R&D Systems Tools for Cell Biology Research™

IHC Products & Protocol Guide IHC & ICC cover images using R&D Systems primary antibodies.

Delta-like 1 (DLL1) Wnt-2 Tie-2 in Embryonic Rat Stomach in Human Stomach Cancer in Embryonic Zebrafish

Catalog # AF3970 Catalog # AF3464 Catalog # AF928 Olig2 and Oligodendrocyte Vanilloid R1 in Rat Dorsal Marker O4 in Rat Cortical IL-2 Receptor a Root Ganglion Stem Cells (RCSC) in Mouse Spleen

Catalog # AF3066 Catalog # AF2418/MAB1326 Catalog # AF2438 STAT6 in Human Notch-2 Peripheral Blood Glutathione Peroxidase 1 in Rat Choroid Plexis Mononuclear Cells (PBMCs) (GPX1) in Rat Liver

Catalog # AF1190 Catalog # MAB2167 Catalog # AF3798 MESDC2 Meprin b Subunit in Adult Mouse Testis in Mouse Intestine GFAP in Rat Astrocytes

Catalog # AF4545 Catalog # AF3300 Catalog # AF2594

To view our continuously updated IHC/ICC image gallery, please visit our website at www.RnDSystems.com/go/IHCimages TABLE OF CONTENTS

This guide provides an introduction to the techniques, protocols, and troubleshooting methods that lead to a successful immunohistochemistry (IHC) or immunocytochemistry (ICC) experiment. Contributors include scientists from R&D Systems IHC/ICC core facility who have experience qualifying thousands of antibodies for these applications, and R&D Systems expert technical service team who have guided researchers through a range of IHC/ICC-related issues.

INTRODUCTION...... 2-10 IHC/ICC PROTOCOLS...... 11-20 Designing a Successful IHC/ICC Experiment...... 2 A. Making a 4% Formaldehyde Solution in PBS ...... 12

Sample Preparation...... 3 B. Preparation of Gelatin-coated Slides Paraffin-embedded & Frozen Tissues for Histological Tissue Sections...... 13 Cell Samples C. Chromogenic Staining of Tissue Sections Fixation...... 4 Using HRP-DAB Detection...... 14-15 Formaldehyde, Alcohols, & Acetone Fixation of Tissues Slide Preparation...... 14 Fixation of Cultured Cells Chromogenic Staining Using HRP-DAB Detection ...... 15 Preventing Non-specific Staining...... 5 D. IHC Staining of Tissue Sections Using Fluorescence Microscopy. . . 16 Preventing Non-specific Hydrophobic Interactions Preventing Non-specific Ionic Interactions E. Antigen Retrieval Using Heat-induced Epitope Retrieval (HIER) . . . 17 Endogenous Enzyme Interference Endogenous Biotin Interference F. Preparation of Coverslips & Cell Fixation for ICC...... 18 Primary Antibody Selection & Optimization...... 6 G. Fluorescent ICC Staining of Cultured Cells...... 19 Selecting a Primary Antibody Supplier H. Preparation of a Cell Smear for Non-adherent Cell ICC...... 20 Selecting a Monoclonal or Polyclonal Antibody Affinity Purification of Polyclonal Antibodies TROUBLESHOOTING GUIDE ...... 21 Optimization of Primary Antibody Incubation ALPHABETICAL LISTING Detection & Visualization...... 7-8 OF R&D SYSTEMS IHC/ICC ANTIBODIES...... 22-108 Direct or Indirect Detection, & Amplification Fluorescence Detection SUPPLEMENTAL IHC/ICC REAGENTS...... 109-112 Chromogenic Detection IHC/ICC Controls...... 9 Endogenous Tissue Background Control No Primary Antibody Control Isotype Control Absorption Control Tissue Type Control Limitations of Western Blot Comparisons Antigen Retrieval ...... 10 Antigen Retrieval Techniques Protease-induced Epitope Retrieval (PIER) Heat-induced Epitope Retrieval (HIER) Optimization & Limitations

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Designing a Successful IHC/ICC Experiment Immunohistochemistry (IHC) and immunocytochemistry (ICC) are techniques employed to localize antigen expression and are dependent on specific epitope- antibody interactions. IHC refers to the use of tissue sections, whereas ICC describes the use of cultured cells or cell suspensions. In both methods, positive staining is visualized using a molecular label, which can be fluorescent or chromogenic. Briefly, samples are fixed to preserve cellular integrity, and then subjected to incubation with blocking reagents to prevent non-specific binding of the antibodies. Samples are subsequently incubated with primary and secondary antibodies, and the signal is visualized for microscopic analysis. Technically, IHC/ICC are relatively simple and straightforward experimental methods. However, there are many variables which must be recognized and optimized for each individual IHC/ICC study. The most challenging aspect of these techniques is determining the experimental conditions necessary to generate a strong and specific signal for each antigen of interest. For example, detection of an abundant protein in cultured cells may require a short fixation period, minimal blocking, and may be compatible with direct visualization using a flurochrome-conjugated primary antibody. In contrast, detection of a phosphorylation-dependent epitope in a section of frozen tissue may require antigen retrieval and be dependent on amplified chromogenic visualization. This guide provides information on the IHC/ICC variables listed below and is intended to assist in the design, optimization, and troubleshooting of IHC/ICC experiments.

VARIABLES FOR EXPERIMENTAL DESIGN & OPTIMIZATION VARIABLE FACTORS A. B. Antigen Species, expression levels, sample types, & subcellular location Epitope Dependence on conformation or post-translation modification Sample Type Tissue or cells Tissue: embedded or frozen Sample Preparation Cells: adhered cell cultures or cell suspensions Appropriate Controls No primary antibody, isotype control, absorption control, tissue type control Fixation Method Perfusion or immersion (with or without freezing)

Fixative* Formaldehyde, alcohols, or acetone Examples of Fluorescence Detection by IHC & ICC. A. Fluorescence detection by IHC was used to visualize V-set and Ig domain-containing protein 1 (VSIG1) in a frozen section of human Blocking Reagent* Normal serum, bovine serum albumin (BSA), or non-fat milk testis using anti-human VSIG1 affinity-purified polyclonal antibody (Catalog # AF4818). Tissue was stained using NorthernLights-557 anti-sheep IgG secondary antibody (Catalog # NL010; Antigen Retrieval* Protease-induced Epitope Retrieval (PIER) or Heat-induced Epitope Retrieval (HIER) red) and counterstained with DAPI (blue). B. Fluorescence detection was used to measure Glial Fibrillary Acidic Protein (GFAP) expression in differentiating cultures of rat cortical stem cells Detection Method Direct or indirect (with or without amplification) using anti-human GFAP affinity-purified polyclonal antibody (Catalog # AF2594). The cells were Primary Antibody* Monoclonal or polyclonal stained using anti-sheep NorthernLights-557 secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue). Secondary Antibody* Species and label Labeling Method Fluorescence or chromogenic A. B. Fluorochromes and spectral properties Label Chromogens: 3,3' Diaminobenzidine (DAB), 3-amino-9-ethylcarbazole (AEC) Fluorescence: 4,6'-diamidino-2-phenylindole (DAPI) Counterstain* Chromogenic: hematoxylin Fluorescence: anti-fade mounting medium Mounting Reagent Chromogenic: aqueous mounting medium Visualization & Analysis Fluorescence microscope or standard light microscope

* These variables require optimization of additional factors including concentration, pH, temperature, incubation time, and diluent. Examples of Chromogenic Detection by IHC & ICC. A. Chromogenic detection of Interferon Regulator Transcription Factor 8 (IRF8) expression in paraffin-embedded human lymphoma tis- This table is not intended to be comprehensive but represents the most commonly applied IHC/ICC methods at R&D Systems. sue section using anti-human IRF8 affinity-purified polyclonal antibody (Catalog # AF5117). The tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue). B. Chromogenic detection of IFN-g ex- pression in human peripheral blood mononuclear cells, stimulated with calcium ionomycin (0.5 mg/mL) and PMA (50 ng/mL), using anti-human IFN-g affinity-purified polyclonal antibody (Catalog # AF285). The cells were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

2 For research use only. Not for use in diagnostic procedures. INTRODUCTION

Sample Preparation Tissue and cell samples must be appropriately harvested and prepared for each IHC/ICC study. To facilitate the required incubation steps, whole tissues must be cut into ultra thin (5-10 mm) sections or cut into smaller pieces for whole mount IHC. For ICC experiments, cells must be attached to a microscope slide or coverslip before commencing the staining procedure. Sample preparation is also intimately linked to the method of fixation, which in turn is influenced by the desired detection technique (fluorescence versus chromogenic).­ In most circumstances, one experimental variable will determine the most appropriate method of sample preparation. For example, tissue that is immersion-fixed in formaldehyde must be paraffin-embedded and cut using a microtome. Alternatively, to detect a phosphorylation- dependent epitope, tissue may need to be snap frozen, which requires a cryostat for sample sectioning followed by alcohol fixation.

Paraffin-embedded Tissue IHC SAMPLE PREPARATION COMPARISON Paraffin embedding offers the best option for long-term preservation of TISSUE tissue samples. Tissue must be fixed before being embedded in paraffin. Paraffin-embedded Frozen Fixation can be achieved by perfusion or immersion immediately following FIXATION Pre-embedding Post-sectioning dissection, and typically requires 4-24 hours. It is not recommended to fix SECTIONING Microtome Cryostat tissue for more than 24 hours because overfixation can cause masking of STORAGE Multiple years at room temperature 1 year at - 80 ˚C the antigen (see page 10). If necessary, tissues can be transferred to alcohol after fixation until there is time for the embedding process. ADVANTAGES Preserves tissue morphology Preserves enzyme and antigen function Formation of ice crystals may Because paraffin is immiscible with water, tissue must be dehydrated be­ LIMITATIONS Overfixation can mask the epitope negatively affect tissue structure fore adding molten paraffin wax. Dehydration is achieved by immersion in increasing concentrations of alcohol. This approach allows for a gradual Cell Samples change in hydropho­bicity and minimizes cell damage. Following To perform ICC studies, the cells must first be attached to a solid support, dehydration, the tissue is incubated with xylene to clear any remaining such as a microscope slide or coverslip. This is simple with adherent cells, ethanol. Paraffin is typi­cally heated to 60 ˚C for embedding and is which can be grown directly on high-grade, sterilized coverslips placed on subsequently allowed to harden overnight. The tissue is subsequently cut the bottom of a 6- or 24-well plate. Cell adhesion can be enhanced by pre- with a sharp blade into ultra thin slices using a microtome. Sections are coating the coverslip with a charged polymer like Poly-L-Lysine and/or an then dried onto micro­scope slides and can be stored at room temperature extracellular matrix protein such as Laminin, Fibronectin, or Collagen. All for extended periods of time. Tissue sections must be rehydrated before steps of the staining process can be performed with the coverslip in the commencing the IHC/ICC protocol (see page 14). Paraffin is the cheapest microplate by adding the appropriate solution to each well and removing and most commonly used substance for tissue embedding. However, solutions by aspiration. Cells in suspension can be adhered to a slide by tissue can also be embedded in plastic, which sets harder and allows Cytospin® centrifugation or by a 10 minute incubation of the cells on thinner tissue sections (1.5 mm versus 5 mm). slides that have been coated with Poly-L-Lysine. Frozen Tissue Note: It is important to be able to recognize artifacts of poorly prepared samples in order to properly interpret results. For example, folds or tears One of the benefits of frozen tissue samples is the time saved by omitting in the tissue, or bubbles between the tissue/cells and the slide, can collect the initial fixation step required for paraffin-embedded tissue. Snap staining reagents non-specifically and obscure positive staining or lead to freezing can be particularly beneficial when detecting post-translational false-positive signals. modifications such as phosphorylation. Frozen tissue can be prepared by immersing the tissue in liquid nitrogen or isopentane, or by burying it in dry ice. For frozen samples, a short fixation is done after freezing and sectioning. Frozen tissue sec­tions are most often fixed using alcohol, which avoids the possible requirement to retrieve epitopes masked by formaldehyde cross-linking. Frozen tissue is cut on a cryostat and sections can be stored at - 80 ˚C for up to 1 year. Frozen tissue sections can be processed in a shorter amount of time than paraffin-embedded sections. However, freezing is not adequate for long- term preservation of the tissues and the formation of ice crystals within the cells may negatively affect subcellular detail. In addition, frozen sections are often thicker than paraffin sections. This can increase the potential for lower microscopic resolution and poor images of tissue morphology. Because frozen tissue often retains enzymatic activities, blocking the functions of endogenous enzymes that may affect the IHC detection method is also particularly important (see page 5).

Cytospin is a registered trademark of Thermo Electron Corporation.

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Fixation All samples used in IHC/ICC experiments must be fixed to preserve tissue morphology and retain the antigenicity of the target molecules. Fixation alters the chemical composition of tissues and often requires a compromise between preserving tissue structure and preserving the epitope. Incomplete fixation (underfixation) of cells or tissues may allow rapid proteolytic degradation of target proteins within the tissue and can reduce specific immunoreactivity. However, excessive fixation (overfixation) may result in masking of the epitope or in strong non-specific background staining that can obscure specific labeling. As discussed in the previous section, the method and timing of fixation must be considered in terms of sample preparation. In addition, duration, temperature, and pH will all affect the degree of fixation.

Formaldehyde Acetone Formaldehyde is the most common fixative used to preserve protein Acetone is a strong dehydrating agent, which can cause irreversible pre­ targets within tissues and cells. Formaldehyde-mediated tissue fixation is cipitation of tissue proteins. It is usually used on sections of unfixed, snap- thought to be dependent on the formation of protein-protein and protein- frozen tissues, followed by fixation with alcohols or formaldehyde. nucleic acid cross-links involving methylene bridges (-CH2-). Formaldehyde can chemically link NH (amino) and CONH (peptide) groups, NH and NH, 2 2 Fixation of Tissues or NH and NH groups. 2 2 Whole animal perfusion fixation is often the best way to preserve antigens Formaldehyde is a good choice for most IHC/ICC applications but is not when studying intact tissues of small animals such as mice, rats, and a universal fixative. Overfixation with formaldehyde can modify the amino guinea pigs. It involves replacing the animal’s systemic blood with a acids that are part of an epitope and can block an antibody from binding fixative solution. However, whole animal perfusion may not be sufficient to it. However, in most cases the epitope can be unmasked to restore to fix the tissues of interest. In cases such as these, dissected tissues can be antibody bind­ing using antigen retrieval techniques (see page 10). immersed in the fixative. For example, 4% formaldehyde in PBS is a Formaldehyde has been shown to induce intracellular translocation of commonly used solution for perfusion and immersion fixation of tissues. phosphorylation-dependent epitopes from the membrane to the cytoplasm. In situations like this, ice cold absolute methanol or absolute To enhance penetration of the fixative during immersion fixation, it is ethanol are appropriate alternative fixatives. recommended that tissues be no thicker than 10 mm. For complete fixa­ ­tion, the volume of the fixative should be 50-100 times larger than the volume of Note: Although there are many different fixatives available, formaldehyde the tissue. Fixation is usually achieved at room temperature for 4-24 hours. is the most commonly used, and is an appropriate starting point for most It is very important to optimize fixation conditions since underfixation or IHC/ICC experiments. Formaldehyde-based solutions should be aliquoted overfixation may reduce or abolish tissue immunoreac­tivity. and frozen, or stored at 4-8 ˚C for no longer than one month. See page 12 for a protocol for making a typical 4% formaldehyde solution. Fixation of Cultured Cells Alcohols In contrast to tissue samples, cultured cells can be fixed for a shorter period of time with a less concentrated fixative solution. For example, fix­ The most popular alcohols used for cell and tissue fixation are methanol ation with a 2% formaldehyde solution for 20 minutes at room tempera­ and ethanol. The molecular structures of methanol and ethanol closely ture is often sufficient to preserve both cellular morphology and resemble that of water. Therefore, they compete with water for protein antigenicity. hydrogen bonds, replacing water molecules in tissues. This causes pro­ teins to precipitate at their isoelectric points by reducing their dielectric Fixation of cultured cells is usually achieved by simply discarding the constant, and can block antibody-epitope binding due to changes in culture medium and adding the fixative solution. However, the change in conformation. While alcohols affect the tertiary structure of proteins by surface tension following removal of the culture medium can damage breaking hydrophobic bonds, it appears that they can stabilize the some cell types. If this is the case, the fixative can be added directly into secondary structure of proteins. the culture medium. For example, adding the same volume of 4% form­ aldehyde as the volume of culture medium will result in a 2% formalde­ However, it is generally thought that alcohols do not preserve tissue hyde solution, which is strong enough to pre-fix the cells. After 2 minutes, morphology as well as formaldehyde-based fixatives. Alcohols do not the pre-fixation culture medium should be replaced with a fresh volume penetrate as well as formaldehyde and are primarily used to fix frozen of 2% fixative. The pre-fixation step makes the cells more rigid so they can tissue sections and cells. Consequently, alcohol fixation is more suitable for withstand any potential deleterious effects created by changes in surface membrane surface antigens. Antigen retrieval is not recommended tension. following alcohol fixation as it is generally considered too harsh and will likely compromise the integrity of the tissue sections or cells. Note: Fixa­tion can result in hydrophobic cross-linking of tissue proteins. The time, temperature, pH, and fixative used will determine the degree of cross-linking. Once the fixation protocol has been optimized, the same pro­cedure should be used consistently.

4 For research use only. Not for use in diagnostic procedures. INTRODUCTION

Preventing Non-specific Staining Once the tissue or cell samples have been appropriately prepared and fixed, the samples are ready to be stained. All IHC/ICC studies are dependent on specific antibody-epitope binding, which is governed by hydrophobic interactions, ionic interactions, hydrogen bonding, and other intermolecular forces. However, the same attractive forces can also result in non-specific staining, i.e. binding of the primary antibody to amino acids other than those within the desired epitope of the antigen. This is a common problem that occurs in IHC/ICC experiments. The challenge is to reduce non-specific interactions without impairing antibody-epitope binding. Causes of non-specific staining include interactions of the primary and secondary antibodies with serum proteins, ionic interactions between antibodies and tissues, and interactions with endogenous molecules capable of affecting the IHC detection system used. These issues can result in high background and an inability to visualize the antigen of interest in its appropriate cellular location. Staining problems of this type can be addressed by blocking non-specific interactions using a blocking reagent. These steps are performed prior to incubation of the sample with the primary antibody.

Preventing Non-specific Hydrophobic Interactions Although hydrophobic interactions play an essential role in epitope- negatively impact staining specificity. Due to their single epitope speci­ antibody binding, these forces can also promote non-specific binding. ficity, increasing ionic strength is more likely to impair monoclonal than Most proteins have some degree of hydrophobicity due to the neutral polyclonal antibody performance. side chains of several amino acids. Tissue incubation with heat-inactivated Endogenous Enzyme Interference normal serum or bovine serum albumin (BSA) is a common procedure used to reduce non-specific hydrophobic binding. Selection of the type of Chromogenic detection methods often use a conjugated enzyme to vis­ normal serum is important to prevent interactions with the primary or ualize epitope-antibody interactions. When using this method of detection, secondary antibodies, or with the tissues/cells being stained. For example, the endogenous activity of the same enzyme must be blocked. For example, goat serum would not be advised as a blocking reagent for use with a protocols that include horseradish peroxidase (HRP) or alkaline phospha­ goat-derived primary antibody. Instead, a serum identical to the host tase (AP) may require reagents to prevent non-specific signals. Tissues such animal of the secondary antibody or from an unrelated species is as kidney, liver, or vascular areas with red blood cells, contain endogenous recommended. BSA and non-fat dry milk are also frequently used as peroxidase activity. Peroxidase blocking reagents formulated with 3-10% blocking reagents. One of these reagents is typically included in the diluents H2O2 can be used to prevent endogenous peroxidase from cleaving the for the primary and secondary antibodies. The addition of non-ionic substrate. Endogenous AP found in intestine, kidney, lymphoid and other detergents including 0.3% Triton X-100™ or Tween 20™ can also reduce non- tissue can be blocked with 1 mM Levamisole. The intestinal form of AP is specific hydrophobic interactions. unaffected by Levamisole but can be blocked by using 1% acetic acid. A. B. A. B.

Blocking Non-specific Binding with Serum. A. CD14 was detected in paraffin-embedded hu- Quenching Endogenous Peroxidase Activity. A. Failing to quench endogenous peroxidase man tonsil tissue using anti-human CD14 biotinylated affinity-purified polyclonal antibody prior to staining produced a false positive signal in sections of human kidney. Tissue was (Catalog # BAF383). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval stained using the anti-goat HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS008; brown). B.

Kit (Catalog # CTS013). Tissue was stained using high sensitivity streptavidin conjugated to HRP Endogenous peroxidase activity was quenched in the same tissue using 3% H2O2 in methanol (HSS-HRP) and DAB, and counterstained with hematoxylin (blue). B. Non-specific background for 15 minutes at room temperature prior to staining. staining is markedly reduced in a parallel experiment which included a blocking step using animal serum for 15 minutes at room temperature prior to incubation with the primary anti- Endogenous Biotin Interference body. HSS-HRP and animal blocking serums are included in all R&D Systems Cell and Tissue Staining Kits (see page 112 for available kits). Streptavidin binding to a biotinylated primary or secondary antibody is another common detection system that is utilized for IHC/ICC experiments Preventing Non-specific Ionic Interactions (see page 7). Therefore endogenous biotin must be blocked before strepta­ ­ If the antibody employed and the target tissue have net opposite charge, vidin incubation. Endogenous biotin is found in many tissues including liver, ionic interactions can cause non-specific background staining. For ex­ kidney, heart, brain, and lung. Pre-incubation of the sample with avidin is ample, non-specific staining may be due to attraction between opposing routinely used to block endogenous biotin. This must be followed by a sub­ carboxyl and amino groups. Van der Waals forces, weak electrostatic sequent incubation with biotin to block additional biotin binding sites on the interactions between dipolar molecules, can also contribute. Increasing avidin molecule. Following these biotin/avidin blocking steps, the sample can the ionic strength of the fixative and/or the antibody diluent buffer can be incubat­ed with the primary antibody. reduce ionic interactions. However, epitope-antibody binding is common­ ly dependent on ionic forces, so this approach also has the potential to

Triton X-100 is a trademark of Dow Chemical Corporation. Tween is a trademark of ICI Americas.

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Primary Antibody Selection & Optimization The most important factor when designing an IHC/ICC experiment is selection of the primary antibody. In turn, the critical feature of a primary antibody is specificity for the epitope. All steps of an IHC/ICC experiment must be optimized to visualize specific staining and minimize non-specific background signals. This includes performing initial studies to determine the appropriate incubation conditions for each primary antibody. The working dilution for an antigen affinity-purified polyclonal antibody is generally lower than that of a monoclonal antibody but these values must be determined empirically. To achieve a robust and specific signal, a high quality antibody that exhibits minimal cross-reactivity should be employed.

Selecting a Primary Antibody Supplier When searching for a primary antibody, it is likely that products will be reduce background staining, and produce more consistent results com­ available from several different commercial sources that may offer varying pared to non-purified antibodies. degrees of antibody quality. This initial choice can make the difference Monoclonal Antibody Binding Polyclonal Antibody Binding between a successful experiment and a missed opportunity. A first step is to look for independent verification. This includes checking for prior successful use of the antibody in the literature. Many commercial suppliers, including R&D Systems, can provide assistance to researchers looking for this information. Another potential question to ask is whether the antibody is actually manufactured by the supplier or is it purchased and resold? Purchasing direct ensures the commercial source has had complete control of manufacturing, quality control processes, and shipping and Monoclonal and Polyclonal Antibody Binding. Monoclonal antibodies bind only a single storage conditions. In addition, the manufacturer may be in the best epitope, while polyclonal antibodies bind different epitopes on the same protein, position to provide quality technical service should questions arise about the use of the antibody. Optimization of Primary Antibody Incubation Primary antibody concentration, diluent, incubation time, and temperature Selecting a Monoclonal or Polyclonal Antibody all impact the quality of staining. These variables need to be optimized for The inherent features of monoclonal and polyclonal antibodies are as­ each antibody and sample to achieve specific staining and low background. sociated with advantages and limitations when applied to IHC/ICC. Mono­ Often optimization is approached by maintaining a constant incubation clonal antibodies, produced from a single B cell clone, represent a homo­ time and temperature, while varying the antibody concentration to deter­ geneous population that bind with high affinity and specificity to a single mine when an optimal signal is achieved with low background noise. For epitope. This can be particularly useful when detecting a single member example, if a high affinity antibody is used, then the antibody can be used of a protein family that shares a high percentage of amino acid identity. at a relatively high concentration for a shorter incubation time. Alter­natively, a high affinity antibody at lower antibody concentration may be used with Antibody binding is often dependent on the target protein maintaining its a longer incubation time. Longer incubation durations are often employed native conformational state. Access to a desired epitope can be com­ to ensure penetration throughout tissue sections used for stereological promised by interactions with other proteins, post-translational modifica­ techniques.­ To promote specific staining, longer incubation periods are tions, temperature, pH, fixation, and salt concen­tration. Because polyclonal often conducted at lower temperatures (i.e. 4 ˚C versus room temperature). antibodies are heterogeneous and can recognize multiple epitopes, they are less likely to be affected by changes in protein conformation. In general, Optimization of R&D Systems staining protocols for tissue sections typi­ polyclonal antibodies are also more stable than monoclonal antibodies over cally begin with an overnight incubation with the primary antibody at 4 ˚C. a range of pH and salt concentration. For these reasons, polyclonal anti­ For staining cells, a 1 hour incubation with the primary antibody at room bodies are more frequently used for IHC/ICC experiments than monoclonal temperature is a common starting point. The working dilution of an anti­ antibodies. gen affinity purified polyclonal antibody is generally lower (from 1.7-15 mg/mL) than that of a monoclonal antibody (5-25 mg/mL). When comparing samples stained with different concentrations of the same antibody, it is Affinity Purification of Polyclonal Antibodies im­portant to use a consistent time and temperature for the incubation Polyclonal antiserum contains a mixture of antibodies produced by a large step. When working with a new antibody for the first time, it is recom­ number of B cell clones. Antibodies comprising polyclonal antiserum bind mended to conduct preliminary studies testing a broad range of antibody to target epitopes with different specificities and affinities, as well as cross- concentrations. react with irrelevant target molecules (non-specific interactions). To enrich for antibodies with the highest specificity and affinity toward the antigen STARTING CONDITIONS FOR PRIMARY ANTIBODY INCUBATION of interest, R&D Systems polyclonal antibodies are antigen affinity purified. ANTIBODY SAMPLE During this process polyclonal antiserum is passed through an affinity Monoclonal Polyclonal column containing immobilized antigen molecules. Specific antibodies Tissue 5-25 mg/mL, overnight at 4 ˚C 1.7-15 mg/mL, overnight at 4 ˚C are retained by the immobilized antigen, whereas non-specific anti­bodies Cells 5-25 mg/mL, 1 hour at room temperature 1.7-15 mg/mL, 1 hour at room temperature pass through the column and are discarded. The retained antigen affinity Advantage Single epitope specificity Lower concentration required purified antibodies are subsequently eluted from the column. Antigen af­ finity purified antibodies interact predominantly with their target antigen, Limitation Vulnerable to epitope masking Heterogeneous population

6 For research use only. Not for use in diagnostic procedures. INTRODUCTION

Detection & Visualization Following incubation with the primary antibody, antibody binding is visualized using an appropriate detection system. The method of detection can be direct or indirect, and may generate a fluorescent or chromogenic signal. Direct detection involves the use of primary antibodies that are directly conjugated to a label. Indirect detection methods utilize a labeled secondary antibody raised against the primary antibody host species. Indirect methods can also include amplification steps to increase signal intensity. Commonly used labels for the visualization of epitope-antibody interactions include fluorophores and enzymes that convert soluble substrates into insoluble, chromogenic end products. The choice of label is affected by the detection method employed, personal preference, and the type microscope equipment that is available

Direct or Indirect Detection, & Amplification DETECTION METHODS & SIGNAL INTENSITY The choice of whether to use direct or indirect detection is often dictated DIRECT by the level of antigen expression. For example, detection of a highly expressed epitope might be possible using a primary antibody directly conjugated to a label. Advantages of direct detection include the ease of use for multicolor staining and the elimination of concerns regarding non- specific binding of the secondary antibody. The major disadvantage is that direct detection may lack the sensitivity required to visualize lower ADVANTAGE LIMITATION expression levels. In rare cases the conjugation process may also have a Good for multicolor labeling Lower sensitivity negative impact on the affinity of the primary antibody.

In contrast, indirect detection methods generally have a higher level of INDIRECT sensitivity and generate a more intense signal. The signal is amplified us­ing the indirect method because of the potential for at least two labeled secondary antibodies to bind to each primary antibody. However, the use of a secondary antibody requires extra blocking steps and additional controls. Further amplification of the signal can be achieved by taking advantage of the strong affinity of avidin and streptavidin to bind biotin. Avidin is a glycopro­tein in egg white that combines stoichiometrically with biotin. ADVANTAGE LIMITATION Streptavidin is purified from the bacterium Streptomyces avidinii, is not Higher sensitivity Potential non-specific binding of secondary antibodies glycosylated, and exhibits lower non-specific binding than avidin. Both proteins bind four biotins per molecule. If a biotinylated secondary anti­ body is employed, the signal can be significantly amplified by subsequent AMPLIFICATION – ABC METHOD incubation with an avidin-biotin complex (ABC Method), or labeled streptavidin-biotin (LSAB Method). Streptavidin may be conjugated­ to a detection enzyme (i.e. horseradish peroxidase or alkaline­ phosphatase) or fluorochrome. Using these amplification methods requires additional steps to prevent non-specific binding (see page 5).

ADVANTAGE LIMITATION Increased signal intensity Requires additional time & controls

KEY: Antigen AMPLIFICATION – LSAB METHOD Primary Antibody

Secondary Antibody

Label

Avidin/Streptavidin

Biotin ADVANTAGE LIMITATION Increased signal intensity Requires additional time & controls

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Fluorescence Detection Fluorescence detection is based on the use of fluorochromes that emit A. light when excited by light of a shorter wavelength. The fluorochrome can be conjugated directly to the primary or secondary antibody, or to streptavidin. Immunofluorescence is commonly used for the simultaneous visualization of multiple cellular targets. For example, tissues can be incubated with a mixture of different primary antibodies followed by incubation with secondary reagents conjugated to fluorochromes that emit light at different wavelengths. Emission Intensity Multicolor experiments must be designed to limit cross-reactivity between the detection reagents and crossover between the spectral properties of the fluorochromes being used. To limit cross-reactivity between secondary 400 450 500 550 600 650 700 reagents, the primary antibodies should be derived from different species. Wavelength (nm) This allows for the use of species-specific secondary antibodies that recognize only one primary antibody. However, there may be some B. instances when it is necessary to simultaneously use antibodies from the same species. This can be achieved using a biotinylated form of one of the primary antibodies. When using this technique, tissues must be incubated with the non-biotinylated antibody first, followed by incubation with the fluorophore-conjugated secondary antibody. The tissues are then incubated with the biotinylated primary antibody followed by incubation with a streptavidin-conjugated fluorochrome. This approach will ensure that the streptavidin conjugate will only bind the biotinylated antibody, limiting cross-reactivity.

R&D Systems NorthernLights™ Fluorochromes have Non-overlapping Spectral Proper­ ties that make them Ideal for Multicolor IHC and ICC. A. Emission curves for Northern- Detection Lights-493 anti-goat IgG (Catalog # NL003; green) and NorthernLights-557 anti-mouse IgG (Catalog # NL007; red). B. Multicolor detection of Nestin and b-III Tubulin in rat cortical stem cells (Catalog # NSC001) using anti-rat Nestin affinity-purified polyclonal antibody (Catalog # Emission Filter AF2736; green) and anti-neuron specific b-III Tubulin monoclonal (clone TuJ-1) antibody (Cata- log # MAB1195; red). Cells were stained with NorthernLights-493 (Catalog # NL003; green) and NorthernLights-557 (Catalog # NL007; red) secondary antibodies and counterstained with DAPI (blue). Dichroic Mirror TYPICAL FLUORESCENCE Excitation FILTER SET Chromogenic Detection Light Source To facilitate chromogenic detection, the primary antibody, secondary anti­ Excitation Filter body, or streptavidin is conjugated to an enzyme. When a soluble organic substrate is applied, the enzyme reacts with the substrate to generate an Sample insoluble colored product that is localized to the sites of antigen expression. Commonly used enzymes include horseradish peroxidase (HRP) and al­kaline phosphatase, which convert 3,3' diaminobenzidine (DAB) and 3-amino-9-ethylcarbazole (AEC), into brown and red end products,­ re­ spectively. DAB is more commonly used than AEC since the latter is soluble in alcohol and is more prone to fade when exposed to excessive light. Multicolor Fluorescence Microscopy. A typical immunofluorescence setup that depicts the Aqueous counterstain and mounting media must be used with AEC. light source, a suitable filter set for the fluorochrome of interest, and a detection mechanism. Chromogenic detection is considered to be more sensitive than that of immunofluorescence, but is less convenient because it includes more incubation and blocking steps. Like immunofluorescence, chromogenic detection allows for the visualization of multiple antigens, but only if the antigens are confined to different locations in the cell and tissue because overlapping colors may obscure results. An advantage of DAB chromogen­ ic staining is that the colored precipitate formed during the reaction between HRP and DAB is not sensitive to light and the slides can be stored for many years. Unlike fluorescence microscopy, which requires a specialized light source and filter sets, chromogenic techniques require only a typical NorthernLights is a trademark of R&D Systems. light microscope.

8 For research use only. Not for use in diagnostic procedures. INTRODUCTION

IHC/ICC Controls Appropriate controls are critical for the accurate interpretation of IHC/ICC results. A satisfactory IHC/ICC experimental design produces results that demonstrate that the antigen is localized to the correct specialized tissues, cell types, or subcellular location. Optimization of fixation, blocking, antibody incubation, and antigen retrieval steps will generate a strong and specific signal. However, IHC/ICC experiments must include positive and negative controls to support the validity of staining and identify experimental artifacts. In addition, variances in antibody specificity, experimental conditions, biological conditions between tissue types, and even researchers may generate inconsistent staining and lead to inaccurate conclusions. To facilitate consistent performance, detailed record keeping is an important factor for IHC/ICC studies. Here, we describe several established controls that can be used to support the specificity of your IHC/ICC results.

Endogenous Tissue Background Control Certain cells and tissues may have inherent biological properties resulting The antigen to antibody mixture should be made at a working dilution of in background staining that could lead to a misinterpretation of the re­ 10:1 (molar ratio) and be pre-incubated overnight at 4 ˚C. The pre-absorbed sults. Before applying primary antibodies, cells and tissues should be in­ antibody can then be incubated with tissue in place of the primary spected under the microscope using either fluorescence (for fluores­cent antibody alone. The staining pattern produced by the primary antibody­ labels) or bright-field (for chromogenic labels) illumination to ensure there can be compared to that produced by the pre-absorbed antibody. is no signal inherent to the tissue itself. For instance, lipofuscin is an Absorption controls work better if the immunogens are peptides. However, endogenous autofluorescent pigment that can be confused with positive if antibodies were raised against the whole protein, addition of the mixture staining. of antibody plus protein may result in higher non-specific staining. A. B. C. Although the mechanism is unclear, the antigen used for pre-absorption may itself bind to the tissue and result in non-specific staining. Thus, it is important to note that an absorption control using whole protein may not always confirm the specificity of an antibody for the protein in the tissue. A. B.

Lipofuscin Background in Nervous System Tissue. Lipofuscin is a pigment that accumulates with age in many tissue types. It also has autofluorescent properties that overlap with the exci- tation and emission spectra of commonly used fluorochromes. Circled in the micrographs above are lipofuscin-containing neurons that may appear labeled using either bright-field microscopy (A) or fluorescence microscopy in the green (B) and red spectrums (C).

No Primary Antibody Control A control in which the tissue is incubated with antibody diluent, without Absorption Control in Rat Dorsal Root Ganglion. A. A cryostat section of rat dorsal root ganglion stained for phospho-MSK1 (S212) using anti-human Phospho-MSK1 (S212) affinity the primary anti­body included, is always necessary. This is followed by purified polyclonal antibody (Catalog # AF1036). B. Nuclear staining (indicated by arrows) is incubation with secondary antibodies and detection reagents. Staining abolished if the antibody is first pre-absorbed with the S212 phosphorylated immunogen. with detection reagents alone should be negligible to the point that it does not obscure specific staining or resemble the specific staining pattern. Tissue Type Control Additional controls for IHC/ICC experiments include using tissue samples Isotype Control that are known to express (or not express) the epitope of interest. This strategy can provide a useful reference and may also be utilized during This control can be utilized when working with monoclonal primary initial optimization studies. Tissue from transgenic animals that overexpress antibodies. The sample is incubated with antibody diluent, supplemented or do not express the antigen can be particularly useful. In addition, tissue with a non-immune immuno­globulin of the same isotype (for example, samples from different species can be included to support the species- IgG , IgG , IgG , IgM) and concentration as the primary monoclonal anti­ 1 2A 2B specificity of an antibody. body. The sample is then incubated with the secondary antibody and de­ tection reagents. These steps will help ensure that what appears to be spe­cific staining was not caused by non-specific interactions of immuno­ Limitations of Western Blot Comparisons globulin molecules with the sample. Background staining should be neg­ Western blot experiments are often conducted to complement and ligible and not resemble specific staining. support IHC/ICC studies. However, changes in protein conformation Absorption Control during denaturation can result in potentially misleading results. For a given antibody, inconsistent results between Western blot and IHC/ICC To demonstrate that an antibody is binding specifically to the antigen of studies may simply reflect differences in the experimental conditions interest, it is first pre-incubated with the immunogen. This should employed. Since multiple epitopes are recognized, polyclonal antibodies inactivate the antibody and the tissue should show little or no staining. are less vulnerable to such experimental artifacts.

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Antigen Retrieval Although fixation is essential for the preservation of tissue morphology, this process can also have a negative impact on IHC/ICC detection. Fixation can alter protein biochemistry such that the epitope of interest is masked and can no longer bind to the primary antibody. Masking of the epitope can be caused by cross-linking of amino acids within the epitope, cross-linking unrelated peptides at or near an epitope, altering the conformation of an epitope, or altering the electrostatic charge of the antigen. Antigen retrieval refers to any technique in which the masking of an epitope is reversed and epitope-antibody binding is restored.

Antigen Retrieval Techniques The need for antigen retrieval depends on multiple variables, including artifactual staining should always be considered when using any antigen but not limited to, the target antigen, the antibody used, the type of tissue, retrieval methodology. The use of controls to demon­strate specific anti­ and the method and duration of fixation. Because they recognize multiple body binding­ should be included since staining is influenced by multiple epitopes, polyclonal antibodies are more likely than monoclonal antibod­ variables in any given experiment. ies to detect­ a given antigen without the use of antigen retrieval techniques. ANTIGEN RETRIEVAL SOLUTION pH TIME There are multiple techniques to restore the immunoreactivity of an Acidic Neutral Basic epitope. Methods as simple as changing the pH or the cation concentration 1 minute Slide #1 Slide #2 Slide #3 of the antibody diluent can influence the affinity of an antibody for its 5 minutes Slide #4 Slide #5 Slide #6 epitope. For partially masked epitopes it may be appropriate to first try increasing the primary antibody incubation conditions before commen­ 10 minutes Slide #7 Slide #8 Slide #9 cing antigen retrieval. However, this step will also require further op­ Optimization of HIER. A typical experimental set-up to determine optimum HIER incubation timization in terms of appropriate antibody concentration, incubation time and pH. Results should be compared to a tenth slide, with no HIER. A similar approach can be employed to optimize incubation temperature. time, and temperature. When discussing antigen retrieval methods, tech­ niques generally fall into two main categories, protease-induced epitope R&D Systems offers reagents to improve tissue antigen detection and retrieval (PIER) and heat-induced epitope retrieval (HIER). Once optimized, enhance immunoreactivity. During initial optimization, investigators can the effects of antigen retrieval can be pronounced. compare samples treated with neutral pH 7.0 Universal Antigen Retrieval Solution (Catalog # CTS015) with a slide of the same tissue without antigen Protease-induced Epitope Retrieval (PIER) retrieval. Subsequent tests using acidic or basic antigen retrieval solutions In the PIER method, enzymes including Proteinase K, Trypsin, and Pepsin may be necessary depending on the tissue. At R&D Systems, we find that have been used successfully to restore the binding of an antibody to its treatment with Basic Antigen Retrieval reagent (Catalog # CTS013) is epitope. The mechanism of action is thought to be the cleavage of pep­ frequently successful. We also offer Acidic (Catalog # CTS014) and Sampler tides that may be masking the epitope. The disadvantages of PIER are the (Catalog # CTS016) Antigen Retrieval Reagents. Acidic and basic antigen low success rate for restoring immunoreactivity and the potential for retrieval solutions are more likely to affect tissue morphology than a destroying both tissue morphology and the antigen of interest. neutral solution. No HIER Acidic HIER Heat-induced Epitope Retrieval (HIER) HIER is believed to reverse some cross-links and allows for restoration of secondary or tertiary structure of the epitope. The protocol must be opti­ mized for each tissue, fixation method, and antigen to be studied. In gen­ eral, HIER has a much higher success rate than PIER. HIER is performed using microwave ovens, pressure cookers, vegetable steamers, autoclaves, or water baths. Microwaves are an increasingly pop­ ular appliance for HIER. These protocols tend to involve 5 minute periods of heat followed by replacement of the buffer. For all HIER methods, slides Neutral HIER Basic HIER must be cooled before commencing IHC/ICC incubations. HIER is especially time-, temperature-, buffer-, and pH-sensitive, and the best method must be determined empirically.­

Optimization & Limitations Antigen retrieval may require increased adherence of the sample to the slide or coverslip. In addition, the technique is often too harsh for cryostat tissue sections and alcohol-fixed tissue. The time, temperature, and pH must also be optimized for each appliance (i.e. 5-10 minutes at 92-95 °C Antigen Retrieval Improves Detection of p27. IHC images show the detection of p27 in paraffin- embedded human prostate cancer sections following incubation of tissue for 10 minutes at 95 ˚C in a water bath, versus 1-5 minutes at 120 °C in a pressure cooker). To in the specified antigen retrieval solution. Compared to no HIER treatment, p27 detection was optimize antigen retrieval preliminary studies must be conducted using enhanced following incubation in neutral (pH 7.0) and basic (pH 9.5) (Catalog # CTS013) but not a matrix of time, temperature, and pH combinations. The possibility of acidic (pH 5.0) (Catalog # CTS014) antigen retrieval solution. p27 was detected using anti- human/mouse/rat p27 monoclonal antibody (Catalog # MAB22561; brown).

10 For research use only. Not for use in diagnostic procedures. IHC/ICC PROTOCOLS IHC/ICC PROTOCOLS

Immunohistochemistry Immunocytochemistry

BLOCK PRIMARY ANTIBODIES SECONDARY ANTIBODIES LABEL COUNTERSTAIN COVERSLIP

RAGE in Alzheimer’s Brain. Meteorin in Rat Cortical Stem Cells.

