Supporting Information for Proteomics DOI 10.1002/Pmic.200601006

Supporting Information for Proteomics DOI 10.1002/pmic.200601006

Chenggang Wu, Mike Haiting Ma, Kevin R. Brown, Matt Geisler, Lei Li, Eve Tzeng, Christina Y. H. Jia, Igor Jurisica and Shawn S.-C. Li

Systematic identification of SH3 domain-mediated human protein–protein interactions by peptide array target screening

ª 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.proteomics-journal.com

SUPPLEMENTARY MATERIALS

Systematic Identification of SH3 Domain-mediated Human Protein-protein

Interactions by Peptide Array Target Screening

Chenggang Wu1,*, Mike Haiting Ma1,*, Kevin R Brown2, Matt Giesler1, Lei Li1, Eve

Tzeng1, Christina Y.H. Jia1, Igor Jurisica2 and Shawn S.-C. Li1

1Department of Biochemistry, Schulich School of Medicine and Dentistry, University of

Western Ontario, London, Ontario N6A 5C1; 2Ontario Cancer Institute, Northeast

Structural Genomics Consortium, 610 University Avenue, Toronto, Ontario M5G 2M9,

Canada.

SUPPLEMENTARY TABLES

Table S1: PLCγ1 interacting proteins identified by PATS.

Table S2: List of SH3-interacting proteins identified by PATS.

SUPPLEMENTARY FIGURE LEGENDS

Figure S1. Fluorescein-labeled SH3 domain displayed similar affinities for their

peptide targets as the wild-type proteins. A peptide, YEDTPPPLPPKPKFR, derived from HPK1 and shown to bind to both the PLCγ1 SH3 and the Grb2 SH3N domain was

chemically synthesized and labeled at the N-terminus with biotin. The biotinylated

peptide was mixed with either the wt or fluorescein-labeled SH3 domain in solution, and the resulting peptide-SH3 complex was pulled down by streptavidin-agarose beads and

resolved on SDS-PAGE. The figure shows that fluorescein-labeling (via a C-terminal

Gly-Gly-Cys triad) does not affect binding of an SH3 domain to its target peptide. f-SH3, fluorescein-labeled SH3; Bt-peptide, biotinylated peptide.

Figure S2. Overlay between PATS and OPHID SH3 interaction networks. A network mediated by the 8 SH3 proteins used in the present study was extracted from the OPHID database and overlaid onto the PATS SH3 network identified for the same proteins. The 8

SH3 “bait” proteins are depicted as ovals whereas their binding proteins are represented

by either small solid circles or diamonds for those containing at least one SH3 domain of

their own. Connecting edges denote binary interactions with grey for PATS interactions,

red for OPHID interactions, and blue for overlapping interactions.

Table S1. PLCγ1 interacting proteins identified by PATS

SPOT Normalized Peptide SwissProt Peptide ID Signal Sequence Protein ID Position Protein Description

1366 9.421927749 QMEGPHLPNKKKHK ICAL_HUMAN (14-27) Calpastatin (Calpain inhibitor) (Sperm BS-17 component).

1524 7.659564051 VADTPEVPIKKHFK DUS15_HUMAN (52-65) Dual specificity protein phosphatase 15 (EC 3.1.3.48) (EC 3.1.3.16). (214- 1436 7.003174156 SERRPQAPAKKLRK DLX4_HUMAN 227) Homeobox protein DLX-4 (DLX-7) (DLX-8) (Fragment). (433- 732 6.73707784 VMIRPLPVKPKTK TRAIP_HUMAN 445) TRAF-interacting protein.

810 6.641601661 ANVLPAFPPKKLFS SNX17_HUMAN (53-66) Sorting nexin 17. (462- 506 6.421857193 PTLKYFPAGPGRK PDIA2_HUMAN 474) Protein disulfide-isomerase A2 precursor (EC 5.3.4.1) (PDIp). (557- Solute carrier family 23, member 1 (Sodium-dependent vitamin Ctransporter 1) (hSVCT1) 676 6.319769415 TFLKYIPICPVFK S23A1_HUMAN 569) (Na(+)/L-ascorbic acid transporter 1) (Yolksac permease-like molecule 3). (781- Development and differentiation-enhancing factor 2 (Pyk2 C-terminusassociated protein) (PAP) 1520 6.223511341 TTSAPPLPPRNVGK DDEF2_HUMAN 794) (Paxillin-associated protein with ARFGAPactivity 3) (PAG3). (504- 86 5.960827351 DKDKWLPFFPKTK ARHGB_HUMAN 516) Rho guanine nucleotide exchange factor 11 (PDZ-RhoGEF). Exostosin-like 3 (EC 2.4.1.223) (Glucuronyl-galactosyl-proteoglycan 4-alpha-N- (329- acetylglucosaminyltransferase) (Putative tumor suppressorprotein EXTL3) (Multiple exostosis- 1183 5.810539507 MEIPPQVPVKRKYL EXTL3_HUMAN 342) like protein 3) (Hereditarymultiple exostoses gene isolog) (EXT-related protein 1). (148- 1365 5.723381058 QLTGPVMPVRNVYK RL13_HUMAN 161) 60S ribosomal protein L13 (Breast basic conserved protein 1) (OK/SW-cl.46). 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein (PIP2- (614- dependent ARF1 GAP) (ADP-ribosylation factor-directed GTPase-activating protein 1) (ARF 1515 5.706390185 TTEAPPLPPRNAGK DDEF1_HUMAN 627) GTPase-activating protein1) (Development and differentiation-enhancing factor 1) (Fragment). (199- 1305 5.394439639 PPSPPSAPRKKGGG RRAS_HUMAN 212) Ras-related protein R-Ras (p23). (391- Mitogen-activated protein kinase kinase kinase kinase 1 (EC 2.7.1.37)(MAPK/ERK kinase kinase 892 5.383207169 EDTPPPLPPKPKFR M4K1_HUMAN 404) kinase 1) (MEK kinase kinase 1) (MEKKK 1)(Hematopoietic progenitor kinase). (617- 1472 5.372543475 SSGSPALPRKQRDK ABL2_HUMAN 630) Tyrosine-protein kinase ABL2 (EC 2.7.1.112) (Tyrosine kinase ARG).

426 5.282967813 NRVKLLPAGPAPK EFS_HUMAN (61-73) Embryonal Fyn-associated substrate (HEFS). (539- Growth hormone receptor precursor (GH receptor) (Somatotropinreceptor) [Contains: Growth 71 5.219909526 DAKKCIPVAPHIK GHR_HUMAN 551) hormone binding protein (GH bindingprotein) (GHBP) (Serum binding protein)]. (4521- Ryanodine receptor 1 (Skeletal muscle-type ryanodine receptor) (RyR1)(RYR-1) (Skeletal 474 5.022629911 PPKKQAPPSPPPK RYR1_HUMAN 4533) muscle calcium release channel). (470- 430 4.87127565 NVGRNIPIFPCHK RGS7_HUMAN 482) Regulator of G-protein signaling 7 (RGS7). (944- 1200 4.85641749 MVDKPPVPPKPKMK SHAN2_HUMAN 957) SH3 and multiple ankyrin repeat domains protein 2 (Shank2). (813- 1357 4.813051573 QGQSPPAPTRGISK CHTOG_HUMAN 826) CH-TOG protein (Colonic and hepatic tumor over-expressed protein) (Ch-TOG protein). (1143- 1282 4.786889811 PLIPPPLPPRKKFD SOS2_HUMAN 1156) Son of sevenless protein homolog 2 (SOS-2). (494- 516 4.750633023 QFTKCCPPKPKDK JAK2_HUMAN 506) Tyrosine-protein kinase JAK2 (EC 2.7.1.112) (Janus kinase 2) (JAK-2). (1021- 1278 4.727172812 PKPLPRFPKKYSYP SOS1_HUMAN 1034) Son of sevenless protein homolog 1 (SOS-1). Filamin B (FLN-B) (Beta-filamin) (Actin-binding-like protein) (Thyroidautoantigen) (240- (Truncated actin-binding protein) (Truncated ABP) (ABP-280 homolog) (ABP-278) (Filamin 3) 795 4.70783585 AKLKPGAPLKPKLN FLNB_HUMAN 253) (Filamin homolog 1) (Fh1).

572 4.687219188 RLRRLVPTIPPNK ID4_HUMAN (74-86) DNA-binding protein inhibitor ID-4. (267- Filamin A (Alpha-filamin) (Filamin 1) (Endothelial actin-bindingprotein) (Actin-binding 796 4.66333244 AKLKPGAPLRPKLN FLNA_HUMAN 280) protein 280) (ABP-280) (Nonmuscle filamin). (330- 733 4.647265683 VMQRLFPRIPHMK IL3RA_HUMAN 342) Interleukin-3 receptor alpha chain precursor (IL-3R-alpha) (CD123antigen). (606- 299 4.619539903 KKKKTAPTPPKRS ABL1_HUMAN 618) Proto-oncogene tyrosine-protein kinase ABL1 (EC 2.7.1.112) (p150) (c-ABL). Histone-lysine N-methyltransferase, H3 lysine-9 specific 2(EC 2.1.1.43) (Histone H3-K9 (233- methyltransferase 2) (H3-K9-HMTase 2)(Suppressor of variegation 3-9 homolog 2) (Su(var)3-9 843 4.282423009 CQCGPDCPNRIVQK SUV92_HUMAN 246) homolog 2). (1135- 734 4.280290235 VNDRIVPQPPLQK SC24B_HUMAN 1147) Protein transport protein Sec24B (SEC24-related protein B). (274- Zona pellucida sperm-binding protein 2 precursor (Zona pellucidaglycoprotein ZP2) (Zona 1505 4.220786486 TLTIPEFPGKLKSV ZP2_HUMAN 287) pellucida protein A). Low affinity immunoglobulin gamma Fc region receptor II-b precursor(Fc-gamma RII-b) (FcRII- 1526 4.214885865 VAGTPAAPPKAVLK FCG2B_HUMAN (40-53) b) (IgG Fc receptor II-b) (Fc-gamma-RIIb)(CD32 antigen) (CDw32). Low affinity immunoglobulin gamma Fc region receptor II-c precursor(Fc-gamma RII-c) (FcRII- 1526 4.214885865 VAGTPAAPPKAVLK FCG2C_HUMAN (40-53) c) (IgG Fc receptor II-c) (Fc-gamma-RIIc)(CD32 antigen) (CDw32). (153- 1240 4.097726672 PALPPSAPPKPRSK PHAR2_HUMAN 166) Phosphatase and actin regulator 2. (342- 1181 4.085143492 MCPDPGIPERGKRL CSMD2_HUMAN 355) CUB and sushi multiple domains protein 2.

18 4.07547503 AKPRDIPMNPMCI ANT3_HUMAN (42-54) Antithrombin-III precursor (ATIII) (PRO0309). (1404- 1149 4.022867084 LQLIPRCPKKSFSH FANCA_HUMAN 1417) Fanconi anemia group A protein (FACA protein). (248- Trans-Golgi network integral membrane protein 2 precursor (Trans-Golginetwork protein TGN51) 648 4.005876216 SPNKVVPEQPSRK TGON2_HUMAN 260) (TGN46) (TGN48) (TGN38 homolog). (472- SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated 1127 4.004880924 LLEKPPVPPKPKLK SHAN3_HUMAN 485) protein 2) (ProSAP2) (Fragment). (233- Chromatin assembly factor 1 subunit A (CAF-1 subunit A) (Chromatinassembly factor I p150 486 3.974595821 PQIKSLPATPQGK CAF1A_HUMAN 245) subunit) (CAF-I 150 kDa subunit) (CAF-Ip150). (688- 477 3.931940767 PPNKELPPSPEKK MAP4_HUMAN 700) Microtubule-associated protein 4 (MAP 4).

