International Poultry Scientific Forum
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2019 INTERNATIONAL POULTRY SCIENTIFIC FORUM FEBRUARY 11 – 12 ABSTRACTS 2019 International Poultry Scientific Forum Georgia World Congress Center, Atlanta, Georgia February 11 - 12, 2019 Table of Contents ABSTRACTS Milton Y Dendy Keynote Address ....................................................................................................................................................... 1 Monday, February 11, 2019 Physiology, Endocrinology and Reproduction .................................................................................................................................... 2 Teaching, Pedagogy, Extension ........................................................................................................................................................... 5 Processing and Products ...................................................................................................................................................................... 6 Pathology ........................................................................................................................................................................................... 10 SCAD ................................................................................................................................................................................................. 13 Metabolism and Nutrition .................................................................................................................................................................. 18 Environment, Management and Animal Well-Being ......................................................................................................................... 34 Tuesday, February 12, 2019 Metabolism and Nutrition .................................................................................................................................................................. 41 SCAD ................................................................................................................................................................................................. 46 Metabolism and Nutrition .................................................................................................................................................................. 49 POSTER SESSIONS Teaching, Pedagogy, Extension ......................................................................................................................................................... 60 Physiology, Endocrinology and Reproduction .................................................................................................................................. 61 Processing and Products .................................................................................................................................................................... 63 Pathology ........................................................................................................................................................................................... 68 SCAD ................................................................................................................................................................................................. 71 Environment, Management and Animal Well-Being ......................................................................................................................... 75 Metabolism and Nutrition .................................................................................................................................................................. 83 AUTHOR INDEX ........................................................................................................................................ 109 ABSTRACTS 2019 International Poultry Scientific Forum Georgia World Congress Center, Atlanta, Georgia February 11 - 12, 2019 Milton Y Dendy Keynote Address B313 Courage Over Comfort - How Can You Cultivate Food Safety? Lone Jespersen Cultivate Impact of organizational culture on food safety is a much discussed topic in the global food industry and have directly been connected with consumer sickness and death. Companies who take this discussion serious have learned that it takes courage not only to understand and potentially assess their culture of food safety but also to ongoingly cultivate food safety to protect consumers, employees, and brands. In this session you will learn from both science and practical experiences how to breakdown your culture of food safety and what tactics you can apply tomorrow to create change and cultivate food safety in your company. 1 2 ABSTRACTS OF PAPERS *Author presenting paper GS Denotes Graduate Student Competition UG Denotes Undergraduate Presentation Monday, February 11, 2019 Physiology, Endocrinology and Reproduction M1 Broiler breeder hen zona pellucida B2 protein expression in bolster holistic health treatments. Breeders, as an upstream parameter, and the granulosa cell and perivitelline layer Josh Steed*1, Andrew Benson1, their health is an established co-factor in production of hatchable eggs, R Bramwell2, Adam Davis1 1The University of Georgia; 2University of but little is known about the influence of breeder gut health on down- Arkansas stream chick health. A direct line of transmission is predicted following the process of depositing fecal material onto egg shells and the association Prior to ovulation, the avian oocyte is surrounded by the theca, granulosa, of chicks to eggs shells after hatch. To investigate relationships of patho- and inner perivitelline layer (IPVL). The IPVL contains the zona pellucida gens in the breeder/hatch system, the enteric pathogen Avian Pathogenic (ZP) family of proteins which are required for sperm binding and fertiliza- E. coli (APEC) was isolated and measured for its possible transmission tion. Previously we reported that ZPB2 mRNA expression was greatest in through the hatchery environment and ultimately to the gastrointestinal the granulosa cells of pre-hierarchical follicles and declined as the follicle track (GIT) of the chick. Using RAPD fingerprint analysis of bacterial matured to ovulation. In addition, mRNA expression of ZPB2 was greater isolates, APEC from breeder GITs were matched to APEC found on the in the granulosa cells from the germinal disc (GD) region of hierarchical surface of egg shells hatchery environment, and within the 1 – 2 day-old follicles than in granulosa cells from nongerminal disc (NGD) regions. chick GITs. This relationship was found to be statistically significant us- The aim of the present research was to determine if the mRNA and pro- ing non-parametric methods for comparison. Other relationships were tein expression of ZPB2 correlated with one another. To determine ZPB2 identified, but to a lesser degree including hatchery air samples and dead protein expression in granulosa cells, the F1, F2, F3, and F4 hierarchical eggs, and dead eggs and chick GITs. Together, this work helps to highlight follicles were removed as were the small yellow (SYF) and large white the importance of pathogen transfer from breeder to chick, including the (LWF) pre-hierarchical follicles from twelve 63 week old broiler breeder hatchery as a vehicle for moving pathogens through the air. Importantly, hens. The SYF and LWF were further subdivided into two groups based the direct influence of gut health from breeder to chick demonstrate how on diameter. The tissue from 3 individual birds were pooled to generate gut health and pathogens share a common vehicle within poultry produc- 4 replicates samples of granulosa tissue for each follicle size. Protein was tion and should be targeted for interventions. isolated from GD and NGD sections of the IPVL of freshly laid eggs from twelve 44-week-old broiler breeder hens from 4 genetic lines of breeder Key Words: breeder, pathogen, transmission, gut-health hens. Individual GD and NGD sections were combined from 2 eggs from each line to create 6 replicate GD and NGD samples per genetic line. One M3 Bacillus subtilis 29784 prevents a pro-inflammatory response dimensional gel electrophoresis was performed using stain free technol- in an induced inflammation condition using the Caco-2 cells model ogy, and gels were transferred to PVDF membranes for Western blot Damien Preveraud*1, Lamya Rhayat2, Marc Maresca3, Erik Eckhardt2, analysis. A polyclonal antibody (rabbit anti-chicken) against ZPB2, and Estelle Devillard2 1Adisseo France SAS; 2Adisseo CERN; 3Aix Marseille HRP conjugated secondary antibody (goat anti-rabbit) were used for the University CNRS Western blot analyses. Relative ZPB2 expression was determined using Image Lab Software (BioRad). Granulosa cell ZPB2 protein expression The protection of the animals against exogenous challenges, including was greater in the smallest LWF relative to any of the hierarchical follicles infectious diseases and digestive disorders, is mainly provided by the in- (P < 0.05). Protein expression of ZPB2 was greater in the GD region than testine. Maintaining intestinal barrier integrity and functions is therefore NGD region of the perivitelline layer. Overall, ZPB2 protein expression critical to avoid deterioration of animal health status. Among the differ- reflected mRNA expression in broiler breeder hens. These findings sug- ent ways to achieve this, probiotic supplementation could turn out to be a gest an undetermined role for ZPB2 in early follicular development and good solution.