Supplemental Table 1. Proteins Altered >2-fold by Treatment with MLN4924

Gene Name Fold Description Established Change NEDD8/CUL Target

WSB1 34.77 WD repeat and SOCS box-containing protein 1 isoform 2 Yes FOSL 14.51 fos-related antigen 1 Yes CDKN1A 14.44 cyclin-dependent inhibitor 1 Yes TRPC4AP 8.30 short transient receptor potential channel 4-associated Yes protein isoform a GDF15 7.14 growth/differentiation factor 15 CTNNB1 6.93 catenin beta-1 Yes BAHD1 6.58 bromo adjacent homology domain-containing 1 protein MORF4L2 6.05 mortality factor 4-like protein 2 NFE2L2 5.66 nuclear factor erythroid 2-related factor 2 Yes CDT1 5.45 DNA replication factor Cdt1 Yes CDKN1B 4.45 cyclin-dependent kinase inhibitor 1B Yes FBXO11 4.30 F-box only protein 11 Yes MRFAP1 4.13 MORF4 family-associated protein 1 Yes HMOX1 4.07 heme oxygenase 1 MORF4L1 3.51 mortality factor 4-like protein 1 isoform 2 Yes C19orf33 3.49 immortalization up-regulated protein TP53 3.41 tumor suppressor p53 Yes MAFF 3.23 transcription factor MafF isoform a RHOB 3.16 rho-related GTP-binding protein B ETV3 3.06 ETS translocation variant 3 isoform 1 SMARCC1 2.81 SWI/SNF complex subunit SMARCC1 LOC728026 2.80 PREDICTED: hypothetical protein LOC728026 GCLM 2.67 glutamate--cysteine regulatory subunit BRD2 2.63 bromodomain-containing protein 2 EPHA2 2.62 ephrin type-A receptor 2 CDK4 2.60 cyclin-dependent kinase 4 Yes DTL 2.60 denticleless protein homolog Yes GPR4 2.54 G-protein coupled receptor 4 ETNK1 2.40 ethanolamine kinase 1 isoform A KEAP1 2.36 kelch-like ECH-associated protein 1 Yes SET 2.35 protein SET isoform 2 RBM18 2.33 RNA-binding protein 18 LRRC41 2.32 leucine-rich repeat-containing protein 41 DAPK1 2.28 death-associated 1 Yes UBE2J2 2.24 ubiquitin-conjugating E2 J2 isoform 2 Yes CLSPN 2.23 claspin isoform 1 Yes SMARCD1 2.22 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 isoform a ORC6 2.22 origin recognition complex subunit 6 FAU 2.20 ubiquitin-like protein fubi and ribosomal protein S30 precursor DGCR8 2.16 microprocessor complex subunit DGCR8 isoform 2 SLC30A1 2.14 zinc transporter 1 CDCA3 2.12 cell division cycle-associated protein 3 Yes DNAJB4 2.12 dnaJ homolog subfamily B member 4 RBAK 2.11 RB-associated KRAB zinc finger protein SMTN 2.11 smoothelin isoform c KRT10 2.11 keratin, type I cytoskeletal 10 POLR2A 2.09 DNA-directed RNA II subunit RPB1 USP11 2.09 ubiquitin carboxyl-terminal 11 HIST1H1E 2.09 histone H1.4 ATAD2 2.07 ATPase family AAA domain-containing protein 2 LOC650157 2.07 PREDICTED: peptidyl-prolyl cis-trans A- like TXNRD1 2.05 thioredoxin reductase 1, cytoplasmic isoform 1 E2F3 2.04 transcription factor E2F3 Yes SMARCE1 2.04 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 KCTD5 2.04 BTB/POZ domain-containing protein KCTD5 RPL22L1 -2.00 60S ribosomal protein L22-like 1 ELOVL5 -2.00 elongation of very long chain fatty acids protein 5 APOA2 -2.04 apolipoprotein A-II preproprotein HIST1H4J -2.04 histone H4 HIST1H3F -2.04 histone H3.1 H1F0 -2.04 histone H1.0 HBB -2.27 hemoglobin subunit beta RPL14 -2.44 60S ribosomal protein L14 FADS2 -2.44 fatty acid desaturase 2 PCOLCE -2.94 procollagen C-endopeptidase enhancer 1 HIST1H1C -2.94 histone H1.2 HISTH1B -4.34 histone H1.5 ERGIC1 ->100 endoplasmic reticulum-Golgi intermediate compartment protein 1

Table Legend: A2780 human ovarian cancer cells were treated with 1 µM MLN4924 for 24 hours. Protein levels were determined as described in the Materials and Methods. Table includes all proteins whose levels were altered by 2 or more fold following treatment with MLN4924.