1523 3.855943806 TWKTPSFPKKKTAA NLEX_HUMAN (70-83) Probable nucleolar exonuclease (EC 3.1.-.-). (183- 637 3.837104429 SLMRLCPIIPKLL TDRD1_HUMAN 195) Tudor domain containing protein 1. (685- Fragile X mental retardation 2 protein (Protein FMR-2) (FMR2P) (Ox19protein) (Fragile X E 1402 3.806179578 RPNIPLAPEKKKYR FMR2_HUMAN 698) mental retardation syndrome protein). (122- Autoimmune regulator (Autoimmune polyendocrinopathy candidiasisectodermal dystrophy protein) 1170 3.793383061 LVPPPRLPTKRKAS AIRE_HUMAN 135) (APECED protein). (139- 672 3.702598863 TARKKAPPPPKRA SHAN2_HUMAN 151) SH3 and multiple ankyrin repeat domains protein 2 (Shank2). (441- 1414 3.61437415 RSLKPVLPHKVAHF DNL3_HUMAN 454) DNA ligase III (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP]). (115- 70 3.590274049 CVMKHLPKVPEKK NELFB_HUMAN 127) Negative elongation factor B (NELF-B) (Cofactor of BRCA1). (370- 1377 3.547050283 QVPAPPLPAKKNLP RIN3_HUMAN 383) Ras and Rab interactor 3 (Ras interaction/interference protein 3).

1502 3.545415177 TLIIPPLPEKFIVK SNX7_HUMAN (90-103) Sorting nexin 7. (1019- 842 3.531481178 CKQPPRFPRKSTFS SOS2_HUMAN 1032) Son of sevenless protein homolog 2 (SOS-2).

80 3.496077509 DGQKPVPTQPLLK RAG1_HUMAN (70-82) V(D)J recombination activating protein 1 (RAG-1) (RING finger protein74). Dystrobrevin binding protein 1 (Dysbindin) (Hermansky-Pudlak syndrome7 protein) (My031 634 3.458612136 SKPRTVPFLPKYS DTBP1_HUMAN (34-46) protein). (657- 397 3.374368449 MKKRNAPTPPKRS ABL2_HUMAN 669) Tyrosine-protein kinase ABL2 (EC 2.7.1.112) (Tyrosine kinase ARG). (982- 1499 3.337756259 TKNAPSLPSRTCYK AKAP2_HUMAN 995) A-kinase anchor protein 2 (Protein kinase A anchoring protein 2)(PRKA2) (AKAP-2). (283- 447 3.326950266 PFFRSAPSCPQNK PERE_HUMAN 295) Eosinophil peroxidase precursor (EC 1.11.1.7) (EPO). (260- Filamin C (Gamma-filamin) (Filamin 2) (Protein FLNc) (Actin-binding-like protein) (ABP-L) 797 3.311239031 AKLKPGAPVRSKQL FLNC_HUMAN 273) (ABP-280-like protein). (371- 896 3.308182091 EFHYPPAPSKEKRK MEPE_HUMAN 384) Matrix extracellular phosphoglycoprotein precursor(Osteoblast/osteocyte factor 45) (OF45). (539- Disks large-associated protein 1 (DAP-1) (Guanylate kinase-associatedprotein) (hGKAP) 1073 3.264531733 KKTPPPVPPRTTTK DLP1_HUMAN 552) (SAP90/PSD-95-associated protein 1) (SAPAP1) (PSD-95/SAP90 binding protein 1). (1102- 539 3.199767177 QSKKPIPSKPQLK MINT_HUMAN 1114) Msx2-interacting protein (SPEN homolog) (SMART/HDAC1 associatedrepressor protein). (1328- 813 3.157538682 APHCPRLPLRKTYR GSCR1_HUMAN 1341) Glioma tumor suppressor candidate region gene 1 protein. (671- Liprin-alpha 4 (Protein tyrosine phosphatase receptor type fpolypeptide-interacting protein 1503 3.15540599 TLQPPPAPPKKIMP LIPA4_HUMAN 684) alpha 4) (PTPRF-interacting proteinalpha 4). (583- 820 3.114741453 AQNNPPVPPKPQPK ADNP_HUMAN 596) Activity-dependent neuroprotector (Activity-dependent neuroprotectiveprotein). Proteinase activated receptor 3 precursor (PAR-3) (Thrombin receptor-like 2) (Coagulation 1225 3.102158295 NLAKPTLPIKTFRG PAR3_HUMAN (29-42) factor II receptor-like 2). (256- Disks large-associated protein 3 (DAP-3) (SAP90/PSD-95-associatedprotein 3) (SAPAP3) (PSD- 1074 3.09874586 KKVPPPIPKKPLRG DLP3_HUMAN 269) 95/SAP90 binding protein 3) (Fragment).

