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FIRST RECORD FOR TWO FROM ARGENTINA: australis DATOVO & DE PINNA, 2014 AND macanuda FERRER & MALABARBA, 2020 (SILURIFORMES: )

Primeros registros de dos bagres para Argentina: Ituglanis australis Datovo & de Pinna, 2014 y Scleronema macanuda Ferrer & Malabarba, 2020 (Siluriformes: Trichomycteridae)

Guillermo Enrique Terán1, Sergio Bogan2, Stefan Koerber3 y Juliano Ferrer4*

1Fundación Miguel Lillo - UEL CONICET. Miguel Lillo 251, 4000 Tucumán, Argentina 2Fundación de Historia Natural “Félix de Azara”- Centro de Ciencias Naturales, Ambientales y Antropológicas, Universidad Maimónides, Ciudad Autónoma de Buenos Aires, Argentina. 3Independent researcher. Friesenstr. 11, 45476 Muelheim, Germany. www.pecescriollos.de 4Programa de Pós-Graduação em Biologia , Departamento de Zoologia, Universidade Federal do , Av. Bento Gonçalves, 9500, Agronomia, 91501-970, Porto Alegre, RS, . *[email protected]

39 Terán g., Bogan s., Koerber s. y Ferrer j.

Abstract. Ituglanis australis and Scleronema macanuda are trichomycterid catfishes that inhabit freshwater basins from Pampa grasslands of southern Brazil and . The aim of the present contribution is to report for the first time the presence of both in Argentina. These new records are related to small streams of the Uruguay River basin in Entre Ríos province. Both were identified based on ichthyological collections that were made before they were formally described. With these reports the number of Argentinian trichomycterids is increasing to 42.

Key words. Entre Ríos, Trichomycteridae, Pampa grasslands, Ituglanis, Scleronema.

Resumen. Ituglanis australis y Scleronema macanuda, son bagres tricomictéridos que habitan en las cuencas de la región de Pampa y Pastizales del sur de Brasil y Uruguay. En este trabajo documentamos por primera vez la presencia de estas especies en Argentina. Estos nuevos registros están vinculados a pequeños arroyos que forman parte de la cuenca del río Uruguay en la provincia de Entre Ríos y se lograron identificar gracias a la revisión de colecciones ictiológicas formadas años antes de que estas especies fueran descriptas formalmente. Los dos registros incrementan a 42 el número de especies de tricomictéridos documentados en la Argentina.

Palabras clave. Entre Ríos, Trichomycteridae, Pampa y Pastizales, Ituglanis, Scleronema.

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INTRODUCTION located, with the medial margins of the autopalatines very close to each other; and Trichomycteridae is the second most autopalatine with an interrupted or not in- species-rich family of Siluriformes com- terrupted ossified arch-shaped process on prising more than 330 valid species (Fricke its dorsal surface forming a canal (Ferrer & et al., 2020) widespread in the Neotropical Malabarba, 2020). Scleronema macanuda Fer- region from Costa Rica to Argentina. Itug- rer & Malabarba, 2020, is the largest spe- lanis and Scleronema are included in the cies of the (reaching 15 cm of total subfamily and have been length), being found associated to sandy demonstrated as monophyletic on the basis bottoms of rivers and streams in the Atlan- of morphological and molecular hypoth- tic coastal drainages and the lower Uru- eses (Costa and Bockmann, 1993; de Pinna, guay River basin in Southern Brazil and 1998; Ferrer, 2016; Ferrer & Malabarba, 2020; Uruguay (Ferrer & Malabarba, 2020). Ochoa et al., 2017; 2020). Herein, we report the first records ofItug - Currently, Ituglanis consists of 29 small- lanis australis and Scleronema macanuda for sized species with occurrence in the main both the province of Entre Ríos and Argen- hydrographic systems of tina, on the basis of a review of local and (Fricke et al., 2020). According to Costa & foreign Fish Collections. Bockmann (1993), three synapomorphies are shared by the species of Ituglanis: pos- terior cranial fontanel present as an orifice MATERIAL AND METHODS situated posteriorly in the parieto-supraoc- cipital; anterior portion of the sphenotic- Two trichomycterid specimens, KU 19260 prootic-pterosphenoid directed anteriorly; and CFA-IC-6507, were analyzed, housed and presence of a deep concavity in the at the University of Kansas Natural History medial margin of the autopalatine. Ituglanis Museum and Biodiversity Research Center australis Datovo & de Pinna, 2014 presents (KU; USA) and Colección Ictiológica de la the southernmost distribution within the Fundación Félix Azara (CFA-IC; Argentina) genus, inhabiting the lower Uruguay River respectively. Identifications were conducted basin and the Patos-Merín system in South based on the taxonomic studies of Datovo Brazil and Uruguay (Datovo & de Pinna, & de Pinna (2014) and Ferrer & Malabarba 2014). In the Negro River basin, specimens (2020). In order to facilitate the comparison, of I. australis are usually found in small all measurements, counts, and the nomen- streams associated with submerged roots clature of the laterosensory canal system or bottom covered with leaf litter (Serra et and associated pores follow these two pa- al., 2014). pers. An x-ray was performed for the lot KU Scleronema contains eight species that 19260 at the Museum Support Center of the inhabit the Pampa grasslands in Argen- Smithsonian Institution (USA). tina, Brazil, Paraguay, and Uruguay. A systematic revision of Scleronema was re- cently conducted by Ferrer and Malabarba RESULTS (2020) resulting in four synapomorphies for the genus: fleshy flap at the base of the Ituglanis australis Datovo & de Pinna, 2014 maxillary barbel; skin flap in the posterior Figures 1-3 and Table 1 margin of the opercle; articulation between Examined material from Argentina. KU the autopalatine and the vomer ventrally 19260, 1 ex., 81.9 mm SL, Ñancay River,