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A. Making a 4% Formaldehyde Solution in PBS The vast majority of IHC/ICC procedures employ fixation of tissues and cells using formaldehyde-based fixatives. The protocol below describes the technique for generating a 4% formaldehyde solution in PBS. The most effective fixative must be determined­ experimentally. Caution: Formaldehyde is toxic, read the MSDS before working with this chemical. Gloves and safety glasses should be worn, and solutions made inside a fume hood. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED REAGENTS

. Deionized H2O . HCl (Dilute) 40 g Paraformaldehyde Powder . NaOH (1 N) . Paraformaldehyde powder 1 N NaOH UNTIL POWDER DISSOLVES . PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4 MATERIALS . Filter units . Glassware and stir bar (Dedicated for formaldehyde solution) . Gloves and eye protection . Hot plate with magnetic stirrer 800 mL 1x PBS AT ≈ 60 ˚C HEAT WHILE STIRRING . Thermometer . Ventilated hood

PROCEDURE 1. For 1 L of 4% Formaldehyde add 800 mL of 1x PBS to a glass beaker on a stir plate in a ventilated hood. Heat while stirring to approximately 60 ˚C. Take care that the solution does not boil. 2. Add 40 g of paraformaldehyde powder to the heated PBS solution. COOL & FILTER WITH FILTER PAPER 3. The powder will not immediately dissolve into solution. ADJUST THE VOLUME TO 1 L Slowly raise the pH by adding 1 N NaOH dropwise from a pipet WITH 1x PBS until the solution clears. 4. Once the paraformaldehyde is dissolved the solution should be cooled and filtered. 5. Adjust the volume of the solution to 1 L with 1x PBS. 6. Recheck the pH and adjust it with small amounts of dilute HCl to approximately 6.9. 7. The solution can be aliquoted and frozen, or stored at 4 ˚C for ADJUST THE pH TO 6.9 WITH DILUTE HCl no longer than one month. ALIQUOT & FREEZE

H H OH H O 2 The difference between paraformaldehyde, formaldehyde, & formalin. Paraformaldehyde (chemical C O C name is polyoxymethylene) is a powder of polymerized formaldehyde that by itself cannot fix tissues. To H H OH be usable as a tissue fixative, paraformaldehyde has to be dissolved in hot water to become a formalde- hyde solution. Formalin is a saturated formaldehyde solution in water (37% by weight, 40% by volume) Formaldehyde Methylene Glycol containing 10-15% methanol. Methanol is added to slow down the poly­merization of formaldehyde, Formaldehyde Structure. In an aqueous solution, formaldehyde exists which reduces the fixing power of formalin. Formalin can also be made in an alcohol-free form from primarily as methylene glycol. powdered paraformaldehyde.­

12 For research use only. Not for use in diagnostic procedures. IHC/ICC PROTOCOLS

B. Preparation of Gelatin-coated Slides for Histological Tissue Sections In order for tissue sections to be retained on histological slides during staining and washing steps, slides need to be coated with adhesive compounds. Although there are a variety of such compounds, gelatin is the most frequently used for histological­ purposes. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED 5 g Gelatin REAGENTS AFTER THE GELATIN DISSOLVES ADD 0.5 g CrK(SO4)2 . Gelatin-coating solution: 1 L deionized H2O, 5 g gelatin, 0.5 g chromium potassium

sulfate CrK(SO4)2 MATERIALS . Filter units . Histological slides . Hot plate with magnetic stirrer . Slide racks 1 L Deionized H20 HEAT AT 45 ˚C WHILE STIRRING . Staining dish . Thermometer

PROCEDURE 1. Prepare the gelatin-coating solution by dissolving 5 g of gelatin

in 1 L of heated, deionized H2O (temperature should not exceed 45 ˚C).

2. After the gelatin has dissolved, add 0.5 g of chromium COOL & FILTER AT ROOM TEMPERATURE potassium sulfate. Chromium potassium sulfate will positively charge the slides allowing them to attract negatively charged tissue sections. 3. Filter this solution and store at 2-8 ˚C until use. It is ADD Gelatin-coating Solution recommended that this solution be filtered again immediately before use (adjust to room temperature before filtration).

4. Place the histological slides into metal racks. DIP RACKS WITH SLIDES 3 to 5 TIMES, Note: The slides should be cleaned by washing them in soapy water 5 SECONDS EACH and rinsing them thoroughly, first in tap water and finally in deionized water.

5. Dip the racks containing the slides 3 to 5 times (~5 seconds Staining Dish each) into the gelatin-coating solution. 6. Remove the racks containing the slides and let them drain. Blot excess solution from the racks onto filter paper (gently tap the racks against the filter paper for better drainage). Histological Slides 7. Place the racks containing the slides on the lab bench and cover them with paper towels to protect them from dust. 8. Dry at room temperature for 48 hours. 9. Dried slides can be put back into the boxes that they arrived in DRY AT ROOM TEMPERATURE and stored at room temperature until use. Slides intended for FOR 48 HOURS cryostat sections can be stored at -20 ˚C.

STORE AT ROOM TEMPERATURE OR AT -20 ˚C IF INTENDED FOR CRYOSTAT SECTIONS

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C. Chromogenic Staining of Tissue Sections Using HRP-DAB Detection The following protocol has been developed and optimized by R&D Systems IHC/ICC laboratory using the HRP-DAB Cell and Tissue Staining Kits (see page 112). Protocols may need to be modified depending on the type of tissue used. Each investigator should determine the optimal working dilution of an antibody. If using R&D Systems primary antibodies, refer to the product data sheets to obtain approximate working dilutions. For all other reagents follow the manufacturer’s instructions. Caution: DAB is hazardous. Gloves and safety glasses should be worn, and all steps performed inside a fume hood. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED REAGENTS If necessary, perform antigen retrieval (see page 17 for protocol). . Aqueous Mounting Medium (Catalog # CTS011, or equivalent) . Primary Antibodies If endogenous peroxidase is present in the tissue . Cell and Tissue Staining Kit: (See page 112) 3. Incubate the sample with 1-3 drops peroxidase blocking reagent Anti-goat (Catalog # CTS008), Anti-mouse (Catalog # CTS002), for 5 minutes Anti-rabbit (Catalog # CTS005), Anti-rat (Catalog # CTS017) Kit contents: Peroxidase Blocking Reagent, Avidin Blocking Reagent, Biotin Blocking Note: This procedure may affect the morphology of unfixed Reagent, Biotinylated Secondary Antibodies, High Sensitivity Streptavidin-HRP frozen tissue sections. Reduction in the incubation time may be Conjugate (HSS-HRP), DAB Chromogen Solution, Serum Blocking Reagents required. . Deionized H 0 2 4. Rinse the sample, then gently wash in wash buffer for 5 minutes. . Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton™ X-100, and 0.01% sodium azide 5. Surround the tissue with a hydrophobic barrier using a barrier pen.

. PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4 Proceed to the staining protocol on page 15. . Wash buffer: PBS (1x) . For Paraffin-embedded tissue sections : Xylenes (mixed isomers) 100% denatured alcohol CRYOSTAT SECTIONS PARAFFIN-EMBEDDED TISSUE SECTIONS 95% denatured alcohol 70% denatured alcohol THAW SLIDES Xylene 100% 95% 70% 50% 50% denatured alcohol AT ROOM Alcohol Alcohol Alcohol Alcohol TEMPERATURE OPTIONAL REAGENTS 10-20 MINUTES . Antigen retrieval reagents (see page 17) . DAB Enhancer (Catalog # CTS010) MATERIALS . Coverslips 2 times 2 times 1 time 1 time 1 time . Hydrophobic barrier pen 10 minutes 10 minutes 5 minutes 5 minutes 5 minutes . Slides with paraffin-embedded or cryostat tissue sections RINSE WITH Deionized H20 PROCEDURE REHYDRATE THE SLIDES 1. Slide preparation for Cryostat Sections WITH Wash Buffer FOR 5 MINUTES a. When staining cryostat sections stored in a freezer, thaw the slides at room temperature for 10-20 minutes. DRAIN EXCESS Slide preparation for Paraffin-embedded Tissue Sections Wash Buffer AND PERFORM a. Immerse the slides in xylene (mixed isomers) ANTIGEN RETRIEVAL 2 times for 10 minutes each. IF NECESSARY b. Immerse the slides in 100% alcohol 2 times for 10 minutes each. c. Immerse the slides in 95% alcohol for 5 minutes. d. Immerse the slides in 70% alcohol for 5 minutes. e. Immerse the slides in 50% alcohol for 5 minutes.

f. Rinse the slides with deionized H20. 2. Rehydrate the slides with wash buffer for 10 minutes. Drain the excess wash buffer. If proceeding directly to one of the staining protocols on pages 15 or 16, surround the tissue with a hydrophobic barrier using a barrier pen.

14 For research use only. Not for use in diagnostic procedures. IHC/ICC PROTOCOLS

Chromogenic Staining Using HRP-DAB Detection

PROCEDURE 1. Block the slides with 1-3 drops of serum blocking reagent for 15 BLOCKING (FOLLOW STEPS 1-3) minutes. Drain the slides and wipe away any excess blocking reagent before proceeding to the next step. Do not rinse. 2. Incubate the sample with 1-3 drops of avidin blocking reagent for 15 minutes. Rinse the sample with wash buffer, drain slides, and wipe away any excess wash buffer. 3. Incubate the sample with 1-3 drops of biotin blocking reagent for 15 minutes. Rinse with wash buffer, drain the slides, and wipe RINSE WITH Wash Buffer, away any excess wash buffer. DRAIN SLIDES 4. Incubate the sample with primary antibodies in dilution buffer. Follow manufacturer’s recommendations regarding working ADD Primary Antibodies dilution, incubation time, and temperature. These variables may INCUBATE THE SAMPLE ACCORDING TO THE need to be modified for your system. MANUFACTURER’S RECOMMENDATIONS 5. Rinse the sample with wash buffer and then wash 3 times in wash buffer for 5 minutes each and drain the slides. RINSE 3 TIMES FOR 5 MINUTES EACH WITH Wash Buffer, AND DRAIN 6. Incubate the sample with 1-3 drops of biotinylated secondary antibodies for 30-60 minutes, adjusting the incubation time depending on the thickness of the section (approximately 30 ADD μ μ Biotinylated Secondary minutes for 5-10 m thick sections and 60 minutes for 10-20 m Antibodies thick sections). INCUBATE FOR 30-60 MINUTES 7. Rinse the sample with wash buffer then wash 3 times in wash buffer for 15 minutes each and drain the slides. 8. Incubate the sample with 1-3 drops of HSS-HRP for 30 minutes. RINSE 3 TIMES FOR 15 MINUTES EACH WITH Wash Buffer, AND DRAIN 9. Rinse the sample with wash buffer then wash 3 times in wash buffer for 2 minutes each. ADD 10. Calculate the working volume of DAB Chromogen Solution given HSS-HRP INCUBATE FOR that 100-200 μL is required to cover the entire tissue section on 30 MINUTES a slide. Add 1-5 drops of DAB Chromogen Solution to cover the en­tire tissue section and incubate for 3-20 minutes. Monitor the inten­sity of the tissue staining under a microscope. RINSE AND WASH 3 TIMES IN Wash Buffer FOR 2 MINUTES EACH Note: DAB Enhancer can be used to intensify the DAB Chromogen solution. DAB is hazardous. Refer to the MSDS for safe deactivation. ADD DAB Chromogen Solution 11. Rinse the sample with wash buffer 3 times for 10 minutes each. INCUBATE 3-20 MINUTES MONITORING INTENSITY 12. Rinse in deionized H20 and drain the slides. OF TISSUE STAINING UNDER A MICROSCOPE 13. Stained tissue can be mounted either without counterstaining or counterstained with hematoxylin for better visualization of the tissue morphology. RINSE 3 TIMES FOR 10 MINUTES EACH WITH Wash Buffer 14. Cover stained tissue with a coverslip of an appropriate size. & 1 TIME WITH Deionized H2O Place slides vertically on a filter paper or towel to drain excess COUNTERSTAIN mounting medium and allow them to dry. IF REQUIRED MOUNT WITH 15. Visualize tissues under a light microscope. Aqueous Mounting Medium

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D. IHC Staining of Tissue Sections for Fluorescence Microscopy The following protocol has been developed and optimized by R&D Systems IHC/ICC laboratory using the NorthernLights fluorescent secondary reagents (see page 110). Protocols may need to be modified depending on the type of tissue used. Each investigator should determine the optimal working dilution of an antibody. If using R&D Systems primary antibodies, refer to the product data sheets to obtain approximate working dilutions. For all other reagents follow the manufacturer’s instructions. Caution: DAB is hazardous. Gloves and safety glasses should be worn, and all steps performed inside a fume hood. Please read the protocol in its entirety before starting.

ADDITIONAL SUPPLIES FOR FLUORESCENT IHC STAINING REAGENTS 5. Incubate for 1 hour at room temperature with a NorthernLights- . Primary Antibodies conjugated secondary antibody (or equivalent) in dilution buffer . Anti-fade Mounting Medium designed to recognize the host species of the primary antibody. (i-BRITE Plus, Catalog # SF40000, Neuromics, Inc., or equivalent) Alternatively, a NorthernLights-streptavidin conjugate (or . Blocking buffer: 10% normal donkey serum, 0.3% Triton X-100 equivalent) can be used if the primary antibody is biotinylated. . Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton™ X-100, and 0.01% sodium azide Note: Some tissues may bind non-specifically to biotin-avidin . NorthernLights Fluorescent Secondary Antibodies/Streptavidin Conjugates labeling components resulting in high background staining. (or equivalent) (See page 5)

. PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4 . Wash buffer: PBS (1x) 6. Wash the slides 3 times for 15 minutes each in wash buffer. MATERIALS 7. Mount with an anti-fade mounting medium. . Coverslips BLOCK THE SLIDE WITH . Slides with paraffin-embedded or cryostat tissue sections Blocking Buffer (STEP 2) IHC Staining Using Fluorescence Microscopy

PROCEDURE INCUBATE FOR 15 MINUTES AT 1. Slide preparation (See Step 1 on page 14) ROOM TEMPERATURE 2. Block the slides using blocking buffer for 15 minutes at room temperature and rinse with wash buffer. RINSE WITH Wash Buffer 3. Make working dilutions of the primary antibodies in dilution buffer according to manufacturer’s instructions. Add small ADD Primary Antibodies volumes of these dilutions to the slides and incubate at 4 ˚C overnight, or for 30-60 minutes at room temperature. INCUBATE SAMPLE AT 4 ˚C OVERNIGHT Note: Multiple primary antibodies to different antigens can OR 1 HOUR AT be added in step 5 as long as care is taken to avoid the use of ROOM TEMPERATURE secondary reagents with overlapping spectra. If possible, primary antibodies should also be raised in different species. Please see RINSE 3 TIMES IN Wash Buffer page 8 for more details. FOR 15 MINUTES ADD Secondary Antibodies Note: A negative control and/or an isotype-matched control should be included to identify non-specific binding from the secondary antibody. Please see page 5 for more details about IHC/ICC controls. INCUBATE FOR 1 HOUR AT 4. Wash the slides 3 times for 15 minutes each in wash buffer. ROOM TEMPERATURE

RINSE 3 TIMES IN Wash Buffer FOR 15 MINUTES

MOUNT WITH Anti-fade Medium Triton X-100 is a trademark of Dow Chemical Corporation.

16 For research use only. Not for use in diagnostic procedures. IHC/ICC PROTOCOLS

E. Antigen Retrieval Using Heat-induced Epitope Retrieval (HIER) Antigen retrieval can reveal epitopes masked during the preparation of tis­sues for staining. The protocol below describes a technique used by R&D Systems and can be used on both cryostat and paraffin-embedded sections. The protocol can be used with R&D Systems Acidic, Basic, or Universal Antigen Retrieval Reagents. Pretreatment with these solutions may induce a dramatic enhancement of immunoreactivity. However, they also have the potential to affect tissue morphology. The optimal method of antigen retrieval must be determined experimentally.­ For examples of IHC studies using antigen retrieval, see page 10. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED REAGENTS . Antigen Retrieval Solution (10x): HEAT SLIDES IN Antigen Retrieval Solution Antigen Retrieval Reagent-Basic (Catalog # CTS013) or FOR 2-10 MINUTES Antigen Retrieval Reagent-Acidic (Catalog # CTS014) or 92-95 ˚C Antigen Retrieval Reagent-Universal (Catalog # CTS015)

. Deionized H2O

. PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4 EQUIPMENT . Polypropylene Coplin staining jar (or equivalent) . Water bath at 92-95 ˚C Water bath MATERIALS . Slides with paraffin-embedded or cryostat tissue sections ALLOW TO COOL AT ROOM TEMPERATURE PROCEDURE RINSE WITH 1. Make working dilutions by mixing 1 part of 10x Antigen Deionized H20 AND THEN Retrieval concentrate with 9 parts of deionized water. WITH PBS 2. Preheat retrieval solution to 92-95 ˚C. This may be achieved by AVOID VIGOROUS placing a polypropylene Coplin staining jar filled with retrieval RINSING solution into a water bath. Note: Heating may cause cracking of glass staining dishes. 3. Immerse slides into preheated retrieval solution for 2-10 minutes. Coplin Staining Jar Note: Since the effect of antigen retrieval reagents depends on their temperature (90-100 ˚C) and incubation time (up to 30 minutes), optimal conditions should be determined by the individual investigator. Note: Cryostat sections are more sensitive to damage by retrieval solution than paraffin-embedded tissues. To avoid tissue damage, it may be necessary to shorten the incubation time to 2-5 minutes.

4. After the incubation is finished, remove the Coplin jar with BEGIN STAINING PROCEDURE retrieval solution and slides from the water bath, and let it cool to room temperature. 5. Gently rinse the slides with deionized water and then with PBS. Note: Because tissues may be loosened after the retrieval procedure, avoid vigorous rinsing to prevent detachment from the slides. 6. Tissues are now ready for blocking and primary antibody incubation.

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F. Preparation of Coverslips & Cell Fixation for ICC The fundamental principles of IHC experiments using tissue sections also apply to ICC experiments. However, these protocols must be specially adapted for use with cultured cells or cell suspensions. The following protocols describe how to prepare gelatin-coated coverslips and fix cultured cells for ICC experiments. Please read the protocols in its entirety before starting.

Coverslip Preparation Using Gelatin Preparation & Fixation of Cells on Coverslips Many cultured cell types do not adhere well to glass coverslips. There are SUPPLIES REQUIRED several techniques used to enhance cell adhesion including the use of poly- amino acids such as Polyornithine or Poly-L-Lysine, and/or extracellular REAGENTS matrix proteins such as Laminin or Fibronectin. Adding a thin layer of gelatin . Fixative: 2-4% formaldehyde in PBS to a coverslip is also often used to enhance the adhesion of cultured cells . Wash buffer: 0.1% BSA in 1x PBS . Cell culture medium to glass. . PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4 SUPPLIES REQUIRED MATERIALS REAGENTS . Gelatin-coated coverslips in a 24-well plate . Gelatin-coating solution: 0.1% gelatin in deionized H O 2 PROCEDURE MATERIALS . Coverslips (Sterilized) 1. Culture cells by adding 0.5 mL of culture media containing . Cell culture plate (6- or 24-well) approximately 5,000 cells to the wells of a cell culture plate containing gelatin-coated coverslips. PROCEDURE 2. When cells have reached the desired density/age, remove the 1. Sterilize coverslips and place them into the wells of a 24-well culture media from each well and wash twice with PBS. plate. 3. Add 300-400 µL of 2-4% formaldehyde to each well and 2. Add 400 µL of gelatin-coating solution and incubate the incubate for 20 minutes at room temperature. coverslips for 10 minutes at room temperature. 4. Wash the wells twice with PBS and then cover with 400 µL of 3. Remove the gelatin-coating solution and air dry the coverslips wash buffer. The coverslips can be stored at 2-8 ˚C for up to 3 for 15 minutes. months or they may be stained immediately. 4. The dried coverslips can now be stored at room temperature Note: Fixa­tion can result in hydrophobic cross-linking of tissue until use. proteins. The time, temperature, pH, and fixative used will determine the degree of cross-linking. Once the fixation protocol has been optimized, the same procedure­ should be used consistently.

ADD 4% Formaldehyde ADD 400 µL FOR 20 MINUTES AT of Wash Buffer PLATE CELLS ON CULTURE TO ROOM TEMPERATURE GELATIN-COATED DESIRED COVERSLIPS DENSITY/AGE STAIN OR STORE AT 2-8 °C FOR UP TO WASH 2 TIMES WASH 2 TIMES 3 MONTHS WITH PBS WITH PBS

18 For research use only. Not for use in diagnostic procedures. IHC/ICC PROTOCOLS

G. Fluorescent ICC Staining of Cultured Cells The following protocol has been developed and optimized using R&D Systems primary antibodies and NorthernLights fluorescent secondary antibodies (see page 8). This protocol may need to be optimized for each specific ICC experiment. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED REAGENTS 10. Carefully remove the coverslips from the wells and blot to remove . Primary Antibodies any excess water. Dispense 1 drop of Anti-fade mounting . Blocking buffer: 10% normal donkey serum, 0.3% Triton X-100 medium onto the microscope slide per coverslip. Mount the . DAPI (4',6-diamidino-2-phenylindole) solution: add 1 μL of 14.3 mM stock for every coverslip with the cells facing towards the slide. 5 mL of PBS. Store any unused DAPI at 2-8 ˚C, wrapped in aluminum foil 11. Visualize using a fluorescence microscope and filter sets . Deionized H2O . Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, appropriate for the label used. Slides can also be stored in a slide 0.3% Triton™ X-100, and 0.01% sodium azide box at < -20 ˚C for later examination. . Anti-fade mounting medium . NorthernLights-conjugated secondary reagents (see page 110), or equivalent

. PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4 . Wash and antibody dilution buffer: 0.1% BSA in PBS (1x) BLOCK MATERIALS . Cell-covered coverslips in a 6- or 24-well plate . Fine tweezers RINSE FIXED CELLS WITH Wash Buffer

PROCEDURE INCUBATE SAMPLE 45 MINUTES AT ROOM TEMPERATURE Note: This protocol is optimized for cells grown on coverslips in a 6- or 24-well plate but can be adapted accordingly. ADD Primary Antibodies NO RINSING IS NECESSARY 1. Wash the coverslips or slides containing the fixed cells two times in 400 µL of wash buffer INCUBATE OVERNIGHT AT 2-8 ˚C 2. Add 400 µL of blocking buffer and block for 45 minutes at room OR FOR 1 HOUR temp­erature. AT ROOM TEMPERATURE 3. Remove blocking buffer. No rinsing is necessary. ADD Secondary Antibodies RINSE 2 TIMES WITH Wash Buffer 4. Dilute the unconjugated primary antibody (or fluorescence- conjugated primary) in dilution buffer according to the manufacturer’s instructions. Incubate overnight at 2-8 ˚C, or INCUBATE FOR 1 HOUR alternatively, incubate at room temperature for 1 hour. Note: Experimentation with both antibody concentration and ADD RINSE 2 TIMES WITH Wash Buffer incubation time may be required to achieve optimal labeling. DAPI Counterstain 5. Wash two times in 400 µL of wash buffer. If using a primary antibody with a direct fluorescent conjugate, go to step 8. INCUBATE 2-5 MINUTES AT ROOM TEMPERATURE 6. Dilute the secondary antibody in dilution buffer according to the manufacturer’s instructions. Add 400 µL to the wells and RINSE 1 TIME WITH PBS incubate at room temperature for 1 hour in the dark. From this RINSE 1 TIME WITH Deionized H O step forward samples should be protected from light. 2

7. Wash two times in 400 µL of wash buffer. REMOVE THE COVERSLIP WITH A FINE TWEEZERS 8. Add 300 µl of the diluted DAPI solution to each well and incubate 2-5 minutes at room temperature. DAPI binds to DNA and is a convenient nuclear counterstain. It has an absorption MOUNT WITH THE CELLS maximum at 358 nm and fluoresces blue at an emission FACING THE SLIDE maximum of 461 nm. 9. Wash once with PBS and once with water.

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H. Preparation of a Cell Smear for Non-adherent Cell ICC One method of preparing non-adherent cells for ICC involves smearing the sample across a gelatin- coated slide. This technique forms a monolayer of cells that can be easily visualized. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED REAGENTS . Deionized H O FIX CELLS FOR 20 MINUTES SPIN DOWN CELLS RESUSPEND IN 2 AT ROOM TEMPERATURE FOR 30 SECONDS 1 mL Deionized H O . Fixative: 4% formaldehyde in PBS (1x) 2

. PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4 MATERIALS . Microfuge tube (1.5 mL) . Gelatin-coated microscope slides . Hot plate . Hydrophobic barrier pen . Microfuge

PROCEDURE SPIN DOWN CELLS RESUSPEND IN 1. Fix the cells with 4% formaldehyde for 20 minutes at room FOR 30 SECONDS 200 µL Deionized H2O temperature by adding formaldehyde directly to the culture media (see page 4) and adjust to approximately 1 x 106 cells/mL. 2. Add 1 mL of the cell solution to a 1.5 mL microfuge tube. 3. Spin down the cells for 30 seconds in a microfuge. 4. Pour off the supernatant and resuspend the cell pellet in 1 mL

of deionized H2O. ADD 5 µL Cell Suspension 5. Spin down the cells for 30 seconds in the microfuge. TO GELATIN-COATED SLIDE 6. Pour off the supernatant and resuspend the cell pellet in 200 µL

of deionized H2O. 7. Add 5 µL of the cell suspension to a gelatin-coated slide (3 spots per slide) and smear with the side of a pipette tip. 8. Place the slide on a hot plate (low heat setting) and allow the liquid to evaporate. 9. Check the slide under a microscope and make sure that there SMEAR WITH A PIPETTE TIP are no salt crystals. If salt crystals are observed, wash the slide with water. 10. Surround the cell spot with a hydrophobic barrier using a barrier pen and air dry.

11. The slides can be stored at 2-8 ˚C for up to 3 months or they may HEAT TO EVAPORATE THE LIQUID be stained immediately.

SURROUND CELLS WITH A Hydrophobic Barrier

20 For research use only. Not for use in diagnostic procedures. TROUBLESHOOTING GUIDE

Problem: Lack of Staining Possible Source Test or Action Lack of antigen. Check protein expression by in situ hybridization (in some rare cases translation may be blocked even though mRNA is detected). Antibodies do not work due to improper storage. Follow storage instructions on the datasheet. In general, aliquot antibodies into smaller volumes sufficient to make a working solution for a single experiment. Store aliquots in a manual defrost freezer (-20 to -70 ˚C) and avoid repeated freeze-thaw cycles. Inactive primary or secondary antibodies. Test reporter system independently to assess reagent viability. Inadequate tissue fixation. Try increasing the fixation time or try a different fixative. Tissue overfixation. Reduce the duration of the immersion or post-fixation steps. If immersion fixation cannot be avoided (for example, collection of postmortem tissues or biopsies in pathology lab), antigens may be unmasked by treatment with antigen retrieval reagents. Incompatible secondary and primary antibodies. Use a secondary antibody that will interact with the primary antibody. For example, if the primary antibody was raised in rabbits, use an anti-rabbit secondary antibody. Antigen was destroyed before incubation with the primary antibody. If quenching of endogenous peroxidase was done prior to the addition of primary antibodies, block peroxidase after incubation with the primary antibody. Epitope altered during fixation or embedding procedure. Try restoring immunoreactivity through various antigen retrieval techniques. Embed tissue at 58 ˚C or below. Antigen retrieval was ineffective. Increase the time of treatment or change the treatment solution. Reagents omitted or used in wrong order. Repeat staining and confirm that correct reagents are used and are added in the correct order. Problem: High Background Possible Source Test or Action High concentration of primary and or secondary antibodies. Titer antibody to determine optimal concentration needed to promote a specific reaction of the primary and the secondary antibodies. Hydrophobic interactions of the antibody and proteins in the tissue. Lower the ionic strength of the antibody diluent (particularly monoclonal antibodies respond well to reducing the salt concentration). Non-specific binding of primary and/or secondary reagents to tissues. Use blocking step just prior to primary antibody incubation (we use 1% bovine serum albumin with 10% normal donkey serum, or non-fat dry milk is another option). Non-specific binding of secondary antibody. Use an antibody that has cross-reactive IgG species removed (absorbed against sample species). Tissue dried out. Avoid letting the tissue dry during the staining procedure. Reagents sticking to old or poorly prepared slides. Start over with freshly prepared or purchased slides. Background due to ionic interactions. Increase the ionic strength of the diluent buffer. Problem: Cell/Tissue Morphology Destroyed Possible Source Test or Action Antigen retrieval methods are too harsh. Empirically determine the conditions that preserve tissue morphology while restoring the immuno­reactivity of the antigen. Tissue sections falling off slide. (more common with frozen sections) Increase fixation time. Empirically determine an additional or alternative fixative. Use freshly prepared, adequately charged slides. Tissue section appears torn or folded. Air bubbles under section. Re-cut sections using a sharp blade, or ignore damaged areas when analyzing the results. Poor resolution of tissue morphology Cut thinner tissue sections. Ice crystals may have destroyed morphology of frozen sections. Underfixation has physically damaged the tissue or cells during the staining process Increase fixation time and/or add a post-fixation step. Increase the fixative/tissue ratio. Cut smaller pieces of tissue for more thorough immersion fixation. Autolysis of tissue leading to staining of necrotic debris. Increase the fixation time, ratio. Consider using cross-linking fixative. Problem: Staining is Inappropriate Possible Source Test or Action Fixation method is inappropriate for the antigen. Try a different fixative or increase the fixation time. Antigen retrieval may be inappropriate for this antigen or tissue. Try different antigen retrieval conditions. Electrostatic charge of the antigen has been altered. Try adjusting the pH or cation concentration of the antibody diluent. Delay in fixation caused diffusion of the antigen. Fix tissue promptly. Try a cross-linking fixative rather than organic (alchohol) fixative.

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Human None Polyclonal Goat IgG AF1039 100 µg $355 2B4/CD244/SLAMF4 None Polyclonal Goat IgG AF1050 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1050 50 µg $380 Human None Polyclonal Goat IgG AF838 100 µg $355 4-1BB/TNFRSF9/CD137 Human Biotin Polyclonal Goat IgG BAF838 50 µg $380

4-Hydroxynonenal Multi-species None 198960 Mouse IgG2B MAB3249 100 µg $240

5HT1B Human None 499325 Mouse IgG1 MAB5858 100 µg $240 None Polyclonal Sheep IgG AF4975 100 µg $355 Human

5T4 None 524731 Mouse IgG1 MAB4975 100 µg $240 Mouse None Polyclonal Sheep IgG AF5049 100 µg $355

5T4 in Human Placenta. 5T4 was detected in paraf- fin-embedded human placenta sections using anti- human 5T4 affinity-purified polyclonal antibody (Catalog # AF4975). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Cata- log # CTS013). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxy- lin (blue).

8-oxo-dG None 2E2 Mouse IgG2B 4354-MC-050 50 µL $415 A2B5 Human/Mouse/Rat/Chicken None 105 Mouse IgM MAB1416 100 µg $295

A2B5 in Human differentiating NTera2 Cells. NTera2 neuronal carcinoma cells were differentiated with retinoic acid for 10 days. A2B5 was detected using anti-human/mouse/rat/chicken A2B5 mono- clonal antibody (Catalog # MAB1416). The cells were stained using NorthernLights-557 anti-mouse IgG secondary antibody (Catalog # NL007; red) and the nuclei counterstained with DAPI (blue).

a1-Acid Glycoprotein Human None 386131 Mouse IgG2A MAB3694 100 µg $240 None Polyclonal Goat IgG AF1938 100 µg $355 a-2-Macroglobulin Human Biotin Polyclonal Goat IgG BAF1938 50 µg $380 None Polyclonal Goat IgG AF586 100 µg $355

a2u-Globulin Rat Biotin 129736 Mouse IgG1 BAM586 250 µg $380

None 129736 Mouse IgG1 MAB586 500 µg $325 None Polyclonal Goat IgG AF3080 100 µg $355 Human

A33 None 402104 Rat IgG2A MAB3080 100 µg $240 Mouse None Polyclonal Goat IgG AF2756 100 µg $355

Biotin 5D3 Mouse IgG2B BAM995 250 µg $380 ABCG2 Human

None 5D3 Mouse IgG2B MAB995 500 µg $325 None Polyclonal Goat IgG AF929 100 µg $355 Human Biotin Polyclonal Goat IgG BAF929 50 µg $380 ACE/CD143 None Polyclonal Goat IgG AF1513 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1513 50 µg $380

ACE in Human Kidney. Angiotensin Converting Enzyme (ACE) was detected in paraffin-embedded human kidney tissue sections using anti-human ACE polyclonal antibody (Catalog # AF929). Tissues were stained using the Goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counter- stained with hematoxylin (blue).

22 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF933 100 µg $355 Human Biotin Polyclonal Goat IgG BAF933 50 µg $380 ACE-2

None 171606 Mouse IgG2A MAB933 500 µg $325 Mouse None Polyclonal Goat IgG AF3437 100 µg $355

ACE-2 in Mouse Kidney. Angiotensin Converting Enzyme-2 (ACE-2) was detected in a cryostat section of mouse kidney using anti-mouse affinity-purified ACE-2 polyclonal antibody (Catalog # AF3437; red). The tissue was counterstained green.

Actin Human/Mouse/Rat None Polyclonal Sheep IgG AF4000 100 µg $355 Activin A bA subunit Human/Mouse/Rat None Polyclonal Goat IgG AF338 100 µg $355 Activin C bC subunit Human None Polyclonal Goat IgG AF1629 100 µg $355 None Polyclonal Goat IgG AF637 100 µg $355 Activin RIA/ALK-2 Human

None 71412 Mouse IgG1 MAB637 500 µg $325 Activin RIB/ALK-4 Mouse None Polyclonal Goat IgG AF1477 100 µg $355 Activin RIIA Human None Polyclonal Goat IgG AF340 100 µg $355 Activin RIIB Human None Polyclonal Goat IgG AF339 100 µg $355 ADAM8 Ectodomain Human None Polyclonal Goat IgG AF1031 100 µg $355 ADAM9 Ectodomain Mouse None Polyclonal Goat IgG AF949 100 µg $355 Human None Polyclonal Total Goat IgG AB936 1 mg $355 ADAM10 Ectodomain Mouse None Polyclonal Total Goat IgG AB946 1 mg $355 None Polyclonal Goat IgG AF935 100 µg $355 Human Biotin Polyclonal Goat IgG BAF935 50 µg $380 ADAM15 Ectodomain

None 23G9 Mouse IgG1 MAB935 500 µg $325 Mouse None Polyclonal Goat IgG AF945 100 µg $355

ADAM15 Expression in Mouse Cerebellum. A dis­ integin and mettalloprotease domain 15 (ADAM15) was detected in cryostat sections of mouse cerebellar Purkinje cells using anti-mouse ADAM15 polyclonal antibody (Catalog # AF945; red). The tissue was coun- terstained green.

ADAM23 aa 60-585 Human None 504425 Mouse IgG2A MAB4974 100 µg $240 ADAM23 in Human Spleen. A disintegrin and me- talloprotease domain 23 (ADAM23) was detected in paraffin-embedded human spleen sections using anti-human ADAM23 monoclonal antibody (Catalog # MAB4974). Tissue was subjected to antigen retriev- al using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxy- lin (blue).

ADAM32 aa 17-476 Human None 539220 Mouse IgG1 MAB5380 100 µg $240

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE ADAMTS1 Propeptide Human None Polyclonal Goat IgG AF3079 100 µg $355

ADAMTS1 in Human Placenta. A disintegrin-like and mettaloprotease with thrombospondin motifs (ADAMTS1) was detected in a paraffin-embedded human placenta section using anti-human ADAMTS1 affinity-purified polyclonal antibody (Catalog # AF3079). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

Mouse None Polyclonal Goat IgG AF1119 100 µg $355 Adiponectin/Acrp30 Rat None Polyclonal Goat IgG AF3100 100 µg $355 Adiponutrin/ADPN Human None Polyclonal Sheep IgG AF5208 100 µg $355

ADPN in Human Adipocytes. Adiponutrin (ADPN) was detected in human adipocytes using anti-human ADPN affinity-purified polyclonal antibody (Catalog # AF5208). Cells were stained using Northern- Lights-557 donkey anti-sheep IgG secondary anti- body (Catalog # NL010; red) and the nuclei counter- stained with DAPI (blue).

None Polyclonal Chicken IgY AF1369 100 µg $355 Human

None 189506 Mouse IgG1 MAB1369 100 µg $240 a-Fetoprotein/AFP

Human/Mouse None 189502 Mouse IgG1 MAB1368 100 µg $240 Mouse None Polyclonal Goat IgG AF5369 100 µg $355 None Polyclonal Goat IgG AF1220 100 µg $355 Aggrecan G1-IGD-G2 Domains Human

None 179509 Mouse IgG2B MAB1220 500 µg $325 Human None Polyclonal Goat IgG AF704 100 µg $355 AgRP/ART Mouse None Polyclonal Goat IgG AF634 100 µg $355

AgRP in Mouse Thalamus. Agouti-Related Protein (AgRP) was detected in a cryostat section of mouse thalamus using anti-mouse AgRP monoclonal anti- body (MAB634; red). The tissue was counterstained green.

None Polyclonal Rabbit IgG AF1457 100 µg $285 AIF Human/Mouse/Rat None Polyclonal Sheep IgG AF5824 100 µg $355

a-Internexin Human None 529724 Mouse IgG1 MAB4844 100 µg $240

Akt Pan Specific Human/Mouse/Rat None 281046 Mouse IgG2B MAB2055 100 µg $240 Phospho-Akt (S473) Pan Specific Human/Mouse/Rat None Polyclonal Rabbit IgG AF887 100 µg $285

Akt1 Human None 302407 Mouse IgG1 MAB17751 100 µg $240

Human None 302501 Mouse IgG1 MAB23152 100 µg $240 Akt2 Human/Mouse/Rat None Polyclonal Goat IgG AF23151 100 µg $355

Serum Albumin Human/Mouse None 188835 Mouse IgG2A MAB1455 100 µg $240 None Polyclonal Goat IgG AF1172 100 µg $355 ALCAM/CD166 Mouse Biotin Polyclonal Goat IgG BAF1172 50 µg $380 None Polyclonal Goat IgG AF577 100 µg $355 ALK-7 Rat

Biotin 118311 Mouse IgG2A BAM5771 100 µg $240

24 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

Biotin B4-78 Mouse IgG1 BAM1448 100 µg $240 Alkaline Phosphatase/ALPL Human/Mouse/Rat

None B4-78 Mouse IgG1 MAB1448 100 µg $240 Alkaline Phosphatase and Oct-3/4 in Human BG01V Embryonic Stem Cells. Alkaline Phospha- tase was detected in the human BG01V embryonic stem cell line using anti-human/mouse/rat Alkaline Phosphatase monoclonal antibody (Catalog # MAB1448). Oct-3/4 was detected using anti-human Oct-3/4 polyclonal antibody (Catalog # AF1759). The cells were stained for Alkaline Phosphatase using NorthernLights-557 anti-mouse secondary antibody (Catalog # NL007; pseudocolored green) and stained for Oct-3/4 using NorthernLights-637 anti-goat sec- ondary antibody (Catalog # NL002; red).

AMIGO2 Human None 273610 Mouse IgG3 MAB20801 100 µg $240

AMIGO3 Mouse None 371301 Rat IgG2A MAB2375 100 µg $240 AMIGO3 in Mouse Cerebellum. Amphoterin-in- duced Gene and ORF 3 (AMIG03) was detected in Purkinje neuron cell bodies in a cryostat section of mouse cerebellum using anti-mouse AMIG03 mono- clonal antibody (Catalog # MAB2375).

Human None 498001 Mouse IgG2A MAB3815 100 µg $240 Aminopeptidase N/ANPEP Mouse None Polyclonal Goat IgG AF2335 100 µg $355

Aminopeptidase N/CD13 in Human Liver Cancer. Aminopeptidase N (APN) was detected in paraffin- embedded human liver cancer sections using anti- human APN monoclonal antibody (Catalog # MAB3815). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Cata­log # CTS002; brown) and counterstained with hematoxy- lin (blue).

Aminopeptidase O/ONPEP Human None Polyclonal Goat IgG AF4647 100 µg $355

Aminopeptidase P1/XPNPEP1 Human None 409124 Mouse IgG1 MAB29701 100 µg $240 None Polyclonal Total Goat IgG AB-262-NA 1 mg $355 Human Amphiregulin None Polyclonal Goat IgG AF262 100 µg $355 Mouse None Polyclonal Goat IgG AF989 100 µg $355 AMPKa1 aa 340-550 Human/Mouse/Rat None Polyclonal Goat IgG AF3197 100 µg $355 AMPKa2 aa 340-552 Human/Mouse/Rat None Polyclonal Goat IgG AF2850 100 µg $355 AMPKb1 Human/Mouse/Rat None Polyclonal Goat IgG AF2854 100 µg $355

Androgen R/NR3C4 Human None H7507 Mouse IgG2A PP-H7507-00 100 µg $380 Angiogenin Human None Polyclonal Goat IgG AF265 100 µg $355 None Polyclonal Goat IgG AF923 100 µg $355 Angiopoietin-1 Human

None 171718 Mouse IgG2B MAB923 500 µg $325

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Angiopoietin-2 Human None Polyclonal Goat IgG AF623 100 µg $355

Angiopoietin-2 in Human Gastrointestinal Can- cer. Angiopoietin-2 was detected in paraffin-embed- ded human gastrointestinal cancer sections using anti-human Angiopoietin-2 affinity-purified poly- clonal antibody (Catalog # AF623). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Angiopoietin-3 Mouse None Polyclonal Goat IgG AF738 100 µg $355 Angiopoietin-4 Human None Polyclonal Goat IgG AF964 100 µg $355 Angiopoietin-like 1 Human None Polyclonal Goat IgG AF2204 100 µg $355 Angiopoietin-like 2 Mouse None Polyclonal Goat IgG AF1444 100 µg $355 None Polyclonal Goat IgG AF136 100 µg $355 Angiopoietin-like 3 Mouse Biotin Polyclonal Goat IgG BAF136 50 µg $380

ANP Human None 361101 Rat IgG2B MAB3366 100 µg $240 AP-2g Human None Polyclonal Goat IgG AF5059 100 µg $355 APC C-Terminus Human None Polyclonal Goat IgG AF3695 100 µg $355

APJ Human None 72133 Mouse IgG3 MAB856 500 µg $325 None Polyclonal Goat IgG AF3129 100 µg $355 APLP-1 Human

None 354008 Mouse IgG2B MAB3129 100 µg $240 None Polyclonal Goat IgG AF3556 100 µg $355 Apolipoprotein B/ApoB Human

None 369717 Mouse IgG2B MAB4124 100 µg $240 APP Pan Specific Human None Polyclonal Goat IgG AF1168 100 µg $355

Phospho-APP (T668) Human/Mouse None 304904 Mouse IgG1 MAB2508 100 µg $240 Phospho-Amyloid Precursor Protein (668T) in Hu- man Alzheimer’s Disease Brain. Amyloid precursor protein (APP) phosphorylated at T668 was detected in paraffin-embedded human Alzheimer’s disease brain sections using anti-human phospho-APP mono­clonal antibody (Catalog # MAB2508). Tissue was stained using anti-mouse HRP-DAB Cell and Tis- sue Staining Kit (Catalog # CTS002; brown) and coun- terstained with hematoxylin (blue).

ARC aa 176-195 Human/Mouse/Rat None Polyclonal Rabbit IgG AF636 100 µg $285 None Polyclonal Goat IgG AF4560 100 µg $355 b-Arrestin 1 Human

None 425817 Rat IgG2A MAB45601 100 µg $240 b-Arrestin 2 Human None Polyclonal Goat IgG AF4564 100 µg $355 Human None Polyclonal Goat IgG AF2589 100 µg $355 None Polyclonal Goat IgG AF1085 100 µg $355 Artemin Mouse Biotin Polyclonal Goat IgG BAF1085 50 µg $380

None 185234 Rat IgG2A MAB10851 500 µg $325 Arylsulfatase A/ARSA Human None Polyclonal Goat IgG AF2485 100 µg $355 ASK1 aa 1011-1196 Human None Polyclonal Sheep IgG AF3575 100 µg $355 Phospho-ATF2 (S490/S498) Human/Rat None Polyclonal Rabbit IgG PPS064 100 µL $355 Axl Human None Polyclonal Goat IgG AF154 100 µg $355

26 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Phospho-Axl (Y779) Human None Polyclonal Rabbit IgG AF2228 100 µg $285

Phospho-Axl (Y779) in Human Stomach. Phospho- Axl was detected in paraffin-embedded human stomach sections using anti-human/mouse phos- pho-Axl affinity-purified polyclonal antibody (Cata- log # AF2228). Tissue was stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxy- lin (blue).

