An Investigation of Disturbance, Sampling Methods, the River Continuum Con�ept, and Hydroseres in Hardwood Creek, a Ditched Stream in Minnesota, USA

Volume II

A THESIS SUBMITTED TO THE FACULITY OF THE GRADUATE SCHOOL OF THE UNIVERSITY OF MINNESOTA BY

Adam Wayne Sealock

IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE

Advisor Dr. Leonard C. Ferrington Jr.

September 2007 Table of Contents

Acknowledgements

Abstract iii

List of Tables viii

List of Figures xiii

Chapter 1: Introduction, Literature Review, and· Organization of Thesis

Introduction 1

Literature Review: An Overview of Disturbance Ecology With an Emphasis on Lotic Ecosystems 2

Organization of Thesis 15

Literature Cited 18

Chapter 2: Evaluation of Two Sampling Methods in Hardwood Creek, a Ditched Stream in Minnesota, USA

Abstract 25

Introduction 26

Methods 27

Results 39

Discussion 40

Literature Cited 45

Chapter 3: An Investigation of the Intermediate Disturbance Hypothesis in Hardwood Creek, a Ditched Stream in Minnesota, USA

Abstract 54

Introduction 55

Methods 57

Results 63

Discussion 65 Literature Cited 73

Chapter 4: A Test of the River Continuum Concept in Hardwood Creek, a Ditched Stream in Minnesota, USA

Abstract 92

Introduction 93

Methods 97

Results 98

Discussion 98

. Literature Cited 102

Chapter 5: Hydroseres and Management Categories as Classifications for Understanding Community Composition of Macroinvertebrates in Hardwood Creek, a Ditched Stream in Minnesota, USA

Abstract 110

Introduction 111

Methods 112

Results 113

Discussion 115

Literature Cited 124

Chapter 6: A Literature Review of (Diptera) Pupal Exuviae Use in Ecological Assessment Studies of Lotic Ecosystems

Introduction 135

Discussion 135

Literature Cited 141

Chapter 7: Sampling Efficiency of Chironomidae (Diptera) Across Disturbance Gradients

Abstract 145

Introduction 145

Materials and Methods 147

11 Results 148

Discussion 149

Conclusions 151

References 153

Appendix A: Keys and Descriptions of the Chironomidae Pupal Exuviae Collected in Hardwood Creek, Minnesota, USA

Introduction 158

References 222

Appendix B: Supplemental Tables and Figures

Introduction 225

Appendix C: A Comprehensive List of Macroinvertebrates in Hardwood Creek

Introduction 286

lll List of Tables

Chapter 2

Table 1. The number of ON samples collected from each habitat stratification unit at each site in Hardwood Creek. 49

Table 2. Sampling dates in 2004 for collection of SFPE samples at each site. 49

Table 3. The number of taxa collected with ON or SFPE methods within each order (unless labeled other wise). 50

Table 4. The totals of non-chironomid taxa collected exclusively with ON and SFPE samples, and those collected with both methods. 51

Chapter 3

Table 1. The number of taxa collected for each order, unless otherwise labeled, in ON and SFPE samples. 79

Table 2. The non-Oiptera fa milies and the number of taxa collected for each in ON and SFPE samples. 80

Table 3. The families of Oiptera, Chironomidae subfamilies and tribes, and the number of taxa collected in both ON and SFPE samples. 81

Table 4. taxa by lentic, lotic, or both lentic/lotic habitat preference for sites 1.5U and 1.4 based on Merritt and Cummings (1996). 81

Table 5. The totals for non-chironomid taxa collected exclusively in ON and SFPE samples, and those collect with both methods. 82

Chapter 4

Table 1. The 8 RCC hypotheses tested in Hardwood Creek. 103

Table 2. The ranks of site similarities (lower section) based upon the SMC index for ON samples and the index values (upper section) used to assign ranks for each site pair for hypothesis 1. 1 03

Table 3. The ranks of site similarities (lower section) based upon the SMC index for chironomid larvae and the index values (upper section) used to assign ranks for each site pair for hypothesis 2. 104

Table 4. The ranks of site similarities (lower section) based upon the SMC index for SFPE and the index values (upper section) used to assign ranks for each site pair for hypothesis 3. 104

IV Table 5. The ranks of site similarities (lower section) based.upon the JAC index for DN samples and the index values (upper section) used to assign ranks for each site pair for hypothesis 4. 105

Table 6. The ranks of site similarities (lower section) based upon the JAC index for chironomid larvae and the index values (upper section) used to assign ranks for each site pair for hypothesis 5. 1 05

Table 7. The ranks of site similarities (lower section) based upon the JAC index for SFPE and the index values (upper section) used to assign ranks for each site pair for hypothesis 6. 106

Table 8. The ranks of site similarities (lower section) based upon the WPS index for DN samples and the index values (upper section) used to assign ranks for each site pair for hypothesis 7. 106

Table 9. The ranks of site similarities (lower section) based upon the WPS index for chironomid larvae and the index values (upper section) used to assign ranks for each site pair for hypothesis 8. 107

Table 10. A hypothetical matrix of site-by-site comparisons in which ranks of similarities conform to the RCC. 107

Table 11. P-values from exact Wilcoxon rank sum tests of the RCC in Hardwood Creek for SMC, JAC, and WPS similarity indices, calculated from DN, chironomid larvae (L) and SFPE data sets. 108

Chapter 5

Table 1. The test for significance between upstream and downstream sites. 128

Chapter 7

Table 1. Present and sampled habitats at each site. 155

Table 2. The metrics, site comparisons, collection dates, means, and p-values used to evaluate 8 hypotheses of chironomid larval and pupal sample efficiency. 155

Table 3. The number of specimens collected ESFPE and EON. 156

Appendix B

Chapter 2

Table 1. The mean, minimum (min.), and maximum (max.) similarity index values calculated for SMC, JAC, and WPS indices for all possible site combinations. 225

Table 2. The correlation coefficients based on similarity index values calculated for all site comparisons for the ON and larvae (DN/L), DN/SFPE, and larvae and SFPE (L/SFPE) comparisons. 225

v Chapter 5

Table 3. The recently, intermediately, and not disturbed hydroseres for ON, chironomid larvae, and SFPE methods. 249

Table 4. The recent and intermediate disturbed, recent and not disturbed, and intermediate and not disturbed for ON, chironomid larvae, and SFPE methods. 249

Table 5. Upstream, downstream, across upstream and downstream sites, and their average values for ON, chironomid larvae, and SFPE methods. 250

Appendix C

Table 1. Cumulative taxa list for ON samples at site 1.5U. 286

Table 2. Cumulative taxa list for ON samples at site 1.4. 297

Table 3. Cumulative taxa list for ON samples at site New. 307

Table 4. Cumulative taxa list for ON samples at site 1.3. 318

Table 5. Cumulative taxa list for ON samples at site 1.50. 328

Table 6. Cumulative taxa list for ON samples at site 1. 339

Table 7. Cumulative taxa list for ON samples at site 1.1. 349

Table 8. Cumulative taxa list for ON samples at site 1.2. 360

Table 9. Cumulative taxa list for ON samples at site 2. 370

Table 10. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1.5U. 381

Table 11. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1.4. 390

Table 12. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1.3. 399

Table 13. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1.50. 408

Table 14. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1. 416

Table 15. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1.1. 425

VI Table 16. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1.2. 433

Table 17. Cumulative taxa list for macroinvertebrates from SFPE samples at site 2. 442

Table 18. Cumulative taxa list for SFPE from site 1.5U identified under a dissection microscope. 451

Table 19. Cumulative taxa list for SFPE from site 1.4 identified under a dissection microscope. 455

Table 20. Cumulative taxa list for SFPE from site 1.3 identified under a dissection microscope. 459

Table 21. Cumulative taxa list for SFPE from site 1.50 identified under a dissection microscope. 463

Table 22. Cumulative taxa list for SFPE from site 1 identified under a dissection microscope. 467

Table 23. Cumulative taxa list for SFPE from site 1.1 identified under a dissection microscope. 471

Table 24. Cumulative taxa list for SFPE from site 1.2 identified under a dissection microscope. 475

Table 25. Cumulative taxa list for SFPE from site 2 identified under a dissection microscope. 4 79

Table 26. Cumulative taxa list for SFPE from site 1.5U subsampled and identified under a compound microscope. 484

Table 27. Cumulative taxa list for SFPE from site 1.4 subsampled and identified under a compound microscope. 488

Table 28. Cumulative taxa list for SFPE from site 1.3 subsampled and identified under a compound microscope. 492

Table 29. Cumulative taxa list for SFPE from site 1.50 subsampled and identified under a compound microscope. 496

Table 30. Cumulative taxa list for SFPE from site 1 subsampled and identified under a compound microscope. 500

Table 31 . Cumulative taxa list for SFPE from site 1.1 subsampled and identified under a compound microscope. 504

Table 32. Cumulative taxa list for SFPE from site 1.2 subsampled and identified under a compound microscope. 508

Vll List of Figures

Chapter 1

Figure 1. A map of Hardwood Creek and the 9 sample sites. 24

Chapter 2

Figure 1. A map of Hardwood Creek and the 9 sample sites. 52

Figure 2. The totals of non-chironomid and chironomid taxa collected exclusively in ON, exclusively in SFPE, and with both methods. 52

Figure 3. Similarity means for each index across all possible site combinations for taxa from ON, chironomid larvae, and SFPE data sets. 53

Figure 4. The correlation coefficients of similarity indices for the ON and larvae (ON/L), ON/SFPE, and larvae and SFPE (L/SFPE) comparisons for SMC, JAC, and WPS indices where applicable. 53

Chapter 3

Figure 1. A map of Hardwood Creek and the 9 sample sites. 83

Figure 2. Taxa richness for all taxa from ON and SFPE samples for hypothesis one. 83

Figure 3. The estimate, not the mean, of Brillouin's index in nats base upon all ON samples at each site. 84

Figure 4. Neotaenioglossa richness for ON and SFPE samples for hypothesis three. 84

Figure 5. Rhynchobdellida richness for ON and SFPE samples for hypothesis three. 85

Figure 6. Oytiscidae richness in ON and SFPE samples for hypothesis four. 85

Figure 7. Planorbidae richness in ON and SFPE samples for hypothesis four. 86

Figure 8. Hydrobiidae richness in ON and SFPE samples for hypothesis four. 86

Figure 9. Glossiphoniidae richness in ON and SFPE samples for hypothesis four. 87

Figure 10. Brillouin's index for site 1.5U calculated by systematically adding each sample and calculating Brillouin's index, such that the last sample is the sum or composite of all samples. 87

IX Figure 11. Brillouin's index for site. 1.4 calculated by systematically adding each sample and calculating Brillouin's index, such that the last sample is the sum or composite of all samples. 88

Figure 12. Brillouin's index for site New calculated by systematically adding each sample and calculating Brillouin's index, such that the last sample is the sum or composite of all samples. 88

Figure 13. Brillouin's index for site 1.50 calculated by systematically adding each sample and calculating Brillouin's index, such that the last sample is the sum or composite of all samples. 89

Figure 14. Brillouin's index for site 1.3 calculated by systematically adding each sample and calculating Brillouin's index, such that the last sample is the sum or composite of all samples. 89

Figure 15. Brillouin's index for site 1.2 calculated by systematically adding each sample and calculating Brillouin's index, such that the last sample is the sum or composite of all samples. 90

Figure 16. Brillouin's index for site 1 calculated by systematically adding each sample and calculating Brillouin's index, such that the last sample is the sum or composite of all samples. 90

Figure 17. Brillouin's index at site 2, calculated by systematically adding each sample and calculating Brillouin's index, such that the last sample is the sum or composite of all samples. 91

Figure 18. Brillouin's index for site 1.1 calculated by systematically adding each sample and calculating Brillouin's index, such that the last sample is the sum or composite of all samples. 91

Chapter 4

Figure 1. A map of Hardwood Creek and the 9 sample sites. Hardwood Creek flows from Rice Lake into Peltier Lake. 109

Chapter 5

Figure 1. Ranatra sp. presence (1) and absence (0). 129

Figure 2. Stenelmis sp. presence (1) and absence (0). 129

Figure 3. Sperchopsis sp. presence (1) and absence (0). 130

Figure 4. Pseudosmittia sp. 1 presence (1) and absence (0). 130

Figure 5. Cladopelma sp. 1 presence (1) and absence (0). 131

Figure 6. Dicrotendipes sp. 4 presence (1) and absence (0). 131

X Figure 7. Einfeldia sp. gr. 0 presence(1) and absence(O). 132

Figure 8. Stictochironomus sp. presence (1) and absence (0). 132

Figure 9. Similarity indices for recently (R), intermediate (1), and not (N) disturbed hydroseres using ON, ON larvae, and SFPE data sets. 133

Figure 10. Similarity indices across recently (R), intermediate (I), and not (N) disturbed hydroseres using ON, ON larvae, and SFPE data sets. 133

Figure 11. Similarity indices for upstream (US), downstream (OS), and across (AC) sites using ON, ON larvae, and SFPE data sets. 134

Chapter 7

Figure 1. A map of Minnesota, USA, the location of Hardwood Creek, and the 8 sample sites. 157

Appendix A

Figure 1. A generalized cephalothorax of a chironomid pupa and its associated characters. 159

Figure 2. A generalized frontal apotome of a chironomid pupa and its associated characters. 159

Figure 3. Generalized respiratory organs of chironomid pupae and their associated characters. 160

Figure 4. A generalized abdomen of a chironomid pupa and its associated characters. 161

Figure 5. Anal lobe of Orothocladiinae. 165

Figure 6. Anal lobe of Nanocladius sp. 165

Figure 7. Conjunctives of Thienemanniella sp. 165

Figure 8. Conjunctives of Corynoneura sp. 165

Figure 9. Pearl rows of Corynoneura sp. 166

Figure 10. Segment II with pedes spurii B of Nanocladius rectinervis. 166

Figure 11. Tergite IV of Nanocladius rectinervis. 166

Figure 12. Tergites IV-VI of Psectrocladius schlienzi type. 166

Figure 13. Precorneal setae of Nanocladius sp. 166

XI Figure 14. Precorneal setae of some Orthocladiinae. 166

Figure 15. Respiratory organ of Doncricotopus bicaudatus. 166

Figure 16. Anal lobe of Doncricotopus sp. 166

Figure 17. Respiratory organ of Nanocladius crassicornus. 167

Figure 18. Respiratory organ of Nanocladius re ctinervis. 167

Figure 19. Segment II (top) and VIII (bottom) of Parametriocnemus sp. 167

Figure 20. Segment II (top) and VIII (bottom) of Rheocricotopus sp. 167

Figure 21. Anal lobe of Tvetenia sp. 1. 167

Figure 22. Frontal setae of Tvetenia sp. 1. 167

Figure 23. Hook rows on tergites 111-V of Eukiefferiel/a sp. 2. 168

Figure 24. Tergites III-IV of Limnophyes sp. 3. 168

Figure 25. Anal lobe of Diplocladius sp. 168

Figure 26. Tergite II of Chaetocladius sp. 168

Figure 27. Respiratory organ of Acricotopus nitidel/us. 168

Figure 28. Tergite IV of Acricotopus nitidellus. 168

Figure 29. Hook row on segment II of Cricotopus sp. 5. 168

Figure 30. Hook row on segment II of Orthocladius oliveri. 168

Figure 31. Anal lobe of Paraphaenocladius exagitans. 169

Figure 32. Anal lobe of Hydrobaenus sp. 169

Figure 33. Conjunctives VNI-VINII of Pseudosmittia sp. 2. 169

Figure 34. Antepronotal seta of Smittia sp. 169

Figure 35. Antepronotal setae of Pseudosmittia sp. 169

Figure 36. Anal lobe macrosetae of Chaetocladius sp. 170

Figure 37. Tergites III-IV of Cricotopus bicinctus. 171

Figure 38. Dorocentral setae of Cricotopuspolaris. 171

Xll Figure 39. Respiratory organ, precorneal setae, and bumps/wort-like projections of Cricotopus sylvestris. 171

Figure 40. Tergite V of Cricotopus tibialis. 172

Figure 41. Tergite V of Cricotopus (lsocladius) sp. 1. 172

Figure 42. Respirtaory organ of Cricotopus sp. 5. 172

Figure 43. Tergites Ill-VI of Doncricotopus bicaudatus. 173

Figure 44. Respiratory organ of Eukiefferiella sp. 2. 173

Figure 45. Respiratory organ of Nanocladius distinctus. 17 4

Figure 46. Tergites V-VIII of Nanocladius incomptus. 174

Figure 47. Respiratory organ of Nanocladius spiniplenus. 175

Figure 48. Tergite IV of Orthocladius clarkei. 175

Figure 49. Respiratory organ of Orthocladius nigritus. 175

Figure 50. Anal lobe spurs of Orthocladius obumbratus. 176

Figure 51. Respiratory organ of Orthocladius obumbratus. 176

Figure 52. Chitinous threads on the anal lobe of Orthocladius oliveri. 176

Figure 53. Tergites II-IV of Parakiefferiella sp. 7. 177

Figure 54. Tergites III-IV of Parametriocnemus sp. 1. 177

Figure 55. Tergites IV-VI of Parametriocnemus sp. 4. 177

Figure 56. Nose on wing sheath of Paraphaenocladius exagitans. 178

Figure 57. Tergites V-VII of Pseudosmittia sp. 1. 178

Figure 58. Tergite VIII and anal lobe of Rheosmittia sp. 179

Figure 59. Tergites VI-VIII and anal lobe of Th ienemanniella boltoni. 179

Figure 60. Tergite VII of Thienemanniella sp. 1. 180

Figure 61. Respiratory organ of Tvetenia sp. 1 (cf. paucunca). 180

Figure 62. Tergites 11-V of Zavreliella sp. 183

Figure 63. Anal lobe of Endochironomus nigricans. 183

Xlll Figure 64. Anal lobe of C/adopelma sp. 1. 183

Figure 65. Tergite Vof Demeijerea sp. 183

Figure 66. Mace on tergite VI of G/yptopendipes sp. 1. 183

Figure 67. Posterior spine mound on tergite VI of C/adope/ma sp. 1. 183

Figure 68. Conjunctives 1/11-111/IV of Phaenopsectra sp. 1. 183

Figure 69. Cephalic tubercles of Phaenopsectra sp. 183

Figure 70. Anal lobe of Cryptochironomus sp. 184

Figure 71. Respiratory organ of Cryptotendipes sp. 184

Figure 72. Respiratory organ of Polypedilum sca/aenum gr. sp. 8. 184

Figure 73. Respiratory organ of Chironomus sp. 1. 184

Figure 74. Frontal warts of Microtendipes sp. 184

Figure 75. Wing sheath nose of Paralauterbornie/la sp. 184

Figure 76. Segments VII-VIII and anal lobe of Po/ypedilum sp. 184

Figure 77. Tergite IV of Polypedilum obtusum. 184

Figure 78. Tergite Ill and part of II of Paratendipes sp. 1. 185

Figure 79. Respiratory organ of Stenochironomus sp. 185

Figure 80. Segment II (top) and segment IV (bottom) of some Chironomini. 185

Figure 81. Tergites II-III of Harnischia sp. 185

Figure 82. Tergite V of Parachironomus sp. gr. C. 185

Figure 83. Basal ring of Einfeldia sp. gr. D. 185

Figure 84. Basal ring of Chironomus sp. 1. 185

Figure 85. Anal lobe and spurs of Chironomus sp. 185

Figure 86. Anal spurs of Dicrotendip es sp. 6. 186

Figure 87. Bulbous projection on tergite VI of Parachironomus arcuatus gr. 186

Figure 88. Cephalic tuberacles and frontal warts of Chironomus sp. 2. 186

XIV Figure 8�. Segment II of Cladopelma sp. 1. 186

Figure 90. Segment II of C/adopelma sp. 2. 187

Figure 91. Cephalic tuberacles of Cryptochironomus eminentia. 187

Figure 92. Cephalic tuberacles of Cryptochironomus ponderosus. 187

Figure 93. Tergites 1-11 of Cryptochironomus sp. 1 (nr. conus). 188

Figure 94. Tergite Ill of Cryptochironomus sp. 2. 188

Figure 95. Tergite VI shagreen of Dicrotendipes modestus/neomodestus. 189

Figure 96. Tergite VI shagreen of Dicrotendipes nervosus gr. 189

Figure 97. Tergite IV of Dicrotendipes sp. 4. 190

Figure 98. Tergite VI shagreen of Dicrotendipes sp. 6. 190

Figure 99. Tergite Ill of Einfeldia sp. gr. D. 190

Figure 100. Anal comb of Microtendipes sp. 3. 191

Figure 101. Lateral reticulation on part of tergite VII of Parachironomus fre quens gr. 191

Figure 102. Segment II of Parachironomus vitiosus gr. 192

Figure 103. Four, taeniate, lateral setae on segment VII I of Parachironomus sp. gr. C. 192

Figure 104. Tergite II of Parac/adopelma nereis. 192

Figure 1 05. Tergite II of Paratendipes sp. 1. 193

Figure 106. Tergites 11-111 of Paratendipes (?) sp. 2. 193

Figure 107. Tergite Ill shagreen of Phaenopsectra sp. 1. 194

Figure 108. Tergite Ill shagreen of Phaenopsectra sp. 2. 194 ·

Figure 109. Tergites IV-V of Polypedilum fa /lax. 195

Figure 110. Tergite IV and conjunctive spunules of segments 111/IV-IVN of Po/ypedilum illinoense/angulum. 195

Figure 111. Tergites II-III of Polypedilum sca/aenum gr. sp. 1. 196

Figure 112. Tergite Ill of Polypedilum scalaenum gr. sp. 8. 196

XV Figure 113. Cephalic tuberacles and frontal.warts of Polypedilum simulans. 196

Figure 114. Cephalic tuberacles of Po/ypedilum sp. 4. 197

Figure 115. Tergites 111-V of Saetheria ty/us. 197

Figure 116. Tergites III-IV of Saetheria sp. 1. 198

Figure 117. Tergite II of Stenochironomus sp. 3. 198

Figure 118. Tergite IV of Stictochironomus sp. 198

Figure 119. Segment I of Pseudochironomus richardsoni. 199

Figure 120. Respiratory organ of Pseudochironomus sp. 199

Figure 121. Segment VIII and anal lobe of Zavrelia sp. 200

Figure 122. Segment IV of some Tanytarsini. 200

Figure 123. Anal comb and anal lobe of Tanytarsus confusus. 200

Figure 124. Tergite Ill of Rheotanytarsus distinctissimus. 200

Figure 125. Tergites 11-111 of Zavrelia sp. 201

Figure 126. Tergite IV of Paratanytarsus sp. 2. 201

Figure 127. Tergites III-IV and part of V of Micropsectra nigripila. 201

Figure 128. Tergite V of Cladotanytarsus sp. 1. 201

Figure 129. Respiratory organ of C/adotanytarsus sp. 201

Figure 130. Tergites 111-V and part of VI of Ta nytarsus confusus. 201

Figure 131. Tergites 11-V of Cladotanytarsus sp. 1. 202

Figure 132. Respiratory organ of Cladotanytarsus sp. 2. 202

Figure 133. Tergites III-IV and part of tergites II and V of Micropsectra polita. 203

Figure 134. Tergites III-IV and part of tergites II and V of Paratanytarsus inopertus gr. sp. 1. 203

Figure 135. Tergites 111-V of Paratanytarsus inopertus gr. sp. 3. 204

Figure 136. Tergites Ill-VI of Paratanytarsus inopertus gr. sp. 4. 204

Figure 137. Tergites 11-V of Paratanytarsus (cf. /accophilus). 204

XVI Figure 138. Setal sockets of dorsocentral setae 1-4 of Paratanytarsus (cf. laccophilus). 204

Figure 139. Tergites II I-V and part of VI of Paratanytarsus /accophi/us gr. sp. 1. 205

Figure 140. Tergites IV-VI of Paratanytarsus sp. 2. 205

Figure 141. Tergite II of Reotanytarsus distinctissimus. 206

Figure 142. Respiratory organ of Rheotanytarsus sp. 1. 206

Figure 143. Shagreen on tergites II-III of Stempellinella sp. 206

Figure 144. Tergites II-VI of Tanytarsus lobiger. 207

Figure 145. Tergites 111-V and part of VI of Tanytarsus neof/avel/us. 208

Figure 146. Tergite VIII of Ta nytarsus neoflavellus. 208

Figure 147. Tergites II-VI of Tanytarsus sepp. 208

Figure 148. Tergites III-IV and part of V of Tanytarsus wirthi. 209

Figure 149. Tergites 111-V and part of II and VI of Ta nytarsus sp. 1 (cf. gregaris). 209

Figure 150. Tergites II-VI of Tanytarsus sp. 10. 210

Figure 151. Tergites 111-V of Tanytarsus sp. 11. 210

Figure 152. Tergites II-VI of Tanytarsus sp. 12. 211

Figure 153. Tergites Ill-VI of Tanytarsus sp. 16. 211

Figure 154. Tergites II-IV and part of V of Tanytarsus sp. 19. 212

Figure 155. Tergites 111-V and part of VI of Tanytarsus sp. 21. 212

Figure 156. Tergites II-IV and part of V of Tanytarsus sp. 22. 213

Figure 157. Tergites III-IV and part II and V of Tanytarsus sp. 24. 213

Figure 158. Anal lobe of Procladius sub/etteildenticulatus. 215

Figure 159. Anal lobe of Tanypus sp. 215

Figure 160. Tergite I of Ablabesmyia mallochi. 215

Figure 161. Lateral setae on segments I-VII of Natarsia sp. 215

XVll Figure 162. Respiratory organ, basal lobe, and thoracic comb of Ablabesmyia mallochi. 216

Figure 163. Anal lobe and part of segment VIII of Guttipe/opia sp. 216

Figure 164. Basal lobe, postnatal tubercle, and respiratory organ of Guttipelopia sp. 216

Figure 165. Anal lobe and part of segment VIII of Ablabesmyia monilis. 216

Figure 166. Anal lobe and part of segment VIII of Labrundinia pilosella. 216

Figure 167. Respiratory organ, basal lobe, and thoracic comb of Labrundinia pilose/fa. 216

Figure 168. Respiratory organ of Conchape/opia rurika. 217

Figure 169. Shagreen spinules of Conchapelopia sp. 217

Figure 170. Anal lobe of Nilotanypus fimbriatus. 217

Figure 171. Anal lobe of Zavrelimyia sp. 217

Figure 172. Respiratory organ of Zavrelimyia sp. 217

Figure 173. Respiratory oragan of Paramerina sp. 217

Figure 174. Respiratory organ of Larsia sp. 218

Figure 175. Shagreen spinules of Larsia sp. ·218

Figure 176. Wing pad of Ab/abesmyia mallochi. 218

Figure 177. Wing pad of Ablabesmyia monilis. 219

Figure 178. Respiratory organ and basal lobe of Conchapelopia dusena. 219

Figure 179. Respiratory organ and tubercle of Natarsia sp. 220

Figure 180. Anal lobe of Proc/adius bel/us. 221

Figure 181. Anal lobe of Procladius freemani. 221

Figure 182. Slightly damaged respiratory organ of Tanypus sp. 221

Appendix 8

Chapter 3

Figure 1. Ephemeroptera richness in DN and SFPE samples. 226

XVlll Figure 2. Odonata richness in ON and SFPE samples. 226

Figure 3. Plecoptera richness in ON and SFPE samples. 227

Figure 4. Hemiptera richness in ON and SFPE samples. 227

Figure 5. Megaloptera richness in ON and SFPE samples. 227

Figure 6. Trichoptera richness in ON and SFPE samples. 228

Figure 7. Lepidoptera richness in ON and SFPE sample�. 228

Figure 8. Coleoptera richness in ON and SFPE samples. 228

Figure 9. Oiptera richness in ON and SFPE samples. 229

Figure 10. Nematoda richness in ON and SFPE samples. 229

Figure 11. Veneroida richness in ON and SFPE samples. 229

Figure 12. Basommatophora richness in ON and SFPE samples. 230

Figure 13. Heterostropha richness in ON and SFPE samples. 230

Figure 14. Arhynchobdellida richness in ON and SFPE samples. 230

Figure 15. Oligochaeta richness in ON and SFPE samples. 231

Figure 16. lsopoda richness in ON and SFPE samples. 231

Figure 17. Amphipoda richness in ON and SFPE samples. 231

Figure 18. Oecapoda richness in ON and SFPE samples. 232

Figure 19. Baetidae richness in ON and SFPE samples. 232

Figure 20. Caenidae, Pleidae, Oligochaeta, and Hyalellidae richness in ON and SFPE samples. 232

Figure 21. Heptageniidae and Perlidae richness in ON and SFPE samples. 233

Figure 22. Leptophlebiidae, Corydalidae, and Oolichipodinae richness in ON and SFPE samples. 233

Figure 23. Calopterygidae richness in ON and SFPE samples. 233

Figure 24. Coenagrionidae richness in ON and SFPE samples. 234

Figure 25. Libellulidae richness in ON and SFPE samples. 234

XIX Figure 26. Capniidae, Nemouridae, Glossosomatidae, and Hydropsychidae · richness in DN and SFPE samples. 234

Figure 27. Belostomatidae richness in DN and SFPE samples. 235

Figure 28. Corixidae richness in DN and SFPE samples. 235

Figure 29. Gerridae richness in DN and SFPE samples. 235

Figure 30. Hebridae richness in DN and SFPE samples. 236

Figure 31. Hydrometridae richness in DN and SFPE samples. 236

Figure 32. Mesoveliidae and Pyralidae richness in DN and SFPE samples. 236

Figure 33. Nepidae and Pseudochironomini richness in DN and SFPE samples. 237

Figure 34. Notonectidae richness in DN and SFPE samples. 237

Figure 35. Veliidae, Scirtidae, and Asellidae richness in DN and SFPE samples. 237

Figure 36. Hydroptilidae richness in DN and SFPE samples. 238

Figure 37. Leptoceridae richness in DN and SFPE samples. 238

Figure 38. Limnephilidae richness in DN and SFPE samples. 238

Figure 39. Curculionidae richness in DN and SFPE samples. 239

Figure 40. Elmidae richness in DN and SFPE samples. 239

Figure 41. Haliplidae richness in DN and SFPE samples. 239

Figure 42. Hydraenidae richness in DN and SFPE samples. 240

Figure 43. Hydrophilidae richness in DN and SFPE samples. 240

Figure 44. Ceratopogonidae richness in DN and SFPE samples. 240

Figure 45. Chironomidae richness in DN and SFPE samples. 241

Figure 46. Chironomini richness in DN and SFPE samples. 241

Figure 47. Orthocladiinae richness in DN and SFPE samples. 241

Figure 48. richness in DN and SFPE samples. 242

Figure 49. Tanypodinae richness in DN and SFPE samples. 242

Figure 50. Tanytarsini richness in DN and SFPE samples. 242

XX Figure 51. Culicid�e richness in ON and SFPE samples. 243

Figure 52. Oixidae richness in ON and SFPE samples. 243

Figure 53. Empididae richness in ON and SFPE samples. 243

Figure 54. Ephydridae richness in ON and SFPE samples. 244

Figure 55. Psychodidae richness in ON and SFPE samples. 244

Figure 56. Sciomyzidae richness in ON and SFPE samples. 244

Figure 57. Simuliidae richness in ON and SFPE samples. 245

Figure 58. Stratiomyidae richness in ON and SFPE samples. 245

Figure 59. Syrphidae richness in ON and SFPE samples. 245

Figure 60. Tabanidae richness in ON and SFPE samples. 246

Figure 61. Tipulidae richness in ON and SFPE samples. 246

Figure 62. Nematoda richness in ON and SFPE samples. 246

Figure 63. Pisidiidae richness in ON and SFPE samples. 24 7

Figure 64. Ancylidae and Gammaridae richness in ON and SFPE samples. 247

Figure 65. Lymnaeidae richness in ON and SFPE samples. 247

Figure 66. Physidae richness in ON and SFPE samples. 248

Figure 67. Valvatidae richness in ON and SFPE samples. 248

Figure 68. Erobdellidae richness in ON and SFPE samples. 248

Figure 69. Cambaridae richness in ON and SFPE samples. 249

Chapter 5

Figure 70. Baetis sp. presence and absence. 250

Figure 71. Callibaetis sp., Paratanytarsus inopertus gr. sp. 3, and Valvata tricarinata presence and absence. 250

Figure 72. Labiobaetis sp., Rheotanytarsus sp. 1, Parakiefferiella sp. 7, Orthocladius obumbratus, Orthocladius clarkei, Orconectes virilis, and Mallochohelea sp. presence and absence. 251

XXI Figure 73. Caenis sp., Trichocorixa sp., Neoplea sp., .Liodessus sp., Odontomyia sp., Pisidium sp., Physella gyrina, undetermined Oligochaeta, Hyallela azteca, Limnophyes sp. 3, Micropsectra nigripila presence and absence. 251

Figure 74. Heptagenia sp., Stenochironomus sp. 3, Saetheria tylus, Paracladopelma nereis, Harnischia sp., Tvetenia sp. 1 (cf. paucunca), Rheocricotopus (Psilocricotopus) sp. 1, Eukiefferiel/a sp. 2, Hemerodromia sp., Dubiraphia sp., Rheumatobates sp., and Perlesta sp. presence and absence. 252

Figure 75. Habrophlebiodes sp., Dicrotendip es nervosus gr., Dicrotendipes modestus/neomodestus, Parametriocnemus sp. 4, Nephe/opsis obscura, undetermined Dolichipodidae, Listronotus angustus, and Chauliodes sp. presence and absence. 252

Figure 76. Calopteryx sp., Ab/abesmyia mallochi, Tanytarsus sp. 21, Ta nytarsus sp. 16, Rheotanytarsus distinctissimus, Dicrotendipes sp. 6, Cryptochironomus ponderosus, Rheosmittia sp., Orthocladius trigonolabis, Orthoc/adius nigritus, and Hydroporus sp. presence and absence. 253

Figure 77. Coenagrion/Enallagma sp. presence and absence. 253

Figure 78. /schnura sp. and Leptocerus sp. presence and absence. 253

Figure 79. Somatochlora sp. presence and absence. 254

Figure 80. Undetermined Capniidae, Conchapelopia dusena, Polyp edilum obtusum, Smittia sp., Nanocladius spiniplenus, Nanocladius crassicornus, Hydrobaenus sp., Che/ifera sp., Optioservus sp., Cheumatopsyche sp., undetermined Glossosomatidae, and undetermined Nemouridae presence and absence. 254

Figure 81. Be/ostoma sp., Tanytarsus sp. 24, Tanytarsus sp. 11, Ta nytarsus wirthi, Tanytarsus neoflavellus, Pseudochironomus richardsoni, Parachironomus sp. gr. C, and Promenetus exacuous presence and absence. 255

Figure 82. Sigara sp. and Tanytarsus sp. 22 presence and absence. 255

Figure 83. Gerris sp. presence and absence. 255

Figure 84. Merragata sp. presence and absence. 256

Figure 85. Hydrometra sp., Zavrelimyia sp., Parachironomus arcuatus gr., Alboglossiponia heteroclite, Gyrau/us deflectus, He/ius sp., Psychoda sp., Hydrovatus sp., Triaenodes sp., and Orthotrichia sp. presence and absence. 256

Figure 86. Mesove/ia sp., Sync/ita sp., Peltodytes sp., Nanoc/adius rectinervis, and Ablabesmyia monilis presence and absence. 256

Figure 87. Notonecta sp. and Oxyetheria sp. presence and absence. 257

XXll Figure 88. Microvelia sp., Bezzia/Palpomyia sp., Scirtes sp., Oecetis sp., Helobdella stagnalis, Caecidotea sp., and Thienemanniella lobapodema presence and absence. 257

Figure 89. Agraylea sp., Paratanytarsus laccophilus gr. sp. 1, and Desserobdel/a michiganensis presence and absence. 257

Figure 90. Nectopsyche sp. and Tanytarsus lobiger presence and absence. 258

Figure 91. Asynarchus sp., Paratanytarsus sp. 2, and Antichaeta sp. presence and absence. · 258

Figure 92. Lissorhpoterus simplex and Al/uaudomyia sp. presence and absence. 258

Figure 93. Tanysphyrus lemnae presence and absence. 259

Figure 94. Agabus sp., Nilotanypus fimbriatus, Saetheria sp. 1, and Phaenopsectra sp. 2 presence and absence. 259

Figure 95. Hygrotus sp., Micropsecfra polita , and Helisoma anceps presence and absence. 259

Figure 96. Hygrotus!Hydroporus sp., Paratanytarsus (ct. laccophilus), Parachironomus vitosus gr. , Parachironomus varus gr., Dicrotendipes tritomus, Pseudosmittia sp. 2, Culiseta inornata, Setacera sp., Corynoneura sp. 2, and Polyp edilum trigonus presence and absence. 260

Figure 97. 1/ybius sp. and Amnicola walkeri presence and absence. 260

Figure 98. Laccophilus sp. presence and absence. 260

Figure 99. Neoporus sp. and Procladius fre emani presence and absence. 261

Figure 100. Haliplus sp. presence and absence. 261

Figure 101. Hydraena sp. and Paratendipes sp. 1 presence and absence. 261

Figure 102. Anacaena sp. presence and absence. 262

Figure 103. Berosus sp. and Glyptotendipes sp. 1 presence and absence. 262

Figure 104. Cymbiodyta sp., Guttipelopia sp., Stempellinella sp., Paratanytarsus inopertus gr. sp. 4, Zavreliel/a sp., Zavrelia sp., Polypedilum simulans, Paratendipes (?) sp. 2, Eristalis sp., Stratiomys sp., Dasyhelea sp., and Atrichopogon sp. presence and absence. 262

Figure 105. Enochrus sp. presence and absence. 263

Figure 106. Helophorus sp. presence and absence. 263

XXlll Figure 107. Hydrobius sp., Polypedilum scalaenum gr. sp. 8, and Polypedilum fa /lax presence and absence. 263

Figure 108. Hydrochus sp. presence and absence. 264

Figure 109. Paracymus sp., Physel/a integra, and Pseudosuccinea columella presence and absence. 264

Figure 110. Tropisternus sp. and Th ienemanniel/a simi/is presence and absence. 264

Figure 111. Clinohelea!Heteromyia sp. and Gyraulus parvus presence and absence. 265

Figure 112. Aedes cinereus presence and absence. 265

Figure 113. Aedes vexans, Larsia sp., and Chironomus sp. 1 presence and absence. 265

Figure 114. Anopheles earlei presence and absence. 266

Figure 115. Anopheles punctipennis and Cladotanytarsus sp. 1 presence and absence. 266

Figure 116. Culex territans presence and absence. 266

Figure 117. Dixella sp. presence and absence. 267

Figure 118. Ephydra sp. presence and absence. 267

Figure 119. Hydrellia sp. presence and absence. 267

Figure 120. Pericoma sp. presence and absence. 268

Figure 121. Simulium sp. presence and absence. 268

Figure 122. Odontomyia/Hedriodiscus sp. and Fossaria modicella presence and absence. 268

Figure 123. Oxycera sp., Polypedilum sp. 4, Polypedilum (nr. falciforme), Microtendipes sp. 3, Demeijeria sp., Cricotopu"spolar is, and Prionocera sp. presence and absence. 269

Figure 124. Chrysops sp. presence and absence. 269

Figure 125. Limnophila sp. presence and absence. 269

Figure 126. Nematoda and Planorbella trivolvis presence and absence. 270

Figure 127. Musculium sp. presence and absence. 270

XXIV Figure 128. Sphaerium sp. presence and absence. 270

Figure 129. Ferrissia tragi/is, Cryptochironomus eminentia, and Gammarus sp. presence and absence. 271

Figure 130. Stagnicola caperata and Chaetocladius sp. (weak, elongate AMS) presence and absence. 271

Figure 131. Stagnicola elodes presence and absence. 271

Figure 132. Aplexa elongata presence and absence. 272

Figure 133. Planorbula armigera, Th ienemanniella boltoni, Psectrocladius schlienzi type, and Cricotopus sylvestris presence and absence. 272

Figure 134. Erpobdella punctata presence and absence. 272

Figure 135. G/ossiphonia complanata presence and absence. 273

Figure 136. He/obdella fu sca presence and absence. 273

Figure 137. Placobdel/a papillfera and Cricotopus tibialis presence and absence. 273

Figure 138. Theromyzon biannulatum, Phaenopsectra sp. 1, Cryptotendipes sp., Nanocladius incomptus, and Diplocladius sp. presence and absence. 274

Figure 139. Acricotopus nitidellus presence and absence. 27 4

Figure 140. Corynoneura sp. 1 presence and absence. 27 4

Figure 141. Cricotopus bicinctus and Nanocladius distinctus presence and absence. 275

Figure 142. Cricotopus (lsocladius) sp. 1 and Doncricotopus bicaudatus presence and absence. 275

Figure 143. Cricotopus sp. 5 presence and absence. 275

Figure 144. Orthoc/adius oliveri and Polypedilum illinoense presence and absence. 276

Figure 145. Parametriocnemus sp. 1 presence and absence. 276

Figure 146. Paraphaenocladius exagitans and Tanytarsus sp. 1 (cf. gregarius gr. ) presence and absence. 276

Figure 147. Th ienemanniella sp. 1 presence and absence. 277

Figure 148. Chironomus sp. 2 presence and absence. 277

XXV Figure 149. Cladopelma sp. 2 presence and absence. 277

Figure 150. Cryptochironomus sp. 1 (nr. co nus) presence and absence. 278

Figure 151. Cryptochironomus sp. 2 presence and absence. 278

Figure 152. Endochironomus nigricans presence and absence. 278

Figure 153. Parachironomus frequens gr. presence and absence. 279

Figure 154. Paralauterborniel/a nigrohalterale, Labrundinia pilosella, Tanytarsus confusus, and Po/ypedilum scalaenum gr. sp. 1 presence and absence. 279

Figure 155. C/adotanytarsus sp. 2 and Natarsia sp. presence and absence. 279

Figure 156. Paratanytarsus inopertus gr. sp. 1 and Ta nytarsus sp. 10 presence and absence. 280

Figure 157. Ta nytarsus sepp presence and absence. 280

Figure 158. Tanytarsus sp. 12 presence and absence. 280

Figure 159. Ta nytarsus sp. 19 presence and absence. 281

Figure 160. Conchapelopia rurika presence and absence. 281

Figure 161. Paramerina sp. presence and absence. 281

Figure 162. Procladius bel/us presence and absence. 282

Figure 163. Procladius sublettei/denticulatus presence and absence. 282

Figure 164. Tanypus sp. presence and absence. 282

Figure 165. Endotribelos sp. larvae presence and absence. 283

Figure 166. Microchironomus sp. larvae presence and absence. 283

Figure 167. Cricotopus trifascia and Clinotanypus sp. larvae presence and absence. 283

Figure 168. Stackelbergina sp. larvae presence and absence. 284

Figure 169. Stilocladius sp. larvae presence and absence. 284

Figure 170. Odontomesa sp. larvae presence and absence. 284

Figure 171. Monopelopia sp. larvae presence and absence. 285

Figure 172. Pentaneura sp. larvae presence and absence. 285

XXVI Appendix B

Supplemental Tables and Figures

Introduction

The following figures and tables were produced to test the hypotheses presented in the previous chapters.

