West African Venomous Snakes Identification
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An in Vivo Examination of the Differences Between Rapid
www.nature.com/scientificreports OPEN An in vivo examination of the diferences between rapid cardiovascular collapse and prolonged hypotension induced by snake venom Rahini Kakumanu1, Barbara K. Kemp-Harper1, Anjana Silva 1,2, Sanjaya Kuruppu3, Geofrey K. Isbister 1,4 & Wayne C. Hodgson1* We investigated the cardiovascular efects of venoms from seven medically important species of snakes: Australian Eastern Brown snake (Pseudonaja textilis), Sri Lankan Russell’s viper (Daboia russelii), Javanese Russell’s viper (D. siamensis), Gaboon viper (Bitis gabonica), Uracoan rattlesnake (Crotalus vegrandis), Carpet viper (Echis ocellatus) and Puf adder (Bitis arietans), and identifed two distinct patterns of efects: i.e. rapid cardiovascular collapse and prolonged hypotension. P. textilis (5 µg/kg, i.v.) and E. ocellatus (50 µg/kg, i.v.) venoms induced rapid (i.e. within 2 min) cardiovascular collapse in anaesthetised rats. P. textilis (20 mg/kg, i.m.) caused collapse within 10 min. D. russelii (100 µg/kg, i.v.) and D. siamensis (100 µg/kg, i.v.) venoms caused ‘prolonged hypotension’, characterised by a persistent decrease in blood pressure with recovery. D. russelii venom (50 mg/kg and 100 mg/kg, i.m.) also caused prolonged hypotension. A priming dose of P. textilis venom (2 µg/kg, i.v.) prevented collapse by E. ocellatus venom (50 µg/kg, i.v.), but had no signifcant efect on subsequent addition of D. russelii venom (1 mg/kg, i.v). Two priming doses (1 µg/kg, i.v.) of E. ocellatus venom prevented collapse by E. ocellatus venom (50 µg/kg, i.v.). B. gabonica, C. vegrandis and B. -
Year of the Snake News No
Year of the Snake News No. 3 March 2013 www.yearofthesnake.org The Value of Snakes - By: Polly Conrad, The Orianne Society Snake Venom Can Save Your Life or disorders, you should support • A southern Copperhead snake conservation! In this article, I (Agkistrodon contortrix) venom present a brief overview of some of protein, called contortrostatin, pre- the medicinal values of snakes. Who vents cancer cells from attaching to would have thought snake venom other cells and also prevents them could be life-saving? from producing the signals neces- It all starts with living, breathing, sary to prompt new blood vessels venomous snakes, which are milked to sprout and support the spread by professionals for their venom. of cancer. Contortrostatin curbed The venom samples are then sent to the spread of cancer by 90% in laboratories for various analyses and mice implanted with breast cancer testing. Venom is a blend of mol- tumors! Copperhead, Agkistrodon contortrix. ecules, including enzymes, peptides, Photo © John White. • A novel King Cobra (Ophiophagus and proteins. Many studies have hannah) venom protein, haditoxin, Even if you don’t like snakes, identified several benefits provided may be useful as a ‘molecular chances are that you or someone you by snake venom proteins. I’ve listed probe’ which will help researchers know can benefit from the research some below. study neurotransmitter receptors and applications surrounding snake • The protein, ancrod, from the and their roles in neurodegenera- venom proteins. These proteins are Malayan Pit Viper (Callaselasma tive conditions such as Alzheimer’s being used to study, treat and cure rhodostoma) is being studied to and Parkinson’s diseases, as well as heart disease, high blood pressure, treat patients suffering from deep schizophrenia, anxiety, and depres- stroke, Alzheimer’s disease and vein blood clots or stroke, and to sive disorders and even nicotine cancer. -
THE PUFF ADDER (BITIS ARIETANS) J.L. CLOUDSLEY-THOMPSON Department of Biology (Medawar Building), University College, University