Table S2. List of SH3-Interacting Proteins Identified by PATS*

Interacting Target SH3 Proteins Proteins Binding Site Position ABL1_HUMAN LMO6_HUMAN LLGRPFLPRRGLIF (348-361) ABL1_HUMAN M4K1_HUMAN EDTPPPLPPKPKFR (391-404) ABL1_HUMAN NBEA_HUMAN GMINPSVPIRNIRM (59-72) ABL1_HUMAN HNRPR_HUMAN RGGRGAPPPPRGR (506-518) ABL1_HUMAN PAR3_HUMAN NLAKPTLPIKTFRG (29-42) ABL1_HUMAN GPR45_HUMAN FQILPKVPERIRRR (343-356) ABL1_HUMAN CNTP1_HUMAN FVQVPGFPRRGRLA (371-384) ABL1_HUMAN SOS2_HUMAN CKQPPRFPRKSTFS (1019-1032) ABL1_HUMAN CENPA_HUMAN RSRKPEAPRRRSPS (6-19) ABL1_HUMAN DNSL2_HUMAN GERAPPLPSRRALT (140-153) ABL1_HUMAN PKD1_HUMAN PGLRPALPSRLARA (4264-4277) ABL1_HUMAN CSMD2_HUMAN MCPDPGIPERGKRL (342-355) ABL1_HUMAN DYN2_HUMAN FPAPPQIPSRPVRI (825-838) ABL1_HUMAN FLNC_HUMAN AKLKPGAPVRSKQL (260-273) ABL1_HUMAN SREC2_HUMAN SMKLPRIPLRRQKL (496-509) ABL1_HUMAN SF3B4_HUMAN PTPRPPVPPRGPLR (406-419) ABL1_HUMAN ABR_HUMAN LLNSPTIPFRIHNR (419-432) ABL1_HUMAN AKAP6_HUMAN SPTQPTLPKRGLFL (383-396) ABL1_HUMAN BAZ2A_HUMAN FTQKPGFPKRGQKR (1705-1718) ABL1_HUMAN ORC1_HUMAN EASTPPLPRRAPRT (425-438) ABL1_HUMAN MLL4_HUMAN EPGGPPAPRRRTPR (1096-1109) ABL1_HUMAN BRCA2_HUMAN NTTKPYLPSRALTR (2879-2892) ABL1_HUMAN SHAN3_HUMAN LLEKPPVPPKPKLK (472-485) ABL1_HUMAN GABR1_HUMAN FFSDPAVPVKNLKR (343-356) ABL1_HUMAN DOCK3_HUMAN GDTPPALPARTLRK (1848-1861) ABL1_HUMAN DPOD1_HUMAN PGPGPGVPPKRARG (7-20) ABL1_HUMAN CELR3_HUMAN FRCRFLPQRPGPR (282-294) ABL1_HUMAN SUV92_HUMAN CQCGPDCPNRIVQK (233-246) ABL1_HUMAN KI67_HUMAN NTSLPPLPFKRGGG (2988-3001) ABL1_HUMAN CDKL5_HUMAN KPHTPCVPNRALHR (943-956) ABL1_HUMAN NAP5_HUMAN PNFRTAPGMPSRR (210-222) ABL1_HUMAN DLP3_HUMAN KKVPPPIPKKPLRG (256-269) ABL1_HUMAN EP15_HUMAN TPTRPCPLPPGKR (777-789) ABL1_HUMAN SNX17_HUMAN ANVLPAFPPKKLFS (53-66) ABL1_HUMAN GRIP2_HUMAN QLDRPLLPRKSGSL (737-750) ABL1_HUMAN RIMS1_HUMAN PQPSPFMPRRHIHG (878-891) ABL1_HUMAN CNTFR_HUMAN VVARPVPSNPRRL (210-222) ABL1_HUMAN SPN90_HUMAN SPTPPPVPRRGTHT (240-253) ABL1_HUMAN RGS20_HUMAN RRPPPEAPRRRLDF (91-104) ABL1_HUMAN CTGE5_HUMAN FVPPPLAPVRGPLF (691-704) ABL1_HUMAN DUS15_HUMAN VADTPEVPIKKHFK (52-65) ABL1_HUMAN FETUA_HUMAN GPVVPPCPGRIRHF (352-365) ABL1_HUMAN WNK2_HUMAN GLTLPCLPWRRAAC (1349-1362) ABL1_HUMAN RPGF1_HUMAN TDTPPALPEKKRRS (450-463) ABL1_HUMAN CD2AP_HUMAN TANRPKMPGRRLPG (490-503) ABL1_HUMAN RIN3_HUMAN PAKKNLPTAPPRR (377-389) ABL1_HUMAN NXPH3_HUMAN GQPRPRVPRKRGHI (40-53) ABL1_HUMAN EFS_HUMAN IQDRPLPPPPPRL (332-344) ABL1_HUMAN ARHGB_HUMAN DKDKWLPFFPKTK (504-516) ABL1_HUMAN LRP2_HUMAN SDEKDCPTQPFRC (1300-1312) ABL1_HUMAN NEK8_HUMAN VRCRGIPRGPVRP (293-305) ABL1_HUMAN OSR5_HUMAN QSETPGAPVRRGTT (329-342) ABL1_HUMAN RTN4_HUMAN PPSTPAAPKRRGSS (169-182) ABL1_HUMAN CRSP2_HUMAN MGDDPDVPWRLLKL (237-250) ABL1_HUMAN RFRP_HUMAN SLVRRVPNLPQRF (119-131) ABL1_HUMAN SYNJ2_HUMAN PPLVPKVPPRRKKS (1326-1339) ABL1_HUMAN GASR_HUMAN TCARCCPRPPRAR (404-416) ABL1_HUMAN DLP1_HUMAN KKTPPPVPPRTTTK (539-552) ABL1_HUMAN LAP2B_HUMAN DIPRRAPKKPLTR (315-327) ABL1_HUMAN P2RX7_HUMAN QEQRLCPEYPTRR (114-126) ABL1_HUMAN FYB_HUMAN QPPVPSLPPRNIKP (413-426) ABL1_HUMAN LTBP2_HUMAN PRTRAAPALPRLG (134-146) ABL1_HUMAN GT2D1_HUMAN EGLPPGIPFRKPCT (744-757) ABL1_HUMAN DYN1_HUMAN FGPPPQVPSRPNRA (826-839) ABL1_HUMAN DDEF1_HUMAN PRVLPKLPQKVALR (718-731) ABL1_HUMAN G3B2_HUMAN PRERPGFPPRGPRP (296-309) ABL1_HUMAN DNL3_HUMAN RSLKPVLPHKVAHF (441-454) ABL1_HUMAN DLP2_HUMAN KKTPPPVPPRTTSK (575-588) ABL1_HUMAN DLP4_HUMAN KKTPPPVPPRTTSK (558-571) ABL1_HUMAN TSKS_HUMAN SQLVPEAPRRVTSR (29-42) ABL1_HUMAN VPREB_HUMAN KSQGPQVPPRFSGS (77-90) ABL1_HUMAN BRPF3_HUMAN NSEQPQFPGKSKKP (89-102) ABL1_HUMAN P2BA_HUMAN RVVKAVPFPPSHR (16-28) ABL1_HUMAN CUL7_HUMAN GIQLPQVPGRVLFS (239-252) ABL1_HUMAN APOL5_HUMAN HQPAPPAPARKGRQ (413-426) ABL1_HUMAN UHMK1_HUMAN IHFSPNVPSRCLLL (98-111) ABL1_HUMAN FLNA_HUMAN AKLKPGAPLRPKLN (267-280) ABL1_HUMAN PTN4_HUMAN DGKPPALPPKQSKK (459-472) ABL1_HUMAN PCDA7_HUMAN CSLTPRVPFKLVST (387-400) ABL1_HUMAN EFNA5_HUMAN AAQTPRIPSRLLAI (204-217) ABL1_HUMAN DCE1_HUMAN QSLRGVPDSPQRR (520-532) CRK_HUMAN STF1_HUMAN GYLYPAFPGRAIKS (182-195) CRK_HUMAN FLNC_HUMAN AKLKPGAPVRSKQL (260-273) CRK_HUMAN CNTP1_HUMAN FVQVPGFPRRGRLA (371-384) CRK_HUMAN MLL4_HUMAN EPGGPPAPRRRTPR (1096-1109) CRK_HUMAN DOCK3_HUMAN GDTPPALPARTLRK (1848-1861) CRK_HUMAN M4K1_HUMAN EDTPPPLPPKPKFR (391-404) CRK_HUMAN HNRPR_HUMAN RGGRGAPPPPRGR (506-518) CRK_HUMAN TAL1_HUMAN ALAAPAAPGRALLY (125-138) CRK_HUMAN ARHGB_HUMAN DKDKWLPFFPKTK (504-516) CRK_HUMAN FLNA_HUMAN AKLKPGAPLRPKLN (267-280) CRK_HUMAN PCDA7_HUMAN CSLTPRVPFKLVST (387-400) CRK_HUMAN PCDA1_HUMAN CSLMPHVPFKLVST (387-400) CRK_HUMAN PCDA5_HUMAN CSLMPHVPFKLVST (386-399) CRK_HUMAN PCDA8_HUMAN CSLMPHVPFKLVST (387-400) CRK_HUMAN KIF22_HUMAN NQGLPRVPYRDSKL (312-325) CRK_HUMAN BMX_HUMAN KHRVPTFPDRVLKI (160-173) CRK_HUMAN DYN2_HUMAN FPAPPQIPSRPVRI (825-838) CRK_HUMAN NMDE4_HUMAN EDESPPAPARWPRS (1051-1064) CRK_HUMAN FASTK_HUMAN GYRGPRLPRRQQVP (371-384) CRK_HUMAN RGS20_HUMAN RRPPPEAPRRRLDF (91-104) CRK_HUMAN CAD11_HUMAN NDNPPKFPQRLYQM (262-275) CRK_HUMAN GPIX_HUMAN LTALPALPARTRHL (43-56) CRK_HUMAN PCDA2_HUMAN CSLTPHVPFKLVST (387-400) CRK_HUMAN PCDA3_HUMAN CSLTPHVPFKLVST (387-400) CRK_HUMAN PCDA6_HUMAN CSLTPHVPFKLVST (387-400) CRK_HUMAN PCDAA_HUMAN CSLTPHVPFKLVST (386-399) CRK_HUMAN PCDAB_HUMAN CSLTPHVPFKLVST (386-399) CRK_HUMAN GABR1_HUMAN FFSDPAVPVKNLKR (343-356) CRK_HUMAN LMO6_HUMAN LLGRPFLPRRGLIF (348-361) CRK_HUMAN BRD4_HUMAN EPKTPVAPKKDLKI (1183-1196) CRK_HUMAN CNTFR_HUMAN VVARPVPSNPRRL (210-222) CRK_HUMAN DLP3_HUMAN KKVPPPIPKKPLRG (256-269) CRK_HUMAN GCF_HUMAN SGRAPGVPPKRLPA (111-124) CRK_HUMAN DUS15_HUMAN VADTPEVPIKKHFK (52-65) CRK_HUMAN SNX17_HUMAN ANVLPAFPPKKLFS (53-66) CRK_HUMAN RPGF1_HUMAN TDTPPALPEKKRRS (450-463) CRK_HUMAN SHAN3_HUMAN LLEKPPVPPKPKLK (472-485) CRK_HUMAN RIN3_HUMAN QVPAPPLPAKKNLP (370-383) CRK_HUMAN NXPH3_HUMAN GQPRPRVPRKRGHI (40-53) CRK_HUMAN AKAP6_HUMAN SPTQPTLPKRGLFL (383-396) CRK_HUMAN FLNC_HUMAN AKLKPGAPVRSKQL (260-273) CRK_HUMAN M4K1_HUMAN EDTPPPLPPKPKFR (391-404) CRK_HUMAN MINT_HUMAN QSKKPIPSKPQLK (1102-1114) CRK_HUMAN ABL2_HUMAN LPRLPILPSKTRTL (569-582) CRK_HUMAN AKAP2_HUMAN TKNAPSLPSRTCYK (982-995) CRK_HUMAN ARHGB_HUMAN DKDKWLPFFPKTK (504-516) CRK_HUMAN ABL1_HUMAN KKKKTAPTPPKRS (606-618) CRK_HUMAN DDEF2_HUMAN TTSAPPLPPRNVGK (781-794) CRK_HUMAN FCG2B_HUMAN VAGTPAAPPKAVLK (40-53) CRK_HUMAN FCG2C_HUMAN VAGTPAAPPKAVLK (40-53) CRK_HUMAN DDEF1_HUMAN TTEAPPLPPRNAGK (614-627) CRK_HUMAN APOL5_HUMAN HQPAPPAPARKGRQ (413-426) CRK_HUMAN ID4_HUMAN RLRRLVPTIPPNK (74-86) CRK_HUMAN DOCK1_HUMAN FALEPLLPKKMHSR (1667-1680) CRK_HUMAN RRAS_HUMAN PPSPPSAPRKKGGG (199-212) CRK_HUMAN SOS1_HUMAN PKPLPRFPKKYSYP (1021-1034) CRK_HUMAN RGS7_HUMAN NVGRNIPIFPCHK (470-482) CRK_HUMAN SOS2_HUMAN PLIPPPLPPRKKFD (1143-1156) CRK_HUMAN DLP1_HUMAN KKTPPPVPPRTTTK (539-552) CRK_HUMAN EFS_HUMAN NRVKLLPAGPAPK (61-73) CRK_HUMAN SHAN2_HUMAN MVDKPPVPPKPKMK (944-957) CRK_HUMAN CSMD2_HUMAN MCPDPGIPERGKRL (342-355) CRK_HUMAN DNL3_HUMAN RSLKPVLPHKVAHF (441-454) CRK_HUMAN FLNA_HUMAN AKLKPGAPLRPKLN (267-280) CRK_HUMAN DLP2_HUMAN KKTPPPVPPRTTSK (575-588) CRK_HUMAN DLP4_HUMAN KKTPPPVPPRTTSK (558-571) CRK_HUMAN FLNB_HUMAN AKLKPGAPLKPKLN (240-253) CRK_HUMAN C1QR1_HUMAN KCLDPSLPLKGFSW (103-116) CRK_HUMAN GHR_HUMAN DAKKCIPVAPHIK (539-551) CRK_HUMAN SUV92_HUMAN CQCGPDCPNRIVQK (233-246) CRK_HUMAN CELR2_HUMAN NGTVPGCPAKKNVC (1565-1578) CRK_HUMAN GRIP2_HUMAN QLDRPLLPRKSGSL (737-750) CRK_HUMAN