ACAR1NES, 10:9-12,2016

A New Species of the Genus (: ) from Egypt

M. F. Hassan*, H. A. Taha** and M.A. K. Nasr** */.oology and Agric. Nematology Dept.. Faculty of Agric .. Cairo Univ .. Egypt. "Plant Protec. Res. lnst.. ;\gric. Res. Center. Dokki. CiiL

ABSTRACT

Asca aegyptiaca sp. nov., is desc1ibed ti·om Egypt, the genus is recorded tor the tirst time and collected ti·om leaves and debris of soybean at Mcnia governorate (Midle Egypt).

Key words: Mesostigmata; Ascidae; Asca; new species; soybean.

INTRODUCTION measured from base of coxae to tips of tarsi (without pretarses ). The term "pore" and "fissure" used in of the genus Asca Heyden, are, predatory descriptions are applied to all pore - like structures mites, feed on various and entomogenous and includes both sense organs and glands. species of nematodes. (Hurlbutt 1963, 1971; Walter 1988; Epsky eta!., 1988 and Walter& Proctor, 1999). RESULTS AND DISCUSSION Species of this genus are inhabiting leaf litter, soil moss-covered tree trunks in bark beetle gallareis Genus: Asca von Heyden (Stone & Simpson 1991), termite's and bird's nests Asca von Heyden, 1826: 610. Type species (Ryke 1961and Lindquist et al., 2009). Karg (1979) Gamasus aphidisides Fabricius, 1805, by original reviewed the genus Asca and mentioned that 75 designation (=Acarus aphidioides L., 1728) species were presented in the world, at that time and Ceratozercon Berlese, 1910: Type species: Zercon gave a key for various species. Many species have bicornis Berlese, 1887 (not Gamasus (Sejus) bicornis been described and illustrated by Tseng ( 1981 ); Canestrini & Fanzango, 1876) by subsequent Walter et al., (1993); Lee et al., (1997) and Beard et designation (Berlese 1913) (=Acarus aphidioides L., al., (2011 ). 1758). Synonomy by Vitzthum ( 1929) cited by Halliday et al., 1998: 17. In Eb'YPt, genus Asca has been recorded for the first time by a new species Asca aegyptiaca sp. nov. Diagnosis: Dorsal shield divided, with pair of collected from leaves and debris of soybean at posterior projection (horns) on opisthosomal shied

Mallawy, Menia governorate, (Midle-Egypt).The usually bearing setae Z4 and S5 . Female with description and illustrations of this new species are expanded ventrianal shield incorporating 5-6 pairs of given here. ventral setae (usually Jv2_5, Zv2_3) in addition to circumanal setae. Genu l lacks ventral setae av2 (and MATERIALS AND METHODS so having 12 rather than 13 setae). Third pair of sternal pores (stp3) on the sternal shield; fourth pair of

Leaf samples were bagged and placed in an ice sternal setae (st4 ) free on soft cuticle. Movable digit box until examined. Mites were collected with a fine of chelicerae usually bidentate. camel-hair brush, and cleared in Nesbitt's solution before mounting in Hoyer's medium on microscope A sea aegyptiaca s p. nov. slides. Measurements in micrometers (!JI11) were Diagnosis: This species is sirniller to A. garmani made from specimens flattened on microscope slides Hurlbutt, 1963. but differes mainly in: setae J 4 and Zs using a stage-calibrated ocular micrometer and are slightly barbed, whearse seta J 4 coarsly barbed presented in parentheses as ranges (minimum to and seta Z5 smooth in A. garmani; there is procurved maximum). Lengths of shields were measured along ridge between setae j5, whearse this character is their midlines, and width between coxae II -III or at absent in A. aegyptiaca; fixed digit of chelicera their widest point (ventral -anal, dorsal). Setae were having 7 teeth in A. aegyptiaca, whearse A. garrmni measured from the bases of their insertions to their has 5-6 teeth; setae ad2 and pd2 on tarsus IV (Fig. 9) tips and distance between setae as the distance whip-like in A. aegyptiaca, whears in A. garmani between their insertion.<>. The system of setal notation simple. for the idiosomal and the generic concept of Asca are based on that of Lindquist and Evans ( 1965). The Description: Female (Figs. 1-9) light brown, anterior notation used for leg setation follows that of Evans dorsal shield (140-155) !JI11 long, (133-147) ~m ( 1963). Movable chelicera) digits were measured width; bearing 17 pairs of coarsely barbed setae from their basal articulation to their tips. Legs (j, -j6, z2- 26 and s,- s6) setae r2-rs inserted in soft 10

I19. •. un SOJJm

u

Zv1

Fig.( I): Asca aegyptiaca sp. nov. dorsal view. Fig.(2): Asca aegyptiaca sp. nov . ventral view.

