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Rnascope® Probes for Viral Pathogens Get Probes for Your Virus of Interest Designed and Manufactured in Two Weeks Probe List Series Viral Pathogens RNAscope® Probes for Viral Pathogens Get probes for your virus of interest designed and manufactured in two weeks Featured Publications using RNAscope® Probes for Detection of Viral RNA RNAscope® Technology Infectious diseases have a devastating effect on worldwide public health and mortality. With recent CD8(+) Lymphocytes Are Required for outbreaks of Zika and Ebola viruses, early and precise detection of viral pathogens is critical for treatment Maintaining Viral Suppression in SIV- Infected Macaques Treated with Short- and containment. RNA in situ hybridization (ISH) has been instrumental in the detection of viral RNA in tumor Term Antiretroviral Therapy. Cartwright samples, including human papilloma virus (HPV) and Epstein-Barr virus (EBV). Overall, the detection of viral EK, et. al. Immunity 2016 Sep PMID: pathogens by RNA ISH can be a powerful tool to establish the etiology of pathogenic diseases in various hosts. 27653601 The RNAscope® in situ hybridization technology provides single-cell gene expression resolution with spatial Zika Virus Infection in Mice Causes and morphological context, ideal for viral detection applications. Panuveitis with Shedding of Virus in Tears. Miner JJ, et. al. Cell Reports • Single-molecule sensitivity enables detection of individual viral particles even in low viral loads 2016 Sep PMID: 27612415 • High specificity delivers accurate detection among highly homologous species or strains Defining HIV and SIV Reservoirs in • Speed of workflow and probe design make RNAscope® ISH a responsive solution for emergent Lymphoid Tissues. Deleage C, et. al. or novel viruses Pathog Immun 2016 PMID: 27430032 • Viral localization enables elucidation of transmission, pathogen-host interactions, hidden reservoirs, etc. Specific Detection of Two Divergent Simian Arteriviruses Using RNAscope • Differential detection targeting the sense or anti-sense strand enables differentiation of viral stages In Situ Hybridization. Yú SQ, et. al. 2016 Mar PLoS One PMID: 26963736 ® Localization of Bovine Papillomavirus RNAscope probes can be designed for any type of virus Nucleic Acid in Equine Sarcoids. Gaynor AM, et. al. Vet Pathol. 2016 DNA Viruses RNA Viruses Retro-Transcribing Viruses May PMID: 26215759 ds DNA ss DNA ds RNA ss RNA ss RNA ss RNA ds DNA RNAscope® ISH has been featured in I II III IV V VI VII over 140 virology publications acdbio.com/publications DNA (+/-) DNA (+) RNA (+/-) RNA (+) RNA (-) RNA (+) DNA (+/-) RNA (-) Reverse Reverse Transcription Transcription DNA (+/-) DNA (+/-) DNA (+/-) mRNA Proteins Visualize, localize and quantify viral RNA today acdbio.com/viralpathogens DETECTION OF HUMAN PAPILLOMAVIRUS (HPV) IN HUMAN CANCER SAMPLES USING RNASCOPE® 2.0 OR 2.5 HD ASSAY–BROWN V-HPV-HR7 V-HPV-E6-E7 Head and neck cancer Head and neck cancer V-HPV-HR18 V-HPV-HR18 Head and neck cancer Cervical cancer DETECTION OF CYTOMEGALOVIRUS (CMV) IN HUMAN LIVER AND KIDNEY TISSUES USING RNASCOPE® 2.5 HD ASSAY–BROWN V-CMV V-CMV Liver Kidney 2 Probe List Series DETECTION OF EPSTEIN-BARR VIRUS (EBV) IN HUMAN CANCER SAMPLES USING RNASCOPE® 2.5 HD ASSAY–BROWN V-EBV-EBER1 V-EBV-EBER1 Lymphoma Nasopharyngeal carcinoma DETECTION OF ZIKA VIRUS (ZIKV) SOLELY IN INFECTED MOUSE SAMPLES USING RNASCOPE® 2.5 HD ASSAY-RED. NO SIGNALS SEEN IN UNINFECTED SAMPLES. V-ZIKA-pp V-ZIKA-pp Mouse infected brain Mouse infected kidney V-ZIKA-pp V-ZIKA-pp Mouse uninfected brain Mouse uninfected kidney Viral Pathogens 3 RNAscope® Probes for Viral Pathogens Multiple probes are available for each viral species listed below. Read more at acdbio.com/viralpathogens Adeno-associated virus Frog virus Lymphocytic choriomeningitis Rodent hepacivirus virus Alpaca polyomavirus Hedgehog hepatovirus Rodent hepatovirus Macacine herpesvirus Andes virus Hendra virus Rotatvirus Marburg virus Avian infectious bronchitis virus Hepatitis A virus Rubella virus Measles virus Bat hepadnavirus Hepatitis B virus SARS coronavirus Merkel cell polyomavirus Bat hepatovirus Hepatitis C virus Self-complementary Middle East respiratory adeno-associated virus Bovine astrovirus Hepatitis D virus syndrome coronavirus Seneca valley virus Bovine papilloma virus Hepatitis E virus Mumps virus Severe fever with Bovine parvovirus Hepatitis GB virus Murine herpesvirus thrombocytopenia Canine bocavirus Human betacoronavirus syndrome virus Mus musculus papillomavirus Canine herpesvirus Human bocavirus Shrew hepadnavirus Newcastle disease virus Canine papilloma virus Human coronavirus Shrew hepatovirus Nipah virus Canine parvovirus Human coxsackievirus Simian hemorrhagic fever virus Norovirus Cercopithecine herpes virus Human endogenous retrovirus Simian immunodeficiency virus Ovine herpesvirus Chikungunya virus Human herpesvirus Simian parvovirus Parechovirus Circovirus Human immunodeficiency Simian virus 40 Parvo-like virus virus 1 Citrus tristeza virus Sin Nombre hantavirus Phocoenid herpesvirus Human papillomavirus Cynomolgus macaque Theiler’s disease- Porcine astrovirus cytomegalovirus Human parechovirus associated virus Porcine parainfluenza virus Dengue virus Human parvovirus Vaccinia virus Powassan virus Eastern equine Human rhinovirus Venezuelan equine encephalitis virus Rabies virus Human T-lymphotropic virus 1 encephalitis virus Enterovirus Raccoon polyomavirus Influenza virus Vesicular stomatitis Equid herpesvirus Respiratory syncytial virus Indiana virus Japanese encephalitis virus Equine arteritis virus Retroperitoneal fibromatosis- Yellow fever virus Kirkovirus associated herpesvirus Felid herpesvirus Zaire ebolavirus Langat virus Rhesus cytomegalovirus Feline immunodeficiency virus Zika virus Lassa virus Feline infectious peritonitis virus Feline papillomavirus If probes for your virus of interest is not yet available, RNAscope® Made-to-Order Target Probes can be designed and manufactured within 2 weeks for visualization of virtually ANY gene of ANY viral species in ANY infected tissue. Learn more at acdbio.com/target-probes-made-order Visualize, localize and quantify viral RNA today acdbio.com/viralpathogens For Research Use Only. Not for diagnostic use. RNAscope is a registered trademark of Advanced Cell Diagnostics, Inc. in the United States or other countries. All rights reserved. ©2016 Advanced Cell Diagnostics, Inc. Doc #: MK 51-082/Rev A/Effective date 013017 California, USA.
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