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AP32202PU-N Polyclonal Antibody to APG4D / ATG4D (227-257) - Purified

Alternate names: AUT-like 4 cysteine endopeptidase, AUTL4, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related 4 homolog D, Cysteine protease ATG4D Quantity: 0.4 ml Concentration: lot specific Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Uniprot ID: Q86TL0 NCBI: NP_116274.3 GeneID: 84971 Host / Isotype: Rabbit / Ig Immunogen: KLH conjugated synthetic peptide between 227~257 amino acids from the Center of Human APG4D Genename: ATG4D Format: State: Liquid purified Ig fraction Purification: Saturated Ammonium Sulfate precipitation followed by dialysis against PBS Buffer System: PBS containing 0.09% (W/V) Sodium Azide as preservative Applications: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry on Paraffin Sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Molecular Weight: 52922 Da Specificity: This antibody recognizes Human and Mouse ATG4D (227-257). Other species not tested.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 1 / 3 AP32202PU-N: Polyclonal Antibody to APG4D / ATG4D (227-257) - Purified

Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) 2. Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) 3. Greenberg JT. Dev Cell. 8(6):799-801. (2005) 4. Levine B. Cell. 120(2):159-62. (2005) 5. Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) Pictures: Formalin-fixed and paraffin-embedded human tissue reacted with the primary antibody, which was peroxidase- conjugated to the secondary antibody, followed by AEC staining.

Western blot analysis of ATG4D Antibody Cat.-No AP32202PU-N in 293 cell line lysates transiently transfected with the APG4D (2ug/lane). hAPG4D- L235(arrow) was detected using the purified Pab (1:60 dilution).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 2 / 3 AP32202PU-N: Polyclonal Antibody to APG4D / ATG4D (227-257) - Purified

Western blot analysis of ATG4D Antibody Cat.-No AP32202PU-N in HL-60 cell line lysate.

Western blot analysis of ATG4D Antibody Cat.-No AP32202PU-N in mouse kidney tissue lysate.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 3 / 3

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