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(ca. 33°23’2.486’’S 58°44’30.235’’W) lower Ríos, Argentina (Figure 4). Coll: S. Menu- Uruguay River basin, province of Entre Marque, May 1980.

Figure 1 - Lateral and dorsal views of Ituglanis australis, KU 19260, 81.9 mm SL, Ñancay River, lower Uruguay River, province of Entre Ríos, Argentina.

Figure 2 - X-ray from lateral view of Ituglanis australis, KU 19260, 81.9 mm SL, Ñancay River, lower Uruguay River, province of Entre Ríos, Argentina.

Figure 3 - Specimen in life of Ituglanis australis (after capture, in aquarium, not preserved), Perucho Stream, lower Uruguay River, province of Entre Ríos, Argentina. Photograph taken by Nachobarragan, Estudiocreativa.

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Table 1 - Morphometric data and counts of Ituglanis australis (KU 19260).

Standard length (mm) 81.9 Counts Percent of standard length Pectoral-fin rays i+5 Total length 117.0 Dorsal-fin rays ii+7 Predorsal length 74.3 Procurrent rays of dorsal fin 3 Preanal length 75.4 Pterygiophores of dorsal fin 8 Prepelvic length 63.4 anterior to neural First pteryiogphore of dorsal-fin insertion spine of the 24th Caudal peduncule length 15.9 vertebra Body depth 15.5 Anal-fin rays ii+5 Caudal peduncule depth 12.1 Procurrent rays of anal fin 3 Dorsal-fin base length 11.1 Pterygiophores of anal fin 6 Anal-fin base length 9.6 anterior to haemal Head length 17.7 First pteryiogphore of anal-fin insertion spine of the 24th vertebra Percent of head length Pelvic-fin rays i+4 Head depth 49.7 Caudal-fin rays i+5+6+i Head width 94.1 Dorsal procurrent rays of caudal fin 12 Interorbital width 27.6 Ventral procurrent rays of caudal fin 13 Eye diameter 9.8 Vertebrae 38 Snout length 34.8 Ribs 6 Nasal barbel length 52.1 Breadth of mouth 35.6

Figure 4 - Geographical distribution of Ituglanis australis (yellow symbols) and Scleronema ma- canuda (white symbols) according to Datovo & de Pinna (2014), Serra et al. (2014), Ferrer & Ma- labarba (2020), and samples currently examined. Some dots may represent more than one locality. Squares indicate the new records for Argentina.

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Scleronema macanuda Ferrer & Malabarba, at road RN 14 (32°03’41.8’’S 58°15’40.14’’W), 2020 lower Uruguay River basin, Colón, province Figures 5-6 and Table 2 of Entre Ríos, Argentina (Figure 4). Coll: A. Material examined from Argentina. CFA- Miquelarena, H. López, L. Protogino & E. IC-6507, 1 ex., 53.3 mm SL, El Mármol Stream Etcheverry, 27 Nov 2006.

Figure 5 - Lateral (A, C) and dorsal (B) views of Scleronema macanuda, CFA-IC-6507, 53.3 mm SL, El Mármol Stream at road RN 14, lower Uruguay River basin, Colón, province of Entre Ríos, Argentina.

Figure 6 - Dorsal view of the head of Scleronema macanuda, CFA-IC-6507, 53.3 mm SL, showing the pores of the supraorbital and infraorbital branches of the laterosensory system (s1, s2, s3, s6, i10 and i11) and the skin flap in the pos- terior margin of the opercle (sfo).