British Herpetological Society Bulletin, No. 26, 1988. THE PUFF ADDER (BITIS ARIETANS) J.L. CLOUDSLEY-THOMPSON Department of Biology (Medawar Building), University College, University of London, Gower Street, London WCIE 6BT The Puff Adder (Bitis arietans) is one of the largest of the African vipers and probably the species most frequently seen by travellers in that continent. It receives its English name from the habit of inflating its body and hissing loudly when disturbed. The sound is produced both when the breath is inhaled as well as during exhalation. This behaviour is characteristic of all true vipers, but is particularly evident in the case of the Puff Adder. Unlike the Gaboon Viper (Bitis gabonica), which is a forest snake, the Puff Adder inhabits subdeserts and savannas, and is also to be found in mountainous regions. Except in rain forests, Puff Adders are widespread southward to the Cape from Morocco in the west and the Sudan in the east. They occur as near to Khartoum as Jebel Aulia. This is their northernmost limit in Sudan, but they range also into western and southern Arabia. Puff Adders may exceed 1.4m in length, and have a girth of 25cm. Although they do not attain the weight of a full-grown Gaboon Viper they are, nevertheless, formidable snakes. There may be considerable variation in the coloration of Puff Adders. In some specimens, the chevrons are sooty black and the crescents cream coloured while, in others, the chevrons are dark brown or grey and the crescents dull buff. The blotched pattern of dark chevrons separated by yellow crescents (Plate 1) is cryptic. -
A Formal Five-Way Division of the Gaboon Viper Species Complex: Bitis
Australasian Journal of Herpetology Australasian Journal of Herpetology 16:25-31. ISSN 1836-5698 (Print)25 Published 10 July 2013. ISSN 1836-5779 (Online) A formal five-way division of the Gaboon Viper Species Complex: Bitis (Macrocerastes) gabonica (Duméril, Bibron and Duméril, 1854) and a two-way division of the Nose-horned Viper species complex Bitis (Macrocerastes) nasicornis (Shaw, 1802) (Serpentes:Viperidae:Bitisini). RAYMOND T. HOSER 488 Park Road, Park Orchards, Victoria, 3114, Australia. Phone: +61 3 9812 3322 Fax: 9812 3355 E-mail: [email protected] Received 21 April 2013, Accepted 4 June 2013, Published 10 July 2013. ABSTRACT The Gaboon Viper Bitis gabonica (Duméril, Bibron and Duméril, 1854) as a species complex has had a fairly stable taxonomic history since being described at the species level, although the species as generally recognized was transferred to the genus Bitis Gray, 1842 shortly after the original description. Likewise for the Nose-horned Viper species complex Bitis (Macrocerastes) nasicornis (Shaw, 1802). The species known as the Rhinoceros Viper Bitis rhinoceros (Schlegel, 1855) was synonymised with Bitis gabonica by virtually all herpetologists beyond 1855 until 1999 (see McDiarmid et al. 1999), when Lenk et al. (1999) provided a molecular basis to recognize the western population, (then known as Bitis gabonica rhinoceros) identified in 1999 on the basis of allopatric distribution as opposed to any consistent morphological divergence. Chippaux (2006), showed that consistent differences in the markings on the side of the head could be used to identify and separate Bitis rhinoceros from the nominate species. Meanwhile the Gaboon Viper as popularly recognized since 1999 comprises the main population centred on the wetter parts of west-central Africa (the type locality Gabon) including several countries and then three quite distant and unconnected outlier populations. -
For All Snakebites Visit a Health Facility Immediately!
Contact Royjan Taylor for emergencies: +254718290324 (also available on whatsapp) FOR ALL SNAKEBITES VISIT A HEALTH FACILITY Royjan Taylor Anton Childs David Warrell Anton Childs Wolfgang Wuster Black Mamba Black Necked Blanding’s Tree Boomslang East African Dendroaspis polylepis Spitting Cobra Snake female / male Dispholidus typus Garter Snake IMMEDIATELY! Naja nigricollis Toxicodryas blandingii Elapsoidia loveridgei Wolfgang Wuster Anton Childs Maik Dobiey Patrick Malonza Maik Dobiey David Warrell Maik Dobiey David Warrell Eastern Green Egyptian Cobra Forest Cobra Forest Night Adder Gaboon Viper Gold’s Tree Cobra Green Bush Viper Jameson’s Mamba Mamba Naja haje Naja melanoleuca Causus lichtensteinii Bitis gabonica Pseudohaje goldii Atheris squamiger Dendroaspis jamesoni kaimosi Dendroaspis angusticeps David Warrell Maik Dobiey Wolfgang Wuster Royjan Taylor Danie Theron Anton Childs Royjan Taylor Danie Theron Kenya Horned Viper Kenya Montane Large Brown Mount Kenya North East African Puff Adder Red Spitting Cobra Rhinoceros Viper Bitis wothingtoni Viper Spitting Cobra Bush Viper Carpet Viper Bitis arietans Naja pallida Bitis nasicornis Montatheris hindii Holotype / Naja ashei Atheris desaixi Echis pyramidum Common Venomous Snakes of Kenya Anton Childs Royjan Taylor Anton Childs Anton Childs Royjan Taylor Royjan Taylor Royjan Taylor Rhombic Night Rough-Scaled Bush Savannah Vine Small-Scaled Snouted Night Velvet Green Night Yellow Bellied Sea Adder Viper Snake or Twig Snake Mole Viper Adder Adder Snake Causus rhombeatus Atheris hispida Thelotornis mossambicanus Atractaspis microlepidota Causus defilippi Causus resimus Pelamis platurus. -