I

7 Zs

3

Figs.(3-9): A sea aegyptiaca sp. nov. (3) subcapitulum ( 4) chelicera (5) tectum (6) seta S5 (7) seta Z5 (8) seta j 3 (9) tarsus. 11 cuticle laterally; shield generally smooth. except vvith tooth. Palp setation of trochanter- tibia (normal for scattered reticulations, and some scatter rows of the genus) 2-5-6-12; palp apotcle two-tined, minute protuberances on area between setae j4 and z< subcapitulum (Fig: 3) with 7 rows of 8-10 of it appears to have six pairs of pore-like structures. deutoste rnal denticles : corniculi horn - like. curved distributed as in Fig. I. Measurements of setae: j 1 =j2 internally with sharp tip, setae h1 subequal to h:; and ; := r2 :==(14-18!lm),j3:=j6 :=s 1=:Z4:=s 4 :=s 5 := r5 = (16- C. s and longer than h2 all simple. 19 !lin) j4- j = z =rs = s = (17-20 ~un). Posterior 5 5 6 Tectum (Fig. 5): Apex with 3 equal tines, each dorsal shield (129 -142 ~n) long (143 - 155Mm) with denticulated tips. Setation of legs 1-JV: Coxae width, smooth, except some oblique rows of minute 2-2-2- 1, trochanters 6-5 5-5, femora 12-10-6-6, ge nua protuberances between J series and Z; bearing 15 12- 11-8-9, tibiae 13-10-8-10, tarsi II -IV 17-17-17; pairs differ in length (J -J , Z -Z and S -S ) and 1 5 1 5 1 5 Genua Ill seta al2 present, tarsus IV (Fig. 9) setae barbed setae; postero-lateral horns bearing pinnate ab2 and pd2. flagellated (50-58 ~un) long (excluding conspicuous S (32-35 ~) long, and minute, 5 pretarsus). vestigial Z4 ; setae J5 minute (2-4 !lin) 6 long. Setae R1-R., and UR 1 coarsely barbed, and inse1ted in soft Male: unknown. cuticle laterally; length of setae J 1= (18-20 ~m1). Jz=J3 (24-26~n), ]4 (33-37 ~), zl (18-23 ~n) z2 Remarks: According to the concept of karg ( 1979). (26-30 ~n), z3(35-39 !lin) Zs ( 34-39 ~un), sI ( 14-16 A. aegyptiaca sp. nov., belongs to Asca aphidioides spec ies group, which charactized by: having a single ~n) S2 (24-27 ~n) , S3 (29-31 ~n), R2 (18-20 ~un) , large seta on each posterior horn (another minute seta R2 = R3 = R4 = R5 = R6 (18-22 ~) R 1 (10-13 ~un) , is also present), minute seta .1 , an essentially setae 14 , Z3 , Z5 , S2 S3 and S4 are slightly barbed; while 5 the remain setae are coarse ly barbed. Distances triramous apex ta the tectum, and small tooth like tubercles on the posterior dorsal shield, Walter et al between sockets of setae as: J ~-J 1= .I 1-]2 (25-28 11m), 1r12 (26-30 ~) J4-J 4= J4-Z3 (35-28 ~n) ; a row of (1993). Asca aegyptiaca is close to A. garmani, A. dentition at the posterior end of the shield is prese nL afroaphidioides Hurlbutt, 1971 and A. aethiopica Ryke, 1961. About the differences between A. Peritreme extends to level seta j2; peritrematal shield garmani and the new species, is mentioned with fused to dorsal shield from r2 to level of j 2 anterolaterally. diagnoses; while A. afroaphidioides differs mainly by setae S4, Z5 and R series being simple , whereas in the

Ventral idiosoma (Fig. 2): Jugular is indistinct, bears new species, setae S4 and Z5 are slightly barbed and R seta st~o sternal shield, smooth medially, weakly series are coarsely barbed ; A. aethiopica with sclerotized, undefined antieriotly and with slightly ornamentated anterior dorsal shield, whereas in A. concave posterior margin, bearing st2 and st3 , having aegyptiaca this shield seems to be smooth except 3 pairs of pore-like structure, with oblique some scattered reticulations and some rows of minute longitudinal lines laterally; distances of st 1-st2 (31-35 protuberances on area between setae j4 and ~ ' also

