USP10 Antibody Cat. No.: 5803

Western blot analysis of USP10 in Jurkat cell lysate with USP10 antibody at (A) 1 and (B) 2 μg/mL.

Immunofluorescence of USP10 in Jurkat cells with USP10 Immunocytochemistry of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL. antibody at 20 μg/mL.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse

Predicted species reactivity based on immunogen sequence: Bovine: (80%), Rat: (91%), HOMOLOGY: Chicken: (89%)

USP10 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human USP10. IMMUNOGEN:

The immunogen is located within amino acids 30 - 80 of USP10.

TESTED APPLICATIONS: IF

September 25, 2021 1 https://www.prosci-inc.com/usp10-antibody-5803.html USP10 antibody can be used for detection of USP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate

2) Cat. No. 17-005 - Jurkat Cell Slide

Properties

PURIFICATION: USP10 Antibody is affinity chromatography purified via peptide column.

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: USP10 Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION: 1 mg/mL

USP10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: USP10

USP10 Antibody: UBPO, KIAA0190, carboxyl-terminal hydrolase 10, ALTERNATE NAMES: 10

ACCESSION NO.: NP_005144

PROTEIN GI NO.: 119220605

GENE ID: 9100

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 25, 2021 2 https://www.prosci-inc.com/usp10-antibody-5803.html USP10 Antibody: USP10, also known as ubiquitin specific peptidase 10, belongs to the ubiquitin-specific protease family of cysteine proteases. USP10 functions to catalyze the cleavage of ubiquitin from ubiquitin-conjugated protein substrates such as p53/TP53, BACKGROUND: SNX3 and CFTR. USP10 has been identified as a subunit of DNA-bound (AR) complexes and may play a role in the activity of the DNA-bound androgen receptor complex. USP10 also acts as an essential regulator of p53/TP53 stability and is thought to function as a tumor suppressor.

1) D’Andrea A and Pellman D. Deubiquitinating enzymes: a new class of biological REFERENCES: regulators. Crit. Rev. Biochem. Mol. Biol.1998; 33:337-52.

2) Bomberger JM, Barnaby RL, and Stanton BA. The deubiquitinating enzyme USP10 regulates the endocytic recycling of CFTR in airway epithelial cells. Channels2010; 4(3).

3) Faus H, Meyer HA, Huber M, et al. The ubiquitin-specific protease USP10 modulates androgen receptor function. Mol. Cell. Endocrinol.2005; 245:138-46.

4) Yuan J, Luo K, Zhang L, et al. USP10 regulates p53 localization and stability by deubiquitinating p53. Cell 2010; 140:384-96.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 25, 2021 3 https://www.prosci-inc.com/usp10-antibody-5803.html