Biotin RA3-6B2 Rat IgG2A BAM1217 250 µg $380 B220/CD45R Mouse

None RA3-6B2 Rat IgG2A MAB1217 500 µg $325 None Polyclonal Goat IgG AF140 100 µg $355 Human

None 37711 Mouse IgG1 MAB140 500 µg $325 B7-1/CD80 Mouse None Polyclonal Goat IgG AF740 100 µg $355 None Polyclonal Goat IgG AF1214 100 µg $355 Rat Biotin Polyclonal Goat IgG BAF1214 50 µg $380 Human None Polyclonal Goat IgG AF-141-NA 200 µg $230 B7-2/CD86 None Polyclonal Goat IgG AF1340 100 µg $355 Rat Biotin Polyclonal Goat IgG BAF1340 50 µg $380 B7-H1/PD-L1 Mouse None Polyclonal Goat IgG AF1019 100 µg $355 B7-H2 Mouse None Polyclonal Goat IgG AF158 100 µg $355 None Polyclonal Goat IgG AF1027 100 µg $355 B7-H3 Human Biotin Polyclonal Goat IgG BAF1027 50 µg $380

BACE-1 Ectodomain Human/Mouse None 137612 Mouse IgG1 MAB931 500 µg $325

BACE-2 Ectodomain Human None 391017 Rat IgG2A MAB4097 100 µg $240 Human None Polyclonal Goat IgG AF124 100 µg $355 BAFF/BLyS/TNFSF13B Mouse Biotin Polyclonal Goat IgG BAF2106 50 µg $380

BAI1 Human None 542515 Mouse IgG1 MAB4969 100 µg $240

BAI3 Human None 409633 Mouse IgG1 MAB39651 100 µg $240 BAMBI/NMA Human None Polyclonal Goat IgG AF921 100 µg $355 Bax aa 12-33 Human/Mouse None Polyclonal Rabbit IgG AF820 30 µg $285 Bax Minus C-Terminus Human None Polyclonal Total Goat IgG AB820 400 µg $395 Bcl-2 aa 1-208 Human/Mouse None Polyclonal Goat IgG AF810 100 µg $355 Bcl-w aa 1-172 Human/Mouse None Polyclonal Goat IgG AF8241 100 µg $355

Bcl-xL aa 3-14 Human/Mouse/Rat None YTH-2H12 Mouse IgG2A 2300-MC-100 100 µg $285 None Polyclonal Chicken IgY AF248 100 µg $355 BDNF Human Biotin Polyclonal Chicken IgY BAF248 50 µg $380

None 35928 Mouse IgG1 MAB248 500 µg $325 BDNF in Rat Spinal Cord and Dorsal Root Ganglia. Brain-derived neurotrophic factor (BDNF) was de- tected in rat dorsal root ganglion using anti-human BDNF affinity-purified polyclonal antibody (Catalog # AF248; red) and counterstained green.

Human None Polyclonal Goat IgG AF-261-NA 100 µg $355 Betacellulin/BTC None Polyclonal Goat IgG AF1025 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1025 50 µg $380 bIG-H3 Mouse None Polyclonal Sheep IgG AF2559 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Biglycan Human None Polyclonal Goat IgG AF2667 100 µg $355

Biglycan in Human Skin. Biglycan was detected in paraffin-embedded human skin sections using anti- human Biglycan affinity-purified polyclonal antibody (Catalog # AF2667). Tissue was stained using anti- goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

BIK Human None Polyclonal Sheep IgG AF5474 100 µg $355 BMI-1 Human None Polyclonal Goat IgG AF3334 100 µg $355 BMP-1/PCP Human None Polyclonal Goat IgG AF1927 100 µg $355

Pro BMP-2 Human None 253717 Mouse IgG2A MAB2260 500 µg $325 None Polyclonal Goat IgG AF355 100 µg $355 BMP-2/BMP-4 Human Biotin Polyclonal Goat IgG BAF355 50 µg $380 BMP-3 Human None Polyclonal Goat IgG AF113 100 µg $355 BMP-3b/GDF-10 Human None Polyclonal Goat IgG AF1543 100 µg $355 None Polyclonal Goat IgG AF615 100 µg $355 BMP-5 Human Biotin Polyclonal Goat IgG BAF615 50 µg $380 BMP-6 Human None Polyclonal Goat IgG AF507 100 µg $355

None 164311 Mouse IgG2B MAB3541 500 µg $325 BMP-7 Human

None 164313 Mouse IgG1 MAB3542 500 µg $325 BMP-8 Human None Polyclonal Goat IgG AF1073 100 µg $355

BMP-15/GDF-9B aa 268-392 Human None 317809 Rat IgG2A MAB2925 100 µg $240 BMP-15/GDF-9b in Mouse Ovary. Bone Morphoge- netic Protein-15 (BMP-15) was detected in a frozen section of adult mouse ovary using anti-human BMP- 15 monoclonal antibody (Catalog # MAB2925). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary antibody (red; Catalog # NL013) and counterstained with DAPI (blue).

BMPR-IA/ALK-3 Human None 550607 Mouse IgG2B MAB24061 100 µg $240

BMPR-IB/ALK-6 Human None 477914 Mouse IgG2B MAB5051 100 µg $240 None Polyclonal Goat IgG AF811 100 µg $355 BMPR-II Human Biotin Polyclonal Goat IgG BAF811 50 µg $380

BMPR-II in Mouse Embryo. BMPR-II was detected in a frozen section of E15 mouse ribs using anti-human BMPR-II affinity-purified polyclonal antibody (Catalog # AF811; green).

BNP Precursor Human None 370124 Rat IgG2B MAB36041 100 µg $240 BOC Mouse None Polyclonal Goat IgG AF2385 100 µg $355

28 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Brachyury Human None Polyclonal Goat IgG AF2085 100 µg $355

Brachyury in Differentiated Human Embryonic Brachyury in Embryonic Mouse Notochord. Stem cells. Brachyury was detected in differentiated Brachyury was detected in E9.5 mouse notochord us- human Embryonic Stem cells using anti-human ing anti-human Brachyury affinity-purified polyclonal Brachyury affinity-purified polyclonal antibody (Cata- antibody (Catalog # AF2085). The cells were stained log # AF2085). Cells were stained using an anti-goat using NorthernLights-557 secondary antibody (Cata- secondary antibody (green) and counterstained with log # NL001; red) and counterstained with DAPI DAPI (blue). Courtesy of Dr. Frank Solder from the Na- (blue). tional Institute of Neurological Disorders and Stroke & Stem Cell Unit at NIH.

Brachyury NorthernLights-conjugated Human NL557 Polyclonal Goat IgG NL2085R 500 µL $350 Human None Polyclonal Goat IgG AF2210 100 µg $355 BRCA1

Human/Mouse/Rat None 265402 Mouse IgG2A MAB2210 100 µg $240 Phospho-BRCA1 (S1423) Human None Polyclonal Rabbit IgG AF1386 100 µg $285

BRCA2 Human None 234403 Mouse IgG1 MAB2476 100 µg $240 None Polyclonal Sheep IgG AF4009 100 µg $355 Brevican Human Biotin Polyclonal Sheep IgG BAF4009 50 µg $380 Brk aa 1-70 Human None Polyclonal Goat IgG AF3966 100 µg $355

BSRP-A Mouse None 529805 Rat IgG2A MAB4916 100 µg $240 BSRP-A in Mouse Hippocampus. Brain-specific receptor-like protein A (BSRP-A) was detected in a cryostat section of mouse hippocampus using anti mouse BSRP-A monoclonal antibody (Catalog # MAB4916). The tissue was stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxy- lin (blue).

Human None Polyclonal Goat IgG AF2379 100 µg $355 C1q R1/CD93

Mouse None 223437 Rat IgG2B MAB1696 100 µg $240 C4.4A/LYPD3 Mouse None Polyclonal Goat IgG AF5567 100 µg $355 None Polyclonal Sheep IgG AF2715 100 µg $355

Cadherin-6/KCAD Human None 427909 Mouse IgG1 MAB2715 100 µg $240

None 427903 Mouse IgG3 MAB27151 100 µg $240 K-Cadherin in Human Liver Cancer. K-Cadherin was detected in paraffin-embedded human liver cancer sections using anti-human K-cadherin monoclonal antibody (Catalog # MAB27151). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Cadherin-11 Human None Polyclonal Goat IgG AF1790 100 µg $355

Cadherin-12 Human None 343621 Rat IgG1 MAB2240 100 µg $240

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Cadherin-13 Human None Polyclonal Goat IgG AF3264 100 µg $355

Cadherin-13 in Human Lung Cancer Cells. Cad- herin-13 was detected in NCI-H460 human lung can- cer cell line using anti-human Cadherin-13 affinity- purified polyclonal antibody (Catalog # AF3264). Cells were stained using NorthernLights-557 anti- goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

None Polyclonal Goat IgG AF1032 100 µg $355 Cadherin-17 Human Biotin Polyclonal Goat IgG BAF1032 50 µg $380

None 141713 Mouse IgG1 MAB1032 500 µg $325 None Polyclonal Goat IgG AF648 100 µg $355

Human None 180215 Mouse IgG2B MAB1838 500 µg $325

None 180224 Mouse IgG2B MAB18381 100 µg $240 E-Cadherin None Polyclonal Goat IgG AF748 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF748 50 µg $380

None 114420 Rat IgG2A MAB7481 100 µg $240 E-Cadherin and SOX2 in the BG01V Human Embryonic Stem Cell Line. E-Cadherin and SOX2 were detected in human BG01V cells using anti- human E-Cadherin affinity-purified polyclonal anti- body (Catalog # AF648; red) and anti-human/ mouse SOX2 monoclonal antibody (Catalog # MAB2018; green). Cells were stained using anti-goat NorthernLights-557 (Catalog # NL001) and anti- mouse NorthernLights-493 (Catalog # NL009). Cells were counterstained with DAPI (blue).

N-Cadherin Human None Polyclonal Sheep IgG AF1388 100 µg $355

N-Cadherin in Human Hippocampus. N-Cadherin was detected in a paraffin-embedded section of human hippocampus using anti-human N-Cadherin affinity-purified polyclonal antibody (Catalog # AF1388). The tissue was stained using anti-sheep HRP-DAB detection reagents (brown) and counter- stained with hematoxylin (blue).

Human None 104805 Mouse IgG1 MAB861 500 µg $325 P-Cadherin None Polyclonal Goat IgG AF761 100 µg $355 Mouse

None 106020 Rat IgG2A MAB761 500 µg $325 None Polyclonal Goat IgG AF938 100 µg $355 VE-Cadherin Human

None 123413 Mouse IgG2B MAB9381 100 µg $240 None Polyclonal Goat IgG AF3320 100 µg $355 Calbindin D Human

None 401025 Mouse IgG2B MAB3320 100 µg $240 Calbindin D in Rat Hypothalamus. Calbindin D was detected in a frozen rat hypothalamus section using anti-human calbindin D monoclonal antibody (Cata- log # MAB3320). Tissue was stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxy- lin (blue).

30 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Calretinin Human/Mouse/Rat None Polyclonal Goat IgG AF5065 100 µg $355

Calretinin/Calbindin-2 in Human Brain Cortex. Calretinin was detected in paraffin-embedded hu- man brain cortex sections using anti-human/mouse/ rat Calretinin affinity-purified polyclonal antibody (Catalog # AF5065). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Calsyntenin-3 Human None Polyclonal Sheep IgG AF5244 100 µg $355

CaM Kinase IIa Human None 525501 Mouse IgG2B MAB5584 100 µg $240 CaM Kinase IId aa 402-478 Human None Polyclonal Sheep IgG AF4176 100 µg $355

CaMKIIδ in Human Cerebral Cortex. Calcium/ Calmodulin-dependent protein kinase type II delta (CaMKIId) was detected in paraffin-embedded hu- man cerebral cortex tissue sections using anti-human CaMKIId affinity-purified polyclonal antibody (Cata- log # AF4176). Tissues were stained using anti- sheep HRP-DAB detection (brown) and counter- stained with hematoxylin (blue).

cAMP Multi-species None 250532 Mouse IgG1 MAB2146 500 µg $325

Cannabinoid R2/CB2/CNR2 Human None 352114 Mouse IgG2A MAB3655 100 µg $240

CAR/NR1I3 Human None N4111 Mouse IgG1 PP-N4111-00 100 µg $380 Carbonic Anhydrase II Human None Polyclonal Sheep IgG AF2184 100 µg $355 None Polyclonal Goat IgG AF2186 100 µg $355 Human

Carbonic Anhydrase IV None 310413 Mouse IgG2B MAB2186 100 µg $240 Mouse None Polyclonal Goat IgG AF2414 100 µg $355 Human None Polyclonal Goat IgG AF2188 100 µg $355 Carbonic Anhydrase IX Mouse None Polyclonal Goat IgG AF2344 100 µg $355 None Polyclonal Goat IgG AF2489 100 µg $355 Carnosine Dipeptidase 1/CNDP1 Human

None 352216 Mouse IgG1 MAB2489 100 µg $240 None Polyclonal Goat IgG AF163 100 µg $355 CART Human Biotin Polyclonal Goat IgG BAF163 50 µg $380

CART aa 28-116 Human None 113612 Mouse IgG2B MAB163 500 µg $325 Active Caspase-3 aa 163-175 Human/Mouse None Polyclonal Rabbit IgG AF835 50 µg $285 Cleaved Caspase-3 (Asp175) Human/Mouse None 269518 Rabbit IgG MAB835 50 µg $270

Caspase-9 aa 1-134 Human None LAP6 Mouse IgG1 MAB8301 100 µg $240 Caspase-9 aa 139-330 Human None Polyclonal Goat IgG AF8301 100 µg $355 Caspr2 Human None Polyclonal Sheep IgG AF5145 100 µg $355

Caspr2 in Mouse Neurons. Caspr2 was detected in a frozen section of adult mouse brain using anti-human Caspr2 affinity-purified polyclonal anti- body (Catalog # AF5145). Cells were stained using NorthernLights-557 donkey anti-sheep IgG second- ary antibody (Catalog # NL010; red) and counter- stained with DAPI (blue).

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

None 196618 Mouse IgG3 MAB13291-050 50 µg $150 Human

b-Catenin None 196618 Mouse IgG3 MAB13291-100 100 µg $240 Human/Mouse/Rat None Polyclonal Goat IgG AF1329 100 µg $355

b-Catenin in Human Kidney Cancer. b-Catenin was detected in paraffin-embedded human kidney can- cer sections using anti-human/mouse/rat b-Catenin affinity-purified polyclonal antibody (Catalog # AF1329). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

None Polyclonal Goat IgG AF1049 100 µg $355 Human Cathepsin A/Lysosomal Carboxypeptidase A Biotin Polyclonal Goat IgG BAF1049 50 µg $380

Mouse None 191424 Rat IgG2B MAB11231 500 µg $325 None Polyclonal Goat IgG AF953 100 µg $355 Human Biotin Polyclonal Goat IgG BAF953 50 µg $380 Cathepsin B None Polyclonal Goat IgG AF965 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF965 50 µg $380 Human None Polyclonal Goat IgG AF1071 100 µg $355 Cathepsin C/DPPI None Polyclonal Goat IgG AF1034 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1034 50 µg $380 None Polyclonal Goat IgG AF1014 100 µg $355 Human Biotin Polyclonal Goat IgG BAF1014 50 µg $380 Cathepsin D None Polyclonal Goat IgG AF1029 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1029 50 µg $380 None Polyclonal Goat IgG AF1294 100 µg $355 Human Biotin Polyclonal Goat IgG BAF1294 50 µg $380 Cathepsin E None Polyclonal Goat IgG AF1130 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1130 50 µg $380 None Polyclonal Goat IgG AF2075 100 µg $355 Human

Cathepsin F None 237208 Mouse IgG1 MAB2075 500 µg $325 Mouse None Polyclonal Goat IgG AF2544 100 µg $355

Cathepsin F in Human Heart. Cathepsin F was de- tected in paraffin-embedded human heart tissue sec- tions using anti-human Cathepsin F monoclonal anti- body (Catalog # MAB2075). Tissues were stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Cathepsin H Mouse None Polyclonal Goat IgG AF1013 100 µg $355

32 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF952 100 µg $355 Human

None 204106 Rat IgG2B MAB952 500 µg $325 Cathepsin L

Human/Mouse None 204101 Rat IgG2A MAB9521 500 µg $325 Mouse None Polyclonal Goat IgG AF1515 100 µg $355

Cathepsin L in Mouse Ovary. Cathepsin L was detected in frozen sections of mouse ovary using anti-mouse Cathepsin L affinity-purified polyclonal antibody (Catalog # AF1515). Tissue was stained us- ing anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Cathepsin O Human None Polyclonal Goat IgG AF1174 100 µg $355 None Polyclonal Goat IgG AF1183 100 µg $355 Cathepsin S Human Biotin Polyclonal Goat IgG BAF1183 50 µg $380 None Polyclonal Goat IgG AF1080 100 µg $355 Cathepsin V Human

None 182016 Mouse IgG1 MAB1080 500 µg $325 None Polyclonal Goat IgG AF934 100 µg $355 Human Biotin Polyclonal Goat IgG BAF934 50 µg $380 Cathepsin X/Z/P None Polyclonal Goat IgG AF1033 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1033 50 µg $380

CBP Human/Mouse/Rat None 257003 Mouse IgG2B MAB2676 100 µg $240 Ccd1/DIXDC1 Mouse None Polyclonal Goat IgG AF5599 100 µg $355

CCK4 aa 31-199 Human/Rat None 525222 Mouse IgG1 MAB4499 100 µg $240 CCK4 in Human Colon Cancer. Colon carcinoma kinase 4 (CCK4) was detected in a paraffin-emedded section of human colon cancer using anti-human CCK4 monoclonal antibody (Catalog # MAB4499). The tissue was stained using the anti-mouse HRP- DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

CCK-A R aa 256-267 Human/Mouse/Rat None Polyclonal Rabbit IgG AF2680 50 µg $285 None Polyclonal Goat IgG AF1774 100 µg $355 Canine Biotin Polyclonal Goat IgG BAF1774 50 µg $380 CCL2/JE/MCP-1

None 280702 Mouse IgG1 MAB28171 500 µg $325

Human None 23002 Mouse IgG2B MAB2791 500 µg $325 Biotin Polyclonal Goat IgG BAF270 50 µg $380 Human

CCL3/MIP-1a None 93321 Mouse IgG1 MAB270 500 µg $325 Mouse None Polyclonal Goat IgG AF-450-NA 100 µg $355 None Polyclonal Goat IgG AF-271-NA 100 µg $355 Human Biotin Polyclonal Goat IgG BAF271 50 µg $380 CCL4/MIP-1b None Polyclonal Goat IgG AF-451-NA 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF451 50 µg $380 Human None Polyclonal Goat IgG AF-278-NA 100 µg $355 CCL5/RANTES None Polyclonal Goat IgG AF478 100 µg $355 Mouse

None 53405 Rat IgG2A MAB478 500 µg $325

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF-320-NA 100 µg $355 Human

None 43911 Mouse IgG1 MAB320 500 µg $325 None Polyclonal Goat IgG AF-420-NA 100 µg $355 CCL11/Eotaxin Biotin Polyclonal Goat IgG BAF420 50 µg $380 Mouse

Biotin 42285 Rat IgG2A BAM420 250 µg $380

None 42285 Rat IgG2A MAB420 500 µg $325 None Polyclonal Goat IgG AF802 100 µg $355 CCL16/HCC-4 Human

None 70218 Mouse IgG1 MAB328 500 µg $325 CCL17/TARC Human None Polyclonal Goat IgG AF364 100 µg $355

Human None 54909 Mouse IgG2B MAB361 500 µg $325 CCL19/MIP-3b Mouse None Polyclonal Goat IgG AF880 100 µg $355 None Polyclonal Goat IgG AF360 100 µg $355 CCL20/MIP-3a Human Biotin Polyclonal Goat IgG BAF360 50 µg $380 CCL21/6Ckine Human None Polyclonal Goat IgG AF366 100 µg $355 CCL24/Eotaxin-2/MPIF-2 Human None Polyclonal Goat IgG AF343 100 µg $355 CCL25/TECK Mouse None Polyclonal Goat IgG AF-481-NA 100 µg $355

CCL25/TECK Mouse None 89827 Rat IgG2A MAB4811 500 µg $325 CCL26/Eotaxin-3 Human None Polyclonal Goat IgG AF653 100 µg $355 None Polyclonal Goat IgG AF376 100 µg $355

Human None 124308 Mouse IgG1 MAB376 500 µg $325

None 124302 Mouse IgG2A MAB3761 500 µg $325 CCL27/CTACK None Polyclonal Goat IgG AF725 100 µg $355

Mouse Biotin 68706 Rat IgG2A BAM7251 250 µg $380

None 68706 Rat IgG2A MAB7251 500 µg $325 CCL27/CTACK in Normal Human Skin. CCL27/ CTACK was detected in a frozen tissue section of nor- mal human skin using anti-human CCL27/CTACK af- finity-purified polyclonal antibody (Catalog # AF376). Tissues were stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue).

CCR1 Human None 53504 Mouse IgG2B MAB145 500 µg $325

CCR3 Human None 61828 Rat IgG2A MAB155 500 µg $325

None 45523 Mouse IgG2B MAB181 500 µg $325 CCR5 Human

None 45549 Mouse IgG2B MAB183 500 µg $325

Human None 53103 Mouse IgG2B MAB195 500 µg $325 CCR6

Mouse None 140706 Rat IgG2A MAB590 500 µg $325

Human None 150503 Mouse IgG2A MAB197 500 µg $325 CCR7

Mouse None 4B12 Rat IgG2A MAB3477 100 µg $240 CCR7 in Human Peripheral Blood Mononuclear Cells. CCR7 was detected in human PBMCs using anti-human CCR7 monoclonal antibody (Catalog # MAB197; red). Cells were counterstained green.

None 112509 Mouse IgG2A MAB179 500 µg $325 CCR9 Human

None 248621 Mouse IgG2A MAB1791 500 µg $325

34 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

CD2 Human None 299812 Mouse IgG1 MAB18561 100 µg $240

Biotin 17A2 Rat IgG2B BAM4841 250 µg $380 CD3 Mouse

None 17A2 Rat IgG2B MAB4841 500 µg $325 CD3-expressing Cells in Mouse Brain (perivascu- lar cuffing). Cells expressing CD3 were detected in inflamed mouse brain using anti-mouse CD3 affinity- purified (Catalog # MAB4841; green). The nuclei were counterstained with DAPI (blue).

CD3e Human None UCHT1 Mouse IgG1 MAB100 500 µg $325 CD3ε in Human Peripheral Blood Mononuclear Cells (PBMCs). CD3ε was detected in human PBMCs using anti-human CD3ε monoclonal antibody (Catalog # MAB100). Cells were stained using NorthernLights-557 anti-mouse IgG secondary anti- body (Catalog # NL007; red) and counterstained with DAPI (blue).

None Polyclonal Goat IgG AF2410 100 µg $355 Canine Biotin Polyclonal Goat IgG BAF2410 50 µg $380

None 296712 Mouse IgG2B MAB2410 100 µg $240 None Polyclonal Goat IgG AF2597 100 µg $355 Feline

CD4 None 372711 Mouse IgG2A MAB25971 100 µg $240 None Polyclonal Goat IgG AF-379-NA 100 µg $355 Human

None 34930 Mouse IgG1 MAB379 500 µg $325

Biotin GK1.5 Rat IgG2B BAM554 250 µg $380 Mouse

None GK1.5 Rat IgG2B MAB554 500 µg $325 Human None Polyclonal Goat IgG AF1636 100 µg $355

CD5 Biotin 53-7.3 Rat IgG2A BAM115 250 µg $380 Mouse

None 53-7.3 Rat IgG2A MAB115 500 µg $325 Feline None Polyclonal Goat IgG AF2598 100 µg $355

CD8a Biotin 53-6.7 Rat IgG2A BAM116 250 µg $380 Mouse

None 53-6.7 Rat IgG2A MAB116 500 µg $325

CD9 Mouse None 479608 Rat IgG2A MAB5218 100 µg $240 CD14 Human Biotin Polyclonal Sheep IgG BAF383 50 µg $380 CD23/Fce RII Human None Polyclonal Goat IgG AF123 100 µg $355

CD24 Human None 528807 Mouse IgG2B MAB5248 100 µg $240 CD27/TNFRSF7 Human Biotin Polyclonal Goat IgG BAF382 50 µg $380

CD27 Ligand/TNFSF7 Human None 301731 Mouse IgG2B MAB2738 100 µg $240 CD28 Human None Polyclonal Goat IgG AF-342-PB 200 µg $355 CD30/TNFRSF8 Mouse None Polyclonal Goat IgG AF852 100 µg $355 None Polyclonal Sheep IgG AF806 100 µg $355 CD31/PECAM-1 Human

None 9G11 Mouse IgG1 BBA7 100 µg $230 CD34 Rat None Polyclonal Goat IgG AF4117 100 µg $355

CD38 Human None 240742 Mouse IgG2A MAB2404 500 µg $325

Human None 82111 Mouse IgG2B MAB6321 500 µg $325 CD40/TNFRSF5 Mouse None Polyclonal Goat IgG AF440 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF617 100 µg $355 Human

None 40804 Mouse IgG2B MAB617 500 µg $325 CD40 Ligand/TNFSF5 None Polyclonal Goat IgG AF1163 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1163 50 µg $380

CD43 Human None 290111 Mouse IgG2B MAB2038 100 µg $240

CD44 H Human None 2C5 Mouse IgG2A BBA10 200 µg $315

CD44 v3 Human None 3G5 Mouse IgG2B BBA11 200 µg $315

CD44 v4/5 Human None 3D2 Mouse IgG1 BBA25 200 µg $315

CD44 v6 Human None 2F10 Mouse IgG1 BBA13 200 µg $315

Biotin 2D1 Mouse IgG1 BAM1430 100 µg $295 Human

None 2D1 Mouse IgG1 MAB1430 100 µg $240 CD45 None Polyclonal Goat IgG AF114 100 µg $355

Mouse Biotin 30-F11 Rat IgG2B BAM114 250 µg $380

None 30-F11 Rat IgG2B MAB114 500 µg $325 CD46 Human None Polyclonal Goat IgG AF2005 100 µg $355

CD46 in Human Skin. CD46 was detected in paraf- fin-embedded human skin tissue sections using anti- human CD46 affinity-purified polyclonal antibody (Catalog # AF2005). Tissues were stained using anti- goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

CD47 Human None Polyclonal Sheep IgG AF4670 100 µg $355 None Polyclonal Goat IgG AF1866 100 µg $355 CD47 N-terminal IgV-like Extracellular Domain Mouse Biotin Polyclonal Goat IgG BAF1866 50 µg $380

Human None 394620 Mouse IgG2B MAB36441 100 µg $240 CD48/SLAMF2 Mouse None Polyclonal Goat IgG AF3327 100 µg $355 None Polyclonal Goat IgG AF2009 100 µg $355 CD55/DAF Human Biotin Polyclonal Goat IgG BAF2009 50 µg $380 CD58/LFA-3 Human None Polyclonal Goat IgG AF1689 100 µg $355 None Polyclonal Goat IgG AF1987 100 µg $355 CD59 Human Biotin Polyclonal Goat IgG BAF1987 50 µg $380

CD63 Mouse None 446703 Rat IgG1 MAB5417 100 µg $240

CD68/SR-D1 Human None 298807 Mouse IgG2B MAB20401 100 µg $240 None Polyclonal Goat IgG AF2359 100 µg $355

Human None 298633 Mouse IgG2A MAB2359 100 µg $240 CD69

None 298614 Mouse IgG2A MAB23591 100 µg $240

Mouse None 310116 Rat IgG1 MAB23861 100 µg $240 None Polyclonal Goat IgG AF1279 100 µg $355 CD72 Mouse Biotin Polyclonal Goat IgG BAF1279 50 µg $380

Human None HB15e Mouse IgG1 MAB1774 500 µg $325 CD83 Mouse None Polyclonal Goat IgG AF1437 100 µg $355

36 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE CD109 Human None Polyclonal Sheep IgG AF4385 100 µg $355

CD109 in Human Squamous Cell Carcinoma. CD109 was detected in paraffin-embedded human squamous cell carcinoma sections using anti-human CD109 affinity-purified polyclonal antibody (Catalog # AF4385). Tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxy- lin (blue).

CD151 Mouse None 455807 Rat IgG2A MAB4609 100 µg $240 None Polyclonal Goat IgG AF2530 100 µg $355 Biotin Polyclonal Goat IgG BAF2530 50 µg $380 CD155/PVR Human

None 300903 Mouse IgG1 MAB2530 100 µg $240

None 300907 Mouse IgG1 MAB25301 100 µg $240 Human None Polyclonal Goat IgG AF2724 100 µg $355 CD200 Mouse None Polyclonal Goat IgG AF3355 100 µg $355

Human None 380529 Mouse IgG2B MAB34141 100 µg $240 CD200 R1 None Polyclonal Goat IgG AF2554 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF2554 50 µg $380 Phospho-CDC2 (Y15) Human/Mouse/Rat None Polyclonal Rabbit IgG AF888 100 µg $285

CDC25A Human/Mouse/Rat None 336445 Mouse IgG2B MAB1648 100 µg $240 CDC25B aa 377-566 Human/Mouse/Rat None Polyclonal Goat IgG AF1649 100 µg $355 Human None Polyclonal Goat IgG AF2666 100 µg $355 CDCP1 Mouse None Polyclonal Sheep IgG AF4515 100 µg $355 CDNF Human None Polyclonal Goat IgG AF5097 100 µg $355

CDNF in Human Cortex. Conserved Dopamine Neu- rotrophic Factor (CDNF) was detected in paraffin- embedded human cortex sections using anti-human CDNF affinity-purified polyclonal antibody (Catalog # AF5097). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Human None Polyclonal Sheep IgG AF4384 100 µg $355 CDO Mouse None Polyclonal Goat IgG AF2429 100 µg $355

CDO Expression in Embryonic Mouse Retina. CAM- related/Down-regulated by Oncogenes (CDO) was detected in a cryostat tissue section of embryonic mouse retina (13 d.p.c.) using anti-mouse CDO poly- clonal antibody (Catalog # AF2429) and anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). Tissues were counterstained­ with hematoxylin (blue).

CDX2 Human None Polyclonal Goat IgG AF3665 100 µg $355 None Polyclonal Goat IgG AF2807 100 µg $355 CDX4 Human

None 322007 Mouse IgG2B MAB2807 100 µg $240 None Polyclonal Goat IgG AF2244 100 µg $355 CEACAM-1/CD66a Human Biotin Polyclonal Goat IgG BAF2244 50 µg $380

None 283324 Mouse IgG1 MAB22441 500 µg $325

US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com 37 R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Sheep IgG AF4128 100 µg $355 CEACAM-5/CD66e Human

None 487609 Mouse IgG2A MAB41281 100 µg $240

Cerberus 1 Human None 162836 Mouse IgG2B MAB1075 500 µg $325

CFTR C-Terminus Human None 24-1 Mouse IgG2A MAB25031 50 µg $215

CFTR R Domain Human None 13-1 Mouse IgG1 MAB1660 50 µg $215

Chitinase 3-like 1 Human None 321806 Rat IgG2B MAB2599 100 µg $240

Chk2 aa 1-234 Mouse None 235303 Rat IgG2A MAB22851 100 µg $240 Phospho-Chk2 (T68) Human None Polyclonal Rabbit IgG AF1626 100 µg $285

Human None 316223 Rat IgG1 MAB2126 100 µg $240 CHL-1/L1CAM-2 Mouse None Polyclonal Goat IgG AF2147 100 µg $355 Choline Acetyltransferase/ChAT Isoform R Human None Polyclonal Goat IgG AF3447 100 µg $355

Chondrolectin Human None 396305 Mouse IgG2B MAB2576 100 µg $240 Chordin Mouse None Polyclonal Goat IgG AF758 100 µg $355 None Polyclonal Goat IgG AF2448 100 µg $355 Human Chordin-Like 2/CHRDL2 Biotin Polyclonal Goat IgG BAF2448 50 µg $380

Mouse None 328905 Rat IgG2A MAB2520 100 µg $240

Chorionic Gonadotropin, a Chain (a HCG) Human None 381012 Mouse IgG2A MAB4169 100 µg $240 CIS-1 Human None Polyclonal Goat IgG AF3194 100 µg $355 Claspin Human None Polyclonal Goat IgG AF3310 100 µg $355

Claudin-3 Human None 385021 Mouse IgG2A MAB4620 100 µg $240

Claudin-12 Human None 393314 Mouse IgG2A MAB4284 100 µg $240 Claudin-12 in Murine NS0 Cells. Claudin-12 was detected in a Claudin-12-transfected murine NS0 my- eloma cell line using anti-human Claudin-12 mono- clonal antibody (Catalog # MAB4284). Cells were stained using NorthernLights-557 donkey anti- mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

CL-P1/COLEC12 Human None Polyclonal Goat IgG AF2690 100 µg $355 Clusterin Mouse None Polyclonal Goat IgG AF2747 100 µg $355 Clusterin Isoform 1 Human None Polyclonal Goat IgG AF2937 100 µg $355 CNP Human None Polyclonal Goat IgG AF3127 100 µg $355

CNP Propeptide Human None 347625 Rat IgG2A MAB3739 100 µg $240 None Polyclonal Total Goat IgG AB-557-NA 1 mg $355 CNTF Rat None Polyclonal Goat IgG AF-557-NA 100 µg $355 Biotin Polyclonal Goat IgG BAF557 50 µg $380 None Polyclonal Goat IgG AF-559-NA 100 µg $355 CNTF Ra Rat Biotin Polyclonal Goat IgG BAF559 50 µg $380

CNTF Ra in Rat Brain. Ciliary Neurotrophic Factor Receptor a (CNTF Ra) was detected in a cryostat tissue section of rat brain using biotinylated anti-rat CNTF Ra polyclonal antibody (Catalog # BAF559). Tissues were stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue). Antigen retrieval using Antigen retrieval-Basic Re- agent is recommended (Catalog # CTS013).

Coagulation Factor III/Tissue Factor Human None Polyclonal Goat IgG AF2339 100 µg $355

38 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF2338 100 µg $355 Coagulation Factor VII Human Biotin Polyclonal Goat IgG BAF2338 50 µg $380

None 321621 Mouse IgG1 MAB2338 100 µg $240 Coagulation Factor VII in Human PBMCs. Coagula- tion Factor VII was detected in PHA-stimulated human peripheral blood mononuclear cells (PBMCs) using anti-human coagulation factor VII monoclonal antibody (Catalog # MAB2338; red). Cells were coun- terstained (green).

Collagen II Mouse None Polyclonal Sheep IgG AF3615 100 µg $355 Collectrin Mouse None Polyclonal Sheep IgG AF4965 100 µg $355 Complement Component C1qC aa 115-245 Human None Polyclonal Goat IgG AF3337 100 µg $355 Complement Component C3d Mouse None Polyclonal Goat IgG AF2655 100 µg $355 Contactin-1 Human None Polyclonal Goat IgG AF904 100 µg $355 None Polyclonal Goat IgG AF1714 100 µg $355 Contactin-2/TAG1 Human

None 372944 Mouse IgG2B MAB1714 100 µg $240 Corneodesmosin Human None Polyclonal Sheep IgG AF5725 100 µg $355

None H8132 Mouse IgG2A PP-H8132-00 100 µg $380 COUP-TF I/NR2F1 Human

None H8132 Mouse IgG2A PP-H8132-10 100 µg $380

None H7147 Mouse IgG2A PP-H7147-00 100 µg $380 COUP-TF II/NR2F2 Human

None H7147 Mouse IgG2A PP-H7147-10 100 µg $380 COX-2 aa 18-604 Human/Mouse None Polyclonal Goat IgG AF4198 100 µg $355 None Polyclonal Sheep IgG AF1707 100 µg $355 Human Biotin Polyclonal Sheep IgG BAF1707 50 µg $380 C-Reactive Protein/CRP Mouse None Polyclonal Goat IgG AF1829 100 µg $355 Rat None Polyclonal Goat IgG AF1744 100 µg $355

C-Reactive Protein in Human Kidney Cancer. C- Reactive Protein (CRP) was detected in paraffin- embedded human kidney cancer tissue sections us- ing anti-human CRP affinity-purified polyclonal anti- body (Catalog # AF1707). Tissues were stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

None Polyclonal Goat IgG AF2989 100 µg $355 CREB aa 136-341 Human

None 321120 Mouse IgG1 MAB2989 100 µg $240 CRELD1 Mouse None Polyclonal Goat IgG AF4116 100 µg $355 Human None Polyclonal Goat IgG AF2796 100 µg $355 CRHBP Mouse None Polyclonal Goat IgG AF3087 100 µg $355

CRHR-1 Human None 343919 Mouse IgG2A MAB3930 100 µg $240 None Polyclonal Sheep IgG AF1538 100 µg $355 Cripto Mouse Biotin Polyclonal Sheep IgG BAF1538 50 µg $380

None 237603 Rat IgG2B MAB1538 150 µg $240

Cripto 1 Human None 89646 Mouse IgG1 MAB2771 100 µg $240

CRISP-3 Human None 295203 Mouse IgG1 MAB23971 100 µg $240 CRY1 Human/Mouse None Polyclonal Goat IgG AF3764 100 µg $355

Cryptic Mouse None 221428 Rat IgG2B MAB18401 100 µg $240 CSL Human None Polyclonal Goat IgG AF4079 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF660 100 µg $355 CTGF/CCN2 C-Terminus Human

None 88430 Mouse IgG1 MAB660 500 µg $325 CTLA-4 Human None Polyclonal Goat IgG AF-386-PB 100 µg $355 Cubilin Human None Polyclonal Sheep IgG AF3700 100 µg $355 None Polyclonal Goat IgG AF365 100 µg $355 Human

CX3CL1/Fractalkine Chemokine Domain None 81513 Mouse IgG1 MAB3651 500 µg $325 Rat None Polyclonal Goat IgG AF537 100 µg $355 CXADR Mouse None Polyclonal Goat IgG AF2654 100 µg $355 CXCL1/GROa/KC/CINC-1 Rat None Polyclonal Goat IgG AF-515-NA 100 µg $355 None Polyclonal Goat IgG AF-452-NA 100 µg $355 CXCL2/GROb/MIP-2/CINC-3 Mouse Biotin Polyclonal Goat IgG BAF452 50 µg $380 None Polyclonal Goat IgG AF254 100 µg $355 CXCL5/ENA-78 Human

None 33160 Mouse IgG1 MAB254 500 µg $325 None Polyclonal Goat IgG AF793 100 µg $355 Mouse CXCL7/Thymus Chemokine-1 Biotin Polyclonal Goat IgG BAF793 50 µg $380 Rat None Polyclonal Goat IgG AF1116 100 µg $355 None Polyclonal Goat IgG AF1608 100 µg $355 Canine Biotin Polyclonal Goat IgG BAF1608 50 µg $380

None 258911 Mouse IgG1 MAB1608 100 µg $240 CXCL8/IL-8 Feline None Polyclonal Goat IgG AF2277 100 µg $355 None Polyclonal Goat IgG AF-208-NA 100 µg $355 Human Biotin Polyclonal Goat IgG BAF208 50 µg $380

None 6217 Mouse IgG1 MAB208 500 µg $325

CXCL8/IL-8 NorthernLights-conjugated Human NL637 6217 Mouse IgG1 NL208V 500 µL $345 None Polyclonal Goat IgG AF392 100 µg $355 CXCL9/MIG Human

None 49106 Mouse IgG1 MAB392 500 µg $325 Human None Polyclonal Goat IgG AF-266-NA 100 µg $355 CXCL10/IP-10/CRG-2 None Polyclonal Goat IgG AF-466-NA 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF466 50 µg $380

CXCL12/SDF-1 Human/Mouse None 79018 Mouse IgG1 MAB350 500 µg $325 Human None Polyclonal Goat IgG AF801 100 µg $355 CXCL13/BLC/BCA-1 Mouse None Polyclonal Goat IgG AF470 100 µg $355 None Polyclonal Goat IgG AF976 100 µg $355 CXCL16 Human Biotin Polyclonal Goat IgG BAF976 50 µg $380

CXCR4 Feline None 374606 Mouse IgG2A MAB4287 100 µg $240

Human None 44716 Mouse IgG2B MAB172 500 µg $325 CXCR4

Mouse None 247506 Rat IgG2B MAB21651 100 µg $325

CXCR5 Human None 51505 Mouse IgG2B MAB190 500 µg $325

CXCR6 Human None 56811 Mouse IgG2B MAB699 500 µg $325

None 358426 Mouse IgG2A MAB4227 100 µg $240 CXCR7/RDC-1 Human

None 11G8 Mouse IgG1 MAB42273 100 µg $240

Cyclin D1 Human None 328303 Mouse IgG1 MAB4314 100 µg $240

40 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Sheep IgG AF4055 100 µg $355 Cyr61/CCN1 Mouse

None 466305 Rat IgG2B MAB4864 100 µg $240 Cyr61 in Developing Embryonic Mouse Rib. Cyr61 was detected in cryostat sections of E13 mouse em- bryo using anti-mouse Cry61 affinity-purified poly- clonal antibody (Catalog # AF4055). Tissues were stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counter- stained with hematoxylin (blue).

Cystatin A Human None Polyclonal Goat IgG AF1407 100 µg $355 Human None Polyclonal Goat IgG AF1408 100 µg $355 Cystatin B

Mouse None 227807 Rat IgG2A MAB14091 500 µg $325 Cystatin B in Mouse Cerebellum. Cystatin B was de- tected in a frozen section of mouse cerebellum using anti-mouse Cystatin B monoclonal antibody (Catalog # MAB14091). Tissue was subjected to antigen re- trieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).