Chapter 2

Tables

SMC SMC SMC JAC JAC JAC WPS WPS WPS Method mean min. max. mean min. max. mean min. max. ON 0.631 0.484 0.770 0.398 0.177 0.613 0.418 0.163 0.694 L 0.599 0.353 0.804 0.408 0.115 0.706 0.336 0.071 0.717 SFPE 0.585 0.388 0.783 0.294 0.133 0.594 * * *

Table 1. The mean, minimum (min.), and maximum (max.) similarity index values calculated for SMC, JAC, and WPS indices for all possible site combinations. Calculations were made for DN, chironomid larvae (L), and SFPE data sets. An asterisk (*) indicates where an index value was not calculated.

Correlation coefficients SMC JAC WPS DN/L 0.661 0.769 0.532 DN/SFPE 0.779 0.735 *

LISFPE 0.812 0.658 *

Table 2. The correlation coefficients based on similarity index values calculated for all site comparisons for the DN and larvae (DN/L), DN/SFPE, and larvae and SFPE (L/SFPE) comparisons. An asterisk (*) indicates where an index value was not calculated.

225 Chapter 3

Figures

Order richness for ON and SFPE samples

6 5 en en Q) 4 c ...c (.) 3 ·c ro X 2 ro I- 1

--- 0 I 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 1. Ephemeroptera richness in ON and SFPE samples.

4

en en 3 Q) c ...c (.) ·c 2 .co X ro I- 1

0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 2. Odonata richness in ON and SFPE samples.

226 4

en en 3 Q) c ..c (.) 2 ·c co >< 1-co 1

0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 3. Plecoptera richness in DN and SFPE samples.

12 10 en en Q) 8 c ..c (.) 6 ·c co >< 4 1-co 2

-- 0 ----- r------r ------r - - -- T 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 4. Hemiptera richness in DN and SFPE samples.

en en Q) c ..c (.) ·c co >< co 1-

1.5U 1.4 1 . 3 1 . 50 1.1 1.2 2 Site

Figure 5. Megaloptera richness in DN and SFPE samples.

227 6 5 - en en Q) 4 c ..c (,) 3 ·c ro >< 2 ro I- 1 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 6. Trichoptera richness in DN and SFPE samples.

2

en en Q) c ..c

.g 1 • • • • • • ro >< ro I-

-- -- -� -�-�-- --�---� � --- �� ------� 0 1- r T -�r 1 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site·

Figure 7. Lepidoptera richness in DN and SFPE samples.

16 14 en en 12 Q) c ..c (,) ·c 8 ro >< 6 ro I- 4 2 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 8. Coleoptera richness in DN and SFPE samples.

228 80 70 en en 60 Q) c 50 ..c (.) ·c 40 ro - X 30 ro I- 20 10 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 9. Oiptera richness in ON and SFPE samples.

2

en en Q) c ..c (.) ·c 1 ro X ro I-

0 ----r 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 10. Nematoda richness in ON and SFPE samples.

4 I

en en 3 Q) ' c ..c ·c(.) 2 ro 1 X ro I- 1 I 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 11. Veneroida richness in ON and SFPE samples.

229 12

en 10 en Q) c 8 ..c u ·c 6 co X 4 co I- 2 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 12. Basommatophora richness in ON and SFPE samples.

2

en en Q) c ..c u ·c • • • co X co I-

------0 1 r 1.5U 1.4 1.3 1.50 ·1.1 1.2 2 Site

Figure 13. Heterostropha richness in ON and SFPE samples.

3

en en � 2 ..c u ·c co X co I-

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 14. Arhynchobdellida richness in ON and SFPE samples.

230 2 I !I en en Q) c ...c I I {.) I ·c 1 -j • • • • • • • • co I >< co 1- I

0 -t-- 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 15. Oligochaeta richness in DN and SFPE samples. 2 l �w 1 1 I & 0 i- 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 16. lsopoda richness in DN and SFPE samples.

3

en en Q) c 2 ...c {.) ·c co >< 1 co 1-

0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 17. Amphipoda richness in DN and SFPE samples.

231 2

(j) (j) Q) c ..c

·c(.) ro X ro I-

0 1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 18. Oecapoda richness in ON and SFPE samples.

Family richness for DN and SFPE samples

(j) (j) � 2 ..c (.) ·c � 1 ro I-

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 19. Baetidae richness in ON and SFPE samples.

2

(j) (j) Q) c ..c (.) ·c • • • • • • • • ro X ro I-

0 . ·- -- - I r I 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 20. Caenidae, Pleidae, Oligochaeta, and Hyalellidae richness in ON and SFPE samples. 232 2-

en en Q) c ...c (.) ·c • • • ro X ro I-

0 L I • I • • 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 21. Heptageniidae and Perlidae richness in ON and SFPE samples.

2

en en Q) c ...c (.) ·c ro X ro I-

0 -� ----r-+---;--+--..,.-...._.....,-.... 1.5U 1.4 1.3 1.50 1 1.1 1.2 2

Site

Figure 22. Leptophlebiidae, Corydalidae, and Oolichipodinae richness in ON and SFPE samples.

2

en en Q) c ...c (.) ·c ro X ro I-

0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 23. Calopterygidae richness in ON and SFPE samples.

233 3

(/) (/) Q) 2 c ..c (.) ·;:: ro X 1 ro I-

0 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 24. Coenagrionidae richness in ON and SFPE samples.

2

(/) (/) Q) c ..c (.) ·;:: ro X ro I-

0 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 25. Libellulidae richness in ON and SFPE samples.

2l

(/) (/) Q) c ..c (.) ·;:: ro X ro I-

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 26. Capniidae, Nemouridae, Glossosomatidae, and Hydropsychidae richness in ON and SFPE samples.

234 2

CJ) CJ) : Q) c

..c I () 1 � ·c • co X co I- I \!\ 0 • • I • 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 27. Belostomatidae richness in DN and SFPE samples.

3

CJ) CJ) Q) 2 c ..c

·c() co X co I-

0 ------T 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 28. Corixidae richness in DN and SFPE samples.

3

CJ) CJ) Q) 2 c ..c

·c() co X 1 co I-

0 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 29. Gerridae richness in DN and SFPE samples.

235 2

en en Q) c ..c. u ·c 1 ro X ro 1-

0 1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 30. Hebridae richness in ON and SFPE samples.

2

en en Q) c ..c. u ·c 1 ro X ro 1-

0 1.5U 1.4 1.3 1.50 1 1.1 1.2 2

Site

Figure 31. Hydrometridae richness in ON and SFPE samples.

2

en en Q) c ..c. u 1 ·c • • • • • • ro X ro 1-

0 � 1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 32. Mesoveliidae and Pyralidae richness in ON and SFPE samples.

236 2

(/) (/) 1 Q) c ..c u 1 ·c • co X co r

0 ---r 1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 33. Nepidae and Pseudochironomini richness in ON and SFPE samples.

2

(/) (/) Q) c ..c u ·c co X co r

0 1. 5U 1.4 1. 3 1. 50 1.1 1.2 2

Site

Figure 34. Notonectidae richness in ON and SFPE samples. 2 1 (/) (/) Q) c ..c u ·c • • • • • co X co r \! 0 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 35. Veliidae, Scirtidae, and Asellidae richness in ON and SFPE samples.

237 3

CJ) CJ) Q) 2 c ..c u ·c co X co I-

0 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 36. Hydroptilidae richness in DN and SFPE samples.

4

� 3 Q) c ..c .g 2 co X co I-

- --1 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 37. Leptoceridae richness in DN and SFPE samples.

2

CJ) CJ) Q) c ..c u ·c • • co X co I-

0 i6 j_ 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 38. Limnephilidae richness in DN and SFPE samples.

238 3lI en en � 2- ...c (.) ·c co X co I-

1. 5U 1.4 1. 3 1. 50 1.1 1.2 2

Site

Figure 39. Curculionidae richness in ON and SFPE samples.

4

en en 3 Q) c ...c

·c(.) 2 co X co I-

0 1.5U 1.4 1.3 1.50 1 1.1 1.2 2

Site

Figure 40. Elmidae richness in ON and SFPE samples.

3 � en en Q) 2 - c ...c (.) ·c co X 1 co I-

0 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 41. Haliplidae richness in ON and SFPE samples.

239 2

(/) (/) Q) c ..c u 1 ·c • • • • • • co X co I-

0 l 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 42. Hydraenidae richness in ON and SFPE samples.

8 7 (/) (/) 6 Q) c 5 ..c u 4 ·c co 3 X co I- 2 1 0 - ----, 1.5U 1.4 1.3 1.50 1 1.1 1.2 2

Site

Figure 43. Hydrophilidae richness in ON and SFPE samples.

5

(/) 4 (/) Q) c ..c 3 u ·c co 2 - X co I-

0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 44. Ceratopogonidae richness in ON and SFPE samples.

240 70

60 en en 50 Q) c ..c 40 () ·c 30 co X co 20 . � 10 .

0 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 45. Chironomidae richness in ON and SFPE samples.

25

en 20 en Q) c ..c 15 () ·c co 10 X co � 5

---· ···-·- · 0 r r 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 46. Chironomini richness in ON and SFPE samples.

25

en 20 en Q) c ..c 15

·c() co 10 - X co � 5

0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 47. Orthocladiinae richness in ON and SFPE samples.

241 en en Q) c ..c (.) ·c co X co 1-

1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 48. Prodiamesinae richness in DN and SFPE samples.

12

10 en en Q) 8 c ..c (.) ·c 6 co X 4 co 1- 2

0 -- - T ---- T -r- - -- I 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 49. Tanypodinae richness in DN and SFPE samples.

18 16 en 14 - en Q) 12 c ..c 10 (.) ·c 8 co X 6 co 1- 4 2 0 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 50. Tanytarsini richness in DN and SFPE samples.

242 5

en 4 en Q) c ..c 3 () ·c m 2 X m 1- 1 -

0 1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 51. Culicidae richness in ON and SFPE samples.

2

en en Q) c ..c

·c() m X m 1-

0 � 1 1.5U 1.4 1.3 1.50 1 1.1 1.2 2

Site

Figure 52. Oixidae richness in ON and SFPE samples.

en en Q) 2 c ..c () ·c m X m 1-

0 1.5U 1.4 1.3 1.50 1 1.1 1.2 2

Site

Figure 53. Empididae richness in ON and SFPE samples.

243 4

en en 3 Q) c .c () ·c 2 ro X ro 1 I-

0 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 54. Ephydridae richness in DN and SFPE samples.

2

en en Q) c .c () ·c ro X ro I-

1- - �------, -- 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 55. Psychodidae richness in DN and SFPE samples.

3

en en Q) 2 c .c () ·c ro X 1 ro I-

0

1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 56. Sciomyzidae richness in DN and SFPE samples.

244 2

CJ) CJ) Q) c ..c (.) ·c 1 co X co �

0 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 57. Simuliidae richness in DN and SFPE samples.

4

� 3 Q) c ..c .g 2 co X co 1 �

------r-- _ T ____ -- -- -,------, 1.5U 1.4 1.3 1.50 1 1.1 1.2 2

Site

Figure 58. Stratiomyidae richness in DN and SFPE samples.

2 l

CJ) CJ) Q) c ..c .g 1 co X co �

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 59. Syrphidae richness in DN and SFPE samples.

245 en en ()) c ..c () ·c co X co I-

1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 60. Tabanidae richness in DN and SFPE samples.

3

en en ()) 2 c ..c () ·c co X co I-

0 ---, 1.5U 1.4 1.3 1.50 1.1 1.2 2

Site

Figure 61. Tipulidae richness in DN and SFPE samples.

en en ()) c ..c () ·c co X co I-

1.5U 1 .4 1. 3 1 .50 1.1 1.2 2

Site

Figure 62. Nematoda richness in DN and SFPE samples.

246 4 I I I (j) (j) 3 Q) c ..c II (.) ·c 2� co i X co 1 � I- I 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 63. Pisidiidae richness in ON and SFPE samples.

2

(j) (j) Q) c ..c (.) ·c

0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 64. Ancylidae and Gammaridae richness in ON and SFPE samples.

4

(j) (j) 3 Q) c ..c (.) ·c 2 co X co I-

0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 65. Lymnaeidae richness in ON and SFPE samples.

247 4

(j) (j) 3 Q) c ..c u ·c 2 I co X co I- 1 1 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 66. Physidae richness in ON and SFPE samples.

2

(j) (j) Q) c ..c u ·c • • co X co I-

0 -- T ---, - 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 67. Valvatidae richness in ON and SFPE samples.

(j) (j) � 2 ..c u ·c co X co I-

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 68. Erobdellidae richness in ON and SFPE samples.

248 2

en en Q) c ..c (.) ·c 1 • • • ro >< I ro I- I 0 I I • I 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 69. Cambaridae richness in DN and SFPE samples.

Chapter 5

Tables

Disturbance/method SMC JAC WPS Averages Recently disturbed 0.691 0.296 0.453 Intermediately disturbed 0.636 0.439 0.450 Not disturbed 0.717 0.525 0.607 Differences Recently - intermediately 0.055 -0.142 0.003 Recently - not -0.026 -0.229 -0.155 Intermediately - not -0.081 -0.087 -0.157

Table 3. The recently, intermediately, and not disturbed hydroseres for DN, chironomid larvae, and SFPE methods. Similarity index values were calculated using SMC, JAC, and WPS indices. Whittaker's index was not calculated for the SFPE method.

Comparison and method SMC JAC WPS Averages Recent/intermediate 0.558 0.275 0.277 Recent/not 0.507 0.212 0.258 Intermediate/not 0.642 0.441 0.421 Differences Rec./int. - Rec./not 0.052 0.064 0.019 Rec./int. - int./not -0.084 -0.166 -0.143 Rec./not - int./not -0.136 -0.229 -0.163

Table 4. The recent and intermediate disturbed, recent and not disturbed, and intermediate and not disturbed for DN, chironomid larvae, and SFPE methods. Similarity index values were calculated using SMC, JAC, and WPS indices. Whittaker's index was not calculated for the SFPE method.

249 Comparison/method SMC JAC WPS Averages Upstream 0.632 0.338 0.322 Downstream 0.71 1 0.543 0.556 Across 0.559 0.325 0.351 Differences Up - down -0.079 -0.205 -0.234 Up - across 0.073 0.013 -0.029 Down - across 0.152 0.218 0.205

Table 5. Upstream, downstream: across upstream and downstream sites, and their average values for ON, chironomid larvae, and SFPE methods. Similarity index values were calculated using SMC, JAC, and WPS similarity indices. Whittaker's index was not calculated for the SFPE method.

Figures

Q) u c Q) en ..c � Q) u c Q) en Q) a..I...

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 70. Baetis sp. presence and absence.

1 Q) u c Q) en ..c co -Q) u c Q) en Q) a..I... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 71. Cal/ibaetis sp., Paratanytarsus inopertus gr. sp. 3, and Valvata tricarinata presence and absence.

250 1 Q) (.) c Q) rn ...Q ro -Q) (.) c Q) rn Q) a..1- 0 l 1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 72. Labiobaetis sp., Rheotanytarsus sp. 1, Parakiefferiel/a sp. 7, Orthocladius obumbratus, Orthocladius clarkei, Orconectes virilis, and Mal/ochohelea sp. presence and absence.

• • • • • • • • Q) (.) c Q) rn ...Q ro -Q) (.) c Q) rn Q) a..s- 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 73. Caenis sp., Trichocorixa sp., Neoplea sp., Liodessus sp., Odontomyia sp., Pisidium sp., Physel/a gyrina, undetermined Oligochaeta, Hyallela azteca, Limnophyes sp. 3, Micropsectra nigripila presence and absence.

251 1 Q) () c Q) en ..0 co -Q) () c Q) en Q) i... a_ 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 74. Heptagenia sp., Stenochironomus sp. 3, Saetheria ty/us, Paracladopelma nereis, Harnischia sp., Tvetenia sp. 1 (cf. paucunca), Rheocricotopus (Psilocricotopus) sp. 1, Eukiefferiella sp. 2, Hemerodromia sp., Dubiraphia sp., Rheumatobates sp., and Perlesta sp. presence and absence.

Q) () c Q) en ..0 � Q) () c Q) en Q) i... a_

1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 75. Habroph/ebiodes sp., Dicrotendipes nervosus gr., Dicrotendipes modestuslneomodestus, Parametriocnemus sp. 4, Nephelopsis obscura, undetermined Dolichipodidae, Listronotus angustus, and Chauliodes sp. presence and absence.

252 Q) (.) c Q) en ..0 ro -Q) (.) c Q) en Q) 0...I....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 76. Calopteryx sp., Ablabesmyia mallochi, Tanytarsus sp. 21, Tanytarsus sp. 16, Rheotanytarsus distinctissimus, Dicrotendipes sp. 6, Cryptochironomus ponderosus, Rheosmittia sp., Orthocladius trigonolabis, Orthocladius nigritus, and Hydroporus sp. presence and absence.

1 Q) (.) c Q) en ..0 ro -Q) (.) c Q) en Q) 0...I.... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 77. Coenagrion!Enallagma sp. presence and absence.

Q) (.) c Q) en ..0 � Q) (.) c Q) en Q) 0...I....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 78. lschnura sp. and Leptocerus sp. presence and absence. 253 Q) () c Q) en ..0 co Q; () c Q) en � 0...

1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 79. Somatochlora sp. presence and absence.

Q) () c Q) en ..0 co Q; () c Q) en Q) 0...I....

- I 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 80. Undetermined Capniidae, Conchape/opia dusena, Polypedilum obtusum, Smittia sp., Nanocladius spiniplenus, Nanoc/adius crassicornus, Hydrobaenus sp., Chelifera sp., Optioservus sp., Cheumatopsyche sp., undetermined Glossosomatidae, and undetermined Nemouridae presence and absence.

254 Q) () c Q) en ..Q co (B () c Q) en � a_

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 81. Belostoma sp., Tanytarsus sp. 24, Tanytarsus sp. 11, Tanytarsus wirthi, Tanytarsus neoflavellus, Pseudochironomus richardsoni, Parachironomus sp. gr. C, and Promenetus exacuous presence and absence.

Q) () c Q) en ..Q � Q) () c Q) en Q) '- a_ 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 82. Sigara sp. and Tanytarsus sp. 22 presence and absence.

Q) () c Q) en ..Q � () c Q) en Q) '- a_ 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 83. Gerris sp. presence and absence.

255 Q) (..) c Q) en ..0 � (..) c Q) en Q) L... a_

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 84. Merragata sp. presence and absence.

Q) (..) c Q) en ..0 � Q) (..) c Q) en � a_ 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 85. Hydrometra sp., Zavrelimyia sp., Parachironomus arcuatus gr., Alboglossiponia heteroclite, Gyrau/us deflectus, He/ius sp., Psychoda sp., Hydro vatus sp., Triaenodes sp., and Orthotrichia sp. presence and absence.

Q) (..) c Q) en ..0 � (..) c Q) en � a_ 0 1- 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 86. Mesovelia sp., Sync/ita sp., Peltodytes sp., Nanocladius rectinervis, and Ablabesmyia moni/is presence and absence. 256 Q) (..) c Q) en ...0 � (..) c Q) en Q) a..I....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 87. Notonecta sp. and Oxyetheria sp. presence and absence.

Q) (..) c Q) en ...0 ro CD (..) c Q) en � a.. 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 88. Microvelia sp., Bezzia/Palpomyia sp., Scirtes sp., Oecetis sp., Helobdella stagnalis, Caecidotea sp., and Thienemanniella /obapodema presence and absence.

1 Q) (..) c Q) en ...0 ro CD (..) c Q) en Q) a..I.... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 89. Agraylea sp., Paratanytarsus laccophilus gr. sp. 1, and Desserobdella michiganensis presence and absence.

257 Q) (.) c Q) C/) .0 � (.) c Q) C/) Q) 0..1.... 0 1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 90. Nectopsyche sp. and Tanytarsus lobiger presence and absence.

Q) (.) c Q) C/) .0 ro (D (.) c Q) C/) � 0..

1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 91. Asynarchus sp., Paratanytarsus sp. 2, and Antichaeta sp. presence and absence.

Q) (.) c Q) C/) .0 ro (D (.) c Q) C/) Q) 0..1.... 0 1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 92. Lissorhpoterus simplex and Al/uaudomyia sp. presence and absence.

258 Q) () c Q) en ..Q co -Q) () c Q) en Q) a...!.....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 93. Tanysphyrus lemnae presence and absence.

Q) () c Q) en ..Q �

c() Q) en � a...

1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 94. Agabus sp., Ni/otanypus fimbriatus, Saetheria sp. 1, and Phaenopsectra sp. 2 presence and absence.

1 Q) () c Q) en ..Q � () c Q) en � a... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 95. Hygrotus sp., Micropsectra po/ita, and Helisoma anceps presence and absence.

259 Q) (.) c Q) (f) ..0 � (.) c Q) (f) Q) L... Cl..

. 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 96. Hygrotus!Hydroporus sp., Paratanytarsus (ct. laccophilus), Parachironomus vitosus gr., Parachironomus varus gr., Dicrotendipes tritomus, Pseudosmittia sp. 2, Culiseta inornata, Setacera sp., Corynoneura sp. 2, and Polypedilum trigonus presence and absence.

Q) (.) c Q) (f) ..0 � (.) c Q) (f) Q) L... Cl.. 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 97. 1/ybius sp. and Amnicola walkeri presence and absence.

Q) (.) c Q) (f) ..0 � (.) c Q) (f) � Cl..

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 98. Laccophilus sp. presence and absence. 260 Q) () c Q) en .0 � Q) () c Q) en � 0...

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 99. Neoporus sp. and Proc/adius freemani presence and absence.

Q) () c Q) en .0 ro -Q) () c Q) en � 0...

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 100. Haliplus sp. presence and absence.

Q) () c Q) en .0 � Q) () c Q) en � 0...

1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 101. Hydraena sp. and Paratendipes sp. 1 presence and absence.

261 Q) () c Q) CJ) ..c co (D () c Q) CJ) � a_

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 1 02. Anacaena sp. presence and absence.

Q) () c Q) CJ) ..c co (D () c Q) CJ) � a_

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 103. Berosus sp. and Glyptotendipes sp. 1 presence and absence.

Q) () c Q) CJ) ..c � Q) () c Q) CJ) Q) I... a_

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 104. Cymbiodyta sp., Guttipelopia sp., Stempellinella sp., Paratanytarsus inopertus gr. sp. 4, Zavre/iel/a sp., Zavrelia sp., Po/ypedi/um simulans, Paratendipes (?) sp. 2, Eristalis sp., Stratiomys sp., Dasyhelea sp., and Atrichopogon sp. presence and absence. 262 Q) () c Q) en ..c ro (D () c Q) en � D..

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 105. Enochrus sp. presence and absence.

Q) () c Q) en ..c � () c Q) en � D.. 0 1.5U 1.4 1.3 1.5D 1.1 1.2 2 Site

Figure 106. Helophorus sp. presence and absence.

1 Q) l () c Q) en ..c � () c Q) en � D..

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 107. Hydrobius sp., Polypedilum scalaenum gr. sp. 8, and Po/ypedilum fa/lax presence and absence.

263 a> (.) c a> en ..0 � (.) c a> en a> a...L...

1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 108. Hydrochus sp. presence and absence.

a> (.) c a> en ..0 ro (D (.) c a> en � a... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 109. Paracymus sp., Physel/a integra, and Pseudosuccinea columella presence and absence.

a> (.) c a> en ..0 ro (D (.) c a> en � a... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 110. Tropisternus sp. and Th ienemanniella simi/is presence and absence.

264 a> (.) c a> en ...0 co - a> (.) c a> en a> a..L..

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 111. C/inohelea!Heteromyia sp. and Gyrau/us parvus presence and absence.

a> (.) c a> en ...0 co -a> (.) c a> en a> a..L.. 0 t 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 112. Aedes cinereus presence and absence.

a> (.) c a> en ...0 � a> (.) c a> en a> a..L..

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 113. Aedes vexans, Larsia sp., and Chironomus sp. 1 presence and absence.

265 Q) () c Q) en .0 co 03 () c Q) en Q) a...s....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 114. Anopheles earlei presence and absence.

1 Q) () c Q) en .0 co -Q) () c Q) en Q) a...s.... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 115. Anopheles punctipennis and Cladotanytarsus sp. 1 presence and absence.

Q) () c Q) en .0 � Q) () c Q) en � a...

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 116. Culex territans presence and absence.

266 Q) (.) c Q) C/) .c co -Q) (.) c Q) C/) Q) D..!..... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 117. Dixella sp. presence and absence.

1 Q) (.) c Q) C/) .c co -Q) (.) c Q) C/) Q) D..!.....

0 - -r -1 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 118. Ephydra sp. presence and absence.

1 Q) (.) l c Q) C/) .c co -Q)

(.)c Q) C/) Q) D..!.....

0 I 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 119. Hydrellia sp. presence and absence.

267 Q) (.) c Q) (/) ...0 � (.) c Q) (/) � a_

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 120. Pericoma sp. presence and absence.

Q) (.) c Q) (/) ...0 � (.) c Q) (/) � a_

---, 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 121. Simulium sp. presence and absence.

Q) (.) c Q) (/) ...0 � (.) c Q) (/) � a_

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 122. Odontomyia!Hedriodiscus sp. and Fossaria modicella presence and absence.

268 1 Q) u c Q) en ..0 co -Q) u c Q) en Q) 0..."- 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 123. Oxycera sp., Polypedilum sp. 4, Polypedilum (nr. falciforme), Microtendipes sp. 3, Demeijeria sp., Cricotopuspol aris, and Prionocera sp. presence and absence.

1 Q) u c Q) en ..0 co -Q) u c Q) en Q) 0..."- 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 124. Chrysops sp. presence and absence.

Q) u c Q) en ..0 � u c Q) en � 0... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 125. Limnophila sp. presence and absence.

269 Q) (.) c Q) en .0 � Q) (.) c Q) en � 0...

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 126. Nematoda and Planorbella trivolvis presence and absence.

1 Q) (.) c Q) en .0 co -Q) (.) c Q) en Q) 0...!...... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 127. Musculium sp. presence and absence.

1 Q) 1 (.) c Q) en .0 � (.) c Q) en Q) 0...!......

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 128. Sphaerium sp. presence and absence.

270 Q) () c Q) C/) ..0 � () c Q) C/) � a_

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 129. Ferrissia tragi/is, Cryptochironomus eminentia, and Gammarus sp. presence and absence.

Q) () c Q) C/) ..0 � () c Q) C/) Q) 1... a_

------0 1 -- -- r � 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 130. Stagnico/a caperata and Chaetocladius sp. (weak, elongate AMS) presence and absence.

Q) () c Q) C/) ..0 � Q) () c Q) C/) � a_ 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 131. Stagnico/a elodes presence and absence.

271 Q) () c Q) en ..0 � Q) () c Q) en Q) 0.."-

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 132. Ap/exa elongata presence and absence.

Q) () c Q) en ..0 co 03 () c Q) en Q) 0.."- 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 133. Planorbula armigera, Th ienemanniella boltoni, Psectrocladius schlienzi type, and Cricotopus sylvestris presence and absence.

1 Q) () c Q) en ..0 � () c Q) en � 0..

- ---- 0 --- r ; -- I 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 134. Erpobdella punctata presence and absence.

272 Q) {.) c Q) (/) ..0 co CD {.) c Q) (/) Q) 0...!.....

1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 135. Glossiphonia complanata presence and absence.

Q) {.) c Q) (/) ..0 co CD {.) c Q) (/) Q) 0...!.....

0 - - r 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 136. Helobdella fusca presence and absence.

Q) {.) c Q) (/) ..0 � {.) c Q) (/) � 0...

1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 137. Placobdella papil/fera and Cricotopus tibialis presence and absence.

273 Q) (.) c Q) (/) .0 ro -Q) (.) c Q) (/) Q) ,_ Q_

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 138. Th eromyzon biannulatum, Phaenopsectra sp. 1, Cryptotendipes sp., Nanoc/adius incomptus, and Diplocladius sp. presence and absence.

1 Q) (.) c Q) (/) .0 ro 03 (.) c Q) (/) Q) ,_ Q_ 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 139. Acricotopus nitidellus presence and absence.

Q) (.) c Q) (/) .0 � (.) c Q) (/) Q) ,_ Q_ 0 -- -- ,- 1- 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 140. Corynoneura sp. 1 presence and absence.

274 Q) u c Q) (/) ..0 co 05 u c Q) (/) Q) !...... D...

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 141. Cricotopus bicinctus and Nanocladius distinctus presence and absence.

Q) u c Q) (/) ..0 co 05 u c Q) (/) � D... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 142. Cricotopus (lsocladius) sp. 1 and Doncricotopus bicaudatus presence and absence.

Q) u c Q) (/) ..0 co 05 u c Q) (/) Q) !...... D... 0 1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 143. Cricotopus sp. 5 presence and absence.

275 Q) (.) c Q) en ..0 ro (D (.) c Q) en Q) a..!.....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 144. Orthoc/adius oliveri and Polypedilum illinoense presence and absence.

Q) (.) c Q) en ..0 � (.) c Q) en Q) a..!.....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 145. Parametriocnemus sp. 1 presence and absence.

Q) (.) c Q) en ..0 � (.) c Q) en Q) a..!.....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 146. Paraphaenoc/adius exagitans and Tanytarsus sp. 1 ( cf. gregarius gr.) presence and absence.

276 1 Q) (.) c Q) (/) ..0 co -Q) () c Q) (/) Q) n.."- 0 1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 147. Thienemanniella sp. 1 presence and absence.

1 Q) (.) c Q) (/) ..0 co - Q) (.) c Q) (/) Q) n.."- 0 -1 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 148. Chironomus sp. 2 presence and absence.

Q) (.) c Q) (/) ..0 co - Q) (.) c Q) (/) Q) n..!o....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 149. Cladope/ma sp. 2 presence and absence.

277 Q) u c Q) en ..c co CD u c Q) en Q) a..!....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 150. Cryptochironomus sp. 1 (nr. conus) presence and absence.

1 Q) u c Q) en ..c � Q) u c Q) en Q) a..!....

0 -, 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 151. Cryptochironomus sp. 2 presence and absence.

Q) u c Q) en ..c co CD u c Q) en � a..

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 152. Endochironomus nigricans presence and absence.

278 Q) (.) c Q) CJ) ..c � (.) c Q) CJ) � a_

1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 153. Parachironomus fre quens gr. presence and absence.

1 Q) (.) c Q) CJ) ..c ro -Q) (.) c Q) CJ) Q) !..... a_ 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 154. Paralauterborniella nigrohalterale, Labrundinia pilosella, Tanytarsus confusus, and Polypedilum scalaenum gr. sp. 1 presence and absence.

Q) (.) c Q) CJ) ..c � (.) c Q) CJ) � a_ 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 155. Cladotanytarsus sp. 2 and Natarsia sp. presence and absence.

279 Q) u c Q) C/) .0 m (B u c Q) C/) Q) 0...L...

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 156. Paratanytarsus inopertus gr. sp. 1 and Tanytarsus sp. 10 presence and absence.

Q) u c Q) C/) .0 m -(]) u c (]) C/) (]) 0...L... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 157. Tanytarsus sepp presence and absence.

Q) u c Q) C/) .0 � u c Q) C/) Q) 0...L... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 158. Ta nytarsus sp. 12 presence and absence.

280 1 Q) 0 c Q) en ..0 co -Q) 0 c Q) en Q) 0....I.... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 159. Tanytarsus sp. 19 presence and absence.

Q) 0 c Q) en ..0 co -Q) 0 c Q) en Q) 0....I.... 0 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 160. Conchapelopia rurika presence and absence.

Q) 0 c Q) en ..0 � 0 c Q) en Q) 0....I....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 161. Paramerina sp. presence and absence.

281 Q) () c Q) CJ) ..0 � () c Q) CJ) Q) a..s.....

1.5U 1.4 1.3 1.50 1 1.1 1.2 2 Site

Figure 162. Procladius bel/us presence and absence.

Q) () c Q) CJ) ..0 � Q) () c Q) CJ) Q) a..s.....

0 �------I 1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 163. Procladius sublettei/denticulatus presence and absence.

Q) () c Q) CJ) ..0 � () c Q) CJ) Q) a..s.....

1.5U 1.4 1.3 1.50 1.1 1.2 2 Site

Figure 164. Tanypus sp. presence and absence.

282 Q) 0 c Q) CJ) .0 ro Q; 0 c Q) CJ) � a_

1.5U 1.4 New 1.3 1.50 1.1 1.2 2 Site

Figure 165. Endotribelos sp. larvae presence and absence.

Q) 0 c Q) CJ) .0 � 0 c Q) CJ) Q) !.... a_ 0 --, 1.5U 1.4 New 1.3 1.50 1.1 1.2 2 Site

Figure 166. Microchironomus sp. larvae presence and absence.

Q) 0 c Q) CJ) .0 ro -Q) 0 c Q) CJ) Q) !.... a_

1.5U 1.4 New 1.3 1.50 1.1 1.2 2 Site

Figure 167. Cricotopus trifascia and Clinotanypus sp. larvae presence and absence.

283 Q) (.) c Q) en ..0 � (.) c Q) en Q) 0..."-

1.5U 1.4 New 1.3 1.50 1 1.1 1.2 2 Site

Figure 168. Stackelbergina sp. larvae presence and absence.

Q) (.) c Q) en ..0 � Q) (.) c Q) en � 0...

1.5U 1.4 New 1.3 1.50 1.1 1.2 2 Site

Figure 169. Stilocladius sp. larvae presence and absence.

Q) (.) c Q) en ..0 co -Q) (.) c Q) en � 0...

1.5U 1.4 New 1.3 1.50 1.1 1.2 2 Site

Figure 170. Odontomesa sp. larvae presence and absence.

284 Q) 0 c Q) (J) ...0 � Q) 0 c Q) (J) Q) a..'-

1.5U 1.4 New 1.3 1.50 1 1.1 1.2 2 Site

Figure 171 . Monopelopia sp. larvae presence and absence.

Q) 0 c Q) (J) ...0 � Q) 0 c Q) (J) Q) a..'- 0 1.5U 1.4 New 1.3 1.50 1.1 1.2 2 Site

Figure 172. Pentaneura sp. larvae presence and absence.

285 Appendix C

A Comprehensive List of Macroinvertebrates in Hardwood Creek

Introduction

The fo llowing tables are a comprehensive list of the taxa collected in Hardwood Creek.

Hardwood Creek: Site H-1 .5U (2004) ON macroinvertebrates

TAXA 8A 88 8C 8k A 8k 8 8k C Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined (Collembola) 0 0 0 0 0 0 0 Insecta (class) EQhemeroQtera (order) Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 8aetidae Baetis sp. 0 0 0 0 0 0 0 Callibaetis sp. 0 0 0 0 0 0 0 Labiobaetis sp. 0 0 0 0 0 0 0 Undetermined (8aetidae) 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 0 0 0 0 0 0 Heptageniidae Heptagenia sp. 0 0 0 0 0 0 0 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 Odonata (order)

286 Calopterygidae Calopteryx sp. 0 0 0 0 0 0 0 Coenagrionidae Coenagrion!Enallagma sp. 0 0 0 0 0 0 0 lschnura sp. 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 Libellulidae Somatoch/ora sp. 0 0 0 0 0 0 0 Plecoptera (order) Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 Perlidae Per/esta sp. 0 0 0 0 0 0 0 Hemiptera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 1 1 Trichocorixa sp. 0 0 0 0 0 0 0 Undetermined (Corxidae immatures) 0 0 0 0 0 0 0 Gerridae Gerris sp. 0 0 0 0 0 0 0 Rheumatobates sp. 0 0 0 0 0 0 0 Hebridae Merra gata sp. 0 0 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 Mesoveliidae Mesovelia sp. 0 0 0 0 0 0 0 287 Nepidae Ranatra sp. 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 Pleidae Neoplea sp. 0 0 1 0 0 4 5 Veliidae Microve/ia sp. 0 0 0 0 0 0 0 Megaloptera (order) Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 Trichoptera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 Glossosomatidae Undetermined ( Glossosomatidae) 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 Hydroptilidae Agray/ea sp. 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 0 0 0 0 0 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 Nectopsyche sp. 0 0 0 0 0 0 0 Oecetis sp. 0 0 0 0 0 0 0 Tria enodes sp. 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 Lepidoptera (order) 288 Pyralidae Sync/ita sp. 0 0 0 0 0 0 0 Coleoptera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 Listronotas angustus* 0 0 0 0 0 0 0 Phyl/obius sp. 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 0 0 0 0 0 0 Tychius picirostrus 0 0 0 0 0 0 0 Undetermined (Curculionidae) 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 Hygrotus!Hydroporus sp. 0 0 0 0 2 0 2 1/ybius sp. 0 0 0 0 0 0 0 Laccophilus sp. 0 0 0 0 0 0 0 Liodessus sp. 0 0 0 0 0 0 0 Neopo_rus sp. 0 0 0 0 0 0 0 Elmidae Dubiraphia sp. 0 0 0 0 0 0 0 Optioservus sp. 0 0 0 0 0 0 0 Stene/mis sp. 0 0 0 0 0 0 0 Haliplidae Haliplus sp. 0 0 0 0 1 0 1 Peltodytes sp. 0 0 0 0 0 0 0 Hydraenidae Hydraena sp. 0 0 0 0 0 0 0 289 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 Berosus sp. 0 0 2 0 1 0 3 Cymbiodyta sp. 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 0 0 0 Helophorus sp. 0 0 0 0 0 0 0 Hydrobius sp. 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 0 0 0 Scirtidae Scirtes sp. 1 0 0 0 0 0 Staphylinidae Pontamalota sp. 0 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 Diptera (order) Undetermined (Diptera) 0 0 0 0 0 0 0 Ceratopogonidae Alluaudomyia sp. 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 Bezzia/Palpomyia sp. 0 0 2 0 0 0 2 Clinohe/ea!Heteromyia sp. 1 0 1 0 0 1 3 Cu/icoides sp. 0 0 0 0 0 0 0 Dasyhe/ea sp. 0 0 0 0 0 0 0 Ma/lochohelea sp. 0 0 0 0 0 0 0 Sphaeromias sp. 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 0 0 0 0 0 0 0 Chironomidae

290 Chironomini (tribe) Chironomus sp. 0 0 0 0 0 0 0 Cladopelma sp. 1 0 2 0 0 0 3 Cryptochironomus sp. 0 0 0 0 0 0 0 Cryptotendipes sp. 0 0 0 1 0 0 1 Dicrotendipes sp. 0 0 0 0 0 0 0 Einfeldia sp. 1 0 0 0 0 0 Endochironomus sp. 0 0 0 0 0 0 0 Endotribelos sp. 0 0 0 0 0 0 0 Glyptotendip es sp. 0 0 0 0 0 0 0 Microchironomus sp. 0 0 1 0 0 0 1 Parachironomus sp. 6 0 0 0 0 0 6 Paracladopelma sp. 0 0 0 0 0 0 0 Paralauterborniella sp. 0 0 0 0 0 0 0 Paratendipes sp. 0 0 0 0 0 0 0 Phaenopsectra sp. 0 0 0 0 0 0 0 Polyp edilum sp. 1 0 0 0 0 0 1 Pseudochironomus sp. 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 0 0 0 0 Stenochironomus sp. 0 0 0 0 0 0 0 Undetermined (Chironomini) 0 0 2 0 0 0 2 Zavreliella sp. 0 0 4 0 0 0 4 Orthocladiinae (subfamily) Acricotopus sp. 0 0 0 0 0 0 0 Corynoneura sp. 0 0 0 0 0 0 0 Cricotopus sp. 0 0 0 0 0 0 0 Cricotopus bicinctus gr. 0 0 0 0 0 0 0 Cricotopus trifa scia 0 0 0 0 0 0 0 Doncricotopus sp. 0 0 0 0 0 0 0 Nanocladius sp. 0 0 0 0 0 0 0 Parakiefferiella sp. 0 0 0 0 0 0 0

291 Parametriocnemus sp. 0 0 0 0 0 0 0 Psectro cladius sp. 0 0 0 0 0 0 0 Rheocricotopus sp. 0 0 0 0 0 0 0 Smittia sp. 0 0 0 0 0 0 0 Stackelbergina sp. 0 0 0 0 0 0 0 Stilocladius sp. 0 0 0 0 0 0 0 Th ienemanniella sp. 0 0 0 0 0 0 0 Tvetenia sp. 0 0 0 0 0 0 0 Undetermined (Orthocladiinae) 0 0 0 0 0 0 0 Prodiamesinae (subfamily) Odontomesa sp. 0 0 0 0 0 0 0 Tanypodinae (subfamily) Ablabesmyia sp. 1 0 1 0 0 0 2 Clinotanyp us sp. 0 0 0 0 0 0 0 Monopelopia sp. 0 0 1 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 Pentaneura sp. 0 0 0 0 0 0 0 Procladius sp. 6 0 11 1 2 1 21 Ta nypus sp. 1 0 0 0 0 0 1 Thienemannimyia gr. 0 0 0 0 0 0 0 Undetermined (Tanypodinae) 0 0 4 0 0 0 4 Tanytarsini (tribe) Cladotanytarsus sp. 0 0 0 0 0 0 0 Micropsectra sp. 0 0 0 0 0 0 0 Paratanytarsus sp. 0 0 0 0 1 0 1 Rheotanytarsus sp. 0 0 0 0 0 0 0 Stempellinella sp. 0 0 0 0 0 0 0 Tanytarsus sp. 0 0 1 0 0 2 3 Undetermined (Tanytarsini) 0 0 0 0 0 0 0 Culicidae Aedes sp. 0 0 0 0 0 0 0 292 Aedes cinereus 0 0 0 0 0 0 0 Aedes vexans 0 0 0 0 0 0 0 Anopheles sp. 0 0 0 0 0 0 0 Anopheles earlei 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 0 0 0 Culex sp. 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 Dixidae Dixella sp. 0 0 0 0 0 0 0 Dolichopod idae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 Empididae Chelife ra sp. 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 Ephydridae Ephydra sp. 0 0 0 0 0 0 0 Hydrellia sp. 0 0 0 0 0 0 0 Setacera sp. 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 0 0 0 0 0 Psychoda sp. 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 Te tanocera sp. 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 Simuliidae

293 Simulium sp. 0 0 0 0 0 0 0 Stratiomyidae Odontomyia sp. 0 0 0 0 0 0 0 Odontomyia/Hedriodiscus sp. 0 0 0 0 0 0 0 Oxycera sp. 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 0 0 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 Limnophila sp. 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Musculium sp. 0 0 0 0 0 0 0 Pisidium sp. 0 0 0 1 0 1 2 Sphaerium sp. 1 0 1 1 1 1 5 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 Gastropoda (class) Basommato[2hora (order} Undetermined (Gastropoda) 0 0 0 0 0 0 0 Ancylidae Ferrissia tra gi/is 0 0 0 0 0 0 0 Lymnaeidae 294 Fossaria modicel/a 0 0 0 0 1 0 1 Pseudosuccinea columella 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 0 0 Stagnicola elodes 0 0 0 0 0 0 0 Physidae Aplexa elongata 0 0 0 0 0 0 0 Physella gyrina 0 0 0 0 3 0 3 Physel/a integra 0 0 0 0 1 1 2 Planorbidae Gyraulus deflectus 0 0 0 0 0 0 0 Gyraulus parvus 0 0 0 0 0 0 0 Helisoma anceps 0 0 0 0 0 0 0 Planorbella trivolvis 0 1 0 0 1 0 2 Planorbula armigera 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 Heterostropha (order) Valvatidae Valvata tricarin ata 0 0 0 0 0 0 0 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 0 0 0 0 0 0 0 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdella punctata 1 0 1 2 1 2' 7 Nephelopsis obscura 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 Desserobdel/a michiganensis 0 0 0 0 0 0 0

295 Gloiobdella elongata 0 0 0 0 0 0 0 G/ossiphonia complanata 0 0 2 0 0 0 2 Helobdella fusca 1 0 0 0 0 2 3 Helobdella stagnalis 11 35 48 11 12 18 135 Placobdella papillifera 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 0 0 1 1 1 1 4 ARTHROPODA (phylum) CHELICERA TA (subphylum) Arachnida (class) Prostigmata (Suborder} Undetermined (Hydracarina) 0 1 6 0 1 2 10 CRUSTACEA (subphylum) Branchiopoda(class) Cladocera (suborder) Undetermined (Ciadocera) 0 0 0 0 0 0 0 Ostracoda (class) Undetermined (Ostracoda) 0 0 0 0 0 0 0 Copepoda (subclass) Undetermined (Copepoda) 0 0 0 0 0 0 0 Malacostraca (class) lsoQoda (order} Asellidae Caecidotea sp. 0 0 0 0 0 0 0 Amphipoda (order} Gammaridae Gammarus sp. 0 0 0 0 0 0 0 Hyalellidae Hyalle/a azteca 6 0 7 0 3 8 24 DecaQoda (order)

296 Cambaridae Orconectes virilis 0 0 0 0 0 0 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0

Table 1. Cumulative taxa list fo r ON samples at site 1.5U. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae and bottom (B) and bank (Bk) are the stratification units where samples were obtained. The letters A-C designate individual samples within stratification units.

