Monoclonal CD154 (CD40L)

PN IM1842 – Purified – Freeze-dried – 0.2 mg – Clone TRAP1

For Research Use Only. Not for use in diagnostic procedures.

SPECIFICITY PROCEDURE The ligand for CD40 (CD154 or REAGENT CONTENTS Flow Cytometry: Suggested CD40L), also called TRAP (TNF This reagent is provided in freeze- working dilution: 1:20. Use 10 µL of Related Activation ), is a 33 dried form from phosphate-buffered 5 kDa membrane glycoprotein, that dilution to label 5 x 10 cells. saline (PBS: 0.008 M sodium belonging to the TNF / NGF ligand phosphate, 0.15 M sodium chloride, For other applications it is family (1, 2). Like TNF, it potentially pH 7.2), with 1 mg/mL bovine serum recommended that the user titrates exists both in a membrane- albumin (BSA). the antibody for use in her / his own anchored and a soluble form (1). system using appropriate negative CD154 is undetectable on freshly STATEMENT OF WARNINGS / positive controls. isolated peripheral blood 1. Specimens, samples and all mononuclear cells (3, 4). It is material coming in contact with SELECTED RESEARCH predominantly expressed by them should be handled as if REFERENCES activated CD4+ T cells, although a capable of transmitting infection 1. Graf, D., Korthäuer, U., Mages, proportion of CD8+ cells also and disposed of with proper H.W., Senger, G., Kroczek, expresses the protein (4). CD154 precautions. R.A., "Cloning of TRAP, a ligand induces proliferation and 2. Never pipet by mouth and avoid for CD40 on human T cells", immunoglobulin secretion (4, 5). contact of samples with skin and 1992, Eur. J. Immunol., 22, CD40 / CD154 system is predicted mucous membranes. 3191-3194. to function in the process of B cell 3. Do not use reagent beyond the 2. Fanslow, W.C., Rousseau, A- selection in the germinal centre (6). expiration date on the vial label. M.C., Lofton, T.E., Klinke, R., CD154 is also expressed on 4. Avoid microbial contamination of Ulrich, D.T., Armitage, R.J., activated and triggers an reagent or erroneous results "CD154 (CD40 ligand) inflammatory reaction of endothelial may occur. Workshop Panel report", 1997, cells (7). 5. Use Good Laboratory Practices Leucocyte Typing VI, White Cell The TRAP-1 monoclonal antibody (GLP) when handling this Differentiation . was assigned to the CD154 cluster reagent. Kishimoto, T., et al., Eds., of differentiation at the 6th Garland Publishing, Inc., 101- International Workshop on Human STORAGE CONDITIONS AND 103. Leucocyte Differentiation Antigens STABILITY 3. Hermann, P., Blanchard, D., de in Kobe, Japan, in 1996 (2). Saint-Vis, B., Fossiez, F., This reagent is stable up to the Gaillard, C., Vanbervliet, B., REAGENT expiration date on the vial label Brière, F., Banchereau, J., Monoclonal Antibody CD154 when stored at 2 – 8°C. The Galizzi, J.P., "Expression of a PN IM1842 – Purified – Freeze- reconstituted form may be stored at 32 kDa ligand for the CD40 dried -20°C until the expiration date. on activated human T CLONE TRAP1 Aliquotting is suggested to avoid lymphocytes", 1993, Eur. J. ISOTYPE IgG1 multiple freeze-thaw cycles. The Immunol., 23, 961-964. IMMUNOGEN Mouse myeloma addition of sodium azide at 0.1% 4. Lane, P., Traunecker, A., line transfected with a (w/v) is recommended for storage of Hubele, S., Inui, S., TRAP cDNA the reconstituted form for up to one Lanzavecchia, A., Gray, D., fragment month at 2-8°C. Minimize exposure "Activated human T cells HYBRIDOMA P3X63Ag.8653 x to and warmth. express a ligand for the human Balb/c spleen cells REAGENT PREPARATION B cell-associated antigen CD40 SPECIES Mouse Reconstitute with 1 mL of distilled which participates in - SOURCE ascites fluid water. No preservative has been dependent activation of B PURIFICATION Ion added. lymphocytes", 1992, Eur. J. exchange or affinity Immunol., 22, 2573-2578. chromatography

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Monoclonal Antibody CD154 (CD40L)

PN IM1842 – Purified – Freeze-dried – 0.2 mg – Clone TRAP1

5. Brugnoni, D., Airo, P., Graf, D., 1995, J. Immunol., 154, 5746- Outside the USA, contact your local Marconi, M., Molinari, C., Braga, 5756. Beckman Coulter representative. D., Malacarne, F., Soresina, A., 7. Henn, V., Slupsky, J.R., Gräfes, www.beckmancoulter.com Ugazio, A.G., Cattaneo, R., M., Anagognostopoulos, I., Kroczek, R.A., Notarangelo, Förster, R., Müller-Bergahaus, TRADEMARKS Beckman Coulter and the Beckman L.D., "Ontogeny of CD40 G., Kroczek, R.A., "CD40 ligand Coulter logo are trademarks of expression by activated on activated platelets triggers an Beckman Coulter, Inc. peripheral blood lymphocytes in inflammatory reaction of humans", 1996, Immunol. endothelial cells", 1998, Nature, Letters, 49, 27-30. 391, 591-594. Manufactured by: 6. Lagresle, C., Bella, C., Daniel, Immunotech, a Beckman Coulter OTHER FORMS AVAILABLE P.T., Krammer, P.H., Defrance, Company CD154-PE (IM2216) T., "Regulation of germinal 130, avenue de Lattre de Tassigny, center B cell differentiation", For additional information in the B.P. 177 USA, call 800-526-7694. 13276 Marseille Cedex 9, France

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