RAG1_HUMAN DGQKPVPTQPLLK (70-82) CRK_HUMAN ASPP2_HUMAN AKVPPPVPTKPKQI (496-509) CRK_HUMAN PAR3_HUMAN NLAKPTLPIKTFRG (29-42) CRK_HUMAN TPX2_HUMAN LEGGPILPKKPPVK (412-425) CRK_HUMAN DTX1_HUMAN PPGVPALPVKNLNG (312-325) SRC_HUMAN DDEF1_HUMAN PRVLPKLPQKVALR (718-731) SRC_HUMAN DOCK3_HUMAN GDTPPALPARTLRK (1848-1861) SRC_HUMAN ABL1_HUMAN KKKKTAPTPPKRS (606-618) SRC_HUMAN DLP2_HUMAN KKTPPPVPPRTTSK (575-588) SRC_HUMAN DLP4_HUMAN KKTPPPVPPRTTSK (558-571) SRC_HUMAN SNX17_HUMAN ANVLPAFPPKKLFS (53-66) SRC_HUMAN DLP3_HUMAN KKVPPPIPKKPLRG (256-269) SRC_HUMAN RTN4_HUMAN PPSTPAAPKRRGSS (169-182) SRC_HUMAN DDEF2_HUMAN TTSAPPLPPRNVGK (781-794) SRC_HUMAN DUS15_HUMAN VADTPEVPIKKHFK (52-65) SRC_HUMAN FCG2B_HUMAN VAGTPAAPPKAVLK (40-53) SRC_HUMAN FCG2C_HUMAN VAGTPAAPPKAVLK (40-53) SRC_HUMAN RL13_HUMAN QLTGPVMPVRNVYK (148-161) SRC_HUMAN DLP1_HUMAN KKTPPPVPPRTTTK (539-552) SRC_HUMAN NXPH3_HUMAN GQPRPRVPRKRGHI (40-53) SRC_HUMAN RPGF1_HUMAN NSPPPALPPKKRQS (280-293) SRC_HUMAN SHAN2_HUMAN TARKKAPPPPKRA (139-151) SRC_HUMAN DNL3_HUMAN RSLKPVLPHKVAHF (441-454) SRC_HUMAN RIN3_HUMAN QVPAPPLPAKKNLP (370-383) SRC_HUMAN EFS_HUMAN NRVKLLPAGPAPK (61-73) SRC_HUMAN ABL2_HUMAN MKKRNAPTPPKRS (657-669) SRC_HUMAN SHAN3_HUMAN LLEKPPVPPKPKLK (472-485) SRC_HUMAN APOL5_HUMAN HQPAPPAPARKGRQ (413-426) SRC_HUMAN SOS2_HUMAN PLIPPPLPPRKKFD (1143-1156) SRC_HUMAN M4K1_HUMAN EDTPPPLPPKPKFR (391-404) SRC_HUMAN PAR3_HUMAN NLAKPTLPIKTFRG (29-42) SRC_HUMAN PARC_HUMAN GIHLPQIPGKLLFS (245-258) SRC_HUMAN DOCK1_HUMAN FALEPLLPKKMHSR (1667-1680) SRC_HUMAN CELR2_HUMAN NGTVPGCPAKKNVC (1565-1578) SRC_HUMAN SOS1_HUMAN PKPLPRFPKKYSYP (1021-1034) SRC_HUMAN AKAP6_HUMAN SPTQPTLPKRGLFL (383-396) SRC_HUMAN ZP2_HUMAN TLTIPEFPGKLKSV (274-287) SRC_HUMAN DPOD1_HUMAN PGPGPGVPPKRARG (7-20) SRC_HUMAN DAG1_HUMAN ANKKPPLPKRVRRQ (300-313) SRC_HUMAN KI67_HUMAN NTSLPPLPFKRGGG (2988-3001) SRC_HUMAN CD97_HUMAN ILAKSLPKGPFTY (340-352) SRC_HUMAN LMO6_HUMAN LLGRPFLPRRGLIF (348-361) SRC_HUMAN ID4_HUMAN RLRRLVPTIPPNK (74-86) SRC_HUMAN AKAP2_HUMAN TKNAPSLPSRTCYK (982-995) SRC_HUMAN RRAS_HUMAN PPSPPSAPRKKGGG (199-212) FYN_HUMAN LMO6_HUMAN LLGRPFLPRRGLIF (348-361) FYN_HUMAN DOCK3_HUMAN GDTPPALPARTLRK (1848-1861) FYN_HUMAN ORC1_HUMAN EASTPPLPRRAPRT (425-438) FYN_HUMAN AKAP6_HUMAN SPTQPTLPKRGLFL (383-396) FYN_HUMAN CENPA_HUMAN RSRKPEAPRRRSPS (6-19) FYN_HUMAN TRAIP_HUMAN VMIRPLPVKPKTK (433-445) FYN_HUMAN M4K1_HUMAN EDTPPPLPPKPKFR (391-404) FYN_HUMAN RPGF1_HUMAN TDTPPALPEKKRRS (450-463) FYN_HUMAN DDEF2_HUMAN TTSAPPLPPRNVGK (781-794) FYN_HUMAN DUS15_HUMAN VADTPEVPIKKHFK (52-65) FYN_HUMAN SREC2_HUMAN SMKLPRIPLRRQKL (496-509) FYN_HUMAN SOS2_HUMAN CKQPPRFPRKSTFS (1019-1032) FYN_HUMAN GPR45_HUMAN FQILPKVPERIRRR (343-356) FYN_HUMAN SYNJ2_HUMAN PPLVPKVPPRRKKS (1326-1339) FYN_HUMAN NBEA_HUMAN GMINPSVPIRNIRM (59-72) FYN_HUMAN DCE1_HUMAN QSLRGVPDSPQRR (520-532) FYN_HUMAN RIMS1_HUMAN RRGRQLPQVPVRS (1266-1278) FYN_HUMAN CSMD2_HUMAN MCPDPGIPERGKRL (342-355) FYN_HUMAN RIN3_HUMAN PAKKNLPTAPPRR (377-389) FYN_HUMAN PAR3_HUMAN NLAKPTLPIKTFRG (29-42) FYN_HUMAN DLP2_HUMAN KKTPPPVPPRTTSK (575-588) FYN_HUMAN DLP4_HUMAN KKTPPPVPPRTTSK (558-571) FYN_HUMAN LAP2B_HUMAN DIPRRAPKKPLTR (315-327) FYN_HUMAN GABR1_HUMAN FFSDPAVPVKNLKR (343-356) FYN_HUMAN HNRPR_HUMAN RGGRGAPPPPRGR (506-518) FYN_HUMAN EP15_HUMAN TPTRPCPLPPGKR (777-789) FYN_HUMAN RFRP_HUMAN SLVRRVPNLPQRF (119-131) FYN_HUMAN CD2AP_HUMAN TANRPKMPGRRLPG (490-503) FYN_HUMAN MLL4_HUMAN EPGGPPAPRRRTPR (1096-1109) FYN_HUMAN APOL5_HUMAN HQPAPPAPARKGRQ (413-426) FYN_HUMAN DLP3_HUMAN KKVPPPIPKKPLRG (256-269) FYN_HUMAN SHAN3_HUMAN LLEKPPVPPKPKLK (472-485) FYN_HUMAN ARHGB_HUMAN DKDKWLPFFPKTK (504-516) FYN_HUMAN FLNC_HUMAN AKLKPGAPVRSKQL (260-273) FYN_HUMAN CNTFR_HUMAN VVARPVPSNPRRL (210-222) FYN_HUMAN RIMS2_HUMAN RRGRQLPQLPPKG (1157-1169) FYN_HUMAN CNTP1_HUMAN FVQVPGFPRRGRLA (371-384) FYN_HUMAN DNSL2_HUMAN GERAPPLPSRRALT (140-153) FYN_HUMAN ABL1_HUMAN KKKKTAPTPPKRS (606-618) FYN_HUMAN ABL2_HUMAN MKKRNAPTPPKRS (657-669) FYN_HUMAN WNK2_HUMAN GLTLPCLPWRRAAC (1349-1362) FYN_HUMAN GUC2D_HUMAN RLPRALPRLPLLL (28-40) FYN_HUMAN RTN4_HUMAN PPSTPAAPKRRGSS (169-182) FYN_HUMAN SF3B4_HUMAN PTPRPPVPPRGPLR (406-419) FYN_HUMAN GCF_HUMAN SGRAPGVPPKRLPA (111-124) FYN_HUMAN PPR3D_HUMAN RRARSLPSSPERR (70-82) FYN_HUMAN PKD1_HUMAN PGLRPALPSRLARA (4264-4277) FYN_HUMAN BAZ2A_HUMAN FTQKPGFPKRGQKR (1705-1718) FYN_HUMAN KI67_HUMAN NTSLPPLPFKRGGG (2988-3001) FYN_HUMAN CCR10_HUMAN QPRRGCPRRPRLS (335-347) FYN_HUMAN DPOD1_HUMAN PGPGPGVPPKRARG (7-20) FYN_HUMAN FLNA_HUMAN AKLKPGAPLRPKLN (267-280) FYN_HUMAN DDEF1_HUMAN TTEAPPLPPRNAGK (614-627) FYN_HUMAN DLP1_HUMAN KKTPPPVPPRTTTK (539-552) FYN_HUMAN TSKS_HUMAN SQLVPEAPRRVTSR (29-42) FYN_HUMAN CRSP2_HUMAN MGDDPDVPWRLLKL (237-250) FYN_HUMAN WASIP_HUMAN NDETPRLPQRNLSL (328-341) FYN_HUMAN BCA1_HUMAN IQSRPLPSPPKFT (632-644) FYN_HUMAN MYPT1_HUMAN AYVAPTIPRRLAST (495-508) FYN_HUMAN CAF1A_HUMAN CKDRPAFPVKKLIQ (3-16) FYN_HUMAN PTN4_HUMAN DGKPPALPPKQSKK (459-472) FYN_HUMAN CDKL5_HUMAN KPHTPCVPNRALHR (943-956) FYN_HUMAN RRAS_HUMAN PPSPPSAPRKKGGG (199-212) FYN_HUMAN SPN90_HUMAN SPTPPPVPRRGTHT (240-253) FYN_HUMAN HDAC6_HUMAN DSHHPEVPQRILRI (497-510) FYN_HUMAN BRPF3_HUMAN NSEQPQFPGKSKKP (89-102) FYN_HUMAN IL3RA_HUMAN VMQRLFPRIPHMK (330-342) FYN_HUMAN DAB2_HUMAN QQAAPKAPSKKEKK (16-29) FYN_HUMAN M4K5_HUMAN SAQAPQLPRKNDKR (463-476) FYN_HUMAN LTBP2_HUMAN PRTRAAPALPRLG (134-146) FYN_HUMAN STF1_HUMAN GYLYPAFPGRAIKS (182-195) FYN_HUMAN DOCK1_HUMAN FALEPLLPKKMHSR (1667-1680) FYN_HUMAN GPR63_HUMAN KSFKFLPQLPGHT (388-400) FYN_HUMAN BRCA2_HUMAN NTTKPYLPSRALTR (2879-2892) FYN_HUMAN TDRD1_HUMAN SLMRLCPIIPKLL (183-195) FYN_HUMAN NAP5_HUMAN PNFRTAPGMPSRR (210-222) FYN_HUMAN DAG1_HUMAN ANKKPPLPKRVRRQ (300-313) FYN_HUMAN SUV92_HUMAN CQCGPDCPNRIVQK (233-246) FYN_HUMAN FCG2B_HUMAN VAGTPAAPPKAVLK (40-53) FYN_HUMAN FCG2C_HUMAN VAGTPAAPPKAVLK (40-53) FYN_HUMAN NEK8_HUMAN VRCRGIPRGPVRP (293-305) FYN_HUMAN CELR3_HUMAN FRCRFLPQRPGPR (282-294) FYN_HUMAN FYB_HUMAN QPPVPSLPPRNIKP (413-426) FYN_HUMAN GASR_HUMAN TCARCCPRPPRAR (404-416) FYN_HUMAN SOS1_HUMAN PKPLPRFPKKYSYP (1021-1034) FYN_HUMAN MOS_HUMAN LPRAPRLPRRLAWC (40-53) FYN_HUMAN SNX17_HUMAN ANVLPAFPPKKLFS (53-66) GRB2_HUMAN BAZ2A_HUMAN FTQKPGFPKRGQKR (1705-1718) GRB2_HUMAN M4K1_HUMAN EDTPPPLPPKPKFR (391-404) GRB2_HUMAN SHAN3_HUMAN LLEKPPVPPKPKLK (472-485) GRB2_HUMAN GABR1_HUMAN FFSDPAVPVKNLKR (343-356) GRB2_HUMAN EP15_HUMAN TPTRPCPLPPGKR (777-789) GRB2_HUMAN DLP1_HUMAN KKTPPPVPPRTTTK (539-552) GRB2_HUMAN SYNJ2_HUMAN PPLVPKVPPRRKKS (1326-1339) GRB2_HUMAN HNRPR_HUMAN RGGRGAPPPPRGR (506-518) GRB2_HUMAN DLP2_HUMAN KKTPPPVPPRTTSK (575-588) GRB2_HUMAN DLP4_HUMAN KKTPPPVPPRTTSK (558-571) GRB2_HUMAN PTN4_HUMAN DGKPPALPPKQSKK (459-472) GRB2_HUMAN DOCK3_HUMAN GDTPPALPARTLRK (1848-1861) GRB2_HUMAN DUS15_HUMAN VADTPEVPIKKHFK (52-65) GRB2_HUMAN SUV92_HUMAN CQCGPDCPNRIVQK (233-246) GRB2_HUMAN SREC2_HUMAN SMKLPRIPLRRQKL (496-509) GRB2_HUMAN M4K3_HUMAN AKIPPPLPPKPKSI (429-442) GRB2_HUMAN ABL1_HUMAN KKKKTAPTPPKRS (606-618) GRB2_HUMAN FLNC_HUMAN AKLKPGAPVRSKQL (260-273) GRB2_HUMAN FCG2B_HUMAN VAGTPAAPPKAVLK (40-53) GRB2_HUMAN FCG2C_HUMAN VAGTPAAPPKAVLK (40-53) GRB2_HUMAN BRPF3_HUMAN NSEQPQFPGKSKKP (89-102) GRB2_HUMAN DDEF2_HUMAN TTSAPPLPPRNVGK (781-794) GRB2_HUMAN DAB2_HUMAN QQAAPKAPSKKEKK (16-29) GRB2_HUMAN PAR3_HUMAN NLAKPTLPIKTFRG (29-42) GRB2_HUMAN ID4_HUMAN RLRRLVPTIPPNK (74-86) GRB2_HUMAN RIMS2_HUMAN RRGRQLPQLPPKG (1157-1169) GRB2_HUMAN