs. Stems with or without resin ducts, without fibers in phloem. Leaves alternate or rarely subopposite, often glandular, petiolate or sessile, margins entire or dentate to serrate, sometimes pinnatifid to pinnatisect. Capitula usually in co- rymbiform, paniculiform, or racemiform arrays, often solitary or few together, heterogamous or less often homogamous. Phyllaries persistent or falling, in (2 or)3–7+ series, distinct, unequal to subequal, herbaceous to membranous, margins and/or apices usually scarious; stereome undivided. Receptacles flat to somewhat convex, epaleate or paleate. Capitula radiate, disciform, or discoid. Mar- ginal florets when present radiate, miniradiate, or filiform, in 1 or 2, or sometimes several series, female and fertile; corollas usually yellow, sometimes reddish, rarely ochroleucous or purple. Disk florets bisexual or functionally male, fertile; corollas usually yellow, sometimes reddish, rarely ochroleucous or purplish, actinomorphic, not 2-lipped, lobes (4 or)5, usually ± deltate; anther bases tailed, apical appendages ovate to lanceolate-ovate or linear, rarely truncate; styles abaxially with acute to obtuse hairs, distally or reaching below bifurcation,
    [Show full text]
  • On the Flora of Australia
    L'IBRARY'OF THE GRAY HERBARIUM HARVARD UNIVERSITY. BOUGHT. THE FLORA OF AUSTRALIA, ITS ORIGIN, AFFINITIES, AND DISTRIBUTION; BEING AN TO THE FLORA OF TASMANIA. BY JOSEPH DALTON HOOKER, M.D., F.R.S., L.S., & G.S.; LATE BOTANIST TO THE ANTARCTIC EXPEDITION. LONDON : LOVELL REEVE, HENRIETTA STREET, COVENT GARDEN. r^/f'ORElGN&ENGLISH' <^ . 1859. i^\BOOKSELLERS^.- PR 2G 1.912 Gray Herbarium Harvard University ON THE FLORA OF AUSTRALIA ITS ORIGIN, AFFINITIES, AND DISTRIBUTION. I I / ON THE FLORA OF AUSTRALIA, ITS ORIGIN, AFFINITIES, AND DISTRIBUTION; BEIKG AN TO THE FLORA OF TASMANIA. BY JOSEPH DALTON HOOKER, M.D., F.R.S., L.S., & G.S.; LATE BOTANIST TO THE ANTARCTIC EXPEDITION. Reprinted from the JJotany of the Antarctic Expedition, Part III., Flora of Tasmania, Vol. I. LONDON : LOVELL REEVE, HENRIETTA STREET, COVENT GARDEN. 1859. PRINTED BY JOHN EDWARD TAYLOR, LITTLE QUEEN STREET, LINCOLN'S INN FIELDS. CONTENTS OF THE INTRODUCTORY ESSAY. § i. Preliminary Remarks. PAGE Sources of Information, published and unpublished, materials, collections, etc i Object of arranging them to discuss the Origin, Peculiarities, and Distribution of the Vegetation of Australia, and to regard them in relation to the views of Darwin and others, on the Creation of Species .... iii^ § 2. On the General Phenomena of Variation in the Vegetable Kingdom. All plants more or less variable ; rate, extent, and nature of variability ; differences of amount and degree in different natural groups of plants v Parallelism of features of variability in different groups of individuals (varieties, species, genera, etc.), and in wild and cultivated plants vii Variation a centrifugal force ; the tendency in the progeny of varieties being to depart further from their original types, not to revert to them viii Effects of cross-impregnation and hybridization ultimately favourable to permanence of specific character x Darwin's Theory of Natural Selection ; — its effects on variable organisms under varying conditions is to give a temporary stability to races, species, genera, etc xi § 3.