Hardwood Creek: Site H-1 .4 (2004) ON macroinvertebrates

TAXA BA BB BC Bk A Bk B Bk C Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined (Collembola) 0 0 0 0 0 0 0 Insecta (class) E[2hemero[2tera (order} Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 0 0 0 0 0 0 Cal/ibaetis sp. 0 0 0 0 0 0 0 Labiobaetis sp. 0 0 0 0 0 0 0 Undetermined (Baetidae) 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 0 1 0 0 0 Heptageniidae Heptagenia sp. 0 0 0 0 0 0 0 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 Odonata (order} 297 Calopteryg idae Calopteryx sp. 0 0 0 0 0 0 0 Coenagrionidae Coenagrion/Enallagma sp. 0 0 0 0 0 0 0 lschnura sp. 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 Libellulidae Somatoch/ora sp. 0 0 0 0 0 0 0 Plecoptera (order) Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 Perlidae Perlesta sp. 0 0 0 0 0 0 0 Hemiptera (order) Belostomatidae Be/ostoma sp. 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 0 0 0 0 0 Undetermined (Corxidae imm�tures) 0 0 0 0 0 0 0 Gerridae Gerris sp. 0 0 0 0 0 0 0 Rheumatobates sp. 0 0 0 0 0 0 0 Hebridae Merragata sp. 0 0 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 Mesoveliidae Mesove/ia sp. 0 0 0 0 0 0 0 Nepidae Ranatra sp. 0 0 0 0 0 0 0 298 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 Pleidae Neoplea sp. 0 0 0 1 0 1 2 Veliidae Microve/ia sp. 0 0 0 0 0 0 0 Megaloptera (order) Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 Trichoptera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 Glossosomatidae Undetermined (Giossosomatidae) 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 Hydroptilidae Agraylea sp. 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 1 0 0 0 0 1 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 Nectopsyche sp. 0 0 0 0 0 0 0 Oecetis sp. 0 0 0 0 0 0 0 Tria enodes sp. 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 Lepidoptera (order) Pyralidae Sync/ita sp. 0 0 0 0 0 0 0 Coleoptera (order) Curculionidae 299 Lissorhpoterus simplex* 0 0 0 0 0 0 0 Listronotas angustus * 0 0 0 0 0 0 0 Phyllobius sp. 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus? 0 0 0 0 0 0 0 Tanysphyrus /emnae* 0 0 0 0 0 0 0 Tychius picirostrus 0 0 0 0 0 0 0 Undetermined (Curculionidae) 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 Hygrotus/Hydroporus sp. 0 0 0 0 0 0 0 1/ybius sp. 0 0 0 0 0 0 0 Laccophilus sp. 0 0 0 0 0 0 0 Liodessus sp. 0 0 0 0 0 0 0 Neoporus sp. 0 0 1 0 0 0 1 Elmidae Dubiraphia sp. 0 0 0 0 0 0 0 Optioservus sp. 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 Haliplidae Haliplus sp. 0 1 0 0 0 0 1 Peltodytes sp. 0 0 0 0 1 0 1 Hydraenidae Hydraena sp. 0 0 0 0 0 0 0 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 . Berosus sp. 0 0 0 0 0 0 0 Cymbiodyta sp. 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 0 0 0 Helophorus sp. 0 0 0 0 0 0 0 300 Hydrobius sp. 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 0 0 0 Scirtidae Scirtes sp. 0 0 0 0 0 0 0 Staphylinidae Pontamalota sp. 0 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 Diptera (order) Undetermined (Diptera) 0 0 0 0 0 0 0 Ceratopogonidae Alluaudomyia sp. 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 Bezzia/Palp omyia sp. 0 0 0 0 0 0 0 Clinohe/ea!Heteromyia sp. 1 1 0 0 2 0 4 Cu/icoides sp. 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 Mal/ochohelea sp. 0 0 0 0 0 0 0 Sphaeromias sp. 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 0 0 0 0 0 0 0 Chironomidae Chironomini (tribe) Chironomus sp. 0 0 1 0 0 0 1 Cladopelma sp. 0 0 0 0 0 0 0 Cryptochironomus sp. 0 0 0 0 2 1 3 Cryptotendipes sp. 0 0 0 0 0 0 0 Dicrotendipes sp. 0 0 0 0 0 0 0 Einfeldia sp. 0 0 0 0 0 0 0 Endochironomus sp. 0 0 0 1 0 0 1 301 Endotribelos sp. 0 0 0 0 0 0 0 G/yptotendip es sp. 0 0 0 0 0 0 0 Microchironomus sp. 0 0 0 0 0 0 0 Parachironomus sp. 0 0 0 0 0 0 0 Parac/adopelma sp. 0 0 0 0 0 0 0 Paralauterborniel/a sp. 0 0 0 0 0 0 0 Paratendipes sp. 3 0 5 0 2 0 10 Phaenopsectra sp. 0 0 0 0 0 0 0 Polypedilum sp. 0 1 0 0 0 0 1 Pseudochironomus sp. 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 0 0 0 0 Stenochironomus sp. 0 0 0 0 0 0 0 Undetermined (Chironomini) 0 0 0 0 0 0 0 Zavre lie/la sp. 0 0 0 0 0 0 0 Orthocladiinae (subfamily) Acricotopus sp. 0 0 0 0 0 0 0 Corynoneura sp. 0 0 0 0 0 0 0 Cricotopus sp. 0 1 0 1 0 0 2 Cricotopus bicinctus gr. 0 0 0 0 0 0 0 Cricotopus trifascia 0 0 0 0 0 0 0 Doncricotopus sp. 0 0 0 0 0 0 0 Nanocladius sp. 0 0 0 0 0 0 0 Parakiefferiella sp. 0 0 0 0 0 0 0 Parametriocnemus sp. 0 0 0 0 0 0 0 Psectrocladius sp. 0 0 0 0 0 0 0 Rheocricotopus sp. 0 0 0 0 0 0 0 Smittia sp. 0 0 0 0 0 0 0 Stackelbergina sp. 0 0 0 0 0 0 0 Stilocladius sp. 0 0 0 0 0 0 0 Th ienemanniella sp. 0 1 0 0 0 0 1 Tvetenia sp. 0 0 0 0 0 o· 0 Undetermined (Orthocladiinae) 0 0 0 0 0 0 0 Prodiamesinae (subfamily) 302 Odontomesa sp. 0 0 0 0 0 0 0 Tanypodinae (subfamily) Ablabesmyia sp. 0 0 0 0 0 0 0 Clinotanypus sp. 0 0 0 0 0 0 0 Monopelopia sp. 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 Pentaneura sp. 0 0 0 0 0 0 0 Procladius sp. 0 0 0 0 2 2 4 Tanypus sp. 0 0 0 0 0 0 0 Thienemannimyia gr. 0 1 0 0 0 0 1 Undetermined (Tanypodinae) 0 0 0 0 0 0 0 Tanytarsini (tribe) . Cladotanytarsus sp. 0 0 0 0 0 0 0 Micropsectra sp. 0 0 0 0 0 0 0 Paratanytarsus sp. 1 0 0 0 0 1 2 Rheotanytarsus sp. 0 0 0 0 0 0 0 Stempe/lin el/a sp. 0 0 0 0 0 0 0 Tanytarsus sp. 0 0 0 1 0 1 2 Undetermined (Tanytarsini) 0 0 0 0 0 0 0 Culicidae Aedes sp. 0 0 0 0 0 0 0 Aedes cinereus 0 0 0 0 0 0 0 Aedes vexans 0 0 0 0 0 0 0 Anopheles sp. 0 0 0 0 0 0 0 Anopheles earlei 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 0 0 0 Culex sp. 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 Dixidae Dixella sp. 0 0 0 0 0 0 0 Dolichopodidae 303 Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 Ephydridae Ephydra sp. 0 0 0 0 0 0 0 Hydrellia sp. 0 0 0 0 0 0 0 Setacera sp. 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 0 0 0 0 0 Psychoda sp. 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 Tetanocera sp. 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 Simuliidae Simulium sp. 0 0 0 0 0 0 0 Stratiomyidae Odontomyia sp. 0 0 0 0 0 0 0 Odontomyia!Hedriodiscus sp. 0 0 0 0 0 0 0 Oxycera sp. 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 0 0 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 Limnophila sp. 1 0 0 0 0 0 1 Prionocera sp. 0 0 0 0 0 0 0 304 Undetermined (Tipulidae) 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Muscu/ium sp. 1 2 0 0 0 0 3 Pisidium sp. 4 2 6 1 2 2 17 Sphaerium sp. 0 1 1 1 5 2 10 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 Gastropoda (class) BasommatoQhora (order) Undetermined (Gastropoda) 0 0 0 0 0 0 0 Ancylidae Ferrissia fra gilis 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicella 0 0 0 0 0 0 0 Pseudosuccinea columella 0 0 0 0 0 0 0 Stagnico/a caperata 0 0 0 0 0 0 0 Stagnico/a elodes 0 0 0 0 0 0 0 Physidae Aplexa elongata 0 0 0 0 0 0 0 Physella gyrina 1 0 1 0 0 2 4 Physel/a integra 0 0 0 0 0 0 0 Planorbidae Gyraulus deflectus 0 0 0 0 0 0 0 Gyraulus parvus 0 0 0 0 0 0 0 Helisoma anceps 0 0 0 0 0 0 0 Planorbella trivo/vis 0 0 0 0 0 0 0 Planorbula armigera 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 HeterostroQha (order) 305 Valvatidae Va lvata tricarinata 0 1 0 0 2 0 3 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 0 0 0 0 0 4 4 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdel/a punctata 0 0 0 0 0 1 1 Nephe/opsis obscura 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 Desserobdella michiganensis 1 0 0 0 0 0 1 G/oiobdella elongata 0 0 0 0 0 0 0 G/ossiphonia complanata 0 0 0 0 0 0 0 Helobdella fusca 0 0 0 0 0 0 0 Helobdella stagnalis 76 51 59 0 0 7 193 Placobdella papillifera 0 0 0 1 0 0 1 Th eromyzon biannulatum 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 1 0 1 1 5 ARTHROPODA (phylum) CHELICERATA (subphyl um) Arachnida (class) Prostigmata (Suborder} Undetermined (Hydracarina) 1 0 1 0 0 0 2 CRUSTACEA (subphylum) Branch iopoda(cla ss) Cladocera (suborder) Undetermined ( Cladocera) 0 0 0 0 0 0 0 Ostracoda (class) 306 Undetermined (Ostracoda) 0 0 0 0 0 0 0 Copepoda (subclass) Undetermined (Copepoda) 0 0 0 0 0 0 0 Malacostraca (class) lso�oda (order) Asellidae Caecidotea sp. 0 1 1 0 0 1 3 Am�hi�oda (order) Gammaridae Gammarus sp. 0 0 0 0 0 0 0 Hyalellidae Hyallela azteca 25 20 31 24 7 20 127 Deca�oda (order) Cambaridae Orconectes virilis 0 0 0 0 0 0 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0

Table 2. Cumulative taxa list for DN samples at site 1.4. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae and bottom (B) and bank (Bk) are the stratification units where samples were obtained. The letters A-C designate individual samples within stratification units.

Hardwood Creek: Site H-New (2004) ON macroinvertebrates

TAXA 8A 88 8C 8k A Bk 8 8k C WA W8 we Total ARTHROPODA (phylum) Entognatha (class) Collembola (order} Undetermined (Collembola) 0 0 0 0 0 0 0 0 0 0 Insecta (class) E�hemero�tera (order) 307 Undetermined ( E phemeroptera) 0 0 0 0 0 0 0 0 0 0 Baetidae . Baetis sp. 1 0 0 0 0 0 0 0 0 1 Cal/ibaetis sp. 0 0 0 0 0 0 0 0 0 0 Labiobaetis sp. 5 7 0 0 0 0 2 7 0 21 Undetermined (Baetidae) 0 0 0 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 5 1 0 0 1 1 4 1 1 3 Heptageniidae Heptagenia sp. 0 0 0 0 0 0 0 0 0 0 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 0 0 0 Odonata (order) Calopterygidae Ca/opteryx sp. 0 0 0 0 0 0 0 0 0 0 Coenagrion idae Coenagrion!Enallagma sp. 0 0 0 0 0 0 1 0 0 1 /schnura sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 0 0 0 Libellulidae Somatochlora sp. 0 0 0 0 0 0 0 0 0 0 PlecoQtera {order) Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 0 0 0 Perlidae Per/e sta sp. 0 0 0 0 0 0 0 0 0 0 HemiQtera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 0 0 0 308 Trichocorixa sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Corxidae immatures) 0 0 0 0 0 0 0 0 0 0 Gerridae Gerris sp. 0 0 0 0 0 0 0 0 0 0 Rheumatobates sp. 0 0 0 0 0 0 0 0 0 0 Hebridae Merragata sp. 0 0 0 0 0 0 0 0 0 0 Hyd rometridae Hydrometra sp. 0 0 0 0 0 0 0 0 0 0 Mesoveliidae Mesovelia sp. 0 0 0 0 0 0 0 0 0 0 Nepidae Ranatra sp. 0 0 0 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 0 0 0 Pleidae Neoplea sp. 0 0 0 0 0 0 1 4 0 5 Veliidae Micro velia sp. 0 0 0 0 0 0 0 0 0 0 MegaloQtera (order} Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 0 0 0 TrichoQtera (order} Undetermined {Trichoptera) 0 0 0 0 0 0 0 0 0 0 Glossosomatidae Undetermined (Giossosomatidae) 0 0 0 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 1 0 0 0 0 0 0 0 Hydroptilidae Agray/ea sp. 0 0 0 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 0 0 0 0 0 0 0 0 309 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 0 0 0 Nectopsyche sp. 0 0 0 0 0 0 0 0 0 0 Oecetis sp. 0 0 0 1 0 0 1 0 0 2 Tria enodes sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 0 0 0 Le�ido�tera (order} Pyralidae Sync/ita sp. 0 0 0 0 0 0 0 1 0 Coleo�tera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 0 0 0 Listronotas angustus* 0 0 0 0 0 0 0 0 0 0 Phyllobius sp. 0 0 0 0 0 0 0 0 0 0 Po/ydrusus sericeus 0 0 0 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 0 0 0 0 0 0 0 0 0 Tychius picirostrus 0 0 0 0 0 0 0 0 0 0 Undetermined (Curculionidae) 0 0 0 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 0 0 0 .. Hygrotus sp. 0 0 0 0 0 0 1 0 0 1 Hygrotus/Hydroporus sp. 0 0 0 0 0 0 0 0 0 0 1/ybius sp. 0 0 0 0 0 0 0 0 0 0 Laccophilus sp. 0 0 0 0 0 0 0 0 0 0 Liodessus sp. 0 0 0 0 0 0 0 0 0 0 Neoporus sp. 0 0 0 0 0 0 0 0 0 0 Elmidae Dubiraphia sp. 0 0 0 0 0 0 0 0 0 0 310 Optioservus sp. 0 0 0 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 0 0 0 Haliplidae Ha/iplus sp. 0 1 0 0 0 0 1 1 1 4 Peltodytes sp. 0 0 0 0 0 0 0 0 0 0 Hydraenidae Hydraena sp. 0 0 0 0 0 0 0 0 0 0 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 0 0 0 Berosus sp. 0 0 0 0 0 0 0 0 0 0 Cymbiodyta sp. 0 0 0 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 0 0 0 0 0 0 Helophorus sp. 0 0 0 0 0 0 0 0 0 0 Hydrobius sp. 0 0 0 0 0 0 0 0 0 0 Hydro chus sp. 0 0 0 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 0 0 0 0 0 0 Scirtidae Scirtes sp. 0 0 0 0 0 0 0 0 0 0 Staphylinidae Pontamalota sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 0 0 0 DiQtera (order} Undetermined (Diptera) 0 0 0 0 0 0 0 0 0 0 Ceratopogonidae Alluaudomyia sp. 0 0 0 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 0 0 0 Bezzia!Palpomyia sp. 0 1 0 3 0 0 0 1 0 5 C/inohelea/Heteromyia sp. 0 0 0 0 0 0 0 0 0 0 Culicoides sp. 0 0 0 0 0 0 0 0 0 0 311 Dasyhelea sp. 0 0 0 0 0 0 0 0 0 0 Mallochohelea sp. 1 0 0 1 0 0 0 0 3 5 Sphaeromias sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 0 0 0 0 0 0 0 0 0 0 Chironomidae Chironomini (tribe) Chironomus sp. 0 0 0 0 0 0 0 0 0 0 C/adopelma sp. 0 0 0 0 0 0 0 0 0 0 Cryptochironomus sp. 1 0 2 3 0 0 1 0 0 7 Cryptotendipes sp. 0 0 0 0 0 0 0 0 0 0 Dicrotendipes sp. 0 1 0 0 0 0 1 1 0 3 Einfeldia sp. 0 0 0 0 0 0 0 0 0 0 Endochironomus sp. 0 2 0 0 0 0 0 0 1 3 Endotribe/os sp. 0 1 0 0 0 0 0 0 0 1 G/yptotendipes sp. 0 0 0 0 0 0 0 0 0 0 Microchironomus sp. 0 0 0 1 0 0 0 0 0 1 Parachironomus sp. 2 4 1 0 0 0 1 5 0 13 Paracladopelma sp. 0 0 1 2 0 0 0 0 1 4 Paralauterborniella sp. 0 0 0 3 0 0 0 0 0 3 Para tendipes sp. 0 0 0 0 0 0 0 0 3 3 Phaenopsectra sp. 2 1 0 0 0 0 1 0 1 5 Polypedi/um sp. 0 0 0 1 0 0 3 3 0 7 Pseudochironomus sp. 0 0 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 0 0 0 0 0 0 0 Stenochironomus sp. 0 1 0 0 0 0 0 0 0 1 Undetermined (Chironomini) 0 0 0 0 0 0 0 0 0 0 Zavre /ie/la sp. 0 0 0 0 0 0 0 0 0 0 Orthocladiinae (subfamily) Acricotopus sp. 0 0 0 0 0 0 0 0 0 0 Corynoneura sp. 0 0 0 0 0 1 0 2 0 3 Cricotopus sp. 0 0 0 0 0 0 0 2 0 2 Cricotopus bicinctus gr. 0 2 0 0 0 0 1 1 0 4 Cricotopus trifascia 0 0 0 0 0 0 0 0 0 0 312 Doncricotopus sp. 0 0 0 0 0 0 2 0 1 3 Nanocladius sp. 0 17 0 0 0 0 3 16 0 36 Parakiefferiella sp. 0 0 0 0 0 0 0 0 0 0 Parametriocnemus sp. 0 0 0 0 0 0 0 0 0 0 Psectrocladius sp. 0 0 0 0 0 0 0 0 0 0 Rheocricotopus sp. 0 1 0 0 0 0 0 0 0 1 Smittia sp. 0 0 0 0 0 0 0 0 0 0 Stackelbergina sp. 0 0 0 0 0 0 0 1 0 1 Stilocladius sp. 0 0 0 0 0 0 0 0 0 0 Thienemanniella sp. 41 18 1 0 2 2 9 10 0 83 Tve tenia sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Orthocladiinae) 0 0 0 0 0 0 0 0 0 0 Prodiamesinae (subfamily) Odontomesa sp. 0 0 0 0 0 0 0 0 0 0 Tanypodinae (subfamily) Ablabesmyia sp. 0 1 0 1 0 2 3 3 1 11 Clinotanypus sp. 0 0 0 0 0 0 0 0 0 0 Monopelopia sp. 0 0 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 0 0 0 Pentaneura sp. 0 0 0 0 0 0 0 0 0 0 Pro cladius sp. 1 0 0 1 0 0 0 0 1 3 Tanypus sp. 0 0 0 0 0 0 0 0 0 0 Th ienemannimyia gr. 0 2 0 0 1 0 1 0 0 4 Undetermined (Tanypodinae) 0 0 0 0 0 0 0 0 0 0 Tanytarsini (tribe) Cladotanytarsus sp. 0 0 2 0 0 0 0 0 0 2 Micropsectra sp. 0 1 0 0 0 0 0 1 0 2 Paratanytarsus sp. 0 3 0 0 0 0 0 5 0 8 Rheotanytarsus sp. 0 2 0 0 0 0 2 2 0 6 Stempellinella sp. 1 0 0 0 0 0 0 0 0 1 Tanytarsus sp. 6 1 1 7 1 3 1 12 4 36 Undetermined (Tanytarsini) 0 0 0 0 0 0 0 0 0 0 Culicidae 313 Aedes sp. 0 0 0 0 0 0 0 0 0 0 Aedes cinereus 0 0 0 0 0 0 0 0 0 0 Aedes vexans 0 0 0 0 0 0 0 0 0 0 Anopheles sp. 0 0 0 0 0 0 0 0 0 0 Anopheles earlei 0 0 0 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 0 0 0 0 0 0 Culex sp. 0 0 0 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 0 0 0 Dixidae Dixella sp. 0 0 0 0 0 0 0 0 0 0 Dolichopodidae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 0 0 0 Ephydridae Ep hydra sp. 0 0 0 0 0 0 0 0 0 0 Hydrellia sp. 0 0 0 0 0 0 0 1 0 1 Setacera sp. 0 0 0 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 0 0 0 0 0 0 0 0 Psychoda sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 0 0 0 Te tanocera sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 0 0 0 Simuliidae Simulium sp. 148 329 6 0 0 2 21 71 0 577 314 Stratiomyidae Odontomyia sp. 0 0 0 0 0 0 0 0 0 0 Odontomyia!Hedriodiscus sp. 0 0 0 0 0 0 0 1 0 1 Oxycera sp. 0 0 0 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 1 0 0 0 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 0 0 0 Limnophila sp. 0 0 0 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 0 0 0 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order)

Pisidiidae · Musculium sp. 0 0 0 0 0 0 0 0 0 0 Pisidium sp. 3 4 22 4 4 2 1 6 2 48 Sphaerium sp. 0 0 0 0 0 0 0 0 1 1 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 0 0 0 Gastropoda (class) Basommatophora (order) Undetermined (Gastropoda) 0 0 0 0 0 0 0 0 0 0 Ancylidae Ferrissia tra gi/is 0 0 0 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicel/a 0 0 0 0 0 0 0 1 0 1 Pseudosuccinea columella 0 0 0 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 0 0 0 0 0 315 Stagnicola elodes 0 0 0 0 0 0 0 1 0 Physidae Aplexa elongata 0 0 0 0 0 0 0 0 0 0 Physella gyrina 2 2 0 0 0 0 7 3 0 14 Physella integra 0 0 0 0 0 0 0 0 0 0 Planorbidae Gyraulus deflectus 0 0 0 0 0 0 0 0 0 0 Gyra ulus parvus 0 0 0 0 0 0 1 1 0 2 Helisoma anceps 0 0 0 0 0 0 0 1 0 1 Planorbella trivo/vis 1 0 0 0 0 0 1 1 0 3 Planorbula armigera 0 0 0 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 0 0 0 Heterostropha (order) Valvatidae Va lvata tricarin ata 0 0 0 0 0 0 0 0 0 0 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 13 106 0 0 0 0 4 13 10 146 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdella punctata 0 12 0 0 0 1 1 3 1 18 Nephelopsis obscura 0 0 0 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 0 0 0 Desserobdella michiganensis 0 1 0 0 0 0 0 0 0 1 G/oiobdella elongata 0 0 0 0 0 0 0 0 0 0 Glossiphonia complanata 0 0 0 0 0 0 0 2 1 3 He/obdella fu sca 0 0 0 0 0 0 0 0 0 0 He/obdella stagnalis 2 3 0 1 0 0 0 3 5 14 Placobdel/a papillifera 1 0 0 0 0 1 0 1 0 3 316 Th eromyzon biannulatum 0 0 0 0 0 0 0 0 0 0

Oligochaeta (subclass) · Undetermined (Oiigochaeta) 1 1 0 1 0 1 1 1 1 7 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) Prostigmata (Suborder) Undetermined (Hydracarina) 0 0 0 0 0 0 0 3 0 3 CRUSTACEA (subphylum) Branch iopoda( class) Cladocera (suborder) Undetermined ( Cladocera) 0 0 0 0 0 0 0 0 0 0 Ostracoda (class) Undetermined (Ostracoda) 0 0 0 0 0 0 0 0 0 0 Copepoda (subclass) Undetermined (Copepoda) 0 0 0 0 0 0 0 0 0 0 Malacostraca (class) lsoQoda (order} Asellidae Caecidotea sp. 0 10 0 0 2 1 3 29 2 47 AmQhiQoda (order} Gammaridae Gammarus sp. 0 0 0 0 0 0 0 0 0 0 Hyalellidae Hyallela azteca 7 109 5 4 23 11 193 130 26 508 DecaQoda (order} Cambaridae Orconectes virilis 0 0 0 0 0 0 0 0 0 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0 0 0 0

317 Table 3. Cumulative taxa list fo r ON samples at site New. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae and bottom (B), bank (Bk), and wood (W) are the stratification units where samples were obtained. The letters A-C des_ignate individual samples within stratification units.

Hardwood Creek: Site H-1 .3 (2004) DN macroinvertebrates

TAXA BA BB BC Bk A Bk B Bk C WA WB we Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined (Collembola) 0 0 0 0 1 0 0 0 1 2 Insecta (class) EQhemeroQtera (order} Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 0 0 0 0 0 0 0 0 0 Cal/ibaetis sp. 0 0 0 0 0 0 0 0 0 0 Labiobaetis sp. 0 0 0 0 0 0 1 0 0 1 Undetermined (Baetidae) 0 0 0 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 0 0 0 1 0 2 0 0 3 Heptageniidae Heptagenia sp. 0 0 0 0 0 0 0 0 0 0 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 0 0 0 Odonata (order} Calopterygidae Calopteryx sp. 0 0 0 0 0 0 0 0 0 0 Coenagrionidae Coenagrion!Enallagma sp. 0 0 0 0 0 0 0 0 0 0 lschnura sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 0 0 0 318 Libellulidae Somatochlora sp. 0 0 0 1 0 0 0 0 0 Plecoptera (order) Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 0 0 0 Perlidae Per/esta sp. 0 0 0 0 0 0 0 0 0 0 Hemiptera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Corxidae immatures) 0 0 0 0 2 0 1 1 0 4 Gerridae Gerris sp. 0 0 0 0 0 0 0 0 0 0 Rheumatobates sp. 0 0 0 0 0 0 0 0 0 0 Hebridae Merragata sp. 0 0 0 0 0 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 0 0 0 Mesoveliidae Mesovelia sp. 0 0 0 0 0 0 0 0 0 0 Nepidae Ranatra sp. 0 0 0 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 1 0 0 Pleidae Neoplea sp. 0 0 0 0 2 0 11 1 5 19 Veliidae Microvelia sp. 0 0 0 0 0 0 0 0 0 0 319 Megaloptera (order) Corydalidae Chau/iodes sp. 0 0 0 0 0 0 0 0 0 0 Trichoptera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 0 0 0 Glossosomatidae Undetermined (Giossosomatidae) 0 0 0 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 0 0 0 Hydroptilidae Agray/ea sp. 0 0 0 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 1 0 0 0 1 0 0 2 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 1 0 0 1 Nectopsyche sp. 0 0 0 0 0 0 0 0 0 0 Oecetis sp. 0 0 0 0 0 0 0 0 0 0 Tria enodes sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 0 0 0 Lepidoptera (order) Pyralidae Sync/ita sp. 0 0 0 0 0 0 2 0 0 2 Coleoptera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 0 0 0 Listronotas angustus * 0 0 0 0 0 0 0 0 0 0 Phyl/obius sp. 0 0 0 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 0 0 1 0 0 0 0 1 Tanymecus nr. confertus ? 0 0 0 0 0 0 0 0 0 sp. I 0 Tanysphyrus lemnae* 0 0 0 0 0 0 0 0 0 0 320 Tychius picirostrus 0 0 0 0 0 0 0 0 0 0 Undetermined (Curculionidae) 0 0 0 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 3 0 3 Hygrotus!Hydroporus sp. 0 0 0 0 0 0 0 0 0 0 1/ybius sp. 0 0 0 0 0 0 0 0 1 1 Laccophilus sp. 0 0 0 0 0 0 0 0 0 0 Liodessus sp. 0 0 0 0 0 0 0 0 1 1 Neoporus sp. 0 0 0 0 0 0 0 0 0 0 Elmidae Dubiraphia sp. 0 0 0 0 0 0 0 0 0 0 Optioservus sp. 0 0 0 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 0 0 0 Haliplidae Haliplus sp. 3 0 0 0 0 0 11 1 2 17 Peltodytes sp. 1 0 0 0 0 0 8 3 4 16 Hydraenidae Hydra ena sp. 0 0 0 0 0 0 0 0 0 0 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 0 0 0 Berosus sp. 0 0 0 0 0 0 0 0 0 0 Cymbiodyta sp. 0 0 0 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 0 0 0 0 0 0 He/ophorus sp. 0 0 0 0 0 0 0 0 0 0 Hydrobius sp. 0 0 0 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 0 0 1 0 0 1 Ptiliidae 321 Undetermined (Ptiliidae) 0 0 0 0 0 0 0 0 0 0 Scirtidae Scirtes sp. 0 0 0 0 0 0 3 0 0 3 Staphylinidae Pontamalota sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 0 0 0 Diptera (order) Undetermined (Diptera) 0 0 0 0 0 0 0 0 0 0 Cerato pogon idae Al/uaudomyia sp. 0 0 0 0 1 0 0 0 0 1 Atrich opogon sp. 0 0 0 0 0 0 0 0 0 0 Bezzia/Palpomyia sp. 4 0 2 0 1 0 9 0 1 17 C/inohelea!He teromyia sp. 1 0 0 0 1 0 0 0 0 2 Culicoides sp. 0 0 0 0 0 0 0 0 0 0 Dasyhe/ea sp. 0 0 0 0 0 0 0 0 0 0 Mal/ochohelea sp. 0 0 0 0 0 1 0 0 0 1 Sphaeromias sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 0 0 0 0 0 0 4 0 0 4 Chironomidae Chironomini (tribe) Chironomus sp. 0 0 0 0 0 0 0 1 0 1 C/adopelma sp. 0 0 0 0 4 0 0 0 1 5 Cryptochironomus sp. 1 5 1 0 1 4 0 0 0 12 Cryptotendip es sp. 0 0 0 0 1 0 0 0 0 1 Dicrotendipes sp. 0 0 0 0 0 0 0 0 1 1 Einfeldia sp. 0 0 0 0 0 0 0 0 0 0 Endochironomus sp. 0 0 0 0 0 0 0 0 1 1 Endotrib elos sp. 0 0 0 0 0 0 0 0 0 0 G/yptotendip es sp. 0 0 0 0 0 0 0 0 0 0 Microchironomus sp. 0 0 0 0 1 0 0 0 0 1 Parachironomus sp. 0 0 0 0 0 0 0 0 0 0 Paracladopelma sp. 1 0 1 0 0 0 0 0 1 3 Paralauterborniella sp. 1 0 1 0 0 0 0 0 0 2

322 Paratendip es sp. 0 0 0 0 2 6 0 2 18 28 Phaenopsectra sp. 0 0 0 0 0 0 0 0 2 2 Polypedilum sp. 0 0 0 0 0 0 1 1 2 4 Pseudochironomus sp. 0 0 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 0 0 0 0 0 0 0 Stenochironomus sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Chironomini) 0 0 0 0 0 0 0 0 0 0 Zavre liella sp. 0 0 0 0 0 0 0 0 0 0 Orthocladiinae (subfamily) Acricotopus sp. 1 0 0 0 1 1 5 6 20 34 Corynoneura sp. 0 0 0 0 0 0 2 0 0 2 Cricotopus sp. 1 0 1 1 0 0 8 2 9 22 Cricotopus bicinctus gr. 3 0 1 6 2 0 10 1 18 41 Cricotopus trifascia 0 0 0 0 0 0 0 0 0 0 Doncricotopus sp. 0 0 2 1 0 0 2 0 2 7 Nanoc/adius sp. 0 0 0 3 0 0 0 0 2 5 Parakiefferiella sp. 0 0 0 0 0 0 0 0 0 0 Parametriocnemus sp. 0 0 0 0 0 0 0 0 0 0 Psectro cladius sp. 0 0 0 0 0 0 0 0 1 1 Rheocricotopus sp. 0 0 0 1 0 0 0 0 0 1 Smittia sp. 0 0 0 0 0 0 0 0 0 0 Stackelbergina sp. 0 0 0 0 0 0 0 0 0 0 Stilocladius sp. 0 0 0 0 0 0 0 0 0 0 Th ienemanniella sp. 0 0 0 3 0 0 0 0 0 3 Tve tenia sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Orthocladiinae) 0 0 0 0 0 1 0 0 0 1 Prodiamesinae (subfamily) Odontomesa sp. 0 0 0 0 0 0 0 0 0 0 Tanypodinae (subfamily) Ablabesmyia sp. 0 0 0 1 0 0 4 6 14 25 C/inotanypus sp. 0 0 0 0 0 0 0 0 0 0 Monopelopia sp. 0 0 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 0 0 0 323 Pentaneura sp. 0 0 0 0 0 0 0 0 0 0 Procladius sp. 0 0 0 0 0 0 0 1 10 11 Tanypus sp. 0 0 0 0 0 0 0 0 0 0 Th ienemannimyia gr. 0 0 0 0 0 0 0 0 0 0 Undetermined {Tanypodinae) 0 0 0 0 0 0 0 0 0 0 Tanytarsini (tribe) Cladotanytarsus sp. 0 0 1 0 0 0 0 0 0 1 Micropsectra sp. 0 0 0 3 0 1 1 0 0 5 Paratanytarsus sp. 0 0 0 2 0 0 3 0 2 7 Rheotanytarsus sp. 0 0 0 0 0 .o 0 0 0 0 Stempellin ella sp. 0 0 0 0 0 0 0 0 0 0 Tanytarsus sp. 5 0 3 0 2 1 2 1 22 36 Undetermined {Tanytarsini) 1 0 0 0 0 0 0 0 0 1 Culicidae Aedes sp. 0 0 0 0 0 0 0 0 0 0 Aedes cinereus 0 0 0 0 0 0 0 0 0 0 Aedes vexans 0 0 0 0 0 0 0 0 0 0 Anopheles sp. 0 0 0 0 0 0 0 0 0 0 Anopheles ear/ei 0 0 0 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 0 0 0 0 0 0 Culex sp. 0 0 0 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 0 0 0 Dixidae Dixella sp. 0 0 0 0 0 0 0 0 0 0 Dol ichopod idae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 0 0 0 Ephydridae Ephydra sp. 0 0 0 0 0 0 0 0 0 0 324 Hydrellia sp. 0 0 0 0 0 0 0 0 0 0 Setacera sp. 0 0 0 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 0 0 0 0 0 0 0 0 Psychoda sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 0 0 0 Te tanocera sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 0 0 0 Simuliidae Simulium sp. 1 0 0 1 0 0 1 0 0 3 Stratiomyidae Odontomyia sp. 0 0 0 0 0 0 0 0 0 0 Odontomyia!He driodiscus sp. 0 0 0 0 0 0 5 0 5 10 Oxycera sp. 0 0 0 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 0 0 0 0 0 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 0 0 0 Limnophila sp. 0 0 0 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 0 1 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) 325 Pisidiidae Musculium sp. 0 0 0 0 0 0 0 0 0 0 Pisidium sp. 2 2 1 0 13 4 0 1 33 56 Sphaerium sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 0 0 0 Gastropoda (class) BasommatoQhora (order) Undetermined (Gastropoda) 0 0 0 0 0 0 0 0 0 0 Ancylidae Ferrissia tra gi/is 0 0 0 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicel/a 0 0 0 0 0 0 5 0 1 6 Pseudosuccinea columella 0 0 0 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 0 0 0 0 0 Stagnicola elodes 0 0 0 0 0 0 0 0 0 0 Physidae Aplexa elongata 0 0 0 0 0 0 0 0 0 0 Physella gyrina 1 0 1 0 3 0 8 5 2 20 Physella integra 0 0 0 0 0 0 0 0 0 0 Planorbidae Gyra ulus deflectus 0 0 0 0 0 0 0 0 0 0 Gyra ulus parvus 0 0 0 0 0 0 8 1 5 14 Helisoma anceps 0 0 0 0 0 0 0 1 0 1 Planorbella trivolvis 0 0 0 0 0 0 5 3 0 8 Planorbu/a armigera 0 0 0 0 1 0 0 0 1 2 Promenetus exacuous 0 0 0 0 0 0 0 0 0 0 HeterostroQha (order) Valvatidae Valvata tricarin ata 0 0 0 0 0 1 0 0 0 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 3 0 0 0 0 1 10 1 10 25 ANNELIDA (phylum) 326 Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdella punctata 0 0 1 0 0 0 3 0 1 5

Nephelopsis obscura · 0 0 0 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae ' Albog/ossiphonia heteroclita 0 0 0 0 0 0 0 0 0 0 Desserobdel/a michiganensis 0 0 0 0 0 0 0 0 0 0 G/oiobdel/a elongata 0 0 0 0 0 0 0 0 0 0 G/ossiphonia complanata 1 0 0 0 1 0 1 0 3 6 Helobdella fu sca 0 0 0 0 1 0 0 0 0 1 Helobdella stagnalis 3 1 0 0 16 4 0 1 28 53 Placobdella papil/ifera 0 0 0 0 0 0 1 0 0 1 Th eromyzon biannulatum 0 0 0 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 1 1 1 1 1 1 1 9 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) Prostigmata (Suborder} Undetermined (Hydracarina) 0 0 0 0 0 0 0 0 CRUSTACEA (subphylum) Branch iopoda( class) Cladocera (suborder} Undetermined (Ciadocera) 0 0 0 0 0 0 0 0 0 0 Ostracoda (class) Undetermined (Ostracoda) 0 0 0 0 0 0 0 0 0 0 Copepoda (subclass) Undetermined (Copepoda) 0 0 0 0 0 0 0 0 0 0 Malacostraca (class) Isopod a (order} Asellidae 327 Caecidotea sp. 0 0 0 2 4 0 0 10 9 25 Amphipoda (order) Gammaridae Gammarus sp. 0 0 0 0 0 0 0 0 0 0 Hyalellidae Hyallela azteca 14 8 2 2 9 10 55 40 77 217 Decapoda (order) Cambaridae Orconectes virilis 0 0 0 1 0 0 0 0 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 1 0 0

Table 4. Cumulative taxa list for ON samples at site 1.3. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae and bottom (B), bank (Bk), and wood (W) are the stratification units where samples were obtained. The letters A-C designate individual samples within stratification units.