SOS1_HUMAN RNPKPLPRFPKKY (1019-1031) GRB2_HUMAN GPR45_HUMAN FQILPKVPERIRRR (343-356) GRB2_HUMAN ECT2_HUMAN NIVKDLPFEPSKK (285-297) GRB2_HUMAN DDEF1_HUMAN PRVLPKLPQKVALR (718-731) GRB2_HUMAN DLP3_HUMAN KKVPPPIPKKPLRG (256-269) GRB2_HUMAN TRAIP_HUMAN VMIRPLPVKPKTK (433-445) GRB2_HUMAN FLNB_HUMAN AKLKPGAPLKPKLN (240-253) GRB2_HUMAN ELK3_HUMAN KTKSPSLPPKAKKP (281-294) GRB2_HUMAN LMO6_HUMAN LLGRPFLPRRGLIF (348-361) GRB2_HUMAN RL13_HUMAN QLTGPVMPVRNVYK (148-161) GRB2_HUMAN MYLK_HUMAN VKKKPAPKTPPKA (1222-1234) GRB2_HUMAN ABL2_HUMAN MKKRNAPTPPKRS (657-669) GRB2_HUMAN SHAN2_HUMAN MVDKPPVPPKPKMK (944-957) GRB2_HUMAN P2RX7_HUMAN QEQRLCPEYPTRR (114-126) GRB2_HUMAN HMG17_HUMAN LSAKPAPPKPEPK (27-39) GRB2_HUMAN HMGN3_HUMAN LSAKPAPPKPEPK (30-42) GRB2_HUMAN FLNA_HUMAN AKLKPGAPLRPKLN (267-280) GRB2_HUMAN DOCK1_HUMAN FALEPLLPKKMHSR (1667-1680) GRB2_HUMAN TPX2_HUMAN ISQEPFVPKKEKKS (633-646) GRB2_HUMAN RIN3_HUMAN PAKKNLPTAPPRR (377-389) GRB2_HUMAN CNTFR_HUMAN VVARPVPSNPRRL (210-222) GRB2_HUMAN CELR3_HUMAN FRCRFLPQRPGPR (282-294) GRB2_HUMAN CNTP1_HUMAN FVQVPGFPRRGRLA (371-384) GRB2_HUMAN TSKS_HUMAN SQLVPEAPRRVTSR (29-42) GRB2_HUMAN PLCG2_HUMAN SEINPSMPQRTVKA (763-776) GRB2_HUMAN GASR_HUMAN TCARCCPRPPRAR (404-416) GRB2_HUMAN DAG1_HUMAN ANKKPPLPKRVRRQ (300-313) GRB2_HUMAN ARHGB_HUMAN DKDKWLPFFPKTK (504-516) GRB2_HUMAN BRD4_HUMAN EPKTPVAPKKDLKI (1183-1196) GRB2_HUMAN MINT_HUMAN QSKKPIPSKPQLK (1102-1114) GRB2_HUMAN IL3RA_HUMAN VMQRLFPRIPHMK (330-342) GRB2_HUMAN NAP5_HUMAN PNFRTAPGMPSRR (210-222) GRB2_HUMAN MLL4_HUMAN EPGGPPAPRRRTPR (1096-1109) GRB2_HUMAN EFS_HUMAN NRVKLLPAGPAPK (61-73) GRB2_HUMAN SOS2_HUMAN CKQPPRFPRKSTFS (1019-1032) GRB2_HUMAN DCE1_HUMAN QSLRGVPDSPQRR (520-532) GRB2_HUMAN M4K5_HUMAN SAQAPQLPRKNDKR (463-476) GRB2_HUMAN LAP2B_HUMAN DIPRRAPKKPLTR (315-327) GRB2_HUMAN P3C2B_HUMAN LNQLPPCPSRMQPK (555-568) GRB2_HUMAN RYR1_HUMAN PPKKQAPPSPPPK (4521-4533) GRB2_HUMAN AKAP2_HUMAN TKNAPSLPSRTCYK (982-995) GRB2_HUMAN SF3B4_HUMAN PTPRPPVPPRGPLR (406-419) GRB2_HUMAN SNX17_HUMAN ANVLPAFPPKKLFS (53-66) GRB2_HUMAN NEK8_HUMAN VRCRGIPRGPVRP (293-305) GRB2_HUMAN GRIP2_HUMAN QLDRPLLPRKSGSL (737-750) GRB2_HUMAN DYN1_HUMAN TPQRRAPAVPPAR (780-792) GRB2_HUMAN CDKL5_HUMAN KPHTPCVPNRALHR (943-956) GRB2_HUMAN AKAP6_HUMAN SPTQPTLPKRGLFL (383-396) GRB2_HUMAN MYRIP_HUMAN QQRRKLPAPPVKA (798-810) GRB2_HUMAN P2BA_HUMAN RVVKAVPFPPSHR (16-28) GRB2_HUMAN IL5RA_HUMAN LWIKLFPPIPAPK (367-379) GRB2_HUMAN STF1_HUMAN GYLYPAFPGRAIKS (182-195) GRB2_HUMAN P2BB_HUMAN RVVKAVPFPPTHR (25-37) GRB2_HUMAN WASL_HUMAN ARGRGAPPPPPSR (304-316) GRB2_HUMAN CRSP2_HUMAN MGDDPDVPWRLLKL (237-250) GRB2_HUMAN RAGE_HUMAN LKCKGAPKKPPQR (36-48) GRB2_HUMAN GHR_HUMAN DAKKCIPVAPHIK (539-551) GRB2_HUMAN IL2RG_HUMAN WLERTMPRIPTLK (282-294) GRB2_HUMAN DPOD1_HUMAN PGPGPGVPPKRARG (7-20) GRB2_HUMAN RRAS_HUMAN PPSPPSAPRKKGGG (199-212) GRB2_HUMAN APOL5_HUMAN HQPAPPAPARKGRQ (413-426) GRB2_HUMAN FOXH1_HUMAN RCFRKVPKDPAKP (89-101) GRB2_HUMAN BAT8_HUMAN HATKSFPSSPSKG (111-123) GRB2_HUMAN BRCA2_HUMAN NTTKPYLPSRALTR (2879-2892) GRB2_HUMAN RIMS1_HUMAN RRGRQLPQVPVRS (1266-1278) GRB2_HUMAN CCR10_HUMAN QPRRGCPRRPRLS (335-347) GRB2_HUMAN JAK2_HUMAN QFTKCCPPKPKDK (494-506) GRB2_HUMAN M4K1_HUMAN EDTPPPLPPKPKFR (391-404) GRB2_HUMAN ABL1_HUMAN KKKKTAPTPPKRS (606-618) GRB2_HUMAN FLNB_HUMAN AKLKPGAPLKPKLN (240-253) GRB2_HUMAN DOCK3_HUMAN GDTPPALPARTLRK (1848-1861) GRB2_HUMAN DLP3_HUMAN KKVPPPIPKKPLRG (256-269) GRB2_HUMAN FLNA_HUMAN AKLKPGAPLRPKLN (267-280) GRB2_HUMAN DUS15_HUMAN VADTPEVPIKKHFK (52-65) GRB2_HUMAN FLNC_HUMAN AKLKPGAPVRSKQL (260-273) GRB2_HUMAN RRAS_HUMAN PPSPPSAPRKKGGG (199-212) GRB2_HUMAN SOS2_HUMAN CKQPPRFPRKSTFS (1019-1032) GRB2_HUMAN NXPH3_HUMAN GQPRPRVPRKRGHI (40-53) GRB2_HUMAN SHAN2_HUMAN MVDKPPVPPKPKMK (944-957) GRB2_HUMAN DLP1_HUMAN KKTPPPVPPRTTTK (539-552) GRB2_HUMAN SHAN3_HUMAN LLEKPPVPPKPKLK (472-485) GRB2_HUMAN ID4_HUMAN RLRRLVPTIPPNK (74-86) GRB2_HUMAN TLR9_HUMAN QAAVPGLPSRVKCG (778-791) GRB2_HUMAN DLP2_HUMAN KKTPPPVPPRTTSK (575-588) GRB2_HUMAN DLP4_HUMAN KKTPPPVPPRTTSK (558-571) GRB2_HUMAN EFS_HUMAN NRVKLLPAGPAPK (61-73) GRB2_HUMAN RPGF1_HUMAN NSPPPALPPKKRQS (280-293) GRB2_HUMAN ENPP3_HUMAN PYLTPDLPKRLHYA (438-451) GRB2_HUMAN PTPRK_HUMAN FWNPPLAPRKGYNI (696-709) GRB2_HUMAN APOL5_HUMAN HQPAPPAPARKGRQ (413-426) GRB2_HUMAN RIN3_HUMAN PAKKNLPTAPPRR (377-389) GRB2_HUMAN HNRPR_HUMAN RGGRGAPPPPRGR (506-518) GRB2_HUMAN SUV92_HUMAN CQCGPDCPNRIVQK (233-246) GRB2_HUMAN ARHGB_HUMAN DKDKWLPFFPKTK (504-516) GRB2_HUMAN AKAP2_HUMAN TKNAPSLPSRTCYK (982-995) GRB2_HUMAN SOS1_HUMAN PKPLPRFPKKYSYP (1021-1034) GRB2_HUMAN DDEF1_HUMAN PRVLPKLPQKVALR (718-731) GRB2_HUMAN PTN13_HUMAN RVKKLFPGQPAAE (1532-1544) GRB2_HUMAN ABL2_HUMAN MKKRNAPTPPKRS (657-669) GRB2_HUMAN BAZ2A_HUMAN FTQKPGFPKRGQKR (1705-1718) GRB2_HUMAN TRAIP_HUMAN VMIRPLPVKPKTK (433-445) GRB2_HUMAN PAR3_HUMAN NLAKPTLPIKTFRG (29-42) GRB2_HUMAN TAL2_HUMAN KLRKLIPTHPPDK (24-36) GRB2_HUMAN MYPC2_HUMAN VNGKELPDKPTIK (75-87) NCK1_HUMAN SHAN3_HUMAN LLEKPPVPPKPKLK (472-485) NCK1_HUMAN SOS2_HUMAN PLIPPPLPPRKKFD (1143-1156) NCK1_HUMAN SOS1_HUMAN PKPLPRFPKKYSYP (1021-1034) NCK1_HUMAN BRPF3_HUMAN NSEQPQFPGKSKKP (89-102) NCK1_HUMAN SYNJ2_HUMAN PPLVPKVPPRRKKS (1326-1339) NCK1_HUMAN DAB2_HUMAN QQAAPKAPSKKEKK (16-29) NCK1_HUMAN PTN4_HUMAN DGKPPALPPKQSKK (459-472) NCK1_HUMAN SNX17_HUMAN ANVLPAFPPKKLFS (53-66) NCK1_HUMAN MINT_HUMAN QSKKPIPSKPQLK (1102-1114) NCK1_HUMAN ELK3_HUMAN KTKSPSLPPKAKKP (281-294) NCK1_HUMAN DUS15_HUMAN VADTPEVPIKKHFK (52-65) NCK1_HUMAN JAK2_HUMAN QFTKCCPPKPKDK (494-506) NCK1_HUMAN GHR_HUMAN DAKKCIPVAPHIK (539-551) NCK1_HUMAN DOCK3_HUMAN GDTPPALPARTLRK (1848-1861) NCK1_HUMAN ABL2_HUMAN MKKRNAPTPPKRS (657-669) egulation of transcription from NCK1_HUMAN BRCA1_HUMAN YRIPPLFPIKSFVK RNA polyme...; NCK1_HUMAN RL13_HUMAN QLTGPVMPVRNVYK (148-161) NCK1_HUMAN FOXH1_HUMAN RCFRKVPKDPAKP (89-101) NCK1_HUMAN FLNB_HUMAN AKLKPGAPLKPKLN (240-253) NCK1_HUMAN TRAIP_HUMAN VMIRPLPVKPKTK (433-445) NCK1_HUMAN H14_HUMAN AKPKKAPKSPAKA (178-190) NCK1_HUMAN BRD4_HUMAN NAPPPGAPDKDKQK (1168-1181) NCK1_HUMAN DDEF2_HUMAN TTSAPPLPPRNVGK (781-794) NCK1_HUMAN FYB_HUMAN QPPVPSLPPRNIKP (413-426) NCK1_HUMAN ECT2_HUMAN NIVKDLPFEPSKK (285-297) NCK1_HUMAN CELR2_HUMAN NGTVPGCPAKKNVC (1565-1578) NCK1_HUMAN SHAN2_HUMAN MVDKPPVPPKPKMK (944-957) NCK1_HUMAN RAG1_HUMAN DGQKPVPTQPLLK (70-82) NCK1_HUMAN DTBP1_HUMAN SKPRTVPFLPKYS (34-46) NCK1_HUMAN RPGF1_HUMAN PEKPPPLPEKKNKH (537-550) NCK1_HUMAN SUV92_HUMAN CQCGPDCPNRIVQK (233-246) NCK1_HUMAN SASH1_HUMAN PSQPPPVPAKKSRE (981-994) NCK1_HUMAN P3C2B_HUMAN LNQLPPCPSRMQPK (555-568) NCK1_HUMAN CAF1A_HUMAN CKDRPAFPVKKLIQ (3-16) NCK1_HUMAN ABCG5_HUMAN LKTLPMVPFKTKDS (348-361) NCK1_HUMAN ID4_HUMAN RLRRLVPTIPPNK (74-86) NCK1_HUMAN ARHGB_HUMAN DKDKWLPFFPKTK (504-516) NCK1_HUMAN AKAP2_HUMAN TKNAPSLPSRTCYK (982-995) NCK1_HUMAN RRAS_HUMAN PPSPPSAPRKKGGG (199-212) NCK1_HUMAN MAP4_HUMAN PPNKELPPSPEKK (688-700) NCK1_HUMAN TPX2_HUMAN ISQEPFVPKKEKKS (633-646) NCK1_HUMAN DDEF1_HUMAN TTEAPPLPPRNAGK (614-627) NCK1_HUMAN PK3CD_HUMAN RAKPPPIPAKKPSS (300-313) NCK1_HUMAN DLP2_HUMAN KKTPPPVPPRTTSK (575-588) NCK1_HUMAN DLP4_HUMAN KKTPPPVPPRTTSK (558-571) NCK1_HUMAN FCG2B_HUMAN VAGTPAAPPKAVLK (40-53) NCK1_HUMAN FCG2C_HUMAN VAGTPAAPPKAVLK (40-53) NCK1_HUMAN GABR1_HUMAN FFSDPAVPVKNLKR (343-356) NCK1_HUMAN BAZ2A_HUMAN FTQKPGFPKRGQKR (1705-1718) NCK1_HUMAN DAG1_HUMAN ANKKPPLPKRVRRQ (300-313) NCK1_HUMAN DLP2_HUMAN KKTPPPVPPRTTSK (575-588) NCK1_HUMAN DLP4_HUMAN KKTPPPVPPRTTSK (558-571) NCK1_HUMAN DLP1_HUMAN KKTPPPVPPRTTTK (539-552) NCK1_HUMAN DLP3_HUMAN KKVPPPIPKKPLRG (256-269) NCK1_HUMAN SREC2_HUMAN SMKLPRIPLRRQKL (496-509) NCK1_HUMAN SHAN3_HUMAN LLEKPPVPPKPKLK (472-485) NCK1_HUMAN SNX17_HUMAN ANVLPAFPPKKLFS (53-66) NCK1_HUMAN M4K1_HUMAN EDTPPPLPPKPKFR (391-404) NCK1_HUMAN RIMS2_HUMAN RRGRQLPQLPPKG (1157-1169) NCK1_HUMAN DOCK3_HUMAN GDTPPALPARTLRK (1848-1861) NCK1_HUMAN ECT2_HUMAN NIVKDLPFEPSKK (285-297) NCK1_HUMAN ABL1_HUMAN KKKKTAPTPPKRS (606-618) NCK1_HUMAN EP15_HUMAN TPTRPCPLPPGKR (777-789) NCK1_HUMAN HNRPR_HUMAN RGGRGAPPPPRGR (506-518) NCK1_HUMAN GABR1_HUMAN FFSDPAVPVKNLKR (343-356) NCK1_HUMAN LAP2B_HUMAN DIPRRAPKKPLTR (315-327) NCK1_HUMAN SHAN2_HUMAN MVDKPPVPPKPKMK (944-957) NCK1_HUMAN P3C2B_HUMAN LNQLPPCPSRMQPK (555-568) NCK1_HUMAN SYNJ2_HUMAN PPLVPKVPPRRKKS (1326-1339) NCK1_HUMAN CNTP1_HUMAN FVQVPGFPRRGRLA (371-384) NCK1_HUMAN ABL2_HUMAN MKKRNAPTPPKRS (657-669) NCK1_HUMAN DDEF1_HUMAN PRVLPKLPQKVALR (718-731) NCK1_HUMAN PAR3_HUMAN NLAKPTLPIKTFRG (29-42) NCK1_HUMAN ELK3_HUMAN KTKSPSLPPKAKKP (281-294) NCK1_HUMAN CELR3_HUMAN FRCRFLPQRPGPR (282-294) NCK1_HUMAN ID4_HUMAN RLRRLVPTIPPNK (74-86) NCK1_HUMAN FLNB_HUMAN AKLKPGAPLKPKLN (240-253) NCK1_HUMAN RIMS1_HUMAN RRGRQLPQVPVRS (1266-1278) NCK1_HUMAN PTN4_HUMAN DGKPPALPPKQSKK (459-472) NCK1_HUMAN ARHGB_HUMAN DKDKWLPFFPKTK (504-516) NCK1_HUMAN BRPF3_HUMAN NSEQPQFPGKSKKP (89-102) NCK1_HUMAN RAGE_HUMAN LKCKGAPKKPPQR (36-48) NCK1_HUMAN MYLK_HUMAN VKKKPAPKTPPKA (1222-1234) NCK1_HUMAN TRAIP_HUMAN VMIRPLPVKPKTK (433-445) NCK1_HUMAN DCE1_HUMAN QSLRGVPDSPQRR (520-532) NCK1_HUMAN RIN3_HUMAN PAKKNLPTAPPRR (377-389) NCK1_HUMAN NEK8_HUMAN VRCRGIPRGPVRP (293-305) NCK1_HUMAN FOXH1_HUMAN RCFRKVPKDPAKP (89-101) NCK1_HUMAN MINT_HUMAN QSKKPIPSKPQLK (1102-1114) NCK1_HUMAN CSMD2_HUMAN MCPDPGIPERGKRL (342-355) NCK1_HUMAN MLL4_HUMAN EPGGPPAPRRRTPR (1096-1109) NCK1_HUMAN RRAS_HUMAN PPSPPSAPRKKGGG (199-212) NCK1_HUMAN M4K5_HUMAN SAQAPQLPRKNDKR (463-476) NCK1_HUMAN BAZ2A_HUMAN FTQKPGFPKRGQKR (1705-1718) NCK1_HUMAN ABL1_HUMAN KKKKTAPTPPKRS (606-618) NCK1_HUMAN HNRPR_HUMAN RGGRGAPPPPRGR (506-518) NCK1_HUMAN EP15_HUMAN TPTRPCPLPPGKR (777-789) NCK1_HUMAN DLP1_HUMAN RRAPPPVPKKPAKG (910-923) NCK1_HUMAN SREC2_HUMAN SMKLPRIPLRRQKL (496-509) NCK1_HUMAN GABR1_HUMAN FFSDPAVPVKNLKR (343-356) NCK1_HUMAN ABL2_HUMAN MKKRNAPTPPKRS (657-669) NCK1_HUMAN SYNJ2_HUMAN PPLVPKVPPRRKKS (1326-1339) NCK1_HUMAN SNX17_HUMAN ANVLPAFPPKKLFS (53-66) NCK1_HUMAN M4K1_HUMAN EDTPPPLPPKPKFR (391-404) NCK1_HUMAN DOCK3_HUMAN GDTPPALPARTLRK (1848-1861) NCK1_HUMAN SHAN3_HUMAN LLEKPPVPPKPKLK (472-485) NCK1_HUMAN DLP2_HUMAN KKTPPPVPPRTTSK (575-588) NCK1_HUMAN DLP4_HUMAN KKTPPPVPPRTTSK (558-571) NCK1_HUMAN MINT_HUMAN QSKKPIPSKPQLK (1102-1114) NCK1_HUMAN SOS2_HUMAN CKQPPRFPRKSTFS (1019-1032) NCK1_HUMAN ELK3_HUMAN KTKSPSLPPKAKKP (281-294) NCK1_HUMAN PTN4_HUMAN DGKPPALPPKQSKK (459-472) NCK1_HUMAN GPR45_HUMAN FQILPKVPERIRRR (343-356) NCK1_HUMAN FCG2B_HUMAN VAGTPAAPPKAVLK (40-53) NCK1_HUMAN FCG2C_HUMAN VAGTPAAPPKAVLK (40-53) NCK1_HUMAN EFS_HUMAN NRVKLLPAGPAPK (61-73) NCK1_HUMAN RAGE_HUMAN LKCKGAPKKPPQR (36-48) NCK1_HUMAN CELR3_HUMAN FRCRFLPQRPGPR (282-294) NCK1_HUMAN M4K3_HUMAN AKIPPPLPPKPKSI (429-442) NCK1_HUMAN SHAN2_HUMAN MVDKPPVPPKPKMK (944-957) NCK1_HUMAN RIN3_HUMAN PAKKNLPTAPPRR (377-389) NCK1_HUMAN DUS15_HUMAN VADTPEVPIKKHFK (52-65) NCK1_HUMAN TRAIP_HUMAN VMIRPLPVKPKTK (433-445) NCK1_HUMAN DDEF1_HUMAN PRVLPKLPQKVALR (718-731) NCK1_HUMAN FLNB_HUMAN AKLKPGAPLKPKLN (240-253) NCK1_HUMAN LAP2B_HUMAN DIPRRAPKKPLTR (315-327) NCK1_HUMAN ECT2_HUMAN NIVKDLPFEPSKK (285-297) NCK1_HUMAN DAB2_HUMAN QQAAPKAPSKKEKK (16-29) NCK1_HUMAN NAP5_HUMAN PNFRTAPGMPSRR (210-222) NCK1_HUMAN SOS1_HUMAN RNPKPLPRFPKKY (1019-1031) NCK1_HUMAN RRAS_HUMAN PPSPPSAPRKKGGG (199-212) NCK1_HUMAN BRPF3_HUMAN NSEQPQFPGKSKKP (89-102) NCK1_HUMAN DLP3_HUMAN KKVPPPIPKKPLRG (256-269) NCK1_HUMAN ARHGB_HUMAN DKDKWLPFFPKTK (504-516) NCK1_HUMAN DAG1_HUMAN ANKKPPLPKRVRRQ (300-313) NCK1_HUMAN MAP4_HUMAN PPNKELPPSPEKK (688-700) NCK1_HUMAN NPA1P_HUMAN DKNKPVMPARAKGP (1993-2006) NCK1_HUMAN M4K5_HUMAN SAQAPQLPRKNDKR (463-476) NCK1_HUMAN H14_HUMAN AKPKKAPKSPAKA (178-190) NCK1_HUMAN IL3RA_HUMAN VMQRLFPRIPHMK (330-342) NCK1_HUMAN P3C2B_HUMAN LNQLPPCPSRMQPK (555-568) NCK1_HUMAN FLNC_HUMAN AKLKPGAPVRSKQL (260-273) NCK1_HUMAN CNTP1_HUMAN FVQVPGFPRRGRLA (371-384) NCK1_HUMAN TPX2_HUMAN ISQEPFVPKKEKKS (633-646) NCK1_HUMAN DCE1_HUMAN QSLRGVPDSPQRR (520-532) NCK1_HUMAN GHR_HUMAN DAKKCIPVAPHIK (539-551) NCK1_HUMAN AKAP6_HUMAN SPTQPTLPKRGLFL (383-396) NCK1_HUMAN CCR10_HUMAN QPRRGCPRRPRLS (335-347) NCK1_HUMAN LMO6_HUMAN LLGRPFLPRRGLIF (348-361) NCK1_HUMAN RIMS2_HUMAN RRGRQLPQLPPKG (1157-1169) NCK1_HUMAN GASR_HUMAN TCARCCPRPPRAR (404-416) NCK1_HUMAN MYLK_HUMAN VKKKPAPKTPPKA (1222-1234) NCK1_HUMAN WASL_HUMAN ARGRGAPPPPPSR (304-316) NCK1_HUMAN TLR9_HUMAN QAAVPGLPSRVKCG (778-791) NCK1_HUMAN TNIK_HUMAN RQSSPAMPHKVANR (545-558) NCK1_HUMAN MLL4_HUMAN EPGGPPAPRRRTPR (1096-1109) NCK1_HUMAN BAT8_HUMAN HATKSFPSSPSKG (111-123) NCK1_HUMAN CNTFR_HUMAN VVARPVPSNPRRL (210-222) NCK1_HUMAN ID4_HUMAN RLRRLVPTIPPNK (74-86) NCK1_HUMAN SF3B4_HUMAN PTPRPPVPPRGPLR (406-419) NCK1_HUMAN NXPH3_HUMAN GQPRPRVPRKRGHI (40-53) NCK1_HUMAN NEU1_HUMAN DVRKCLPCGPGGK (37-49) NCK1_HUMAN CAF1A_HUMAN CKDRPAFPVKKLIQ (3-16) NCK1_HUMAN STF1_HUMAN GYLYPAFPGRAIKS (182-195) NCK1_HUMAN RL13_HUMAN QLTGPVMPVRNVYK (148-161) NCK1_HUMAN CRSP2_HUMAN MGDDPDVPWRLLKL (237-250) NCK1_HUMAN RPGF1_HUMAN TDTPPALPEKKRRS (450-463) NCK1_HUMAN MYRIP_HUMAN QQRRKLPAPPVKA (798-810) NCK1_HUMAN ABCG5_HUMAN KHLKTLPMVPFKT (346-358) NCK1_HUMAN SUV92_HUMAN CQCGPDCPNRIVQK (233-246) NCK1_HUMAN PAR3_HUMAN NLAKPTLPIKTFRG (29-42) NCK1_HUMAN P2RX7_HUMAN QEQRLCPEYPTRR (114-126) NCK1_HUMAN RYR1_HUMAN PPKKQAPPSPPPK (4521-4533) NCK1_HUMAN RAG1_HUMAN DGQKPVPTQPLLK (70-82) NCK1_HUMAN APOL5_HUMAN HQPAPPAPARKGRQ (413-426) NCK1_HUMAN BRD4_HUMAN EPKTPVAPKKDLKI (1183-1196) NCK1_HUMAN RFRP_HUMAN SLVRRVPNLPQRF (119-131) NCK1_HUMAN JAK2_HUMAN QFTKCCPPKPKDK (494-506) NCK1_HUMAN DOCK1_HUMAN FALEPLLPKKMHSR (1667-1680) NCK1_HUMAN FYB_HUMAN QPPVPSLPPRNIKP (413-426) P85A_HUMAN SOS2_HUMAN PLIPPPLPPRKKFD (1143-1156) P85A_HUMAN PTN4_HUMAN DGKPPALPPKQSKK (459-472) P85A_HUMAN DOCK3_HUMAN GDTPPALPARTLRK (1848-1861) P85A_HUMAN SNX17_HUMAN ANVLPAFPPKKLFS (53-66) P85A_HUMAN DLP2_HUMAN KKTPPPVPPRTTSK (575-588) P85A_HUMAN DLP4_HUMAN KKTPPPVPPRTTSK (558-571) P85A_HUMAN DAB2_HUMAN QQAAPKAPSKKEKK (16-29) P85A_HUMAN SHAN3_HUMAN LLEKPPVPPKPKLK (472-485) P85A_HUMAN DUS15_HUMAN VADTPEVPIKKHFK (52-65) P85A_HUMAN FLNA_HUMAN AKLKPGAPLRPKLN (267-280) P85A_HUMAN SYNJ2_HUMAN PPLVPKVPPRRKKS (1326-1339) P85A_HUMAN ID4_HUMAN RLRRLVPTIPPNK (74-86) P85A_HUMAN ABL1_HUMAN KKKKTAPTPPKRS (606-618) P85A_HUMAN SUV92_HUMAN CQCGPDCPNRIVQK (233-246) P85A_HUMAN FLNB_HUMAN AKLKPGAPLKPKLN (240-253) P85A_HUMAN ABL2_HUMAN MKKRNAPTPPKRS (657-669) P85A_HUMAN SHAN2_HUMAN MVDKPPVPPKPKMK (944-957) P85A_HUMAN DLP1_HUMAN KKTPPPVPPRTTTK (539-552) P85A_HUMAN SOS1_HUMAN RNPKPLPRFPKKY (1019-1031) P85A_HUMAN DDEF2_HUMAN TTSAPPLPPRNVGK (781-794) P85A_HUMAN LIPA3_HUMAN ALGSPGLPLRKLQP (1161-1174) P85A_HUMAN AKAP2_HUMAN TKNAPSLPSRTCYK (982-995) P85A_HUMAN ARHGB_HUMAN DKDKWLPFFPKTK (504-516) P85A_HUMAN CSF2R_HUMAN RIQRLFPPVPQIK (351-363) P85A_HUMAN ELK3_HUMAN KTKSPSLPPKAKKP (281-294) P85A_HUMAN RL13_HUMAN QLTGPVMPVRNVYK (148-161)