    [Show full text]
  • Lepidoptera: Arctiidae), and Potential for Biological Control of Senecio Madagascariensis (Asteraceae) M
    J. Appl. Entomol. Host range of Secusio extensa (Lepidoptera: Arctiidae), and potential for biological control of Senecio madagascariensis (Asteraceae) M. M. Ramadan1, K. T. Murai1 & T. Johnson2 1 State of Hawaii Department of Agriculture, Plant Pest Control Branch, Honolulu, Hawaii, USA 2 Institute of Pacific Islands Forestry, Pacific Southwest Research Station, USDA Forest Service, Volcano, Hawaii, USA Keywords Abstract Host range, Secusio extensa, Senecio madagascariensis Secusio extensa (Lepidoptera: Arctiidae) was evaluated as a potential bio- logical control agent for Madagascar fireweed, Senecio madagascariensis Correspondence (Asteraceae), which has invaded over 400 000 acres of rangeland in the Mohsen M. Ramadan (corresponding author), Hawaiian Islands and is toxic to cattle and horses. The moth was intro- State of Hawaii Department of Agriculture, duced from southeastern Madagascar into containment facilities in Plant Pest Control Branch, 1428 South King Hawaii, and host specificity tests were conducted on 71 endemic and Street, Honolulu, Hawaii 96814, USA. E-mail: [email protected] naturalized species (52 genera) in 12 tribes of Asteraceae and 17 species of non-Asteraceae including six native shrubs and trees considered key Received: September 15, 2009; accepted: components of Hawaiian ecosystems. No-choice feeding tests indicated April 6, 2010. that plant species of the tribe Senecioneae were suitable hosts with first instars completing development to adult stage on S. madagascariensis doi: 10.1111/j.1439-0418.2010.01536.x (78.3%), Delairea odorata (66.1%), Senecio vulgaris (57.1%), Crassoceph- alum crepidioides (41.2%), and at significantly lower rates on Emilia fos- bergii (1.8%) and Erechtites hieracifolia (1.3%). A low rate of complete larval development also was observed on sunflower, Helianthus annuus (11.6%), in the tribe Heliantheae.
    [Show full text]
  • Evolutionary Relationships in the Asteraceae Tribe Inuleae (Incl
    ARTICLE IN PRESS Organisms, Diversity & Evolution 5 (2005) 135–146 www.elsevier.de/ode Evolutionary relationships in the Asteraceae tribe Inuleae (incl. Plucheeae) evidenced by DNA sequences of ndhF; with notes on the systematic positions of some aberrant genera Arne A. Anderberga,Ã, Pia Eldena¨ sb, Randall J. Bayerc, Markus Englundd aDepartment of Phanerogamic Botany, Swedish Museum of Natural History, P.O. Box 50007, SE-104 05 Stockholm, Sweden bLaboratory for Molecular Systematics, Swedish Museum of Natural History, P.O. Box 50007, SE-104 05 Stockholm, Sweden cAustralian National Herbarium, Centre for Plant Biodiversity Research, GPO Box 1600 Canberra ACT 2601, Australia dDepartment of Systematic Botany, University of Stockholm, SE-106 91 Stockholm, Sweden Received27 August 2004; accepted24 October 2004 Abstract The phylogenetic relationships between the tribes Inuleae sensu stricto andPlucheeae are investigatedby analysis of sequence data from the cpDNA gene ndhF. The delimitation between the two tribes is elucidated, and the systematic positions of a number of genera associatedwith these groups, i.e. genera with either aberrant morphological characters or a debated systematic position, are clarified. Together, the Inuleae and Plucheeae form a monophyletic group in which the majority of genera of Inuleae s.str. form one clade, and all the taxa from the Plucheeae together with the genera Antiphiona, Calostephane, Geigeria, Ondetia, Pechuel-loeschea, Pegolettia,andIphionopsis from Inuleae s.str. form another. Members of the Plucheeae are nestedwith genera of the Inuleae s.str., andsupport for the Plucheeae clade is weak. Consequently, the latter cannot be maintained and the two groups are treated as one tribe, Inuleae, with the two subtribes Inulinae andPlucheinae.
