bioRxiv preprint doi: https://doi.org/10.1101/834861; this version posted November 8, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Depth-discrete eco-genomics of Lake Tanganyika reveals roles of diverse microbes, 2 including candidate phyla, in tropical freshwater nutrient cycling 3 4 Patricia Q. Tran1,2, Peter B. McIntyre3, Benjamin M. Kraemer4, Yvonne Vadeboncoeur5, Ismael 5 A. Kimirei6, Rashid Tamatamah6, Katherine D. McMahon1,7, Karthik Anantharaman1 6 1. Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA 7 2. Department of Integrative Biology, University of Wisconsin-Madison, Madison, WI, 8 USA 9 3. Department of Ecology and Evolution, Cornell University, Ithaca, NY, USA 10 4. Department of Ecosystem Research, Leibniz Institute for Freshwater Ecology and Inland 11 Fisheries, Berlin, Germany 12 5. Department of Biological Sciences, Wright State University, Dayton, OH, USA 13 6. Tanzania Fisheries Research Institute (TAFIRI), Dar es Salaam, Tanzania 14 7. Department of Civil and Environmental Engineering, University of Wisconsin-Madison, 15 Madison, WI, USA 16 17 Corresponding author: Karthik Anantharaman (
[email protected]) 18 19 Abstract 20 Lake Tanganyika, the largest tropical freshwater lake and the second largest by volume 21 on Earth is characterized by strong oxygen and redox gradients. In spite of the majority of its 22 water column being anoxic, Tanganyika hosts some of the most diverse and prolific fisheries and 23 ecosystems on Earth.