Recombinant Human GPA33/Glycoprotein-A33 Protein

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Catalog No.: RP00163 Recombinant

Sequence Information Background

Species Gene ID Swiss Prot Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I Human 10223 Q99795 membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) Tags domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading C-6×His frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a Synonyms 213-amino acid extracellular region, a single transmembrane domain, and a 62- A33 amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation.

Product Information Basic Information

Source Purification Description HEK293 cells > 95% by SDS- Recombinant Human GPA33/Glycoprotein-A33 Protein is produced by HEK293 cells PAGE. expression system. The target protein is expressed with sequence (Ile22-Val235) of human GPA33/Glycoprotein-A33 (Accession #NP_005805.1) fused with a 6×His tag Endotoxin at the C-terminus. < 0.1 EU/μg of the protein by LAL method. Bio-Activity

Formulation Storage Lyophilized from a 0.22 μm filtered Store the lyophilized protein at -20°C to -80 °C for long term. solution of PBS, pH 7.4.Contact us for After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C customized product form or for up to 1 week. formulation. Avoid repeated freeze/thaw cycles.

Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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www.abclonal.com Validation Data

Recombinant Human GPA33/Glycoprotein- A33 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-40 kDa.