Human None Polyclonal Goat IgG AF1196 100 µg $355 Cystatin C Mouse None Polyclonal Goat IgG AF1238 100 µg $355 None Polyclonal Goat IgG AF1286 100 µg $355 Cystatin E/M Human Biotin Polyclonal Goat IgG BAF1286 50 µg $380

None 211515 Mouse IgG2B MAB1286 500 µg $325

Cytochrome c Human/Equine None 2B5F8 Mouse IgG2A MAB898 100 µg $240

Cytokeratin 8 Human None LP3K Mouse IgG1 MAB3165 100 µg $240

Cytokeratin 14 Human None LL001 Mouse IgG2A MAB3164 100 µg $240

Cytokeratin 19 Human None BA17 Mouse IgG1 MAB3506 100 µg $240 DAN Human None Polyclonal Goat IgG AF955 100 µg $355

DANCE Human None 293904 Mouse IgG1 MAB3095 100 µg $240

DARC Human None 358307 Mouse IgG2A MAB4139 100 µg $240

DARPP-32 aa 51-204 Mouse/Rat None 375604 Rat IgG2A MAB4230 100 µg $240 None Polyclonal Goat IgG AF142 100 µg $355 DcR3/TNFRSF6B Human Biotin Polyclonal Goat IgG BAF142 50 µg $380

DC-SIGN Human None 120507 Mouse IgG2B MAB161 500 µg $325

DC-SIGN+DC-SIGNR Human None 120612 Mouse IgG2A MAB1621 500 µg $325 None Polyclonal Goat IgG AF2378 100 µg $355

DcTRAIL R1/TNFRSF23 Mouse Biotin 270012 Rat IgG2A BAM2378 100 µg $380

None 270012 Rat IgG2A MAB2378 100 µg $240 DDR1 Human None Polyclonal Goat IgG AF2396 100 µg $355 DDR2 Human None Polyclonal Goat IgG AF2538 100 µg $355

None 290814 Mouse IgG2B MAB2538 100 µg $240 DDR2 Human

None 290804 Mouse IgG2B MAB25381 100 µg $240 DEC-205/CD205 Human None Polyclonal Goat IgG AF2047 100 µg $355 None Polyclonal Goat IgG AF143 100 µg $355 Human

None 115402 Mouse IgG1 MAB143 500 µg $325 Decorin None Polyclonal Goat IgG AF1060 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1060 50 µg $380

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

Dectin-1/CLEC7A Human None 259931 Mouse IgG2B MAB1859 100 µg $240 b-Defensin 2/BD-2 Human None Polyclonal Goat IgG AF2758 100 µg $355 Desert Hedgehog C-Terminus Mouse None Polyclonal Goat IgG AF196 100 µg $355 None Polyclonal Goat IgG AF733 100 µg $355 Desert Hedgehog N-Terminus Mouse Biotin Polyclonal Goat IgG BAF733 50 µg $380 Desmin Human None Polyclonal Goat IgG AF3844 100 µg $355

Desmin in Human Heart. Desmin was detected in paraffin-embedded human heart sections using anti- human desmin affinity-purified polyclonal antibody (Catalog # AF3844). Tissue was stained using the anti- goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

None Polyclonal Goat IgG AF944 100 µg $355 Desmoglein-1 Propeptide Human Biotin Polyclonal Goat IgG BAF944 50 µg $380 None Polyclonal Goat IgG AF1720 100 µg $355 Desmoglein-3 Human

None 216519 Mouse IgG2B MAB1720 500 µg $325 Dishevelled-1 Human None Polyclonal Goat IgG AF3316 100 µg $355

Dishevelled-1 in Human Skeletal Muscle. Dishev- elled-1 was detected in a paraffin-embedded human skeletal muscle section using anti-human dishev- elled-1 affinity-purified polyclonal antibody (Catalog # AF3316). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

Dishevelled-3 Human None Polyclonal Sheep IgG AF3754 100 µg $355 None Polyclonal Goat IgG AF1765 100 µg $355

Dkk-1 Mouse Biotin 259631 Rat IgG2B BAM1765 100 µg $380

None 259631 Rat IgG2B MAB1765 100 µg $240 Dkk-2 Mouse None Polyclonal Goat IgG AF2435 100 µg $355 None Polyclonal Goat IgG AF1118 100 µg $355 Dkk-3 Human Biotin Polyclonal Goat IgG BAF1118 50 µg $380 DLEC/CLEC4C/BDCA2 Human None Polyclonal Goat IgG AF1376 100 µg $355

Human None 251123 Mouse IgG2B MAB18181 100 µg $240 DLL1 Mouse/Rat None Polyclonal Sheep IgG AF5026 100 µg $355 Rat None Polyclonal Sheep IgG AF3970 100 µg $355

DLL1 Expression in Mouse Embyronic Stomach. Delta-like 1 (DLL1) was detected in a cryostat tissue section of mouse E13.5 embryonic stomach using anti-rat DLL1 affinity-purified polyclonal antibody (Catalog # AF3970). Tissues were stained using NorthernLights-557-conjugated anti-sheep second- ary antibody (Catalog # NL010; red) and counter- stained with DAPI (blue).

DMC/VCC-1 Human None 422208 Mouse IgG2B MAB4207 100 µg $240

42 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE DMP-1 Mouse None Polyclonal Sheep IgG AF4386 100 µg $355

DMP-1 in Developing Mouse Teeth. Dentin Matrix Protein 1 (DMP-1) was detected in a cryostat section of developing embryonic (E15.5) mouse teeth using anti-mouse DMP-1 affinity-purified polyclonal anti- body (Catalog # AF4386). Cells were stained using NorthernLights-557 donkey anti-sheep IgG second- ary antibody (Catalog # NL010; red) and counter- stained with DAPI (blue).

None Polyclonal Goat IgG AF3646 100 µg $355 Human Biotin Polyclonal Goat IgG BAF3646 50 µg $380 DNER None Polyclonal Goat IgG AF2254 100 µg $355 Mouse

Biotin 298306 Rat IgG1 BAM2254 100 µg $380

Mouse None 298306 Rat IgG1 MAB2254 100 µg $240 DOK4 Human None Polyclonal Sheep IgG AF4826 100 µg $355

DOK4 in Human Kidney. Downstream of kinase 4 (DOK4) was detected in a paraffin-embedded section of human kidney using anti-human DOK4 affinity- purified polyclonal antibody (Catalog # AF4826). Tissues were stained using anti-sheep HRP-DAB detection reagents (brown) and counterstained with hematoxylin (blue).

Dopa Decarboxylase/DDC Human None Polyclonal Goat IgG AF3564 100 µg $355 Dopamine Transporter C-Terminus Human/Mouse/Primate None Polyclonal Rabbit IgG PPS068 100 µL $355 Dopamine Transporter Extracellular Loop 2 Human/Primate None Polyclonal Rabbit IgG PPS069 100 µL $355 DPP6 Human None Polyclonal Goat IgG AF2360 100 µg $355 Human None Polyclonal Sheep IgG AF3674 100 µg $355 DPPA4 Mouse None Polyclonal Goat IgG AF3730 100 µg $355 Human None Polyclonal Goat IgG AF3125 100 µg $355 DPPA5/ESG1 None Polyclonal Goat IgG AF3984 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF3984 50 µg $380 Human None Polyclonal Goat IgG AF1180 100 µg $355 DPPIV/CD26 None Polyclonal Goat IgG AF954 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF954 50 µg $380 DR3/TNFRSF25 Mouse None Polyclonal Goat IgG AF2437 100 µg $355 None Polyclonal Goat IgG AF3666 100 µg $355 DSCAM Long Isoform Human

None 399212 Mouse IgG2B MAB36661 100 µg $240 DSCAM in Human A172 Cells. Down Syndrome Cell Adhesion Molecule (DSCAM) was detected in human A172 glioblastoma cell line using anti-human DSCAM affinity-purified polyclonal antibody (Catalog # AF3666). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

DSPG3 Human None 329707 Mouse IgG1 MAB3110 100 µg $240

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

Human None 96201 Mouse IgG1 MAB859 500 µg $325 None Polyclonal Goat IgG AF759 100 µg $355 Dtk

Mouse Biotin 109646 Rat IgG1 BAM759 100 µg $380

None 109646 Rat IgG1 MAB759 500 µg $325 Human/Mouse/Rat/Bovine/ None Polyclonal Sheep IgG PPS004 100 µL $355 Phospho-Dynamin (S774) Canine/Chicken/Primate DYRK1A aa 438-571 Human/Rat None Polyclonal Sheep IgG AF5407 100 µg $355 E2F-1 Human None Polyclonal Sheep IgG AF4825 100 µg $355

EAR2/NR2F6 Human None N2025 Mouse IgG2A PP-N2025-00 100 µg $380 ECE-1 Human None Polyclonal Goat IgG AF1784 100 µg $355

ECE-2 Human None 233914 Rat IgG2A MAB16451 100 µg $240 ECM-1 Human None Polyclonal Sheep IgG AF3937 100 µg $355

ECM-1 in Human Colon Cancer. Extracellular matrix protein-1 (ECM-1) was detected in paraffin-embed- ded sections of human colon cancer using anti- human ECM-1 affinity-purified polyclonal antibody (Catalog # AF3937). Tissue was stained using anti- sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxy- lin (blue).

None Polyclonal Goat IgG AF236 100 µg $355

Human None 10825 Mouse IgG1 MAB236 500 µg $325

EGF None 10827 Mouse IgG1 MAB636 500 µg $325 None Polyclonal Goat IgG AF2028 100 µg $355 Mouse

None 463004 Rat IgG2A MAB5256 100 µg $240 None Polyclonal Goat IgG AF1280 100 µg $355 EGF R/ErbB1 Mouse Biotin Polyclonal Goat IgG BAF1280 50 µg $380 Phospho-EGF R/ErbB1 (Y1173) Human None Polyclonal Rabbit IgG AF1095 50 µg $285 Phospho-EGF R/ErbB1 (Y845) Human None Polyclonal Rabbit IgG AF3394 50 µg $285 None Polyclonal Goat IgG AF2818 100 µg $355 EGR1 Human

None 406913 Rat IgG2A MAB2818 100 µg $240 EGR1 in Human Prostate Cancer. Early growth re- sponse 1 (EGR1) was detected in a paraffin-embed- ded human prostate cancer tissue section using anti- human EGR1 monoclonal antibody (Catalog # MAB2818). Tissues were stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxy- lin (blue).

eIF4E Human/Mouse/Rat None 299910 Mouse IgG2B MAB3228 100 µg $240 ELF3 aa 375-552 Human/Mouse None Polyclonal Goat IgG AF5787 100 µg $355 EMAP-II Human None Polyclonal Goat IgG AF1910 100 µg $355

EMAP-II in Human Colon Cancer. Endothelial Monocyte-activation Polypeptide II (EMAP-II) was de- tected in a paraffin-embedded human colon cancer section using anti-human EMAP-II affinity-purified polyclonal antibody (Catalog # AF1910). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counter- stained with hematoxylin (blue).

44 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE EMMPRIN Human None Polyclonal Goat IgG AF972 100 µg $355

EMR1 Mouse None 521204 Rat IgG2A MAB5580 100 µg $240 None Polyclonal Goat IgG AF1097 100 µg $355 Human Biotin Polyclonal Goat IgG BAF1097 50 µg $380 Endoglin/CD105 None Polyclonal Goat IgG AF1320 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1320 50 µg $380

None 209701 Rat IgG2A MAB1320 100 µg $240 None Polyclonal Goat IgG AF1524 100 µg $355 Human

Endoglycan/PODLX2 None 211816 Mouse IgG2A MAB1524 100 µg $240 Mouse None Polyclonal Goat IgG AF3534 100 µg $355

Endoglycan in Mouse Testis. Endoglycan was de- tected in a cryostat section of mouse testis using anti- mouse Endoglycan affinity-purified polyclonal anti- body (Catalog # AF3534). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Endomucin Mouse None Polyclonal Goat IgG AF4666 100 µg $355 Endorepellin/Perlecan Human None Polyclonal Goat IgG AF2364 100 µg $355 None Polyclonal Goat IgG AF1098 100 µg $355 Human Endostatin Biotin Polyclonal Goat IgG BAF1098 50 µg $380 Mouse None Polyclonal Goat IgG AF570 100 µg $355 EDNRB/Endothelin R Type B Human None Polyclonal Sheep IgG AF4496 100 µg $355

Endothelin-1 C-Terminus, None 3G10 Rat IgG2B MAB3440 100 µg $240 aa 53-73 (EDN1) and 15-22 (Mature) Human

Engrailed-2 Human None 279122 Mouse IgG2B MAB2600 100 µg $240 Enolase 2 Human/Mouse None Polyclonal Sheep IgG AF5169 100 µg $355 ENPP-7/Alk-SMase Mouse None Polyclonal Sheep IgG AF5160 100 µg $355 None Polyclonal Goat IgG AF1052 100 µg $355 EN-RAGE/S100A12 Human Biotin Polyclonal Goat IgG BAF1052 50 µg $380

EpCAM/TROP-1 Human None 158210 Mouse IgG2A MAB960 500 µg $325 None Polyclonal Goat IgG AF2245 100 µg $355 Human Biotin Polyclonal Goat IgG BAF2245 50 µg $380

EPCR None 304519 Mouse IgG2B MAB22451 100 µg $240 None Polyclonal Goat IgG AF2749 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF2749 50 µg $380

EPCR in Normal Human Skin. Endothelial Protein C Receptor (EPCR) was detected in a paraffin-embed- ded human skin section using anti-human EPCR monoclonal antibody (Catalog # MAB22451). The tis- sue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Human None Polyclonal Goat IgG AF638 100 µg $355 EphA1 None Polyclonal Goat IgG AF3034 100 µg $355 Mouse

None 404517 Rat IgG2A MAB30341 100 µg $240

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF3035 100 µg $355 Human

None 371805 Mouse IgG2A MAB3035 100 µg $240 EphA2 None Polyclonal Goat IgG AF639 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF639 50 µg $380 EphA3 Mouse None Polyclonal Goat IgG AF640 100 µg $355 EphA4 Mouse None Polyclonal Goat IgG AF641 100 µg $355 None Polyclonal Goat IgG AF541 100 µg $355 EphA5 Rat

None 86731 Mouse IgG1 MAB541 500 µg $325 EphA5 in Human U-118 MG Cells. EphA5 was detected in human U-118 MG glioblastoma cell line using anti-rat EphA5 monoclonal antibody (Catalog # MAB541). Cells were stained using Northern- Lights-557 donkey anti-mouse IgG secondary anti- body (Catalog # NL007; red) and counterstained with DAPI (blue).

None Polyclonal Goat IgG AF542 100 µg $355 EphB1 Rat

None 88512 Mouse IgG1 MAB5421 100 µg $240 None Polyclonal Goat IgG AF467 100 µg $355

EphB2 Mouse None 512012 Rat IgG2A MAB467 100 µg $240

None 512001 Rat IgG2A MAB4671 100 µg $240 EphB2 in Embryonic Mouse Brain. EphB2 was de- tected in a frozen section of E15 mouse brain using anti-mouse EphB2 affinity-purified polyclonal anti- body (Catalog # AF467). Tissues were stained using NorthernLights-557 secondary antibody (Catalog # NL001; red) and counterstained (green).

EphB3 Mouse None Polyclonal Goat IgG AF432 100 µg $355 Human None Polyclonal Goat IgG AF3038 100 µg $355 EphB4 None Polyclonal Goat IgG AF446 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF446 50 µg $380 Ephrin-A2 Mouse None Polyclonal Goat IgG AF603 100 µg $355

Ephrin-A2 in Rat Embryonic Neurons. Ephrin-A2 was detected in rat embryonic hippocampal neurons using anti-mouse Ephrin-A2 affinity-purified poly- clonal antibody (Catalog # AF603; green). Astrocytes were stained using an anti-GFAP antibody (red).

Ephrin-A5 Human None Polyclonal Goat IgG AF3743 100 µg $355 Ephrin-B1 Mouse None Polyclonal Goat IgG AF473 100 µg $355 None Polyclonal Goat IgG AF496 100 µg $355 Ephrin-B2 Mouse Biotin Polyclonal Goat IgG BAF496 50 µg $380 Ephrin-B3 Human None Polyclonal Goat IgG AF395 100 µg $355 Epigen Mouse None Polyclonal Goat IgG AF1127 100 µg $355

46 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF2568 100 µg $355 Epimorphin/Syntaxin 2 Mouse

None 338930 Rat IgG2A MAB2568 100 µg $240 Epimorphin/Syntaxin 2 in Mouse Eye. Epimorphin was detected in frozen embryonic mouse eye cup sections using anti-mouse Epimorphin monoclonal antibody (Catalog # MAB2568). Tissue was stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).

Epiregulin Human None Polyclonal Goat IgG AF1195 100 µg $355 None Polyclonal Goat IgG AF1129 100 µg $355 Biotin Polyclonal Goat IgG BAF1129 50 µg $380 ErbB2/Her2 Human

None 191924 Mouse IgG2B MAB1129 500 µg $325

None 416711 Mouse IgG2B MAB11291 100 µg $240 Phospho-ErbB2 in Human Breast Cancer. Phos- pho-ErbB2 was detected in paraffin-embedded hu- man breast cancer tissue sections using anti-human phospho-ErbB2 affinity-purified polyclonal antibody (Catalog # AF1768). Tissues were stained using anti- rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxy- lin (blue)

Phospho-ErbB2/Her2 (Y1196) Human None Polyclonal Rabbit IgG AF4438 100 µg $285 Phospho-ErbB2/Her2 (Y1248) Human None Polyclonal Rabbit IgG AF1768 50 µg $285

None 66219 Mouse IgG1 MAB348 500 µg $325 Human

ErbB3/Her3 None 526806 Mouse IgG2B MAB3483 100 µg $240 Mouse None Polyclonal Sheep IgG AF4518 100 µg $355

ERK1/ERK2 Human/Mouse/Rat None 216703 Mouse IgG1 MAB1576 100 µg $240 Phosphorylated ERK1/ERK2 Expression in Rat Brain Cortex. Phosphorylated ERK1/ERK2 was de- tected in cryostat tissue sections of rat brain cortex using anti-human/mouse/rat phospho-ERK1/ERK2 affinity-purified polyclonal antibody (Catalog # AF1018). Tissue was stained using the anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxy- lin (blue).

Phospho-ERK1/ERK2 None Polyclonal Rabbit IgG AF1018 100 µg $285 (ERK1 T202/Y204, ERK2 T185/Y187) Human/Mouse/Rat ERK1 Human/Mouse/Rat None Polyclonal Rabbit IgG AF1575 50 µg $285

ERK1 aa 1-28 Human None 250603 Mouse IgG2A MAB1940 100 µg $240 None Polyclonal Rabbit IgG AF1230 100 µg $285 ERK2 Human/Mouse/Rat None Polyclonal Goat IgG AF12301 100 µg $355

None 191801 Mouse IgG2A MAB1230 100 µg $240

ERK3 aa 344-721 Human None 335102 Mouse IgG2B MAB3196 100 µg $240

ERK4 aa 1-126 Human/Mouse/Rat None 355810 Rat IgG2A MAB3914 100 µg $240 ERK5/BMK1 aa 1-432 Human/Mouse None Polyclonal Goat IgG AF2848 100 µg $355

None H5844 Mouse IgG2A PP-H5844-00 100 µg $380 ERRa/NR3B1 Human

None H5844 Mouse IgG2A PP-H5844-10 100 µg $380

ERRb/NR3B2 Human None H6705 Mouse IgG2A PP-H6705-00 100 µg $380

ERRg/NR3B3 Human None H6812 Mouse IgG2A PP-H6812-00 100 µg $380

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Erythropoietin Human None Polyclonal Total Rabbit IgG AB-286-NA 1 mg $355 None Polyclonal Goat IgG AF1390 100 µg $355 Erythropoietin R Mouse Biotin Polyclonal Goat IgG BAF1390 50 µg $380 None Polyclonal Goat IgG AF2688 100 µg $355

Human None 408519 Mouse IgG2B MAB4204 100 µg $240

ESAM None 408522 Mouse IgG2B MAB42041 100 µg $240 None Polyclonal Goat IgG AF2827 100 µg $355 Mouse

None 340236 Rat IgG2A MAB28271 100 µg $240 ESAM in Human Kidney. Endothelial Cell Adhesion Molecule (ESAM) was detected in paraffin-embed- ded human kidney sections using anti-human ESAM monoclonal antibody (Catalog # MAB42041). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-mouse HRP-DAB Cell and Tissue Stain- ing Kit (Catalog # CTS002; brown). Tissue was coun- terstained with hematoxylin (blue).

ESGP Mouse None Polyclonal Sheep IgG AF4580 100 µg $355 None Polyclonal Sheep IgG AF5715 100 µg $355 ERa/NR3A1 Human

None H4624 Mouse IgG2A PP-H4624-00 100 µg $380 EZH2 Human/Mouse None Polyclonal Goat IgG AF4767 100 µg $355 None Polyclonal Goat IgG AF2964 100 µg $355 Human

None 328607 Mouse IgG2A MAB2964 100 µg $240 FABP1 None Polyclonal Goat IgG AF1565 100 µg $355 Rat

None 220119 Mouse IgG2B MAB1565 100 µg $240 Human None Polyclonal Goat IgG AF3078 100 µg $355 FABP2 Rat None Polyclonal Goat IgG AF1486 100 µg $355 None Polyclonal Sheep IgG AF1678 100 µg $355 FABP3 Human Biotin Polyclonal Sheep IgG BAF1678 50 µg $380

None 242305 Rat IgG2A MAB1678 100 µg $240 Human None Polyclonal Goat IgG AF3150 100 µg $355 None Polyclonal Goat IgG AF1443 100 µg $355 FABP4 Mouse Biotin Polyclonal Goat IgG BAF1443 50 µg $380

None 258417 Rat IgG2A MAB1443 100 µg $240 FABP4 in Mouse Mesenchymal Stem Cells. Fatty Acid Binding Protein 4 (FABP4) was detected in mouse mesenchymal stem cells using anti-mouse FABP4 affinity-purified polyclonal antibody (Catalog # AF1443). Cells were stained using anti-goat North- ernLights-557 (Catalog # NL001; red) and counter- stained with DAPI (blue).

None Polyclonal Goat IgG AF3077 100 µg $355 Human

FABP5FABP5 None 311215 Rat IgG2A MAB3077 100 µg $240 Mouse None Polyclonal Goat IgG AF1476 100 µg $355 FABP5 Mouse Biotin Polyclonal Goat IgG BAF1476 50 µg $380 None Polyclonal Goat IgG AF3880 100 µg $355 FABP6 Human Biotin Polyclonal Goat IgG BAF3880 50 µg $380 FABP7 Human None Polyclonal Goat IgG AF3166 100 µg $355 None Polyclonal Goat IgG AF2750 100 µg $355 FABP9 Mouse

None 324926 Rat IgG2A MAB2750 100 µg $240

48 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

Human None 495919 Mouse IgG2B MAB4467 100 µg $240 FAK aa 213-412 Human/Mouse/Rat None Polyclonal Sheep IgG AF4467 100 µg $355

FAK in Human Hippocampus. Focal adhesion ki- nase 1 (FAK) was detected in paraffin-embedded hu- man brain hippocampus sections using anti-human/ mouse/rat FAK affinity-purified polyclonal antibody (Catalog # AF4467). The tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Cat- alog # CTS019; brown) and counterstained with he- matoxylin (blue).

Fibroblast Activation Protein a/FAP Human None Polyclonal Sheep IgG AF3715 100 µg $355 None Polyclonal Goat IgG AF2267 100 µg $355 Feline Biotin Polyclonal Goat IgG BAF2267 50 µg $380

Fas/TNFRSF6/CD95 Human None DX2 Mouse IgG1 MAB142 500 µg $325 Mouse None Polyclonal Goat IgG AF435 100 µg $355 Rat None Polyclonal Goat IgG AF2159 100 µg $355 Human None Polyclonal Goat IgG AF126 100 µg $355 Fas Ligand/TNFSF6 None Polyclonal Goat IgG AF1858 100 µg $355 Rat

None 252029 Mouse IgG2B MAB1858 500 µg $325

FATP1 Human None 308420 Mouse IgG2B MAB3304 100 µg $240

Fcg RI/CD64 Human None 10.1 Mouse IgG1 MAB1257 500 µg $325 Fcg RII/RIII (CD32/CD16) Mouse None Polyclonal Goat IgG AF1460 100 µg $355 None Polyclonal Goat IgG AF1330 100 µg $355 Fcg RIIB/CD32b Human Biotin Polyclonal Goat IgG BAF1330 50 µg $380 Fcg RIIIA/B (CD16) Mouse None Polyclonal Goat IgG AF1960 100 µg $355 None Polyclonal Goat IgG AF2426 100 µg $355 FCRL4/FcRH4 Human Biotin Polyclonal Goat IgG BAF2426 50 µg $380 Fetuin A/AHSG Human None Polyclonal Goat IgG AF1184 100 µg $355 FGF acidic Human None Polyclonal Goat IgG AF232 100 µg $355 None Polyclonal Total Goat IgG AB-233-NA 1 mg $355 FGF basic Human None Polyclonal Goat IgG AF-233-NA 100 µg $355 Biotin Polyclonal Goat IgG BAF233 50 µg $380 None Polyclonal Goat IgG AF1206 100 µg $355 FGF-3 Human

None 254625 Mouse IgG2A MAB1206 500 µg $325 None Polyclonal Goat IgG AF235 100 µg $355 FGF-4 Human Biotin Polyclonal Goat IgG BAF235 50 µg $380 None Polyclonal Goat IgG AF-237-NA 100 µg $355 FGF-5 Human

None 221903 Mouse IgG1 MAB2371 500 µg $325

Canine None 299026 Mouse IgG1 MAB1957 100 µg $240 KGF/FGF-7 Human None Polyclonal Goat IgG AF-251-NA 250 µg $315

FGF-8 Human/Mouse None 47109 Mouse IgG1 MAB323 500 µg $325 FGF-8b Isoform Mouse None Polyclonal Goat IgG AF-423-NA 100 µg $355 FGF-9 Human None Polyclonal Goat IgG AF-273-NA 100 µg $355 FGF-10 Human None Polyclonal Goat IgG AF345 100 µg $355

FGF-17 Human None 121630 Mouse IgG2B MAB319 500 µg $325

FGF-19 Human None 117601 Mouse IgG1 MAB969 500 µg $325 None Polyclonal Goat IgG AF2547 100 µg $355 FGF-20 Human

None 272317 Rat IgG2A MAB2547 100 µg $240

FGF-21 Human None 461804 Mouse IgG2B MAB25372 100 µg $240

FGF-23 Mouse None 283507 Rat IgG2A MAB26291 100 µg $240 FGF-BP Rat None Polyclonal Goat IgG AF1413 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

FGF R2 Human None 98739 Mouse IgG1 MAB6843 500 µg $325

FGF R3 (IIIc) Human None 136312 Mouse IgG1 MAB7662 500 µg $325

Human None 240929 Rat IgG2A MAB6852 100 µg $240 FGF R4 None Polyclonal Goat IgG AF2265 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF2265 50 µg $380 FGF R5/FGFRL1 Mouse None Polyclonal Goat IgG AF1899 100 µg $355

Fgr Human None 349331 Mouse IgG2A MAB32071 100 µg $240 Fgr aa 1-82 Human/Rat None Polyclonal Goat IgG AF3207 100 µg $355 None Polyclonal Sheep IgG AF1918 100 µg $355

Fibronectin Human None P1H11 Mouse IgG1 MAB1918 100 µg $240 None P1F11 Mouse IgM MAB19181 100 µg $240

Fibronectin in Human WS-1 Cells. Fibronectin was detected in WS-1 human dermal fibroblasts us- ing anti-human Fibronectin monoclonal antibody (Catalog # MAB19181). Cells were stained using NorthernLights-557 donkey anti-mouse IgG second- ary antibody (Catalog # NL007; red) and counter- stained with DAPI (blue).

FKBP38 aa 1-326 Human/Mouse/Rat None Polyclonal Goat IgG AF3580 100 µg $355 FKBP51 Human/Mouse/Rat None Polyclonal Goat IgG AF4094 100 µg $355

FKBP51 in Human Prostate. FK506 Binding Protein, 51 kDa molecular weight (FKBP51), was detected in paraffin-embedded human prostate sections using anti-human/mouse/rat FKBP51 affinity-purified poly- clonal antibody (Catalog # AF4094). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counter- stained with hematoxylin (blue).

FKBP52 Human None 425123 Rat IgG1 MAB4095 100 µg $240 None Polyclonal Goat IgG AF1255 100 µg $355 Follistatin-related Gene Protein/FLRG Mouse Biotin Polyclonal Goat IgG BAF1255 50 µg $380

Flt-3/Flk-2 Mouse None 113308 Rat IgG2A MAB7681 100 µg $240 Phospho-Flt-3/Flk-2 (Y591) Human None Polyclonal Rabbit IgG AF368 50 µg $285 Flt-3 Ligand Feline None Polyclonal Goat IgG AF2467 100 µg $355 None Polyclonal Goat IgG AF669 100 µg $355 Follistatin Human

None 85918 Mouse IgG2A MAB669 500 µg $325 Human None Polyclonal Goat IgG AF1694 100 µg $355 Follistatin-like 1/FSL1

Mouse/Rat None 228208 Rat IgG2B MAB17381 100 µg $240

FOLR1 Human None 548908 Mouse IgG1 MAB5646 100 µg $240 FosB/G0S3 Human None Polyclonal Goat IgG AF2214 100 µg $355 None Polyclonal Sheep IgG AF4248 100 µg $355 FoxH1 Human Biotin Polyclonal Sheep IgG BAF4248 50 µg $380 None Polyclonal Goat IgG AF3619 100 µg $355 FoxJ1 Human

None 407003 Rat IgG2B MAB3619 100 µg $240

50 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE FoxM1 Human None Polyclonal Sheep IgG AF3975 100 µg $355

FoxM1 in Human U2OS Cells. FoxM1 was detected in human osteosarcoma U2OS cells using anti- human FoxM1 affinity-purified polyclonal antibody (Catalog # AF3975). Cells were stained using NorthernLights-557 donkey anti-sheep IgG second- ary antibody (Catalog # NL010; red) and counter- stained (green).

FoxP2 Human/Mouse None Polyclonal Sheep IgG AF5647 100 µg $355 FoxP3 Human None Polyclonal Goat IgG AF3240 100 µg $355

FoxP3 in Human Tonsil. FoxP3 was detected in par- affin-embedded human tonsil sections using anti- human FoxP3 affinity-purified polyclonal antibody (Catalog # AF3240). Tissue was stained using anti- goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

Human/Mouse Biotin 162531 Rat IgG2A BAM11201 100 µg $240 Frizzled-1 None Polyclonal Goat IgG AF1120 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1120 50 µg $380

Frizzled-1 Expression in Mouse Intestine. Friz­ zled-1 was detected in a cryostat tissue section of embryonic mouse intestine (E13) using anti-mouse Frizzled-1 affinity-purified polyclonal antibody (Cata- log # AF1120). Tissues were stained using the anti- goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

None 169310 Rat IgG2A MAB1001-050 50 µg $150 Human/Mouse

None 169310 Rat IgG2A MAB1001-100 100 µg $240 Frizzled-3 None Polyclonal Goat IgG AF1001 100 µg $355 Mouse

Biotin 169310 Rat IgG2A BAM1001 100 µg $295

Biotin 145901 Rat IgG2A BAM194 100 µg $240

Human/Mouse None 145901 Rat IgG2A MAB194-050 50 µg $150 Frizzled-4

None 145901 Rat IgG2A MAB194-100 100 µg $240 Mouse None Polyclonal Goat IgG AF194 100 µg $355 Frizzled-6 Mouse None Polyclonal Goat IgG AF1526 100 µg $355

None 151143 Rat IgG2A MAB1981-050 50 µg $150 Human/Mouse

Frizzled-7 None 151143 Rat IgG2A MAB1981-100 100 µg $240 Mouse None Polyclonal Goat IgG AF198 100 µg $355 Frizzled-8 Mouse None Polyclonal Goat IgG AF112 100 µg $355 Frizzled-9 Mouse None Polyclonal Goat IgG AF2440 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

Human None 393812 Rat IgG1 MAB3766 100 µg $240 Frk aa 1-107 Human/Mouse/Rat None Polyclonal Goat IgG AF3766 100 µg $355

Frk in Human Breast Cancer. Fyn-related kinase (Frk) was detected in paraffin-embedded human breast cancer sections using anti-human Frk mono- clonal antibody (Catalog # MAB3766). Tissue was sub- jected to antigen retrieval using Antigen Retrieval Kit (Catalog # CTS013; brown). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematox- ylin (blue).

FRS2 Human None Polyclonal Sheep IgG AF4069 100 µg $355 Fucosyltransferase 8/FUT8 Human None Polyclonal Sheep IgG AF5768 100 µg $355

Furin Human None 222728 Mouse IgG1 MAB15031 500 µg $325

FXR/NR1H4 Human None A9033A Mouse IgG2A PP-A9033A-00 100 µg $380 Fyn Human None Polyclonal Goat IgG AF3574 100 µg $355

G9a/EHMT2 Human/Mouse None A8620A Mouse IgG2A PP-A8620A-00 100 µg $380 GABAA R d N-Terminus Mouse/Rat None Polyclonal Rabbit IgG PPS090 100 µL $355 GABAB R2 N-Terminus Rat None Polyclonal Goat IgG AF1188 100 µg $355 None Polyclonal Goat IgG AF2086 100 µg $355 GAD1/GAD67 aa 2-97 Human

None 252910 Mouse IgG1 MAB2086 100 µg $240 GAD2/GAD65 aa 2-150 Human None Polyclonal Goat IgG AF2247 100 µg $355 Human None Polyclonal Goat IgG AF1152 100 µg $355 None Polyclonal Goat IgG AF1245 100 µg $355 Galectin-1 Biotin Polyclonal Goat IgG BAF1245 50 µg $380 Mouse

Biotin 201002 Rat IgG2B BAM12451 250 µg $380

None 201002 Rat IgG2B MAB12451 500 µg $325 None Polyclonal Goat IgG AF1154 100 µg $355 Human

None 194804 Mouse IgG2B MAB1154 500 µg $325 Galectin-3 None Polyclonal Goat IgG AF1197 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1197 50 µg $380 Human None Polyclonal Goat IgG AF1227 100 µg $355 Galectin-4 Mouse None Polyclonal Goat IgG AF2128 100 µg $355 None Polyclonal Goat IgG AF1339 100 µg $355 Human Biotin Polyclonal Goat IgG BAF1339 50 µg $380 Galectin-7 None Polyclonal Goat IgG AF1304 100 µg $355 Mouse

None 212923 Rat IgG2A MAB1304 500 µg $325 Galectin-8 Human None Polyclonal Goat IgG AF1305 100 µg $355 None Polyclonal Goat IgG AF2045 100 µg $355 Galectin-9 Human Biotin Polyclonal Goat IgG BAF2045 50 µg $380 Galectin-9 Short Isoform Mouse None Polyclonal Goat IgG AF3535 100 µg $355 Gas1 Mouse None Polyclonal Goat IgG AF2644 100 µg $355 Gas6 Human None Polyclonal Goat IgG AF885 100 µg $355

GASP-1/WFIKKNRP Human None 288518 Mouse IgG2B MAB20701 100 µg $240

GASP-2/WFIKKN Human None 289802 Mouse IgG2B MAB21363 100 µg $240

GATA-1 Human None 234732 Rat IgG2B MAB1779 100 µg $240 None Polyclonal Goat IgG AF2046 100 µg $355 GATA-2 Human Biotin Polyclonal Goat IgG BAF2046 50 µg $380

GATA-3 Human None 291106 Mouse IgG1 MAB26051 100 µg $240 None Polyclonal Goat IgG AF2605 100 µg $355 GATA-3 aa 135-258 Human Biotin Polyclonal Goat IgG BAF2605 50 µg $380

52 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF2606 100 µg $355 GATA-4 Human Biotin Polyclonal Goat IgG BAF2606 50 µg $380

GATA-4 in Differentiated Human Embryonic Stem Cells. GATA-4 was detected in differentiated human Embryonic Stem cells using anti-human GATA-4 poly- clonal antibody (Catalog # AF2606; green). The cells were counterstained with DAPI (blue). Courtesy of Dr. Frank Solder from the National Institute of Neurological Disorders and Stroke & Stem Cell Unit at NIH.

GATA-5 Human None Polyclonal Goat IgG AF2170 100 µg $355 None Polyclonal Goat IgG AF1700 100 µg $355 GATA-6 Human Biotin Polyclonal Goat IgG BAF1700 50 µg $380

None 222228 Mouse IgG1 MAB1700 100 µg $240 GCNF/NR6A1 Human None Polyclonal Goat IgG AF3180 100 µg $355 GDF-3 Mouse None Polyclonal Goat IgG AF958 100 µg $355 None Polyclonal Goat IgG AF853 100 µg $355 GDF-5 Mouse Biotin Polyclonal Goat IgG BAF853 50 µg $380

Biotin 90509 Rat IgG2A BAM853 50 µg $380 None Polyclonal Goat IgG AF788 100 µg $355 GDF-8/Myostatin Mouse Biotin Polyclonal Goat IgG BAF788 50 µg $380 None Polyclonal Goat IgG AF739 100 µg $355 GDF-9 Mouse Biotin Polyclonal Goat IgG BAF739 50 µg $380 GDNF Human/Rat None Polyclonal Goat IgG AF-212-NA 100 µg $355 None Polyclonal Sheep IgG AF2594 100 µg $355 GFAP Human Biotin Polyclonal Sheep IgG BAF2594 50 µg $380

None 273807 Mouse IgG1 MAB2594 100 µg $240 GFAP in Rat Astrocytes. Glial fibrillary acidic protein (GFAP) was detected in astrocytes in differentia­ ting cultures of rat cortical stem cells using anti- human GFAP affinity-purified polyclonal antibody (Catalog # AF2594). The cells were stained using NorthernLights-557 anti-sheep secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

GFI-1 Human None Polyclonal Goat IgG AF3540 100 µg $355 Human None Polyclonal Goat IgG AF714 100 µg $355 None Polyclonal Goat IgG AF560 100 µg $355 GFRa-1/GDNF Ra-1 Rat Biotin Polyclonal Goat IgG BAF560 50 µg $380

None 81401 Mouse IgG2B MAB560 500 µg $325 GFRa-1 and GDNF Expression in Rat Dorsal Root Ganglia. GFRa-1 and GDNF were detected in rat dor- sal root ganglia using biotinylated anti-rat GFRa-1 affinity-purified polyclonal antibody (Catalog # BAF560; red) and anti-human GDNF affinity- purified polyclonal antibody (Catalog # AF-212-NA; green).

Human None Polyclonal Goat IgG AF613 100 µg $355 GFRa-2/GDNF Ra-2 Mouse None Polyclonal Goat IgG AF429 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Human None Polyclonal Goat IgG AF670 100 µg $355 GFRa-3/GDNF Ra-3 None Polyclonal Goat IgG AF2645 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF2645 50 µg $380

GFRa-3 in Mouse Embryonic Dorsal Root Gangli- on (DRG). Detection of Glial cell line-derived Factor Receptor alpha 3 (GFRa-3) in a cryostat section of E15 DRG mouse using anti-mouse GFRa-3 affinity-puri- fied polyclonal antibody (Catalog # AF2645). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS008 and counterstained with hematoxylin (blue).

None Polyclonal Goat IgG AF1677 100 µg $355 GFRa-4/GDNF Ra-4 Mouse Biotin Polyclonal Goat IgG BAF1677 50 µg $380

None 171217 Rat IgG2A MAB1677 500 µg $325 GITR/TNFRSF18 Human None Polyclonal Goat IgG AF689 100 µg $355

GLI-1 Human/Mouse None 388516 Rat IgG2A MAB3324 100 µg $240 GLI-1 aa 1-234 Human None Polyclonal Goat IgG AF3324 100 µg $355 GLI-1 aa 1-237 Human/Mouse None Polyclonal Goat IgG AF3455 100 µg $355 None Polyclonal Sheep IgG AF3526 100 µg $355 GLI-2 Human Biotin Polyclonal Sheep IgG BAF3526 50 µg $380 GLI-2 aa 1-416 Mouse None Polyclonal Goat IgG AF3635 100 µg $355 GLI-3 aa 1-479 Human/Mouse None Polyclonal Goat IgG AF3690 100 µg $355

GLP/EHMT1 Human/Mouse None B0422 Mouse IgG2A PP-B0422-00 100 µg $380

GLP-1R Human None 419208 Mouse IgG2A MAB28141 100 µg $240

GLP-2R Human None 413801 Mouse IgG2A MAB4285 100 µg $240

Glucagon Human/Mouse None 181402 Mouse IgG2A MAB1249 100 µg $240 Glucagon in bTC6 Cells. Glucagon expression was detected in bTC6 mouse insulinoma cells using anti- human/mouse Glucagon monoclonal antibody (Catalog # MAB1249). The cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody ( Catalog # NL007; red) and counterstained with DAPI (blue).

Glucagon NorthernLights-conjugated Human/Mouse NL557 181402 Mouse IgG2A NL1249R 500 µL $345

Glut1 Human None 202915 Mouse IgG2B MAB1418 200 µg $270

Human None 199017 Mouse IgG2A MAB1414 200 µg $270

Glut2 Biotin 205115 Rat IgG2B BAM1440 200 µg $380 Mouse

None 205115 Rat IgG2B MAB1440 200 µg $270

Biotin 202017 Mouse IgG2B BAM1415 200 µg $315 Glut3 Human

None 202017 Mouse IgG2B MAB1415 200 µg $270

Biotin 1F8 Mouse IgG1 BAM1262 250 µg $380 Glut4 Rat

None 1F8 Mouse IgG1 MAB1262 200 µg $270

Biotin 195205 Mouse IgG2A BAM1349 200 µg $380 Glut5 Human

None 195205 Mouse IgG2A MAB1349 200 µg $270

54 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Glutathione Peroxidase 1 Human/Mouse/Rat None Polyclonal Goat IgG AF3798 100 µg $355

GPX1 in Rat Liver. Glutathione Peroxidase 1 (GPX1) was detected in a cryostat section of rat liver using anti-human/mouse/rat GPX1 affinity-purified poly- clonal antibody (Catalog # AF3798). The tissue was stained using the anti-goat Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Glutathione Peroxidase 3 aa 74-226 Human/Mouse/Rat None Polyclonal Goat IgG AF4199 100 µg $355

GPX3 in Human Kidney. Glutathione Peroxidase 3 (GPX3) was detected in paraffin-embedded human kidney sections using anti-human GPX3 affinity-puri- fied polyclonal antibody (Catalog # AF4199). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counter- stained with hematoxylin (blue).

Glycine R Pan Specific Rat None Polyclonal Rabbit IgG PPS009 200 µg $355

Biotin R10 Mouse IgG1 BAM12281 250 µg $380 Glycophorin A Human

None R10 Mouse IgG1 MAB1228 500 µg $325 Glypican 1 Human None Polyclonal Goat IgG AF4519 100 µg $355 Human None Polyclonal Goat IgG AF2304 100 µg $355 None Polyclonal Goat IgG AF2355 100 µg $355 Glypican 2 Mouse Biotin Polyclonal Goat IgG BAF2355 50 µg $380

None 300736 Rat IgG2A MAB2355 100 µg $240 Glypican 3 Human None Polyclonal Sheep IgG AF2119 100 µg $355 Glypican 6 Mouse None Polyclonal Goat IgG AF1053 100 µg $355 None Polyclonal Goat IgG AF1546 100 µg $355 Canine GM-CSF Biotin Polyclonal Goat IgG BAF1546 50 µg $380

Human None 3209 Mouse IgG1 MAB215 500 µg $325 GMF-b Human None Polyclonal Goat IgG AF1276 100 µg $355 Goosecoid Human None Polyclonal Goat IgG AF4086 100 µg $355

Biotin 125623 Rat IgG2A BAM4681 100 µg $240 gp130 Mouse

None 125623 Rat IgG2A MAB4681 100 µg $240 gp130 in Mouse Splenocytes. gp130 was detected in mouse splenocytes using anti-mouse gp130 monoclonal antibody (Catalog # MAB4681; red). Cells were counterstained with DAPI (blue).

GPR34 Human None 419859 Mouse IgG2A MAB4617 100 µg $240

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

GPR50 Human None 461129 Mouse IgG2A MAB4645 100 µg $240 GPR50 in Human Glioblastoma Cells. G protein- coupled receptor 50 (GPR50) was detected in human A172 glioblastoma cell line using anti-human GPR50 monoclonal antibody (Catalog # MAB4645). Cells were stained using NorthernLights-557 donkey anti- mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

GPRC5A Human None 481906 Mouse IgG2A MAB5239 100 µg $240

None H8004 Mouse IgG2A PP-H8004-00 100 µg $380 GR/NR3C1 Human

None H8031 Mouse IgG2A PP-H8031-00 100 µg $380

Biotin RB6-8C5 Rat IgG2B BAM1037 250 µg $380 Gr-1/Ly-6G Mouse

None RB6-8C5 Rat IgG2B MAB1037 500 µg $325

Granzyme A Human None 356412 Mouse IgG2B MAB29051 100 µg $240 Granzyme A in Human Peripheral Blood Mononu- clear Cells. Granzyme A was detected in human peripheral blood mononuclear cells using anti- human Granzyme A monoclonal antibody (Catalog # MAB29051). Cells were stained using donkey anti- mouse NorthernLights-557 secondary antibody (Catalog # NL007; red) and counterstained green.

Human None 351927 Mouse IgG2A MAB2906 100 µg $240 Granzyme B None Polyclonal Goat IgG AF1865 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1865 50 µg $380 Granzyme D Mouse None Polyclonal Goat IgG AF1365 100 µg $355 None Polyclonal Goat IgG AF1377 100 µg $355 Granzyme H Human Biotin Polyclonal Goat IgG BAF1377 50 µg $380 GRB2 Human None Polyclonal Goat IgG AF3846 100 µg $355

GRB2 in Human Breast Cancer. Growth Factor Re- ceptor Bound Protein 2 (GRB2) was detected in paraf- fin-embedded human breast cancer sections using anti-human/mouse/rat GRB2 affinity-purified poly- clonal antibody (Catalog # AF3846). Tissue was sub- jected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Cata- log # CTS008; brown). Tissue was counterstained with hematoxylin (blue).