Hardwood Creek: Site H-1 .50 (2004) ON macroinvertebrates

TAXA BA BB BC Bk A Bk B Bk C WA WB we Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined (Collembola) 0 0 0 0 0 0 0 0 0 0 Insecta (class) Ephemeroptera (order} Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 0 0 0 0 0 0 0 0 0 Cal/ibaetis sp. 0 0 0 0 0 0 0 0 0 0 Labiobaetis sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Baetidae) 0 0 0 0 0 0 0 0 0 0 328 Caenidae Caenis sp. 0 1 1 0 0 0 1 a 0 3 Heptageniidae Heptagenia sp. 0 0 0 0 0 0 0 0 0 0 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 0 0 0 Odonata (order) Calopteryg idae Calopteryx sp. 0 0 0 0 0 0 0 0 0 0 Coenagrionidae Coenagrion!En al/agma sp. 0 0 0 0 0 0 0 0 0 0 lschnura sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 0 0 0 Libellulidae Somatoch/ora sp. 0 0 0 0 0 0 0 0 0 0 Plecoptera (order) Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 0 0 0 Perlidae Perle sta sp. 0 0 0 0 0 0 0 0 0 0 Hemiptera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Corxidae immatures) 0 0 0 0 0 1 0 0 0 1 Gerridae Gerris sp. 0 0 0 0 0 0 0 0 0 0 Rheumatobates sp. 0 0 0 0 0 0 0 0 0 0 Hebridae 329 Merragata sp. 0 0 0 0 0 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 0 0 0 Mesoveliidae Mesovelia sp. 0 0 0 0 0 0 0 0 0 0 Nepidae Ranatra sp. 0 0 0 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 0 0 0 Pleidae Neoplea sp. 0 0 1 0 1 0 0 0 0 2 Veliidae Micro velia sp. 0 0 0 0 0 0 0 0 0 0 Megaloptera (order) Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 0 0 0 Trichoptera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 0 0 0 Glossosomatidae Undetermined (Giossosomatidae) 0 0 0 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 0 0 0 Hydroptilidae Agray/ea sp. 0 0 0 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 1 0 0 0 0 0 0 1 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 0 0 0 Nectopsyche sp. 0 0 0 0 0 0 0 0 0 0 Oecetis sp. 0 0 0 0 0 0 0 0 0 0 Tria enodes sp. 1 0 0 0 0 0 0 0 0 1 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 0 0 0 330 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 0 0 0 Lepidoptera (order) Pyralidae Sync/ita sp. 0 0 1 0 0 0 0 0 0 Coleoptera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 0 0 0 Listronotas angustus* 0 0 0 0 0 0 0 0 0 0 Phyl/obius sp. 0 0 0 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 0 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 0 0 0 Tanysphyrus /emnae* 0 0 0 0 0 0 0 0 0 0 Ty chius picirostrus 0 0 0 0 0 0 0 0 0 0 Undetermined (Curculionidae) 0 0 0 0 0 0 0 1 0 1 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 0 0 0 Hygrotus/Hydroporus sp. 0 0 0 0 0 1 0 0 0 1 1/ybius sp. 0 0 0 0 0 0 0 0 0 0 Laccophilus sp. 0 0 0 0 0 0 0 0 0 0 Liodessus sp. 0 0 0 0 0 0 0 0 0. 0 Neoporus sp. 0 0 0 0 0 0 0 1 0 1 Elmidae Dubiraphia sp. 0 0 0 0 0 0 0 0 0 0 Optioservus sp. 0 0 0 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 0 0 0 Haliplidae Haliplus sp. 0 0 0 0 0 0 1 0 0 1 Peltodytes sp. 0 0 0 0 0 0 0 0 0 0 Hydraenidae 33 1 Hydraena sp. 0 0 0 0 0 0 0 0 0 0 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 0 0 0 Berosus sp. 0 2 5 0 0 4 0 1 0 12 Cymbiodyta sp. 0 0 0 0 0 0 o· 0 0 0 Enochrus sp. 0 0 0 0 0 0 0 0 0 0 Helophorus sp. 0 0 0 0 0 0 0 0 0 0 Hydrobius sp. 0 0 0 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 0 0 0 0 0 0 Scirtidae Scirtes sp. 0 0 0 0 0 0 0 0 0 0 Staphylinidae Pontamalota sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 0 0 0 Diptera (order) Undetermined (Diptera) 0 0 0 0 0 0 0 0 0 0 Ceratopogon idae Alluaudomyia sp. 0 0 0 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 0 0 0 Bezzia!Pa/pomyia sp. 1 0 0 0 0 0 0 0 0 1 Clinohelea/Heteromyia sp. 0 0 0 0 0 0 0 0 3 3 Culicoides sp. 0 0 0 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 0 0 0 Mallochohe/ea sp. 0 0 0 0 0 0 0 0 0 0 Sphaeromias sp. 0 0 0 0 0 0 0 0 0 0 Undetermined ( Ceratopogon idae) 0 0 0 0 0 0 0 0 0 0 Chironomidae Chironomini (tribe) 332 Chironomus sp. 0 0 0 2 0 1 0 0 0 3 Cladopelma sp. 0 0 0 0 1 0 0 0 0 1 Cryptochironomus sp. 0 2 0 0 0 0 0 2 1 5 Cryptotendipes sp. 0 0 0 0 0 0 0 0 0 0 Dicrotendip es sp. 0 0 1 0 0 0 0 2 0 3 Einfeldia sp. 0 0 0 0 0 0 0 0 0 0 Endochironomus sp. 1 0 1 0 0 0 0 2 0 4 Endotribelos sp. 0 0 0 0 0 0 0 0 0 0 G/yptotendip es sp. 0 0 0 0 0 0 0 0 0 0 Microchironomus sp. 0 0 0 0 0 0 0 0 0 0 Parachironomus sp. 6 3 1 2 0 1 5 2 1 21 Parac/adopelma sp. 0 0 0 0 0 0 0 0 0 0 Paralauterborniel/a sp. 0 0 0 0 0 0 0 0 0 0 Paratendipes sp. 0 0 5 8 0 5 1 1 0 20 Phaenopsectra sp. 0 0 0 2 0 0 0 0 0 2 Polyp edilum sp. 0 0 1 0 0 0 0 0 0 1 Pseudochironomus sp. 0 0 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 0 0 0 0 0 0 0 Stenochironomus sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Chironomini) 0 0 0 0 0 0 0 0 0 0 Zavreliella sp. 0 0 0 0 0 0 0 0 0 0 Orthocladiinae (subfamily) Acricotopus sp. 0 0 0 0 0 0 0 0 0 0 Corynoneura sp. 0 0 0 0 0 0 0 0 0 0 Cricotopus sp. 2 0 3 0 0 0 0 0 0 5 Cricotopus bicinctus gr. 0 1 1 0 0 0 2 0 1 5 Cricotopus trifascia 0 0 0 0 0 0 0 0 0 0 Doncricotopus sp. 0 0 0 0 0 0 0 0 0 0 Nanocladius sp. 0 0 2 0 0 0 0 0 0 2 Parakiefferiella sp. 0 0 0 0 0 0 0 0 0 0 Parametriocnemus sp. 0 0 0 0 0 0 0 0 0 0 Psectrocladius sp. 0 0 0 0 0 0 0 0 0 0 Rheocricotopus sp. 0 0 0 0 0 0 0 0 0 0 333 Smittia sp. 0 0 0 0 0 0 0 0 0 0 Stackelbergina sp. 0 0 0 0 0 0 0 0 0 0 Stilocladius sp. 0 0 0 0 0 0 0 0 0 0 Th ienemanniella sp. 0 0 1 0 0 0 1 0 3 5 Tve tenia sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Orthocladiinae) 0 0 0 0 0 0 0 0 0 0 Prodiamesinae (subfamily) Odontomesa sp. 0 0 0 0 0 0 0 0 0 0 Tanypodinae (subfamily) Ablabesmyia sp. 0 0 0 0 0 0 1 5 0 6 Clinotanypus sp. 0 0 0 0 0 0 0 0 0 0 Monopelopia sp. 0 0 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 0 0 0 Pentaneura sp. 1 0 0 0 0 0 0 1 0 2 Procladius sp. 0 0 0 2 0 1 0 0 0 3 Tanypus sp. 0 0 0 0 0 0 0 0 0 0 Thienemannimyia gr. 0 1 0 0 0 0 0 1 0 2 Undetermined (Tanypodinae) 0 0 0 0 0 0 0 0 0 0 Tanytarsini (tribe) Cladotanytarsus sp. 0 0 0 0 0 0 0 0 0 0 Micropsectra sp. 2 3 4 0 0 0 1 0 1 11 Paratanytarsus sp. 2 1 3 1 0 1 2 1 0 11 Rheotanytarsus sp. 0 0 0 0 0 0 0 0 0 0 Stempellinella sp. 0 0 0 0 0 0 0 0 0 0 Tanytarsus sp. 0 0 3 2 0 0 3 1 1 10 Undetermined (Tanytarsini) 0 0 0 0 0 0 0 0 0 0 Culicidae Aedes sp. 0 0 0 0 0 0 0 0 0 0 Aedes cinereus 0 0 0 0 0 0 0 0 0 0 Aedes vexans 0 0 0 0 0 0 0 0 0 0 Anopheles sp. 0 0 0 0 0 0 0 0 0 0 Anopheles earlei 0 0 0 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 0 0 0 0 0 0 334 Culex sp. 0 0 0 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 0 0 0 Dixidae Dixella sp. 0 0 0 0 0 0 0 0 0 0 Dolichopodidae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 0 0 0 Ephydridae Ephydra sp. 0 0 0 0 0 0 0 0 0 0 Hydrel/ia sp. 0 0 0 0 0 0 0 0 0 0 Setacera sp. 0 0 0 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 0 0 0 0 0 0 0 0 Psychoda sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 0 0 0 Te tanocera sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 0 0 0 Simuliidae Simulium sp. 0 0 0 0 0 0 0 0 0 0 Stratiomyidae Odontomyia sp. 0 1 0 0 0 0 0 0 0 1 Odontomyia!Hedriodiscus sp. 0 0 0 1 0 0 0 0 1 2 Oxycera sp. 0 0 0 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 0 0 0 Syrphidae 335 Eristalis sp. 0 0 0 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 0 0 0 0 0 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 0 0 0 Limnophila sp. 0 0 0 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 1 0 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Musculium sp. 2 0 0 1 0 0 0 0 0 3 Pisidium sp. 18 6 8 5 1 5 1 0 2 46 Sphaerium sp. 3 21 0 11 2 6 0 0 0 43 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 0 0 0 Gastropoda (class) Basommato(2hora (order} Undetermined (Gastropoda) 0 0 0 0 0 0 0 0 0 0 Ancylidae Ferrissia fra gilis 0 0 0 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicel/a 0 0 0 1 0 0 0 1 0 2 Pseudosuccinea columella 0 0 0 0 0 0 0 0 0 0 Stagnico/a caperata 0 0 0 0 0 0 0 0 0 0 Stagnicola elodes 0 0 0 0 0 0 0 0 0 0 Physidae Aplexa elongata 0 0 0 0 0 0 0 0 0 0 Physella gyrina 20 5 31 6 5 1 7 18 17 110 Physel/a integra 0 0 0 0 0 0 0 0 0 0 Planorbidae 336 Gyra u/us deflectus 0 0 3 0 0 0 0 2 0 5 Gyra u/us parvus 4 0 1 0 0 1 1 0 0 7 Helisoma anceps 0 0 0 0 0 0 0 0 0 0 Planorbella trivolvis 24 7 20 1 5 1 6 8 1 73 Planorbula armigera 2 0 2 0 0 0 0 1 0 5 Promenetus exacuous 10 0 7 0 0 1 0 8 0 26 Heterostropha (order) Valvatidae Valvata tricarinata 0 0 0 0 0 0 0 0 0 0 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 0 0 6 2 6 0 0 6 1 21 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdella punctata 5 2 0 7 0 2 1 4 3 24 Nephelopsis obscura 0 0 0 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 0 0 0 Desserobdella michiganensis 0 0 0 0 0 0 0 1 0 1 G/oiobdella elongata 0 0 0 0 0 0 0 0 0 0 G/ossiphonia complanata 1 2 2 0 0 1 0 1 0 7 Helobdella fusca 0 0 0 0 0 0 0 0 0 0 Helobdella stagnalis 44 69 40 12 2 45 7 44 2 265 Placobdel/a papillifera 0 0 0 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 0 Q. 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 1 1 1 1 1 1 1 9 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) 337 Prostigmata (Suborder) Undetermined (Hydracarina) 3 0 1 0 0 0 0 0 0 4 CRUSTACEA (subphylum) Branch iopoda(cla ss) Cladocera (suborder) Undetermined ( Cladocera) 0 0 0 0 0 0 0 0 0 0 Ostracoda (class) Undetermined (Ostracoda) 0 0 0 0 0 0 0 0 0 0 Copepoda (subclass) Undetermined (Copepoda) 0 0 0 0 0 0 0 0 0 0 Malacostraca (class) lso(2oda (order} Asellidae Caecidotea sp. 12 0 10 1 0 9 1 31 0 64 Am[2hi[2oda (order) Gammaridae Gammarus sp. 0 0 0 0 0 0 0 0 0 0 Hyalellidae Hyallela azteca 576 210 332 41 51 48 45 73 33 1409 Decar2oda (order} Cambaridae Orconectes virilis 0 0 0 0 0 0 0 0 0 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0 0 0 0

Table 5. Cumulative taxa list for ON samples at site 1.50. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae and bottom (B), bank (Bk), and wood (W) are the stratification units where samples were obtained. The letters A-C designate individual samples within stratification units.

338 Hardwood Creek: Site H-1 (2004) DN macroinvertebrates

TAXA BA BB BC BD BE Bk A Bk B Bk C WA WB we Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined ( Collembola) 0 0 1 0 0 0 0 0 0 0 0 Insecta (class) EQhemeroQtera (order) Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 0 0 1 3 0 0 0 0 0 0 4 Cal/ibaetis sp. 0 0 0 0 0 0 0 0 0 0 0 0 Labiobaetis sp. 0 1 0 1 2 0 0 0 6 1 15 26 Undetermined (Baetidae) 0 0 0 0 0 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 1 0 1 0 2 1 7 11 3 2 28 Heptageniidae Heptagenia sp. 0 0 0 0 0 0 0 0 3 0 0 3 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 0 0 0 0 0 Odonata (order) Calopteryg idae Calopteryx sp. 0 0 0 0 0 0 0 0 0 0 0 0 Coenagrionidae Coenagrion!Enallagma sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 lschnura sp. 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 0 0 0 0 0 Libellulidae Somatochlora sp. 0 0 0 0 0 0 0 0 0 0 0 0 PlecoQtera (order) Capniidae 339 Undetermined (Capniidae) 0 0 0 0 0 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 0 0 0 0 0 Perlidae Perlesta sp. 0 0 0 0 1 0 0 0 0 0 0 1 Hemiptera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 2 0 0 0 0 0 2 Trichocorixa sp. 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Corxidae immatures) 0 0 0 0 0 1 7 0 0 0 0 8 Gerridae Gerris sp. 0 0 0 0 0 1 0 0 0 0 0 1 Rheumatobates sp. 1 0 0 0 0 2 8 1 0 0 0 12 Hebridae Merra gata sp. 0 0 0 0 0 0 0 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 0 0 0 0 0 Mesoveliidae Mesovelia sp. 0 0 0 0 0 0 0 0 0 0 0 0 Nepidae Ranatra sp. 0 0 0 0 0 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 0 0 0 0 0 Pleidae Neoplea sp. 0 0 0 0 0 0 1 0 0 0 0 Veliidae Microvelia sp. 0 0 0 0 0 0 0 0 0 0 0 0 Meqaloptera (order) Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 0 0 0 0 0 Trichoptera (order) 340 Undetermined (Trichoptera) 0 0 0 0 0 0 0 0 0 0 0 0 Glossosomatidae Undetermined ( Glossosomatidae) 0 0 0 0 0 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 0 0 0 0 0 Hydroptilidae Agray/ea sp. 0 0 0 0 0 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 0 0 1 0 0 0 0 0 0 1 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Nectopsyche sp. 1 0 0 0 0 0 0 0 0 0 0 1 Oecetis sp. 9 0 4 0 0 1 0 0 0 0 1 15 Triaenodes sp. 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Lepidoptera (order) Pyralidae Syn c/ita sp. 0 0 0 0 0 0 0 0 0 0 0 0 Coleoptera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 0 0 0 0 0 Listronotas angustus* 0 0 0 0 0 0 0 0 0 0 0 0 Phyl/obius sp. 0 0 0 0 0 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 0 0 0 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 0 0 0 0 0 0 0 0 0 0 0 Tychius picirostrus 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Curculionidae) 0 0 0 0 0 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 0 0 0 0 0 341 Hydroporus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Hygrotus/Hydroporus sp. 0 0 0 0 0 0 0 0 0 0 0 0 1/ybius sp. 0 0 0 0 0 0 0 0 0 0 0 0 Laccophilus sp. 0 0 0 0 0 0 0 0 .0 0 0 0 Liodessus sp. 0 0 0 0 0 0 1 0 0 0 0 1 Neoporus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Elmidae Oubiraphia sp. 2 0 0 0 0 2 1 1 0 1 0 7 Optioservus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 0 0 0 0 0 Haliplidae Haliplus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Peltodytes sp. 0 0 0 0 0 0 0 0 1 0 0 1 Hydraenidae Hydraena sp. 0 0 0 0 0 0 0 0 0 0 0 0 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 0 0 0 0 0 Berosus sp. 0 0 0 0 0 0 0 0 0 0 1 1 Cymbiodyta sp. 0 0 0 0 0 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Helophorus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Hydrobius sp. 0 0 0 0 0 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 0 0 0 0 0 0 0 0 Scirtidae Scirtes sp. 0 0 0 0 0 0 0 0 0 0 0 0 Staphylinidae 342 Pontamalota sp. 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 0 0 0 0 0 Diptera (order) Undetermined (Diptera) 0 0 0 0 0 0 0 0 0 0 0 0 Ceratopogonidae Alluaudomyia sp. 0 0 0 0 0 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 0 0 0 0 0 Bezzia!Palpomyia sp. 0 0 1 0 0 0 0 1 2 0 2 6 Clinohelea!He teromyia sp. 0 0 0 0 0 0 1 0 0 0 0 1 Culicoides sp. 0 0 0 0 0 0 0 0 0 0 0 0 Oasyh elea sp. 0 0 0 0 0 0 0 0 0 0 0 0 Mallochohelea sp. 1 3 7 0 0 1 2 0 0 1 2 17 Sphaeromias sp. 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 0 0 0 0 0 0 0 0 0 0 0 0 Chironomidae Chironomini (tribe) Chironomus sp. 1 0 1 0 0 0 0 0 0 1 0 3 Cladopelma sp. 2 0 5 0 0 0 3 0 0 1 0 11 Cryptochironomus sp. 5 1 2 0 1 2 1 0 0 0 2 14 Cryptotendip es sp. 0 0 3 0 0 0 5 0 0 0 0 8 Dicrotendipes sp. 6 0 0 0 0 0 1 1 1 0 1 10 Einfeldia sp. 0 0 0 0 0 0 0 0 0 0 0 0 Endochironomus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Endotribelos sp. 0 0 0 0 0 0 0 0 0 0 0 0 G/yptotendip es sp. 0 0 0 0 0 0 0 0 0 1 0 1 Microchironomus sp. 0 0 11 0 0 0 0 0 0 0 0 11 Parachironomus sp. 0 0 1 0 0 0 0 0 5 2 1 9 Paracladopelma sp. 3 3 7 0 0 3 6 0 0 3 0 25 Paralauterborniel/a sp. 3 1 7 0 0 0 1 0 0 1 0 13 Paratendipes sp. 3 2 5 0 0 6 0 0 5 6 3 30 Phaenopsectra sp. 1 0 0 1 3 1 0 0 10 4 0 20 Polypedilum sp. 0 1 2 0 4 0 0 0 3' 0 1 11 Pseudochironomus sp. 0 0 0 0 0 0 0 1 0 0 0 1 343 Saetheria sp. 0 0 0 2 0 0 0 0 0 0 0 2 Stenochiro nomus sp. 0 0 0 0 0 0 0 0 0 1 1 2 Undetermined (Chironomini) 0 0 2 0 0 0 0 0 0 0 0 2 Za vre/iella sp. 0 0 0 0 0 0 0 0 0 0 0 0 Orthocladiinae (subfamily) Acricotopus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Corynoneura sp. 0 0 0 0 1 0 0 0 1 0 0 2 Cricotopus sp. 0 1 0 3 3 0 0 5 1 0 1 14 Cricotopus bicinctus gr. 2 1 0 0 12 0 0 2 2 0 7 26 Cricotopus trifascia 0 0 0 0 0 0 0 0 0 0 0 0 Doncricotopus sp. 13 27 3 0 1 1 0 4 0 1 0 50 Nanoc/adius sp. 5 24 0 0 1 0 0 0 15 5 6 56 Parakiefferiella sp. 0 2 0 1 0 0 0 0 0 0 0 3 Parametriocnemus sp. 0 0 0 0 0 0 0 0 1 1 0 2 Psectrocladius sp. 0 0 0 0 0 0 0 5 0 0 0 5 Rheocricotopus sp. 0 0 0 0 5 0 0 0 4 0 3 12 Smittia sp. 0 0 0 0 0 0 0 0 0 0 0 0 Stacke/bergina sp. 0 0 0 0 0 0 0 0 0 0 0 0 Stiloc/adius sp. 0 1 0 0 0 0 0 0 0 0 0 1 Thienemanniella sp. 0 1 0 8 36 0 0 1 32 2 14 94 Tvetenia sp. 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined ( Orthoclad i inae) 0 0 0 0 0 0 0 0 0 0 0 0 Prodiamesinae (subfamily) Odontomesa sp. 0 0 0 0 0 0 0 0 0 0 0 0 Tanypodinae (subfamily) Ablabesmyia sp. 0 1 2 0 0 0 0 1 13 5 1 23 C/inotanypus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Monopelopia sp. 0 0 0 0 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Pentaneura sp. 0 0 0 0 0 0 0 0 0 1 0 1 Pro cladius sp. 1 0 22 0 0 0 5 0 0 1 0 29 Tanypus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Th ienemannimyia gr. 0 0 0 0 5 0 0 0 0 2 0 7 344 Undetermined (Tanypodinae) 0 0 0 0 0 0 0 0 1 0 0 1 Tanytarsini (tribe) Cladotanytarsus sp. 2 2 0 4 1 0 0 0 0 0 0 9 Micropsectra sp. 0 2 0 0 0 0 0 0 1 1 1 5 Paratanytarsus sp. 0 0 0 0 0 0 0 1 2 2 2 7 Rheotanytarsus sp. 0 0 0 0 0 0 0 0 1 0 0 1 Stempellinella sp. 1 0 0 0 0 0 1 0 0 0 0 2. Tanytarsus sp. 65 27 66 2 4 5 8 0 9 4 3 193 Undetermined (Tanytarsini) 0 0 0 0 0 0 0 0 0 0 0 0 Culicidae Aedes sp. 0 0 0 0 0 0 0 0 0 0 0 0 Aedes cinereus 0 0 0 0 0 0 0 0 0 0 0 0 Aedes vexans 0 0 0 0 0 0 0 0 0 0 0 0 Anopheles sp. 0 0 0 0 0 0 0 0 0 0 0 0 Anopheles earlei 0 0 0 0 0 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 0 0 0 0 0 0 0 0 Culex sp. 0 0 0 0 0 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 0 0 0 0 0 Dixidae Dixella sp. 0 0 0 0 0 0 0 0 0 0 0 0 Dol ichopod idae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 0 0 0 0 0 Ephydridae Ephydra sp. 0 0 0 0 0 0 0 0 0 0 0 0 Hydrellia sp. 0 0 0 0 0 0 0 0 0 0 0 0 Setacera sp. 0 0 0 0 0 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 0 0 0 0 0 0 0 0 0 0 345 Psychoda sp. 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 0 0 0 0 0 Te tanocera sp. 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 0 0 0 0 0 Simuliidae Simulium sp. 4 0 0 42 297 0 0 3 84 4 49 483 Stratiomyidae Odontomyia sp. 0 0 0 0 0 0 0 0 0 0 0 0 Odontomyia!Hedrio discus sp. 0 0 0 0 0 0 0 0 0 0 0 0 Oxycera sp. 0 0 0 0 0 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 1 0 0 0 0 0 0 0 0 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 0 0 0 0 0. Limnophila sp. 0 0 0 0 0 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 0 0 0 0 0 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Musculium sp. 0 0 0 0 0 0 0 0 0 0 0 0 Pisidium sp. 2 36 35 2 1 3 23 10 0 3 20 135 Sphaerium sp. 0 7 15 0 0 2 2 3 3 1 3 36 346 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 0 0 0 0 0 Gastropoda (class) Basommatoghora (order} Undetermined (Gastropoda) 0 0 0 0 0 0 0 0 0 0 0 0 Ancylidae Ferrissia tra gi/is 0 0 0 0 0 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicel/a 0 0 0 0 0 0 0 0 0 0 0 0 Pseudosuccinea columella 0 0 0 0 0 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 0 0 0 0 0 0 0 Stagnicola elodes 0 0 0 0 0 0 0 0 0 0 0 0 Physidae Aplexa elongata 0 0 0 0 0 0 0 0 0 0 0 0 Physella gyrina 0 0 0 0 1 0 0 0 0 1 1 3 Physel/a integra 0 0 0 0 0 0 0 0 0 0 0 0 Planorbidae Gyra ulus deflectus 0 0 0 0 0 0 0 0 0 0 0 0 Gyra ulus parvus 0 0 0 0 0 0 0 0 0 0 1 1 Helisoma anceps 0 0 0 0 0 0 0 0 0 0 0 0 Planorbella trivolvis 0 0 0 0 0 0 0 0 0 0 0 0 Planorbula armigera 0 0 0 0 0 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 0 0 0 0 0 Heterostrogha (order} Valvatidae Va lvata tricarinata 0 0 0 0 0 0 0 0 0 0 0 0 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 0 0 0 0 1 0 0 0 1 0 0 2 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdella punctata 0 0 0 0 1 0 0 0 0 0 0 347 Nephelopsis obscura 0 0 0 0 0 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 0 0 0 0 0 Desserobdella michiganensis 0 0 0 0 0 0 0 0 0 0 0 0 Gloiobde/la elongata 0 0 0 0 0 0 0 0 0 0 0 0 Glossiphonia complanata 0 0 3 0 1 0 0 0 0 0 2 6 Helobde/la fusca 0 0 0 0 0 0 0 0 0 1 0 1 Helobdella stagnalis 1 0 22 0 0 0 3 0 0 2 4 32 Placobdella papillifera 0 0 0 0 0 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 1 1 1 1 1 1 1 1 1 11 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) Prostigmata (Suborder} Undetermined (Hydracarina) 0 0 0 0 0 0 0 0 1 1 0 2 CRUSTACEA (subphylum) Branch iopoda( class) Cladocera (suborder) Undetermined (Ciadocera) 0 0 0 0 0 0 0 0 0 0 0 0 Ostracoda (class) Undetermined (Ostracoda) 0 0 0 0 0 0 0 0 0 0 0 0 Copepoda (subclass) Undetermined (Copepoda) 0 0 0 0 0 0 0 0 0 0 0 0 Malacostraca (class) lso�oda (order} Asellidae Caecidotea sp. 0 0 7 0 2 0 0 3 25 10 13 60 Am�hipoda (order} Gammaridae Gammarus sp. 0 0 0 0 0 0 0 0 0 0 0 0

348 Hyalellidae Hyallela azteca 99 27 46 14 19 10 4 48 65 22 117 471 Decapoda (order) Cambaridae Orconectes virilis 0 0 0 0 0 0 0 0 0 1 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0 0 0 0 0 0

Table 6. Cumulative taxa list for DN samples at site 1. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae and bottom (B), bank (Bk), and wood (W) are the stratification units where samples were obtained. The letters A-E designate individual samples within stratification units.

Hardwood Creek: Site H-1 .1 (2004) DN macroinvertebrates

TAXA 8A 88 8C 8k A 8k 8 8k C WA W8 we Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined (Collembola) 1 0 0 2 0 0 7 4 0 14 Insecta (class) Ephemeroptera (order} Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 0 0 0 8aetidae Baetis sp. 0 0 1 0 0 0 0 0 0 1 Callibaetis sp. 0 0 0 0 0 0 0 0 0 0 Labiobaetis sp. 0 3 0 15 1 1 2 9 0 31 Undetermined (8aetidae) 0 0 0 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 1 0 2 1 0 0 8 6 18 Heptageniidae Heptagenia sp. 0 0 0 0 1 0 0 3 1 5 349 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 0 0 0 Odonata (order) Calopterygidae Calopteryx sp. 0 0 0 0 0 0 0 0 0 0 Coenagrionidae Coenagrion!Enallagma sp. 0 0 0 0 0 0 0 0 0 0 lschnura sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 0 0 0 Libellulidae Somatoch/ora sp. 0 0 0 0 0 0 0 0 0 0 Plecoptera (order) Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 0 0 0 Perlidae Perlesta sp. 0 0 0 0 0 0 0 0 Hemiptera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Corxidae immatures) 0 0 0 0 0 0 0 0 0 0 Gerridae Gerris sp. 0 0 0 0 0 0 0 0 0 0 Rheumatobates sp. 0 0 0 0 0 0 0 1 0 1 Hebridae Merragata sp. 0 0 0 0 0 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 0 0 0 Mesoveliidae 350 Mesovelia sp. 0 0 0 1 0 0 0 0 0 Nepidae Ranatra sp. 0 0 0 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 0 0 0 Pleidae Neop/ea sp. 0 0 0 1 1 0 0 0 0 2 Veliidae Microvelia sp. 0 0 0 0 0 0 0 0 0 0 Megaloptera (order) Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 0 0 0 Trichoptera (order} Undetermined (Trichoptera) 0 0 0 0 0 0 0 0 0 0 Glossosomatidae Undetermined (Giossosomatidae) 0 0 0 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 0 0 0 Hydroptilidae Agraylea sp. 0 0 0 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 0 0 0 0 0 0 0 0 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 0 0 0 Nectopsyche sp. 1 2 2 6 1 0 0 16 4 32 Oecetis sp. 1 0 0 0 0 0 0 1 2 4 Tria enodes sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 0 0 0 Lepidoptera (order} Pyralidae 351 Syn c/ita sp. 0 0 0 0 0 0 0 0 0 0 Coleoptera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 0 0 0 Listronotas angustus * 0 0 0 0 0 0 0 0 0 0 Phyl/obius sp. 0 0 0 0 0 0 0 0 0 0 Po/ydrusus sericeus 0 0 0 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 0 0 0 0 0 0 0 0 0 Ty chius picirostrus 0 0 0 0 0 0 0 0 0 0 Undetermined (Curculionidae) 0 0 0 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 0 0 0 Hygrotus!Hydroporus sp. 0 0 0 0 0 0 0 0 0 0 1/ybius sp. 0 0 0 0 0 0 0 0 0 0 Laccophilus sp. 0 0 0 0 0 0 0 0 0 0 Liodessus sp. 0 0 0 0 0 0 0 0 0 0 Neoporus sp. 0 0 0 0 1 0 0 0 0 1 Elmidae Dubiraphia sp. 11 0 0 11 34 3 2 4 1 66 Optioservus sp. 0 0 0 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 0 0 0 Haliplidae Halip/us sp. 1 0 0 0 0 0 0 0 0 1 Peltodytes sp. 0 0 0 0 0 0 0 0 0 0 Hydraenidae Hydraena sp. 0 0 0 0 0 0 0 0 0 0 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 0 0 0 Berosus sp. 0 0 0 1 0 0 0 0 0 1 352 Cymbiodyta sp. 0 0 0 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 1 0 0 0 0 1 Helophorus sp. 0 0 0 0 0 0 0 0 0 0 Hydrobius sp. 0 0 0 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 0 0 0 Sp erchopsis sp. 0 0 0 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptil i idae) 0 0 0 0 0 0 0 0 0 0 Scirtidae Scirtes sp. 0 0 0 0 0 0 0 0 0 0 Staphylinidae Pontamalota sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 0 0 0 Diptera (order) Undetermined (Diptera) 0 0 0 0 0 0 0 0 0 0 Ceratopogonidae Alluaudomyia sp. 0 0 0 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 0 0 0 Bezzia/Palpomyia sp. 0 0 0 0 0 0 0 0 0 0 Clinohelea/Heteromyia sp. 0 0 0 0 0 0 0 0 0 0 Culicoides sp. 0 0 0 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 0 0 0 Mal/ochohe/ea sp. 4 0 1 0 0 1 0 0 0 6 Sphaeromias sp. 0 0 0 0 0 0 0 0 0 0 Undetermined ( Ceratopogon idae) 0 0 0 0 0 0 0 0 0 0 Chironomidae Chironomini (tribe) Chironomus sp. 0 0 0 0 0 0 0 0 0 0 C/adopelma sp. 0 0 0 0 1 0 0 0 0 1 Cryptochironomus sp. 1 1 1 1 0 0 2 1 0 7 Cryptotendip es sp. 0 0 0 0 0 0 0 0 0 0 353 Dicrotendipes sp. 0 0 0 1 0 0 0 0 0 1 Einfe/dia sp. 0 0 0 0 0 0 0 0 0 0 Endochironomus sp. 0 0 0 0 0 1 0 0 1 2 Endotribelos sp. 0 0 0 0 0 0 0 1 0 1 Glyptotendipes sp. 0 0 0 0 0 0 0 0 1 1 Microchironomus sp. 0 0 0 0 0 0 0 0 0 0 Parachironomus sp. 0 0 0 0 0 1 0 0 0 1 Parac/adopelma sp. 0 0 0 0 0 0 0 0 0 0 Paralauterbornie/la sp. 1 0 0 0 0 0 0 0 0 1 Paratendipes sp. 0 0 0 1 0 0 0 1 1 3 Phaenopsectra sp. 2 0 0 0 1 0 0 1 11 15 Polypedilum sp. 0 0 3 0 0 0 0 0 0 3 Pseudochironomus sp. 0 0 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 0 0 0 0 0 0 0 Stenochironomus sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Chironomini) 0 0 0 0 0 0 0 0 0 0 Zavre/iella sp. 0 0 0 0 0 0 0 0 0 0 Orthocladiinae (subfamily) Acricotopus sp. 0 0 0 0 0 0 0 0 0 0 Corynoneura sp. 0 0 0 0 1 0 0 0 0 1 Cricotopus sp. 0 0 1 0 0 0 2 1 0 4 Cricotopus bicinctus gr. 0 2 4 0 2 2 0 5 0 15 Cricotopus trifascia 0 0 0 0 0 0 0 0 0 0 Doncricotopus sp. 0 0 5 0 0 2 1 0 0 8 Nanocladius sp. 0 1 1 0 2 0 1 1 3 9 Parakiefferiella sp. 0 0 0 0 0 0 1 0 0 1 Parametriocnemus sp. 0 0 0 0 0 0 0 0 0 0 Psectro cladius sp. 0 0 0 0 0 0 0 1 0 1 Rheocricotopus sp. 1 0 1 1 0 0 1 2 0 6 Smittia sp. 0 0 0 0 0 0 0 0 0 0 Stackelbergina sp. 0 0 0 0 0 0 0 0 0 0 Sti/ocladius sp. 0 0 0 0 0 0 0 0 0 0 Th ienemanniella sp. 0 9 13 2 2 1 0 4 0 31 354 Tve tenia sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Orthocladiinae) 0 0 0 0 0 0 0 0 0 0 Prodiamesinae (subfamily) Odontomesa sp. 0 1 0 0 0 0 0 0 0 Tanypodinae (subfamily) Ablabesmyia sp. 1 0 3 0 3 1 0 0 1 9 Clinotanypus sp. 0 0 0 0 0 0 0 0 0 0 Monopelopia sp. 0 0 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 0 0 0 Pentaneura sp. 0 0 0 0 0 0 0 0 0 0 Pro cladius sp. 0 0 0 1 1 0 0 0 0 2 Tanypus sp. 0 0 0 0 0 0 0 0 0 0 Th ienemannimyia gr. 0 0 0 0 1 0 0 0 0 1 Undetermined (Tanypod inae) 0 0 0 0 0 0 0 0 0 0 Tanytarsini (tribe) Cladotanytarsus sp. 1 0 3 0 0 0 0 0 0 4 Micropsectra sp. 0 0 0 0 0 0 0 1 0 1 Paratanytarsus sp. 0 0 0 1 0 0 0 0 0 1 Rheotanytarsus sp. 0 1 1 0 0 0 0 0 0 2 Stempellinella sp. 0 0 0 0 0 0 0 0 0 0 Ta nytarsus sp. 6 1 4 1 0 19 3 1 2 37 Undetermined (Tanytarsini) 0 0 0 0 0 0 0 0 0 0 Culicidae Aedes sp. 0 0 0 0 0 0 0 0 0 0 Aedes cinereus 0 0 0 0 0 0 0 0 0 0 Aedes vexans 0 0 0 0 0 0 0 0 0 0 Anopheles sp. 0 0 0 0 0 0 0 0 0 0 Anopheles earlei 0 0 0 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 0 0 0 0 0 0 Culex sp. 0 0 0 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 0 0 0 355 Dixidae Dixella sp. 0 0 0 0 0 0 0 0 0 0 Dolichopodidae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 0 0 0 Ephydridae Ephydra sp. 0 0 0 0 0 0 0 0 0 0 Hydrel/ia sp. 0 0 0 0 0 0 0 0 0 0 Setacera sp. 0 0 0 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 0 0 0 0 0 0 0 0 Psychoda sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 0 0 0 Te tanocera sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 0 0 0 Simuliidae Simulium sp. 0 5 3 0 0 0 0 10 0 18 Stratiomyidae Odontomyia sp. 0 0 0 0 0 0 0 0 0 0 Odontomyia!Hedriodiscus sp. 0 0 0 0 0 0 0 0 0 0 Oxycera sp. 0 0 0 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 1 0 0 0 0 0 0 0 0 Tipulidae 356 He/ius sp. 0 0 0 0 0 0 0 0 0 0 Limnophila sp. 0 0 0 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 0 0 0 Undetermined {Tipulidae) 0 0 0 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 0 0 1 0 0 0 1 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Musculium sp. 0 0 0 0 0 0 0 1 1 2 Pisidium sp. 13 0 5 3 17 3 0 4 3 48 Sphaerium sp. 0 0 0 0 0 0 0 0 0 0 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 0 0 0 Gastropoda (class) BasommatoQhora (order) Undetermined (Gastropoda) 0 0 0 0 0 0 0 0 0 0 Ancylidae Ferrissia fra gilis 0 0 0 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicella 0 0 0 0 0 0 0 0 0 0 Pseudosuccinea columella 0 0 0 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 0 0 0 1 1 Stagnicola elodes 0 0 0 0 0 0 0 0 0 0 Physidae Aplexa elongata 0 0 0 0 0 0 0 0 0 0 Physel/a gyrina 0 1 1 3 0 0 0 2 0 7 Physel/a integra 0 0 0 0 0 0 0 0 0 0 Planorbidae Gyrau/us deflectus 0 0 0 0 0 0 0 0 0 0 Gyraulus parvus 0 0 0 0 0 0 0 0 0 0 Helisoma anceps 0 0 0 0 0 0 0 0 0 0 Planorbella trivolvis 0 0 0 0 0 0 0 1 0 1 357

• Planorbula armigera 0 0 0 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 0 0 0 Heterostropha (order) Valvatidae Valvata tricarinata 0 0 0 0 0 0 0 0 0 0 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 0 0 0 0 0 0 0 0 0 0 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae ErpobdeJ/a punctata 0 0 0 0 2 0 0 0 1 3 Nephelopsis obscura 0 0 0 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 0 0 0 Desserobde/la michiganensis 0 0 0 0 0 0 0 0 0 0 Gloiobdella elongata 0 0 0 0 0 0 0 0 0 0 Glossiphonia complanata 0 0 0 0 0 0 0 1 0 1 Helobdel/a fu sca 0 0 0 0 0 0 0 0 0 0 Helobdel/a stagnalis 0 0 0 0 0 0 0 2 3 5 Placobdella papillifera 0 0 0 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 1 1 1 1 1 1 1 9 ARTHROPODA (phylum) CHELICERA TA (subphylum) Arachnida (class) Prostigmata (Suborder) Undetermined (Hydracarina) 1 0 0 3 0 0 1 0 0 5 CRUSTACEA (subphylum) Branchiopoda(class) 358 Cladocera (suborder} Undetermined ( Cladocera) 0 0 0 0 0 0 0 0 0 0 Ostracoda (class) Undetermined (Ostracoda) 0 0 0 0 0 0 0 0 0 0 Copepoda (subclass) Undetermined (Copepoda) 0 0 0 0 0 0 0 0 0 0 Malacostraca (class) Isopod a (order) Asellidae Caecidotea sp. 0 0 0 1 2 3 1 5 14 26 Amphipoda (order} Gammaridae Gammarus sp. 0 0 0 0 0 0 0 0 0 0 Hyalellidae Hyallela azteca 8 7 7 20 19 12 11 88 290 462 Decapod a (order) Cambaridae Orconectes virilis 0 0 0 1 1 0 0 0 0 2 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 1 0 0 0 0 0

Table 7. Cumulative taxa list for DN samples at site 1.1. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae and bottom (B), bank (Bk), and wood (W) are the stratification units where samples were obtained. The letters A-C designate individual samples within stratification units.