P85A_HUMAN BRCA1_HUMAN YRIPPLFPIKSFVK (978-991) P85A_HUMAN TPX2_HUMAN ISQEPFVPKKEKKS (633-646) P85A_HUMAN RRAS_HUMAN PPSPPSAPRKKGGG (199-212) P85A_HUMAN ECT2_HUMAN NIVKDLPFEPSKK (285-297) P85A_HUMAN JAK2_HUMAN QFTKCCPPKPKDK (494-506) P85A_HUMAN DDEF1_HUMAN LGPRVLPKLPQKV (716-728) P85A_HUMAN FLNC_HUMAN AKLKPGAPVRSKQL (260-273) P85A_HUMAN DNL3_HUMAN RSLKPVLPHKVAHF (441-454) P85A_HUMAN ABCG5_HUMAN LKTLPMVPFKTKDS (348-361) P85A_HUMAN BRPF3_HUMAN NSEQPQFPGKSKKP (89-102) P85A_HUMAN SH3K1_HUMAN KPSVPAIPPKKPRP (392-405) P85A_HUMAN MYRIP_HUMAN QQRRKLPAPPVKA (798-810) P85A_HUMAN HC127_HUMAN QIHRNAPICPLCK (195-207) P85A_HUMAN PIPPI_HUMAN VTYKMCPIPPKNQ (113-125) P85A_HUMAN DAG1_HUMAN ANKKPPLPKRVRRQ (300-313) P85A_HUMAN STF1_HUMAN GYLYPAFPGRAIKS (182-195) P85A_HUMAN P3C2B_HUMAN LNQLPPCPSRMQPK (555-568) P85A_HUMAN BRD4_HUMAN NAPPPGAPDKDKQK (1168-1181) P85A_HUMAN DLP3_HUMAN KKVPPPIPKKPLRG (256-269) P85A_HUMAN MINT_HUMAN QSKKPIPSKPQLK (1102-1114) P85A_HUMAN GBRA3_HUMAN KKKTPAAPAKKTST (381-394) P85A_HUMAN PLCG2_HUMAN SEINPSMPQRTVKA (763-776) P85A_HUMAN MAP4_HUMAN PPNKELPPSPEKK (688-700) P85A_HUMAN GABR1_HUMAN FFSDPAVPVKNLKR (343-356) P85A_HUMAN MYLK_HUMAN VKKKPAPKTPPKA (1222-1234) P85A_HUMAN PEBP_HUMAN VLTDPDAPSRKDPK (66-79) P85A_HUMAN EFS_HUMAN NRVKLLPAGPAPK (61-73) P85A_HUMAN RPGF1_HUMAN NSPPPALPPKKRQS (280-293) P85A_HUMAN M4K1_HUMAN EDTPPPLPPKPKFR (391-404) P85A_HUMAN CAF1A_HUMAN CKDRPAFPVKKLIQ (3-16) P85A_HUMAN DTBP1_HUMAN SKPRTVPFLPKYS (34-46) P85A_HUMAN AKAP6_HUMAN SPTQPTLPKRGLFL (383-396) P85A_HUMAN FOXH1_HUMAN RCFRKVPKDPAKP (89-101) P85A_HUMAN GHR_HUMAN DAKKCIPVAPHIK (539-551) P85A_HUMAN APOL5_HUMAN HQPAPPAPARKGRQ (413-426) PLCG1_HUMAN DUS15_HUMAN VADTPEVPIKKHFK (52-65) PLCG1_HUMAN TRAIP_HUMAN VMIRPLPVKPKTK (433-445) PLCG1_HUMAN SNX17_HUMAN ANVLPAFPPKKLFS (53-66) PLCG1_HUMAN DDEF2_HUMAN TTSAPPLPPRNVGK (781-794) PLCG1_HUMAN ARHGB_HUMAN DKDKWLPFFPKTK (504-516) PLCG1_HUMAN RL13_HUMAN QLTGPVMPVRNVYK (148-161) PLCG1_HUMAN DDEF1_HUMAN TTEAPPLPPRNAGK (614-627) PLCG1_HUMAN RRAS_HUMAN PPSPPSAPRKKGGG (199-212) PLCG1_HUMAN M4K1_HUMAN EDTPPPLPPKPKFR (391-404) PLCG1_HUMAN ABL2_HUMAN SSGSPALPRKQRDK (617-630) PLCG1_HUMAN EFS_HUMAN NRVKLLPAGPAPK (61-73) PLCG1_HUMAN GHR_HUMAN DAKKCIPVAPHIK (539-551) PLCG1_HUMAN RYR1_HUMAN PPKKQAPPSPPPK (4521-4533) PLCG1_HUMAN RGS7_HUMAN NVGRNIPIFPCHK (470-482) PLCG1_HUMAN SHAN2_HUMAN MVDKPPVPPKPKMK (944-957) PLCG1_HUMAN SOS2_HUMAN PLIPPPLPPRKKFD (1143-1156) PLCG1_HUMAN JAK2_HUMAN QFTKCCPPKPKDK (494-506) PLCG1_HUMAN SOS1_HUMAN PKPLPRFPKKYSYP (1021-1034) PLCG1_HUMAN FLNB_HUMAN AKLKPGAPLKPKLN (240-253) PLCG1_HUMAN ID4_HUMAN RLRRLVPTIPPNK (74-86) PLCG1_HUMAN FLNA_HUMAN AKLKPGAPLRPKLN (267-280) PLCG1_HUMAN IL3RA_HUMAN VMQRLFPRIPHMK (330-342) PLCG1_HUMAN ABL1_HUMAN KKKKTAPTPPKRS (606-618) PLCG1_HUMAN SUV92_HUMAN CQCGPDCPNRIVQK (233-246) PLCG1_HUMAN ZP2_HUMAN TLTIPEFPGKLKSV (274-287) PLCG1_HUMAN FCG2B_HUMAN VAGTPAAPPKAVLK (40-53) PLCG1_HUMAN FCG2C_HUMAN VAGTPAAPPKAVLK (40-53) PLCG1_HUMAN CSMD2_HUMAN MCPDPGIPERGKRL (342-355) PLCG1_HUMAN ANT3_HUMAN AKPRDIPMNPMCI (42-54) PLCG1_HUMAN SHAN3_HUMAN LLEKPPVPPKPKLK (472-485) PLCG1_HUMAN CAF1A_HUMAN PQIKSLPATPQGK (233-245) PLCG1_HUMAN MAP4_HUMAN PPNKELPPSPEKK (688-700) PLCG1_HUMAN TDRD1_HUMAN SLMRLCPIIPKLL (183-195) PLCG1_HUMAN DNL3_HUMAN RSLKPVLPHKVAHF (441-454) PLCG1_HUMAN RIN3_HUMAN QVPAPPLPAKKNLP (370-383) PLCG1_HUMAN RAG1_HUMAN DGQKPVPTQPLLK (70-82) PLCG1_HUMAN DTBP1_HUMAN SKPRTVPFLPKYS (34-46) PLCG1_HUMAN AKAP2_HUMAN TKNAPSLPSRTCYK (982-995) PLCG1_HUMAN FLNC_HUMAN AKLKPGAPVRSKQL (260-273) PLCG1_HUMAN DLP1_HUMAN KKTPPPVPPRTTTK (539-552) PLCG1_HUMAN MINT_HUMAN QSKKPIPSKPQLK (1102-1114) PLCG1_HUMAN LIPA4_HUMAN TLQPPPAPPKKIMP (671-684) PLCG1_HUMAN PAR3_HUMAN NLAKPTLPIKTFRG (29-42) PLCG1_HUMAN DLP3_HUMAN KKVPPPIPKKPLRG (256-269)