~n), strst2 (33-37~) and st2 -st3 ( 40-45~n) setae s~ seta J4 sub equal to J, and both coarsely barbed on soft cubical free posterolaterally of sternal shield. whereas inA. aegyptiaca ]4 slightly barbed and longer Metasternal shield absent. Genital shield, smooth, than JJ (coarsely barbed). truncate posterior margin, bearing a pair of genital Material Examined: seta(st5). Two pairs ofventral setae(Jv~o Zv 1) and a pair of pores in plicate soft cuticle anterior to Holotype: Collected from leaves of soybean (Glycine ventrianal shield, two pairs of circular metapodal max (L.) on 13 October, 2014. platelets, outer one larger. Ventrianal shield, with Paratypes: Four females, with the same data of slightly concaved anterior margin, reticulated by 4 holotype. transverse rows and connected with little rows REFERENCES laterally, bearing 6 pairs of ventral setae (Jv 2 _ 5 , Zv 2, Zv3); setae .lv4 and Jv5 curved and finely barbed; postanal seta thick, barbed and longer than para-anals. Beard, .1.; Ochoa, R. and Vega, F. E. 2011. A new Distances between sockets of some setae: strs~ Asca (: Mesostigmata: Ascidae) from Costa Rica. Syst. Appl. Acarol., 16(1): 7-20. (39-45~) st5 -st5 (44-45~), Jv1-Jv1 (24-28~tm) , Epsky, N. D.; Walter, D. E. and Capinera, J. L. Zv2-Zv2 (75-80~), Jvr Jv3 (29-33 ~) , Zv 3 -Zv3 1988. Potential role of nematophagous (136-144~) and Jv5-Jv5 (90-98 ~). Spermathecal apparatus indistinct. microarthropods as biotic mo1tality factors of entomogenous nematodes (Rhabditida: Gnathosoma (Fig. 3): Movable digit of chelicerae Steinemematidae, Heterorhabditidae ). J. Econ. (22-25 ~) with two large teeth while fixed digit Entomol., 81 :821-825. (20-22 ~) with a row of 6-7 teeth, short, sharp Eva ns, G. 0. 1963. Observations on the chetotaxy of setiform pilus dentilis, and subapical off-set bifid the legs in the free-living Gmasina (Acari: 12

Mesostigmata). Bull. Brit. Mus. (Nat. ~li s t.) Gamosellu.1· a nd Asca. with supplementa l Zooi. , IO: 277-303 description of known species of the genus Asca Ha lliday. R. B.: Walter, D. E. and Lindquist, E. (Acari: Mesostigmata, Rhodacaridae ). Acta E. 1998. Revision of the Australian Ascidae Zootaxomica Sinica, 30 (3): 538-544. (Acarina: Mesostigmata). Inve11ebrate Ryke, P. A. J. 1961. A review of the genus A sea von , 12: 154. Heyden with descriptions of new species Hurlbutt, H. W. 1963. The genus Asca Heyden (Acarina: Mesostigmata: (Acarina: Mesostigmata) in North America, Rhodacaridae). Zoologischer Anzeiger, 167: 127- Hawaii and Europe. Acarologia, 5: 480-518. 135. Hurlbutt, H. W. 1971. Ascinae and Podocinae Stone, C. and Simpson, J. A. 1991. Effect of six (Acarina: Mesostigmata) from Tanzania. chemicals on the insects, mites, nematodes and Acarologia, 13 (2): 280-300. fungi associated with Ips grandicollis (Eichhoff) Karg, W. 1979. Die Gattung Hypoaspis Canestrini, (Coleoptera: Scolytidae) in Northeastern New 1884 (Acarina, Paras itiformes ). Zoologisc he South Wales. Journal of the Australian Jahrbi.icher Abteilung fl.ir Systematik, Okolog ie Entomological Society, 30: 21-28. und Geographie der Tiere, 106: 65-104. Tseng, Y. H. 1981. The mite family Ascidae from(ll) Lee, W. K.; Lee, S. Y. and Ryu, M. 0.1997. -Genus Asca Heyden(Acarina: Mesostigmabnta). Taxonomic Studies on the genus Asca (Ascidae: J. Entomol., 2: 7- 25. Mesostigmata) in Korea ll. Description of two Waiter, D. E. ; Halliday, R. B. and Lindquist, E. E. new species. The Korean Journa l of Systematic 1993. A review of the genus Asca (Acarina: Zoology, 13(1): 33- 44. Ascidae) in Australia, with the description of three Lindquist, E. E. and Evans, G. 0. 1965. Taxonomic new leaf inhabiting spec1es. Invertebrate concepts in the Ascidae, with a modified setal Taxonomy,7: 1327-1347. nomenclature for the idiosoma of the Gamasina Walter, D. E. 1988. N ematophat,'Y by soil a11hropods (Acarina: Mesostigmata). Memoirs of the from the shortgrass steppe, Chihuahuan desert and Entomological Society of Canada, 47:1-59. Rocky Mountains of the Central United Lindquist, E. E.; Krantz, G. W. and Walter, D. E. States. Agriculture, Ecosystems and Environment, 2009. Order Mesostigmata. In: Krantz, G. W. and 24: 307-316. Walter, D. E. (Eds.), A Manual of Acarology. Walter, D. E.andProctor, H. C.l999.Mites: Texas Tech University Press, USA, 124-232. Ecology, Evolution and Behaviour. University of Ma, L. M. 2005. New species of the genera New South Wa les Press, Sydney, Australia.