None Polyclonal Goat IgG AF956 100 µg $355 Gremlin Mouse Biotin Polyclonal Goat IgG BAF956 50 µg $380 GRK2 aa 468-689 Human/Mouse/Rat None Polyclonal Sheep IgG AF4339 100 µg $355 None Polyclonal Goat IgG AF1067 100 µg $355 Human Biotin Polyclonal Goat IgG BAF1067 50 µg $380 Growth Hormone None Polyclonal Goat IgG AF1566 100 µg $355 Rat Biotin Polyclonal Goat IgG BAF1566 50 µg $380

None 222517 Mouse IgG2B MAB15661 500 µg $325

56 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF1210 100 µg $355 Human Biotin Polyclonal Goat IgG BAF1210 50 µg $380 Growth Hormone R/GHR None Polyclonal Goat IgG AF1360 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1360 50 µg $380 Rat None Polyclonal Goat IgG AF1211 100 µg $355

GRP75/HSPA9B Human None 419605 Mouse IgG1 MAB35841 100 µg $240 GRP75 in Human Fibroblastic Meningioma. Glu- cose Regulated 75 kDa Protein (GRP75) was detected in a paraffin-embedded human fibroblastic meningi- oma section using anti-human GRP75 monoclonal antibody (Catalog # MAB35841). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Stain- ing Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

GRP75/HSPA9B aa 542-679 Human/Mouse/Rat None Polyclonal Goat IgG AF3584 100 µg $355

GSK-3a aa 90-483 Human None 288901 Mouse IgG2B MAB2986 100 µg $240 Phospho-GSK-3a (S21) Human/Mouse/Rat None Polyclonal Rabbit IgG AF4125 100 µg $285

Phosphorylated GSK-3a in Human Thymus. Phos- phorylated Glycogen Synthase kinase-3a (GSK-3a) was detected in paraffin-embedded human thymus tissue sections using anti-human/mouse/rat Phos- pho-GSK-3a (S27) affinity-purified poly­clonal anti- body (Catalog # AF4125). Tissues were stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Cat- alog # CTS005; brown) and counterstained with he- matoxylin (blue).

Phospho-GSK-3a/b (S21/S9) Human/Mouse/Rat None Polyclonal Rabbit IgG AF1590 100 µg $285

GSK-3b Human None 272535 Rat IgG2B MAB25061 100 µg $240 None Polyclonal Goat IgG AF1141 100 µg $355 HAI-1 aa 30-433 Mouse Biotin Polyclonal Goat IgG BAF1141 50 µg $380 None Polyclonal Goat IgG AF1048 100 µg $355 HAI-1 Ectodomain Human Biotin Polyclonal Goat IgG BAF1048 50 µg $380 None Polyclonal Goat IgG AF1107 100 µg $355 HAI-2 Ectodomain Mouse Biotin Polyclonal Goat IgG BAF1107 50 µg $380 Hairless Human None Polyclonal Goat IgG AF5708 100 µg $355 None Polyclonal Goat IgG AF3168 100 µg $355 HAND1 Human Biotin Polyclonal Goat IgG BAF3168 50 µg $380 HAND2 Human/Mouse None Polyclonal Goat IgG AF3876 100 µg $355 HAPLN4 Human None Polyclonal Goat IgG AF4085 100 µg $355 HB-EGF Human None Polyclonal Goat IgG AF-259-NA 250 µg $355

None 456738 Mouse IgG2A MAB5246 100 µg $240 HCRTR2 Human

None 456723 Mouse IgG2B MAB52461 100 µg $240 None Polyclonal Goat IgG AF1105 100 µg $355 Hepassocin Rat Biotin Polyclonal Goat IgG BAF1105 50 µg $380 HES-1 aa 93-280 Human None Polyclonal Goat IgG AF3317 100 µg $355 HES-4 aa 94-221 Human None Polyclonal Sheep IgG AF3600 100 µg $355 Human None Polyclonal Goat IgG AF-294-NA 100 µg $355 HGF Mouse None Polyclonal Goat IgG AF2207 100 µg $355 HGF Activator Human None Polyclonal Goat IgG AF1514 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF276 100 µg $355 Human

None 95308 Mouse IgG1 MAB3583 100 µg $240 HGF R/c-MET None Polyclonal Goat IgG AF527 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF527 50 µg $380 Phospho-HGF R/c-MET (Y1003) Human None Polyclonal Rabbit IgG AF4059 100 µg $285 Phospho-HGF R/c-MET (Y1234/Y1235) Human None Polyclonal Rabbit IgG AF2480 50 µg $285 Phospho-HGF R/c-MET (Y1349) Human None Polyclonal Rabbit IgG AF3950 50 µg $285

HIF-1a aa 575-826 Human/Mouse None 241812 Mouse IgG1 MAB1935 100 µg $240 HIF-2a aa 542-874 Mouse None Polyclonal Goat IgG AF2997 100 µg $355 Phospho-Histone H2AX (S139) Human None Polyclonal Rabbit IgG AF2288 50 µg $285 HMGA2 Human None Polyclonal Goat IgG AF3184 100 µg $355 HNF-3b/FoxA2 aa 242-457 Human None Polyclonal Goat IgG AF2400 100 µg $355

HNF-3b in Human BG01V Cells. Hepatic Nuclear Factor 3 b (HNF-3b) was detected in endoderm dif- ferentiated human BG01V embryonic stem cell line using anti-human HNF-3b affinity-purified polyclonal antibody (Catalog # AF2400). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

None H1415 Mouse IgG2A PP-H1415-00 100 µg $380 HNF-4a/NR2A1 Human

None K9218 Mouse IgG2A PP-K9218-00 100 µg $380

HNF-4g/NR2A2 Human None N3224 Mouse IgG2A PP-N3224-00 100 µg $380

HNF-4a/NR2A1 Isoform 7-9 Human None H6939 Mouse IgG1 PP-H6939-00 100 µg $380 HOXA1 Human None Polyclonal Goat IgG AF5014 100 µg $355

HSP60 Human/Mouse/Rat None 264233 Mouse IgG2B MAB1800 100 µg $240

HSP70 Human/Mouse None 242707 Mouse IgG2A MAB1663 100 µg $240 HSPH1 aa 595-733 Human/Mouse/Rat None Polyclonal Goat IgG AF4029 100 µg $355

HSPH1 in Rat Cerebellum. HSP (Heat Shock Protein) H1 was detected in a cryostat section of rat cerebel- lum using anti-human/mouse/rat HSPHI affinity- purified polyclonal antibody (Catalog # AF4029). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Human None 229926 Mouse IgG2B MAB1458 100 µg $240 HTRA2/Omi Human/Mouse/Rat None Polyclonal Rabbit IgG AF1458 100 µg $285

HVEM/TNFRSF14 Human None 94804 Mouse IgG1 MAB3561 500 µg $325 cIAP-1/HIAP-2 Human None Polyclonal Goat IgG AF8181 100 µg $355 None Polyclonal Goat IgG AF8171 100 µg $355 cIAP-2/HIAP-1 Human

None 315304 Mouse IgG1 MAB817 100 µg $240 ICA1 Human None Polyclonal Goat IgG AF4084 100 µg $355 None Polyclonal Goat Serum BBA17 500 µL $195

Human None BBIG-I1 Mouse IgG1 BBA3 100 µg $230

None BBIG-I1 Mouse IgG1 BBA4 1 mg $840 ICAM-1/CD54 None Polyclonal Goat IgG AF796 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF796 50 µg $380 Rat None Polyclonal Goat IgG AF583 100 µg $355

58 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF774 100 µg $355 ICAM-2/CD102 Mouse Biotin Polyclonal Goat IgG BAF774 50 µg $380

ICAM-3/CD50 Human None ICAM-3.3 Mouse IgG1 BBA15 200 µg $315

ICAM-5 Human None 283607 Mouse IgG2A MAB1950 100 µg $240 None Polyclonal Goat IgG AF1173 100 µg $355 ICAM-5 Mouse Biotin Polyclonal Goat IgG BAF1173 50 µg $380 ICOS Human None Polyclonal Goat IgG AF169 100 µg $355 ID2 Human None Polyclonal Goat IgG AF4660 100 µg $355 IFITM1 Human None Polyclonal Goat IgG AF4827 100 µg $355 IFITM3/Fragilis Mouse None Polyclonal Goat IgG AF3377 100 µg $355

IFITM3/Fragilis in Embryonic Mouse Ovary. Inter- feron-induced transmembrane protein 3 (IFITM3) was detected in an E13.5 mouse embryonic ovary section using anti-mouse IFITM3 affinity-purified polyclonal antibody (Catalog # AF3377). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

None MMHA-3 Mouse IgG1 21101-1 50 µg $195

None MMHA-1 Mouse IgG1 21105-1 50 µg $195

None MMHA-8 Mouse IgG2B 21110-1 50 µg $195

None MMHA-11 Mouse IgG1 21112-1 50 µg $250

Fluorescein MMHA-11 Mouse IgG1 21112-3 50 Tests $300

None MMHA-13 Mouse IgG1 21116-1 50 µg $195

Human None MMHA-17 Mouse IgG1 21118-1 50 µg $195

None MMHA-6 Mouse IgG1 21125-1 50 µg $195

IFN-a None MMHA-9 Mouse IgG1 21127-1 50 µg $195

None MMHA-14 Mouse IgG1 21129-1 50 µg $195 None Polyclonal Sheep Serum 31100-1 100000 U $235 None Polyclonal Rabbit Serum 31101-1 100000 U $235 None Polyclonal Rabbit Serum 31130-1 20000 U $215

Fluorescein RMMA-1 Rat IgG1 22100-3 100 µL $355 Mouse None Polyclonal Rabbit Serum 32100-1 20000 U $325

None K9 Mouse IgG1 27100-1 500 µg $315 Porcine

None F17 Mouse IgG1 27105-1 500 µg $315 IFN-a/b R1 Mouse Biotin Polyclonal Goat IgG BAF3039 50 µg $380

a b Human/Mouse/Rat/Bovine/ None Polyclonal Rabbit IgG PPS074 100 µL $355 Phospho-IFN- / R1 (S535/S539) Canine/Primate/Sheep

None MMHAR-2 Mouse IgG2A 21385-1 50 µg $330 IFN-a/b R2 Human None Polyclonal Rabbit Serum 31385-1 50 µg $250

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

Fluorescein MMHB-3 Mouse IgG1 21400-3 50 Tests $270

None MMHB-1 Mouse IgG2A 21405-1 50 µg $195

None MMHB-12 Mouse IgG1 21450-1 50 µg $195 None MMHB-13 Mouse IgG3 21455-1 50 µg $195

Human None MMHB-14 Mouse IgG2A 21460-1 50 µg $195

IFN-b None MMHB-15 Mouse IgG2B 21465-1 50 µg $195

None MMHB-16 Mouse IgG1 21470-1 50 µg $195 None Polyclonal Sheep Serum 31400-1 20000 U $280 None Polyclonal Rabbit Serum 31410-1 20000 U $225

Fluorescein RMMB-1 Rat IgG1 22400-3 100 µL $320 Mouse None Polyclonal Rabbit Serum 32400-1 20000 U $315 None Polyclonal Goat IgG AF2300 100 µg $355 Bovine

None 345025 Rat IgG2A MAB2300 100 µg $240 None Polyclonal Goat IgG AF781 100 µg $355 Canine Biotin Polyclonal Goat IgG BAF781 50 µg $380 Equine None Polyclonal Goat IgG AF1586 100 µg $355 None Polyclonal Goat IgG AF-285-NA 100 µg $355 IFN-g Biotin Polyclonal Goat IgG BAF285 50 µg $380 Human

None 25718 Mouse IgG2A MAB285 500 µg $325

None 25723 Mouse IgG2B MAB2851 500 µg $325 Mouse None H22 Hamster IgG MAB4851 500 µg $325 Porcine Biotin Polyclonal Goat IgG BAF985 50 µg $380 Rat None Polyclonal Goat IgG AF-585-NA 100 µg $355

IFN-g in Human Peripheral Blood Mononuclear Cells (PBMC). Interferon g (IFN-g) was detected in PMA-, ionomyocin-, and monensin-activated human PBMC using anti-human IFN-g affinity-purified poly- clonal antibody (Catalog # AF-285-NA). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

IFN-g R1/CD119 Human None Polyclonal Goat IgG AF673 100 µg $355 NL557 Polyclonal Donkey IgG NL001 500 µL $115 Goat NL637 Polyclonal Donkey IgG NL002 500 µL $115 NL493 Polyclonal Donkey IgG NL003 500 µL $115 NL557 Polyclonal Donkey IgG NL007 500 µL $115 Mouse NL637 Polyclonal Donkey IgG NL008 500 µL $115 NL493 Polyclonal Donkey IgG NL009 500 µL $115 NL557 Polyclonal Donkey IgG NL004 500 µL $115 IgG NorthernLights-conjugated Rabbit NL637 Polyclonal Donkey IgG NL005 500 µL $115 NL493 Polyclonal Donkey IgG NL006 500 µL $115 NL557 Polyclonal Goat IgG NL013 500 µL $115 Rat NL637 Polyclonal Goat IgG NL014 500 µL $115 NL493 Polyclonal Goat IgG NL015 500 µL $115 NL557 Polyclonal Donkey IgG NL010 500 µL $115 Sheep NL637 Polyclonal Donkey IgG NL011 500 µL $115 NL493 Polyclonal Donkey IgG NL012 500 µL $115

IgG2A Mouse None 133304 Mouse IgG2A MAB0031 500 µg $170

IgG2B Mouse None 73009 Mouse IgG2B MAB0042 500 µg $170

60 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE NL557 Polyclonal Goat IgG NL016 500 µL $115 IgY NorthernLights-conjugated Chicken NL637 Polyclonal Goat IgG NL017 500 µL $115 NL493 Polyclonal Goat IgG NL018 500 µL $115 IGFBP-1 Mouse None Polyclonal Goat IgG AF1240 100 µg $355 None Polyclonal Goat IgG AF675 100 µg $355 IGFBP-3 Human

None 84728 Mouse IgG2B MAB305 500 µg $325 None Polyclonal Goat IgG AF804 100 µg $355 IGFBP-4 Human Biotin Polyclonal Goat IgG BAF804 50 µg $380 None Polyclonal Goat IgG AF875 100 µg $355 Human

IGFBP-5 None 164503 Mouse IgG2B MAB875 500 µg $325 Mouse None Polyclonal Goat IgG AF578 100 µg $355

IGFBP-5 in Mouse Ovary. Insulin-like Growth Factor Binding Protein 5 (IGFBP-5) was detected in a cryostat section of mouse ovary (follicle with oocyte) using anti-mouse IGFBP-5 affinity-purified polyclonal anti- body (Catalog # AF578). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

IGFBP-6 Mouse None Polyclonal Goat IgG AF776 100 µg $355 None Polyclonal Goat IgG AF4130 100 µg $355 IGFBP-L1 Mouse Biotin Polyclonal Goat IgG BAF4130 50 µg $380 None Polyclonal Goat IgG AF1334 100 µg $355 Human IGFBP-rp1/IGFBP-7 Biotin Polyclonal Goat IgG BAF1334 50 µg $380

Mouse None 292917 Rat IgG1 MAB21201 100 µg $240 Human None Polyclonal Goat IgG AF-291-NA 250 µg $355 IGF-I Mouse None Polyclonal Goat IgG AF791 100 µg $355 IGF-I R Human None Polyclonal Goat IgG AF-305-NA 100 µg $355 Human None Polyclonal Goat IgG AF-292-NA 250 µg $355 IGF-II Mouse None Polyclonal Goat IgG AF792 100 µg $355 IGF-II R Human None Polyclonal Goat IgG AF2447 100 µg $355

IGSF2/CD101 Mouse Biotin 307707 Rat IgG2A BAM3368 100 µg $380 IGSF4B Human None Polyclonal Goat IgG AF3678 100 µg $355

None 404619 Rat IgG1 MAB4164 100 µg $240 IGSF4C Human

None 404617 Rat IgG1 MAB41641 100 µg $240 None Polyclonal Goat IgG AF3117 100 µg $355 IGSF8 Mouse Biotin Polyclonal Goat IgG BAF3117 50 µg $380 Ikaros Human None Polyclonal Goat IgG AF4984 100 µg $355 IKKa aa 375-745 Human/Mouse/Rat None Polyclonal Sheep IgG AF3768 100 µg $355 IKKg Human/Mouse/Rat None Polyclonal Goat IgG AF2684 100 µg $355 Human None Polyclonal Goat IgG AF3199 100 µg $355 IKKe

Human/Mouse/Rat None 346909 Mouse IgG1 MAB3199 100 µg $240 None Polyclonal Goat IgG AF-200-NA 100 µg $355 Human Biotin Polyclonal Goat IgG BAF200 50 µg $380 IL-1a/IL-1F1

None 4414 Mouse IgG2A MAB200 500 µg $325 Mouse None Polyclonal Goat IgG AF-400-NA 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF1796 100 µg $355 Feline Biotin Polyclonal Goat IgG BAF1796 50 µg $380 None Polyclonal Total Goat IgG AB-201-NA 1 mg $315 None Polyclonal Goat IgG AF-201-NA 100 µg $355 Human Biotin Polyclonal Goat IgG BAF201 50 µg $380

IL-1b/IL-1F2 None 8516 Mouse IgG1 MAB201 500 µg $325

None 2805 Mouse IgG1 MAB601 500 µg $325 Mouse None Polyclonal Goat IgG AF-401-NA 100 µg $355 None Polyclonal Goat IgG AF-501-NA 100 µg $355 Rat Biotin Polyclonal Goat IgG BAF501 50 µg $380

None 38139 Mouse IgG1 MAB501 500 µg $325 IL-1b in Human Peripheral Blood Mononuclear IL-1b in Rat Splenocytes. Interleukin 1b (IL-1b) was Cells (PBMCs). Interleukin-1b (IL-1b) was detected detected in LPS-stimulated rat splenocytes using in LPS-stimulated human PBMCs using anti-human anti-rat IL-1b affinity-purified polyclonal antibody IL-1b affinity-purified polyclonal antibody (Catalog (Catalog # AF-501-NA). Cells were stained using # AF-201-NA). The cells were stained using NorthernLights-557 anti-goat IgG secondary anti- NorthernLights-557 anti-goat secondary antibody body (Catalog # NL001; red) and counterstained (Catalog # NL001; red) and counterstained with DAPI (green). (blue).

IL-1b/IL-1F2 NorthernLights-conjugated Human NL557 Polyclonal Goat IgG NL201R 500 µL $350 Equine None Polyclonal Goat IgG AF2466 100 µg $355 IL-1ra/IL-1F3 Human None Polyclonal Goat IgG AF-280-NA 100 µg $355 Porcine None Polyclonal Goat IgG AF780 100 µg $355 IL-1F5/FIL1d Human None Polyclonal Goat IgG AF1275 100 µg $355 None Polyclonal Goat IgG AF1078 100 µg $355 IL-1F6/FIL1e Human Biotin Polyclonal Goat IgG BAF1078 50 µg $380 None Polyclonal Goat IgG AF1099 100 µg $355 IL-1F8/FIL1η Human Biotin Polyclonal Goat IgG BAF1099 50 µg $380 IL-1 RI Mouse None Polyclonal Goat IgG AF771 100 µg $355 None Polyclonal Goat IgG AF-263-NA 100 µg $355 Human

IL-1 RII None 32437 Mouse IgG2A MAB263 500 µg $325 Mouse None Polyclonal Goat IgG AF563 100 µg $355

IL-1 RII in Human Peripheral Blood Mononuclear Cells (PBMCs). Interleukin-1 receptor II (IL-1 RII) was detected in human PBMCs by indirect immunofluo- rescent staining using anti-human IL-1 RII affinity- purified polyclonal antibody (Catalog # AF-263-NA) and counterstained (green).

IL-1 RAPL2/IL-1 R9 Human None Polyclonal Goat IgG AF1007 100 µg $355

62 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Bovine None Polyclonal Goat IgG AF2465 100 µg $355 Canine Biotin Polyclonal Goat IgG BAF1815 50 µg $380 None Polyclonal Goat IgG AF1613 100 µg $355 Equine Biotin Polyclonal Goat IgG BAF1613 50 µg $380 None Polyclonal Goat IgG AF1890 100 µg $355 Feline Biotin Polyclonal Goat IgG BAF1890 50 µg $380 IL-2 None Polyclonal Goat IgG AF-202-NA 100 µg $355 Biotin Polyclonal Goat IgG BAF202 50 µg $380 Human

None 5334 Mouse IgG1 MAB202 500 µg $325

None 5355 Mouse IgG2A MAB602 500 µg $325 None Polyclonal Goat IgG AF652 100 µg $355 Porcine Biotin Polyclonal Goat IgG BAF652 50 µg $380 Rat None Polyclonal Goat IgG AF-502-NA 100 µg $355

IL-2 in Human Peripheral Blood Mononuclear Cells (PBMCs). Interleukin-2 (IL-2) was detected in PMA- and ionomycin-stimulated human PBMCs using biotinylated anti-human IL-2 affinity-purified polyclonal antibody (Catalog # BAF202; red) and counterstained green.

None 22722 Mouse IgG1 MAB223 500 µg $325 Human

None 24204 Mouse IgG1 MAB623 500 µg $325 IL-2 Ra Mouse None Polyclonal Goat IgG AF2438 100 µg $355 Rat None Polyclonal Sheep IgG AF5156 100 µg $355

IL-2 Ra in Mouse Spleen. Interleukin-2 Receptor a (IL-2 Ra) was detected in cryostat tissue sections of mouse spleen using anti-mouse IL-2 Ra affinity-puri- fied polyclonal antibody (Catalog # AF2438). Tissues were stained using NorthernLights-557 donkey anti- goat IgG secondary antibody (Catalog # NL001; red). Tissues were counterstained green.

IL-2 Rb Human None 27302 Mouse IgG1 MAB224 500 µg $325 None Polyclonal Total Goat IgG AB-203-NA 1 mg $355 Human None Polyclonal Goat IgG AF-203-NA 100 µg $355

IL-3 None 4806 Mouse IgG1 MAB203 500 µg $325 None Polyclonal Goat IgG AF2524 100 µg $355 Rat Biotin Polyclonal Goat IgG BAF2524 50 µg $380

IL-3 Ra Human None 32703 Mouse IgG1 MAB301 500 µg $325

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Bovine None Polyclonal Goat IgG AF2469 100 µg $355 Equine None Polyclonal Goat IgG AF1809 100 µg $355 Biotin Polyclonal Goat IgG BAF204 50 µg $380 IL-4 Human

None 3007 Mouse IgG1 MAB304 500 µg $325 Porcine None Polyclonal Goat IgG AF654 100 µg $355 Rat Biotin Polyclonal Goat IgG BAF504 50 µg $380

IL-4 in Human Lymph Node. Interleukin-4 (IL-4) was detected in a cryostat tissue section of frozen human lymph node using anti-human IL-4 monoclonal anti- body (Catalog # MAB304). Tissues were stained using anti-mouse secondary antibody (brown) and coun- terstained with hematoxylin (blue).

IL-4 Ra Human None 25463 Mouse IgG2A MAB230 500 µg $325 Canine None Polyclonal Goat IgG AF1964 100 µg $355 Equine None Polyclonal Goat IgG AF2470 100 µg $355 Feline None Polyclonal Goat IgG AF1795 100 µg $355

Human None 9906 Mouse IgG1 MAB605 500 µg $325 IL-5 Mouse None Polyclonal Goat IgG AF-405-NA 100 µg $355 Porcine None Polyclonal Goat IgG AF3137 100 µg $355 None Polyclonal Goat IgG AF545 100 µg $355 Rat

None 299298 Mouse IgG1 MAB5452 100 µg $240 None Polyclonal Goat IgG AF1609 100 µg $355 Canine Biotin Polyclonal Goat IgG BAF1609 50 µg $380

None 247002 Mouse IgG2B MAB16091 500 µg $325 Equine None Polyclonal Goat IgG AF1886 100 µg $355 None Polyclonal Goat IgG AF2305 100 µg $355 Feline

None 341031 Mouse IgG2B MAB23051 100 µg $240 IL-6 None Polyclonal Goat IgG AF-206-NA 100 µg $355 Human Biotin Polyclonal Goat IgG BAF206 50 µg $380

None 1936 Mouse IgG2B MAB2061 500 µg $325 Mouse None Polyclonal Goat IgG AF-406-NA 100 µg $355

Porcine None 77830 Mouse IgG1 MAB686 500 µg $325 Rat None Polyclonal Goat IgG AF506 100 µg $355

IL-6 in Porcine Peripheral Blood Mononuclear Cells. Interleukin-6 (IL-6) was detected in porcine PBMCs using anti-porcine IL-6 monoclonal antibody (Catalog # MAB686). Cells were stained using NorthernLights-557 donkey anti-mouse IgG second- ary antibody (Catalog # NL007; red) and counter- stained with DAPI (blue).

None Polyclonal Goat IgG AF1830 100 µg $355

Biotin 255821 Rat IgG2A BAM1830 100 µg $380 IL-6 Ra Mouse

None 255821 Rat IgG2A MAB1830 500 µg $325

None 255820 Rat IgG1 MAB18301 100 µg $240

64 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

IL-7 Human None 7417 Mouse IgG1 MAB207 500 µg $325

CXCR1/IL-8 RA Human None 42705 Mouse IgG2A MAB330 500 µg $325

CXCR2/IL-8 RB Human None 48311 Mouse IgG2A MAB331 500 µg $325 Equine None Polyclonal Goat IgG AF1605 100 µg $355 Human None Polyclonal Goat IgG AF-217-NA 100 µg $355 IL-10 None Polyclonal Goat IgG AF693 100 µg $355 Porcine

None 262715 Mouse IgG2B MAB6932 100 µg $240 Rat None Polyclonal Goat IgG AF519 100 µg $355 IL-10 Ra Human None Polyclonal Goat IgG AF-274-NA 100 µg $355 None Polyclonal Goat IgG AF-219-NA 100 µg $355 IL-12 Human Biotin Polyclonal Goat IgG BAF219 100 µg $380

None 24910 Mouse IgG1 MAB219 500 µg $325

IL-12 p70 Human None 24945 Mouse IgG1 MAB611 500 µg $325 IL-12/IL-23 p40 Feline None Polyclonal Goat IgG AF1954 100 µg $355 Mouse None Polyclonal Goat IgG AF-413-NA 100 µg $355 IL-13

Rat Biotin 296202 Mouse IgG2B BAM1945 100 µg $380 IL-13 Ra1 Human None Polyclonal Goat IgG AF152 100 µg $355 Human None Polyclonal Goat IgG AF146 100 µg $355 IL-13 Ra2 Mouse None Polyclonal Goat IgG AF539 100 µg $355 Human None Polyclonal Goat IgG AF315 100 µg $355 IL-15 None Polyclonal Goat IgG AF447 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF447 50 µg $380 IL-15 Ra Human None Polyclonal Goat IgG AF247 100 µg $355 IL-16 C-terminal Peptide Human None Polyclonal Goat IgG AF-316-PB 100 µg $355 IL-17 Human None Polyclonal Goat IgG AF-317-NA 100 µg $355 None Polyclonal Goat IgG AF1207 100 µg $355 Human IL-17B R Biotin Polyclonal Goat IgG BAF1207 50 µg $380 Mouse None Polyclonal Goat IgG AF1040 100 µg $355 IL-17C Human None Polyclonal Goat IgG AF1234 100 µg $355 None Polyclonal Goat IgG AF1504 100 µg $355 IL-17D Human

None 246018 Mouse IgG2B MAB15041 100 µg $240 IL-17D in Human Heart. Interleukin-17D (IL-17D) was detected in a paraffin-embedded human heart section using anti-human IL-17D monoclonal anti- body (Catalog # MAB15041). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Stain- ing Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Human None Polyclonal Goat IgG AF1335 100 µg $355 IL-17F Mouse None Polyclonal Goat IgG AF2057 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Human None Polyclonal Goat IgG AF2275 100 µg $355 IL-17 RD/SEF None Polyclonal Goat IgG AF2276 100 µg $355 Mouse

None 400210 Rat IgG2A MAB2276 100 µg $240 IL-17 RD in Mouse Liver. Interleukin-17 Receptor D IL-17 RD in Human Kidney. Interleukin-17 Receptor (IL-17 RD) was detected in frozen mouse liver sec- D (IL-17 RD) was detected in a paraffin-embedded tions using anti-mouse IL-17 RD monoclonal anti- human kidney sections using anti-human IL-17 RD body (Catalog # MAB2276; red) and counterstained affinity-purified polyclonal antibody (Catalog # green. AF2275). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

None Polyclonal Goat IgG AF2924 100 µg $355 Canine

None 314811 Mouse IgG1 MAB29241 100 µg $240 IL-18/IL-1F4 Porcine None Polyclonal Goat IgG AF588 100 µg $355 Rhesus Macaque None Polyclonal Goat IgG AF2548 100 µg $355 None Polyclonal Goat IgG AF840 100 µg $355 IL-18 Ra/IL-1 R5 Human

None 70625 Mouse IgG1 MAB840 500 µg $325 IL-18 R in Human Skin. Interleukin-18 Receptor (IL- 18 R) was detected in frozen sections of human skin using anti-human IL-18 R affinity-purified polyclonal antibody (Catalog # AF840). Tissue was stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with he- matoxylin (blue).

IL-20 Ra Human None 173707 Mouse IgG1 MAB11761 500 µg $325 IL-21 Mouse None Polyclonal Goat IgG AF594 100 µg $355

IL-21 in Mouse Splenocytes. Interleukin-21 (IL-21) was detected in mouse splenocytes stimulated with concanavalin A using anti-mouse IL-21 affinity-puri- fied polyclonal antibody (Catalog # AF594). Cells were stained using NorthernLights-557 anti-goat secondary antibody (Catalog # NL001; red) and coun- terstained green.

IL-21 R Subunit Mouse None Polyclonal Goat IgG AF596 100 µg $355 IL-22 Human None Polyclonal Goat IgG AF782 100 µg $355

Human None 305405 Mouse IgG1 MAB2770 100 µg $240 IL-22 Ra1

Mouse None 496514 Rat IgG2A MAB42941 100 µg $240

Human None 283123 Mouse IgG2B MAB19651 100 µg $240 IL-24 None Polyclonal Goat IgG AF2786 100 µg $355 Mouse

Biotin 303308 Rat IgG2A BAM2786 100 µg $380 IL-27 p28 Mouse None Polyclonal Goat IgG AF1834 100 µg $355 IL-28A/IFN-λ2 Human None Polyclonal Goat IgG AF1587 100 µg $355 None Polyclonal Goat IgG AF2824 100 µg $355 IL-31 Human

None 308202 Mouse IgG1 MAB28241 100 µg $240 IL-31 RA Human None Polyclonal Goat IgG AF2769 100 µg $355

66 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF3625 100 µg $355 Human

IL-33 None 390412 Rat IgG2B MAB3625 100 µg $240 Mouse None Polyclonal Goat IgG AF3626 100 µg $355 Human None Polyclonal Goat IgG AF4810 100 µg $355 IL-33 Propeptide Mouse None Polyclonal Sheep IgG AF5010 100 µg $355

IL-33 Propeptide in Human Umbilical Vein Endo- thelial Cells. Interleukin-33 (IL-33) Propeptide was detected in HUVECs using anti-human IL-33 Propep- tide affinity-purified polyclonal antibody (Catalog # AF4810). Cells were stained using Northern- Lights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Indian Hedgehog N-Terminus Mouse None Polyclonal Goat IgG AF1705 100 µg $355 ING1 Human None Polyclonal Goat IgG AF5758 100 µg $355

Insulin Human/Mouse/Bovine None 182410 Rat IgG2A MAB1417 100 µg $240

Biotin 253627 Mouse IgG2A BAM13361 100 µg $240 Proinsulin Human/Mouse

None 253627 Mouse IgG2A MAB13361 100 µg $240 None Polyclonal Goat IgG AF1544 100 µg $355 Insulin R/CD220 Human

None 243524 Mouse IgG2B MAB1544 100 µg $240 INSRR Human None Polyclonal Goat IgG AF3055 100 µg $355 Integrin a1/CD49a Human None Polyclonal Sheep IgG AF5676 100 µg $355

Biotin HAS3 Mouse IgG2A BAM1233 100 µg $295

Human None HAS3 Mouse IgG2A MAB1233 500 µg $325

Integrin a2/CD49b None 430903 Rat IgG2A MAB12332 100 µg $240 None Polyclonal Sheep IgG AF1740 100 µg $355 Mouse Biotin Polyclonal Sheep IgG BAF1740 50 µg $380

Integrin a2b/CD41 Mouse None 386629 Rat IgG2A MAB41181 100 µg $240

Biotin IA3 Mouse IgG1 BAM1345 100 µg $295 Integrin a3/CD49c Human

None IA3 Mouse IgG1 MAB1345 500 µg $325

Biotin 7.2R Mouse IgG1 BAM1354 100 µg $295 Integrin a4/CD49d Human

None 7.2R Mouse IgG1 MAB1354 500 µg $325 Integrin a4/CD49d in Human U937 Cells. Integrin a4/CD49d was detected in human U937 lymphoma cell line using anti-human integrin a4/CD49d monoclonal antibody (Catalog # MAB1354). Cells were stained using NorthernLights-557 donkey anti- mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

Human None 238307 Mouse IgG1 MAB1864 100 µg $240 Integrin a5/CD49e

Mouse None 235112 Rat IgG2B MAB1754 100 µg $240 Integrin a5 in Human Fibrosarcoma Cell Line (HT1080). Integrin a5 was detected in HT1080 hu- man fibrosarcoma cells using anti-human integrin a5 monoclonal antibody (Catalog # MAB1219). Cells were stained using NorthernLights-557 donkey anti- mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

Human None MP4F10 Mouse IgG2B MAB1350 500 µg $325 Integrin a6/CD49f

Human/Mouse/Bovine None GoH3 Rat IgG2A MAB13501 100 µg $240 None Polyclonal Goat IgG AF4076 100 µg $355 Integrin a8 Mouse Biotin Polyclonal Goat IgG BAF4076 50 µg $380

Biotin ICRF44 Mouse IgG1 BAM1699 100 µg $240

Human None ICRF44 Mouse IgG1 MAB1699 500 µg $325

Integrin aM/CD11b None 238446 Mouse IgG2B MAB16991 100 µg $240

Biotin M1/70 Rat IgG2B BAM1124 250 µg $380 Mouse

None M1/70 Rat IgG2B MAB1124 500 µg $325 None Polyclonal Goat IgG AF1219 100 µg $355 Biotin Polyclonal Goat IgG BAF1219 50 µg $380 Integrin aV/CD51 Human

None P2W7 Mouse IgG1 MAB1219 500 µg $325

None 273210 Mouse IgG1 MAB12191 100 µg $240

Integrin aVb3 Human None 23C6 Mouse IgG1 MAB3050 100 µg $240

Integrin aVb5 Human None P5H9 Mouse IgG1 MAB2528 100 µg $240

Integrin b1/CD29 Human None 4B7R Mouse IgG1 MAB1778 100 µg $240

None P5D2 Mouse IgG1 MAB17781 100 µg $240

Human None P4G11 Mouse IgG1 MAB17782 100 µg $240

Integrin b1/CD29 None 419127 Mouse IgG2B MAB17783 100 µg $240 None Polyclonal Goat IgG AF2405 100 µg $355 Mouse

None 265917 Rat IgG2A MAB2405 100 µg $240 Integrin b1, Orexin b, and p38a in Mouse Brainstem. Cryostat sections of mouse brainstem were labeled with anti-human Orexin B monoclonal antibody (Cata- log # MAB734), anti-human/mouse/rat p38a affinity- purified polyclonal antibody (Catalog # AF8691), and anti-mouse Integrin b1 affinity-purified polyclonal anti- body (Catalog #AF2405). Tissue was stained with North- ernLights (NL)-conjugated secondary polyclonal anti- bodies: NL-493 donkey anti-rabbit (Catalog # NL006; green), NL-557 donkey anti-mouse (Catalog # NL007; red), and NL-637 donkey anti-goat (Catalog # NL002; blue). Immunohistochemical image of integrin b1 ex- pression is pseudo-colored for presentation. Integrin b2/CD18 Human None Polyclonal Goat IgG AF1730 100 µg $355 None Polyclonal Sheep IgG AF4060 100 µg $355 Human

Integrin b4/CD104 None 422325 Mouse IgG2B MAB4060 100 µg $240

Mouse None 308601 Rat IgG2A MAB4054 100 µg $240 None Polyclonal Sheep IgG AF3824 100 µg $355 Integrin b5 Human Biotin Polyclonal Sheep IgG BAF3824 50 µg $380 None Polyclonal Sheep IgG AF4155 100 µg $355 Human Biotin Polyclonal Sheep IgG BAF4155 50 µg $380

None 437211 Mouse IgG2B MAB4155 100 µg $240 Integrin b6 None Polyclonal Goat IgG AF2389 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF2389 50 µg $380

None 268622 Rat IgG2A MAB2389 100 µg $240

Integrin b7 Human None 473207 Mouse IgG1 MAB4669 100 µg $240

68 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

Integrin b8 Human None 416911 Mouse IgG1 MAB47751 100 µg $240 Integrin b8 in Human A172 Cells. Integrin b8 was detected in regions of membrane ruffling in A172 human glioblastoma cells using anti-human Integrin b8 monoclonal antibody (Catalog # MAB4775). Cells were stained using Northern- Lights-557 donkey anti-mouse IgG secondary anti- body (Catalog # NL007; red) and counterstained with DAPI (blue).

Intelectin-1/Omentin Human None Polyclonal Sheep IgG AF4254 100 µg $355

Intelectin-1 in Mouse Intestine. Intelectin-1 was detected in a section of mouse intestine using anti- mouse Intelectin-1 affinity-purified polyclonal antibody (Catalog # AF4254; red). The tissue was stained using NorthernLights-557 anti-sheep sec- ondary antibody (Catalog # NL010) and the nuclei counterstained with DAPI (blue).

IRAK4 Human None Polyclonal Goat IgG AF3919 100 µg $355 IRF3 Human None Polyclonal Goat IgG AF4019 100 µg $355 IRF5 aa 214-395 Human None Polyclonal Sheep IgG AF4508 100 µg $355 IRF8 Human None Polyclonal Sheep IgG AF5117 100 µg $355

IRS-1 Human None 395601 Mouse IgG1 MAB39781 100 µg $240

ISG15/UCRP aa 2-157 Human None 539442 Mouse IgG2B MAB4845 100 µg $240 Islet-1 Human None Polyclonal Goat IgG AF1837 100 µg $355 Islet-1 NorthernLights-conjugated Human NL557 Polyclonal Goat IgG NL1837R 500 µL $350 None Polyclonal Sheep IgG AF4244 100 µg $355 Islet-2 Human Biotin Polyclonal Sheep IgG BAF4244 50 µg $380

Islet-2 and Plexin A3 in Embryonic Mouse Spinal Cord. Islet-2 and Plexin A3 were detected in a section of embryonic mouse spinal cord using biotinylated anti-human Islet-2 affinity-purified polyclonal anti- body (Catalog # BAF4244) and anti-mouse Plexin A3 (Catalog # AF4075). The tissue was stained using NorthernLights-493 streptavidin (Catalog # NL997; green) and NorthernLights-557 donkey anti-goat IgG affinity-purified polyclonal antibody (Catalog # NL001; red), respectively. The nuclei were stained using DAPI (blue).

ISLR-2 Mouse None Polyclonal Sheep IgG AF4650 100 µg $355 Jagged 1 Human None Polyclonal Goat IgG AF1277 100 µg $355 Jagged 2 Human None Polyclonal Goat IgG AF1726 100 µg $355 Jak1 aa 14-55 Mouse/Rat None Polyclonal Goat IgG AF602 100 µg $355

Jak1 aa 20-274 Human/Mouse/Rat None 413104 Rat IgG2B MAB4260 100 µg $240 Jak1 in Human Skin. Jak1 was detected in a section of paraffin-embedded human epidermis using anti- human Jak1 monoclonal antibody (Catalog # MAB4260). Tissue was stained using anti-rat HRP-DAB reagents (brown) and counterstained with hematox- ylin (blue).

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Human None Polyclonal Goat IgG AF1103 100 µg $355 JAM-A Mouse None Polyclonal Goat IgG AF1077 100 µg $355 JAM-C Mouse None Polyclonal Goat IgG AF1213 100 µg $355 JNK Pan Specific Human/Mouse/Rat None Polyclonal Rabbit IgG AF1387 100 µg $285 Phospho-JNK (T183/Y185) Human/Mouse/Rat None Polyclonal Rabbit IgG AF1205 50 µg $285

JNK1/JNK2 Human/Mouse/Rat None 252323 Mouse IgG2A MAB2076 100 µg $240

JNK1 Human/Mouse/Rat None 228601 Mouse IgG1 MAB17761 100 µg $240

JNK2 Human/Mouse/Rat None 252320 Mouse IgG2B MAB1846 100 µg $240

c-Jun aa 1-331 Human/Mouse None 320831 Mouse IgG1 MAB2670 100 µg $240 Kallikrein 3/PSA Human None Polyclonal Goat IgG AF1344 100 µg $355 Kallikrein 5 Human None Polyclonal Goat IgG AF1108 100 µg $355 Kallikrein 6/Neurosin Human None Polyclonal Goat IgG AF2008 100 µg $355

Kallikrein 6 in Human Breast Cancer. Kallikrein 6/ Neurosin was detected in paraffin-embedded human breast cancer tissue sections using anti-human Kalli- krein 6 affinity-purified polyclonal antibody (Catalog # AF2008). Tissues were stained using anti-goat HRP- DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Kallikrein 7 Human None Polyclonal Goat IgG AF2624 100 µg $355

Kallikrein 7 in Normal Human Skin. Kallikrein 7 (KLK7) was detected in paraffin-embedded normal human skin sections using anti-human KLK7 affinity- purified polyclonal antibody (Catalog # AF2624). Tis- sue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Kallikrein 9 Human None Polyclonal Goat IgG AF3486 100 µg $355

Kallikrein 9 in Human Ovarian Cancer. Kallikrein 9 (KLK9) was detected in paraffin-embedded human ovarian cancer sections using anti-human KLK9 affin- ity-purified polyclonal antibody (Catalog # AF3486). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

None Polyclonal Goat IgG AF1595 100 µg $355 Kallikrein 11 Human None 189308 Mouse IgG3 MAB1595 500 µg $325 Kallikrein 12 Human None Polyclonal Sheep IgG AF3095 100 µg $355 Kallikrein 13 Human None Polyclonal Goat IgG AF2625 100 µg $355

Kallikrein 13 in Human Prostate Cancer. Kallikrein 13 (KLK13) was detected in paraffin-embedded hu- man prostate cancer sections using anti-human KLK13 affinity-purified polyclonal antibody (Catalog # AF2625). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

70 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Kallikrein 15 Human None Polyclonal Goat IgG AF2540 100 µg $355 Human None Polyclonal Goat IgG AF3640 100 µg $355 KLF4 Mouse None Polyclonal Goat IgG AF3158 100 µg $355 KLF5 Human None Polyclonal Goat IgG AF3758 100 µg $355 None Polyclonal Goat IgG AF1819 100 µg $355 Klotho Mouse

None 236214 Rat IgG2A MAB1819 500 µg $325 Klotho in Mouse Kidney. Klotho was detected in a frozen section of mouse kidney tissue using anti- mouse Klotho affinity-purified polyclonal antibody (Catalog # AF1819). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Cata- log # CTS008; brown) and counterstained with hema- toxylin (blue).

None 387301 Mouse IgG1 MAB3895 100 µg $240 KOR Human

None 387310 Mouse IgG1 MAB38951 100 µg $240 Human None Polyclonal Goat IgG AF2127 100 µg $355 Kremen-1 Mouse None Polyclonal Goat IgG AF1647 100 µg $355 Kremen-1 Mouse Biotin Polyclonal Goat IgG BAF1647 50 µg $380

Laminin a1 Mouse None AL-4 Rat IgG2A MAB4656 100 µg $240 Laminin a1 in Mouse Decidua. Laminin a1 was detected in frozen sections of mouse E10.5 decidua using anti-mouse laminin a1 monoclonal antibody (Catalog # MAB4656; red). Tissue was counterstained with DAPI (blue).

