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OriGene Technologies, Inc. OriGene Technologies GmbH

9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

AM01322PU-N Monoclonal to CD79a (202-216) - Purified

Alternate names: B-Cell marker, B-cell antigen receptor complex-associated alpha-chain, IGA, MB-1 membrane glycoprotein, MB1, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein Quantity: 0.2 mg Concentration: 1.0 mg/ml Background: The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non- covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin. Uniprot ID: P11912 NCBI: NP_001774.1 GeneID: 973 Host / Isotype: Mouse / IgG1 Recommended Isotype SM10P (for use in human samples), SM20P (for use in rat samples), AM03095PU-N Controls: Clone: HM57 Immunogen: Synthetic peptide corresponding to 202-216 sequence of Human mb-1. Genename: CD79a Remarks: Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line. Format: State: Liquid purified IgG fraction from Tissue Culture Supernatant Purification: Affinity Chromatography on Protein G Buffer System: PBS Preservatives: 0.09% Sodium Azide Applications: Western blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/100-1/500. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human tonsil. Flow Cytometry: Use 10 µl of 1/10-1/50 diluted CD79a antibody to label 1x106 cells in 100 µl (Membrane permeabilisation is required). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-.com or on request. MS/20170405 1 / 3 AM01322PU-N: Monoclonal Antibody to CD79a (202-216) - Purified

Specificity: This antibody recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. : Human, Canine (), Monkey, , Horse, Bovine, , Rat, Mouse, , Chicken, Fallow , Red deer, American . Other species not tested. Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one from despatch. General Readings: 1. Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, et al. The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147(11):2474-82. PubMed PMID: 1747162. 2. Jones M, Cordell JL, Beyers AD, Tse AG, Mason DY. Detection of T and B cells in many species using cross-reactive anti-peptide antibodies. J Immunol. 1993 Jun 15;150(12):5429-35. PubMed PMID: 8515069. 3. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update. Vet Res. 39: 54. 4. Christgau M, Caffesse RG, Newland JR, Schmalz G, D'Souza RN. Characterization of immunocompetent cells in the diseased canine periodontium. J Histochem Cytochem. 1998 Dec;46(12):1443-54. PubMed PMID: 9815286. 5. Spaas, J.H. et al. (2012) Culture and characterisation of equine peripheral blood mesenchymal stromal cells. Vet J. Jun 18. [Epub ahead of print] 6. Del Cacho, E. et al. (2009) Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 2009 May 15;129(1-2):66-75. Epub 2008 Dec 11. 7. Nelson DD, Davis WC, Brown WC, Li H, O'Toole D, JL. CD8(+)/perforin(+)/WC1(-) gammadelta T cells, not CD8(+) alphabeta T cells, infiltrate vasculitis lesions of (Bison bison) with experimental sheep- associated malignant catarrhal fever. Vet Immunol Immunopathol. 2010 Aug 15;136(3-4):284-91. doi: 10.1016/j.vetimm.2010.03.023. Epub 2010 Apr 1. PubMed PMID: 20413164. 8. Spaas JH, De Schauwer C, Cornillie P, Meyer E, Van Soom A, Van de Walle GR. Culture and characterisation of equine peripheral blood mesenchymal stromal cells. Vet J. 2013 Jan;195(1):107-13. doi: 10.1016/j.tvjl.2012.05.006. Epub 2012 Jun 18. PubMed PMID: 22717781.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20170405 2 / 3 AM01322PU-N: Monoclonal Antibody to CD79a (202-216) - Purified

Pictures: Staining of paraffin embedded human tonsil with mouse anti human CD79a antibody .-No. AM01322PU.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20170405 3 / 3

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