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Table 2 - Morphometric data of Scleronema macanuda DISCUSSION (CFA-IC-6507). An updated list of the freshwater fishes Standard length (mm) 53.3 from Argentina was published by Mirande Percent of standard length & Koerber (2020) who listed 40 species of Total length 117.3 Trichomycteridae. The records of Itugla- Head length 18.8 nis australis and Scleronema macanuda, both Predorsal length 58.2 from tributaries of the lower Uruguay Riv- er basin in the Entre Ríos province, increase Prepelvic length 48.8 the number of trichomycterids found in the Preanal length 67.5 country to 42. Scapular girdle width 17.8 Ituglanis eichhorniarum was the single Trunk length 35.6 species of the genus recorded for Argen- Pectoral-fin length 16.9 tina, which has its distribution restricted Pelvic-fin length 15.0 to the Paraná River in Misiones and Cor- Distance between pelvic-fin base and anus 11.3 rientes Provinces (Azpelicueta and García, 1994; Miquelarena and Fernández, 2000). Caudal peduncule length 24.4 The lot of Ituglanis australis herein ana- Caudal peduncule depth 11.3 lyzed (KU 19260) was previously cited as Body depth 18.8 “Scleronema sp.” by Arratia (1998). At that Body width 9.4 time, the species had not been described Length of dorsal-fin base 15.0 yet and several traits observed in the speci- Length of anal-fin base 6.6 men agree with the diagnostic characters Percent of head length provided by Datovo & de Pinna (2014) for Head depth 60.0 I. australis: the dark brown stripes running along the lateral surface of body (Figure Nasal barbel length 20.0 1), i+5 pectoral fin rays (Table 1); pres- Maxillary barbel length 50.0 ence of the pores i1 and i3 of the infraor- Rictal barbel length 32.0 bital branch of the cephalic laterosensory Snout length 36.0 system; and eight pterygiophores in the Interorbital length 17.0 dorsal fin (Figure 2). Additionally, an- Mouth width 48.0 other specimen of I. australis collected and Eye diameter 10.0 released in the Perucho Stream, another tributary of the lower Uruguay River in Distance between snout tip to posterior nare 22.0 Argentina, could be identified recently in Intranarial length 8.0 a photograph (Figure 3). Anterior internarial width 11.0 Ferrer and Malabarba (2020) conducted a Posterior internarial width 10.0 systematic revision of the genus Scleronema Supraorbital pore s6 distance 8.0 that confirmed the presence of S. minutum Counts and the newly described S. milonga in Ar- Pectoral-fin rays i+6 gentina, but considered S. opercularum being restricted to the Ibicuí River, Rio Grande do Dorsal-fin rays ii+11 Sul State, Southern Brazil. In contrast, Sclero- Anal-fin rays i+6 nema macanuda, sister species of S. opercula- Pelvic-fin rays i+4 tum, has a wider distribution with occur- Caudal-fin rays i+5+5+i rence in the coastal drainages as well as in