359 Hardwood Creek: Site H-1 .2 (2004) ON macroinvertebrates

TAXA BA BB BC BD BA BB BC WA WB we RA RB RC Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined (Collembola) 0 0 0 0 0 0 0 1 0 0 0 0 0 Insecta (class) EQhemeroQtera (order) Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 0 3 2 0 0 0 1 0 0 3 3 1 1 3 Callibaetis sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Labiobaetis sp. 0 0 0 0 1 0 24 0 0 0 0 0 0 25 Undetermined (Baetidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 0 0 0 0 0 1 0 0 0 0 0 0 Heptagen iidae Heptagenia sp. 0 0 7 0 0 0 2 7 0 2 0 0 1 19 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Odonata (order) Calopterygidae Ca/opteryx sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Coenagrion idae Coenagrion!Enal/agma sp. 1 0 0 0 0 0 0 0 0 0 0 0 0 1 /schnura sp. 0 0 0 0 0 0 0 0 0 o . 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Libellulidae Somatochlora sp. 0 0 0 0 0 0 1 0 0 0 0 0 0 PlecoQtera (order) Capniidae 360 Undetermined (Capniidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Perlidae Perlesta sp. 0 0 2 0 0 4 1 17 0 0 2 21 24 71 Hemiptera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Corxidae immatures) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Gerridae Gerris sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Rheumatobates sp. 0 0 0 0 2 0 0 0 0 0 0 0 0 2 Hebridae Merragata sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Hyd rometridae Hydrometra sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Mesoveliidae Mesovelia sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Nepidae Ranatra sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Pleidae Neop/ea sp. 1 0 0 0 0 0 0 0 0 0 0 0 0 Veliidae Microvelia sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Megaloptera (order) Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Trichoptera (order) 361 Undetermined (Trichoptera) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Glossosomatidae Undetermined (Giossosomatidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Hydroptilidae Agray/ea sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Nectopsyche sp. 0 0 0 0 0 7 12 2 0 1 1 0 0 23 Oecetis sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Tria enodes sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Lepidoptera (order) Pyralidae Syn c/ita sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Coleoptera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Listronotas angustus * 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Phyllobius sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus? 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Tychius picirostrus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Curculionidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 362 Hydroporus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 0 .o 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Hygrotus/Hydroporus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1/ybius sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Laccophi/us sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Liodessus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Neoporus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Elmidae Dubiraphia sp. 15 0 6 0 3 3 2 1 14 0 0 5 1 50 Optioservus sp. 0 0 0 0 0 0 0 0 0 0 0 1 0 1 Stenelmis sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Haliplidae Haliplus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Pe/todytes sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Hydraenidae Hydraena sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Berosus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Cymbiodyta sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Helophorus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Hydrobius sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 o· 0 0 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Scirtidae Scirtes sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Staphylinidae 363 Pontamalota sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Diptera (order) Undetermined (Diptera) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Ceratopogon idae Alluaudomyia sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Bezzia!Palpomyia sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 C/inohelea!Heteromyia sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Cu/icoides sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Mallochohelea sp. 3 0 0 0 1 4 0 0 1 0 0 1 0 10 Sphaeromias sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Chironomidae Chironomini (tribe) Chironomus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 C/adopelma sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Cryptochironomus sp. 0 0 0 1 2 1 0 1 0 0 0 1 0 6 Cryptotendipes sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Dicrotendipes sp. 0 0 0 0 0 1 0 0 0 0 0 0 0 1 Einfeldia sp. 0 0 0 0 0 0 0 0 1 0 0 0 0 1 Endochironomus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Endotribelos sp. 0 0 0 0 0 0 0 0 1 0 0 0 1 Glyptotendip es sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Microchironomus sp. 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Parachironomus sp. 0 0 0 0 0 1 0 0 1 0 0 0 0 2 Paracladopelma sp. 4 0 0 0 1 0 0 0 0 1 0 0 0 6 Paralauterborniel/a sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Paratendipes sp. 4 0 0 0 0 0 0 0 3 0 0 0 7 Phaenopsectra sp. 3 0 0 1 0 6 0 2 2 0 0 0 0 14 Polyp edilum sp. 0 2 0 1 0 1 2 3 0 0 1 3 2 15 Pseudochironomus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 364 Saetheria sp. 0 0 0 0 0 0 0 0 0 0 0 0 1 1 Stenochironomus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Chironomini) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Zavreliella sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Orthocladiinae (subfamily) Acricotopus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Corynoneura sp. 0 0 0 1 0 0 1 0 0 0 0 0 0 2 Cricotopus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Cricotopus bicinctus gr. 1 0 2 11 0 2 0 3 0 0 0 1 0 20 Cricotopus trifascia 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Doncricotopus sp. 0 0 0 1 2 0 0 0 0 0 0 0 0 3 Nanocladius sp. 1 0 1 0 3 0 2 3 1 0 0 0 0 11 Parakiefferiella sp. 0 0 0 0 0 1 0 0 0 0 0 0 0 1 Parametriocnemus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Psectrocladius sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Rheocricotopus sp. 0 0 1 1 0 0 0 25 0 0 0 5 2 34 Smittia sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Stacke/bergina sp. 0 0 0 0 0 0 0 0 0 o. 0 0 0 0 Stilocladius sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Th ienemanniella sp. 0 0 0 8 0 1 1 18 0 0 1 3 2 34 Tvetenia sp. 0 0 1 0 0 0 0 5 0 0 0 1 2 9 Undetermined (Orthocladiinae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Prodiamesinae (subfamily) Odontomesa sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Tanypodinae (subfamily) Ab/abesmyia sp. 15 0 0 0 2 3 1 0 3 0 0 0 0 24 Clinotanypus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Monopelopia sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 1 0 0 0 0 0 0 0 0 0 0 1 Pentaneura sp. 0 0 ·0 0 0 0 0 0 0 0 0 0 0 0 Pro cladius sp. 22 0 0 0 0 0 0 0 0 0 0 0 0 22 Tanypus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Thienemannimyia gr. 0 0 0 0 0 0 0 1 0 0 0 0 0 1 365 Undetermined (Tanypodinae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Tanytarsini (tribe) Cladotanytarsus sp. 0 0 7 19 0 0 0 28 0 0 4 51 32 141 Micropsectra sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Paratanytarsus sp. 1 0 0 0 0 0 0 0 0 0 0 0 0 1 Rheotanytarsus sp. 0 0 0 0 0 0 0 1 0 0 0 0 0 1 Stempellinel/a sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Tanytarsus sp. 9 0 6 8 1 0 0 16 0 0 0 2 8 50 Undetermined (Tanytarsini) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Culicidae Aedes sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Aedes cinereus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Aedes vexans 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Anopheles sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Anopheles earlei 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Culex sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Dixidae Dixella sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Dolichopodidae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Ephydridae Ep hydra sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Hydrel/ia sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Setacera sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 366 Psychoda sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Te tanocera sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Simuliidae Simulium sp. 0 0 0 0 0 0 0 0 0 0 4 2 0 6 Stratiomyidae Odontomyia sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Odontomyia!Hedrio discus sp. 0 0 0 0 0 0 1 0 0 0 0 0 0 1 Oxycera sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Limnophi/a sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0

• NEMATOD� (phylum) Undetermined (Nematoda) 0 0 0 0 0 0 0 0 0 0 1 0 0 1 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Musculium sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Pisidium sp. 0 0 0 0 6 20 0 0 15 0 0 1 0 42 Sphaerium sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 367 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Gastropoda (class) Basommatoghora (order} Undetermined (Gastropoda) 0 0 0 0 1 0 0 0 0 0 0 0 0 Ancylidae Ferrissia tra gi/is 0 0 5 0 0 1 0 0 0 0 0 2 0 8 Lymnaeidae Fossaria modicel/a 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Pseudosuccinea columella 0 0 0 0 0 1 0 0 0 0 0 0 0 1 Stagnicola caperata 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Stagnicola elodes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Physidae Aplexa elongata 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Physella gyrina 1 0 0 0 0 0 1 1 0 0 0 0 0 3 Physella integra 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Planorbidae Gyra ulus deflectus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Gyra ulus parvus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Helisoma anceps 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Planorbella trivolvis 0 0 0 0 0 0 0 0 0 0 0 0 1 1 Planorbula armigera 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Heterostrogha (order) Valvatidae Valvata tricarin ata 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Neotaenioglossa (order} Hydrobiidae Amnicola walkeri 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANNELIDA (phylum) Hirudinea (subclass) Arh�nchobdellida (order} Erpobdellidae Erpobdella punctata 0 0 0 0 0 0 0 0 0 0 0 0 0 0 368 Nephelopsis obscura 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Desserobdella michiganensis 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Gloiobdel/a elongata 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Glossiphonia complanata 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Helobdel/a fusca 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Helobdel/a stagnalis 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Placobdel/a papillifera 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 0 1 1 1 1 1 1 1 0 1 1 1 11 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) Prostigmata (Suborder} Undetermined (Hydracarina) 0 0 0 0 1 0 0 0 1 1 0 0 0 3 CRUSTACEA (subphylum) Branch iopoda(cla ss) Cladocera (suborder} Undetermined ( Cladocera) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Ostracoda (class) Undetermined (Ostracoda) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Copepoda (subclass) Undetermined (Copepoda) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Malacostraca (class) lso[2oda (order} Asellidae Caecidotea sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Am[2hi[2oda (order} Gammaridae Gammarus sp. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 369 Hyalellidae 4 Hya/lela azteca 24 0 1 0 12 18 2 16 36 6 2 1 122 Deca[2oda (order) Cambaridae Orconectes virilis 0 0 0 0 1 0 1 0 0 0 0 0 0 2 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 1 0 4 0 0 o . 0 0 0 5

Table 8. Cumulative taxa list for ON samples at site 1.2. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae and bottom (B), bank (Bk), wood (W), and riffle (R) are the stratification units where samples were obtained. The letters A-D designate individual samples within stratification units.

Hardwood Creek: Site H-2 (2004) DN macroinvertebrates

TAXA BA BB BC Bk A Bk B WA WB we Total ARTHROPODA (phylum) Entognatha (class) Collembola (order} Undetermined (Collembola) 0 0 0 0 0 1 0 1 2 Insecta (class) E[2hemero[2tera (order} Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 0 1 0 0 0 1 0 2 Callibaetis sp. 0 0 0 0 0 0 0 0 0 Labiobaetis sp. 0 0 1 1 1 18 12 26 59 Undetermined (Baetidae) 0 0 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 0 0 3 3 1 2 0 9 Heptageniidae Heptagenia sp. 0 0 2 0 2 26 29 3 62 370 Leptophlebiid�e Habrophlebiodes sp. 0 0 0 0 0 0 0 0 0 Odonata (order) Calopterygidae Ca/opteryx sp. 0 0 0 0 0 0 0 0 0 Coenagrion idae Coenagrion!Enal/agma sp. 0 0 0 0 0 0 0 0 0 /schnura sp. 0 0 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 0 0 Libellulidae Somatochlora sp. 0 0 0 1 0 0 0 0 Pleco(2tera (order} Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 0 0 Perlidae Per/esta sp. 0 0 2 0 0 5 6 0 13 Hemi(2tera (order} Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 0 0 0 0 0 0 0 Undetermined (Corxidae immatures) 0 0 0 0 0 0 0 0 0 Gerridae Gerris sp. 0 0 0 0 0 0 0 0 0 Rheumatobates sp. 0 0 0 0 0 0 0 0 0 Hebridae Merragata sp. 0 0 0 0 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 0 0 Mesoveliidae 371 · Mesovelia s p. 0 0 - 0 0 0 0 0 0 0 Nepidae Ranatra sp. 0 0 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 0 0 Pleidae Neoplea sp. 0 0 0 0 0 0 0 0 0 Veliidae Microve/ia sp. 0 0 0 0 0 0 0 0 0 Megalogtera (order} Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 0 0 Trichogtera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 0 0 Glossosomatidae Undetermined ( Glossosomatidae) 0 0 0 0 0 0 0 0 0 Hyd ropsych idae Cheumatopsyche sp. 0 0 0 0 0 0 0 0 0 Hydroptilidae Agraylea sp. 0 0 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 0 0 0 0 0 0 0 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 0 0 Nectopsyche sp. 0 3 0 1 4 5 14 1 28 Oecetis sp. 0 2 0 0 0 0 0 0 2 Tria enodes sp. 0 0 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 0 0 Legidogtera (order) Pyralidae 372 Sync/ita sp. 0 0 0 0 0 0 0 0 0 Coleo(2tera (order} Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 0 0 Listronotas angustus* 0 0 0 0 0 0 0 0 0 Phyllobius sp. 0 0 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 0 0 0 0 0 0 1 1 Tychius picirostrus 0 0 0 0 1 0 0 0 1 Undetermined (Curculionidae) 0 0 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 0 0 Hygrotus!Hydroporus sp. 0 0 0 0 0 0 0 0 0 1/ybius sp. 0 0 0 0 0 0 0 0 0 Laccophilus sp. 0 0 0 0 0 0 0 0 0 Liodessus sp. 0 0 0 0 1 0 0 0 1 Neoporus sp. 0 0 0 0 0 0 1 0 1 Elmidae Dubiraphia sp. 2 1 7 20 32 11 10 0 83 Optioservus sp. 0 0 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 0 0 Haliplidae Haliplus sp. 0 0 0 0 2 0 1 0 3 Peltodytes sp. 0 0 0 0 0 0 0 0 0 Hydraenida� Hydraena sp. 0 0 0 0 0 0 0 0 0 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 0 0 Berosus sp. 0 0 0 0 0 0 0 0 0 373 Cymbiodyta sp. 0 0 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 0 0 0 0 0 Helophorus sp. 0 0 0 0 0 0 0 0 0 Hydrobius sp. 0 0 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 0 0 0 0 0 Scirtidae Scirtes sp. 0 0 0 0 0 0 0 0 0 Staphylinidae Pontamalota sp. 0 0 0 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 0 0 DiQtera (order} Undetermined (Diptera) 0 0 0 0 0 0 0 0 0 Ceratopogonidae Alluaudomyia sp. 0 0 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 0 0 Bezzia/Palpomyia sp. 0 0 0 0 0 0 0 0 0 C/inohelea!He teromyia sp. 0 0 0 0 0 0 0 0 0 Culicoides sp. 0 0 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 0 0 Mal/ochohelea sp. 2 11 4 5 10 6 1 0 39 Sphaeromias sp. 0 0 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 0 0 0 0 0 0 0 1 1 Chironomidae Chironomini (tribe) Chironomus sp. 0 0 0 0 0 0 0 0 0 Cladopelma sp. 0 0 1 1 0 0 0 0 2 Cryptochironomus sp. 1 5 2 1 1 0 0 0 10 Cryptotendip es sp. 0 0 0 0 0 0 0 0 0 374 Dicrotendipes sp. 0 0 0 0 0 1 2 0 3 Einfe/dia sp. 0 0 0 0 0 0 0 0 0 En dochironomus sp. 0 0 0 0 1 1 0 0 2 Endotribe/os sp. 0 0 0 0 0 0 0 0 0 Glyptotendipes sp. 0 0 0 0 0 0 2 0 2 Microchironomus sp. 0 0 0 2 0 2 0 0 4 Parachironomus sp. 0 0 0 0 0 0 0 0 0 Parac/adopelma sp. 2 7 4 19 9 3 1 0 45 Paralauterborniel/a sp. 1 3 8 4 2 3 0 2 23 Paratendipes sp. 0 0 0 4 0 2 0 1 7 Phaenopsectra sp. 0 0 1 3 0 4 0 0 8 Po/ypedilum sp. 0 2 1 2 0 9 0 1 15 Pseudochironomus sp. 0 0 0 0 0 0 0 0 0 Saetheria sp. 1 1 2 0 0 0 0 0 4 Stenochironomus sp. 0 0 0 0 0 0 2 0 2 Undetermined (Chironomini) 0 2 2 1 0 4 0 0 9 Zavre liella sp. 0 0 0 0 0 0 0 0 0 Orthocladiinae (subfamily) Acricotopus sp. 0 0 0 0 0 0 0 0 ·o Corynoneura sp. 0 0 0 0 0 0 0 0 0 Cricotopus sp. 0 1 0 0 0 0 1 0 2 Cricotopus bicinctus gr. 0 1 5 0 0 5 9 1 21 Cricotopus trifa scia 1 0 0 0 0 1 0 0 2 Doncricotopus sp. 0 3 5 0 1 1 2 1 13 Nanocladius sp. 0 0 1 0 1 2 1 0 5 Parakiefferiel/a sp. 0 1 0 0 0 0 0 0 1 Parametriocnemus sp. 0 0 0 0 0 0 0 0 0 Psectro cladius sp. 0 0 0 0 0 0 0 0 0 Rheocricotopus sp. 0 1 1 0 0 14 9 0 25 Smittia sp. 0 0 0 0 1 0 0 0 1 Stacke/bergina sp. 0 0 0 0 0 0 0 0 0 Stilocladius sp. 0 0 0 0 0 0 0 0 0 Th ienemanniel/a sp. 0 1 1 0 0 6 10 2 20 375 Tve tenia sp. 0 0 0 0 0 1 0 0 1 Undetermined (Orthocladiinae) 0 0 0 0 0 0 0 0 0 Prodiamesinae (subfamily) Odontomesa sp. 0 1 0 0 0 0 0 0 Tanypodinae (subfamily) Ablabesmyia sp. 1 0 4 5 9 1 11 3 34 C/inotanypus sp. 0 0 0 1 0 0 0 0 1 Monopelopia sp. 0 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 0 0 Pentaneura sp. 0 0 0 0 0 0 0 0 0 Procladius sp. 0 0 1 53 9 2 3 0 68 Tanypus sp. 0 0 0 0 0 0 0 0· 0 Th ienemannimyia gr. 0 0 0 0 0 0 0 1 1 Undetermined (Tanypodinae) 0 0 1 0 0 0 0 0 1 Tanytarsini (tribe) C/adotanytarsus sp. 2 27 3 0 0 0 0 0 32 Micropsectra sp. 0 0 1 0 0 0 0 0 1 Paratanytarsus sp. 0 0 0 0 0 0 1 0 1 Rheotanytarsus sp. 0 0 0 0 0 0 1 0 1 Stempellinella sp. 0 0 0 0 0 0 0 0 0 Tanytarsus sp. 0 4 9 16 11 7 18 1 66 Undetermined (Tanytarsini) 0 0 0 0 0 0 1 0 1 Culicidae Aedes sp. 0 0 0 0 0 0 0 0 0 Aedes cinereus 0 0 0 0 0 0 0 0 0 Aedes vexans 0 0 0 0 0 0 0 0 0 Anopheles sp. 0 0 0 0 0 0 0 0 0 Anopheles earlei 0 0 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 0 0 0 0 0 Culex sp. 0 0 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 0 0 376 Dixidae Dixella sp. 0 0 0 0 0 0 0 0 0 Dolichopodidae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 -0 0 Empididae. Chelifera sp. 0 0 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 0 0 Ephydridae Ep hydra sp. 0 0 0 0 0 0 0 0 0 Hydrellia sp. 0 0 0 0 0 0 0 0 0 Setacera sp. 0 0 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 0 0 0 0 0 0 0 Psychoda sp. 0 0 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 0 0 Te tanocera sp. 0 0 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 0 0 Simuliidae Simulium sp. 0 0 0 0 0 2 0 0 2 Stratiomyidae Odontomyia sp. 0 0 0 0 0 0 0 0 0 . Odontomyia!Hedriodiscus sp. 0 0 0 0 0 0 0 1 1 Oxycera sp. 0 0 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 0 0 0 0 0 0 Tipulidae 377 He/ius sp. 0 o· 0 0 0 0 0 0 0 Limnophila sp. 0 0 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 0 0 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Musculium sp. 0 0 1 0 0 0 0 0 1 Pisidium sp. 0 0 4 1 5 0 1 0 11 Sphaerium sp. 0 0 0 0 0 0 0 0 0 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 0 0 Gastropoda (class) Basommatophora (order) Undetermined (Gastropoda) 0 0 0 0 0 0 0 0 0 Ancylidae Ferrissia fra gilis 0 0 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicella 0 0 0 0 0 1 0 0 1 Pseudosuccinea columella 0 0 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 1 0 0 1 Stagnicola elodes 1 0 0 0 0 0 0 0 1 Physidae Aplexa elongata 0 0 0 0 0 0 0 0 0 Physella gyrina 1 0 0 0 8 0 0 0 9 Physella integra 0 0 0 0 0 0 0 0 0 Planorbidae Gyra u/us deflectus 0 0 0 0 0 0 0 0 0 Gyraulus parvus 0 0 0 0 0 0 0 0 0 Helisoma anceps 0 0 0 0 0 0 0 0 0 P/anorbella trivolvis 0 0 0 0 0 0 0 0 0 378 P/anorbula armigera 0 0 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 0 0 Heterostropha (order) Valvatidae Va lvata tricarinata 0 0 0 0 0 0 0 0 0 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 0 0 0 0 0 0 0 0 0 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobde/la punctata 0 0 0 0 0 0 0 0 0 Nephelopsis obscura 0 0 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 0 0 Desserobdel/a michiganensis 0 0 0 0 0 0 0 0 0 G/oiobdella elongata 0 0 0 0 0 0 0 0 0 Glossiphonia complanata 0 0 0 0 0 0 0 0 0 Helobde/la fusca 0 0 0 0 0 0 0 0 0 He/obdella stagnalis 0 0 4 0 0 0 0 0 4 Placobdella papillifera 0 0 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 1 1 1 1 1 0 7 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) Prostigmata (Suborder) Undetermined (Hydracarina) 0 0 0 1 0 2 1 5 9 CRUSTACEA (subphylum) Branchiopoda(class) 379 Cladocera (suborder) Undetermined (Ciadocera) 0 0 0 0 0 0 0 0 0 Ostracoda (class) Undetermined (Ostracoda) 0 0 0 0 0 0 0 0 0 Copepoda (subclass) Undetermined (Copepoda) 0 0 0 0 0 0 0 0 0 Malacostraca (class) lsoQoda (order} Asellidae Caecidotea sp. 0 0 0 0 2 0 0 0 2 AmQhiQoda (order} Gammaridae Gammarus sp. 0 0 0 0 0 0 0 0 0 Hyalellidae Hyal/ela azteca 5 16 11 0 67 60 127 1 287 DecaQoda (order} Cambaridae Orconectes viri/is 0 0 0 0 0 1 2 0 3 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0 0 0

Table 9. Cumulative taxa list for ON samples at site 2. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae and bottom (B), bank (Bk), and wood (W) are the stratification units where samples were obtained. The letters A-C designate individual samples within stratification units.

380 Hardwood Creek: Site H-1 .5U (2004) Macroinvertebrates from SFPE samples

TAXA 4/28 5/26 6/23 7/28 9/1 10/6 11/13 Total ARTHROPODA (phylum) Entognatha {class) Collembola (order) Undetermined (Collembola) 1 1 1 1 0 1 1 6 Insecta {class) EQhemeroQtera (order} Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 0 0 0 0 0 0 0 Callibaetis sp. 0 1 1 0 1 0 1 4 Labiobaetis sp. 0 0 0 0 0 0 0 0 Undetermined (Baetidae) 0 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 0 0 1 1 0 0 2 Heptageniidae Heptagenia sp. 0 0 0 0 0 0 0 0 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 0 Odonata (order) Calopterygidae Calopteryx sp. 0 0 0 0 0 0 0 0 Coenagrionidae Coenagrion/Enallagma sp. 0 0 0 1 1 1 0 3 lschnura sp. 0 0 0 1 0 0 0 1 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 0

381 Libellulidae Somatochlora sp. 0 0 0 0 0 0 0 0 Plecoptera (order) Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 0 Perlidae Perlesta sp. 0 0 0 0 0 0 0 0 Hemiptera (order) Belostomatidae Be/ostoma sp. 0 0 0 0 1 0 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 0 1 1 0 0 2 Undetermined (Corxidae immatures) 0 0 1 1 1 0 0 3 Gerridae Gerris sp. 0 0 0 1 0 0 0 1 Rheumatobates sp. 0 0 0 0 0 0 0 0 Hebridae Merra gata sp. 0 0 1 0 0 0 0 1 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 0 Mesoveliidae Mesovelia sp. 0 1 1 1 1 0 0 4 Nepidae Ranatra sp. 0 0 0 1 0 0 0 Notonectidae Notonecta sp. 0 0 1 1 0 0 0 2

382 Pleidae Neoplea sp. 1 1 1 1 1 1 0 6 Veliidae Micro velia sp. 0 1 1 1 0 1 0 4 Megaloptera (order) Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 0 Trichoptera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 0 Glossosomatidae Undetermined (Giossosomatidae) 0 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 0 Hydroptilidae Agraylea sp. 0 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 1 0 0 0 0 Leptoceridae Leptocerus sp. 1 0 0 0 0 1 0 2 Nectopsyche sp. 0 0 0 1 1 0 0 2 Oecetis sp. 0 0 0 0 1 0 0 1 Tria enodes sp. 0 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 1 0 1 Lepidoptera (order) Pyralidae Syn c/ita sp. 1 1 0 0 1 1 0 4

383 Coleoptera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 0 Listronotas angustus* 0 0 0 0 0 0 0 0 Phyllobius sp. 0 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 1 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 0 1 0 0 0 0 1 Tychius picirostrus 1 1 0 0 0 0 0 2 Undetermined (Curculionidae) 0 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 0 Hygrotus/Hydroporus sp. 1 0 0 0 0 0 0 1 1/ybius sp. 0 0 0 0 0 0 0 0 Laccophi/us sp. 0 0 0 0 0 0 0 (j Liodessus sp. 0 0 0 1 1 0 0 2 Neoporus sp. 0 1 0 0 0 0 0 Elmidae Dubiraphia sp. 0 0 0 0 0 0 0 0 Optioservus sp. 0 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 0 Haliplidae Haliplus sp. 1 1 0 0 1 1 0 4 Peltodytes sp. 0 1 0 1 0 0 0 2 Hydraenidae Hydra ena sp. 0 0 0 0 0 0 0 0

384 Hydrophilidae Anacaena sp. 0 0 0 0 1 0 0 1 Berosus sp. 0 0 0 0 1 0 0 Cymbiodyta sp. 1 0 0 0 0 0 0 Enochrus sp. 0 1 0 0 0 0 0 Helophorus sp. 0 0 0 0 0 0 0 0 Hydrobius sp. 0 0 0 0 0 0 0 0 Hydrochus sp. 0 1 0 0 0 0 0 Paracymus sp. 1 0 0 0 0 0 0 1 Sperchopsis sp. 0 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 1 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 1 0 0 0 0 Scirtidae Scirtes sp. 1 1 0 0 0 1 0 3 Staphylinidae Pontamalota sp. 1 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 0 DiQtera (order) Undetermined (Diptera) 0 0 0 0 0 0 0 0 Ceratopogon idae A/luaudomyia sp. 0 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 1 0 Bezzia!Pa/pomyia sp. 1 1 0 1 1 1 0 5 C/inohelea!Heteromyia sp. 0 0 0 0 0 0 0 0 Culicoides sp. 0 0 0 0 0 0 0 0 Dasyhelea sp. 1 0 0 0 0 0 0 Mallochohelea sp. 0 0 0 0 0 0 0 0 Sphaeromias sp. 0 0 0 0 0 0 0 0

385 Undetermined (Ceratopogonidae) 1 1 0 1 1 0 0 4 Chironomidae Undetermined (Chironomidae) 1 1 1 1 1 1 1 7 Culicidae Aedes sp. 1 1 0 0 0 0 0 2 Aedes cinereus 0 0 0 0 0 0 0 0 Aedes vexans 1 0 0 0 0 0 0 Anopheles sp. 1 0 0 0 0 0 0 1 Anopheles earlei 0 0 0 0 0 0 0 0 Anopheles punctipennis 0 0 0 0 0 0 0 0 Culex sp. 0 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 0 Culiseta inornata 1 0 0 0 0 0 0 1 Undetermined (Culicidae) 0 0 0 0 0 0 0 0 Dixidae Dixella sp. 0 0 0 0 0 0 0 0 Dolichopodidae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 0 Ephydridae Ephydra sp. 0 0 0 0 0 0 Hydrellia sp. 1 0 0 0 0 0 1 2 Setacera sp. 0 1 0 1 0 0 0 2 Psychodidae Pericoma sp. 1 0 0 0 0 1 0 2 Psychoda sp. 0 0 0 0 0 0 0 0 Undetermined (Psychodidae) 1 0 0 0 0 0 0

386 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 1 1 0 0 0 0 0 2 Te tanocera sp. 1 0 0 1 1 1 0 4 Undetermined (Sciomyzidae) 0 0 0 0 0 1 0 1 Simuliidae Simulium sp. 0 0 0 0 0 0 0 0 Stratiomyidae Odontomyia sp. 1 1 1 0 0 0 0 3 Odontomyia/Hedriodiscus sp. 1 1 1 1 0 0 0 4 Oxycera sp. 0 0 0 0 0 0 0 0 Stratiomys sp. 1 0 0 0 0 0 0 Syrphidae Eristalis sp. 1 0 0 0 0 0 0 Tabanidae Chrysops sp. 1 0 0 0 0 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 0 Limnophi/a sp. 0 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 1 1 0 0 1 0 0 3 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Musculium sp. 0 0 0 0 0 0 0 0

387 Pisidium sp. 0 0 0 0 0 0 0 0 Sphaerium sp. 1 0 0 0 0 0 0 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 0 Gastropoda (class) BasommatoQhora (order) Undetermined (Gastropoda) 0 0 0 0 0 0 0 0 Ancylidae Ferrissia fra gi/is 0 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicel/a 1 1 1 1 1 1 1 7 Pseudosuccinea columella 1 0 0 0 0 0 0 1 Stagnicola caperata 0 0 0 0 0 0 0 0 Stagnicola elodes 1 0 0 0 0 0 0 1 Physidae Ap/exa elongata 1 1 0 0 0 0 0 2 Physella gyrina 0 1 1 1 1 1 0 5 Physella integra 0 0 0 0 0 0 0 0 Planorbidae Gyraulus deflectus 0 0 0 0 0 0 0 0 Gyra ulus parvus 1 1 1 1 1 1 0 6 Helisoma anceps 0 0 0 0 0 0 0 0 Planorbella trivolvis 0 1 1 1 1 0 0 4 Planorbu/a armigera 0 1 0 0 0 0 0 Promenetus exacuous 1 0 0 0 1 1 0 3 HeterostroQha (order) Valvatidae Va /vata tricarin ata 0 0 0 0 1 0 0 Neotaenioglossa (order) Hydrobiidae

388 Amnicola walkeri 0 0 0 0 0 0 0 0 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdella punctata 0 0 0 0 0 0 0 0 Nephelopsis obscura 0 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 0 Desserobdella michiganensis 0 0 0 0 0 0 0 0 Gloiobdella elongata 0 0 0 0 0 0 0 0 Glossiphonia complanata 0 0 0 0 1 0 0 1 Helobde/la fusca 0 0 0 0 0 0 0 0 Helobdella stagnalis 0 0 0 1 1 1 0 3 Placobdella papillifera 0 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 1 1 1 1 1 7 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) Prostigmata (Suborder) Undetermined (Hydracarina) 1 1 1 1 1 1 1 7 CRUSTACEA (subphylum) Branch iopoda(class) Cladocera (suborder) Undetermined ( Cladocera) 1 1 1 1 1 1 1 7 Ostracoda (class)

389 Undetermined (Ostracoda) 1 0 6 Copepoda (subclass) Undetermined (Copepoda) 1 7 Malacostraca (class) lsopoda (order) Asellidae Caecidotea sp. 0 0 0 0 0 0 1 Amphipoda (order) Gammaridae Gammarus sp. 0 0 0 0 0 0 0 0 Hyalellidae Hyallela azteca 0 0 5 Decapod a (order) Cambaridae Orconectes virilis 0 0 0 0 0 0 0 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0 0

Table 10. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1.5U. An asterisk (*) indicates aquatic or semi­ aquatic Curculionidae. Only the presence (1) or absence (0) of each taxon is recorded.

Hardwood Creek: Site H-1 .4 (2004) Macroinvertebrates from SFPE samples

TAXA 5/26 6/23 7/28 9/1 10/6 11/13 Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined (Collembola) 1 1 1 0 0 4 Insecta (class) 390 EQhemeroQtera (order} Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 0 1 1 1 1 4 Cal/ibaetis sp. 0 0 0 1 0 0 1 Labiobaetis sp. 0 0 0 0 0 0 0 Undetermined (Baetidae) 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 0 0 1 1 1 3 Heptageniidae Heptagenia sp. 0 0 0 0 0 0 0 Leptophlebiidae Habroph/ebiodes sp. 0 0 0 0 0 0 0 Odonata (order} Calopteryg idae Calopteryx sp. 0 0 0 0 0 0 0 Coenagrionidae Coenagrion/Enal/agma sp. 0 0 1 0 1 0 2 lschnura sp. 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 Libellulidae Somatochlora sp. 0 0 0 0 0 0 0 PlecoQtera (order} Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 Perlidae Perlesta sp. 0 0 0 0 0 0 0 HemiQtera (order} Belostomatidae Belostoma sp. 0 0 0 1 0 0 Corixidae 391 Sigara sp. 0 0 0 0 0 0 0 Trichocorixa sp. 0 1 0 1 1 1 4 Undetermined (Corxidae immatures) 1 0 1 1 1 0 4 Gerridae Gerris sp. 0 0 0 0 0 0 0 Rheumatobates sp. 0 0 0 0 0 0 0 Hebridae Merragata sp. 0 0 0 0 1 0 1 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 Mesoveliidae Mesove/ia sp. 0 1 0 1 1 0 3 Nepidae Ranatra sp. 0 0 0 0 0 Notonectidae Notonecta sp. 0 1 1 0 0 0 2 Pleidae Neoplea sp. 1 1 1 1 1 0 5 Veliidae Microvelia sp. 0 1 1 1 1 1 5 Meqaloptera (order) Corydalidae Chau/iodes sp. 0 0 0 0 0 0 0 Trichoptera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 Glossosomatidae Undetermined ( Glossosomatidae) 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 Hydroptilidae Agraylea sp. 0 0 0 1 1 0 2 Orthotrichia sp. 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 1 1 0 2 392 Undetermined (Hydroptilidae) 0 1 0 1 1 0 3 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 Nectopsyche sp. 0 0 0 0 0 0 0 Oecetis sp. 0 0 0 1 0 0 1 Triaenodes sp. 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 1 0 0 Lepidoptera (order) Pyralidae Syn c/ita sp. 0 0 1 1 1 0 3 Coleoptera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 Listronotas angustus* 0 0 0 0 0 0 0 Phyllobius sp. 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 1 0 0 0 1 Tanysphyrus lemnae* 0 0 0 0 0 0 0 Tychius picirostrus 1 0 0 1 0 0 2 Undetermined (Curculionidae) 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 Hygrotus sp. 1 1 0 1 0 1 4 Hygrotus!Hydroporus sp. 0 0 0 0 0 0 0 1/ybius sp. 0 1 0 0 0 0 1 Laccophilus sp. 1 1 0 0 0 0 2 Liodessus sp. 0 0 1 0 1 0 2 Neoporus sp. 0 0 0 0 0 0 0 Elmidae 393 Dubiraphia sp. 0 0 0 0 0 0 0 Optioservus sp. 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 Haliplidae Ha/iplus sp. 1 1 0 1 1 0 4 Peltodytes sp. 1 1 0 0 1 1 4 Hydraenidae Hydra ena sp. 0 0 1 1 0 0 2 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 Berosus sp. 0 0 0 0 0 0 0 Cymbiodyta sp. 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 0 0 0 Helophorus sp. 0 0 0 0 0 0 0 Hydrobius sp. 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 1 0 Scirtidae Scirtes sp. 1 0 0 0 0 0 Staphylinidae Pontamalota sp. 0 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 DiQtera (order} Undetermined (Diptera) 0 0 0 0 0 0 0 Ceratopogon idae Alluaudomyia sp. 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 Bezzia/Palpomyia sp. 1 0 1 1 1 0 4 C/inohelea!Heteromyia sp. 0 0 0 0 0 0 0 394 Cu/icoides sp. 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 Mallochohelea sp. 0 0 0 0 0 0 0 Sphaeromias sp. 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 1 0 1 1 0 0 3 Chironomidae Undetermined (Chironomidae) 1 1 1 1 1 1 6 Culicidae Aedes sp. 0 0 0 0 0 0 0 Aedes cinereus 0 0 0 0 0 0 0 Aedes vexans 1 0 0 0 0 0 1 Anopheles sp. 0 0 0 1 0 0 1 Anopheles earlei 0 0 0 1 0 0 1 Anopheles punctip ennis 0 0 0 0 0 0 0 Culex sp. 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 Dixidae Dixel/a sp. 0 0 0 0 0 0 0 Dolichopodidae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 Ephydridae Ephydra sp. 1 0 0 1 1 0 3 Hydrellia sp. 0 0 0 0 1 0 1 Setacera sp. 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 0 0 0 0 0 Psychoda sp. 0 0 0 0 0 0 0 Undetermined (Psychodidae) 1 0 0 0 0 0 1 395 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 1 0 0 1 Te tanocera sp. 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 Simuliidae Simulium sp. 0 0 0 0 0 0 0 Stratiomyidae Odontomyia sp. .o 0 1 0 0 0 1 Odontomyia!Hedriodiscus sp. 0 0 0 0 1 0 1 Oxycera sp. 0 0 1 0 0 0 1 Stratiomys sp. 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 0 0 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 Limnophila sp. 0 0 1 0 0 0 1 · Prionocera sp. 0 0 0 0 1 0 1 Undetermined (Tipulidae) 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 1 0 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Muscu/ium sp. 0 0 0 0 0 0 0 Pisidium sp. 0 0 0 0 0 0 0 Sphaerium sp. 0 1 0 0 0 0 1 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 Gastropoda (class) 396 BasommatoQhora (order} Undetermined (Gastropoda) 1 0 0 0 0 0 Ancylidae Ferrissia fra gilis 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicel/a 0 0 1 1 1 1 4 Pseudosuccinea columella 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 0 0 Stagnicola elodes 0 0 0 0 0 0 0 Physidae Aplexa elongata 1 0 0 0 0 0 1 Physella gyrina 1 1 1 1 1 1 6 Physel/a integra 0 0 0 0 0 0 0 Planorbidae Gyraulus deflectus 0 0 0 0 0 0 0 Gyra ulus parvus 0 1 0 1 1 1 4 Helisoma anceps 0 0 0 0 1 0 1 Planorbella trivolvis 0 1 0 1 0 0 2 Planorbula armigera 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 1 1 HeterostroQha (order) Valvatidae Va lvata tricarin ata 0 0 0 0 1 0 Neotaenioglossa (order} Hydrobiidae Amnicola walkeri 0 0 1 0 1 0 2 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdella punctata 0 1 0 0 1 0 2 Nephelopsis obscura 0 0 0 0 0 0 0 Rhynchobdellida (order) 397 Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 Desserobdella michiganensis 0 0 0 0 0 0 0 Gloiobdella elongata 0 0 0 0 0 0 0 G/ossiphonia complanata 0 0 0 0 0 0 0 Helobde/la fusca 0 0 0 0 0 0 0 Helobde/la stagnalis 0 1 0 0 0 0 1 Placobdella papil/ifera 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 1 1 1 1 6 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) Prostigmata (Suborder) Undetermined (Hydracarina) 1 1 1 1 1 1 6 CRUSTACEA (subphylum) Branchiopoda(cla ss) Cladocera (suborder) Undetermined ( Cladocera) 1 1 1 1 1 1 6 Ostracoda (class) Undetermined (Ostracoda) 1 1 1 1 1 1 6 Copepoda (subclass) Undetermined (Copepoda) 1 1 1 1 1 1 6 Malacostraca (class) lsogoda (order) Asellidae Caecidotea sp. 0 1 0 1 1 0 3 Amghigoda (order) Gammaridae Gammarus sp. 0 0 0 0 0 0 0 Hyalellidae Hya/lela azteca 1 1 1 1 1 0 5 398 Decapoda (order) Cambaridae Orconectes virilis 0 0 0 0 0 0 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0

Table 11. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1.4. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae. Only the presence (1) or absence (0) of each taxon is recorded.