Laminin a1 N-Terminus Domain VI Human None Polyclonal Goat IgG AF4187 100 µg $355 None Polyclonal Goat IgG AF3837 100 µg $355 Laminin a4 Mouse Biotin Polyclonal Goat IgG BAF3837 50 µg $380

Laminin g1 Human/Rat None D18 Mouse IgG2A MAB2139 100 µg $240

Laminin S Human/Rat/Chicken None C4 Mouse IgG1 MAB2066 100 µg $295

None AL-2 Rat IgG1 MAB2549 100 µg $240 Laminin-1 Mouse

None AL-3 Rat IgG1 MAB25491 100 µg $240

Laminin-5 Human None P3H9-2 Mouse IgG1 MAB2144 250 µg $240 Laminin-5 in Human MCF10A Cells. Laminin-5 was detected in human MCF10A breast epithelial cell line using anti-human Laminin-5 monoclonal antibody (Catalog # MAB2144). Cells were stained using NorthernLights-557 donkey anti-mouse IgG second- ary antibody (Catalog # NL007; red) and counter- stained with DAPI (blue).

LAMP1/CD107a Lumenal Domain Human None Polyclonal Sheep IgG AF4800 100 µg $355 Langerin/CD207 Human None Polyclonal Goat IgG AF2088 100 µg $355

Lck Human None 355713 Mouse IgG2B MAB3704 100 µg $240 Human None Polyclonal Goat IgG AF2148 100 µg $355 LDL R None Polyclonal Goat IgG AF2255 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF2255 50 µg $380

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE LEDGF Human None Polyclonal Goat IgG AF3468 100 µg $355 Human None Polyclonal Goat IgG AF398 100 µg $355 Leptin/OB Mouse None Polyclonal Goat IgG AF498 100 µg $355 None Polyclonal Goat IgG AF389 100 µg $355 Human

Leptin R None 52263 Mouse IgG2B MAB867 500 µg $325 Mouse None Polyclonal Goat IgG AF497 100 µg $355

Leptin Receptor in Rat Brain. Leptin Receptor was detected in cryostat tissue sections of rat brain using anti-mouse Leptin Receptor affinity-purified poly- clonal antibody (Catalog # AF497). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

None Polyclonal Goat IgG AF-250-NA 100 µg $355 Human

LIF None 9824 Mouse IgG2B MAB250 500 µg $325 Mouse None Polyclonal Total Goat IgG AB-449-NA 1 mg $355 LILRC1 Rat None Polyclonal Goat IgG AF4074 100 µg $355

LIM1 Human None 320416 Mouse IgG2A MAB2725 100 µg $240 None Polyclonal Goat IgG AF597 100 µg $355 Limitin Mouse Biotin Polyclonal Goat IgG BAF597 50 µg $380 LIN-28 Human None Polyclonal Goat IgG AF3757 100 µg $355 LIN-41 Human None Polyclonal Sheep IgG AF5104 100 µg $355 LINGO-1 Human None Polyclonal Goat IgG AF3086 100 µg $355

LINGO-1 aa 40-556 Human None 332237 Mouse IgG1 MAB30861 100 µg $240 None Polyclonal Goat IgG AF3679 100 µg $355 LINGO-2 Human

None 382007 Mouse IgG2A MAB36791 100 µg $240 None Polyclonal Goat IgG AF3885 100 µg $355 Lipin 1 Human Biotin Polyclonal Goat IgG BAF3885 50 µg $380 Lipin 3 Mouse None Polyclonal Goat IgG AF4064 100 µg $355 None Polyclonal Goat IgG AF1757 100 µg $355 Lipocalin-2/NGAL Human

None 220310 Rat IgG2A MAB1757 500 µg $325

LMIR3/CD300LF Human None 234903 Rat IgG2B MAB2774 100 µg $240 None Polyclonal Goat IgG AF2726 100 µg $355 LMO2 Human Biotin Polyclonal Goat IgG BAF2726 50 µg $380 LRRC4 Human None Polyclonal Sheep IgG AF4995 100 µg $355

LRRC4 in Human Cerebellum. Leucine rich repeat/ LRR-containing protein 4 (LRRC4) was detected in paraffin-embedded sections of human cerebellum using anti-human LRRC4 affinity-purified polyclonal antibody (Catalog # AF4995). Tissue was stained us- ing anti-sheep HRP-DAB secondary reagents (Catalog # CTS019; brown) and counterstained with hematox- ylin (blue).

72 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE LRRN1/NLRR-1 Human None Polyclonal Sheep IgG AF4990 100 µg $355

LRRN1 in Mouse Somites. Leucine rich repeat neu- ronal 1 (LRRN1) was detected in a frozen section from E9.5 mouse somite using anti-human LRRN1 affinity- purified polyclonal antibody (Catalog # AF4990). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

LRRTM2 Human/Mouse None Polyclonal Sheep IgG AF5589 100 µg $355 Human None Polyclonal Goat IgG AF2846 100 µg $355 Lumican Mouse None Polyclonal Goat IgG AF2745 100 µg $355

None PPZ0412 Mouse IgG2A PP-PPZ0412-00 100 µg $380 LXRa/NR1H3 Ligand Binding Domain Human

None PPZ0412 Mouse IgG2A PP-PPZ0412-10 100 µg $380 None Polyclonal Goat IgG AF1008 100 µg $355 Lymphotoxin bR/TNFRSF3 Mouse Biotin Polyclonal Goat IgG BAF1008 50 µg $380 Human/Mouse/Rat None Polyclonal Goat IgG AF3206 100 µg $355 Lyn aa 1-67

Human/Rat None 338716 Mouse IgG2B MAB3206 100 µg $240 None Polyclonal Goat IgG AF3428 100 µg $355 Lyp Human

None 340113 Mouse IgG2B MAB3428 100 µg $240 None Polyclonal Goat IgG AF2089 100 µg $355 Human Biotin Polyclonal Goat IgG BAF2089 50 µg $380 LYVE-1 None Polyclonal Goat IgG AF2125 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF2125 50 µg $380

None 223322 Rat IgG2A MAB2125 500 µg $325 MAdCAM-1 Mouse None Polyclonal Goat IgG AF993 100 µg $355 MafF Human None Polyclonal Goat IgG AF3917 100 µg $355 None Polyclonal Goat IgG AF538 100 µg $355 MAG/Siglec-4a Rat Biotin Polyclonal Goat IgG BAF538 50 µg $380

MAG/Siglec-4a in Rat Cerebellum. Myelin-associat- ed Glycoprotein (MAG/Siglec-4a) was detected in cryostat sections of rat cerebellum using anti-rat MAG affinity-purified polyclonal antibody (Catalog # AF538). Tissues were stained using the anti-goat HRP DAB Cell and Tissue Staining Kit (Catalog # CTS008); brown) and counterstained with hematoxylin (blue).

None Polyclonal Goat IgG AF2567 100 µg $355 Mash1 Mouse

None 290622 Rat IgG2A MAB2567 100 µg $240 Matrilin-2 Human None Polyclonal Goat IgG AF3044 100 µg $355 None Polyclonal Goat IgG AF932 100 µg $355 MCAM/CD146 Human Biotin Polyclonal Goat IgG BAF932 50 µg $380

Mcl-1 aa 1-230 Human None 542808 Mouse IgG2B MAB828 100 µg $240 M-CSF R Mouse None Polyclonal Sheep IgG AF3818 100 µg $355 Phospho-M-CSF R (Y723) Human None Polyclonal Rabbit IgG AF3268 50 µg $285 MD-1 Human None Polyclonal Goat IgG AF925 100 µg $355 MD-2 Human Biotin Polyclonal Sheep IgG BAF1787 50 µg $380 MDM2 Human/Mouse/Rat None Polyclonal Rabbit IgG AF1244 100 µg $285

MEK1/MEK2 Human/Mouse/Rat None 239416 Mouse IgG2B MAB2678 100 µg $240 Phospho-MEK1/MEK2 None Polyclonal Rabbit IgG AF2506 100 µg $285 (MEK1 S218/S2220, MEK2 S222/S226) Human/Mouse/Rat

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

MEK2 Human/Mouse/Rat None 300317 Mouse IgG2A MAB2855 100 µg $240 MELK Human None Polyclonal Sheep IgG AF4820 100 µg $355 None Polyclonal Goat IgG AF2740 100 µg $355 Melusin Human

None 308705 Mouse IgG2B MAB2740 100 µg $240 Meprin a Subunit/MEP1A Human None Polyclonal Goat IgG AF3220 100 µg $355 Human None Polyclonal Goat IgG AF2895 100 µg $355 Meprin b Subunit/MEP1B Mouse None Polyclonal Goat IgG AF3300 100 µg $355

Meprin b Subunit in Mouse Intestine. Meprin b Subunit (MEP1B) was detected in a frozen section of mouse intestine using anti-mouse MEP1B affinity- purified polyclonal antibody (Catalog # AF3300). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Human None Polyclonal Sheep IgG AF5577 100 µg $355 MESDC2 Mouse None Polyclonal Goat IgG AF4545 100 µg $355

MESDC2 in Adult Mouse Testis. Mesoderm devel- opment candidate 2 (MESDC2) was detected in section of mouse testis using anti-mouse MESDC2 affinity-purified polyclonal antibody Catalog # AF4545). The tissue was stained using anti-goat NorthernLight-557 secondary antibody (Catalog # NL001, red) and the nuclei were counterstained with DAPI (blue).

Mesothelin Isoform 1, aa 296-580 Human None 420404 Rat IgG2A MAB32651 100 µg $240 None Polyclonal Goat IgG AF3475 100 µg $355 Meteorin Mouse

None 347505 Rat IgG2B MAB3475 100 µg $240 Meteorin in Rat Cortical Stem Cells. Meteorin was detected in 7-day differentiated rat cortical stem cells using anti-mouse Meteorin monoclonal antibody (Catalog # MAB3475). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary anti- body (Catalog # NL013; red) and counterstained with DAPI (blue).

Human None 291204 Rat IgG1 MAB19151 100 µg $240 MFRP Mouse None Polyclonal Goat IgG AF3445 100 µg $355 mGluR1a C-Terminus Rat None Polyclonal Rabbit IgG PPS077 100 µL $355 mGluR3 Human None Polyclonal Sheep IgG AF4668 100 µg $355 None Polyclonal Sheep IgG AF4514 100 µg $355 mGluR5 Human

None 464823 Mouse IgG2B MAB45141 100 µg $240 mGluR5 in Human Brain (Putamen). Metabotropic glutamate receptor 5 (mGluR5) was detected in a paraffin-embedded human brain (putamen) section using anti-human mGluR5 affinity-purified poly- clonal antibody (Catalog # AF4514). The tissue was stained using anti-sheep HRP-DAB detection re- agents (brown) and counterstained with hematoxy- lin (blue).

74 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE mGluR5/1a Human/Mouse/Rat None Polyclonal Rabbit IgG PPS079 100 µL $355

mGluR8 Human None 476410 Mouse IgG2B MAB5277 100 µg $240 None Polyclonal Goat IgG AF2050 100 µg $355 MIA Human

None 294203 Mouse IgG2B MAB20501 100 µg $240 None Polyclonal Goat IgG AF1300 100 µg $355 MICA Human Biotin Polyclonal Goat IgG BAF1300 50 µg $380 Human None Polyclonal Goat IgG AF2946 100 µg $355 MICL/CLEC12A Mouse None Polyclonal Goat IgG AF2950 100 µg $355 Midkine Human None Polyclonal Goat IgG AF-258-PB 100 µg $355

Mineralocorticoid R/NR3C2 Human None H3122 Mouse IgG2A PP-H3122-00 100 µg $380

Mineralocorticoid R/NR3C2 aa 1-670 Human None 385707 Mouse IgG2B MAB4369 100 µg $240 MIS/AMH Rat None Polyclonal Goat IgG AF1446 100 µg $355 MIS/AMH Propeptide Human None Polyclonal Goat IgG AF2748 100 µg $355 Human None Polyclonal Sheep IgG AF4749 100 µg $355 MIS RII Rat None Polyclonal Goat IgG AF1618 100 µg $355 MITF Human None Polyclonal Goat IgG AF5769 100 µg $355

MIXL1 Human None 319919 Mouse IgG2A MAB2610 100 µg $240

MKK3 Human/Mouse/Rat None 275909 Mouse IgG2B MAB2515 100 µg $240

MKK4 Human None 323042 Mouse IgG2B MAB3390 100 µg $240 Phospho-MKK4 (S257/T261) Human/Mouse/Rat None Polyclonal Rabbit IgG AF2990 100 µg $285

MKK6 Human/Mouse/Rat None 304328 Rat IgG2B MAB1604 100 µg $240 None Polyclonal Goat IgG AF3579 100 µg $355 MKK7 Human

None 324302 Mouse IgG2B MAB3579 100 µg $240 MLK4a aa 560-570 Human/Mouse/Rat None Polyclonal Rabbit IgG AF3435 100 µg $285

Pan MLK-4 in Human Colon Cancer. Mixed lineage Kinase-4 (MLK-4) was detected in paraffin-embedded human colon cancer sections using anti-human/ mouse/rat pan MLK-4 affinity-purified polyclonal antibody (Catalog # AF3435). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti- rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxy- lin (blue).

MLX Human None Polyclonal Goat IgG AF4186 100 µg $355 None Polyclonal Goat IgG AF901 100 µg $355 MMP-1 Human

None 36665 Mouse IgG1 MAB901 200 µg $295

Pro MMP-1 Human None 36660 Mouse IgG1 MAB900 200 µg $295 None Polyclonal Goat IgG AF902 100 µg $355 Human

MMP-2 None 36006 Mouse IgG1 MAB902 200 µg $295 Mouse/Rat None Polyclonal Goat IgG AF1488 100 µg $355 None Polyclonal Goat IgG AF513 100 µg $355

MMP-3 Human None 50647 Mouse IgG1 MAB513 500 µg $325

None 10D6 Mouse IgG1 MAB905 200 µg $295 MMP-3 in Human Osteosarcoma Cell Line (MG- 63). Matrix Metalloproteinase-3 (MMP-3) was detect- ed in MG-63 cells using anti-human MMP-3 monoclo- nal antibody (Catalog # MAB513). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and coun- terstained with DAPI (blue).

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF907 100 µg $355 Human

MMP-7 None 111433 Mouse IgG2B MAB9071 500 µg $325 Mouse None Polyclonal Goat IgG AF2967 100 µg $355

Pro MMP-7 Human None 6A4 Mouse IgG1 MAB907 200 µg $295 None Polyclonal Goat IgG AF908 100 µg $355 MMP-8 Human Biotin Polyclonal Goat IgG BAF908 50 µg $380

None 100608 Mouse IgG2A MAB9081 500 µg $325 None Polyclonal Total Goat IgG AB911 1 mg $355 None Polyclonal Goat IgG AF911 100 µg $355 Human

None 4H3 Mouse IgG1 MAB911 200 µg $295 MMP-9

None 36020 Mouse IgG1 MAB936 500 µg $325 None Polyclonal Goat IgG AF909 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF909 50 µg $380 None Polyclonal Goat IgG AF910 100 µg $355

None 110304 Mouse IgG1 MAB910 500 µg $325 MMP-10 Human

None 117239 Mouse IgG2B MAB9101 500 µg $325

None 110316 Mouse IgG1 MAB9102 500 µg $325

MMP-12 Catalytic Domain Human None 82902 Mouse IgG2B MAB919 500 µg $325

MMP-12 Hemopexin Domain Human None 4D2 Mouse IgG1 MAB917 200 µg $295 None Polyclonal Goat IgG AF511 100 µg $355 MMP-13 Human

None 87512 Mouse IgG1 MAB511 500 µg $325 MMP-13 in Human Ovarian Cancer Tissue. Matrix metalloproteinase-13 (MMP-13) producing cells in human ovarian cancer tissue were detected using anti-human MMP-13 affinity-purified antibody (Cata- log # AF511). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and Hematoxylin (blue) counter- stain.

Pro MMP-13 Human None 87518 Mouse IgG2B MAB913 500 µg $325 None Polyclonal Goat IgG AF918 100 µg $355 MMP-14 Human

None 128527 Mouse IgG2B MAB9181 500 µg $325

MMP-14 aa 165-175 Human None 5H2 Mouse IgG1 MAB918 500 µg $325

MMP-15 Human None 130522 Mouse IgG1 MAB9161 500 µg $325

MMP-15 Catalytic Domain Human None 130516 Mouse IgG1 MAB916 500 µg $325 MMP-24/MT5-MMP Human/Mouse None Polyclonal Total Rabbit IgG AB924 200 µg $355

MMP-25/MT6-MMP Hemopexin Domain Human None 141825 Mouse IgG1 MAB11421 500 µg $325 None Polyclonal Goat IgG AF2534 100 µg $355 MMR Human

None 309210 Rat IgG2A MAB2534 100 µg $240 Human None Polyclonal Goat IgG AF2395 100 µg $355

Human/Mouse Biotin 296829 Rat IgG2B BAM2439 100 µg $380 MOG None Polyclonal Goat IgG AF2439 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF2439 50 µg $380

MRP1 Human None QCRL Mouse IgG1 MAB19291 100 µg $240

Human None 252608 Mouse IgG2B MAB2518 100 µg $240 MSK1 Human/Mouse None Polyclonal Goat IgG AF2518 100 µg $355 Phospho-MSK1 (S212) Human None Polyclonal Rabbit IgG AF1036 100 µg $285 Phospho-MSK2 (S196) Human/Mouse None Polyclonal Rabbit IgG AF189 100 µg $285 MUCDHL Human None Polyclonal Goat IgG AF3826 100 µg $355

76 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF2628 100 µg $355 Musashi-1 Human

None 282613 Mouse IgG2B MAB2628 100 µg $240 Musashi-1 in Human Intestine. Musashi-1 was de- tected in a paraffin-embedded human intestine sec- tion using anti-human Musashi-1 affinity-purified polyclonal antibody (Catalog # AF2628). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counter- stained with hematoxylin (blue).

Mxi1 Human None Polyclonal Goat IgG AF4185 100 µg $355 c-Myc aa 66-201 Human None Polyclonal Goat IgG AF3696 100 µg $355 None Polyclonal Goat IgG AF2928 100 µg $355 Human

None 316603 Rat IgG2A MAB2928 100 µg $240 MyD88

Mouse None 316902 Rat IgG2A MAB3109 100 µg $240 Mouse/Rat None Polyclonal Goat IgG AF3109 100 µg $355 Human None Polyclonal Goat IgG AF3174 100 µg $355 Myeloperoxidase/MPO Human/Mouse None Polyclonal Goat IgG AF3667 100 µg $355

Myeloperoxidase/MPO aa 279-745 Human/Mouse None 392105 Mouse IgG2B MAB3174 100 µg $240 Myeloperoxidase in Human Spleen. Myeloperoxi- dase (MPO) was detected in paraffin-embedded hu- man spleen tissue sections using anti-human/mouse MPO monoclonal antibody (Catalog # MAB3174) and Antigen Retrieval Reagent - Basic (Catalog # CTS013). The tissue was stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Myosin Heavy Chain Human None MF20 Mouse IgG2B MAB4470 100 µg $240 NAALADase-like 2/NAALADL2 Human None Polyclonal Goat IgG AF4665 100 µg $355 None Polyclonal Goat IgG AF1997 50 µg $355 Human Nanog Biotin Polyclonal Goat IgG BAF1997 50 µg $395 Mouse None Polyclonal Goat IgG AF2729 100 µg $355

Nanog in Human BG01V Embryonic Stem Cells. Nanog was detected in human BG01V embryonic stem cells using anti-human Nanog affinity-purified polyclonal antibody (Catalog # AF1997). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Nanog NorthernLights-conjugated Human NL493 Polyclonal Goat IgG NL1997G 500 µL $350

NCAM-1/CD56 Human None 301021 Mouse IgG2B MAB24081 100 µg $240 NCAM-L1 Human None Polyclonal Goat IgG AF277 100 µg $355 Nectin-3 Human None Polyclonal Goat IgG AF3064 100 µg $355 None Polyclonal Goat IgG AF2659 100 µg $355 Nectin-4 Human

None 337506 Mouse IgG2A MAB26591 100 µg $240

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE NEDD8 Human/Mouse/Rat None Polyclonal Sheep IgG AF4936 100 µg $355

NEDD8 in Human Skeletal Muscle. Neural precur- sor cell-expressed developmentally down-regulated gene 8 (NEDD8) was detected in paraffin-embedded human skeletal muscle sections using anti-human/ mouse/rat NEDD8 affinity-purified polyclonal anti- body (Catalog # AF4936). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Neogenin Mouse None Polyclonal Goat IgG AF1079 100 µg $355 Human None Polyclonal Sheep IgG AF4269 100 µg $355 Nephrin Mouse None Polyclonal Goat IgG AF3159 100 µg $355

Nephrin in Mouse Kidney. Nephrin was detected in frozen sections of mouse kidney using anti-mouse nephrin affinity-purified polyclonal antibody (Cata- log # AF3159). Tissue was stained using anti-goat IgG NorthernLights-557-conjugated secondary antibody (Catalog # NL001; red) and counterstained green.

None Polyclonal Goat IgG AF1182 100 µg $355 Human Biotin Polyclonal Goat IgG BAF1182 50 µg $380 Neprilysin/CD10

Human/Mouse None 200103 Rat IgG2B MAB1126 500 µg $325 Mouse None Polyclonal Goat IgG AF1126 100 µg $355

Human None 196908 Mouse IgG1 MAB1259 100 µg $240 Nestin None Polyclonal Goat IgG AF2736 100 µg $355 Rat

None 307501 Mouse IgG2A MAB2736 100 µg $240 Nestin in Undifferentiated Rat Neural Stem Cells. Nestin was detected in rat cortical stem cells using anti-rat Nestin monoclonal antibody (Catalog # MAB2736). The cells were stained using anti-mouse NorthernLights-557 secondary antibody (Catalog # NL007; red) and the nuclei counterstained with DAPI (blue).

Human NL637 196908 Mouse IgG1 NL1259V 500 µL $345 Nestin NorthernLights-conjugated

Rat NL557 307501 Mouse IgG2A NL2736R 500 µL $345 None Polyclonal Goat IgG AF1254 100 µg $355 Human Biotin Polyclonal Goat IgG BAF1254 50 µg $380 Netrin-4 None Polyclonal Goat IgG AF1132 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1132 50 µg $380

Netrin-4 in Mouse Brain. Netrin-4 was detected in frozen sections of mouse brain (cerebellum) using anti-mouse Netrin-4 affinity-purified polyclonal anti- body (Catalog # AF1132). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Netrin-G2a Mouse None 349724 Rat IgG2A MAB27441 100 µg $240

78 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE NRG1-a/HRG1-a EGF Domain Human None Polyclonal Goat IgG AF-296-NA 100 µg $355 NRG1-b1/HRG1-b1 EGF Domain Human None Polyclonal Goat IgG AF-396-NA 100 µg $355 Neuregulin-3/NRG3 EGF-like Domain Mouse None Polyclonal Goat IgG AF753 100 µg $355 Neurexin 3/NRXN3 Human/Mouse None Polyclonal Sheep IgG AF5269 100 µg $355 Neuritin Human None Polyclonal Goat IgG AF283 100 µg $355 Neurocan Mouse/Rat None Polyclonal Sheep IgG AF5800 100 µg $355 None Polyclonal Goat IgG AF2746 100 µg $355 NeuroD1 Human Biotin Polyclonal Goat IgG BAF2746 50 µg $380 NF-H Human None Polyclonal Goat IgG AF3108 100 µg $355

Neurofilament-H in Human Brain. Neurofilament-H (NF-H) was detected in paraffin-embedded human brain (cerebellum) tissue sections using anti-human NF-H affinity-purified polyclonal antibody (Catalog # AF3108). Tissues were stained using anti-goat HRP- DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

NF-L Human None 268014 Mouse IgG2B MAB2216 100 µg $240 NF-M Human None Polyclonal Sheep IgG AF3029 100 µg $355

Neurogenin-2 Human/Rat None 7G4 Mouse IgG2A MAB3314 100 µg $240 Human None Polyclonal Sheep IgG AF5685 100 µg $355 Neuroglycan C/CSPG5 Mouse/Rat None Polyclonal Goat IgG AF5665 100 µg $355

Neuroglycan C in Mouse Cerebellum. Neuroglycan C was detected in a section of adult mouse cerebel- lum using anti-mouse/rat Neuroglycan affinity-puri- fied polyclonal antibody (Catalog # AF5665). The cells were stained using NorthernLights-557 anti-goat lgG secondary antibody (Catalog # NL001; red) and the nuclei counterstained with DAPI (blue).

Neuronal Pentraxin R Human None Polyclonal Sheep IgG AF4414 100 µg $355 Neurophysin II Human None Polyclonal Goat IgG AF009 100 µg $355 Human None Polyclonal Sheep IgG AF3870 100 µg $355 Neuropilin-1/BDCA4 None Polyclonal Goat IgG AF566 100 µg $355 Rat

None 130604 Mouse IgG1 MAB5661 500 µg $325 Neuropilin-1 Expression in Mouse Embryonic Spi- nal Cord. Neuropilin-1 was detected in a cryostat section of mouse embryonic spinal cord (E15) using anti-rat Neuropilin-1 affinity-purified polyclonal anti- body (Catalog # AF566). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Cata- log # CTS008; brown) and counterstained with hema- toxylin (blue).

Neuropilin-2 Human None Polyclonal Goat IgG AF2215 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Neuroplastin Isoform 1 Human None Polyclonal Sheep IgG AF5174 100 µg $355

Neuroplastin in Human Cerebellum. Neuroplastin was detected in a paraffin-embedded human cere- bellum section using anti-human Neuroplastin affini- ty-purified polyclonal antibody (Catalog # AF5174). The tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Neuroplastin 65 Human/Mouse None Polyclonal Goat IgG AF5360 100 µg $355 None Polyclonal Goat IgG AF1235 100 µg $355 Neurotrimin Human Biotin Polyclonal Goat IgG BAF1235 50 µg $380 Human None Polyclonal Goat IgG AF387 100 µg $355 Neurturin Human/Mouse None Polyclonal Goat IgG AF477 100 µg $355 NF2/Merlin aa 363-578 Human None Polyclonal Goat IgG AF5616 100 µg $355

NFκB2 aa 1-447 Human None 291319 Mouse IgG1 MAB28881 100 µg $240

NG2/MCSP Human None LHM-2 Mouse IgG1 MAB2585 100 µg $240 Human None Polyclonal Goat IgG AF-256-NA 100 µg $355 b-NGF Rat None Polyclonal Goat IgG AF-556-NA 100 µg $355

Human None 74902 Mouse IgG1 MAB367 100 µg $240 NGF R/TNFRSF16 None Polyclonal Goat IgG AF1157 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1157 50 µg $380 Nicastrin Isoform 1 Human None Polyclonal Sheep IgG AF5378 100 µg $355 None Polyclonal Goat IgG AF2570 100 µg $355 Nidogen-1/Entactin Human Biotin Polyclonal Goat IgG BAF2570 50 µg $380

None 302117 Mouse IgG1 MAB2570 100 µg $240 None Polyclonal Goat IgG AF3385 100 µg $355 Nidogen-2 Human Biotin Polyclonal Goat IgG BAF3385 50 µg $380 Ninjurin-2 Human None Polyclonal Sheep IgG AF5056 100 µg $355

NKG2D/CD314 Mouse Biotin 191004 Rat IgG2B BAM1547 100 µg $380 None Polyclonal Goat IgG AF1850 100 µg $355 NKp46/NCR1 Human

None 195314 Mouse IgG2B MAB1850 500 µg $325 None Polyclonal Goat IgG AF2444 100 µg $355 NKX2.5 Human Biotin Polyclonal Goat IgG BAF2444 50 µg $380

None 259416 Mouse IgG1 MAB2444 100 µg $240 NKX6.1 Human/Mouse None Polyclonal Goat IgG AF5857 100 µg $355 NMDA R, NR1 Subunit C1 Splice Variant Mouse/Rat None Polyclonal Rabbit IgG PPS080 25 µg $355 Human/Mouse/Rat None Polyclonal Rabbit IgG PPS082 25 µg $355 NMDA R, NR1 Subunit C2 Splice Variant Mouse/Rat None Polyclonal Rabbit IgG PPS081 25 µg $355 NMDA R, NR1 Subunit N1 Splice Variant Mouse/Rat None Polyclonal Rabbit IgG PPS083 25 µg $355 NMDA R, NR2A Subunit Human/Mouse/Rat None Polyclonal Rabbit IgG PPS012 10 µg $355 NMDA R, NR2B Subunit Human/Mouse/Rat None Polyclonal Rabbit IgG PPS013 10 µg $355 NMDA R, NR2C Subunit Human/Mouse/Rat None Polyclonal Rabbit IgG PPS033 10 µg $355

N-Me-6,7-diOH-TIQ Multispecies None 106814 Mouse IgG2B MAB167 500 µg $325 Noggin Mouse None Polyclonal Goat IgG AF719 100 µg $355 Nogo-A aa 2-172 Rat None Polyclonal Goat IgG AF3098 100 µg $355

80 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Nogo-A aa 566-748 Human None Polyclonal Sheep IgG AF3515 100 µg $355

Nogo-A in differentiated rat cortical stem cells. Nogo-A was detected in 7-day differentiated rat cor- tical stem cells using anti-human Nogo-A affinity- purified polyclonal antibody (Catalog # AF3515). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

Nogo-C Human None 499825 Mouse IgG3 MAB5766 100 µg $240 Norrin Human None Polyclonal Goat IgG AF3014 100 µg $355 None Polyclonal Goat IgG AF950 100 µg $355 eNOS aa 1030-1203 Human Biotin Polyclonal Goat IgG BAF950 50 µg $380

iNOS aa 781-798 Human None 2D2-B2 Mouse IgG1 MAB9502 100 µg $240 nNOS Human None Polyclonal Goat IgG AF2416 100 µg $355 Notch-1 Rat None Polyclonal Goat IgG AF1057 100 µg $355

None 433802 Mouse IgG2A MAB3647 100 µg $240 Notch-1 Intracellular Domain, aa 2428-2556 Human None Polyclonal Sheep IgG AF3647 100 µg $355

Notch-1 in Human SAOS-2 Cells. Notch-1 was de- tected in human SAOS-2 osteosarcoma cells using anti-human Notch-1 affinity-purified polyclonal anti- body (Catalog # AF3647). Cells were stained using NorthernLights-557 donkey anti-sheep IgG second- ary antibody (Catalog # NL010; red) and counter- stained with DAPI (blue).

Notch-2 Rat None Polyclonal Goat IgG AF1190 100 µg $355

Notch-2 in Rat Brain. Notch-2 was detected in cryo- stat sections of rat brain (choroid plexus) using anti-rat Notch-2 affinity-purified polyclonal antibody (Catalog # AF1190). Tissues were stained using anti- goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

Notch-2 Intracellular Domain Human None Polyclonal Goat IgG AF3735 100 µg $355 None Polyclonal Goat IgG AF1308 100 µg $355 Notch-3 Mouse Biotin Polyclonal Goat IgG BAF1308 50 µg $380 None Polyclonal Goat IgG AF3847 100 µg $355 Notch-4 Intracellular Domain Human

None 411913 Rat IgG2A MAB3847 100 µg $240 None Polyclonal Goat IgG AF1640 100 µg $355 NOV/CCN3 Human Biotin Polyclonal Goat IgG BAF1640 50 µg $380

NRAGE Human None 353124 Mouse IgG2B MAB3835 100 µg $240 NrCAM Isoform 3 Human None Polyclonal Goat IgG AF2034 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE NT-3 Human None Polyclonal Goat IgG AF-267-NA 100 µg $355

NT-3 in Rat Cerebellum. Neurotrophin-3 (NT-3) was detected in a cryostat section of rat cerebellar Purkinje neurons using anti-human NT-3 affinity- purified polyclonal antibody (Catalog # AF-267-NA). Tissues were stained with AEC substrate (red) and counterstained with hematoxylin (blue).

NT-4 Human None Polyclonal Goat IgG AF-268-NA 100 µg $355 Nucleostemin Human/Mouse/Rat None Polyclonal Goat IgG AF1638 100 µg $355

Nucleostemin in Human U2OS Cells. Nucleostemin was detected in the nucleoli of the U2OS human osteosarcoma cell line using anti-human/mouse/ rat Nucleostemin affinity-purified polyclonal anti- body (Catalog # AF1638). Cells were stained using NorthernLights-557 anti-goat IgG secondary anti- body (Catalog # NL001; red) and counterstained with DAPI (blue).

None Polyclonal Sheep IgG AF4338 100 µg $355 Numb Human Biotin Polyclonal Sheep IgG BAF4338 50 µg $380

None 447716 Mouse IgG2A MAB4338 100 µg $240

Human None N1404 Mouse IgG2A PP-N1404-00 100 µg $380 Nurr-1/NR4A2 Human/Mouse None Polyclonal Goat IgG AF2156 100 µg $355 N-WASP Human None Polyclonal Goat IgG AF3854 100 µg $355 OAS2 Human None HLS56/3 Mouse N/A MAB1925 100 µg $240 OBCAM Human None Polyclonal Goat IgG AF2777 100 µg $355 None Polyclonal Goat IgG AF1759 100 µg $355 Human Oct-3/4 Biotin Polyclonal Goat IgG BAF1759 50 µg $380

Human/Mouse None 240408 Rat IgG2B MAB1759 100 µg $240 Oct-3/4 and Alkaline Phosphatase in Human BG01V Cells. Oct-3/4 was detected in BG01V embryonic stem cells using anti-human Oct-3/4 affinity-purified polyclonal antibody (Catalog # AF1759). Alkaline phosphatase (ALPL) was detected using anti-human/mouse/rat ALPL monoclonal­ antibody (Catalog # MAB1448). Cells were stained for Oct-3/4 using NorthernLights-637 don­key anti- goat IgG secondary antibody (Catalog # NL002; red) and stained for ALPL using NorthernLights-557 donkey anti-mouse IgG secondary antibody (pseudo-stained green; Catalog # NL007).

Oct-3/4 NorthernLights-conjugated Human/Mouse NL493 240408 Rat IgG2B NL1759G 500 µL $345 None Polyclonal Goat IgG AF2417 100 µg $355 Olig1 Human

None 257219 Mouse IgG2B MAB2417 100 µg $240

Olig 1, 2, 3 Human None 257224 Mouse IgG2A MAB2230 100 µg $295

82 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF2418 100 µg $355 Olig2 Human Biotin Polyclonal Goat IgG BAF2418 50 µg $380

Olig2 and Oligodendrocyte Marker O4 in Rat Cor- tical Stem Cells (RCSC). Olig2 and O4 were detected in 7 day differentiated RCSC using anti-human Olig2 affinity-purified polyclonal antibody (Catalog # AF2418) and anti-human/mouse/rat/chicken O4 monoclonal antibody (Catalog # MAB1326). Cells were stained for Olig2 using NorthernLights-637 an- ti-goat IgG secondary antibody (Catalog # NL002; red) and for O4 using an anti-mouse IgM Secondary antibody (green).

None Polyclonal Goat IgG AF2356 100 µg $355 Human Olig3 Biotin Polyclonal Goat IgG BAF2356 50 µg $380

Human/Mouse None 257934 Mouse IgG1 MAB2456 100 µg $240 Oligodendrocyte Marker O1 Human/Mouse/Rat/Chicken None O1 Mouse IgM MAB1327 50 µg $200 Oligodendrocyte Marker O4 Human/Mouse/Rat/Chicken None O4 Mouse IgM MAB1326 50 µg $2040

Oligodendrocyte Marker O4 in Rat Cortical Stem Cells (RCSC). Oligodendrocyte marker O4 (O4) was detected in 7-day differentiated RCSC using anti-hu- man/mouse/rat/chicken O4 monoclonal antibody (Catalog # MAB1326). Cells were stained using NorthernLights-557 donkey anti-mouse IgG second- ary antibody (Catalog # NL007; red) and counter- stained with DAPI (blue).

Oligodendrocyte Marker O4 NorthernLights-conjugated Human/Mouse/Rat/Chicken NL637 O4 Mouse IgM NL1326V 500 µL $345

Biotin 97505 Mouse IgG1 BAM763 250 µg $380 Orexin A Human/Mouse/Rat

None 97505 Mouse IgG1 MAB763 500 µg $325

Orexin B Human None 145202 Mouse IgG1 MAB734 500 µg $325 Orexin B, p38a, & Integrin b1 in Mouse Brain Stem. Orexin B, p38a, and Integrin b 1 were detected in frozen sections of mouse brain stem using anti- human Orexin B monoclonal antibody (Catalog # MAB734), anti-human/mouse/rat p38a affinity-puri- fied polyclonal antibody (Catalog # AF8691), and anti-mouse Integrin b1 affinity-purified polyclonal antibody (Catalog # AF2405). Tissue was stained with NorthernLights (NL) secondary polyclonal antibod- ies: NL-493 anti-rabbit IgG (Catalog # NL006; green), NL-557 anti-mouse IgG (Catalog # NL007; red), and NL-637 anti-goat IgG (Catalog # NL002; blue).

Oncostatin M/OSM Mouse None Polyclonal Goat IgG AF-495-NA 100 µg $355

Human None 469221 Mouse IgG1 MAB4389 100 µg $240 None Polyclonal Goat IgG AF662 100 µg $355 OSM Rb

Mouse Biotin 118125 Rat IgG2A BAM662 100 µg $240

None 118125 Rat IgG2A MAB662 100 µg $240

Osteoactivin/GPNMB Human None 303822 Mouse IgG2B MAB25501 100 µg $240

Osteocalcin Human None 190125 Mouse IgG1 MAB1419 100 µg $240 None Polyclonal Goat IgG AF1433 100 µg $355 Human Biotin Polyclonal Goat IgG BAF1433 50 µg $380 Osteopontin/OPN

None 190312 Mouse IgG2B MAB1433 100 µg $240 Mouse None Polyclonal Goat IgG AF808 100 µg $355 None Polyclonal Goat IgG AF805 100 µg $355 Human Osteoprotegerin/TNFRSF11B Biotin Polyclonal Goat IgG BAF805 50 µg $380 Mouse Biotin Polyclonal Goat IgG BAF459 50 µg $380

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF1979 100 µg $355 Otx2 Human Biotin Polyclonal Goat IgG BAF1979 50 µg $380

None 246826 Mouse IgG2B MAB1979 100 µg $240

OV-6 Human/Rat None OV-6 Mouse IgG1 MAB2020 100 µg $295

Human None 159403 Mouse IgG1 MAB10541 500 µg $325 OX40 Ligand/TNFSF4 Mouse None Polyclonal Goat IgG AF1236 100 µg $355 None Polyclonal Goat IgG AF1047 100 µg $355 p21/CIP1/CDKN1A Human Biotin Polyclonal Goat IgG BAF1047 50 µg $380

None 195720 Mouse IgG2A MAB1047 200 µg $240 p21 in Human Breast Cancer. p21 was detected in paraffin-embedded human breast cancer tissue sec- tions using anti-human p21 affinity-purified poly- clonal antibody (Catalog # AF1047). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counter- stained with haematoxylin (blue).

Human None Polyclonal CTS2256 50 Tests $425 p27/Kip1

Human/Mouse/Rat None 341116 Rat IgG2A MAB22561 100 µg $240 Phospho-p38 MAP Kinase (T180/Y182) Human/Mouse/Rat None Polyclonal Rabbit IgG AF869 100 µg $285

Phosphorylated p38 in Embryonic Mouse Eye. Phosphorylated p38 was detected in a frozen section of E15 mouse eye cup using anti-phospho-p38 (T180/Y182) affinity-purified polyclonal antibody (Catalog # AF869). The tissue was stained using anti- rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxy- lin (blue).

None Polyclonal Rabbit IgG AF8691 100 µg $285 p38a Human/Mouse/Rat

None 142102 Mouse IgG2B MAB869 100 µg $240

p38b Human None 280071 Mouse IgG2B MAB3274 100 µg $240 p38g Human/Mouse/Rat None Polyclonal Rabbit IgG AF1347 100 µg $285 p38d Human None Polyclonal Rabbit IgG AF1519 100 µg $285 None Polyclonal Rabbit IgG AF1043 100 µg $285 Phospho-p53 (S15) Human

None 261366 Mouse IgG1 MAB18391 100 µg $240 Phospho-p53 (S46) Human None Polyclonal Rabbit IgG AF1489 100 µg $285 None Polyclonal Goat IgG AF1916 100 µg $355 p63/TP73L Human Biotin Polyclonal Goat IgG BAF1916 50 µg $380

None 241631 Mouse IgG2B MAB1916 100 µg $240 None Polyclonal Rabbit IgG AF8962 100 µg $285 p70 S6 Kinase Human/Mouse/Rat None Polyclonal Goat IgG AF8964 100 µg $355

None 215247 Mouse IgG1 MAB8962 100 µg $240 Phospho-p70 S6 Kinase (T229) Human None Polyclonal Goat IgG AF896 100 µg $285

84 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

PAK7 Human/Rat None 444703 Mouse IgG2B MAB4696 100 µg $240 PAK7 in Human Cerebellum. p21-activated kinase 7 (PAK7) was detected in a paraffin-embedded human cerebellum section using anti-human PAK7 monoclo- nal antibody (Catalog # MAB4696). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Stain- ing Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Park7/DJ-1 Human None Polyclonal Goat IgG AF3995 100 µg $355 Parkin Human None Polyclonal Goat IgG AF1438 100 µg $355

PARP Multi-species None C2-10 Mouse IgG1 4338-MC-50 50 µg $195 Parvalbumin a Human/Mouse/Rat None Polyclonal Sheep IgG AF5058 100 µg $355

Parvalbumin a in Human Cortex. Parvalbumin a was detected in paraffin-embedded sections of hu- man cortex using anti-human/mouse/rat Parvalbu- min a monoclonal antibody (Catalog # AF5058). Tis- sue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Patched (First Extracellular Loop) Mouse None 413220 Rat IgG2A MAB41051 100 µg $240 Patched in Embryonic Mouse Spinal Cord. Patched was detected in ventral E13.5 mouse spinal cord using anti-mouse Patched monoclonal antibody (Catalog # MAB41051; red). Tissue was counter- stained with DAPI (blue).

