SANTA CRUZ BIOTECHNOLOGY, INC. USP6/32 (D-11): sc-377306

BACKGROUND APPLICATIONS -specific protease (USP6) is a 1,406 amino acid protein that is very USP6/32 (D-11) is recommended for detection of USP6 isoforms 1 and 2, hydrophilic and has 2 charge clusters that are characteristic of nucleic acid- and USP32 of human origin, and USP32 of mouse and rat origin by Western binding regions. The USP6 is oncogenic and originated from the chimeric Blotting (starting dilution 1:100, dilution range 1:100-1:1000), immunopre- fusion of two : USP32 and TBC1D3. USP32 is an ancient, highly con- cipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immu- served gene, whereas the TBC1D3 gene came from a relatively recent seg- nofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid mental duplication. This duplication is absent in most other mammals and phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). shows rapid amplification and propagation throughout the primate lineage. Suitable for use as control antibody for USP32 siRNA (m): sc-106679, USP32 The chimeric USP6, however, only exists in the hominoid lineage of primates, shRNA Plasmid (m): sc-106679-SH and USP32 shRNA (m) Lentiviral Particles: so it may have contributed to hominoid speciation. USP6 is testis-specific, sc-106679-V. suggesting an implication in the emergence of reproductive barriers. Molecular Weight of USP6: 159 kDa. REFERENCES Molecular Weight of USP32: 182 kDa. 1. White, R.A., et al. 2000. The gene encoding TBC1D1 with homology to the Positive Controls: human testis extract: sc-363781 or Caki-1 cell lysate: Tre2/USP6 oncogene, BUB2, and Cdc16 maps to mouse 5 and sc-2224. human chromosome 4. Cytogenet. Cell Genet. 89: 272-275. 2. Online Mendelian Inheritance in Man, OMIM™. 2002. Johns Hopkins RECOMMENDED SUPPORT REAGENTS University, Baltimore, MD. MIM Number: 604334. World Wide Web URL: To ensure optimal results, the following support reagents are recommended: http://www.ncbi.nlm.nih.gov/omim/ 1) Western Blotting: use m-IgGκ BP-HRP: sc-516102 or m-IgGκ BP-HRP (Cruz 3. Paulding, C.A., et al. 2003. The Tre2/USP6 oncogene is a hominoid-specific Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ gene. Proc. Natl. Acad. Sci. USA 100: 2507-2511. Molecular Weight Standards: sc-2035, UltraCruz® Blocking Reagent: sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopre- 4. Oliveira, A.M., et al. 2004. USP6/Tre2 fusion oncogenes in aneurysmal bone cipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). cyst. Cancer Res. 64: 1920-1923. 3) Immunofluorescence: use m-IgGκ BP-FITC: sc-516140 or m-IgGκ BP-PE: 5. Oliveira, A.M., et al. 2004. USP6 and CDH11 oncogenes identify the neo- sc-516141 (dilution range: 1:50-1:200) with UltraCruz® Mounting Medium: plastic cell in primary aneurysm bone cysts and are absent in so-called sc-24941 or UltraCruz® Hard-set Mounting Medium: sc-359850. secondary aneurysmal bone cysts. Am. J. Pathol. 165: 1773-1780. DATA CHROMOSOMAL LOCATION

Genetic locus: USP6 (human) mapping to 17p13.2, USP32 (human) mapping AB to 17q23.1; Usp32 (mouse) mapping to 11 C. 222K–

SOURCE USP6/32 USP6/32 (D-11) is a mouse monoclonal antibody raised against amino acids 107K– 1117-1406 mapping at the C-terminus of USP6 of human origin.

PRODUCT USP6/32 (D-11): sc-377306.Western blot analysisof USP6/32 (D-11): sc-377306. Immunofluorescence USP6/32 expression in Caki-1 wholecell lysate (A) staining of methanol-fixed HeLa cells showing Golgi Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% and human testistissueextract (B). apparatus and cytoplasmic localization. sodium azide and 0.1% gelatin. USP6/32 (D-11) is available conjugated to agarose (sc-377306 AC), 500 µg/ SELECT PRODUCT CITATIONS 0.25 ml agarose in 1 ml, for IP; to HRP (sc-377306 HRP), 200 µg/ml, for 1. Long, C., et al. 2018. LPS promotes HBO1 stability via USP25 to modulate WB, IHC(P) and ELISA; to either phycoerythrin (sc-377306 PE), fluorescein inflammatory gene transcription in THP-1 cells. Biochim. Biophys. Acta (sc-377306 FITC), Alexa Fluor® 488 (sc-377306 AF488), Alexa Fluor® 546 Gene Regul. Mech. 1861: 773-782. (sc-377306 AF546), Alexa Fluor® 594 (sc-377306 AF594) or Alexa Fluor® 647 (sc-377306 AF647), 200 µg/ml, for WB (RGB), IF, IHC(P) and FCM; STORAGE and to either Alexa Fluor® 680 (sc-377306 AF680) or Alexa Fluor® 790 Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of (sc-377306 AF790), 200 µg/ml, for Near-Infrared (NIR) WB, IF and FCM. shipment. Non-hazardous. No MSDS required. RESEARCH USE Alexa Fluor® is a trademark of Molecular Probes, Inc., Oregon, USA For research use only, not for use in diagnostic procedures.

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