Hardwood Creek: Site H-1 .3 (2004) Macroinvertebrates from SFPE samples

TAXA 4/28 5/26 6/23 7/28 9/1 10/6 11/13 Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined (Collembola) 1 1 1 1 1 1 1 7 Insecta (class) Ephemeroptera (order) Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 0 0 0 0 0 0 0 Callibaetis sp. 0 0 0 0 1 0 0 1 Labiobaetis sp. 0 0 0 1 1 1 1 4 Undetermined (Baetidae) 0 0 0 0 0 0 0 0 Caenidae Caenis sp. 1 1 0 1 1 1 1 6 Heptageniidae Heptagenia sp. 0 0 0 0 0 0 0 0 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 0 Odonata (order} 399 Calopterygidae Calopteryx sp. 0 0 0 0 0 0 0 0 Coenagrionidae Coenagrion/Enallagma sp. 1 0 0 1 1 1 1 5 lschnura sp. 0 0 0 0 1 1 0 2 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 0 Libellulidae Somatochlora sp. 0 0 0 0 0 0 0 0 Plecoptera (order) Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 0 Perlidae Perlesta sp. 0 0 0 0 0 0 0 0 Hemiptera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 1 0 0 1 0 2 Trichocorixa sp. 0 0 1 0 1 1 0 3 Undetermined (Corxidae immatures) 0 1 1 1 1 0 0 4 Gerridae Gerris sp. 0 0 0 1 0 0 0 1 Rheumatobates sp. 0 0 0 0 0 0 0 0 Hebridae Merragata sp. 0 0 0 0 1 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 0 Mesoveliidae Mesovelia sp. 0 0 1 1 1 1 0 4 Nepidae Ranatra sp. 0 0 0 0 0 0 0 0 400 Notonectidae Notonecta sp. 0 0 0 1 0 0 0 1 Pleidae Neoplea sp. 1 1 1 1 1 1 0 6 Veliidae Microvelia sp. 0 0 1 1 1 1 0 4 Megaloptera (order) Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 0 Trichoptera (order) Undetermined (Trichoptera) 1 0 0 0 1 0 0 2 Glossosomatidae Undetermined ( Glossosomatidae) 0 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 0 Hydroptilidae Agraylea sp. 0 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 1 0 1 0 2 Undetermined (Hydroptilidae) 0 0 0 1 0 0 0 1 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 0 Nectopsych e sp. 0 0 0 1 0 0 0 1 Oecetis sp. 0 0 0 0 1 0 0 1 Triaenodes sp. 0 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 0 Lepidoptera (order) Pyralidae Syn c/ita sp. 0 0 0 1 1 1 1 4 Coleoptera (order) Curculionidae 401 Lissorhpoterus simplex* 0 0 1 0 0 0 0 1 Listronotas angustus * 0 0 0 0 0 0 0 0 Phyllobius sp. 0 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus? 0 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 1 1 0 0 0 0 2 Tychius picirostrus 0 0 0 0 0 0 0 0 Undetermined (Curculionidae) 0 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 1 0 1 Hygrotus!Hydroporus sp. 0 0 0 0 0 0 0 0 1/ybius sp. 0 1 0 0 0 0 0 1 Laccophilus sp. 0 0 0 0 0 0 1 1 Liodessus sp. 0 1 0 0 0 1 0 2 Neoporus sp. 0 0 0 0 0 0 0 0 Elmidae Dubiraphia sp. 0 0 0 0 0 0 0 0 Optioservus sp. 0 0 0 0 0 0 0 0 Stene/mis sp. 0 0 0 0 0 0 0 0 Haliplidae Haliplus sp. 1 1 1 0 1 1 0 5 Peltodytes sp. 0 1 1 1 1 1 1 6 Hydraenidae Hydraena sp. 1 0 0 0 1 0 1 3 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 0 Berosus sp. 0 0 0 0 0 0 0 0 Cymbiodyta sp. 0 0 0 0 0 0 0 0 Enochrus sp. 1 0 0 1 0 0 0 2 He/ophorus sp. 0 0 0 0 0 0 0 0 402 Hydrobius sp. 0 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 1 0 0 0 0 Scirtidae Scirtes sp. 1 1 0 0 0 0 0 2 Staphylinidae Pontamalota sp. 0 0 0 0 0 1 0 1 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 0 Di(2tera (order} Undetermined (Diptera) 0 0 0 0 0 0 0 0 Ceratopogonidae Al/uaudomyia sp. 1 0 0 0 0 0 0 1 Atrichopogon sp. 0 0 0 0 0 0 0 0 Bezzia/Palpomyia sp. 1 1 0 0 0 0 0 2 Clinohelea/He teromyia sp. 0 0 0 0 0 0 0 0 Culicoides sp. 0 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 0 Ma/lochohelea sp. 0 0 0 0 0 0 0 0 Sphaeromias sp. 0 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 1 0 1 1 1 0 0 4 Chironomidae Undetermined (Chironomidae) 1 1 1 1 1 1 1 7 Culicidae Aedes sp. 1 1 0 0 0 0 0 2 - Aedes cinereus 1 1 0 0 0 0 0 2 Aedes vexans 1 1 0 0 0 0 0 2 Anopheles sp. 0 0 0 0 0 1 0 1 Anopheles earlei 0 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 0 0 0 0 403 Culex sp. 0 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 0 Dixidae Dixel/a sp. 0 0 0 0 1 0 0 Dolichopodidae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 0 Ephydridae Ep hydra sp. 0 0 0 0 0 0 0 0 Hydrellia sp. 0 0 0 0 0 0 0 0 Setacera sp. 0 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 0 0 1 0 0 1 Psychoda sp. 0 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 0 Te tanocera sp. 0 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 0 Simul iidae Simulium sp. 1 1 1 0 0 0 1 4 Stratiomyidae Odontomyia sp. 0 1 0 0 0 0 0 1 Odontomyia/Hedriodiscus sp. 1 0 1 0 1 0 0 3 Oxycera sp. 0 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 0 Syrphidae 404 Eristalis sp. 0 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 1 0 0 0 0 0 0 1 Tipulidae He/ius sp. 0 0 0 0 0 0 0 0 Limnophila sp. 0 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 1 0 0 0 0 0 1 MOLLUSCA (phylum) Bivalvia (class) Veneroida Corder) Pisidiidae Musculium sp. 0 0 0 0 0 0 0 0 Pisidium sp. 1 1 0 0 0 0 0 2 Sphaerium sp. 0 0 0 0 0 0 0 0 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 0 Gastropoda (class) BasommatoQhora (order} Undetermined (Gastropoda) 0 0 0 0 0 0 0 0 Ancylidae Ferrissia fra gilis 0 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicella 0 0 1 1 0 1 0 3 Pseudosuccinea columella 0 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 0 0 0 Stagnico/a elodes 0 0 0 0 0 0 0 0 Physidae Ap/exa e/ongata 1 0 0 0 0 0 0 1 Physella gyrina 0 1 1 1 1 1 0 5 Physel/a integra 0 0 0 0 0 0 0 0 Planorbidae 405 Gyra ulus def/ectus 0 0 0 0 0 0 0 0 Gyra ulus parvus 1 0 0 1 1 1 0 4 Helisoma anceps 0 0 0 0 0 0 0 0 Planorbella trivolvis 0 0 1 0 1 0 0 2 Planorbula armigera 0 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 0 Heterostropha (order) Valvatidae Va lvata tricarin ata 0 0 1 1 0 1 0 3 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 0 1 0 1 1 1 0 4 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobde/la punctata 0 0 0 1 0 0 0 1 Nephelopsis obscura 0 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 0 Desserobdella michiganensis 0 0 0 0 0 0 0 0 Gloiobdella elongata 0 0 0 0 0 0 0 0 Glossiphonia complanata 0 0 0 0 0 0 0 0 Helobdella fu sca 0 0 0 0 0 0 0 0 Helobdel/a stagnalis 0 0 0 0 0 0 0 0 Placobdella papillifera 0 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 1 1 1 1 1 7 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) 406 Prostigmata (Suborder) Undetermined (Hydracarina) 7 CRUSTACEA (subphylum) Branchiopoda(class) Cladocera (suborder) Undetermined (Ciadocera) 7 Ostracoda (class) Undetermined (Ostracoda) 0 6 Copepoda (subclass) Undetermined (Copepoda) 7 Malacostraca (class) lsopoda (order) Asellidae Caecidotea sp. 0 0 0 0 0 2 Amphipoda (order) Gammaridae Gammarus sp. 0 0 0 0 0 0 0 0 Hyalellidae Hya/lela azteca 0 0 0 4 Decapoda (order) Cambaridae Orconectes virilis 0 0 0 0 0 0 0 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0 0

Table 12. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1.3. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae. Only the presence (1) or absence (0) of each taxon is recorded.

407 Hardwood Creek: Site H-1.50 (2004) Macroinvertebrates from SFPE samples

TAXA 4/28 5/26 6/23 7/28 9/1 10/6 11/13 Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined (Collembola) 1 1 1 1 1 1 1 7 Insecta (class) EQhemeroQtera (order) Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 0 0 0 0 0 0 0 Callibaetis sp. 0 0 0 1 0 0 0 1 Labiobaetis sp. 0 0 0 0 0 0 0 0 Undetermined (Baetidae) 0 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 0 0 0 0 0 0 0 Heptageniidae Heptagenia sp. 0 0 0 0 0 0 0 0 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 0 Odonata (order) Calopterygidae Calopteryx sp. 0 0 0 0 0 0 0 0 Coenagrionidae Coenagrion!Enallagma sp. 0 0 0 1 1 1 0 3 lschnura sp. 0 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 0 Libellulidae Somatochlora sp. 1 1 0 0 0 0 0 2 PlecoQtera (order) 408 Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 0 Perlidae Perlesta sp. 0 0 0 .0 0 0 0 0 Hemiptera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 1 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 1 0 0 0 1 2 Undetermined (Corxidae immatures) 0 1 1 0 0 0 0 2 Gerridae Gerris sp. 0 1 0 0 0 0 0 1 Rheumatobates sp. 0 0 0 0 0 0 0 0 Hebridae Merragata sp. 0 0 0 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 1 0 0 0 Mesoveliidae Mesovelia sp. 0 1 1 0 1 1 0 4 Nepidae Ranatra sp. 0 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 0 Pleidae Neoplea sp. 1 1 1 1 0 1 0 5 Veliidae Micro velia sp. 1 1 1 1 0 1 0 5 Megaloptera (order) Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 0 409 Trichoptera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 0 Glossosomatidae Undetermined (Giossosomatidae) 0 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 0 Hydroptilidae Agraylea sp. 0 0 1 0 0 0 0 1 Orthotrichia sp. 0 0 0 1 0 0 0 1 Oxyethira sp. 0 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 1 0 0 0 0 1 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 0 Nectopsyche sp. 0 0 0 0 0 0 0 0 Oecetis sp. 0 0 0 1 1 0 0 2 Tria enodes sp. 0 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 1 0 Lepidoptera (order) Pyralidae Sync/ita sp. 1 0 0 0 1 1 0 3 Coleoptera (order) Curculionidae Lissorhpoterus simplex * 0 0 0 0 0 0 0 0 Listronotas angustus * 0 0 0 0 0 0 0 0 Phyllobius sp. 0 0 0 0 0 0 0 0 Po/ydrusus sericeus 0 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 1 0 0 0 0 0 1 Tychius picirostrus 0 0 0 0 0 0 0 0 Undetermined (Curculionidae) 0 0 0 0 0 0 0 0 Dytiscidae 410 Agabus sp. 0 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 1 1 Hygrotus sp. 0 0 0 0 1 0 0 1 Hygrotus!Hydroporus sp. 0 0 0 1 0 0 0 1 1/ybius sp. 1 1 0 1 1 1 0 5 Laccophilus sp. 0 0 0 0 0 0 0 0 Liodessus sp. 0 1 0 1 0 1 0 3 Neoporus sp. 1 0 0 0 0 1 0 2 Elmidae Dubiraphia sp. 0 0 0 0 0 0 0 0 Optioservus sp. 0 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 0 Haliplidae Haliplus sp. 1 1 1 1 0 1 0 5 Peltodytes sp. 1 0 0 0 0 0 0 1 Hydraenidae Hydraena sp. 0 0 0 1 0 0 0 Hydrophilidae Anacaena sp. 0 0 0 0 1 0 0 1 Berosus sp. 0 0 0 0 0 0 0 0 Cymbiodyta sp. 0 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 0 0 0 0 Helophorus sp. 0 0 0 0 0 0 0 0 Hydrobius sp. 0 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 1 1 0 0 2 Paracymus sp. 0 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 0 Tropisternus sp. 0 0 1 0 0 0 0 1 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 0 0 Scirtidae Scirtes sp. 1 1 0 0 0 0 0 2 411 Staphylinidae Pontamalota sp. 0 0 0 0 0 0 1 1 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 0 DiQtera (order} Undetermined (Diptera) 0 0 0 0 0 0 0 0 Ceratopogonidae Alluaudomyia sp. 0 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 0 Bezzia!Palp omyia sp. 1 1 1 0 0 0 0 3 Clinohelea!Heteromyia sp. 0 0 0 0 0 0 0 0 Culicoides sp. 0 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 0 Mallochohelea sp. 0 0 0 0 0 0 0 0 Sphaeromias sp. 0 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 1 1 0 0 0 0 0 2 Chironomidae Undetermined (Chironomidae) 1 1 1 1 1 0 1 6 Culicidae Aedes sp. 1 0 0 0 0 0 0 1 Aedes cinereus 1 0 0 0 0 0 0 1 Aedes vexans 0 0 0 0 0 0 0 0 Anopheles sp. 1 1 0 0 0 0 0 2 Anopheles earlei 0 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 0 1 0 1 Culex sp. 0 0 0 0 0 0 0 0 Culex territans 0 0 0 1 0 0 0 1 Culiseta inornata 1 0 0 0 0 0 0 1 Undetermined (Culicidae) 0 0 0 1 0 0 0 1 Dixidae Dixella sp. 0 0 0 0 0 0 0 0 Dolichopodidae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 0 Empididae 412 Chelifera sp. 0 0 0 0 0 0 0 0 Hemerodromia sp. 0 0 0 0 0 0 0 0 Ephydridae Ep hydra sp. 0 0 0 0 0 1 0 1 Hydrellia sp. 0 0 0 1 1 1 1 4 Setacera sp. 0 1 0 0 0 0 0 1 Psychodidae Pericoma sp. 0 0 0 0 0 0 0 0 Psychoda sp. 0 0 0 0 0 1 0 1 Undetermined (Psychodidae) 0 0 0 0 0 1 0 1 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 0 Sciomyzidae Antichaeta .sp. 0 0 0 0 1 0 0 1 Te tanocera sp. 0 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 0 Simuliidae Simulium sp. 1 1 0 0 0 0 1 3 Stratio myid ae Odontomyia sp. 1 0 0 0 0 0 0 1 Odontomyia!Hedriodiscus sp. 1 1 1 1 0 0 1 5 Oxycera sp. 0 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 1 0 0 0 Tipulidae • He/ius sp. 0 0 0 0 0 0 1 1 Limnophila sp. 0 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 0 NEMATODA (phylum) 413 Undetermined (Nematoda) 1 1 0 0 1 0 0 3 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Musculium sp. 0 0 0 0 0 0 0 0 Pisidium sp. 0 0 0 1 1 0 0 2 Sphaerium sp. 0 0 0 1 1 1 0 3 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 0 Gastropoda (class) BasommatoQhora (order) Undetermined (Gastropoda) 0 0 0 0 0 0 0 0 Ancylidae Ferrissia fra gilis 0 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicel/a 0 1 1 1 1 1 0 5 Pseudosuccinea columella 0 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 0 0 0 Stagnicola elodes 0 0 0 0 0 0 0 0 Physidae Aplexa elongata 0 0 0 0 0 0 0 0 Physella gyrina 1 1 1 1 1 1 0 6 Physetla integra 0 0 0 0 0 0 0 0 Planorbidae Gyra u/us deflectus 0 0 0 0 1 0 0 1 Gyraulus parvus 0 1 1 1 1 1 0 5 Helisoma anceps 0 0 0 0 1 0 0 1 P/anorbella trivolvis 0 1 1 1 1 1 ·0 5 Planorbula armigera 0 0 1 1 1 0 0 3 Promenetus exacuous 0 0 0 0 1 1 0 2 HeterostroQha (order) Valvatidae Valvata tricarin ata 0 0 0 0 1 0 0 414 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 0 0 0 1 1 1 0 3 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdella punctata 0 0 0 0 1 0 0 1 Nephelopsis obscura 0 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 1 0 1 Desserobdella michiganensis 0 0 0 0 0 0 0 0 Gloiobdella elongata 0 0 0 0 0 0 0 0 Glossiphonia complanata 0 0 0 0 1 0 0 1 Helobdella fu sca 0 0 0 0 1 0 0 1 Helobdella stagnalis 0 0 0 1 1 0 0 2 Placobdel/a papillifera 0 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 1 1 1 1 1 7 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) Prostigmata (Suborder) Undetermined (Hydracarina) 1 1 1 1 1 1 1 7 CRUSTACEA (subphylum) Branch iopoda(cl ass) Cladocera (suborder) Undetermined (Ciadocera) 1 1 1 1 1 1 1 7 Ostracoda (class) Undetermined (Ostracoda) 1 1 1 1 1 0 0 5 Copepoda (subclass) 415 Undetermined (Copepoda) 7 Malacostraca (class) lso12oda (order} Asellidae Caecidotea sp. 0 0 0 1 1 0 0 2 Am[2hipoda (order} Gammaridae Gammarus sp. 0 0 0 0 0 0 0 0 Hyalellidae Hyallela azteca 0 1 1 1 1 1 0 5 Deca12oda (order} Cambaridae Orconectes virilis 0 0 0 0 0 0 0 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0 0

Table 13. Cumulative taxa list fo r macroinvertebrates from SFPE samples at site 1.50. An asterisk (*) indicates aquatic or semi­ aquatic Curculionidae. Only the presence (1) or absence (0) of each taxon is recorded.

Hardwood Creek: Site H-1 (2004) Macroinvertebrates from SFPE samples

TAXA 4/28 5/26 6/23 7/28 9/1 10/6 Total ARTHROPODA (phylum) · Entognatha (class) Collembola (order) Undetermined ( Collembola) 0 0 4 Insecta (class) E12hemero12tera (order} Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 0 0 0 2 416 Cal/ibaetis sp. 0 0 0 0 0 0 0 Labiobaetis sp. 0 0 0 1 1 1 3 Undetermined (Baetidae) 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 1 0 1 0 0 2 Heptageni idae Heptagenia sp. 0 0 0 0 0 0 0 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 Odonata (order) Calopterygidae Calopteryx sp. 0 0 0 1 0 0 1 Coenagrionidae Coenagrion!Enallagma sp. 0 0 0 0 0 0 0 lschnura sp. 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 Libellulidae Somatochlora sp. 0 0 0 0 0 0 0 Plecoptera (order) Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 Perlidae Perle sta sp. 0 0 0 0 0 0 0 Hemiptera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 0 0 1 0 1 Undetermined (Corxidae immatures) 0 0 0 1 0 0 1 Gerridae 417 Gerris sp. 0 0 0 0 0 0 0 Rheumatobates sp. 0 0 0 1 0 0 1 Hebridae Merragata sp. 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 Mesoveliidae Mesovelia sp. 0 0 0 1 0 0 Nepidae Ranatra sp. 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 1 0 0 0 Pleidae Neoplea sp. 1 0 0 1 1 1 4 Veliidae Microvelia sp. 0 0 1 1 0 0 2 Megaloptera (order) Corydalidae Chau/iodes sp. 0 0 0 0 0 0 0 Trichoptera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 Glossosomatidae Undetermined ( Glossosomatidae) 0 0 0 0 0 0 0 Hydro psych idae Cheumatopsyche sp. 0 0 0 0 0 0 0 Hydroptilidae Agraylea sp. 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 Oxyethira sp. 1 0 0 0 0 0 1 Undetermined (Hydroptilidae) 0 0 0 0 0 0 0 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 Nectopsyche sp. 0 0 0 0 0 0 0 418 Oecetis sp. 0 0 0 0 0 0 0 Triaenodes sp. 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 Lepidoptera (order) Pyralidae Sync/ita sp. 0 0 0 0 0 1 1 Coleoptera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 Listronotas angustus* 0 0 0 0 0 0 0 Phyl/obius sp. 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 0 0 0 1 0 1 Tychius picirostrus 1 0 0 0 0 0 1 Undetermined (Curculionidae) 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 1 0 0 0 1 Hydroporus sp. 1 0 0 0 0 0 1 Hydro vatus sp. 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 Hygrotus!Hydroporus sp. 0 0 0 0 0 0 0 1/ybius sp. 0 0 0 1 0 0 1 Laccophilus sp. 0 0 0 0 0 0 0 Liodessus sp. 0 0 1 0 0 0 1 Neoporus sp. 0 0 0 0 0 0 0 Elmidae Dubiraphia sp. 0 0 0 1 0 0 1 Optioservus sp. 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 Haliplidae 419 Haliplus sp. 0 0 0 0 0 0 0 Peltodytes sp. 1 0 0 0 0 0 1 Hydraenidae Hydra ena sp. 0 0 0 0 0 Hydrophilidae Anacaena sp. 1 0 0 0 0 0 1 Berosus sp. 0 0 0 0 0 0 0 Cymbiodyta sp. 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 0 0 0 Helophorus sp. 0 0 0 0 0 1 1 Hydrobius sp. 0 0 0 0 0 0 0 Hydro chus sp. 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 Tropisternus sp. 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 0 0 0 Scirtidae Scirtes sp. 0 0 0 0 0 Staphylinidae Pontamalota sp. 0 0 0 0 0 0 0 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 DiQtera (order} Undetermined (Diptera) 0 0 0 0 0 0 0 Ceratopogonidae Alluaudomyia sp. 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 Bezzia/Palpomyia sp. 0 0 1 0 0 0 1 Clinohelea!Heteromyia sp. 0 0 0 0 0 0 0 Cu/icoides sp. 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 Mal/ochohelea sp. 0 0 0 0 0 0 0 Sphaeromias sp. 0 0 0 0 0 0 0 420 Undetermined (Ceratopogonidae) 1 0 0 0 0 0 Chironomidae Undetermined (Chironomidae) 0 1 0 1 1 1 4 Culicidae Aedes sp. 1 0 0 0 0 0 1 Aedes cinereus 0 0 0 0 0 0 0 Aedes vexans 0 0 0 0 0 0 0 Anopheles sp. 0 0 0 1 1 0 2 Anopheles earlei 0 0 0 1 0 0 1 Anopheles punctip ennis 0 0 0 1 1 1 3 Culex sp. 0 0 0 0 0 0 0 Culex territans 0 0 0 0 0 0 0 Culiseta inornata 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 Dixidae Dixella sp. 0 0 0 0 0 0 0 Dolichopodidae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 Hemerodromia sp. 0 1 0 0 0 0 1 Ephydridae Ephydra sp. 0 0 0 0 0 0 0 Hydrellia sp. 0 0 1 1 0 0 2 Setacera sp. 0 0 0 0 0 0 0 Psycho didae Pericoma sp. 0 0 0 0 0 0 0 Psychoda sp. 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 42 1 Te tanocera sp. 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 Simuliidae Simulium sp. 0 1 0 1 0 1 3 Stratiomyidae Odontomyia sp. 1 0 0 0 0 0 1 Odontomyia!Hedriodiscus sp. 0 0 0 0 0 0 0 Oxycera sp. 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 0 0 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 Limnophila sp. 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Musculium sp. . 0 0 0 0 0 0 0 Pisidium sp. 0 0 0 0 0 0 0 Sphaerium sp. 0 0 0 0 0 0 0 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 Gastropoda (class) BasommatoQhora (order} Undetermined (Gastropoda) 0 0 0 0 0 0 0 Ancylidae Ferrissia tra gi/is 0 0 0 0 0 0 0 422 Lymnaeidae Fossaria modicella 0 0 0 0 0 0 0 Pseudosuccinea columella 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 0 0 Stagnicola elodes 0 0 0 0 0 0 0 Physidae Aplexa elongata 0 0 0 0 0 0 0 Physel/a gyrina 0 0 0 1 0 0 1 Physella integra 0 0 0 0 0 0 0 Planorbidae Gyra ulus deflectus 0 0 0 0 0 0 0 Gyraulus parvus 0 0 0 0 0 1 1 Helisoma anceps 0 0 0 0 0 0 0 Planorbel/a trivolvis 0 0 0 0 0 0 0 Planorbula armigera 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 Heterostropha (order) Valvatidae Va lvata tricarinata 0 0 0 0 0 0 0 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 0 0 0 1 0 0 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdella punctata 0 0 0 0 0 0 0 Nephelopsis obscura 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae

Alboglossiphonia heteroclita 0 • 0 0 0 0 0 0 Desserobdella michiganensis 0 0 0 0 0 0 0 . Gloiobdella elongata 0 0 0 0 0 0 0 423 Glossiphonia complanata 0 0 0 0 0 0 0 He/obdel/a fusca 0 0 0 0 0 0 0 He/obdel/a stagnalis 0 0 0 0 0 0 0 Placobdel/a papil/ifera 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 0 0 0 1 0 1 2 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) Prostigmata (Suborder) Undetermined (Hydracarina) 0 0 0 1 1 1 3 CRUSTACEA (subphylum) Branch iopoda(cl ass) Cladocera (suborder) Undetermined ( Cladocera) 0 0 0 1 0 1 2 Ostracoda (class) Undetermined (Ostracoda) 0 0 0 1 0 1 2 Copepoda (subclass) Undetermined (Copepoda) 1 0 0 1 0 1 3 Malacostraca (class) lsoQoda (order} Asellidae Caecidotea sp. 0 0 0 0 0 0 0 AmQhiQoda (order) Gammaridae Gammarus sp. 0 0 0 0 0 0 0 Hyalellidae Hya llela azteca 0 1 0 1 0 1 3 DecaQoda (order} Cambaridae Orconectes virilis 0 0 0 0 0 0 0 Chordata (phylum) 424 Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0

Table 14. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae. Only the presence (1) or absence (0) of each taxon is recorded.

Hardwood Creek: Site H-1 .1 (2004) Macroinvertebrates from SFPE samples

TAXA 4/28 5/26 6/23 7/28 9/1 10/6 Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined ( Collembola) 1 1 1 1 1 1 6 Insecta (class) EQhemeroQtera (order} Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 1 0 0 1 0 2 Callibaetis sp. 0 0 0 0 0 0 0 Labiobaetis sp. 0 0 0 1 0 1 2 Undetermined (Baetidae) 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 1 0 1 0 0 2 Heptageniidae Heptagenia sp. 0 0 0 1 0 0 Leptophlebiidae Habrophlebiodes sp. 0 1 0 0 0 0 Odonata (order} Calopterygidae Calopteryx sp. 0 0 0 0 0 0 0 Goenagrionidae Coenagrion!Enallagma sp. 0 0 0 0 0 0 0 425 lschnura sp. 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 Libellulidae Somatochlora sp. 0 0 0 0 0 0 0 Plecoptera (order) Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 Perlidae Per/esta sp. 0 0 0 0 0 0 0 Hemiptera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 0 0 0 0 0 Undetermined (Corxidae immatures) 0 0 0 0 0 1 1 Gerridae Gerris sp. 0 0 0 0 0 0 0 Rheumatobates sp. 0 0 0 1 1 0 2 Hebridae Merragata sp. 0 0 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 Mesoveliidae Mesovelia sp. 0 0 0 1 0 0 Nepidae Ranatra sp. 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 Pleidae Neoplea sp. 1 0 0 1 0 1 3 426 Veliidae Microvelia sp. 0 1 0 1 0 0 2 Megaloptera (order) Corydalidae Chauliodes sp. 0 0 0 1 0 0 Trichoptera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 Glossosomatidae Undetermined ( Glossosomatidae) 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 Hydroptilidae Agray/ea sp. 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 0 0 0 0 0 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 Nectopsyche sp. 0 0 0 1 0 0 1 Oecetis sp. 0 0 0 0 0 0 0 Tria enodes sp. 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 0 0 Lepidoptera (order) Pyralidae Sync/ita sp. 0 0 0 1 0 1 2 Coleoptera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 Listronotas angustus* 0 0 0 1 0 0 1 Phyllobius sp. 0 0 0 0 0 0 0 Polydrusus sericeus 0 0 0 0 0 0 0 427 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 0 0 0 0 0 0 Tychius picirostrus 1 0 0 1 0 0 2 Undetermined (Curculionidae) 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 1 0 1 0 0 0 2 Hydroporus sp. 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 Hygrotus/Hydroporus sp. 0 0 0 0 0 0 0 1/ybius sp. 0 0 0 0 0 0 0 Laccophilus sp. 1 0 0 0 0 0 1 Liodessus sp. 1 1 0 0 1 1 4 Neoporus sp. 0 0 0 0 0 0 0 Elmidae Dubiraphia sp. 0 0 0 0 0 0 0 Optioservus sp. 0 0 0 0 0 0 0 Stenelmis sp. 0 0 0 0 0 0 0 Haliplidae Halip/us sp. 0 0 0 1 1 1 3 Peltodytes sp. 1 0 0 0 0 0 1 Hydraenidae Hydra ena sp. 0 0 0 1 0 0 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 Berosus sp. 1 0 0 0 0 0 1 Cymbiodyta sp. 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 1 0 0 1 Helophorus sp. 0 0 0 0 0 0 0 Hydrobius sp. 0 1 0 0 0 0 1 Hydrochus sp. 0 0 0 0 0 0 0 Paracymus sp. 0 0 0 0 0 0 0 Sperchopsis sp. 0 0 0 0 0 0 0 428 '\ Tropisternus sp. 0 0 ) 0 0 \} Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 0 0 0 Scirtidae .. Scirtes sp. 1 0 0 1 0 0 2 Staphylinidae Pontamalota sp. 0 0 1 0 1 0 2 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 Di(2tera (order} Undetermined (Diptera) 0 0 0 0 0 0 0 Ceratopogonidae Alluaudomyia sp. 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 Bezzia/Palpomyia sp. 0 1 1 0 0 0 2 C/inohelea!He teromyia sp. 0 0 0 0 0 0 0 Culicoides sp. 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 Mallochohelea sp. 0 0 0 0 0 0 0 Sphaeromias sp. 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 1 1 0 0 0 0 2 Chironomidae Undetermined (Chironomidae) 1 1 0 1 1 1 5 Culicidae Aedes sp. 0 0 0 0 0 0 0 Aedes cinereus 0 1 0 0 0 0 1 Aedes vexans 1 1 0 0 0 0 2 Anopheles sp. 0 1 0 1 1 1 4 Anopheles earlei 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 1 1 2 Culex sp. 0 0 0 0 1 0 1 Culex territans 0 0 0 1 1 0 2 Culiseta inornata 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 429 Dixidae Dixella sp. 0 0 0 0 0 0 0 Dolichopodidae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 Empididae Chelifera sp. 0 0 0 0 0 0 0 Hemerodromia sp. 0 1 0 0 0 0 1 Ephydridae Ep hydra sp. 1 0 0 0 0 0 1 Hydrellia sp. 1 0 0 0 1 1 3 Setacera sp. 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 1 0 0 0 1 Psychoda sp. 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 1 0 0 0 1 2 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 0 0 0 0 0 0 0 Te tanocera sp. 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 Simuliidae Simulium sp. 0 1 0 0 0 0 Stratiomyidae Odontomyia sp. 0 0 0 0 0 1 1 Odontomyia!Hedriodiscus sp. 1 1 0 1 1 0 4 Oxycera sp. 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 0 0 0 0 Tipulidae 430 He/ius sp. 0 0 0 0 0 0 0 Limnophila sp. 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Muscu/ium sp. 0 0 0 0 0 0 0 Pisidium sp. 0 0 0 0 0 1 1 Sphaerium sp. 0 0 0 0 0 0 0 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 Gastropoda (class) Basommato�hora (order) Undetermined (Gastropoda) 0 0 0 1 0 0 Ancylidae Ferrissia fra gilis 0 0 0 1 0 0 Lymnaeidae Fossaria modicel/a 0 0 0 1 0 0 1 Pseudosuccinea columella 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 0 0 Stagnicola e/odes 0 1 0 0 0 0 1 Physidae Aplexa elongata 1 1 0 0 0 0 2 Physella gyrina 0 1 0 1 0 0 2 Physella integra 0 0 0 0 0 0 0 Planorbidae Gyrau/us def/e ctus 0 0 0 0 0 0 0 Gyraulus parvus 0 0 0 0 0 0 0 Helisoma anceps 0 0 0 0 0 0 0 Planorbel/a trivo/vis 0 0 0 0 0 0 0 43 1 Planorbula armigera 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 Heterostropha (order) Valvatidae Va lvata tricarin ata 0 0 0 0 0 0 0 Neotaenioqlossa (order) Hydrobiidae Amnicola walkeri 0 0 0 0 0 0 0 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdella punctata 0 0 0 0 0 0 0 Nephelopsis obscura 0 0 0 1 0 0 1 Rhynchobdellida (order) Glossiphoniidae Albog/ossiphonia heteroclita 0 0 0 0 0 0 0 Desserobdella michiganensis 0 0 0 0 0 0 0 Gloiobdella elongata 0 0 0 0 0 0 0 G/ossiphonia complanata 0 0 0 0 0 0 0 Helobdella fusca 0 0 0 0 0 0 0 Helobdella stagnalis 0 0 0 0 0 0 0 Placobdella papil/ifera 0 0 0 0 0 0 0 Th eromyzon biannu/atum 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 0 1 0 1 4 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) Prostigmata (Suborder) Undetermined (Hydracarina) 1 1 0 1 1 1 5 CRUSTACEA (subphylum) Branchiopoda(cl ass) 432 Cladocera (suborder) Undetermined {Ciadocera) 0 0 0 3 Ostracoda {class) Undetermined (Ostracoda) 0 0 0 0 0 0 0 Copepoda {subclass) Undetermined (Copepoda) 0 0 4 Malacostraca {class) lsopoda (order) Asellidae Caecidotea sp. 0 0 0 0 0 0 0 Amphipoda (order) Gammaridae Gammarus sp. 0 0 0 0 0 1 Hyalellidae Hya l/ela azteca 0 0 0 3 Decapoda (order) Cambaridae Orconectes virilis 0 0 0 0 0 0 0 Chordata (phylum) Osteichthyes {class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0

Table 15. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1.1. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae. Only the presence (1) or absence (0) of each taxon is recorded.

Hardwood Creek: Site H-1.2 (2004) Macroinvertebrates from SFPE samples

TAXA 4/28 5/26 6/23 7/28 9/1 1 0/6 Total ARTHROPODA {phylum) Entognatha {class) Collembola (order) Undetermined (Collembola) 1 1 0 1 1 1 5 433 Insecta (class) E(2hemero(2tera (order) Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 Baetidae Baetis sp. 1 1 0 0 1 0 3 Cal/ibaetis sp. 0 0 0 0 0 0 0 Labiobaetis sp. 0 0 1 1 0 1 3 Undetermined (Baetidae) 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 1 0 1 0 0 2 Heptageniidae Heptagenia sp. 0 0 0 1 0 0 Leptophlebiidae Habrophlebiodes sp. 0 0 0 0 0 0 0 Odonata (order) Calopterygidae Calopteryx sp. 0 0 0 0 0 0 0 Coenagrion idae Coenagrion!Enallagma sp. 0 0 0 0 0 0 0 /schnura sp. 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 Libellulidae Somatochlora sp. 0 0 0 0 0 0 0 Pleco(2tera (order) Capniidae Undetermined (Capniidae) 1 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 1 0 0 0 0 0 Perlidae Perlesta sp. 0 0 0 0 0 0 0 Hemi(2tera (order) Belostomatidae Belostoma sp. 0 0 0 0 0 0 0 434 Corixidae Sigara sp. 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 0 0 0 0 0 Undetermined (Corxidae immatures) 0 0 0 1 0 0 1 Gerridae Gerris sp. 0 0 0 1 0 0 1 Rheumatobates sp. 0 0 0 1 0 1 2 Hebridae Merragata sp. 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 Mesoveliidae Mesove/ia sp. 0 0 0 1 0 0 Nepidae Ranatra sp. 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 Pleidae Neoplea sp. 0 0 0 1 0 0 Veliidae Microvelia sp. 0 0 0 0 0 0 0 Meqaloptera (order) Corydalidae Chauliodes sp. 0 0 0 0 0 0 0 Trichoptera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 Glossosomatidae Undetermined (Giossosomatidae) 1 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 1 0 0 0 0 1 2 Hydroptilidae Agray/ea sp. 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 435 Oxyethira sp. 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 0 0 0 0 0 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 Nectopsyche sp. 0 0 1 1 0 0 2 Oecetis sp. 0 0 0 0 0 0 0 Tria enodes sp. 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 1 0 0 0 0 0 Lepidoptera (order) Pyralidae Syn c/ita sp. 0 0 0 0 0 Coleoptera (order) Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 Listronotas angustus * 0 0 0 0 0 0 0 Phyl/obius sp. 0 1 0 0 0 0 1 Po/ydrusus sericeus 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 0 0 0 0 0 0 Tychius picirostrus 0 0 0 0 1 0 1 Undetermined (Curculionidae) 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 Hygrotus/Hydroporus sp. 0 0 0 0 0 0 0 1/yb ius sp. 0 0 0 0 0 0 0 Laccophilus sp. 0 0 0 0 0 0 0 Liodessus sp. 0 0 0 0 0 1 1 Neoporus sp. 0 0 0 0 0 0 0 436 Elmidae Oubiraphia sp. 0 0 0 1 0 0 1 Optioservus sp. 1 0 0 0 0 0 1 Stenelmis sp. 0 1 0 0 0 0 1 Haliplidae Ha/ip/us sp. 0 0 0 0 0 0 0 Peltodytes sp. 1 0 0 0 0 0 1 Hydraenidae Hydraena sp. 0 0 0 1 1 1 3 Hydrophilidae Anacaena sp. 0 0 0 0 0 0 0 Berosus sp. 0 0 0 0 0 0 0 Cymbiodyta sp. 0 0 0 0 0 0 0 Enochrus sp. 1 0 0 0 0 0 1 Helophorus sp. 0 0 1 1 0 0 2 Hydrobius sp. 0 0 0 0 0 0 0 Hydrochus sp. 0 0 0 0 0 0 0 Paracymus sp. 1 0 0 0 0 0 1 Sperchopsis sp. 0 1 0 0 0 0 1 Tropisternus sp. 0 0 0 0 1 0 1 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 1 0 Scirtidae Scirtes sp. 0 0 0 0 0 0 0 Staphylinidae Pontamalota sp. 1 1 0 0 1 0 3 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 Dir;2tera (order} Undetermined (Diptera) 0 0 0 1 0 0 Ceratopogonidae Alluaudomyia sp. 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 Bezzia/Palpomyia sp. 0 0 0 0 0 0 0 437 Clinohelea/Heteromyia sp. 0 0 0 0 0 0 0 Culicoides sp. 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 Mallochohelea sp. 0 0 0 0 0 0 0 Sphaeromias sp. 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 0 0 0 1 1 0 2 Chironomidae Undetermined (Chironomidae) 1 1 0 1 1 1 5 Culicidae Aedes sp. 0 0 0 0 0 0 0 Aedes cinereus 0 0 0 0 0 0 0 Aedes vexans 0 0 0 0 0 0 0 Anopheles sp. 0 0 0 1 1 1 3 Anopheles earlei 0 0 0 0 0 0 0 Anopheles punctip ennis 0 0 0 0 1 1 2 Culex sp. 0 0 0 0 1 0 1 Culex territans 0 0 0 1 1 0 2 Culiseta inornata 0 0 0 0 0 0 0 Undetermined (Culicidae) 1 0 0 0 0 0 1 Dixidae Dixella sp. 1 0 0 0 0 0 Dol ichopod idae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 Empididae Chelifera sp. 1 0 0 0 0 0 Hemerodromia sp. 0 1 0 0 0 0 Ephydridae Ephydra sp. 1 0 0 0 0 0 1 Hydrel/ia sp. 0 0 0 0 1 0 1 Setacera sp. 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 1 0 0 0 1 Psychoda sp. 0 0 0 0 0 0 0 438 Undetermined (Psychodidae) 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 0 0 Sciomyzidae Antichaeta sp. 1 0 0 0 0 1 2 Te tanocera sp. 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 Simuliidae Simulium sp. 1 1 0 0 0 0 2 Stratiomyidae Odontomyia sp. 1 0 1 0 0 0 2 Odontomyia!Hedriodiscus sp. 0 0 1 0 0 0 1 Oxycera sp. 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 0 0 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 Limnophi/a sp. 0 0 0 0 0 0 0 Prionocera sp. 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Musculium sp. 0 0 0 0 0 0 0 Pisidium sp. 0 0 0 0 0 0 0 Sphaerium sp. 0 0 0 0 0 0 0 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 439 Gastropoda (class) Basommato(2hora(order) Undetermined (Gastropoda) 0 0 0 0 0 0 0 Ancylidae Ferrissia tra gi/is 0 0 0 1 0 0 Lymnaeidae Fossaria modicella 0 0 0 1 0 1 2 Pseudosuccinea columella 0 0 0 0 0 0 0 Stagnicola caperata 0 0 1 0 0 0 1 Stagnicola elodes 0 0 0 0 0 0 0 Physidae Aplexa elongata 1 0 0 0 0 0 1 Physel/a gyrina 0 0 0 1 0 1 2 Physel/a integra 0 0 0 1 0 0 1 Planorbidae Gyra ulus deflectus 0 0 0 0 0 0 0 Gyra ulus parvus 0 0 0 0 0 0 0 Helisoma anceps 0 0 0 0 0 0 0 Planorbella trivolvis 0 0 0 0 0 0 0 Planorbula armigera 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 Heterostro(2ha (order) Valvatidae Va lvata tricarin ata 0 0 0 0 0 0 0 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 0 0 0 0 0 0 0 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdel/a punctata 0 0 0 0 0 0 0 Nephelopsis obscura 0 0 0 0 0 0 0 440 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 Desserobdella michiganensis 0 0 0 0 0 0 0 Gloiobdella elongata 0 0 0 0 0 0 0 Glossiphonia complanata 0 0 0 0 0 0 0 Helobdel/a fusca 0 0 0 0 0 0 0 Helobdel/a stagnalis 0 0 0 0 0 0 0 Placobdella papi/lifera 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 0 0 Oligochaeta (subclass) Undetermined (Oiigochaeta) 1 1 0 1 0 0 3 ARTHROPODA (phylum) CHELICERATA (subphylum) Arachnida (class) Prostigmata (Suborder} Undetermined (Hydracarina) 1 1 0 1 1 1 5 CRUSTACEA (subphylum) Branch iopoda( class) Cladocera (suborder} Undetermined (Ciadocera) 0 0 0 1 0 0 Ostracoda (class) Undetermined (Ostracoda) 0 0 0 0 0 0 0 Copepoda (subclass) Undetermined (Copepoda) 0 1 0 1 0 0 2 Malacostraca (class) lso[2oda (order} Asellidae Caecidotea sp. 0 0 0 0 0 0 0 Am[2hi[2oda (order} Gammaridae Gammarus sp. 1 0 0 0 0 0 Hyalellidae 44 1 Hyallela azteca 0 1 0 1 0 1 3 Decapoda (order) Cambaridae Orconectes virilis 0 0 0 0 0 0 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0

Table 16. Cumulative taxa list for macroinvertebrates from SFPE samples at site 1.2. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae. Only the presence (1) or absence (0) of each taxon is recorded.