Patched 2 (First Extracellular Loop) Human None Polyclonal Goat IgG AF4078 100 µg $355 Human None Polyclonal Goat IgG AF2457 100 µg $355 Pax3

Human/Mouse None 274212 Mouse IgG2A MAB2457 100 µg $240 Pax4 Human None Polyclonal Goat IgG AF2614 100 µg $355 Pax5 Human None Polyclonal Goat IgG AF3487 100 µg $355

Biotin PAX6 Mouse IgG1 BAM1260 100 µg $260 Pax6 Mouse/Rat/Chicken

None PAX6 Mouse IgG1 MAB1260 100 µg $240

Pax7 Human/Mouse/Rat/Chicken None PAX7 Mouse IgG1 MAB1675 100 µg $240 Human/Mouse/Rat None Polyclonal Rabbit IgG 6361-PC-100 100 µL $310 PBR Mouse/Rat None Polyclonal Rabbit IgG 6362-PC-100 100 µL $310 Human None Polyclonal Goat IgG AF1086 100 µg $355 PD-1 None Polyclonal Goat IgG AF1021 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1021 50 µg $380 None Polyclonal Goat IgG AF1143 100 µg $355 PD-ECGF Human Biotin Polyclonal Goat IgG BAF1143 50 µg $380 None Polyclonal Total Goat IgG AB-221-NA 1 mg $355 Human PDGF-AA None Polyclonal Goat IgG AF-221-NA 100 µg $355 Rat None Polyclonal Goat IgG AF1055 100 µg $355 Human None Polyclonal Goat IgG AF1560 100 µg $355 PDGF-C Mouse None Polyclonal Goat IgG AF1447 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE PDGF-D Human None Polyclonal Goat IgG AF1159 100 µg $355 None Polyclonal Goat IgG AF-307-NA 100 µg $355 Human

None 35248 Mouse IgG1 MAB322 500 µg $325 PDGF Ra None Polyclonal Goat IgG AF1062 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1062 50 µg $380 Phospho-PDGF Ra (Y742) Human None Polyclonal Rabbit IgG AF2114 50 µg $285 Phospho-PDGF Ra (Y762) Human None Polyclonal Rabbit IgG AF21141 50 µg $285 None Polyclonal Goat IgG AF385 100 µg $355 Human

None PR7212 Mouse IgG1 MAB1263 250 µg $230 PDGF Rb None Polyclonal Goat IgG AF1042 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1042 50 µg $380

PDGF Rb in Mouse Embryo. Platelet-derived Growth Factor Receptor b (PDGF Rb) was detected in a cryo- stat tissue section of E13 mouse spinal cord using anti-mouse PDGF Rb affinity-purified polyclonal anti- body (Catalog # AF1042). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

None Polyclonal Goat IgG AF1224 100 µg $355 Human

None 176611 Mouse IgG2B MAB1224 500 µg $325 PD-L2 None Polyclonal Goat IgG AF1022 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1022 50 µg $380 None Polyclonal Goat IgG AF2419 100 µg $355 Human PDX-1/IPF1 Biotin Polyclonal Goat IgG BAF2419 50 µg $380

Human/Mouse None 267712 Mouse IgG2B MAB2419 100 µg $240 PDZK1 Human/Mouse None Polyclonal Sheep IgG AF4997 100 µg $355 PEDF R Human/Mouse/Rat None Polyclonal Sheep IgG AF5365 100 µg $355 PER1 aa 159-365 Human None Polyclonal Goat IgG AF4565 100 µg $355

Periostin/OSF-2 Mouse None 345613 Rat IgG2A MAB3548 100 µg $240 Periostin/OSF-2 Isoform 2 Mouse None Polyclonal Goat IgG AF2955 100 µg $355

Persephin Human None 275508 Mouse IgG2B MAB2388 100 µg $240 PIBF1 Human None Polyclonal Sheep IgG AF5559 100 µg $355 pIgR Mouse None Polyclonal Goat IgG AF2800 100 µg $355 PIN Human None Polyclonal Goat IgG AF877 100 µg $355

Pin1 aa 2-163 Human/Mouse None 257417 Mouse IgG2B MAB2294 100 µg $240

PINK1 Human None 420920 Rat IgG2A MAB4357 100 µg $240 PKCb1 Human/Rat None Polyclonal Goat IgG AF3759 100 µg $355 PKCι/λ PKC ι/λ/ζ Cross-reactive, aa 446-587 Human/Mouse/Rat None Polyclonal Sheep IgG AF4465 100 µg $355

PKR Human None HL71/10 Mouse IgG1 MAB1980 100 µg $295 Plasminogen Kringle 5 Mouse None Polyclonal Goat IgG AF742 100 µg $355

PLC-g2 aa 400-600 Human/Mouse None 346404 Mouse IgG1 MAB3716 100 µg $240

Plexin A1 Mouse None 408305 Rat IgG2A MAB4309 100 µg $240

86 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Plexin A3 Mouse None Polyclonal Goat IgG AF4075 100 µg $355

Plexin A3 Expression in Embryonic Spinal Cord. Plexin A3 was detected in mouse embryonic (E13.5) spinal cord using anti-mouse Plexin A3 affinity-puri- fied polyclonal antibody (Catalog # AF4075). Cells were stained using a NorthernLights-557 secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Plexin B1 Human None Polyclonal Goat IgG AF3749 100 µg $355 None Polyclonal Sheep IgG AF5329 100 µg $355 Plexin B2 Human

None 537237 Mouse IgG2A MAB5329 100 µg $240 Plexin B2 in Embryonic Mouse Choroid Plexus. Plexin B2 in Human Colon Cancer. Plexin B2 was Plexin B2 was detected in a section of embryonic detected in paraffin-embedded human colon cancer (E13.5) mouse choroid plexus using anti-human sections using anti-human Plexin B2 affinity-purified Plexin B2 affinity-purified polyclonal antibody (Cata- polyclonal antibody (Catalog # AF5329). The tissue log # AF5329). The tissue was stained using anti- was subjected to antigen retrieval using the Basic sheep NorthernLights-557 secondary antibody (Cat- Antigen Retrieval Kit (Catalog # CTS013) and stained alog # NL010; red) and the nuclei counterstained with using the anti-sheep HRP-DAB Cell and Tissue Stain- DAPI (blue). ing Kit (Catalog # CTS019; brown). The tissue was counterstained with hematoxylin (blue).

PNR/NR2E3 Human None H7223 Mouse IgG2A PP-H7223-00 100 µg $380 None Polyclonal Goat IgG AF1658 100 µg $355 Human Biotin Polyclonal Goat IgG BAF1658 50 µg $380

None 222328 Mouse IgG2A MAB1658 100 µg $240 Podocalyxin None Polyclonal Goat IgG AF1556 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1556 50 µg $380

None 192703 Rat IgG2B MAB1556 100 µg $240 Podocalyxin in Human Kidney. Podocalyxin was de- Podocalyxin in Human Embryonic Stem Cells tected in a paraffin-embedded human kidney tissue (BGO1V). Podocalyxin was detected in the BGO1V section using anti-human Podocalyxin affinity-puri- human embryonic stem cell line cultured on mouse fied polyclonal antibody (Catalog # AF1658). Tissues embryonic fibroblasts using anti-human Podocalyxin were stained using the anti-goat HRP-DAB Cell and monoclonal antibody (Catalog # MAB1658). The cells Tissue Staining Kit (Catalog # CTS008; brown) and were stained with anti-mouse NorthernLights-557 counterstained with hematoxylin (blue). secondary antibody (Catalog # NL007; red) and coun- terstained with DAPI (blue).

Podocalyxin NorthernLights-conjugated Human NL557 222328 Mouse IgG2A NL1658R 500 µL $345

Human None 390117 Mouse IgG2B MAB42201 100 µg $240 Podocan Mouse None Polyclonal Goat IgG AF3104 100 µg $355 Human None Polyclonal Sheep IgG AF3670 100 µg $355 Podoplanin Mouse None Polyclonal Goat IgG AF3244 100 µg $355 Potassium Channel Kv2.2 Rat/Xenopus None Polyclonal Rabbit IgG PPS015 100 µL $355 Phospho-Potassium Channel Kv3.1 (S503) Mouse/Rat None Polyclonal Rabbit IgG PPS016 100 µL $355

PP2A Catalytic Subunit, aa 209-309 Human None 234532 Rat IgG2B MAB16531 100 µg $240

PP2Cg/PPM1G Human None 528322 Mouse IgG1 MAB5595 100 µg $240

PPARg/NR1C3 Human None A3409A Mouse IgG2A PP-A3409A-00 100 µg $380 None Polyclonal Goat IgG AF166 100 µg $355 Presenilin-1 C-Terminal Fragment, aa 298-407 Human Biotin Polyclonal Goat IgG BAF166 50 µg $380 None Polyclonal Goat IgG AF149 100 µg $355 Presenilin-1 N-Terminal Fragment, aa 1- 80 Human

None 121121 Mouse IgG2B MAB149 500 µg $325 Presenilin-2 C-Terminal Fragment, aa 322-388 Human Biotin Polyclonal Goat IgG BAF197 50 µg $380

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Presenilin-2 N-Terminal Fragment, aa 1- 80 Human None Polyclonal Goat IgG AF153 100 µg $355

PRL/PTP4A Pan Specific Human None 334407 Mouse IgG2B MAB32191 100 µg $240

None A9621A Mouse IgG2A PP-A9621A-00 100 µg $380 Human

Progesterone R/NR3C3 None 1154 Mouse IgG1 PPS017 100 µL $355

Human/Mouse/Rat/Primate None 608 Mouse IgG1 PPS018 100 µL $355

Progesterone R/NR3C3 B Human None H5344 Mouse IgG2A PP-H5344-00 100 µg $380 Progesterone Receptor-B/NR3C3 in Human Endo- metrium. Progesterone Receptor isoform B was de- tected in paraffin-embedded human endometrial epithelial cells using anti-human Progesterone Re- ceptor isoform B monoclonal antibody (Catalog # PP- H5344-00). Tissues were stained with a 0.01% DAB solution (brown) and counterstained with hematoxy- lin (blue).

Human None Polyclonal Goat IgG AF2420 100 µg $355 Progranulin None Polyclonal Sheep IgG AF2557 100 µg $355 Mouse

None 333731 Rat IgG2A MAB25571 100 µg $240 Prohibitin Human None Polyclonal Goat IgG AF3470 100 µg $355 None Polyclonal Goat IgG AF1445 100 µg $355 Prolactin Mouse Biotin Polyclonal Goat IgG BAF1445 50 µg $380 Human None Polyclonal Goat IgG AF1167 100 µg $355 Prolactin R Rat None Polyclonal Goat IgG AF1112 100 µg $355

Prominin 2 Human None 244029 Mouse IgG2B MAB2024 100 µg $295 Prostasin/Prss8 Human None Polyclonal Sheep IgG AF4599 100 µg $355

Prostasin in Human Prostate Cancer. Prostasin was detected in a paraffin-embedded human prostate cancer section using anti-human Prostasin affinity-purified polyclonal antibody (Catalog # AF4599). The tissue was subjected to antigen retriev- al using the Basic Antigen Retrieval Kit (Catalog # CTS013). The section was stained using the anti- sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxy- lin (blue).

Protocadherin a1 Human None Polyclonal Sheep IgG AF5064 100 µg $355

Protocadherin a1 in Mouse Cerebellum. Proto­ cadherin a1 was detected in a section of adult mouse cerebellum using anti-mouse affinity-purified poly- clonal antibody (Catalog # AF5064). The tissue was stained using anti-sheep NorthernLights-557 sec- ondary antibody (Catalog # NL010; red) and the nu- clei counterstained with DAPI (blue).

Protocadherin-1 Human None Polyclonal Goat IgG AF5899 100 µg $355

88 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Protogenin Mouse None Polyclonal Sheep IgG AF4919 100 µg $355

Protogenin in Embryonic Neural Tube. Protogenin (PRTG) was detected in a section of E9 mouse neural tube using anti-mouse PRTG affinity-purified poly- clonal antibody (Catalog # AF4919). The tissue was stained using NorthernLights-557 anti-sheep poly- clonal antibody (Catalog # NL101; red) and counter- stained with DAPI (blue).

Prox1 Human None Polyclonal Goat IgG AF2727 100 µg $355 PSMA/FOLH1/NAALADase I Human None Polyclonal Sheep IgG AF4234 100 µg $355

PTEN Human/Mouse/Rat None 217702 Mouse IgG1 MAB847 100 µg $240 PTEN aa 385-403 Human/Mouse/Rat None Polyclonal Rabbit IgG AF847 100 µg $285 PTH1 R Human None Polyclonal Sheep IgG AF5709 100 µg $355 PTP1B Human None Polyclonal Rabbit IgG AF1366 100 µg $285

PTP b/ζ aa 302-754 Human None 279245 Rat IgG2A MAB26881 100 µg $240

PTP-MEG2 Human/Mouse/Rat None 291835 Mouse IgG2B MAB2668 100 µg $240 PTPRg aa 111-541 Human None Polyclonal Goat IgG AF4608 100 µg $355

PTPRg in Human Kidney. Protein Tyrosine Phospha- tase, Receptor type g (PTPRg) was detected in paraf- fin-embedded normal human kidney tissue sections using anti-human PTPRg affinity-purified polyclonal antibody (Catalog # AF4608). Tissues were stained us- ing anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

PTPRσ aa 30-1260 Human None Polyclonal Goat IgG AF3430 100 µg $355 RACK1 Human/Mouse/Rat None Polyclonal Goat IgG AF3434 100 µg $355 Rae-1g Mouse None Polyclonal Goat IgG AF1136 100 µg $355 Human None Polyclonal Goat IgG AF1145 100 µg $355 RAGE Mouse None Polyclonal Goat IgG AF1179 100 µg $355

Mouse/Rat None 175410 Rat IgG2A MAB1179 500 µg $325 RAGE Expression in Human Alzheimer’s Brain. Re- ceptor for Advanced Glycation End products (RAGE) was detected in paraffin-embedded Alzheimer’s dis- ease human cerebellum sections using anti-human RAGE affinity-purified polyclonal antibody (Catalog # AF1145). Tissue was stained using the anti-goat HRP- DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Human None 80707 Mouse IgG2A MAB6831 500 µg $325 RANK/TNFRSF11A Mouse None Polyclonal Goat IgG AF692 100 µg $355 Rap1A/B Human/Mouse/Rat None Polyclonal Goat IgG AF3767 100 µg $355 RARRES1 Mouse None Polyclonal Goat IgG AF4657 100 µg $355 Reelin Mouse None Polyclonal Goat IgG AF3820 100 µg $355 Reg II Mouse None Polyclonal Goat IgG AF2035 100 µg $355

Reg 2/PAP Rat None 293523 Mouse IgG1 MAB1996 100 µg $240

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Reg IIIb Mouse None Polyclonal Sheep IgG AF5110 100 µg $355

Reg IIIb in Mouse Small Intestine. Reg IIIb was de- tected in adult mouse small intestine sections using anti-mouse Reg IIIb affinity-purified polyclonal anti- body (Catalog # AF5110). Cells were stained using NorthernLights-557 donkey anti-sheep IgG second- ary antibody (Catalog # NL010; red) and counter- stained with DAPI (blue).

Reg IIId Mouse None 518818 Rat IgG2A MAB5678 100 µg $240 Reg IV Human None Polyclonal Goat IgG AF1379 100 µg $355

RelB aa 382-579 Human None 315206 Mouse IgG2B MAB2698 100 µg $240 RelB in Human Lymphoma. RelB was detected in a paraffin-embedded human lymphoma section using anti-human RelB monoclonal antibody (Catalog # MAB2698). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxy- lin (blue).

c-Rel Human None 321422 Rat IgG2A MAB4606 50 µg $240 Human/Mouse None Polyclonal Goat IgG AF2699 100 µg $355 c-Rel aa 1-588

Mouse Biotin 290512 Rat IgG2A BAM2699 100 µg $380 c-Rel in Mouse Splenocytes. c-Rel was detected in mouse splenocytes using anti-mouse c-Rel biotinyl- ated monoclonal antibody (Catalog # BAM2699).Cells were stained using NorthernLights Streptavidin-557 (Catalog # NL999; red) and counterstained with DAPI (blue)..

Renin Human None Polyclonal Sheep IgG AF4090 100 µg $355 Renin 1 Mouse None Polyclonal Goat IgG AF4277 100 µg $355

Renin 1 Propeptide Mouse None 434703 Rat IgG2A MAB42771 100 µg $240

Renin Propeptide Human None 411507 Mouse IgG1 MAB4447 100 µg $240 Resistin Mouse None Polyclonal Goat IgG AF1069 100 µg $355 Human None Polyclonal Goat IgG AF1485 100 µg $355 Ret Mouse None Polyclonal Goat IgG AF482 100 µg $355 REVERBb/NR1D2 Human None H2729 Mouse IgG3 PP-H2729-00 100 µg $380 None Polyclonal Sheep IgG AF3598 100 µg $355 Rex-1 Human Biotin Polyclonal Sheep IgG BAF3598 50 µg $380

None 373722 Rat IgG2A MAB3598 100 µg $240 Rex-1 in Human NTera2 Cells. Rex-1 was detected in human NTera2 embryonic carcinoma cell line using anti- human monoclonal antibody (Catalog # MAB3598). Cells were stained using NorthernLights-557 goat anti- rat IgG secondary antibody (Catalog # NL013; red) and counterstained with DAPI (blue).

90 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Human None Polyclonal Goat IgG AF2459 100 µg $355 Repulsive Guidance Molecule A/RGM-A None Polyclonal Goat IgG AF2458 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF2458 50 µg $380 Human/Mouse None Polyclonal Sheep IgG AF3597 100 µg $355 RGM-B Mouse Biotin Polyclonal Sheep IgG BAF3597 50 µg $380

RGM-B in Mouse Brain. Repulsive Guidance Mole- cule B (RGM-B) was detected in a cryostat section of mouse brain (trigeminal ganglia) using anti-mouse RGM-B affinity-purified polyclonal antibody (Catalog # AF3597). Tissues were stained using anti-sheep HRP-DAB detection (brown) and counterstained with hematoxylin (blue).

Rheb Human/Mouse/Rat None 344912 Mouse IgG2B MAB3426 100 µg $240 Phospho-Ribosomal Protein S6 (S235/S236) Human/Mouse/Rat None Polyclonal Rabbit IgG AF3918 100 µg $285

Phospho-RPS6 in Human Colon Cancer. Phospho- Ribosomal Protein S6 (S235/S236) was detected in paraffin-embedded human colon cancer tissue sec- tions using anti-human/mouse/rat affinity-purified phospho-RPS6 (S235/S236) affinity-purified poly- clonal antibody (Catalog # AF3918). The tissue was stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counter- stained with hematoxylin (blue).

ROBO1 Rat None Polyclonal Goat IgG AF1749 100 µg $355 None Polyclonal Goat IgG AF3147 100 µg $355 ROBO2 Human

None 356001 Mouse IgG1 MAB3147 100 µg $240 None Polyclonal Goat IgG AF3076 100 µg $355 ROBO3 Human

None 327111 Mouse IgG2B MAB3076 100 µg $240

ROR/NR1F1-3 Pan Specific Human None H3925 Mouse IgG2A PP-H3925-00 100 µg $380 Phospho-RSK (S380) Pan Specific Human/Mouse/Rat None Polyclonal Rabbit IgG AF889 100 µg $285 Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Human/Mouse/Rat None Polyclonal Rabbit IgG AF892 100 µg $285 RSK1 Human/Mouse/Rat None Polyclonal Rabbit IgG AF992 100 µg $285 RSK2 Human/Mouse/Rat None Polyclonal Rabbit IgG AF1518 50 µg $285 Phospho-RSK3 (S218) Human/Mouse None Polyclonal Rabbit IgG AF893 100 µg $285 Human None Polyclonal Goat IgG AF2006 100 µg $355 RUNX2/CBFA1

Human/Mouse None 232902 Rat IgG2B MAB2006 100 µg $240 RUNX3/CBFA3 aa 186-415 Human None Polyclonal Goat IgG AF3765 100 µg $355

None K8508 Mouse IgG2A PP-K8508-00 100 µg $380 RXRa/NR2B1 Human

None K8508 Mouse IgG2A PP-K8508-10 100 µg $380 Ryk Mouse None Polyclonal Sheep IgG AF4649 100 µg $355 S100A2 Human None Polyclonal Goat IgG AF4870 100 µg $355

S100A4 Mouse None 438709 Rat IgG2B MAB4138 100 µg $240

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF3059 100 µg $355 S100A8 Mouse Biotin Polyclonal Goat IgG BAF3059 50 µg $380

None 335806 Rat IgG2B MAB3059 100 µg $240 S100A8 in Mouse NIH-3T3 Cells. S100A8 was de- tected in mouse NIH-3T3 embryonic fibroblast cell line using anti-mouse S100A8 monoclonal antibody (Catalog # MAB3059; red). Cells were counterstained with DAPI (blue).

Human None 474315 Mouse IgG2B MAB5578 100 µg $240 S100A9 Mouse None Polyclonal Goat IgG AF2065 100 µg $355

S100A9 in Mouse XB2 Cells. S100A9 was detected in mouse XB2 teratoma cell line using anti-mouse S100A9 affinity-purified polyclonal antibody (Catalog # AF2065). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Human None Polyclonal Goat IgG AF1698 100 µg $355 None Polyclonal Goat IgG AF2377 100 µg $355 S100A10 Mouse Biotin Polyclonal Goat IgG BAF2377 50 µg $380

None 344015 Rat IgG2A MAB2377 100 µg $240 S100A11 Human None Polyclonal Goat IgG AF4874 100 µg $355 S100A13 Mouse None Polyclonal Goat IgG AF4328 100 µg $355

S100A13 in Embryonic Mouse Cartilage. S100A13 was detected in a cryostat section of developing em- bryonic mouse (E13) cartilage using anti-mouse S100A13 affinity-purified polyclonal antibody (Cata- log # AF4328). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

S100A16 Human None Polyclonal Sheep IgG AF5180 100 µg $355

S100A16 in Human Brain Cortex. S100A16 was de- tected in a paraffin-embedded human brain cortex section using anti-human S100A16 affinity-purified polyclonal antibody (Catalog # AF5180). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). The section was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

S100B Human None Polyclonal Goat IgG AF1820 100 µg $355

92 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF2957 100 µg $355 S100P Human

None 357517 Mouse IgG1 MAB2957 100 µg $240 S100P in Human Liver. S100P was detected in par- affin-embedded human liver sections using anti- human S100P affinity-purified polyclonal antibody (Catalog # AF2957). Tissue was stained using anti- goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

SALL1 aa 258-499 Human None K9814 Mouse IgG2A PP-K9814-00 100 µg $380 d-Sarcoglycan Human None Polyclonal Goat IgG AF2778 100 µg $355 None Polyclonal Goat IgG AF1226 100 µg $355 Biotin Polyclonal Goat IgG BAF1226 50 µg $380 Sca-1/Ly6 Mouse

Biotin 177228 Rat IgG2A BAM1226 250 µg $380

None 177228 Rat IgG2A MAB1226 500 µg $325

SCARA5 Mouse None 462002 Rat IgG2A MAB4754 100 µg $240 Canine None Polyclonal Goat IgG AF2278 100 µg $355 SCF/c-kit Ligand Feline None Polyclonal Goat IgG AF2268 100 µg $355 Phospho-SCF R/c-kit (Y730) Human None Polyclonal Rabbit IgG AF3527 50 µg $285 Human None Polyclonal Goat IgG AF332 100 µg $355 None Polyclonal Goat IgG AF1356 100 µg $355 SCF R/c-kit Biotin Polyclonal Goat IgG BAF1356 50 µg $380 Mouse

Biotin 180627 Rat IgG2A BAM1356 250 µg $380

None 180627 Rat IgG2A MAB1356 500 µg $325 SCF R in Mouse Cerebellum. Stem Cell Factor Re- ceptor (SCF R) was detected in frozen sections of mouse cerebellum using anti-mouse SCF R monoclo- nal antibody (Catalog # MAB1356). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).

SCF R/c-kit aa 716-726 Human None Polyclonal Rabbit IgG AF3267 50 µg $285

SCGF/CLEC11a Human None 239029 Mouse IgG2A MAB1904 500 µg $325 SCL/Tal1 Human None Polyclonal Goat IgG AF3360 100 µg $355

SCL/Tal1 Human None 333803 Mouse IgG2B MAB3360 100 µg $240

Human None 294301 Mouse IgG2B MAB2357 100 µg $240 None Polyclonal Goat IgG AF2556 100 µg $355 SDNSF/MCFD2 Mouse Biotin Polyclonal Goat IgG BAF2556 50 µg $380

None 300812 Rat IgG2A MAB2556 100 µg $240

None BBIG-E6 Mouse IgG1 BBA1 100 µg $230

None BBIG-E4 Mouse IgG1 BBA16 200 µg $230 Human None Polyclonal Goat Serum BBA18 500 µL $195

E-Selectin/CD62E None BBIG-E4 Mouse IgG1 BBA2 1 mg $840 None Polyclonal Chicken IgY AF575 100 µg $355 Mouse Biotin Polyclonal Chicken IgY BAF575 50 µg $380 Rat None Polyclonal Goat IgG AF977 100 µg $355 None Polyclonal Goat IgG AF1534 100 µg $355 L-Selectin/CD62L Rat Biotin Polyclonal Goat IgG BAF1534 50 µg $380

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

P-Selectin/CD62P Human None 9.00E+01 Mouse IgG1 BBA30 200 µg $315 None Polyclonal Sheep IgG AF1728 100 µg $355 Semaphorin 3C Mouse Biotin Polyclonal Sheep IgG BAF1728 50 µg $380 Semaphorin 3E Human None Polyclonal Goat IgG AF3239 100 µg $355 Semaphorin 4D/CD100 Mouse None Polyclonal Sheep IgG AF5235 100 µg $355 Human None Polyclonal Goat IgG AF1146 100 µg $355 Semaphorin 6A None Polyclonal Goat IgG AF1615 100 µg $355 Mouse

None 201932 Rat IgG2A MAB1615 500 µg $325 Semaphorin 6B Human None Polyclonal Goat IgG AF2094 100 µg $355 None Polyclonal Goat IgG AF2108 100 µg $355 Semaphorin 6C Mouse

None 256631 Rat IgG2A MAB2108 500 µg $325 Serpin A4/Kallistatin Human None Polyclonal Goat IgG AF1669 100 µg $355 Serpin A6 Mouse None Polyclonal Goat IgG AF4065 100 µg $355 Serpin A8/Angiotensinogen Human None Polyclonal Goat IgG AF3156 100 µg $355 Serpin E1/PAI-1 Human None Polyclonal Goat IgG AF1786 100 µg $355 None Polyclonal Goat IgG AF1177 100 µg $355 Serpin F1/PEDF Human

None 187003 Mouse IgG2B MAB1177 500 µg $325 None Polyclonal Goat IgG AF1239 100 µg $355 Serpin F2/a2-Antiplasmin Mouse Biotin Polyclonal Goat IgG BAF1239 50 µg $380 Serum Amyloid A1 Mouse None Polyclonal Goat IgG AF2948 100 µg $355 Serum Amyloid A4 Human None Polyclonal Goat IgG AF3856 100 µg $355

Serum Amyloid A4 in Human Liver. Serum amyloid A4 (SAA4) was detected in paraffin-embedded human liver tissue sections using anti-human serum amyloid A4 affinity-purified polyclonal antibody (Catalog # AF3856) and Antigen Retrieval Reagent - Basic (Cata- log # CTS013). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

SF-1/NR5A1 Human None N1665 Mouse IgG1 PP-N1665-00 100 µg $380 None Polyclonal Goat IgG AF592 100 µg $355 sFRP-3 Mouse Biotin Polyclonal Goat IgG BAF592 50 µg $380 None Polyclonal Goat IgG AF2656 100 µg $355 SHBG Human Biotin Polyclonal Goat IgG BAF2656 50 µg $380

SHIP aa 874-941 Human/Mouse/Rat None 257812 Rat IgG2A MAB2317 100 µg $240

Human None 255402 Rat IgG2A MAB1878 100 µg $240 SHP-1 aa 205-595

Human/Mouse/Rat None 427043 Mouse IgG2B MAB18781 100 µg $240 SHP-1 in Human Thymus. Src-Homology 2 domain Phosphatase-1 (SHP-1) was detected in a paraffin- embedded human thymus section using anti- human/mouse/rat SHP-1 monoclonal antibody (Cat- alog # MAB18781). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Phospho-SHP-2 (Y542) Human/Mouse None Polyclonal Rabbit IgG AF3790 100 µg $285

Human None 219934 Mouse IgG1 MAB1968 100 µg $240 Siglec-2/CD22 Mouse None Polyclonal Goat IgG AF2296 100 µg $355

Siglec-5 Human None 194117 Mouse IgG2B MAB1072 500 µg $325

94 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Siglec-5/14 Human None Polyclonal Goat IgG AF1072 100 µg $355 None Polyclonal Goat IgG AF1138 100 µg $355 Siglec-7 Human

None 194212 Mouse IgG2B MAB1138 500 µg $325 None Polyclonal Goat IgG AF1139 100 µg $355 Siglec-9 Human Biotin Polyclonal Goat IgG BAF1139 50 µg $380 SKI Human None Polyclonal Goat IgG AF4025 100 µg $355 SLAIN1 aa 262-483 Human/Mouse None Polyclonal Goat IgG AF5638 100 µg $355

SLAIN1 in Mouse D3 Cells. SLAIN1 was detected in the D3 mouse embryonic stem cell line using anti- human/mouse SLAIN1 affinity-purified polyclonal antibody (Catalog # AF5638). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

None Polyclonal Goat IgG AF2798 100 µg $355 Sleeping Beauty Transposase Multi-species

None 324622 Mouse IgG1 MAB2798 100 µg $240

SLITRK1 Human None 327829 Mouse IgG2B MAB30091 100 µg $240 None Polyclonal Total Goat IgG AB-260-NA 1 mg $355 SLPI Human None Polyclonal Goat IgG AF1274 100 µg $355 Biotin Polyclonal Goat IgG BAF1274 50 µg $380 SMAC/Diablo Human None Polyclonal Goat IgG AF7891 100 µg $355 Smad1 aa 2-454 Human None Polyclonal Goat IgG AF2039 100 µg $355

Smad2 aa 20-108 Human None 376520 Mouse IgG2B MAB4037 100 µg $240

Smad3 aa 2-230 Human None 378611 Rat IgG1 MAB4038 100 µg $240 Smad3 in Human Pancreatic Cancer. Smad3 was detected in paraffin-embedded human pancreatic cancer sections using anti-human Smad3 monoclo- nal antibody (Catalog # MAB4038). Tissue was sub- jected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). Tissue was counter- stained with hematoxylin (blue).

Smad4 aa 139-332 Human None Polyclonal Goat IgG AF2097 100 µg $355

Smad4 aa 139-332 Human None 253343 Mouse IgG2B MAB2097 100 µg $240 Smad5 aa 155-258 Human None Polyclonal Goat IgG AF2250 100 µg $355

Smad5 in Human Colon. Smad5 was detected in paraffin-embedded human colon tissue sections using anti-human Smad5 affinity-purified polyclonal antibody (Catalog # AF2250). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

Smad7 aa 320-398 Human/Mouse/Rat None 293039 Mouse IgG2B MAB2029 100 µg $240 Smad7 in Human Liver Cancer. Smad7 was detect- ed in paraffin-embedded human liver cancer sec- tions using anti-human/mouse/rat Smad7 monoclo- nal antibody (Catalog # MAB2029). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Stain- ing Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Smad8 aa 158-223 Human None Polyclonal Goat IgG AF2309 100 µg $355 Human None Polyclonal Goat IgG AF3959 100 µg $355 SMAGP Rat None Polyclonal Goat IgG AF3967 100 µg $355

SMAGP in Human Colon. Small Transmembrane and Glycosylated Protein (SMAGP) was detected in paraf- fin-embedded human colon sections using anti- human SMAGP affinity-purified polyclonal antibody (Catalog # AF3959). Tissue was stained using the anti- goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

a -Smooth Muscle Actin N-terminal Human None 1A4 Mouse IgG MAB1420 100 µg $240 Synthetic Decapeptide 2A Snail Human None Polyclonal Goat IgG AF3639 100 µg $355 SNF1LK2 Human None Polyclonal Goat IgG AF5737 100 µg $355

SOCS-3 aa 1-225 Human/Mouse None 516919 Mouse IgG2B MAB5696 100 µg $240 SOCS-5 Human None Polyclonal Goat IgG AF4796 100 µg $355

SOD1/Cu-Zn SOD Human None 348808 Mouse IgG2A MAB3418 100 µg $240

SOD2/Mn-SOD aa 25-222 Human/Mouse/Rat None 349810 Mouse IgG1 MAB3419 100 µg $240

Somatostatin R2/SSTR2 Human None 402038 Mouse IgG2A MAB4224 100 µg $240 Sonic Hedgehog C-Terminus Mouse None Polyclonal Goat IgG AF445 100 µg $355 None Polyclonal Goat IgG AF464 100 µg $355 Sonic Hedgehog N-Terminus Mouse

None 171018 Rat IgG2A MAB4641 500 µg $325 SorCS1 aa 111-1099 Human None Polyclonal Goat IgG AF3457 100 µg $355 SorCS2 Mouse None Polyclonal Sheep IgG AF4237 100 µg $355 SorCS3 Mouse None Polyclonal Goat IgG AF3067 100 µg $355

SorCS3 aa 34-1122 Mouse None 339615 Rat IgG2A MAB30671 100 µg $240

SorLA Human None 525122 Mouse IgG1 MAB5699 100 µg $240 Human None Polyclonal Goat IgG AF3154 100 µg $355 Sortilin Mouse None Polyclonal Goat IgG AF2934 100 µg $355 None Polyclonal Goat IgG AF1589 100 µg $355 SOST/Sclerostin Mouse Biotin Polyclonal Goat IgG BAF1589 50 µg $380 SOX1 Human None Polyclonal Goat IgG AF3369 100 µg $355 None Polyclonal Goat IgG AF2018 100 µg $355 Human SOX2 Biotin Polyclonal Goat IgG BAF2018 50 µg $380

Human/Mouse None 245610 Mouse IgG2A MAB2018 100 µg $240

96 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

NL493 245610 Mouse IgG2A NL2018G 500 µL $345

SOX2 NorthernLights-conjugated Human/Mouse NL557 245610 Mouse IgG2A NL2018R 500 µL $345

NL637 245610 Mouse IgG2A NL2018V 500 µL $345 SOX2 in Human BG01V Cells. SOX2 was detected in the human BG01V embryonic stem cell line using anti-human/mouse SOX2 NorthernLights-637-conju- gated monoclonal antibody (Catalog # NL2018V; red). Cells were counterstained with DAPI (blue). The human BG01V embryonic stem cell line was grown on Irradiated Mouse Embryonic Fibroblasts (iMEF; Catalog # PSC001).

None Polyclonal Goat IgG AF2569 100 µg $355 SOX3 Human

None 287403 Mouse IgG2A MAB2569 100 µg $240 SOX7 Human None Polyclonal Goat IgG AF2766 100 µg $355 None Polyclonal Goat IgG AF3075 100 µg $355 SOX9 Human Biotin Polyclonal Goat IgG BAF3075 50 µg $380

SOX9 in Human HEK293 Cells. SOX9 was detected in human HEK293 embryonic kidney cell line using anti-human SOX9 affinity-purified polyclonal anti- body (Catalog # AF3075). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

SOX9 NorthernLights-conjugated Human NL557 Polyclonal Goat IgG NL3075R 500 µL $350 Human None Polyclonal Goat IgG AF2864 100 µg $355 SOX10

Human/Rat None 20B7 Mouse IgG1 MAB2864 100 µg $240

SOX10 NorthernLights-conjugated Human/Rat NL557 20B7 Mouse IgG1 NL2864R 500 µL $345 SOX15 Human None Polyclonal Sheep IgG AF4070 100 µg $355 None Polyclonal Goat IgG AF1924 100 µg $355 SOX17 Human Biotin Polyclonal Goat IgG BAF1924 50 µg $380 None 245013 Mouse IgG3 MAB1924 100 µg $240 SOX17 NorthernLights-conjugated, aa 177-144 Human NL557 Polyclonal Goat IgG NL1924R 500 µL $350

SOX17 in Mouse Melanoma Cells. SOX17 was de- tected in the B16 mouse melanoma cell line using anti-human SOX17 affinity-purified polyclonal anti- body (Catalog # AF1924). The cells were stained with NorthernLights-557 anti goat secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

SOX21 Human None Polyclonal Goat IgG AF3538 100 µg $355 None Polyclonal Goat IgG AF941 100 µg $355 Human

SPARC/Osteonectin None 122511 Mouse IgG1 MAB941 500 µg $325

Mouse None 124413 Rat IgG2B MAB942 500 µg $325 Human None Polyclonal Goat IgG AF2495 100 µg $355 Spinesin None Polyclonal Goat IgG AF1928 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1928 50 µg $380 Phospho-Src (Y419) Human None Polyclonal Rabbit IgG AF2685 100 µg $285

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

Src Human None 327554 Mouse IgG2A MAB3807 100 µg $240 Src aa 1-79 Human/Mouse/Rat None Polyclonal Goat IgG AF3389 100 µg $355

Src in Human Colon. Src was detected in paraffin- embedded normal human colon sections using anti- human/rat/mouse Src affinity-purified polyclonal­ antibody (Catalog # AF3389). Tissue was stained us- ing anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

SSEA-1 Human/Mouse None MC-480 Mouse IgM MAB2155 100 µg $240 SSEA-1 NorthernLights-conjugated Human/Mouse NL557 MC-480 Mouse IgM NL2155R 500 µL $345 SSEA-3 Human/Mouse None MC-631 Rat IgM MAB1434 100 µg $230 Biotin MC-813-70 Mouse IgG3 BAM1435 100 µg $295 SSEA-4 Human/Mouse None MC-813-70 Mouse IgG3 MAB1435 100 µg $230

STAT1 Human None 246523 Rat IgG2B MAB1490 100 µg $240

STAT2 Human None 545103 Mouse IgG2B MAB16661 100 µg $240 Phospho-STAT3 (Y705) Human None Polyclonal Rabbit IgG AF4607 100 µg $285

Phospho-STAT3 in Human Daudi Cells. Phospho- Signal Transducer and Activator of Transcription 3 (STAT3) was detected in human INF-a-treated Daudi human cells (Y705) treated with IFN-a using anti- human phospho-STAT3 affinity-purified polyclonal antibody (Catalog # AF4607). Cells were stained us­ing NorthernLights-557 donkey anti-rabbit IgG second- ary antibody (Catalog # NL004; red) and counter- stained with DAPI (blue).

None Polyclonal Sheep IgG AF17991 100 µg $355 STAT3 aa 1-175 Human

None 232209 Mouse IgG2B MAB1799 100 µg $240 STAT3 aa 688-770 Human/Mouse/Rat None Polyclonal Goat IgG AF1799 100 µg $355

STAT3 NorthernLights-conjugated Human/Mouse/Rat NL557 232209 Mouse IgG2B NL1799R 500 µL $345 Phospho-STAT5a/b (Y699) Human None Polyclonal Rabbit IgG AF4190 100 µg $285

Phospho-STAT5 (Y699) in IFN-α-treated Daudi cells. Phospho-Signal Transducer and Activator of Transcription 5 (Y699) was detected in IFN-a treated Daudi BurKitt’s lymphoma human cells using anti- human/mouse phosphor-STAT5 (Y699) polyclonal antibody (Catalog # AF4190). The cells were stained using NorthernLights-557 anti-rabbit secondary anti- body (Catalog # NL004; red) and counterstained with DAPI (blue).

Human None 251610 Mouse IgG1 MAB21741 100 µg $240 STAT5a aa 774-794 Human/Mouse None 251619 Mouse IgG3 MAB2174 100 µg $240

STAT6 aa 342-640 Human None 253906 Mouse IgG2A MAB2167 100 µg $240 STAT6 in Human Peripheral Blood Mononuclear Cells (PBMCs). Signal Transducer and Activator­ of Transcription 6 (STAT6) was detected in PHA-stimu- lated human PBMCs using anti-human/mouse STAT6 monoclonal antibody (Catalog # MAB2167; red). Cells were counterstained (green).

98 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF2566 100 µg $355 Stella/Dppa3 Mouse Biotin Polyclonal Goat IgG BAF2566 50 µg $380

None 283910 Rat IgG2A MAB2566 100 µg $240

STRA6 Human None 496613 Mouse IgG1 MAB5904 100 µg $240 STRO-1 Human None STRO-1 Mouse IgM MAB1038 100 µg $295

Substance P Human/Mouse/Rat None 266815 Mouse IgG1 MAB4375 100 µg $240

SUMO3 Human None 401513 Rat IgG2A MAB2959 100 µg $240 Survivin Human None Polyclonal Rabbit IgG AF886 100 µg $285

SUZ12 Human None 448909 Mouse IgG1 MAB4184 100 µg $240 Synapsin I Pan Specific Human/Mouse/Rat/Bovine None Polyclonal Rabbit IgG PPS035 10 µg $355 Synaptophysin Human None Polyclonal Goat IgG AF5555 100 µg $355

None ASV48 Mouse IgG2B MAB4364 100 µg $240 Synaptotagmin Rat

None ASV30 Mouse IgG2A MAB43641 100 µg $240 Synaptotagmin I in Rat Spinal Cord. Synapto­ tagmin I was detected in a cryostat section of rat spi- nal cord using anti-rat Synaptotagmin I monoclonal antibody (Catalog # MAB43641; red). The tissue was counterstained green.

Syndecan-1/CD138 Human None Polyclonal Goat IgG AF2780 100 µg $355 Biotin Polyclonal Goat IgG BAF2780 50 µg $380 Human

None 359103 Rat IgG1 MAB2780 100 µg $240 Syndecan-1/CD138 None Polyclonal Goat IgG AF3190 100 µg $355 Mouse

None 300506 Rat IgG1 MAB2966 100 µg $240 None Polyclonal Goat IgG AF2965 100 µg $355 Syndecan-2 Human

None 305507 Rat IgG2A MAB29651 100 µg $240

Human None 374412 Rat IgG2A MAB35391 100 µg $240 Syndecan-3 Mouse None Polyclonal Goat IgG AF2734 100 µg $355 Syndecan-3 Isoform 1 Human None Polyclonal Goat IgG AF3539 100 µg $355 Syndecan-4 Human None Polyclonal Goat IgG AF2918 100 µg $355 Syntaxin-BP1 Human/Mouse/Rat None Polyclonal Goat IgG AF5675 100 µg $355 Synuclein-a Human None Polyclonal Goat IgG AF1338 100 µg $355 None Polyclonal Sheep IgG AF5528 100 µg $355 Synuclein-b Human

None 516505 Mouse IgG2B MAB5528 100 µg $240

Synuclein-g Human None 514304 Mouse IgG1 MAB5745 100 µg $240 TAB1 aa 7-159 Human/Mouse None Polyclonal Goat IgG AF3578 100 µg $355 TACE/ADAM17 Cytosolic Domain Human None Polyclonal Goat IgG AF2129 100 µg $355 None Polyclonal Chicken IgY AF930 100 µg $355 TACE/ADAM17 Ectodomain Human Biotin Polyclonal Chicken IgY BAF930 50 µg $380 None Polyclonal Goat IgG AF174 100 µg $355 Human Biotin Polyclonal Goat IgG BAF174 50 µg $380

TACI/TNFRSF13B None 165609 Mouse IgG1 MAB174 500 µg $325 None Polyclonal Goat IgG AF1041 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1041 50 µg $380 Tau Multi-species None Polyclonal Goat IgG AF3494 100 µg $355

T-bet/TBX21 Human None 525803 Mouse IgG1 MAB5385 100 µg $240

TBX2 Human None 393720 Rat IgG2A MAB50401 100 µg $240 TBX3 aa 321-483 Human None Polyclonal Goat IgG AF4509 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE TBX6 Human None Polyclonal Goat IgG AF4744 100 µg $355

TBX6 in Mouse Embryonic Mesoderm. T-box Pro- tein 6 (TBX6) was detected in E9.5 mouse embryonic tailbud presomitic mesoderm sections using anti- human TBX6 affinity-purified polyclonal antibody­ (Catalog # AF4744). Cells were stained using NorthernLights-557 donkey anti-goat IgG second- ary antibody (Catalog # NL001; red) and counter- stained with DAPI (blue).