    [Show full text]
  • Antitumor Activity and Antioxidant Role of Tissue-Cultured Pluchea Indica Root Against Ehrlich Ascites Carcinoma in Swiss Albino Mice
    ® International Journal of Biomedical and Pharmaceutical Sciences ©2008 Global Science Books Antitumor Activity and Antioxidant Role of Tissue-Cultured Pluchea indica Root against Ehrlich Ascites Carcinoma in Swiss Albino Mice Kartick Chandra Pramanik • Sanchita Ghosh • Dilip Kumar Midya • Tapan Kumar Chatterjee* Department of Pharmaceutical Technology, Division of Pharmacology, Jadavpur University, Kolkata 700 032, India Corresponding author : * [email protected] ABSTRACT One of the major achievements of medical science has been the control and management of infectious diseases. The present study was designed to evaluate the antitumor effect and antioxidant role of methanolic root extract of tissue cultured Pluchea indica. Antitumor activity and antioxidant status of this extract (100 and 300 mg/kg) were evaluated against Ehrlich Ascites Carcinoma (EAC) cells in mice. After 24 h of tumor inoculation, the root extract was administered orally daily for 14 days. After administered of the last dose followed by 18 h fasting, mice were then sacrificed for observation of anti-tumor activity. The effect of methanolic extract of P. i nd i ca (MEPI) on the growth of transplantable murine tumor and life span of EAC bearing hosts were estimated. Simultaneously the hematological values and liver biochemical parameters (lipid peroxidation, antioxidant enzymes) were estimated in treated animals. MEPI decreased mice weight, tumor volume, viable cell count and amounted to 19.25 g, 1.87 ml, and 24.00 × 106 cells/ml, respectively when compared to EAC control. MEPI also showed an increase in the non-viable cell count (55.25 × 106 cells/ml) and mean survival time of 26.50 days thereby increasing the life span by 62.27% more than EAC tumor-bearing mice.
    [Show full text]
  • Addition of Five New Generic Asteraceous Members to the Flora of Odisha, India
    J. Indian bot. Soc. e-ISSN:2455-7218, ISSN:0019 - 4468 Vol. 100 (1&2) 2020:53-61 ADDITION OF FIVE NEW GENERIC ASTERACEOUS MEMBERS TO THE FLORA OF ODISHA, INDIA G S J P JENA1, RAMAKANTA MISHRA2, SMARANIKA NAYAK2 AND KUNJA BIHARI SATAPATHY3 1Department of Botany, S.G. College, Kanikapada, Jajpur - 755011, Odisha ,2P.G. Department of Botany, Bio systematic Laboratory, Utkal University, VaniVihar, Bhubaneswar- 751004, Odisha 3School of Applied Sciences, Centurion University of Technology and Management, Bhubaneswar- 752050, Odisha Email: [email protected] Date of online publication: 30th September DOI:10.5958/2455-7218.2020.00025.X The proper taxonomic identification presumes first and foremost priority in the scientific documentation of biodiversity. During the present floral inventory five unreported Asteraceous members were recorded from different parts of Odisha. They are Baccharis salicifolia (Ruiz & Pavón) Pers., Calyptocarpus vialis Less., Cirsium arvense (L.) Scop. var. arvense, Pluchea indica (L.) Less., and Pseudelephantopus spicatus (Juss. ex Aubl) C.F. Baker. A detailed botanical description along with notes on nomenclature, ecology, geographic distribution and coloured photographs of each species have been provided to facilitate easy identification in the field. On close verification of herbarium specimens and proper scrutiny of literature published till date on these taxa, it can be proclaimed that these are new addition to the Flora of Odisha. Key words: New record, Asteraceae, Flora of Odisha Now-a-days systematic documentation of documentation and investigation of new taxa in phytodiversity has presumed instantaneous any area is of prime importance. The present research priority (Singh 2012). To this end, investigation was intended to document and preliminary research priority is to report the identify the Asteraceous unreported new taxa biological diversity and distribution of various in the areas under study namely Jajpur, life forms at local, regional, and global scales Keonjhar and Angul districts of Odisha.
    [Show full text]