Hardwood Creek: Site H-2 (2004) Macroinvertebrates from SFPE samples

TAXA 4/28 5/26 6/23 7/28 9/1 10/6 Total ARTHROPODA (phylum) Entognatha (class) Collembola (order) Undetermined ( Collembola) 1 1 1 1 1 1 6 Insecta (class) Ephemeroptera (order} Undetermined (Ephemeroptera) 0 0 0 0 0 0 0 Baetidae Baetis sp. 0 1 0 0 1 0 2 Callibaetis sp. 0 0 0 0 0 0 0 Labiobaetis sp. 0 1 0 1 0 1 3 Undetermined (Baetidae) 0 0 0 0 0 0 0 Caenidae Caenis sp. 0 1 0 1 1 0 3 Heptageniidae Heptagenia sp. 0 0 0 1 0 1 2 Leptophlebiidae Habroph/ebiodes sp. 0 0 0 0 0 0 0 442 Odonata (order) Calopterygidae Ca/opteryx sp. 0 0 0 0 0 0 0 Coenagrionidae Coenagrion!Enallagma sp. 0 0 0 0 0 0 0 lschnura sp. 0 0 0 0 0 0 0 Undetermined (Coenagrionidae) 0 0 0 0 0 0 0 Libellulidae Somatochlora sp. 0 0 0 0 0 0 0 Plecoptera (order) Capniidae Undetermined (Capniidae) 0 0 0 0 0 0 0 Nemouridae Undetermined (Nemouridae) 0 0 0 0 0 0 0 Perlidae Perlesta sp. 0 0 0 0 0 0 0 Hemiptera (order) Belostomatidae Be/ostoma sp. 0 0 0 0 0 0 0 Corixidae Sigara sp. 0 0 0 0 0 0 0 Trichocorixa sp. 0 0 0 0 0 0 0 Undetermined (Corxidae immatures) 0 0 0 1 0 0 1 Gerridae Gerris sp. 0 0 0 1 0 0 Rheumatobates sp. 0 0 0 0 0 1 Hebridae Merragata sp. 0 0 0 0 0 Hydrometridae Hydrometra sp. 0 0 0 0 0 0 0 Mesoveliidae Mesovelia sp. 0 0 0 0 0 0 0 Nepidae 443 Ranatra sp. 0 0 0 0 0 0 0 Notonectidae Notonecta sp. 0 0 0 0 0 0 0 Pleidae Neoplea sp. 1 1 0 1 0 0 3 Veliidae Micro velia sp. 0 0 0 1 0 0 Megaloptera (order) Corydalidae Chau/iodes sp. 0 0 0 0 0 0 0 Trichoptera (order) Undetermined (Trichoptera) 0 0 0 0 0 0 0 Glossosomatidae Undetermined (Giossosomatidae) 0 0 0 0 0 0 0 Hydropsychidae Cheumatopsyche sp. 0 0 0 0 0 0 0 Hydroptilidae Agraylea sp. 0 0 0 0 0 0 0 Orthotrichia sp. 0 0 0 0 0 0 0 Oxyethira sp. 0 0 0 0 0 0 0 Undetermined (Hydroptilidae) 0 0 0 0 0 0 0 Leptoceridae Leptocerus sp. 0 0 0 0 0 0 0 Nectopsyche sp. 0 0 1 1 0 0 2 Oecetis sp. 0 0 0 0 0 0 0 Tria enodes sp. 0 0 0 0 0 0 0 Undetermined (Leptoceridae) 0 0 0 0 0 0 0 Limnephilidae Asynarchus sp. 0 0 0 0 0 Lepidoptera (order) Pyralidae Sync/ita sp. 0 0 0 0 0 0 0 Coleoptera (order) 444 Curculionidae Lissorhpoterus simplex* 0 0 0 0 0 0 0 Listronotas angustus * 0 0 0 0 0 0 0 Phyllobius sp. 0 0 0 0 0 0 0 Po/ydrusus sericeus 0 0 0 0 0 0 0 Tanymecus sp. nr. confertus ? 0 0 0 0 0 0 0 Tanysphyrus lemnae* 0 0 0 0 0 0 0 Tychius picirostrus 0 0 0 0 0 0 0 Undetermined (Curculionidae) 0 0 0 0 0 0 0 Dytiscidae Agabus sp. 0 0 0 0 0 0 0 Hydroporus sp. 0 0 0 0 0 0 0 Hydro vatus sp. 0 0 0 0 0 0 0 Hygrotus sp. 0 0 0 0 0 0 0 Hygrotus/Hydroporus sp. 0 0 0 0 0 0 0 1/ybius sp. 0 0 0 0 0 0 0 Laccophilus sp. 0 0 0 0 0 0 0 Liodessus sp. 0 1 0 0 1 1 3 Neoporus sp. 0 0 0 0 0 0 0 Elmidae Dubiraphia sp. 0 0 0 1 0 0 1 Optioservus sp. 0 0 0 0 0 0 0 Stenelmis sp. 0 1 0 0 0 0 1 Haliplidae Haliplus sp. 0 0 0 0 1 0 1 Peltodytes sp. 0 0 0 0 0 0 0 Hydraenidae Hydra ena sp. 0 1 0 1 1 0 3 Hydrophilidae Anacaena sp. 0 0 0 1 1 0 2 Berosus sp. 0 0 0 0 0 0 0 Cymbiodyta sp. 0 0 0 0 0 0 0 Enochrus sp. 0 0 0 0 1 1 2 445 Helophorus sp. 0 0 0 0 1 0 1 Hydrobius sp. 0 1 0 0 0 0 1 Hydro chus sp. 0 0 1 0 1 0 2 Paracymus sp. 0 0 0 0 0 0 0 Sperchopsis sp. 0 1 0 0 0 0 1 Tropisternus sp. 0 0 0 0 0 0 0 Ptiliidae Undetermined (Ptiliidae) 0 0 0 0 0 1 1 Scirtidae Scirtes sp. 0 1 0 0 0 0 Staphylinidae Pontamalota sp. 1 0 0 0 1 0 2 Undetermined (Staphylinidae) 0 0 0 0 0 0 0 Di[2tera (order) Undetermined (Diptera) 0 0 0 0 0 0 0 Ceratopogonidae Alluaudomyia sp. 0 0 0 0 0 0 0 Atrichopogon sp. 0 0 0 0 0 0 0 Bezzia/Palpomyia sp. 0 1 0 0 0 1 2 Clinohelea!Heteromyia sp. 0 0 0 0 0 0 0 Culicoides sp. 0 0 0 0 0 0 0 Dasyhelea sp. 0 0 0 0 0 0 0 Mallochohelea sp. 0 0 0 0 0 0 0 Sphaeromias sp. 0 0 0 0 0 0 0 Undetermined (Ceratopogonidae) 1 1 0 1 0 0 3 Chironomidae Undetermined (Chironomidae) 1 1 0 1 1 1 5 Culicidae Aedes sp. 0 1 0 0 0 0 1 Aedes cinereus 0 1 0 0 0 0 1 Aedes vexans 0 1 0 0 0 0 1 Anopheles sp. 0 0 0 1 0 1 2 Anopheles earlei 0 0 0 0 0 0 0 446 Anopheles punctipennis 0 0 0 1 1 1 3 Culex sp. 0 0 0 0 0 0 0 Culex territans 0 0 0 1 1 0 2 Culiseta inornata 0 0 0 0 0 0 0 Undetermined (Culicidae) 0 0 0 0 0 0 0 Dixidae Dixel/a sp. 0 0 0 0 0 0 0 Dol ichopod idae Undetermined (Dolichopodidae) 0 0 0 0 0 0 0 Empididae Chelifera s p. 0 0 0 0 0 0 0 Hemerodromia sp. 0 1 0 0 0 0 1 Ephydridae Ep hydra sp. 0 0 0 0 0 1 1 Hydrellia sp. 0 0 0 0 1 0 1 Setacera sp. 0 0 0 0 0 0 0 Psychodidae Pericoma sp. 0 0 1 0 0 1 2 Psychoda sp. 0 0 0 0 0 0 0 Undetermined (Psychodidae) 0 0 0 0 0 0 0 Sciaridae Undetermined (Sciaridae) 0 0 0 0 0 Sciomyzidae Antichaeta sp. 1 0 0 0 0 0 1 Tetanocera sp. 0 0 0 0 0 0 0 Undetermined (Sciomyzidae) 0 0 0 0 0 0 0 Simuliidae Simulium sp. 0 1 0 0 0 0 Stratiomyidae Odontomyia sp. 0 1 0 0 0 0 1 Odontomyia!He driodiscus sp. 0 1 1 0 1 0 3 Oxycera sp. 0 0 0 0 0 0 0 Stratiomys sp. 0 0 0 0 0 0 0 447 Syrphidae Eristalis sp. 0 0 0 0 0 0 0 Tabanidae Chrysops sp. 0 0 0 1 0 0 Tipulidae He/ius sp. 0 0 0 0 0 0 0 Limnophila sp. 0 1 0 0 0 0 1 Prionocera sp. 0 0 0 0 0 0 0 Undetermined (Tipulidae) 0 0 0 0 0 0 0 NEMATODA (phylum) Undetermined (Nematoda) 0 0 0 0 0 0 0 MOLLUSCA (phylum) Bivalvia (class) Veneroida (order) Pisidiidae Musculium sp. 0 0 0 0 0 0 0 Pisidium sp. 0 0 0 0 0 0 0 Sphaerium sp. 0 0 0 0 0 0 0 Undetermined (Pisidiidae) 0 0 0 0 0 0 0 Gastropoda (class) Basommato12bora (order} Undetermined (Gastropoda) 0 0 0 1 0 0 Ancylidae Ferrissia fra gilis 0 0 0 0 0 0 0 Lymnaeidae Fossaria modicel/a 0 1 0 0 1 1 3 Pseudosuccinea columella 0 0 0 0 0 0 0 Stagnicola caperata 0 0 0 0 0 0 0 Stagnicola e/odes 0 0 0 0 0 0 0 Physidae Ap/exa e/ongata 0 0 0 0 0 0 0 Physella gyrina 0 1 0 0 0 0 1 Physella integra 0 0 0 0 0 0 0 448 Planorbidae Gyra ulus def/ectus 0 0 0 0 0 0 0 Gyraulus parvus 0 0 0 0 0 0 0 Helisoma anceps 0 0 0 0 0 0 0 Planorbella trivolvis 0 0 0 0 0 0 0 Planorbula armigera 0 0 0 0 0 0 0 Promenetus exacuous 0 0 0 0 0 0 0 Heterostropha (order) Valvatidae Va lvata tricarin ata 0 0 0 0 0 0 0 Neotaenioglossa (order) Hydrobiidae Amnicola walkeri 0 0 0 0 0 0 0 ANNELIDA (phylum) Hirudinea (subclass) Arhynchobdellida (order) Erpobdellidae Erpobdella punctata 0 0 0 0 0 0 0 Nephelopsis obscura 0 0 0 0 0 0 0 Rhynchobdellida (order) Glossiphoniidae Alboglossiphonia heteroclita 0 0 0 0 0 0 0 Desserobdella michiganensis 0 0 0 0 0 0 0 Gloiobde/la elongata 0 0 0 0 0 0 0 Glossiphonia complanata 0 0 0 0 0 0 0 Helobdella fusca 0 0 0 0 0 0 0 Helobde/la stagnalis 0 0 0 0 0 0 0 Placobde/la papillifera 0 0 0 0 0 0 0 Th eromyzon biannulatum 0 0 0 0 0 1 1 Oligochaeta (subclass) Undetermined (Oiigochaeta) 0 1 0 1 0 1 3 ARTHROPODA (phylum) CHELICERATA (subphylum) 449 Arachnida (class) Prostigmata (Suborder} Undetermined (Hydracarina) 0 1 0 1 1 1 4 CRUSTACEA (subphylum) Branch iopoda(cl ass) Cladocera (suborder) Undetermined (Ciadocera) 0 0 0 1 0 1 2 Ostracoda (class) Undetermined (Ostracoda) 0 1 0 0 0 0 Copepoda (subclass) Undetermined (Copepoda) 0 1 0 1 0 1 3 Malacostraca (class) lsoQoda (order} Asellidae Caecidotea sp. 0 0 0 0 0 0 0 AmQhiQoda (order} Gammaridae Gammarus sp. 0 0 0 0 0 0 0 Hyalellidae Hyallela azteca 0 1 0 1 0 0 2 DecaQoda (order} Cambaridae Orconectes virilis 0 0 0 0 0 0 0 Chordata (phylum) Osteichthyes (class) Undetermined (Osteichthyes) 0 0 0 0 0 0 0

Table 17. Cumulative taxa list for macroinvertebrates from SFPE samples at site 2. An asterisk (*) indicates aquatic or semi-aquatic Curculionidae. Only the presence (1) or absence (0) of each taxon is recorded.

450 Hardwood Creek: Site H-1 .5U (2004) Chironomidae SFPE identified with dissection microscope

Taxon "' 4/28 5/26 6/23 7/28 9/1 10/6 11/4 Total Diamesinae Diamesa sp. 0 0 0 0 0 0 0 0 Pagastia sp. 0 0 0 0 0 0 0 0 Prodiamesinae Odontomesa sp. 0 0 0 0 0 0 0 0 Prodiamesa sp. 0 0 0 0 0 0 0 0 Orthocladiinae Acricotopus sp. 6 7 0 0 2 0 0 15 Brillia sp. (small, light & w/ short RO) 0 0 0 0 0 0 0 0 Brillia sp. (large, dark & w/ long RO) 0 0 0 0 0 0 0 0 Cardiocladius sp. 0 0 0 0 0 0 0 0 Chaetocladius sp. (weak, elongate AMS) 0 0 0 0 0 0 0 0 Chaetocladius sp. (strong, short AMS) 0 0 0 0 0 0 0 0 Corynoneura sp. (full AFS) 41 12 2 0 0 7 0 62 Corynoneura sp. (AFS in distal one-half)) 0 0 0 0 0 0 0 0 Cricotopus (lsocladius) sp. 45 5 3 0 . 0 14 3 70 Cricotopus sp. (w/o RO) 0 0 0 0 0 0 0 0 Cricotopus sp. (w/ RO & PSB on T2) 0 0 0 0 0 6 8 14 Cricotopus sp. (w/ RO & PSB on T2 & T3) 0 4 0 0 0 0 0 4 Cricotopus sp. (large, clear sp. w/ large RO & PSB only on T2) 0 0 0 0 0 0 0 0 Cricotopus trifascia 0 0 0 0 0 0 0 0 Diplocladius cultriger 0 0 0 0 0 0 0 0 Doncricotopus sp. 0 0 0 0 0 0 0 0 Eukiefferiella sp. (darker species, w/ RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (lighter species w/ smaller RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (big, light golden & w/o RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (Large, dark gold/brown, tapering RO w/ drawn out tip) 0 0 0 0 0 0 0 0 Heleniella sp. 0 0 0 0 0 0 0 0 Heterotrissocladius sp. 0 0 0 0 0 0 0 0 Hudsonimyia sp. 0 0 0 0 0 0 0 0 45 1 Hydrobaenus sp. 0 0 0 0 0 0 0 0 Krenosmittia sp. 0 0 0 0 0 0 0 0 Limnophyes sp. 6 0 0 0 1 0 0 7 Lopescladius sp. 0 0 0 0 0 0 0 0 Metriocnemus sp. 0 0 0 0 0 0 0 0 Nanocladius sp. (digitiform RO) 10 21 0 0 0 0 0 .31 Nanocladius sp. (pointed RO) 0 0 0 0 0 0 0 0 Nanocladius sp. (large bulbous RO w/ scales at tip) 0 0 0 0 0 0 0 0 Orthocladius (Eudactylocladius) sp. 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/ setal fringe) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/o setal fringe) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (PSB on T2 & T3) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) cf. frigidus 0 0 0 0 0 0 0 0 Orthocladius (Symposiocladius) cf. lignicola 0 0 0 0 0 0 0 0 Parachaetocladius sp. 0 0 0 0 0 0 0 0 Paracricotopus sp. 0 0 0 0 0 0 0 0 Parakiefferiella sp 0 0 0 0 0 0 0 0 Parametriocnemus sp. (dark form) 0 0 0 0 0 0 0 0 Parametriocnemus sp. (light form) 0 0 0 0 0 0 0 0 Paraphaenocladius sp. 2 0 0 0 0 0 0 2 Psectrocladius sp. 1 0 0 0 0 0 0 1 Pseudosmittia sp. 5 0 0 0 0 0 0 5 Psilometriocnemus sp. 0 0 0 0 0 0 0 0 Rheocricotopus sp. 0 0 0 0 0 0 0 0 Rheosmittia sp. 0 0 0 0 0 0 0 0 Smittia sp. 0 0 0 0 0 0 0 0 Synorthocladius sp. 0 0 0 0 0 0 0 0 Thienemanniella sp. 0 1 2 0 0 1 0 4 Tvetenia sp. (dark form) 0 0 0 0 0 0 0 0 Tvetenia sp. (golden form) 0 0 0 0 0 0 0 0 Tvetenia sp. (very clear form) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae (near Stilocladius) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus #2 (Like Paraphaenocladius but w/ one AMS) 0 0 0 0 0 0 0 0 Chironomini Chironomus sp. (w/o warts) 0 6 5 0 4 0 0 15 452 Chironomus sp. (w/ warts on frontal tubercles) 0 0 0 0 0 0 0 0 Cladopelma sp. 0 23 0 0 0 0 0 23 Cryptochironomus (golden, large fused CT, bifid at very tip of CT) 0 0 0 0 0 0 0 0 Cryptochironomus (darker, CT rounded & bulbous at base w/ 2 spine-like branches) 0 1 0 0 0 0 0 1 Cryptochironomus (light golden, CT not fused at base) 0 0 0 0 0 0 0 0 Cryptochironomus (golden, CT fused & bulbous, tips like pronghorn) 0 0 0 0 0 0 0 0 Cryptochironomus (dark golden, CT not fused, very large sp.) 0 0 2 0 0 0 0 2 Cryptochironomus (darker brown, CT not fused at base) 0 0 0 0 0 0 0 0 Cryptotendipes sp. 0 0 0 0 0 0 0 0 Demeijeria sp. 0 0 0 0 0 0 0 0 Dicrotendipes fumidus 0 0 1 0 0 0 0 1 Dicrotendipes sp. (w/ sternal spines) 0 0 1 0 0 0 0 1 Endochironomus sp. 9 0 1 0 2 0 0 12 Glyptotendipes sp. 0 0 1 0 6 0 0 7 Harnischia (?) sp. 0 0 0 0 0 0 0 0 Microtendipes sp. (light form) 0 0 0 0 0 0 0 0 Parachironomus sp. (Larger; w/ enlarged shagreen mid-line T?) 36 0 5 0 5 0 0 46 Parachironomus sp. (Smaller; w/o enlarged shagreen middle T?) 0 0 4 0 0 0 0 4 Paracladopelma sp. 0 0 0 0 0 0 0 0 Paratendipes albimanus 0 0 0 0 0 0 0 0 Paratendipes sp. (small, spur pointed, genital sheaths elongated beyond AL) 0 0 Q 0 0 0 0 0 Phaenopsectra sp. 0 0 0 0 0 0 0 0 Polypedilum cf. brasinae 0 0 0 0 0 0 0 0 Polypedilum cf. illinoense 0 0 0 0 0 3 0 3 Polypedilum sp. 0 2 0 0 0 0 0 2 Polypedilum sp. 3 (large, w/o CT, corners of abd tergites darkened) 0 0 0 0 0 0 0 0 Polypedilum sp. (smaller, extensive shagreen, spines on T8 golden) 0 9 0 0 1 0 0 10 Pseudochironomus sp. 0 2 0 0 0 0 0 2 Robackia sp. 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 0 0 0 0 0 Stenochironomus sp. 0 0 0 0 0 0 0 0 Stictochironomus sp. 0 0 1 0 0 0 0 1 Zavreliella sp. 0 0 0 0 1 0 0 1 Genus near Gillotia/Parachironomus 0 0 0 0 0 0 0 0 Unknown Chironomini Genus 0 0 0 0 0 0 0 0 453 Tanytarsini Cladotanytarsus sp. 0 1 4 0 0 0 0 5 Micropsectra sp. (w/ short, weak spines T3) 0 0 0 0 0 3 0 3 Micropsectra sp. (w/ long, strong spines T3) 0 0 0 0 0 0 0 0 Micropsectra sp. 3 (spines only on T3, T4 & T5) 0 12 0 0 0 0 0 12 Paratanytarsus sp. (single patch & lateral spines T4, two patches T5) 2 0 0 0 0 0 0 2 Paratanytarsus sp. (single patch but no lateral spines T4, double patch T5) 34 7 11 1 0 1 0 54 Rheotanytarsus sp. (w/ paired patches only on T2-T4) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (paired patches T2-T5) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (w/ paired patches T2-T6) 0 0 0 0 0 0 0 ·0 Tanytarsus sp. (short spines, T3-T6) 0 62 0 0 5 2 0 69 Tanytarsus sp. (short spines, T2-T5?) 0 2 0 0 0 0 0 2 Tanytarsus sp. (short spines, T2-T6) 1 0 0 0 1 0 0 2 Tanytarsus sp. (long spines, T3-T4) 0 116 16 0 12 4 0 148 Tanytarsus sp. (overlapping spines like in Nimbocera) 0 0 5 0 2 0 0 7 Stempellina sp. 0 0 0 0 0 0 0 0 Stempellinella sp. 1 0 0 0 0 0 0 1 Tanypodinae Ablabesmyia sp. 3 0 0 0 3 0 0 6 Conchapelopia sp. (Very large plastron plate) 0 0 0 0 0 0 0 0 Conchapelopia sp. (Large plastron plate) 0 0 0 0 0 0 0 0 Conchapelopia sp. (Large plastron plate, LS T5-T8) 0 0 0 0 0 0 0 0 Guttipelopia sp. 0 2 0 0 0 0 0 2 Labrundinia sp. 0 0 0 0 0 0 0 0 Larsia sp. 0 2 0 0 0 0 0 2 Natarsia sp. 0 2 0 0 0 0 0 2 Nilotanypus sp. 0 0 0 0 0 0 0 0 Paramerina sp. 0 0 0 0 0 0 0 0 Procladius bellus 0 3 0 0 0 0 0 3 Procladius sublettei group 4 6 0 0 0 0 0 10 Psectrotanypus sp. 0 0 0 0 0 0 0 0 Tanypus sp. 0 1 0 0 0 0 0 1 Zavrelimyia sp. 0 0 0 0 0 0 0 0

Table 18. Cumulative taxa list fo r SFPE from site 1.5U identified under a dissection microscope. 454 Hardwood Creek: Site H-1 .4 (2004) Chironomidae SFPE identified with dissection microscope

Taxon 4/28 5/26 6/23 7/28 9/1 10/6 11/4 Total Diamesinae Diamesa sp. 0 0 0 0 0 0 0 0 Pagastia sp. 0 0 0 0 0 0 0 0 Prodiamesinae Odontomesa sp. 0 0 0 0 0 0 0 0 Prodiamesa sp. 0 0 0 0 0 0 0 0 Orthocladiinae Acricotopus sp. 2 0 1 0 1 4 0 8 Brillia sp. (small, light & w/ short RO) 0 0 0 0 0 0 0 0 Brillia sp. (large, dark & w/ long RO) 0 0 0 0 0 0 0 0 Cardiocladius sp. 0 0 0 0 0 0 0 0 Chaetocladius sp. (weak, elongate AMS) 0 0 0 0 0 0 0 0 Chaetocladius sp. (strong, short AMS) 0 0 0 0 0 0 0 0 Corynoneura sp. (full AFS) 17 0 1 0 0 4 0 22 Corynoneura sp. (AFS in distal one-half)) 0 0 0 0 0 0 0 0 Cricotopus (lsocladius) sp. 4 1 0 0 4 19 4 32 Cricotopus sp. (w/o RO) 0 0 0 0 0 0 0 0 Cricotopus sp. (w/ RO & PSB on T2) 0 0 0 0 0 17 0 17 Cricotopus sp. (w/ RO & PSB on T2 & T3) 0 1 0 2 0 1 11 15 Cricotopus sp. (large, clear sp. w/ large RO & PSB only on T2) 0 0 0 0 0 0 0 0 Cricotopus trifascia 0 0 0 0 0 0 0 0 Diplocladius cultriger 0 0 0 0 0 0 0 0 Doncricotopus sp. 0 0 0 0 0 0 0 0 Eukiefferiella sp. (darker species, w/ RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (lighter species w/ smaller RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (big, light golden & w/o RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (Large, dark gold/brown, tapering RO w/ drawn out tip) 0 0 0 0 0 0 0 0 Heleniella sp. 0 0 0 0 0 0 0 0 Heterotrissocladius sp. 0 0 0 0 0 0 0 0 Hudsonimyia sp. 0 0 0 0 0 0 0 0 455 Hydrobaenus sp. 0 0 0 0 0 0 0 0 Krenosmittia sp. 0 0 0 0 0 0 0 0 Limnophyes sp. 3 0 0 0 0 0 0 3 Lopescladius sp. 0 0 0 0 0 0 0 0 Metriocnemus sp. 0 0 0 0 0 0 0 0 Nanocladius sp. (digitiform RO) 1 0 2 0 0 1 0 4 Nanocladius sp. (pointed RO) 0 0 0 0 0 0 0 0 Nanocladius sp. (large bulbous RO w/ scales at tip) 0 0 0 0 0 0 0 0 Orthocladius (Eudactylocladius) sp. 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/ setal fringe) 0 0 0 0. 0 0 0 0 Orthocladius (Orthocladius) sp. (w/o setal fringe) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (PSB on T2 & T3) 0 . 0 0 0 0 0 0 0 Orthocladius (Orthocladius) cf. frigidus 0 0 0 0 0 0 0 0 Orthocladius (Symposiocladius) cf. lignicola 0 0 0 0 0 0 0 0 Parachaetocladius sp. 0 0 0 0 0 0 0 0 Paracricotopus sp. 0 0 0 0 0 0 0 0 Parakiefferiella sp 0 0 0 0 0 0 0 0 Parametriocnemus sp. (dark form) 0 0 0 0 0 0 0 0 Parametriocnemus sp. (light form) 0 0 0 0 0 0 0 0 Paraphaenocladius sp. 2 0 0 0 0 0 0 2 Psectrocladius sp. 0 0 0 0 0 0 0 0 Pseudosmittia sp. 1 0 0 1 0 0 0 2 Psilometriocnemus sp. 0 0 0 0 0 0 0 0 Rheocricotopus sp. 0 0 0 0 0 0 0 0 Rheosmittia sp. 0 0 0 0 0 0 0 0 Smittia sp. 0 0 0 0 0 0 0 0 Synorthocladius sp. 0 0 0 0 0 0 0 0 Thienemanniella sp. 0 1 6 0 0 1 0 8 Tvetenia sp. (dark form) 0 0 0 0 0 0 0 0 Tvetenia sp. (golden form) 0 0 0 0 0 0 0 0 Tvetenia sp. (very clear form) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus (near Stilocladius) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus #2 (Like Paraphaenocladius but w/ one AMS) 0 0 0 0 0 0 0 0 Chironomini Chironom�s sp. (w/o warts) 0 0 1 0 0 0 0 456 Chironomus sp. (w/ warts on frontal tubercles) 0 0 0 0 0 0 0 0 Cladopelma sp. 0 0 2 0 0 0 0 2 Crypto�hironomus (golden, large fused CT, bifid at very tip of CT) 0 0 0 0 0 0 0 0 Cryptochironomus (darker, CT rounded & bulbous at base w/ 2 spine-like branches) 0 0 0 0 0 0 0 0 Cryptochironomus (light golden, CT not fused at base) 0 0 0 0 0 0 0 0 Cryptochironomus (golden, CT fused & bulbous, tips like pronghorn) 0 0 0 0 0 0 0 0 Cryptochironomus (dark golden, CT not fused, very large sp.) 0 0 1 0 2 0 0 3 Cryptochironomus (darker brown, CT not fused at base) 0 0 0 0 0 0 0 0 Cryptotendipes sp. 0 0 0 0 0 0 0 0 Demeijeria sp. 0 0 1 0 0 0 0 1 Dicrotendipes fumidus 0 0 0 0 43 0 0 43 Dicrotendipes sp. (W/ sternal spines) 0 0 0 3 0 1 0 4 Endochironomus sp. 7 0 1 0 0 0 0 8 Glyptotendipes sp. 0 0 0 0 0 0 0 0 Harnischia (?) sp. 0 0 0 0 0 0 0 0 Microtendipes sp. (light form) 0 0 0 0 3 0 0 3 Parachironomus sp. (Larger; w/ enlarged shagreen midline T7) 1 0 2 0 0 1 0 4 Parachironomus sp. (Smaller; w/o enlarged shagreen middle T7) 0 0 0 0 0 0 0 0 Paracladopelma sp. 0 0 0 0 0 0 0 0 Paratendipes albimanus 0 0 0 0 5 0 0 5 Paratendipes sp. (small, spur pointed, genital sheaths elongated beyond AL) 0 0 0 0 0 0 0 0 Phaenopsectra sp. 0 0 0 0 0 0 0 0 Polypedilum cf. brasinae 0 0 0 0 0 0 0 0 Polypedilum cf. illinoense 0 0 0 0 1 0 0 1 Polypedilum sp. 0 0 0 0· 0 0 0 0 Polypedilum sp. 3 (large, w/o CT, corners of abd tergites darkened) 0 0 0 0 0 0 0 0 Polypedilum sp. (smaller, extensive shagreen, spines on T8 golden) 0 1 0 0 1 0 0 2 Pseudochironomus sp. 0 1 0 0 0 0 0 1 Robackia sp. 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 0 0 0 0 0 Stenochironomus sp. 0 0 0 0 0 0 0 0 Stictochironomus sp. 0 0 0 0 1 0 0 1 Zavreliella sp. 0 0 0 0 0 0 0 0 Genus near Gillotia/Parachironomus 0 0 0 0 0 0 0 0 Unknown Chironomini Genus 0 0 0 0 0 0 0 0 457 Tanytarsini • Cladotanytarsus sp. 0 0 0 0 0 0 0 0 Micropsectra sp. (w/ short, weak spines T3) 0 0 0 0 0 15 0 15 Micropsectra sp. (w/ long, strong spines T3) 0 0 0 0 0 2 0 2 Micropsectra sp. 3 (spines only on T3, T4 & T5) 0 1 0 0 0 0 0 1 Paratanytarsus sp. (single patch & lateral spines T4, two patches T5) 0 0 8 0 1 5 0 14 Paratanytarsus sp. (single patch but no lateral spines T4, double patch T5) 0 0 4 5 1 9 0 19 Rheotanytarsus sp. (w/ paired patches only on T2-T 4) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (paired patches T2-T5) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (w/ paired patches T2-T6) 0 0 0 0 0 0 0 0 Tanytarsus sp. (short spines, T3-T6) 0 0 0 1 0 8 0 9 Tanytarsus sp. (short spines, T2-T5?) 0 0 0 2 0 0 0 2 Tanytarsus sp. (short spines, T2-T6) 0 0 5 0 124 0 0 129 Tanytarsus sp. (long spines, T3-T4) 0 0 7 0 27 3 0 37 Tanytarsus sp. (overlapping spines like in Nimbocera) 14 0 3 0 36 2 0 55 Stempellina sp. 0 0 0 0 0 0 0 0 Stempellinella sp. 0 0 0 0 0 0 0 0 Tanypodinae Ablabesmyia sp. 1 0 0 0 9 0 0 10 Conchapelopia sp. (Very large plastron plate) 0 0 0 0 0 0 0 0 Conchapelopia sp. (Large plastron plate) 0 0 0 0 0 0 0 0 Conchapelopia sp. (Large plastron plate, LS T5-T8) 0 0 0 0 0 0 0 0 Guttipelopia sp. 0 0 0 0 0 0 0 0 Labrundinia sp. 0 0 0 0 0 0 0 0 Larsia sp. 0 0 0 0 0 1 0 1 Natarsia sp. 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 0 Paramerina sp. 0 0 0 0 0 0 0 0 Procladius bellus 0 1 0 1 0 0 0 2 Procladius sublettei group 1 0 0 2 7 0 0 10 Psectrotanypus sp. 0 0 0 0 0 0 0 0 Tanypus sp. 0 0 0 0 0 0 0 0 Zavrelimyia sp. 0 0 0 0 0 0 0 0

Table 19. Cumulative taxa list for SFPE from site 1.4 identified under a dissection microscope. 458 Hardwood Creek: Site H-1 .3 (2004) Chironomidae SFPE identified with dissection microscope

Taxon 4/28 5/26 6/23 7/28 9/1 10/6 11/4 Total Diamesinae Diamesa sp. 0 0 0 0 0 0 0 0 Pagastia sp. 0 0 0 0 0 0 0 0 Prodiamesinae Odontomesa sp. 0 0 0 0 0 0 0 0 Prodiamesa sp. 0 0 0 0 0 0 0 0 Orthocladiinae Acricotopus sp. 0 0 0 0 0 0 0 0 Brillia sp. (small, light & w/ short RO) 0 0 0 0 0 0 0 0 Brillia sp. (large, dark & w/ long RO) 0 0 0 0 0 0 0 0 Cardiocladius sp. 0 0 0 0 0 0 0 0 Chaetocladius sp. (weak, elongate AMS) 0 0 0 0 0 0 0 0 Chaetocladius sp. (strong, short AMS) 0 0 0 0 0 0 0 0 Corynoneura sp. (full AFS) 0 0 12 4 3 37 0 56 Corynoneura sp. (AFS in distal one-half)) 0 0 0 0 0 0 0 0 Cricotopus (lsocladius) sp. 11 2 0 0 0 36 2 51 Cricotopus sp. (w/o RO) 0 0 0 0 0 0 0 0 Cricotopus sp. (w/ RO & PSB on T2) 0 0 0 0 2 34 1 37 Cricotopus sp. (w/ RO & PSB on T2 & T3) 0 0 11 4 0 87 11 113 Cricotopus sp. (large, clear sp. w/ large RO & PSB only on T2) 0 0 0 0 0 0 0 0 Cricotopus trifascia 0 0 0 0 0 0 0 0 Diplocladius cultriger 0 0 0 0 0 0 0 0 Doncricotopus sp. 0 0 0 0 0 0 0 0 Eukiefferiella sp. (darker species, w/ RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (lighter species w/ smaller RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (big, light golden & w/o RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (Large, dark gold/brown, tapering RO w/ drawn out tip) 0 0 0 0 0 0 0 0 Heleniella sp. 0 0 0 0 0 0 0 0 Heterotrissocladius sp. 0 0 0 0 0 0 0 0 459 Hudsonimyia sp. 0 0 0 0 0 0 0 0 Hydrobaenus sp. 0 0 0 0 0 0 0 0 Krenosmittia sp. 0 0 0 0 0 0 0 0 Limnophyes sp. 1 0 0 0 0 0 0 1 Lopescladius sp. 0 0 0 0 0 0 0 0 Metriocnemus sp. 0 0 0 0 0 0 0 0 Nanocladius sp. (digitiform RO) 0 0 15 1 2 18 0 36 Nanocladius sp. (pointed RO) 0 0 3 0 0 0 0 3 Nanocladius sp. (large bulbous RO w/ scales at tip) 0 0 0 0 0 0 0 0 Orthocladius (Eudactylocladius) sp. 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/ setal fringe) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/o setal fringe) 6 0 0 0 0 0 0 6 Orthocladius (Orthocladius) sp. (PSB on T2 & T3) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) cf. frigidus 0 0 0 0 0 0 0 0 Orthocladius (Symposiocladius) cf. lignicola 0 0 0 0 0 0 0 0 Parachaetocladius sp. 0 0 0 0 0 0 0 0 Paracricotopus sp. 0 0 0 0 0 0 0 0 Parakiefferiella sp 0 0 0 0 0 2 0 2 Parametriocnemus sp. (dark form) 0 0 0 0 0 0 0 0 Parametriocnemus sp. (light form) 0 0 0 0 0 0 0 0 Paraphaenocladius sp. 0 0 0 0 0 0 0 0 Psectrocladius sp. 0 0 0 0 0 2 0 2 Pseudosmittia sp. 0 0 0 0 0 0 0 0 Psilometriocnemus sp. 0 0 0 0 0 0 0 0 Rheocricotopus sp. 0 0 0 0 0 0 0 0 Rheosmittia sp. 0 0 0 0 0 0 0 0 Smittia sp. 0 0 0 0 0 0 0 0 Synorthocladius sp. 0 0 0 0 0 0 0 0 Thienemanniella sp. 0 0 266 0 0 35 0 301 Tvetenia sp. (dark form) 0 0 0 0 0 0 0 0 Tvetenia sp. (golden form) 0 0 0 0 0 0 0 0 Tvetenia sp. (very clear form) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus (near Stilocladius) 0 0 0 0 0 0 0 0 460 Unknown Orthocladiinae Genus #2 (Like Paraphaenocladius but w/ one AMS) 0 0 0 0 0 0 0 0 Chironomini Chironomus sp. (w/o warts) 22 1 0 0 0 0 0 23 Chironomus sp. (w/ warts on frontal tubercles) 0 0 0 0 0 0 0 0 Cladopelma sp. 0 0 0 0 0 0 0 0 Cryptochironomus (golden, large fused CT, bifid at very tip of CT) 0 0 0 0 0 0 0 0 Cryptochironomus (darker, CT rounded & bulbous at base w/ 2 spine-like branches) 0 0 0 0 0 0 0 0 Cryptochironomus (light golden, CT not fused at base) 0 0 0 0 0 0 0 0 Cryptochironomus (golden, CT fused & bulbous, tips like pronghorn) 0 0 0 0 0 0 0 0 Cryptochironomus (dark golden, CT not fused, very large sp.) 0 0 0 0 1 0 0 1 Cryptochironomus (darker brown, CT not fu sed at base) 0 0 0 0 0 0 0 0 Cryptotendipes sp. 0 0 0 0 0 0 0 0 Demeijeria sp. 0 0 0 0 0 0 0 0 Dicrotendipes fumidus 0 0 0 0 0 0 0 0 Dicrotendipes sp. (W/ sternal spines) 0 0 0 0 0 0 0 0 Endochironomus sp. 1 0 0 0 0 0 0 1 Glyptotendipes sp. 0 0 0 0 0 0 0 0 Harnischia (?) sp. 0 0 0 0 0 0 0 0 Microtendipes sp. (light form) 0 0 0 0 0 0 0 0 Parachironomus sp. (Larger; w/ enlarged shagreen midline T7) 0 0 0 0 0 0 0 0 Parachironomus sp. (Smaller; w/o enlarged shagreen middle T7) 0 0 0 0 0 0 0 0 Paracladopelma sp. 0 0 0 0 0 0 0 0 Paratendipes albimanus 0 0 0 0 2 0 0 2 Paratendipes sp. (small, spur pointed, genital sheaths elongated beyond AL) 0 0 0 0 0 0 0 0 Phaenopsectra sp. 0 0 0 0 0 0 0 0 Polypedilum cf. brasinae 0 0 0 0 0 0 0 0 Polypedilum cf. illinoense 0 0 0 0 0 1 0 1 Polypedilum sp. 0 0 0 0 0 0 0 0 Polypedilum sp. 3 (large, w/o CT, corners of abd tergites darkened) 0 0 0 0 0 0 0 0 Polypedilum sp. (smaller, extensive shagreen, spines on T8 golden) 0 0 0 0 0 0 0 0 Pseudochironomus sp. 0 0 0 0 0 0 0 0 Robackia sp. 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 0 0 0 0 0 46 1 Stenochironomus sp. 0 0 0 0 0 0 0 0 Stictochironomus sp. 0 0 0 0 0 0 0 0 Zavreliella sp. 0 0 0 0 0 0 0 0 Genus near Gillotia/Parachironomus 0 0 0 0 0 0 0 0 Unknown Chironomini Genus 0 0 0 0 0 0 0 0 Tanytarsini Cladotanytarsus sp. 0 0 3 0 0 0 0 3 Micropsectra sp. (w/ short, weak spines T3) 0 0 0 0 0 6 0 6 Micropsectra sp. (w/ long, strong spines T3) 0 0 0 0 0 0 0 0 Micropsectra sp. 3 (spines only on T3, T4 & T5) 5 0 0 0 0 0 0 5 Paratanytarsus sp. (single patch & lateral spines T4, two patches T5) 2 0 0 0 1 17 0 20 Paratanytarsus sp. (single patch but no lateral spines T4, double patch T5) 0 0 0 0 0 41 0 41 Rheotanytarsus sp. (w/ paired patches only on T2-T 4) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (paired patches T2-T5) 0 0 0 a· 0 11 0 11 Rheotanytarsus sp. (w/ paired patches T2-T6) 0 0 0 0 0 0 0 0 Tanytarsus sp. (short spines, T3-T6) 0 0 0 0 0 2 0 2 Tanytarsus sp. (short spines, T2-T5?) 0 0 0 0 0 0 0 0 Tanytarsus sp. (short spines, T2-T6) 4 0 0 0 1 0 0 5 Tanytarsus sp. (long spines, T3-T4) 0 0 12 0 1 1 0 14 Tanytarsus sp. (overlapping spines like in Nimbocera) 0 0 0 0 1 1 0 2 Stempellina sp. 0 0 0 0 0 0 0 0 Stempellinella sp. 0 0 0 0 0 0 0 0 Tanypodinae Ablabesmyia sp. 0 0 0 0 3 0 0 3 Conchapelopia sp. (Very large plastron plate) 0 0 0 0 0 0 0 0 Conchapelopia sp. (Large plastron plate) 0 0 0 0 0 0 0 0 Conchapelopia sp. (Large plastron plate, LS T5-T8) 0 0 0 0 0 0 0 0 Guttipelopia sp. 0 0 0 0 0 0 0 0 Labrundinia sp. 0 0 0 0 0 0 0 0 Larsia sp. 0 0 0 0 1 0 0 1 Natarsia sp 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 0 Paramerina sp. 0 0 0 0 1 0 0 1 462 Procladius bellus 0 0 0 0 0 0 0 0 Procladius sublettei group 0 0 1 0 0 0 0 1 Psectrotanypus sp. 0 0 0 0 0 0 0 0 Tanypus sp. 0 0 0 0 0 0 0 0 Zavrelimyia sp. 0 0 0 0 0 0 0 0

Table 20. Cumulative taxa Hst for SFPE from site 1.3 identified under a dissection microscope.

