DDX31 (NM 138620) Human Tagged ORF Clone – RC221612 | Origene

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC221612

DDX31 (NM_138620) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: DDX31 (NM_138620) Human Tagged ORF Clone Tag: Myc-DDK Symbol: DDX31 Synonyms: PPP1R25 Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 4 DDX31 (NM_138620) Human Tagged ORF Clone – RC221612

ORF Nucleotide >RC221612 representing NM_138620 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGGCGCCGGATCTCGCTTCTCAGAGGCATTCAGAGAGCTTCCCCAGCGTGAACTCTCGGCCGAATGTAA TTTTGCCAGGCCGCGAGGGACGCAGAGAGGGGCTGCCACCAGGCGGGGGCACGCGCGGTAGTCTTGTCCC TACCCGCCCGGTGCCGCCGTCCCCGGCTCCCCTCGGGACGAGTCCGTACAGCTGGAGCCGTTCCGGACCC GGAAGGGGCGGTGGCGCAGGGTCCTCGCGCGTCCCACGTGGGGTCCCGGGCCCAGCAGTCTGCGCACCGG GCTCGCTGCTGCACCACGCGTCACCCACGCAGACCATGGCAGCCGCCGACGGTTCGCTCTTCGACAACCC CAGGACGTTCTCCAGACGTCCCCCAGCCCAGGCGAGTCGGCAAGCAAAGGCTACGAAAAGAAAATACCAA GCGTCCAGTGAGGCTCCCCCAGCGAAACGGAGGAACGAAACTTCATTTCTCCCAGCCAAGAAAACTAGTG TTAAAGAAACTCAGAGGACTTTTAAGGGGAACGCACAAAAAATGTTTTCTCCAAAGAAGCATTCGGTTAG CACAAGTGATAGAAACCAGGAGGAGAGACAGTGCATTAAGACTTCATCACTGTTTAAAAACAACCCTGAC ATTCCAGAACTCCACAGACCTGTGGTAAAGCAGGTGCAAGAAAAAGTGTTTACTTCAGCTGCTTTTCATG AGCTGGGCCTCCACCCACATTTAATTTCCACAATAAATACGGTCTTAAAAATGTCTAGTATGACCAGTGT TCAGAAGCAAAGTATTCCTGTGTTGCTGGAAGGCAGAGATGCTCTCGTGAGATCCCAGACGGGCTCAGGT AAAACTCTTGCCTATTGCATCCCTGTGGTCCAGTCCCTTCAAGCAATGGAGTCAAAAATACAGCGCAGTG ATGGCCCCTATGCCCTGGTGCTCGTGCCAACGAGAGAGCTAGCTCTACAAAGCTTTGACACTGTCCAGAA ACTGCTTAAGCCTTTCACCTGGATTGTGCCTGGAGTGTTAATGGGAGGAGAGAAGAGAAAATCAGAAAAG GCCAGACTCCGCAAAGGAATAAATATCCTTATCTCAACTCCTGGACGCCTGGTGGATCATATAAAATCCA CAAAGAACATTCATTTTAGTCGGCTGCGGTGGTTGGTGTTTGATGAAGCAGACAGAATCTTGGATTTGGG TTTTGAAAAGGACATCACAGTGATACTTAATGCTGTAAATGCTGAATGCCAAAAACGACAGAATGTCTTG CTATCAGCGACACTCACAGAAGGTGTAACGCGGCTAGCTGATATCAGTTTGCATGATCCAGTCAGTATTT CTGTCCTGGACAAGAGCCATGACCAGTTGAACCCAAAGGACAAAGCGGTCCAGGAGGTCTGTCCTCCACC AGCTGGCGACAAGCTGGACAGCTTTGCAATACCAGAGAGTCTCAAGCAGCATGTGACTGTGGTTCCCAGC AAACTGAGGCTTGTCTGCCTAGCGGCCTTCATCCTTCAGAAATGCAAGTTTGAGGAAGACCAGAAGATGG TTGTCTTTTTCTCAAGTTGCGAGCTGGTGGAGTTCCACTACAGCCTCTTCCTACAGACCCTGCTGAGCAG CTCAGGGGCGCCGGCATCAGGGCAGTTGCCATCTGCCTCCATGCGATTAAAATTCCTACGGCTGCATGGC GGCATGGAGCAGGAGGAAAGAACAGCAGTGTTTCAGGAATTTTCACATTCCAGAAGAGGCGTCCTTCTTT GCACG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA

Protein Sequence: >RC221612 representing NM_138620 Red=Cloning site Green=Tags(s)

MAPDLASQRHSESFPSVNSRPNVILPGREGRREGLPPGGGTRGSLVPTRPVPPSPAPLGTSPYSWSRSGP GRGGGAGSSRVPRGVPGPAVCAPGSLLHHASPTQTMAAADGSLFDNPRTFSRRPPAQASRQAKATKRKYQ ASSEAPPAKRRNETSFLPAKKTSVKETQRTFKGNAQKMFSPKKHSVSTSDRNQEERQCIKTSSLFKNNPD IPELHRPVVKQVQEKVFTSAAFHELGLHPHLISTINTVLKMSSMTSVQKQSIPVLLEGRDALVRSQTGSG KTLAYCIPVVQSLQAMESKIQRSDGPYALVLVPTRELALQSFDTVQKLLKPFTWIVPGVLMGGEKRKSEK ARLRKGINILISTPGRLVDHIKSTKNIHFSRLRWLVFDEADRILDLGFEKDITVILNAVNAECQKRQNVL LSATLTEGVTRLADISLHDPVSISVLDKSHDQLNPKDKAVQEVCPPPAGDKLDSFAIPESLKQHVTVVPS KLRLVCLAAFILQKCKFEEDQKMVVFFSSCELVEFHYSLFLQTLLSSSGAPASGQLPSASMRLKFLRLHG GMEQEERTAVFQEFSHSRRGVLLCT

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV Chromatograms: https://cdn.origene.com/chromatograms/mg2741_c01.zip

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 4 DDX31 (NM_138620) Human Tagged ORF Clone – RC221612

Restriction Sites: SgfI-RsrII Cloning Scheme:

Plasmid Map:

ACCN: NM_138620 ORF Size: 1755 bp

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 4 DDX31 (NM_138620) Human Tagged ORF Clone – RC221612

OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_138620.1, NP_619526.1 RefSeq Size: 2408 bp RefSeq ORF: 1443 bp Locus ID: 64794 UniProt ID: Q9H8H2 MW: 64 kDa Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2016]

Product images:

Western blot validation of overexpression lysate (Cat# [LY408455]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un- transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221612 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).