Abstracts Content
Abstracts Content Keynotes 1 Oral Presentations 11 Behavioral Biology 12 Developmental Biology 32 Ecology 46 Evolutionary Biology 60 Morphology 86 Neurobiology 100 Physiology 114 Zoological Systematics 128 Keynotes Poster Presentations 142 Behavioral Biology 143 Developmental Biology 160 Ecology 166 Evolutionary Biology 173 Morphology 193 Neurobiology 211 Physiology 248 Zoological Systematics 258 Satellite Symposia 266 Satellite Symposium I 267 Arthropod Neuro Network (ANN) Satellite Symposium II 278 Epigenetics in Animal Physiology Satellite Symposium III 281 Insect Biotechnology Satellite Symposium IV 299 Animal Phylogeny - Past, Present and Future Author Index 311 1 Keynotes KN-01 KN-02 Oral Presentations Insect Biotechnology C. elegans in an Evolutionary and Ecological Context: Vulva Development and Natural Pathogens 1,2 Poster A. Vilcinskas Presentations 1Institute of Phytopathology and Applied Zoology, Justus-Liebig- M.- A. Félix1 University of Gießen, Applied Entomology, Gießen, Germany 1Institut de Biologie de l’Ecole Normale Supérieure, CNRS-ENS-Inserm, Satellite Symposia 2Fraunhofer Institute of Molecular Biology and Applied Ecology, Paris, France Department Bioresources, Gießen, Germany Biological processes are generally studied in the laboratory under one Insect Biotechnology, alternatively called yellow biotechnology, has environmental condition and in one reference genetic background. We been defined as the use of biotechnology-based approaches to exploit try to widen this horizon to answer questions on the relationship between insects or insect-derived molecules, cells, organs or microorganisms genetic and phenotypic evolution, by placing a paradigmatic model for development of products or services for mediacl, agricultural or system in developmental biology, C. elegans vulval cell fate patterning, in industrial applications. This ermerging field is developed within the its evolutionary context. -
Cottonmouth, Most People Think That the Caduceus Caduceus
id you know that Alabama is the fifth most biodiverse state Humans have used toxins for in the country – with more species of plants and animals medical purposes throughout history; Dthan almost any other state! Reptiles and amphibians, or Ancient Greece, traditional Chinese “herps”, are large contributors to our title. We have over 50 kinds of medicine, and on to modern-day. snakes in our state, only 6 of which are venomous: the Cottonmouth, Most people think that the Caduceus Caduceus Copperhead, Eastern Coral Snake, Timber Rattlesnake, Eastern is the medical symbol, but it’s actually Rod of Asclepius Diamondback Rattlesnake, and Pigmy Rattlesnake. When most the Rod of Asclepius that is the true PHOTOS BY WIKIMEDIA people see or hear about snakes, they immediately think, “The only symbol of healthcare organizations and good snake is a dead snake!” That couldn’t be farther from the truth.. medical practice. The Greek God Asclepius is a deity associated with All snakes play a beneficial role in our ecosystem. healing and medicine. The serpent in the symbol is said to symbolize Many people refer to venomous snakes as “poisonous”. While rejuvenation through the shedding of skin or the dual nature of a both venom and poison are toxins, they differ dramatically. physician’s work in dealing with life and death. The ambiguity of the Venomous animals, snakes and others, inject their venom using serpent as a symbol is representative of the ambiguity of drug use, fangs, stingers, or other specialized apparatuses. The venom is which can heal or harm. More testament to this contradiction is the produced in a gland inside of the fangs and stingers. -
African Adders: Partial Characterization of Snake Venoms from Three Bitis Species of Medical Importance and Their Neutralization by Experimental Equine Antivenoms