HISTORIA NATURAL Tercera Serie Volumen 10 (3) 2020/39-47 HISTORIA NATURAL Tercera Serie Volumen 10 (3) 2020/39-47 45 Terán g., Bogan s., Koerber s. y Ferrer j. the lower Uruguay River basin in South Bra- Salso Stream, lower Uruguay River basin, zil and Uruguay (Figure 4). Santana do Livramento, Brazil. UFRGS The following diagnostic characters cited 26992, 2, 44.0-51.7 mm SL, stream tributary by Ferrer and Malabarba (2020) to diag- of Jaguarão River, Patos-Merín system, nose S. macunuda were easily observed in Candiota, Brazil. UFRGS 27006, 2, 63.1- the specimen from El Mármol Stream in 63.3 mm SL, stream tributary of Jaguarão Argentina: compressed body with its later- River, Patos-Merín system, Candiota, Bra- al surface composed of a midlateral series zil. Scleronema macanuda: see Ferrer and of 6-9 rounded black blotches larger than Malabarba (2020). opercle; maxillary barbel smaller than half- length of the head and with thinner portion shorter than wider; tip of maxillary barbel ACKNOWLEDGEMENTS extending between anterior and posterior margins of interopercle; fleshy flap at the Thank are due to the late Richard Vari (in base of the maxillary barbel located ante- memoriam) and to Sandra Raredon (USNM) riorly, thick, prolonged up to the snout for their support to JF in the Smithsonian and with distal margin straight; skin flap Institution. Leo Smith and Gloria Arratia posterior to opercle pointed and long; and (KU) for the loan of the Ituglanis australis lot. caudal fin with a vertical black bar distally Thanks to Adrián Giacchino, Juan Manuel (Figs. 5-6). Additionally, the laterosensory Meluso (Fundación de Historia Natural “Fé- system pattern described to S. macanuda by lix de Azara”- Universidad Maimónides), Ferrer & Malabarba (2020) is identical to Pablo Pereira (Fundación Miguel Lillo) and the specimen herein analyzed (Figure 6). Marcos Waldbillig for their support during The findings of both, Ituglanis australis the confection of this contribution. We thank and Scleronema macanuda, in the Argen- Estudiocreativa for figure 3. tinean province of Entre Ríos are not sur- prising due to their presence in the eastern tributaries of the lower Uruguay River in REFERENCES South Brazil and Uruguay. However, these two records reinforce the importance of the Arratia G. (1998) , a new genus of tricho- scientific collections to access biodiversity mycterid catfishes from the Argentinian Andes, with redescription of nigricans. Ich- and the taxonomic works as reliable source thyological Exploration of Freshwaters, 9(4), 347-70. of information to validate the avalible data. Costa, W.J.E.M. and Bockmann, F.A. (1993). Un nou- veau genre néotropical de la famille des Tricho- Comparative material examined. Itugla- mycteridae (Siluriformes: Loricarioidei). Revue nis australis: UFRGS 7444, 1, paratype, française d’aquariologie (Nancy), 20(2), 43-46. Datovo, A. and de Pinna, M.C.C. (2014). A new spe- 63.0 mm SL, Maestre de Campo Stream, cies of Ituglanis representing the southernmost lower Uruguay River basin, Durazno, Uru- record of the genus, with comments on phylo- guay. UFRGS 12578, 1, paratype, 40.4 mm genetic relationships (Teleostei: Siluriformes: SL, Pinto Stream, Patos-Merín system, São Trichomycteridae). Journal of Fish Biology, 84(2), Lourenço do Sul, Brazil. UFRGS 13600, 1, 314-327. Ferrer J. (2016) Filogenia e revisão taxonômica do paratype, 75.6 mm SL, Santa Isabel Stream, gênero Scleronema (Siluriformes: Trichomycteri- Patos-Merín system, Cristal, Brazil. UFRGS dae). [PhD Thesis]. Porto Alegre: Universidade 15687, 1, paratype, 62.2 mm SL, Jaguarão Federal do Rio Grande do Sul. River, lower Uruguay River basin, Can- Ferrer, J. and Malabarba, L.R. (2020) Systematic revi- diota, Brazil. UFRGS 19656, 1, 51.3 mm SL, sion of the Neotropical genus Scleronema

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(Siluriformes: Trichomycteridae), with descrip- Trichomycteridae (Teleostei: Ostariophysi: Si- tions of six new species from Pampa grasslands. luriformes) supporting a monophyletic Tricho- Neotropical Ichthyology, 18(2): e190081. mycterinae. Molecular phylogenetics and evolution, Fricke, R.; Eschmeyer, W.N. and Van der Laan, R. 115, 71-81. (2020). Eschmeyer’s Catalog of Fishes: Genera, Ochoa, L.E.; Datovo, A.; Do Nascimiento, C.; Roxo, Species, References. F.F.; Sabaj, M.H.; Chang, J. and Oliveira, C. (http://researcharchive.calacademy.org/re- (2020). Phylogenomic analysis of trichomycterid search/ichthyology/catalog/fishcatmain. catfishes (Teleostei: Siluriformes) inferred from asp). Electronic version accessed July 2020. ultraconserved elements. Scientific reports, 10(1), Miquelarena, A.M., and Fernández, L.A. (2000) Pres- 1-15. encia de Trichomycterus davisi (Haseman, 1911) de Pinna M.C.C. (1998) Phylogenetic relationships en la cuenca del Alto Paraná misionero (Siluri- of Neotropical Siluriformes (Teleostei: Ostari- formes: Trichomycteridae). Revista de Ictiología, ophysi): historical overview and synthesis of 8:41-45. hypotheses. En: L.R. Malabarba, R.E. Reis, R.P. Mirande, J.M. and Koerber, S. (2020): Checklist of Vari, Z.M.S. Lucena, C.A.S Lucena CAS (Eds.), the Freshwater Fishes of Argentina. 2nd edition. Phylogeny and classification of Neotropical fishes (pp. (CLOFFAR-2). Ichthyological Contributions of Peces 279-330). Porto Alegre: Edipucrs. Criollos, 72, 1-81. Serra, W.; Bessonart, J.; Teixeira de Mello, F.; Duarte, Ochoa L.E.; Roxo, F.F.; DoNascimiento. C.; Sabaj. A.; Malabarba, L. y Loureiro, M. (2014). Peces del M.H.; Datovo. A.; Alfaro. M.; and Oliveira, C. Río Negro Uruguay. MGAP-DINARA, Montevi- (2017) Multilocus analysis of the catfish family deo. 208 pp.

Recibido: 04/08/2020 - Aceptado: 25/09/2020 - Publicado: 15/12/2020

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