*Proteins known or predicted to function in signal transduction and/or cell-cell communication are listed. Both GO annotations and key words in protein description (ie., G-protein, GTPase, receptor, adaptor, kinase, phosphatase, adhesion, signal transduction, signal pathway, binding, interacting, associated, domain) are used for the classification (see also reference 28). For a complete list of binary protein-protein interactions identified by PATS, please visit http://129.100.30.140/PATS.htm

Table S3: Comparison of SH3 interactions discovered by PATS with OPHID

SH3 protein interacting protein Source Notes from reference* Ref. CRK_HUMAN ABL2_HUMAN HPRD SH3 binding [1] CRK_HUMAN DOCK1_HUMAN HPRD SH3 binding [2] CRK_HUMAN M4K1_HUMAN HPRD SH3 binding [3] CRK_HUMAN RPGF1_HUMAN MINT SH3 binding [4] CRK_HUMAN SOS1_HUMAN HPRD Yeast 2-hybrid [5] FYN_HUMAN BCA1_HUMAN HPRD SH3 binding [6] FYN_HUMAN CD2AP_HUMAN HPRD,MINT SH3 binding [7] FYN_HUMAN FYB_HUMAN HPRD,MINT Predicted [8] FYN_HUMAN SOS1_HUMAN HPRD SH3 binding [9] GRB2_HUMAN ABL1_HUMAN HPRD SH3 binding [10] GRB2_HUMAN DAB2_HUMAN HPRD SH3 binding [11] GRB2_HUMAN DAG1_HUMAN HPRD SH3 binding [12] GRB2_HUMAN DYN1_HUMAN HPRD SH3 binding [13] GRB2_HUMAN GHR_HUMAN HPRD GHR bind to GRB10 via SH2 domain [14] GRB2_HUMAN JAK2_HUMAN HPRD SH2 binding. [15] GRB2_HUMAN M4K1_HUMAN HPRD,BIND SH3 binding [16] GRB2_HUMAN P3C2B_HUMAN HPRD SH3 binding [17] GRB2_HUMAN RPGF1_HUMAN MINT Predicted from yeast [18] GRB2_HUMAN SF3B4_HUMAN FlyLow Predicted from fly BIND,RikenBI SH3-binding [19] GRB2_HUMAN SOS1_HUMAN ND,HPRD GRB2_HUMAN SOS2_HUMAN HPRD SH3-binding [20] GRB2_HUMAN WASL_HUMAN HPRD Prediction, WASP bind GRB2 through SH3-peptide interaction [21] NCK1_HUMAN ABL1_HUMAN HPRD SH3 binding [22] NCK1_HUMAN RRAS_HUMAN HPRD SH3 binding [23] NCK1_HUMAN SOS1_HUMAN HPRD Predicted [24] NCK1_HUMAN WASL_HUMAN HPRD Predicted; SH3-binding [25] P85A_HUMAN ABL1_HUMAN BIND Predicted [26] P85A_HUMAN CSF2R_HUMAN HPRD SH3-binding [27] P85A_HUMAN GHR_HUMAN HPRD SH2 binding [14] P85A_HUMAN JAK2_HUMAN HPRD SH2 binding [27] P85A_HUMAN SH3K1_HUMAN HPRD Predicted from SH3K1_RAT [28] PLCG1_HUMAN ABL1_HUMAN BIND Predicted [26] PLCG1_HUMAN SOS1_HUMAN HPRD SH3 binding [29] PLCG1_HUMAN SOS2_HUMAN HPRD SH3 binding [20] SRC_HUMAN ABL1_HUMAN BIND Predicted [26]

*Of the 35 protein-protein interaction matches between OPHID and PATS, 22 have been verified by different methods to be directly mediated by SH3-peptide binding and the remaining 13 (marked yellow) are either predicted interactions based on orthologs from other species or interactions where SH2 domains have been shown to play a key role, suggesting domain cooperation in mediating a binding event.

References to Table S3

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A role for p59(Fyn) and SHP2. J Biol Chem, 1997. 272(25): p. 15636-41. 7. Kirsch, K.H., et al., CMS: an adapter molecule involved in cytoskeletal rearrangements. Proc Natl Acad Sci U S A, 1999. 96(11): p. 6211-6. 8. da Silva, A.J., O. Janssen, and C.E. Rudd, T cell receptor zeta/CD3-p59fyn(T)- associated p120/130 binds to the SH2 domain of p59fyn(T). J Exp Med, 1993. 178(6): p. 2107-13. 9. Park, C., Y. Choi, and Y. Yun, Son of sevenless binds to the SH3 domain of src- type tyrosine kinase. Mol Cells, 1998. 8(5): p. 518-23. 10. Romero, F., et al., Grb2 and its apoptotic isoform Grb3-3 associate with heterogeneous nuclear ribonucleoprotein C, and these interactions are modulated by poly(U) RNA. J Biol Chem, 1998. 273(13): p. 7776-81. 11. Xu, X.X., et al., Disabled-2 (Dab2) is an SH3 domain-binding partner of Grb2. Oncogene, 1998. 16(12): p. 1561-9. 12. Yang, B., et al., SH3 domain-mediated interaction of dystroglycan and Grb2. J Biol Chem, 1995. 270(20): p. 11711-4. 13. Miki, H., et al., Association of Ash/Grb-2 with dynamin through the Src homology 3 domain. J Biol Chem, 1994. 269(8): p. 5489-92. 14. Moutoussamy, S., et al., Grb10 identified as a potential regulator of growth hormone (GH) signaling by cloning of GH receptor target proteins. J Biol Chem, 1998. 273(26): p. 15906-12. 15. Chauhan, D., et al., Oncostatin M induces association of Grb2 with Janus kinase JAK2 in multiple myeloma cells. J Exp Med, 1995. 182(6): p. 1801-6. 16. Ling, P., et al., Interaction of hematopoietic progenitor kinase 1 with adapter proteins Crk and CrkL leads to synergistic activation of c-Jun N-terminal kinase. Mol Cell Biol, 1999. 19(2): p. 1359-68. 17. Wheeler, M. and J. Domin, Recruitment of the class II phosphoinositide 3-kinase C2beta to the epidermal growth factor receptor: role of Grb2. Mol Cell Biol, 2001. 21(19): p. 6660-7. 18. Smit, L., G. van der Horst, and J. Borst, Sos, Vav, and C3G participate in B cell receptor-induced signaling pathways and differentially associate with Shc-Grb2, Crk, and Crk-L adaptors. J Biol Chem, 1996. 271(15): p. 8564-9. 19. Puil, L., et al., Bcr-Abl oncoproteins bind directly to activators of the Ras signalling pathway. Embo J, 1994. 13(4): p. 764-73. 20. Scholler, J.K., et al., Engagement of the T lymphocyte antigen receptor regulates association of son-of-sevenless homologues with the SH3 domain of phospholipase Cgamma1. Eur J Immunol, 2000. 30(8): p. 2378-87. 21. Carlier, M.F., et al., GRB2 links signaling to actin assembly by enhancing interaction of neural Wiskott-Aldrich syndrome protein (N-WASp) with actin- related protein (ARP2/3) complex. J Biol Chem, 2000. 275(29): p. 21946-52. 22. Smith, J.M., S. Katz, and B.J. Mayer, Activation of the Abl tyrosine kinase in vivo by Src homology 3 domains from the Src homology 2/Src homology 3 adaptor Nck. J Biol Chem, 1999. 274(39): p. 27956-62. 23. Wang, B., et al., R-Ras contains a proline-rich site that binds to SH3 domains and is required for integrin activation by R-Ras. J Biol Chem, 2000. 275(7): p. 5222- 7. 24. De Sepulveda, P., et al., Socs1 binds to multiple signalling proteins and suppresses steel factor-dependent proliferation. Embo J, 1999. 18(4): p. 904-15. 25. Rohatgi, R., et al., Nck and phosphatidylinositol 4,5-bisphosphate synergistically activate actin polymerization through the N-WASP-Arp2/3 pathway. J Biol Chem, 2001. 276(28): p. 26448-52. 26. Muller, A.J., et al., A limited set of SH2 domains binds BCR through a high- affinity phosphotyrosine-independent interaction. Mol Cell Biol, 1992. 12(11): p. 5087-93. 27. Dhar-Mascareno, M., et al., Granulocyte-macrophage colony-stimulating factor signals for increased glucose transport via phosphatidylinositol 3-kinase- and hydrogen peroxide-dependent mechanisms. J Biol Chem, 2003. 278(13): p. 11107-14. 28. Gout, I., et al., Negative regulation of PI 3-kinase by Ruk, a novel adaptor protein. Embo J, 2000. 19(15): p. 4015-25. 29. Kim, M.J., et al., Direct interaction of SOS1 Ras exchange protein with the SH3 domain of phospholipase C-gamma1. Biochemistry, 2000. 39(29): p. 8674-82.

PLCγ1 SH3 Grb2 SH3(N) SH3 or f-SH3 SH3 SH3 f-SH3 SH3 SH3 f-SH3 Bt-Peptide - + + - + + Beads + + + + + + 15 10 MW (kDa)

Figure S1 Wu et al. Figure S2