TC21/R-Ras2 Human/Rat None Polyclonal Goat IgG AF3605 100 µg $355 TCCR/WSX-1 Human None Polyclonal Goat IgG AF1479 100 µg $355 TCF-2/HNF-1b Human None Polyclonal Goat IgG AF3330 100 µg $355 TCF-7/TCF-1 Human None Polyclonal Goat IgG AF5596 100 µg $355 TCL1A Human None Polyclonal Goat IgG AF4847 100 µg $355 TEM8 Isoform 1 Human None Polyclonal Goat IgG AF3886 100 µg $355 None Polyclonal Goat IgG AF3358 100 µg $355 Human

Tenascin C None 391819 Rat IgG2A MAB3358 100 µg $240

Human/Mouse None 578 Rat IgG2A MAB2138 100 µg $240 Human None Polyclonal Goat IgG AF3865 100 µg $355 Tenascin R

Mouse/Rat None 619 Mouse IgG1 MAB1624 100 µg $240 Tenascin R in Human Brainstem. Tenascin R was detected in paraffin-embedded human brainstem (medulla) tissue sections using anti-human Tenascin R affinity-purified polyclonal antibody (Catalog # AF3865). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

Biotin TER-119 Rat IgG2B BAM1125 250 µg $380 TER-119 Mouse

None TER-119 Rat IgG2B MAB1125 500 µg $325

TFF3 Human None 415909 Mouse IgG1 MAB4407 100 µg $240 TFF3 in Human Colon. Trefoil factor 3 (TFF3) was detected in a paraffin-embedded human colon sec- tion using anti-human TFF3 monoclonal antibody (Catalog # MAB4407). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

TFPI-2 Human None 243220 Mouse IgG2A MAB2545 500 µg $325 None Polyclonal Total Goat IgG AB-239-NA 1 mg $355 TGF-a Human None Polyclonal Goat IgG AF-239-NA 100 µg $355 None Polyclonal Total Goat IgG AB-246-NA 1 mg $355 LAP TGF-b1 Human None Polyclonal Goat IgG AF-246-NA 100 µg $355

TGF-b1 Multi-species None 9016 Mouse IgG1 MAB240 500 µg $325

TGF-b1, 2, 3 Multi-species None 1D11 Mouse IgG1 MAB1835 500 µg $325

TGF-b2 Multi-species None 8607 Mouse IgG2B MAB612 500 µg $325 TGF-b2/1.2 Multi-species None Polyclonal Goat IgG AF-302-NA 100 µg $355 None Polyclonal Goat IgG AF-243-NA 100 µg $355

TGF-b3 Multi-species None 20724 Mouse IgG1 MAB243 500 µg $325

None 44922 Mouse IgG1 MAB643 500 µg $325 100 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE TGF-b RI/ALK-5 Human None Polyclonal Goat IgG AF3025 100 µg $355 TGF-b RII Human None Polyclonal Goat IgG AF-241-NA 250 µg $355 Thioredoxin-1 Human None Polyclonal Goat IgG AF1970 100 µg $355

Thioredoxin Reductase 2/TRXR2 aa 268-393 Human/Mouse None 575322 Mouse IgG1 MAB5815 100 µg $240 Thimet Oligopeptidase/THOP1 Human None Polyclonal Sheep IgG AF3439 100 µg $355

Human None 501733 Mouse IgG1 MAB3947 100 µg $240 Thrombomodulin/THBD/CD141 None Polyclonal Goat IgG AF3894 100 µg $355 Mouse

None 461714 Rat IgG2B MAB3894 100 µg $240

Thrombospondin-1 Human None 301221 Mouse IgG2B MAB3074 500 µg $325 None Polyclonal Goat IgG AF619 100 µg $355 Tie-1 Human

None 88016 Mouse IgG1 MAB619 500 µg $325 Phospho-Tie-2 (Y1100) Human/Mouse None Polyclonal Rabbit IgG AF3909 100 µg $285 Human None Polyclonal Goat IgG AF313 100 µg $355 Tie-2 Zebrafish None Polyclonal Goat IgG AF928 100 µg $355

Tie-2 in Developing Blood Vessels of Zebrafish. Tie-2 was detected in the blood vessels of an embry- onic zebrafish using anti-zebrafish Tie-2 affinity-puri- fied polyclonal antibody (Catalog # AF928). The tissue was stained using NorthernLights-557 donkey anti- goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Human None 219211 Mouse IgG2B MAB1750 500 µg $325 TIM-1/KIM-1/HAVCR Rat None Polyclonal Goat IgG AF3689 100 µg $355

TIM-1 in Rat Kidney. T cell Immunoglobulin and Mu- cin domain-containing (TIM-1), also known as Kidney Injury Molecule 1 (KIM-1), was detected in frozen sec- tions of rat kidney using anti-rat TIM-1 affinity-puri- fied polyclonal antibody (Catalog # AF3689). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counter- stained with hematoxylin (blue).

Human None 63515 Mouse IgG2B MAB970 500 µg $325 TIMP-1 Mouse None Polyclonal Goat IgG AF980 100 µg $355 Rat None Polyclonal Goat IgG AF580 100 µg $355 None Polyclonal Goat IgG AF971 100 µg $355 TIMP-2 Human

None 89025 Mouse IgG1 MAB971 500 µg $325 TIMP-2 in Human Ovarian Cancer. Tissue Inhibitor of Metalloproteinase 2 (TIMP-2) was detected in a paraffin-embedded tissue section of human ovarian cancer using anti-human TIMP-2 affinity-purified polyclonal antibody (Catalog # AF971). Tissues were stained with the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown), and counter- stained with hematoxylin (blue).

TIMP-3 Human None 183551 Mouse IgG1 MAB973 500 µg $325

TIMP-4 Human None 153934 Mouse IgG1 MAB974 500 µg $325 TLR4 Human None Polyclonal Goat IgG AF1478 100 µg $355 None Polyclonal Goat IgG AF1995 100 µg $355 TMEFF1/Tomoregulin-1 Mouse Biotin Polyclonal Goat IgG BAF1995 50 µg $380

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF2279 100 µg $355 Bovine Biotin Polyclonal Goat IgG BAF2279 50 µg $380 None Polyclonal Goat IgG AF1507 100 µg $355 Canine Biotin Polyclonal Goat IgG BAF1507 50 µg $380

None 236812 Mouse IgG1 MAB1507 500 µg $325 Equine None Polyclonal Goat IgG AF1814 100 µg $355 TNF-a None Polyclonal Goat IgG AF-210-NA 100 µg $355 Human Biotin Polyclonal Goat IgG BAF210 50 µg $380

None 28401 Mouse IgG1 MAB610 500 µg $325 Mouse None Polyclonal Goat IgG AF-410-NA 100 µg $355 None Polyclonal Goat IgG AF690 100 µg $355 Porcine

None 103302 Mouse IgG1 MAB6903 100 µg $240 Rat None Polyclonal Goat IgG AF-510-NA 100 µg $355

Human None 5802 Mouse IgG1 MAB211 500 µg $325 TNF-b/TNFSF1 Mouse Biotin Polyclonal Goat IgG BAF749 50 µg $380 Human None Polyclonal Goat IgG AF225 100 µg $355 TNF RI/TNFRSF1A Mouse None Polyclonal Goat IgG AF-425-PB 100 µg $355

TNF RI in Human Peripheral Blood Lymphocytes. Tumor Necrosis Factor Receptor I (TNF RI) was de- tected in human peripheral blood lymphocytes using anti-human TNF RI affinity-purified polyclonal anti- body (Catalog # AF225; red) and cells were counter- stained green.

None Polyclonal Total Goat IgG AB-226-PB 1 mg $355 Human

TNF RII/TNFRSF1B None 22221 Mouse IgG2A MAB226 500 µg $325 Mouse None Polyclonal Goat IgG AF-426-PB 100 µg $355

TOR Human/Mouse None 303728 Rat IgG2A MAB1537 100 µg $240 TOR in HEK293 cells. Target of rapamycin (TOR) ex- pression was detected in HEK293 human embryonic kidney cells using anti-human TOR monoclonal anti- body (Catalog # MAB1537). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary anti- body (Catalog # NL013; red) and counterstained with DAPI (blue).

TRa/NR1A1 Human None H2804 Mouse IgG2A PP-H2804-00 100 µg $380

TR4/NR2C2 Human None H0107B Mouse IgG2A PP-H0107B-00 100 µg $380

Biotin TRA-1-85 Mouse IgG1 BAM3195 100 µg $240 TRA-1-85 Human

None TRA-1-85 Mouse IgG1 MAB3195 100 µg $240 TRADD Human None Polyclonal Goat IgG AF2658 100 µg $355 None Polyclonal Goat IgG AF375 100 µg $355

Human None 75411 Mouse IgG1 MAB375 500 µg $325

TRAIL/TNFSF10 None 75402 Mouse IgG1 MAB687 500 µg $325 None Polyclonal Goat IgG AF1121 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1121 50 µg $380 TRAIL R1/TNFRSF10A Human None Polyclonal Goat IgG AF347 100 µg $355

TRAIL R2/TNFRSF10B Mouse None 118929 Rat IgG2A MAB1540 500 µg $325

102 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF630 100 µg $355 TRAIL R3/TNFRSF10C Human

None 90905 Mouse IgG1 MAB6301 500 µg $325 TRAIL Receptor 3 in Human Spleen. Tumor Necro- sis Factor-related Apoptosis-inducing Ligand Recep- tor 3 (TRAIL R3) was detected in paraffin-embedded human spleen tissue sections using anti-human TRAIL Receptor 3 monoclonal antibody (Catalog # MAB630). Tissues were subjected to antigen retrieval using basic reagent (Catalog # CTS013) and stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). Tissues were counter- stained with hematoxylin (blue).

TRAIL R4/TNFRSF10D Human None Polyclonal Goat IgG AF633 100 µg $355

TRANCE/TNFSF11/RANK L Human None 70525 Mouse IgG2B MAB626 500 µg $325 TRANCE/TNFSF11/RANK L Mouse None Polyclonal Goat IgG AF462 100 µg $355 TfR (Transferrin R) Human None Polyclonal Goat IgG AF2474 100 µg $355 None Polyclonal Goat IgG AF1187 100 µg $355

TREM-1 Mouse Biotin 174031 Rat IgG2A BAM1187 100 µg $380

None 174031 Rat IgG2A MAB1187 500 µg $325 None Polyclonal Goat IgG AF1828 100 µg $355 Human Biotin Polyclonal Goat IgG BAF1828 50 µg $380 TREM-2 None Polyclonal Sheep IgG AF1729 100 µg $355 Mouse Biotin Polyclonal Sheep IgG BAF1729 50 µg $380 Human None Polyclonal Goat IgG AF175 100 µg $355 TrkA None Polyclonal Goat IgG AF1056 100 µg $355 Rat Biotin Polyclonal Goat IgG BAF1056 50 µg $380

Human None 75133 Mouse IgG2B MAB397 500 µg $325 None Polyclonal Goat IgG AF1494 100 µg $355 TrkB Mouse Biotin Polyclonal Goat IgG BAF1494 50 µg $380

None 225105 Rat IgG2B MAB1494 500 µg $325 None Polyclonal Goat IgG AF373 100 µg $355 Human

None 75219 Mouse IgG2B MAB373 500 µg $325 TrkC None Polyclonal Goat IgG AF1404 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1404 50 µg $380

TrkC in Rat Spinal Cord. TrkC was detected in a cryostat tissue section of rat spinal cord using anti- human TrkC monoclonal antibody (Catalog # MAB373; red).

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF650 100 µg $355 Human Biotin Polyclonal Goat IgG BAF650 50 µg $380 TROP-2 None Polyclonal Goat IgG AF1122 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF1122 50 µg $380

Troponin T (Cardiac) Human None 200805 Mouse IgG2A MAB1874 100 µg $240 None Polyclonal Goat IgG AF723 100 µg $355 TROY/TNFRSF19 Mouse Biotin Polyclonal Goat IgG BAF723 50 µg $380 Trypsin Pan Specific (PRSS1/2/3) Human None Polyclonal Sheep IgG AF3586 100 µg $355 Trypsin 1/PRSS1 Human None Polyclonal Sheep IgG AF3848 100 µg $355 None Polyclonal Goat IgG AF3565 100 µg $355 Trypsin 3/PRSS3 Mouse

None 419911 Rat IgG1 MAB3565 100 µg $240 Trypsin 3 in Mouse Pancreas. Trypsin 3 was detect- Trypsin-3 in Mouse Kidney. Trypsin-3 was detected ed in a frozen section of mouse pancreas using anti- in frozen mouse kidney sections using anti-mouse mouse Trypsin-3 monoclonal antibody (Catalog # Trypsin-3 monoclonal antibody (Catalog # MAB3565). MAB3565). Tissues were stained using anti-rat HRP- Tissue was stained using the anti-rat HRP-DAB Cell DAB reagents (brown) and counterstained with he- and Tissue Staining Kit (Catalog # CTS017; brown) matoxylin (blue). and counterstained with hematoxylin (blue).

Tryptophan Hydroxylase Pan Specific Multi-species None Polyclonal Sheep IgG PPS038 100 µL $355 Tryptophan Hydroxylase 1 Human None Polyclonal Goat IgG AF5276 100 µg $355 TSG Mouse None Polyclonal Goat IgG AF756 100 µg $355

Biotin 120024 Rat IgG2A BAM756 250 µg $380 TSG Mouse

None 120024 Rat IgG2A MAB756 500 µg $325 Human None Polyclonal Goat IgG AF2104 100 µg $355 TSG-6 None Polyclonal Goat IgG AF2326 100 µg $355 Mouse Biotin Polyclonal Goat IgG BAF2326 50 µg $380

TSH b Human None 512913 Mouse IgG1 MAB5794 100 µg $240 TSLP R Human None Polyclonal Goat IgG AF981 100 µg $355

TSPAN8 Human None 458811 Rat IgG2B MAB4734 100 µg $240

Biotin TuJ-1 Mouse IgG2A BAM1195 100 µg $380 Neuron-specific b-III Tubulin Multi-species

None TuJ-1 Mouse IgG2A MAB1195 100 µg $240 b-III Tubulin and Nestin in Rat Cortical Stem Cells. b-III Tubulin and GFAP in Adult Mouse Brain. Neu- b-III Tubulin and Nestin were detected in rat cortical ronal b-III Tubulin and astrocytic GFAP were detected stem cells (Catalog # NSC001) using anti-neuron spe- in adult mouse brain sections using anti-b-III Tubulin cific b-III Tubulin monoclonal (clone TuJ-1) antibody monoclonal antibody (Catalog # MAB1195; Tuj1) and (Catalog # MAB1195; red) and anti rat Nestin affinity- anti-human GFAP affinity-purified polyclonal anti- purified polyclonal antibody (Catalog # AF2736; body (Catalog # AF2594). The tissue was stained us- green). Cells were stained with NorthernLights-557 ing NorthernLights-493 donkey anti-mouse (Catalog donkey anti-mouse IgG (Catalog # NL007) and # NL009; green) and NorthernLights-557 donkey an- NorthernLights-493 donkey anti-goat IgG (Catalog # ti-sheep IgG (Catalog # NL010; red) secondary anti- NL003) secondary antibodies. Tissue was counter- bodies, respectively. The nuclei were stained using stained with DAPI (blue). DAPI (blue)

NL557 TuJ-1 Mouse IgG NL1195R 500 µL $345 Neuron-specific b-III Tubulin 2A NorthernLights-conjugated Multi-species NL637 TuJ-1 Mouse IgG2A NL1195V 500 µL $345 TWEAK/TNFSF12 Human Biotin Polyclonal Goat IgG BAF1090 50 µg $380 Human None Polyclonal Goat IgG AF1199 100 µg $355 TWEAK R/TNFRSF12 Mouse None Polyclonal Goat IgG AF1610 100 µg $355

None 179003 Mouse IgG1 MAB1676 500 µg $325 Phospho-Phospho-Tyrosine Multi-species

None 216954 Rat IgG2A MAB16761 500 µg $325

104 For research use only. Not for use in diagnostic procedures. R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE Phospho-Tyrosine Hydroxylase (S19) Rat None Polyclonal Rabbit IgG PPS087 100 µL $355 Phospho-Tyrosine Hydroxylase (S31) Mouse/Rat/Primate None Polyclonal Rabbit IgG PPS088 100 µL $355 Phospho-Tyrosine Hydroxylase (S40) Rat None Polyclonal Rabbit IgG PPS041 100 µL $355

None TH-2 Mouse IgG1 MAB1423 100 µg $240 Tyrosine Hydroxylase Multi-species

None 6D7 Mouse IgG1 PPS040 100 µL $355

Ubiquitin/Ubiquitin+1 Human None 83406 Mouse IgG2B MAB701 100 µg $240

Ubiquitin+1 Human None 82001 Mouse IgG2A MAB703 100 µg $240 ULBP-2 Human None Polyclonal Goat IgG AF1298 100 µg $355 UNC5H1 Rat None Polyclonal Goat IgG AF1405 100 µg $355 None Polyclonal Goat IgG AF1006 100 µg $355 UNC5H2 Rat Biotin Polyclonal Goat IgG BAF1006 50 µg $380 None Polyclonal Goat IgG AF1005 100 µg $355 UNC5H3 Human

None 200414 Mouse IgG2B MAB1005 500 µg $325 u-Plasminogen Activator (uPA)/Urokinase Human None Polyclonal Goat IgG AF1310 100 µg $355 uPAR Mouse None Polyclonal Goat IgG AF534 100 µg $355 Uromodulin Human None Polyclonal Sheep IgG AF5144 100 µg $355

Uromodulin in Human Kidney. Uromodulin was detected in paraffin-embedded human kidney sec- tions using anti-human Uromodulin affinity-purified polyclonal antibody (Catalog # AF5144). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counter- stained with hematoxylin (blue).

Uteroglobin/SCGB1A1 Human None 394324 Rat IgG1 MAB4218 100 µg $240 UTF1 Human None Polyclonal Goat IgG AF3958 100 µg $355 VAMP-1 Human/Mouse None Polyclonal Goat IgG AF4828 100 µg $355

VAMP-1 in Mouse Brain. Vesicle-associated mem- brane protein 1 (VAMP-1) was detected in frozen sec- tions of mouse brain (spinal cord) using anti-human/ mouse VAMP-1 affinity-purified polyclonal antibody (Catalog # AF4828). The tissue was stained using anti- goat IgG NorthernLights-557-conjugat­ed secondary antibody (Catalog # NL001; red) and counterstained green.

Vang-like Protein 1/VANGL1 Human None 497707 Mouse IgG2B MAB5476 100 µg $240 Vanilloid R1/TRPV1 Rat None Polyclonal Goat IgG AF3066 100 µg $355

Vanilloid R1 in Rat Dorsal Root Ganglion. Vanilloid R1 was detected in a frozen section of rat dorsal root ganglion using anti-rat Vanilloid R1 affinity-purified polyclonal antibody (Catalog # AF3066; red) and counterstained green.

Vanilloid R-like 3/TRPV3 Human None Polyclonal Sheep IgG AF4167 100 µg $355 VASA Human None Polyclonal Goat IgG AF2030 100 µg $355

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat Serum BBA19 500 µL $195

Human None BBIG-V1 Mouse IgG1 BBA5 100 µg $230 VCAM-1/CD106

None BBIG-V1 Mouse IgG1 BBA6 1 mg $840 Mouse None Polyclonal Goat IgG AF643 100 µg $355

VDR/NR1I1 Human None H4537 Mouse IgG2A PP-H4537-00 100 µg $380

Canine None 247109 Mouse IgG1 MAB1603 500 µg $325 VEGF

Human None 26503 Mouse IgG2B MAB293 500 µg $325 None Polyclonal Goat IgG AF1603 100 µg $355 Canine Biotin Polyclonal Goat IgG BAF1603 50 µg $380 VEGF 164 Mouse None Polyclonal Goat IgG AF-493-NA 100 µg $355 Rat None Polyclonal Goat IgG AF564 100 µg $355

VEGF in Mouse Kidney. Vascular Endothelial Growth VEGF in Rat Kidney. Vascular Endothelial Growth Factor (VEGF) was detected in a cryostat section of Factor (VEGF) was detected in a cryostat section of rat mouse kidney glomerulus using anti-mouse VEGF kidney glomerulus using anti-rat VEGF 164 affinity- affinity-purified polyclonal antibody (Catalog # AF- purified polyclonal antibody (Catalog # AF564). 493-NA; red) and counterstained green. Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

None Polyclonal Total Goat IgG AB-293-NA 1 mg $355 VEGF 165 Human None Polyclonal Goat IgG AF-293-NA 100 µg $355

VEGF 165b Human None 56-1 Mouse IgG1 MAB3045 500 µg $325

VEGF-B 167/186 Human/Mouse None 58013 Mouse IgG1 MAB751 500 µg $325 VEGF-B 186 Mouse None Polyclonal Goat IgG AF767 100 µg $355 VEGF-C Human None Polyclonal Goat IgG AF752 100 µg $355 None Polyclonal Goat IgG AF286 100 µg $355 Human

VEGF-D None 78923 Mouse IgG1 MAB286 500 µg $325 Mouse None Polyclonal Goat IgG AF469 100 µg $355 None Polyclonal Goat IgG AF321 100 µg $355 Human VEGF R1/Flt-1 Biotin Polyclonal Goat IgG BAF321 50 µg $380

Mouse None 141515 Rat IgG2A MAB471 500 µg $325 None Polyclonal Goat IgG AF357 100 µg $355 Human

None 89115 Mouse IgG1 MAB3571 500 µg $325 VEGF R2/KDR/Flk-1 None Polyclonal Goat IgG AF644 100 µg $355 Mouse

None 522302 Rat IgG1 MAB4432 100 µg $240 None Polyclonal Goat IgG AF349 100 µg $355 VEGF R3/Flt-4 Human

None 54703 Mouse IgG1 MAB3491 500 µg $325 Versican Isoform V0 Human None Polyclonal Goat IgG AF3054 100 µg $355

VG5Q Mouse None 368011 Rat IgG2A MAB30481 100 µg $240

VHR Human None 237018 Mouse IgG1 MAB16541 100 µg $240 None Polyclonal Goat IgG AF2105 100 µg $355 Vimentin Human

None 280618 Rat IgG2A MAB2105 100 µg $240 Vimentin in Human Lung Carcinoma Cells. Vimen- tin was detected in cultured A549 human lung carci- noma cells cells using anti-human Vimentin mono- clonal antibody (Catalog # MAB2105). The Cells were stained using NorthernLights-493 anti-rat secondary antibodies (Catalog # NL015; green) and the nuclei counterstained with DAPI (blue).

106 For research use only. Not for use in diagnostic procedures. IHC/ICC SUPPLEMENTAL REAGENTS

MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE

VIP R2 Human None 476031 Mouse IgG1 MAB5416 100 µg $240

Human None 342603 Mouse IgG2B MAB2349 100 µg $240 Vitronectin

Mouse None 347317 Rat IgG2A MAB38751 100 µg $240 VLDL R Mouse None Polyclonal Goat IgG AF2258 100 µg $355 VSIG1 Human None Polyclonal Sheep IgG AF4818 100 µg $355

VSIG1 in Human Testis. V-set and Ig domain-con- taining protein 1 (VSIG1) was detected in frozen a section of human testis using anti-human VSIG1 affinity-purified polyclonal antibody (Catalog # AF4818). Cells were stained using Northern- Lights-557 donkey anti-sheep IgG secondary anti- body (Catalog # NL010; red) and counterstained with DAPI (blue).

Human None 336925 Mouse IgG1 MAB3070 100 µg $240 WASP Human/Mouse None Polyclonal Goat IgG AF3070 100 µg $355

None 133015 Mouse IgG1 MAB134-050 50 µg $150 WIF-1 Human

None 133015 Mouse IgG1 MAB134-500 500 µg $325 Human None Polyclonal Goat IgG AF1627 100 µg $355 WISP-1/CCN4 None Polyclonal Sheep IgG AF1680 100 µg $355 Mouse Biotin Polyclonal Sheep IgG BAF1680 50 µg $380 Wnt-1 Mouse None Polyclonal Goat IgG AF1620 100 µg $355 Wnt-2 Human None Polyclonal Goat IgG AF3464 100 µg $355

Wnt-2 in Human Stomach Cancer. Wnt-2 was de- tected in a paraffin-embedded human stomach can- cer section using anti-human Wnt-2 affinity-purified polyclonal antibody (Catalog # AF3464). The section was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). The tissue was coun- terstained with hematoxylin (blue).

Wnt-2b Mouse None Polyclonal Goat IgG AF3900 100 µg $355 Wnt-4 Mouse None Polyclonal Goat IgG AF475 100 µg $355 Wnt-5a Mouse None Polyclonal Goat IgG AF645 100 µg $355

Wnt-5a in Embryonic Mouse Rib. Wnt-5a was de- tected in a paraffin-embedded tissue section of E15 mouse rib using anti-mouse Wnt-5a affinity-purified polyclonal antibody (Catalog # AF645). The tissue was stained using the anti-goat HRP-DAB (brown) Cell and Tissue Staining Kit (Catalog # CTS008) and coun- terstained with hematoxylin (blue).

Wnt-5b Mouse None 325004 Rat IgG2A MAB3006 100 µg $240 Wnt-6 Human None Polyclonal Sheep IgG AF4109 100 µg $355 Wnt-7a Human None Polyclonal Goat IgG AF3008 100 µg $355 Wnt-7b Human None Polyclonal Goat IgG AF3460 100 µg $355 Wnt-8a Mouse None Polyclonal Goat IgG AF2248 100 µg $355

Wnt-8b Human/Mouse None 341504 Rat IgG2A MAB3367 100 µg $240

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MOLECULE SPECIES LABEL CLONE HOST/TYPE CATALOG # SIZE PRICE None Polyclonal Goat IgG AF3157 100 µg $355 Wnt-9a Human

None 326707 Mouse IgG2A MAB31571 100 µg $240 Wnt-9a in Human Breast Cancer. Wnt-9a was de- tected in a paraffin-embedded human breast cancer section using anti-human Wnt-9a monoclonal anti- body (Catalog # MAB31571). The section was sub- jected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Cata- log # CTS002; brown). The tissue was counterstained with hematoxylin (blue).

Wnt-9b Mouse None Polyclonal Goat IgG AF3669 100 µg $355 Wnt-11 Mouse None Polyclonal Goat IgG AF2647 100 µg $355 None Polyclonal Goat IgG AF486 100 µg $355 XCL1/Lymphotactin Mouse Biotin Polyclonal Goat IgG BAF486 50 µg $380 XCR1 Human None Polyclonal Goat IgG AF857 100 µg $355

Human None 117318 Mouse IgG1 MAB8221 100 µg $240 XIAP Human/Mouse/Rat None Polyclonal Goat IgG AF8221 100 µg $355

Human/Mouse/Rat None 339827 Mouse IgG2B MAB32051 100 µg $240 Yes aa 1-88 Human/Rat None Polyclonal Goat IgG AF3205 100 µg $355 ZAP70 aa 163-254 Human None Polyclonal Goat IgG AF3709 100 µg $355

ZAP70 in Human Lymphoma. Zeta-chain (TCR) associated protein kinase 70 (ZAP70) was detected in a paraffin-embedded human lymphoma section using anti-human ZAP70 affinity-purified polyclonal antibody (Catalog # AF3709). The tissue was subject- ed to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Cata- log # CTS008; brown). The section was counter- stained with hematoxylin (blue).

ZIC1 Human/Mouse None Polyclonal Goat IgG AF4978 100 µg $355

ZIC1 in Embryonic Mouse Brain. Zinc-finger protein of the cerebellum 1 (ZIC1) was detected in a frozen section of E13 mouse brain using anti-human ZIC1 affinity-purified polyclonal antibody (Catalog # AF4978). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxy- lin (blue).

ZNF24 Human None Polyclonal Goat IgG AF3637 100 µg $355 ZNF206 Human None Polyclonal Goat IgG AF4020 100 µg $355

108 For research use only. Not for use in diagnostic procedures. IHC/ICC SUPPLEMENTAL REAGENTS

Secondary & Control Antibodies for IHC/ICC SECONDARY ANTIBODIES analyte label type applications catalog # size price None Chicken IgY specific polyclonal goat IgG Western blot AF010 500 µg $75 Chicken IgY Biotin Chicken IgY specific polyclonal goat IgG Western blot BAF010 250 µg $75 None Goat IgG specific polyclonal donkey IgG Western blot AF109 500 µg $75 Biotin Goat IgG specific polyclonal donkey IgG Western blot BAF109 250 µg $75 Biotin Goat IgG specific polyclonal rabbit IgG Western blot BAF017 250 µg $75 Biotin Goat IgG specific polyclonal chicken IgY Western blot BAF019 250 µg $75 HRP Goat IgG specific polyclonal donkey IgG Western blot HAF109 1 mL $95 Goat IgG HRP Goat IgG specific polyclonal rabbit IgG Western blot HAF017 1 mL $95 HRP Goat IgG specific polyclonal chicken IgG Western blot HAF019 1 mL $95 APC Goat IgG specific polyclonal donkey IgG Flow Cytometry F0108 100 Tests $220 Fluorescein Goat IgG specific polyclonal donkey IgG Flow Cytometry F0109 100 Tests $140 PE Goat IgG specific polyclonal donkey IgG Flow Cytometry F0107 100 Tests $175 None Goat IgG specific polyclonal rabbit IgG Western blot R-401-C-ABS 500 µg $75 Goat IgG (H+L) PerCP Goat IgG specific polyclonal donkey IgG (H+L) chains Flow Cytometry F0124 100 Tests $190

APC Monoclonal mouse IgG2B, clone # MAH1.12 Flow Cytometry F0121 100 Tests $220

PE Monoclonal mouse IgG2B, clone # MAH1.12 Flow Cytometry F0120 100 Tests $175 Hamster IgG

None Monoclonal mouse IgG2B, clone # MAH1.12 Western blot MAB011 500 µg $165

Biotin Monoclonal mouse IgG2B, clone # MAH1.12 Western blot BAM011 250 µg $180 Human IgG Fc None Human IgG Fc specific polyclonal goat IgG Western blot G-102-C 100 µg $355

Human IgG1 Fc None Human IgG1 Fc specific monoclonal mouse IgG1 Western blot MAB110 500 µg $325 Human IgM None Human IgM specific polyclonal goat IgG Western blot G-105-C 100 µg $355 Human IgE None Human IgE specific polyclonal goat IgG Western blot G-107-C 100 µg $355 Human IgG (H+L) None Human IgG specific polyclonal goat IgG (H+L) chains Western blot G-101-C-ABS 500 µg $75

PerCP Mouse IgG specific polyclonal goat IgG F(ab')2 (H+L) Fragment Flow Cytometry F0114 100 Tests $155 None Mouse IgG specific polyclonal goat IgG Western blot AF007 500 µg $99 Biotin Mouse IgG specific polyclonal goat IgG Western blot BAF007 250 µg $100 Mouse IgG Biotin Mouse IgG specific polyclonal donkey IgG Western blot BAF018 250 µg $75 HRP Mouse IgG specific polyclonal goat IgG Western blot HAF007 1 mL $105 HRP Mouse IgG specific polyclonal donkey IgG Western blot HAF018 1 mL $95 Mouse IgG Fc None Mouse IgG Fc specific polyclonal goat IgG Western blot G-202-C 100 µg $355 Mouse IgG None Mouse IgG specific polyclonal donkey IgG Western blot D-201-C-ABS2 500 µg $75

APC Mouse IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment Flow Cytometry F0101B 100 Tests $175

Mouse IgG F(ab’)2 (H+L) Fluorescein Mouse IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment Flow Cytometry F0103B 100 Tests $105

PE Mouse IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment Flow Cytometry F0102B 100 Tests $135 None Rabbit IgG specific polyclonal goat IgG Western blot AF008 500 µg $75 Biotin Rabbit IgG specific polyclonal goat IgG Western blot BAF008 250 µg $75 HRP Rabbit IgG specific polyclonal goat IgG Western blot HAF008 1 mL $95 Rabbit IgG APC Rabbit IgG specific polyclonal goat IgG Flow Cytometry F0111 100 Tests $220 Fluorescein Rabbit IgG specific polyclonal goat IgG Flow Cytometry F0112 100 Tests $140 PE Rabbit IgG specific polyclonal goat IgG Flow Cytometry F0110 100 Tests $175 None Rabbit IgG specific polyclonal donkey IgG Western blot D-301-C-ABS2 500 µg $75 HRP Rat specific polyclonal goat IgG Western blot HAF005 1 mL $105 None Rat IgG specific polyclonal goat IgG Western blot AF005 500 µg $100 Rat IgG

PerCP Rat IgG specific polyclonal goat IgG F(ab')2 (H+L) Fragment Flow Cytometry F0115 100 Tests $190 Biotin Rat IgG specific polyclonal goat IgG Western blot BAF005 250 µg $100

Rat IgG F(ab’)2 APC Rat IgG specific polyclonal goat IgGF(ab’)2 (H+L) Fragment Flow Cytometry F0113 100 Tests $210

Fluorescein Rat IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment Flow Cytometry F0104B 100 Tests $120 Rat IgG F(ab’)2 (H+L) PE Rat IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment Flow Cytometry F0105B 100 Tests $175

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SECONDARY ANTIBODIES analyte label type applications catalog # size price Biotin Sheep IgG specific polyclonal donkey IgG Western blot BAF016 250 µg $75 Sheep IgG HRP Sheep IgG specific polyclonal donkey IgG Western blot HAF016 1 mL $95 None Sheep IgG specific polyclonal donkey IgG Western blot D-501-C-ABS 500 µg $75 APC Mouse IgM specific polyclonal goat IgG Flow Cytometry F0117 100 Tests $220 Fluorescein Mouse IgM specific polyclonal goat IgG Flow Cytometry F0118 100 Tests $140 Mouse IgM PerCP Mouse IgM specific polyclonal goat IgG Flow Cytometry F0119 100 Tests $190 PE Mouse IgM specific polyclonal goat IgG Flow Cytometry F0116 100 Tests $175

NorthernLights™ A line of fluorochrome-conjugated secondary reagents for IHC/ICC NorthernLights secondary antibodies are bright and fade-resistant, recognizing mouse, goat, sheep, rat, and rabbit IgG and chicken IgY. Like all R&D Systems antibodies, NorthernLights antibody conjugates are specific and deliver a high signal-to-noise ratio. Also available are streptavidin conjugates for labeling biotinylated antibodies.

description conjugate catalog # size price NorthernLights-557 NL001 500 µL $115 Donkey Anti-Goat IgG, Cross-adsorbed NorthernLights-637 NL002 500 µL $115 NorthernLights-493 NL003 500 µL $115 NorthernLights-557 NL004 500 µL $115 Donkey Anti-Rabbit IgG, Cross-adjusted NorthernLights-637 NL005 500 µL $115 NorthernLights-493 NL006 500 µL $115 NorthernLights-557 NL007 500 µL $115 Donkey Anti-Mouse IgG, Cross-adsorbed NorthernLights-637 NL008 500 µL $115 NorthernLights-493 NL009 500 µL $115 NorthernLights-557 NL010 500 µL $115 Donkey Anti-Sheep IgG, Cross-adsorbed NorthernLights-637 NL011 500 µL $115 NorthernLights-493 NL012 500 µL $115 NorthernLights-557 NL013 500 µL $115 Goat Anti-Rat IgG NorthernLights-637 NL014 500 µL $115 NorthernLights-493 NL015 500 µL $115 NorthernLights-557 NL016 500 µL $115 Goat Anti-Chicken IgY NorthernLights-637 NL017 500 µL $115 NorthernLights-493 NL018 500 µL $115 Streptavidin NL493 NorthernLights-493 NL997 500 µL $115 Streptavidin NL637 NorthernLights-637 NL998 500 µL $115 Streptavidin NL557 NorthernLights-557 NL999 500 µL $115

ANTIBODY CONTROLS control label type catalog # size price Fluorescein Normal goat IgG IC108F 200 Tests $165 PE Normal goat IgG IC108P 200 Tests $165 Goat IgG PerCP Normal goat IgG IC108C 200 Tests $165 None Normal goat IgG AB-108-C 1 mg $75 Biotin Normal goat IgG BAF108 100 µg $75

Human IgG1 Fc None NS0-expressed recombinant protein 110-HG-100 100 µg $325 Human IgG None Normal human IgG 1-001-A 1 mg $75 None Normal sheep IgG 5-001-A 1 mg $75 Sheep IgG APC Normal sheep IgG IC016A 200 Tests $165 Fluorescein Normal sheep IgG IC016F 200 Tests $165

110 For research use only. Not for use in diagnostic procedures. IHC PROTOCOL GUIDE

ANTIBODY CONTROLS, continued control label type catalog # size price

PerCP Normal mouse IgG1, clone # 11711 IC002C 200 Tests $165

APC Normal mouse IgG1, clone # 11711 IC002A 200 Tests $165

None Normal mouse IgG1, clone # 11711 MAB002 500 µg $170 Mouse IgG1 Biotin Normal mouse IgG1, clone # 11711 IC002B 200 Tests $165

Fluorescein Normal mouse IgG1, clone # 11711 IC002F 200 Tests $165

PE Normal mouse IgG1, clone # 11711 IC002P 200 Tests $165

None Normal mouse IgG2B, clone # 20102 MAB003 500 µg $170

None Normal mouse IgG2B, clone # 133304 MAB0031 500 µg $170

PerCP Normal mouse IgG2B, clone # 20102 IC003C 200 Tests $165

Mouse IgG2A APC Normal mouse IgG2B, clone # 20102 IC003A 200 Tests $165

Biotin Normal mouse IgG2B, clone # 20102 IC003B 200 Tests $165

Fluorescein Normal mouse IgG2B, clone # 20102 IC003F 200 Tests $165

PE Normal mouse IgG2B, clone # 20102 IC003P 200 Tests $165

Mouse IgG2A Fc None NS0-expressed recombinant protein 4460-MG-100 100 µg $325

None Normal mouse IgG2B, clone # 20116 MAB004 500 µg $170

None Normal mouse IgG2B, clone # 133303 MAB0041 500 µg $170

None Normal mouse IgG2BB, clone # 73009 MAB0042 500 µg $170

Mouse IgG2B APC Normal mouse IgG2B, clone # 133303 IC0041A 200 Tests $165

Fluorescein Normal mouse IgG2B, clone # 133303 IC0041F 200 Tests $165

PE Normal mouse IgG2B, clone # 133303 IC0041P 200 Tests $165

PerCP Normal mouse IgG2B, clone # 133303 IC0041C 200 Tests $165

None Normal mouse IgG3, clone # 133316 MAB007 500 µg $170

APC Normal Mouse IgG3, clone # 133316 IC007A 200 Tests $165 Mouse IgG3 Fluorescein Normal Mouse IgG3, clone # 133316 IC007F 200 Tests $165

PE Normal Mouse IgG3, clone # 133316 IC007P 200 Tests $165 APC Normal rabbit IgG IC105A 200 Tests $165 Fluorescein Normal rabbit IgG IC105F 200 Tests $165 Rabbit IgG PE Normal rabbit IgG IC105P 200 Tests $165 None Normal rabbit IgG AB-105-C 1 mg $75 Rat IgG None Normal rat IgG 6-001-A 1 mg $75

None Normal rat IgG1, clone # 43414 MAB005 500 µg $170

APC Normal rat IgG1, clone # 43414 IC005A 200 Tests $165

Rat IgG1 Biotin Normal rat IgG1, clone # 43414 IC005B 200 Tests $165

Fluorescein Normal rat IgG1, clone # 43414 IC005F 200 Tests $160

PE Normal rat IgG1, clone # 43414 IC005P 200 Tests $165

None Normal rat IgG2A, clone # 54447 MAB006 500 µg $170

APC Normal rat IgG2A, clone # 54447 IC006A 50 µg $165

Biotin Normal rat IgG2A, clone # 54447 IC006B 50 µg $165 Rat IgG2A Fluorescein Normal rat IgG2A, clone # 54447 IC006F 200 Tests $165

PE Normal rat IgG2A, clone # 54447 IC006P 200 Tests $165 PerCP Normal rat IgG, clone # 54447 IC006C 200 Tests $165 PerCP Normal rat IgG, clone # 141945 IC013C 200 Tests $165

APC Normal rat IgG2B, clone # 141945 IC013A 200 Tests $165

Biotin Normal rat IgG2B, clone # 141945 IC013B 200 Tests $165 Rat IgG2B Fluorescein Normal rat IgG2B, clone # 141945 IC013F 200 Tests $165

PE Normal rat IgG2B, clone # 141945 IC013P 200 Tests $165

None Normal rat IgG2B, clone # 141945 MAB0061 500 µg $170

Mouse IgG3 PerCP Normal mouse IgG, clone # 133316 IC007C 200 Tests $165 Mouse IgM PerCP Normal mouse IgM IC015C 200 Tests $165 Chicken IgY None Normal chicken IgY AB-101-C 1 mg $75

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Staining Kits & Reagents for Cells & Tissues Cell and Tissue Staining Kits are intended for localization of antigens in a broad range of histological and cytological specimens. These kits are based on the formation of the Avidin-Biotin Complex (ABC) with primary antibodies that react with the tissue antigens under study. The kits are made in ready-to-use format including prediluted Secondary Biotinylated Antibodies, and HSS-HRP that eliminates the need for extra steps in the procedure, mini- mizes hands-on time, maximizes convenience, and reduces the risk of erroneous calculations. Cell & Tissue Staining Kits species label components catalog # Size Price Anti-goat HRP-DAB System Secondary Biotinylated Antibody, Streptavidin-HRP Conjugate, DAB CTS008 50 Tests $240 Anti-mouse Chromogen, DAB Chromogen Buffer, Blocking Reagents CTS002 Anti-rabbit CTS005 Anti-rat CTS017 Anti-sheep CTS019 Anti-goat HRP-AEC System Secondary Biotinylated Antibody, Streptavidin-HRP Conjugate, AEC CTS009 50 Tests $240 Anti-mouse Chromogen, AEC Chromogen Buffer, Blocking Reagents CTS003 Anti-rabbit CTS006 Anti-rat CTS018 Anti-sheep CTS020

product description catalog # Size Price Includes reagents for antigen retrieval, blocking reagent, detection reagents, and a specific monoclonal p27/Kip1 IHC Detection Kit CTS2256 50 Tests $425 primary antibody for human p27 antigen

p27 in Human Prostate Cancer. p27 was detected in paraffin-embedded human prostate cancer sections using the Human p27 Immunohistochemistry Detec- tion Kit (Catalog # CTS2256). Tissue was stained using anti-mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).

Cell & Tissue Staining Reagents product description catalog # Size Price Basic Antigen Retrieval Reagent Antigen retrieval system that utilizes a heat-induced recovery of cell and tissue antigens. CTS013 50 mL $60 Acidic Antigen Retrieval Reagent Antigen retrieval system that utilizes a heat-induced recovery of cell and tissue antigens. CTS014 50 mL $60 Universal Antigen Retrieval Reagent Antigen retrieval system that utilizes a heat-induced recovery of cell and tissue antigens. CTS015 50 mL $60 Antigen Retrieval Reagent Sampler All three reagents (CTS013, CTS014, CTS015) in one convenient pack. CTS016 50 mL $120 Avidin-Fluorescein For use as a secondary reagent in immunofluorescent assays in conjunction with biotinylated F0030 100 Tests $65 primary labeling reagents. Streptavidin-Allophycocyanin For use as a secondary reagent in immunofluorescent assays in conjunction with biotinylated F0050 100 Tests $85 primary labeling reagents. Streptavidin-Phycoerythrin For use as a secondary reagent in immunofluorescent assays in conjunction with biotinylated F0040 100 Tests $75 primary labeling reagents. Streptavidin-Alkaline Phosphatase For use in ELISA or Western blot assays. AR001 1 mL $145 DAB Enhancer DAB Enhancer is a concentrated 50X metal based solution used to intensify the DABChro- CTS010 3 mL $45 mogen (Catalog Numbers # CTS002, CTS005, CTS008, CTS017). In the presence of the DAB Enhancer, the DAB Chromogen produces a clear brown-black staining with minimal background. Mounting Medium Permanent aqueous mounting medium CTS011 15 mL $35

Control Antigen Retrieval Antigen Retrieval Improves Detection of Neuro- plastin 65. Neuroplastin 65 was detected in human cerebellar tissue using anti-human/mouse Neuro- plastin 65 affinity-purified polyclonal antibody (Cat- alog # AF5360). Tissue was pretreated with Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the anti-Goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

112 For research use only. Not for use in diagnostic procedures. Ordering Information

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USA & Canada R&D Systems, Inc. Europe R&D Systems Europe Ltd. China R&D Systems China Co., Ltd. 614 McKinley Place NE 19 Barton Lane, Abingdon Science Park 24A1 Hua Min Empire Plaza Minneapolis, MN 55413, USA Abingdon OX14 3NB, UK 726 West Yan An Road, Shanghai, PRC 200050 TEL: (800) 343-7475 (612) 379-2956 TEL: +44 (0)1235 529449 TEL: +86 (21) 52380373 FAX: (612) 656-4400 FAX: +44 (0)1235 533420 FAX: +86 (21) 52371001 E-MAIL: [email protected] E-MAIL: [email protected] E-MAIL: [email protected] www.RnDSystems.com www. RnDSystems.com www.RnDSystemsChina.com.cn

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