Supplementary File for ALLEN, MARGARET LOUISE− Greenhouse evaluation of neonate and adult applications of Coleomegilla maculata (Coleoptera: Coccinellidae) to control twospotted spider mite infestations. Florida Entomologist 98(2) (June, 2015) at http://purl.fcla.edu/fcla/entomologist/browse .
Margaret L. Allen National Biological Control Laboratory, USDA Agricultural Research Service 59 Lee Road, Stoneville, Mississippi 38776, USA Phone: 662-686-3647; Fax: 662-686-5281 Email: [email protected]
Abstract The lady beetle Coleomegilla maculata DeGeer (Coleoptera: Coccinellidae) is a euryphagous predator that is known to consume the twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Spider mites are frequently problematic in greenhouse operations, and often cause damage to the ornamental and bedding plant Impatiens walleriana Hook f. (Ericales: Balsaminaceae). Increasing demand for alternatives to pesticides combined with resistance to registered pest control products necessitates the evaluation of viable alternatives. In this study, naïve impatiens were intentionally infested with twospotted spider mites. When damage was visible on experimental plants, they were treated with lady beetles in containment cages. One treatment was adult C. maculata and another treatment was neonate C. maculata using recently developed methods for rearing and harvesting eggs. Levels of spider mite infestation were assessed after treatments, and images of the treated and untreated plants were collected to assess the effectiveness of C. maculata in a greenhouse environment. Plants with C. maculata treatments were essentially free of spider mites. There is potential to use mass reared C. maculata eggs as treatment for spider mites in contained crops or indoor decorative plants.
Key Words: lady beetle, Tetranychus urticae, impatiens, ornamental plant, generalist predator
Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents
Slides 3 through 122 show the status of plants from day 1 to day 40 after treatment. Slides 3-32 : Negative control, no mite infestation, no treatment
Slides 33-62: Positive control, mite infestation, no treatment
Slides 63-92: Treatment: mite infestation, adult beetles added
Slides 93-122: Treatment: mite infestation, neonate beetles added
Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 2: Control – uninfested, no beetles
Tent 2 will be shown in upper left. Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 7: Control – uninfested, no beetles
Tent 7 will be shown in upper right. Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 12: Control – uninfested, no beetles
Tent 12 will be shown in lower left. Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 13: Control – uninfested, no beetles
Tent 13 will be shown in lower right. Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Negative Control – uninfested, no beetles
Tent 2 Tent 7
Tent 12 Tent 13 Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 4: Spider mite infested, no beetle treatment.
Tent 4 will be shown in upper left. Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 5: Spider mite infested, no beetle treatment
Tent 5 will be shown in upper right. Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 10: Spider mite infested, no beetle treatment.
Tent 10 will be shown in lower left. Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 15: Spider mite infested, no beetle treatment
Tent 15 will be shown in lower right. Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
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Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
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Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
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Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Positive Control – Mite Infested, No Treatment
Tent 4 Tent 5
Tent 10 Tent 15 Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 3: Spider mite infestation, adult beetle treatment.
Tent 3 will be shown in upper left. Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 8: Spider mite infestation, adult beetle treatment.
Tent 8 will be shown in upper right. Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 9: Spider mite infestation, adult beetle treatment.
Tent 9 will be shown in lower left.
Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 14: Spider mite infestation, adult beetle treatment.
Tent 14 will be shown in lower right. Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Treatment – Mite Infested + Adult Beetles
Tent 3 Tent 8
Tent 9 Tent 14 Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 1: Spider mite infestation, neonate beetle treatment.
Tent 1 will be shown in upper left. Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 6: Spider mite infestion, neonate beetle treatment
Tent 6 will be shown in upper right. Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 11: Spider mite infestation, neonate beetle treatment.
Tent 11 will be shown in lower left. Impatiens, spider mites, and lady beetles (Coleomegilla maculata) in experimental tents.
Tent 16: Spider mite infested with neonate beetles treatment
Tent 16 will be shown bottom right. Treatment – Mite Infested + Neonate Beeltes
Tent 1 Tent 6
Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
Tent 1 Tent 6
Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
Tent 1 Tent 6
Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
Tent 1 Tent 6
Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
Tent 1 Tent 6
Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
Tent 1 Tent 6
Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
Tent 1 Tent 6
Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
Tent 1 Tent 6
Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
Tent 1 Tent 6
Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16 Treatment – Mite Infested + Neonate Beeltes
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Tent 11 Tent 16