RESEARCH ARTICLE African Adders: Partial Characterization of Snake Venoms from Three Bitis Species of Medical Importance and Their Neutralization by Experimental Equine Antivenoms Danielle Paixão-Cavalcante, Alexandre K. Kuniyoshi, Fernanda C. V. Portaro, Wilmar Dias da Silva, Denise V. Tambourgi* Immunochemistry Laboratory, Butantan Institute, São Paulo, Brazil * [email protected] Abstract Background An alarming number of fatal accidents involving snakes are annually reported in Africa and most of the victims suffer from permanent local tissue damage and chronic disabilities. En- OPEN ACCESS venomation by snakes belonging to the genus Bitis, Viperidae family, are common in Sub- Citation: Paixão-Cavalcante D, Kuniyoshi AK, Saharan Africa. The accidents are severe and the victims often have a poor prognosis due Portaro FCV, da Silva WD, Tambourgi DV (2015) to the lack of effective specific therapies. In this study we have biochemically characterized African Adders: Partial Characterization of Snake venoms from three different species of Bitis, i.e., Bitis arietans, Bitis gabonica rhinoceros Venoms from Three Bitis Species of Medical and Bitis nasicornis, involved in the majority of the human accidents in Africa, and analyzed Importance and Their Neutralization by Experimental Equine Antivenoms. PLoS Negl Trop Dis 9(2): the in vitro neutralizing ability of two experimental antivenoms. e0003419. doi:10.1371/journal.pntd.0003419 Editor: Jean-Philippe Chippaux, Institut de Methodology/Principal Findings Recherche pour le Développement, BENIN The data indicate that all venoms presented phospholipase, hyaluronidase and fibrinogen- Received: March 6, 2014 olytic activities and cleaved efficiently the FRET substrate Abz-RPPGFSPFRQ-EDDnp and – Accepted: November 15, 2014 angiotensin I, generating angiotensin 1 7. -
Carr, J. 2015. Species Monitoring Recommendations for the Transboundary Area of Greater Gola Peace Park
Communication Strategy (PARCC Activity 4.2) Ver. 1. Protected Areas Resilient to Climate Change, PARCC West Africa 2015 Species monitoring recommendations for the G reater Gola Peace Park (Liberia and Sierra Leone) ENGLISH Jamie Carr IUCN Global Species Programme 2015 Species monitoring recommendations: Greater Gola Peace Park. The United Nations Environment Programme World Conservation Monitoring Centre (UNEP-WCMC) is the specialist biodiversity assessment centre of the United Nations Environment Programme (UNEP), the world’s foremost intergovernmental environmental organisation. The Centre has been in operation for over 30 years, combining scientific research with practical policy advice. Species monitoring recommendations for the greater Gola Peace Park (Liberia and Sierra Leone), prepared by Jamie Carr., with funding from Global Environment Facility (GEF) via UNEP. Copyright: 2015. United Nations Environment Programme. Reproduction This publication may be reproduced for educational or non-profit purposes without special permission, provided acknowledgement to the source is made. Reuse of any figures is subject to permission from the original rights holders. No use of this publication may be made for resale or any other commercial purpose without permission in writing from UNEP. Applications for permission, with a statement of purpose and extent of reproduction, should be sent to the Director, DCPI, UNEP, P.O. Box 30552, Nairobi, Kenya. Disclaimer: The contents of this report do not necessarily reflect the views or policies of UNEP, contributory organisations or editors. The designations employed and the presentations of material in this report do not imply the expression of any opinion whatsoever on the part of UNEP or contributory organisations, editors or publishers concerning the legal status of any country, territory, city area or its authorities, or concerning the delimitation of its frontiers or boundaries or the designation of its name, frontiers or boundaries. -
Comparative Studies of 'Bile Salts' 13
352 M. L. DEDMAN, T. H. FARMER AND C. J. 0. R. MORRIS 1961 Farmer, T. H. (1959). Biochem. J. 73, 321. Hill, R. L. & Smith, E. L. (1957). J. biol. (Chem. 228, Farmer, T. H. & Morris, C. J. 0. R. (1956). Nature, Lond., 577. 178, 1465. Morris, C. J. 0. R. (1951). Analy8t, 76, 470. Gross, D. (1956). Nature, Lond., 178, 29. Sayers, M. A., Sayers, G. & Woodbury, L. A. (1948). Hayes, E. E. & White, W. F. (1954). Recent Progr. Hormone Endocrinology, 42, 378. Res. 10, 265. Shepherd, R. G., Wilson, S. D., Howard, K. S., Bell, P. H., Heilmann, J., Barollier, J. & Watzke, E. (1957). Hoppe- Davies, D. S., Davis, S. B., Eigner, E. A. & Shakespeare, Seyl. Z. 30, 219. N. E. (1956). J. Amer. chem. Soc. 78, 5067. Biochem. J. (1961) 78, 352 Comparative Studies of 'Bile Salts' 13. BILE ACIDS OF THE LEOPARD SEAL, HYDRURGA LEPTONYX, AND OF TWO SNAKES OF THE GENUS BITIS* BY G. A. D. HASLEWOOD Guy'8 Ho8pital Medical School, London, S.E. 1 (Received 5 July 1960) In the preliminary survey with which this work the corresponding esters of leopard-seal and began the bile of ten species of snakes was ex- Californian-sealion bile acids. Chromatography on amined. The Boidae (three species) gave pytho- alumina of the ethyl esters of Gaboon-viper bile cholic lactone (3cc: 12a: 16ac-trihydroxycholanic acid acids easily separated the fraction responsible for lactone), six other species yielded cholic acid spot P from a 'spot Q-ethyl cholate' fraction, (3x:7a:12x-trihydroxycholanic acid), but from the described later. -
Venom Yields from Australian and Some Other Species of Snakes
Ecotoxicology (2006) 15:531–538 DOI 10.1007/s10646-006-0089-x Venom yields from Australian and some other species of snakes Peter J. Mirtschin Æ Nathan Dunstan Æ Ben Hough Æ Ewan Hamilton Æ Sharna Klein Æ Jonathan Lucas Æ David Millar Æ Frank Madaras Æ Timothy Nias Accepted: 19 May 2006 / Published online: 26 August 2006 Ó Springer Science+Business Media, LLC 2006 Abstract The wet and dry venom yields for most solids or dry yield is normally just called the yield as Australian native dangerous snakes and a number of opposed to the wet yield which is the total weight of non-Australian species are presented. Snakes from the the venom (water plus all the solids). The percentage Pseudonaja genus yielded higher than previously pub- solids are the fraction of the solid weight divided by the lished amounts and suggest reconsideration be given to total weight (solids and liquid) of the venom expressed increasing the volume of antivenom in each vial. as a percentage [(solid weight/total weight) · 100 = % Higher percentage solids were obtained from venoms solids]. from the 4 cobra species (Naja) and Pseudechis genus There have been many ways in which snakes have included in this series. been artificially milked for their venom and indeed many ways of drying venom. Some early milking Keywords Venom Æ Yield Æ Snake techniques included forcing the snake to bite on a watch glass whereupon the venom spread out over the glass. Another method was to force the snake to drape Introduction its fangs over the side of a beaker and allow the venom to run down the inside of the beaker (a method more The amount of venom produced by a snake when it suited to longer-fanged vipers). -
Effect of Gaboon Viper (Bitis Gabonica) Venom on Blood Coagulation, Platelets, and the Fibrinolytic Enzyme System
J Clin Pathol: first published as 10.1136/jcp.22.3.312 on 1 May 1969. Downloaded from J. clin. Path. (1969), 22, 312-316 Effect of gaboon viper (Bitis gabonica) venom on blood coagulation, platelets, and the fibrinolytic enzyme system C. D. FORBES, A. G. G. TURPIE, J. C. FERGUSON, G. P. McNICOL, AND A. S. DOUGLAS From Makerere University College Medical School Extension, Nairobi, and the University Department of Medicine, Royal Infirmary, Glasgow SYNOPSIS The action of the venom of the gaboon viper (Bitis gabonica) on blood coagulation, platelets, and the fibrinolytic enzyme system was studied. The results confirm that the venom of Bitis gabonica has a marked anticoagulant action in vitro. The venom appears to impair clot formation by a direct proteolytic action on fibrinogen, releasing soluble breakdown products. A bite from a gaboon viper results in severe PLASMA RECALCIFICATION TIME Various concentrations destruction of local tissue and a systemic haemor- of venom were added to this test system (as described by rhagic state with bleeding from mucosal and serosal Biggs and Macfarlane, 1962) in place of the saline control. surfaces The mechanisms by which (Grasset, 1946). THROMBIN GENERATION TEST This was performed as the venom produces these effects have not been described by Biggs and Macfarlane (1962) using whole adequately studied but the anticoagulant action has blood. Venom was added to one tube and saline as a been ascribed to 'fibrinolytic' properties. However, control to another. http://jcp.bmj.com/ Christensen (1955) failed to find a correlation between the ability of the venom to digest carmine- THROMBIN-FIBRINOGEN DILUTION CURVE This was cal- stained fibrin and its effect on the clotting mechan- culated as described by Biggs and Macfarlane (1962).