Hardwood Creek: Site H-1 .50 (2004) Chironomidae SFPE identified with dissection microscope

Taxon 4/28 5/26 6/23 7/28 9/1 10/6 11/4 Total Diamesinae Diamesa sp. 0 0 0 0 0 0 0 0 Pagastia sp. 0 0 0 0 0 0 0 0 Prodiamesinae Odontomesa sp. 0 0 0 0 0 0 0 0 Prodiamesa sp. 0 0 0 0 0 0 0 0 Orthocladiinae Acricotopus sp. 0 0 0 0 0 0 0 0 Brillia sp. (small, light & w/ short RO) 0 0 0 0 0 0 0 0 Brillia sp. (large, dark & w/ long RO) 0 0 0 0 0 0 0 0 Cardiocladius sp. 0 0 0 0 0 0 0 0 Chaetocladius sp. (weak, elongate AMS) 0 0 0 0 0 0 0 0 Chaetocladius sp. (strong, short AMS) 0 0 0 0 0 0 0 0 Corynoneura sp. (full AFS) 0 10 1 0 2 0 0 13 Corynoneura sp. (AFS in distal one-half)) 0 0 0 0 0 0 0 0 Cricotopus (lsocladius) sp. 40 30 5 0 0 14 2 91 Cricotopus sp. (w/o RO) 0 0 0 0 0 0 0 0 Cricotopus sp. (w/ RO & PSB on T2) 0 0 0 0 0 12 1 13 Cricotopus sp. (w/ RO & PSB on T2 & T3) 0 15 0 0 0 0 4 19 Cricotopus sp. (large, clear sp. w/ large RO & PSB only on T2) 0 0 0 0 0 0 0 0 Cricotopus trifascia 0 0 0 0 0 0 0 0 Diplocladius cultriger 0 0 0 0 0 0 0 0 463 Doncricotopus sp. 0 0 0 0 0 0 0 0 Eukiefferiella sp. (darker species, w/ RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (lighter species w/ smaller RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (big, light golden & w/o RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (Large, dark gold/brown, tapering RO w/ drawn out tip) 0 0 0 0 0 0 0 0 Heleniella sp. 0 0 0 0 0 0 0 0 Heterotrissocladius sp. 0 0 0 0 0 0 0 0 Hudsonimyia sp. 0 0 0 0 0 0 0 0 Hydrobaenus sp. 0 0 0 0 0 0 0 0 Krenosmittia sp. 0 0 0 0 0 0 0 0 Limnophyes sp. 0 1 0 0 0 0 0 1 Lopescladius sp. 0 0 0 0 0 0 0 0 Metriocnemus sp. 0 0 0 0 0 0 0 0 Nanocladius sp. (digitiform RO) 0 14 3 0 1 0 0 18 Nanocladius sp. (pointed RO) 0 0 2 0 0 0 0 2 Nanocladius sp. (large bulbous RO w/ scales at tip) 0 0 0 0 0 0 0 0 Orthocladius (Eudactylocladius) sp. 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/ setal fringe) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/o setal fringe) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (PSB on T2 & T3) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) cf. frigidus 0 0 0 0 0 0 0 0 Orthocladius (Symposiocladius) cf. lignicola 0 0 0 0 0 0 0 0 Parachaetocladius sp. 0 0 0 0 0 0 0 0 Paracricotopus sp. 0 0 0 0 0 0 0 0 Parakiefferiella sp 0 0 0 0 0 0 0 0 Parametriocnemus sp. (dark form) 1 0 0 0 0 0 0 1 Parametriocnemus sp. (light form) 0 0 0 0 0 0 0 0 Paraphaenocladius sp. 0 0 0 0 0 0 0 0 Psectrocladius sp. 0 1 0 0 0 0 0 1 Pseudosmittia sp. 0 1 0 0 0 0 0 1 Psilometriocnemus sp. 0 0 0 0 0 0 0 0 Rheocricotopus sp. 0 0 0 0 0 0 0 0 Rheosmittia sp. 0 0 0 0 0 0 0 0 Smittia sp. 0 0 0 0 0 0 0 0 Synorthocladius sp. 0 0 0 0 0 0 0 0 464 Thienemanniella sp. 1 15 0 0 0 2 1 19 Tvetenia sp. (dark form) 0 0 0 0 0 0 0 0 Tvetenia sp. (golden form) 0 0 0 0 0 0 0 0 Tvetenia sp. (very clear form) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus (near Stilocladius) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus #2 (Like Paraphaenocladius but w/ one AMS) 0 0 0 0 0 0 0 0 Chironomini Chironomus sp. (w/o warts) 0 1 0 2 0 1 0 4 Chironomus sp. (w/ warts on frontal tubercles) 1 0 0 0 0 1 0 2 Cladopelma sp. 0 1 0 0 0 0 0 1 Cryptochironomus (golden, large fused CT, bifid at very tip of CT) 0 0 0 0 0 0 0 0 Cryptochironomus (darker, CT rounded & bulbous at base w/ 2 spine-like branches) 0 0 0 0 0 0 0 0 Cryptochironomus (light golden, CT not fused at base) 0 0 0 0 0 0 0 0 Cryptochironomus (golden, CT fused & bulbous, tips like pronghorn) 0 0 0 0 0 0 0 0 Cryptochironomus (dark golden, CT not fused, very large sp.) 0 0 1 0 0 0 0 1 Cryptochironomus (darker brown, CT not fused at base) 0 0 0 0 0 0 0 0 Cryptotendipes sp. 0 0 0 0 0 0 0 0 Demeijeria sp. 0 0 0 0 0 0 0 0 Dicrotendipes fu midus 0 0 0 0 0 0 0 0 Dicrotendipes sp. (with spines on tergites) 0 0 1 0 0 0 0 1 Endochironomus sp. 6 2 4 0 0 0 0 12 Glyptotendipes sp. 0 0 1 1 0 0 0 2 Harnischia (?) sp. 0 0 0 0 0 0 0 0 Microtendipes sp. (ligl:ltfo rm) 0 0 0 0 0 0 0 0 Parachironomus sp. (Larger; w/ enlarged shagreen mid-line T?) 5 0 296 0 0 0 0 301 Parachironomus sp. (Smaller; w/o enlarged shagreen middle T?) 0 0 6 0 0 0 0 6 Paracladopelma sp. 0 0 0 0 0 0 0 0 Paratendipes albimanus 0 0 1 0 0 0 0 1 Paratendipes sp. (small, spur pointed, genital sheaths elongated beyond AL) 0 0 0 0 0 0 0 0 Phaenopsectra sp. 0 0 0 0 0 0 0 0 Polypedilum cf. brasinae 0 0 0 0 0 0 0 0 Polypedilum cf. illinoense 0 2 0 0 0 1 0 3 Polypedilum sp. 0 0 0 0 0 0 0 0 Polypedilum sp. 3 (large, w/o CT, corners of abd tergites darkened) 0 0 0 0 0 0 0 0 Polypedilum sp. (smaller, extensive shagreen, spines on T8 golden) 0 0 2 0 0 0 0 2 465 Pseudochironomus sp. 0 1 0 0 0 0 0 1 Robackia sp. 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 0 0 0 0 0 Stenochironomus sp. 0 0 0 0 0 0 0 0 Stictochironomus sp. 0 0 0 0 0 0 0 0 Zavreliella sp. 0 0 0 0 0 0 0 0 Genus near Gillotia/Parachironomus 0 0 4 0 0 0 0 4 Unknown Chironomini Genus 0 0 0 0 0 0 0 0 Tanytarsini Cladotanytarsus sp. 0 0 1 0 0 0 0 1 Micropsectra sp. (w/ short, weak spines T3) 0 36 15 0 0 12 0 63 Micropsectra sp. (w/ long, strong spines T3) 0 2 0 0 0 1 0 3 Micropsectra sp. 3 (spines only on T3, T4 & T5) 0 0 0 0 0 0 0 0 Paratanytarsus sp. (single patch & lateral spines T4, two patches T5) 1 0 0 0 0 0 0 1 Paratanytarsus sp. (single patch but no lateral spines T4, double patch T5) 6 18 3 0 0 0 0 27 Rheotanytarsus sp. (w/ paired patches only on T2-T4) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (paired patches T2-T5) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (w/ paired patches T2-T6) 0 0 0 0 0 0 0 · 0 Tanytarsus sp. (short spines, T3-T6) 0 5 0 0 0 1 0 6 Tanytarsus sp. (short spines, T2-T5?) 0 0 0 0 0 0 0 0 Tanytarsus sp. (short spines, T2-T6) 0 0 0 0 0 0 0 0 Tanytarsus sp. (long spines, T3-T 4) 0 1 0 0 1 0 0 2 Tanytarsus sp. (overlapping spines like in Nimbocera) 0 0 1 0 2 0 0 3 Stempellina sp. 0 0 0 0 0 0 0 0 Stempellinella sp. 0 0 0 0 0 0 0 0 Tanypodinae Ablabesmyia sp. 1 0 0 0 4 0 0 5 Conchapelopia sp. (Very large plastron plate) 0 0 0 0 0 0 0 0 Conchapelopia sp. (Large plastron plate) 0 0 0 0 0 0 0 0 Conchapelopia sp. (Large plastron plate, LS T5-T8) 0 0 18 0 0 0 0 18 Guttipelopia sp. 0 0 0 0 0 0 0 0 Labrundinia sp. 0 0 0 0 0 0 0 0 Larsia sp. 0 0 0 0 0 0 0 0 Natarsia sp. 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 0 466 Paramerina sp. 0 0 0 0 0 0 0 0 Procladius bellus 0 1 0 0 0 0 0 1 Procladius sublettei group 0 0 2 0 0 0 0 2 Psectrotanypus sp. 0 0 0 o· 0 0 0 0 Tanypus sp. 0 0 0 0 0 0 0 0 Zavrelimyia sp. 3 0 0 0 0 0 0 3

Table 21. Cumulative taxa list fo r SFPE from site 1.50 identified under a dissection microscope.

Hardwood Creek: Site H-1 (2004) Chironomidae SFPE identified with dissection microscope

Taxon 4/28 5/26 6/23 7/28 9/1 10/6 11/13 Total Diamesinae Diamesa sp. 0 0 0 0 0 0 0 0 Pagastia sp. 0 0 0 0 0 0 0 0 Prodiamesinae Odontomesa sp. 0 0 0 0 0 0 0 0 Prodiamesa sp. 0 0 0 0 0 0 0 0 Orthocladiinae Acricotopus sp. 0 2 0 0 0 0 0 2 Brillia sp. (small, light & w/ short RO) 0 0 0 0 0 0 0 0 Brillia sp. (large, dark & w/ long RO) 0 0 0 0 0 0 0 0 Cardiocladius sp. 0 0 0 0 0 0 0 0 Chaetocladius sp. (weak, elongate AMS) 0 0 0 0 0 0 0 0 Chaetocladius sp. (strong, short AMS) 0 0 0 0 0 0 0 0 Corynoneura sp. (full AFS) 0 0 34 133 122 246 0 535 Corynoneura sp. (AFS in distal one-half)) 0 0 0 0 25 0 0 25 Cricotopus (lsocladius) sp. 0 0 2 0 0 2 0 4 Cricotopus sp. (w/o RO) 0 0 0 0 0 0 0 0 Cricotopus sp. (w/ RO & PSB on T2) 0 0 0 0 0 15 0 15 Cricotopus sp. (w/ RO & PSB on T2 & T3) 1 6 66 1 1 2 0 77 Cricotopus sp. (large, clear sp. w/ large RO & PSB only on T2) 0 0 0 0 0 0 0 0 Cricotopus trifascia 0 0 0 0 0 0 0 0 Diplocladius cultriger 0 0 0 0 0 0 0 0 467 Doncricotopus sp. 0 0 14 0 0 0 0 14 Eukiefferiella sp. (darker species, w/ RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (lighter species w/ smaller RO) 1 0 0 0 0 0 0 1 Eukiefferiella sp. (big, light golden & w/o RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (Large, dark gold/brown, tapering RO w/ drawn out tip) 0 0 0 0 0 0 0 0 Heleniella sp. 0 0 0 0 0 0 0 0 Heterotrissocladius sp. 0 0 0 0 0 0 0 0 Hudsonimyia sp. 0 0 0 0 0 0 0 0 Hydrobaenus sp. 0 0 0 0 0 0 0 0 Krenosmittia sp. 0 0 0 0 0 0 0 0 Limnophyes sp. 0 1 0 0 0 1 0 2 Lopescladius sp. 0 0 0 0 0 0 0 0 Metriocnemus sp. 0 0 0 0 0 0 0 0 Nanocladius sp. (digitiform RO) 0 11 40 2 8 13 0 74 Nanocladius sp. (pointed RO) 0 0 9 0 0 0 0 9 Nanocladius sp. (large bulbous RO w/ scales at tip) 0 1 2 0 1 0 0 4 Orthocladius (Eudactylocladius) sp. 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/ setal fringe) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/o setal fringe) 24 11 0 0 0 0 7 42 Orthocladius (Orthocladius) sp. (PSB on T2 & T3) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) cf. frigidus 0 0 0 0 0 0 0 0 Orthocladius (Symposiocladius) cf. lignicola 0 0 0 0 0 0 0 0 Parachaetocladius sp. 0 0 0 0 0 0 0 0 Paracricotopus sp. 0 0 0 0 0 0 0 0 Parakiefferiella sp 0 0 5 0 1 1 0 7 Parametriocnemus sp. (dark form) 0 3 1 0 0 0 0 4 Parametriocnemus sp. (light form) 0 0 0 0 0 0 0 0 Paraphaenocladius sp. 0 0 0 0 0 0 0 0 Psectrocladius sp. 0 0 0 0 0 0 0 0 Pseudosmittia sp. 0 0 0 0 0 0 0· 0 Psilometriocnemus sp. 0 0 0 0 0 0 0 0 Rheocricotopus sp. 0 0 1 0 0 0 0 1 Rheosmittia sp. 0 0 0 0 0 0 0 0 Smittia sp. 0 0 0 0 0 0 0 0 Synorthocladius sp. 0 0 0 0 0 0 0 0 468 Thienemanniella sp. 0 2 20 12 2 29 0 65 Tvetenia sp. (dark form) 0 0 0 0 0 1 0 1 Tvetenia sp. (golden form) 0 0 2 0 0 0 0 2 Tvetenia sp. (very clear form) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus (near Stilocladius) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus #2 (Like Paraphaenocladius but w/ one AMS) 0 0 0 0 0 0 0 0 Chironomini Chironomus sp. (w/o warts) 3 0 0 0 0 0 0 3 Chironomus sp. (w/ warts on frontal tubercles) 2 0 0 0 0 0 0 2 Cladopelma sp. 0 0 0 0 0 0 0 0 Crypto�hironomus (golden, large fused CT, bifid at very tip of CT) 0 0 0 0 0 0 0 0 Cryptochironomus (darker, CT rounded & bulbous at base w/ 2 spine-like branches) 0 0 0 0 0 0 0 0 Cryptochironomus (light golden, CT not fused at base) 0 0 0 0 0 0 0 0 Cryptochironomus (golden, CT fused & bulbous, tips like pronghorn) 0 0 0 0 1 0 0 1 Cryptochironomus (dark golden, CT not fused, very large sp.) 0 0 0 0 0 0 0 0 Cryptochironomus (darker brown, CT not fu sed at base) 0 0 0 0 12 0 0 12 Cryptotendipes sp. 0 0 0 0 0 0 0 0 Demeijeria sp. 0 0 0 0 0 0 0 0 Dicrotendipes fumidus 0 0 0 0 2 0 0 2 Dicrotendipes sp. (w/ sternal spines) 0 5 0 0 0 0 0 5 Endochironomus sp. 0 0 0 0 0 0 0 0 Glyptotendipes sp. 0 1 0 0 0 0 0 1 Harnischia (?) sp. 0 0 0 1 4 0 0 5 Microtendipes sp. (light form) 0 0 0 0 0 0 0 0 Parachironomus sp. Larger; w/ enlarged shagreen midline T?) 0 0 0 0 0 0 0 0 Parachironomus sp. (Smaller; w/o enlarged shagreen middle T?) 0 0 0 0 0 0 0 0 Paracladopelma sp. 0 0 0 0 1 0 0 1 Paratendipes albimanus 0 0 0 0 1 0 0 1 Paratendipes sp. (small, spur pointed, genital sheaths elongated beyond AL) 0 0 0 0 0 0 0 0 Phaenopsectra sp. 0 0 0 0 2 2 0 4 Polypedilum cf. brasinae 0 0 0 0 0 0 0 0 Polypedilum cf. illinoense 0 2 0 0 1 1 0 4 Polypedilum sp. 0 0 0 0· 0 0 0 0 Polypedilum sp. 3 (large, w/o CT, corners of abd terg ites darkened) 0 1 1 0 0 0 0 2 Polypedilum sp. (smaller, extensive shagreen, spines on T8 golden) 0 2 1 11 14 0 0 28 469 Pseudochironomus sp. 0 0 0 0 0 0 0 0 Robackia sp. 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 0 3 0 0 3 Stenochironomus sp. 0 1 4 14 2 0 0 21 Stictochironomus sp. 0 0 0 0 0 0 0 0 Zavreliella sp. 0 0 0 0 0 0 0 0 Genus near Gillotia/Parachironomus 0 0 2 0 0 0 0 2 Unknown Chironomini Genus 0 0 0 6 0 0 0 6 Tanytarsini Cladotanytarsus sp. 0 1 3 7 20 0 0 31 Micropsectra sp. (w/ short, weak spines T3) 0 3 0 0 0 4 0 7 Micropsectra sp. (w/ long, strong spines T3) 0 4 0 0 0 0 0 4 Micropsectra sp. 3 (spines only on T3, T4 & T5) 2 2 1 0 0 0 0 0 23 Paratanytarsus sp. (single patch & lateral spines T4, two patches T5) 10 0 2 0 5 0 0 17 Paratanytarsus sp. (single patch but no lateral spines T4, double patch T5) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (w/ paired patches only on T2-T4) 0 0 1 0 7 0 0 8 Rheotanytarsus sp. (paired patches T2-T5) 0 0 0 0 0 1 0 1 Rheotanytarsus sp. (w/ paired patches T2-T6) 0 0 1 0 3 0 0 4 Tanytarsus sp. (short spines, T3-T6) 0 0 0 4 0 1 0 5 Tanytarsus sp. (short spines, T2-T5?) 1 0 0 0 0 0 0 1 Tanytarsus sp. (short spines, T2-T6) 0 0 0 0 10 0 0 10 Tanytarsus sp. (long spines, T3-T4) 0 0 49 3 11 0 0 63 Tanytarsus sp. (overlapping spines like in Nimbocera) 0 0 0 3 21 1 0 25 Stempellina sp. 0 0 0 0 0 0 0 0 Stempellinella sp. 0 0 0 0 0 0 0 0 Tanypodinae Ablabesmyia sp. 0 0 3 7 17 0 0 27 Conchapelopia sp. (Very large plastron plate) 0 0 0 0 3 0 0 3 Conchapelopia sp. (Large plastron plate) 0 0 0 0 1 0 0 1 Conchapelopia sp. (Large plastron plate, LS T5-T8) 0 0 2 0 0 0 0 2 Guttipelopia sp. 0 0 0 0 0 0 0 0 Labrundinia sp. 0 0 0 1 15 0 0 16 Larsia sp. 0 0 0 0 0 3 0 3 Natarsia sp. 0 1 0 0 0 0 0 1 Nilotanypus sp. 0 0 0 0 3 0 0 3 470 Paramerina sp. 0 0 0 0 4 0 0 4 Procladius bellus 0 0 0 0 0 0 0 0 Procladius sublettei group 0 0 0 0 1 0 0 1 Psectrotanypus sp. 0 0 0 0 0 0 0 0 Tanypus sp. 0 0 0 0 0 0 0 0 Zavrelimyia sp. 0 0 0 0 0 0 0 0

Table 22. Cumulative taxa list for SFPE from site 1 identified under a dissection microscope.

Hardwood Creek: Site H-1 .1 (2004) Chironomidae SFPE identified with dissection microscope

Taxon 4/28 5/26 6/23 7128 9/1 10/6 11/13 Total Diamesinae Diamesa sp. 0 0 0 0 0 0 0 0 Pagastia sp. 0 0 0 0 0 0 0 0 Prodiamesinae Odontomesa sp. 0 0 0 0 0 0 0 0 Prodiamesa sp. 0 0 0 0 0 0 0 0 Orthocladiinae Acricotopus sp. 0 0 0 0 0 0 0 0 Brillia sp. (small, light & w/ short RO) 0 0 0 0 0 0 0 0 Brillia sp. (large, dark & w/ long RO) 0 0 0 0 0 0 0 0 Cardiocladius sp. 0 0 0 0 0 0 0 0 Chaetocladius sp. (weak, elongate AMS) 0 0 0 0 0 0 1 1 Chaetocladius sp. (strong, short AMS) 0 0 0 0 0 0 0 0 Corynoneura sp. (full AFS) 0 2 9 16 36 137 0 200 Corynoneura sp. (AFS in distal one-half)) 0 0 0 0 0 0 0 0 Cricotopus (lsocladius) sp. 0 18 0 0 0 0 0 18 Cricotopus sp. (w/o RO) 0 0 0 0 0 0 0 0 Cricotopus sp. (w/ RO & PSB on T2) 0 8 0 0 0 1 0 9 Cricotopus sp. (w/ RO & PSB on T2 & T3) 0 0 7 1 0 1 0 9 Cricotopus sp. (large, clear sp. w/ large RO & PSB only on T2) 0 0 0 0 0 0 0 0

471 Cricotopus trifascia 0 0 0 0 0 0 0 0 Diplocladius cultriger 0 0 0 0 0 0 0 0 Doncricotopus sp. 0 0 2 0 0 0 0 2 Eukiefferiella sp. (darker species, w/ RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (lighter species w/ smaller RO) 2 1 0 0 0 0 0 3 Eukiefferiella sp. (big, light golden & wlo RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (Large, dark gold/brown, tapering RO w/ drawn out tip) 0 0 0 0 0 0 0 0 Heleniella sp. 0 0 0 0 0 0 0 0 Heterotrissocladius sp. 0 0 0 0 0 0 0 0 Hudsonimyia sp. 0 0 0 0 0 0 0 0 Hydrobaenus sp. 0 0 0 0 0 0 0 0 Krenosmittia sp. 0 0 0 0 0 0 0 0 Limnophyes sp. 0 1 0 0 0 0 0 1 Lopescladius sp. 0 0 0 0 0 0 0 0 Metriocnemus sp. 0 0 0 0 0 0 0 0 Nanocladius sp. (digitiform RO) 0 13 10 2 7 1 0 33 Nanocladius sp. (pointed RO) 0 0 1 0 0 0 0 1 Nanocladius sp. (large bulbous RO w/ scales at tip) 0 0 0 0 0 0 0 0 Orthocladius (Eudactylocladius) sp. 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/ setal fringe) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/o setal fringe) 10 5 0 0 0 0 8 23 Orthocladius (Orthocladius) sp. (PSB on T2 & T3) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) cf. frigidus 0 0 0 0 0 0 0 0 Orthocladius (Symposiocladius) cf. lignicola 0 0 0 0 0 0 0 0 Parachaetocladius sp. 0 0 0 0 0 0 0 0 Paracricotopus sp. 0 0 0 0 0 0 0 0 Parakiefferiella sp 0 1 4 0 0 1 0 6 Parametriocnemus sp. (dark form) 0 4 1 0 2 0 0 7 Parametriocnemus sp. (light form) 0 0 0 0 0 0 0 0 Paraphaenocladius sp. 0 0 0 0 0 0 0 0 Psectrocladius sp. 0 0 0 0 0 0 0 0 Pseudosmittia sp. 0 0 0 0 0 0 0 0 Psilo metriocnemus sp. 0 0 0 0 0 0 0 0 472 Rheocricotopus sp. 0 0 1 0 7 0 0 8 Rheosmittia sp. 0 0 0 0 0 0 0 0 Smittia sp. 0 0 0 0 0 0 0 0 Synorthocladius sp. 0 0 0 0 0 0 0 0 ThienemannieHa sp. 0 6 2 3 1 13 0 25 Tvetenia sp. (dark form) 0 0 0 0 0 0 1 1 Tvetenia sp. (golden form) 0 0 5 0 0 0 2 7 Tvetenia sp. (very clear form) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus (near Stilocladius) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus #2 (Like Paraphaenocladius but w/ one AMS) 0 0 0 0 0 0 0 0 Chironomini Chironomus sp. (w/o warts) 3 0 0 0 0 0 0 3 Chironomus sp. (w/ warts on frontal tubercles) 1 0 0 0 0 0 0 1 Cladopelma sp. 0 0 0 0 1 0 0 1 Cryptochironomus (golden, large fused CT, bifid at very tip of CT) 0 0 0 4 0 0 0 4 Cryptochironomus (darker, CT rounded & bulbous at base w/ 2 spine-like 0 0 0 0 0 0 0 0 branches) Cryptochironomus (light golden, CT not fused at base) 0 0 0 0 0 0 0 0 Cryptochironomus (golden, CT fused & bulbous, tips like pronghorn) 0 0 0 0 0 0 0 0 Cryptochironomus (dark golden, CT not fused, very large sp.) 0 0 0 0 0 0 0 0 Cryptochironomus (darker brown, CT not fused at base) 0 0 0 1 12 0 0 13 Cryptotendipes sp. 0 0 0 0 0 0 0 0 Demejeiria sp. 0 0 0 0 0 0 0 0 Dicrotendipes fu midus 0 0 0 0 2 0 0 2 Dicrotendipes sp. (W/ sternal spines) 0 0 0 0 0 0 0 0 Endochironomus sp. 0 0 0 0 0 0 0 0 Glyptotendipes sp. 0 0 0 0 1 0 0 1 Harnischia (?) sp. 0 0 0 2 0 0 0 2 Microtendipes sp. (light form) 0 0 0 0 0 0 0 0 Parachironomus sp. (Larger; w/ enlarged shagreen midline T7) 0 0 0 0 0 0 0 0 Parachironomus sp. (Smaller; w/o enlarged shagreen middle T7) 0 0 0 0 0 0 0 0 Paracladopelma sp. 0 0 0 0 1 0 0 1 Paratendipes albimanus 0 0 0 0 0 0 0 0 473 Paratendipes sp. (small, spur pointed, genital sheaths elongated beyond AL) 0 0 0 0 2 0 0 2 Phaenopsectra sp. 0 0 2 1 2 0 0 5 Polypedilum cf. brasinae 0 0 0 0 5 0 0 5 Polypedilum cf. illinoense 0 0 0 1 15 7 0 23 Polypedilum sp. 0 0 0 0 1 0 0 1 Polypedilum sp. 3 (large, w/o CT, corners of abd tergites darkened) 0 0 0 0 0 0 0 0 Polypedilum sp. (smaller, extensive shagreen, spines on T8 golden) 0 1 2 12 4 0 0 19 Pseudochironomus sp. 0 0 0 0 0 0 0 0 Robackia sp. 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 0 3 2 0 0 5 Stenochironomus sp. 0 0 0 15 3 0 0 18 Stictochironomus sp. 0 0 0 0 0 0 0 0 Zavreliella sp. 0 0 0 0 0 0 0 0 Genus near Gillotia/Parachironomus 0 0 0 0 0 0 0 0 Unknown Chironomini Genus 0 0 0 6 0 0 0 6 Tanytarsini Cladotanytarsus sp. 0 0 7 12 15 0 0 34 Micropsectra sp. (w/ short, weak spines T3) 0 42 0 0 0 3 0 45 Micropsectra sp. (w/ long, strong spines T3) 0 1 0 0 0 0 0 1 Micropsectra sp. 3 (spines only on T3, T4 & T5) 0 0 0 0 0 0 0 0 Paratanytarsus sp. (single patch & lateral spines T4, two patches T5) 12 0 0 0 1 o· 0 13 Paratanytarsus sp. (single patch but no lateral spines T4, double patch T5) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (w/ paired patches only on T2-T 4) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (paired patches T2-T5) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (w/ paired patches T2-T6) 0 0 0 0 1 0 0 1 Tanytarsus sp. (short spines, T3-T6) 0 0 0 2 0 0 0 2 Tanytarsus sp. (short spines, T2-T5?) 0 0 0 2 0 0 0 2 Tanytarsus sp. (short spines, T2-T6) 0 0 0 0 6 0 0 6 Tanytarsus sp. (long spines, T3-T4) 0 0 31 0 5 0 0 36 Tanytarsus sp. (overlapping spines like in Nimbocera) 0 0 0 0 6 0 0 6 Stempellina sp. 0 0 0 0 0 0 0 0 Stempellinella sp. 0 0 0 0 0 0 0 0 Tanypodinae 474 Ablabesmyia sp. 0 0 0 0 5 0 0 5 Conchapelopia sp. (Very large plastron plate) 0 0 0 0 0 0 0 0 Conchapelopia sp. (Large plastron plate) 0 0 0 0 1 0 0 1 Conchapelopia sp. (Large plastron plate, LS T5-T8) 0 0 0 0 0 0 0 0 Guttipelopia sp. 0 0 0 0 0 0 0 0 Labrundinia sp. 0 0 0 0 6 0 0 6 Larsia sp. 0 0 0 0 1 4 0 5 Natarsia sp. 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 1 0 0 1 Paramerina sp. 0 0 0 0 1 0 0 1 Procladius bellus 0 0 0 0 0 0 0 0 Procladius sublettei group 2 0 0 2 5 0 0 9 Psectrotanypus sp. 0 0 0 0 0 0 0 0 Tanypus sp. 0 1 0 0 0 0 0 1 Zavrelimyia sp. 0 0 0 0 0 0 0 0

Table 23. Cumulative taxa list for SFPE from site 1.1 identified under a dissection microscope.

Hardwood Creek: Site H-1 .2 (2004) Chironomidae SFPE identified with dissection microscope

Taxon 4/28 5/26 6/23 7/28 9/1 10/6 11/1 3 Total Diamesinae Diamesa sp. 0 0 0 0 0 0 0 0 Pagastia sp. 0 0 0 0 0 0 0 0 Prodiamesinae Odontomesa sp. 0 0 0 0 0 0 0 0 Prodiamesa sp. 0 0 0 0 0 0 0 0 Orthocladiinae Acricotopus sp. 0 0 0 0 0 0 0 0 Brillia sp. (small, light & w/ short RO) 0 0 0 0 0 0 0 0 Brillia sp. (large, dark & w/ long RO) 0 0 0 0 0 0 0 0 Cardiocladius sp. 0 0 0 0 0 0 0 0 475 Chaetocladius sp. (weak, elongate AMS) 0 0 0 0 0 0 1 1 Chaetocladius sp. (strong, short AMS) 0 0 0 0 0 0 0 0 Corynoneura sp. (full AFS) 0 0 5 28 11 76 0 120 Corynoneura sp. (AFS in distal one-half)) 0 0 0 0 0 0 0 0 Cricotopus (lsocladius) sp. 0 1 0 0 0 2 0 3 Cricotopus sp. (w/o RO) 0 0 0 0 0 0 0 0 Cricotopus sp. (w/ RO & PSB on T2) 0 5 0 0 0 51 0 56 Cricotopus sp. (w/ RO & PSB on T2 & T3) 1 0 37 17 4 32 0 91 Cricotopus sp. (large, clear sp. w/ large RO & PSB only on T2) 0 0 0 0 0 0 0 0 Cricotopus trifascia 0 32 1 0 0 0 0 33 Diplocladius cultriger 0 0 0 0 0 0 0 0 Doncricotopus sp. 0 0 0 0 0 0 0 0 Eukiefferiella sp. (darker species, w/ RO) 0 1 0 0 0 0 0 1 Eukiefferiella sp. (lighter species w/ smaller RO) 467 61 0 0 0 0 0 528 Eukiefferiella sp. (big, light golden & w/o RO) 0 0 0 0 0 0 0 0 Eukieffe riella sp. (Large, dark gold/brown, tapering RO w/ drawn out tip) 0 0 0 0 0 0 0 0 Heleniella sp. 0 0 0 0 0 0 0 0 Heterotrissocladius sp. 0 0 0 0 0 0 0 0 Hudsonimyia sp. 0 0 0 0 0 0 0 0 Hydrobaenus sp. 1 0 0 0 0 0 0 1 Krenosmittia sp. 0 0 0 0 0 0 0 0 Limnophyes sp. 0 0 0 0 0 3 0 3 Lopescladius sp. 0 0 0 0 0 0 0 0 Metriocnemus sp. 0 0 0 0 0 0 0 0 Nanocladius sp. (digitiform RO) 0 1 6 7 3 19 0 36 Nanocladius sp. (pointed RO) 0 0 1 0 0 0 0 1 Nanocladius sp. (large bulbous RO w/ scales at tip) 0 0 3 1 0 0 0 4 Orthocladius (Eudactylocladius) sp. 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/ setal fringe) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/o setal fringe) 31 5 0 0 0 1 13 50 Orthocladius (Orthocladius) sp. (PSB on T2 & T3) 0 0 0 0 0 1 2 3 Orthocladius (Orthocladius) cf. frigidus 0 0 0 0 0 0 0 0 Orthocladius (Symposiocladius) cf. lignicola 0 0 0 0 0 0 0 0 476 Parachaetocladius sp. 0 0 0 0 0 0 0 0 Paracricotopus sp. 0 0 0 0 0 0 0 0 Parakiefferiella sp 0 0 0 0 0 6 0 6 Parametriocnemus sp. (dark form) 0 1 1 0 0 3 0 5 Parametriocnemus sp. (light form) 0 0 0 0 0 0 0 0 Paraphaenocladius sp. 0 0 0 0 0 1 0 1 Psectrocladius sp. 0 0 0 0 0 0 0 0 Pseudosmittia sp. 0 0 0 0 0 0 0 0 Psilometriocnemus sp. 0 0 0 0 0 0 0 0 Rheocricotopus sp. 0 0 0 16 4 0 0 20 Rheosmittia sp. 0 0 0 0 0 0 0 0 Smittia sp. 0 0 0 1 0 0 0 1 Synorthocladius sp. 0 0 0 0 0 0 0 0 Thienemanniella sp. 0 0 8 18 5 28 0 59 Tvetenia sp. (dark form) 0 0 0 0 0 0 1 1 Tvetenia sp. (golden form) 0 0 23 4 0 1 3 31 Tvetenia sp. (very clear form) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus (near Stilocladius) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus #2 (Like Paraphaenocladius but w/ one AMS) 0 0 0 0 0 0 0 0 Chironomini Chironomus sp. (w/o warts) 0 0 0 0 0 2 0 2 Chironomus sp. (w/ warts on frontal tubercles) 0 0 0 3 0 0 0 3 Cladopelma sp. 0 0 0 0 0 0 0 0 Cryptochironomus (golden, large fused CT, bifid at very tip of CT) 0 0 0 0 0 0 0 0 Cryptochironomus (darker, CT rounded & bulbous at base w/ 2 spine-like 0 0 0 0 0 0 0 0 branches) Cryptochironomus (light golden, CT not fused at base) 0 0 0 0 2 0 0 2 Cryptochironomus (golden, CT fused & bulbous, tips like pronghorn) 0 0 0 0 1 0 0 1 Cryptochironomus (dark golden, CT not fused, very large sp.) 0 0 0 0 0 0 0 0 Cryptochironomus (darker brown, CT not fused at base) 0 0 0 0 2 0 0 2 Cryptotendipes sp. 0 0 0 0 0 0 0 0 Demeijeria sp. 0 0 0 0 0 0 0 0 Dicrotendipes fumidus 0 0 0 0 0 0 0 0 477 Dicrotendipes sp. (W/ sternal spines) 0 0 0 0 0 0 0 0 Endochironomus sp. 0 0 0 0 0 0 0 0 Glyptotendipes sp. 0 0 0 0 0 0 0 0 Harnischia (?) sp. 0 0 0 1 0 0 0 1 Microtendipes sp. (light form) 0 0 0 0 0 0 0 0 Parachironomus sp. (Larger; w/ enlarged shagreen midline T7) 0 0 1 0 0 0 0 1 Parachironomus sp. (Smaller; w/o enlarged shagreen middle T7) 0 0 0 0 0 0 0 0 Paracladopelma sp. 0 0 0 0 0 0 0 0 Paratendipes albimanus 0 0 0 0 1 0 0 1 Paratendipes sp. (small, spur pointed, genital sheaths elongated beyond AL) 0 0 0 0 0 0 0 0 Phaenopsectra sp. 0 0 0 0 0 0 0 0 Polypedilum cf. brasinae 0 0 0 0 0 0 0 0 Polypedilum cf. illinoense 0 0 0 4 4 0 0 8 Polypedilum sp. 0 1 0 0 0 0 0 1 Polypedilum sp. 3 (large, w/o CT, corners of abd tergites darkened) 0 0 0 0 0 0 0 0 Polypedilum sp. (smaller, extensive shagreen, spines on T8 golden) 0 0 0 2 1 0 0 3 Pseudochironomus sp. 0 0 0 0 0 0 0 0 Robackia sp. 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 2 0 0 0 0 2 Stenochironomus sp. 0 0 0 1 0 0 0 1 Stictochironomus sp. 0 0 0 0 0 0 0 0 Zavreliella sp. 0 0 0 0 0 0 0 0 Genus near Gillotia/Parachironomus 0 0 0 0 0 0 0 0 Unknown Chironomini Genus 0 0 0 2 0 o· 0 2 Tanytarsini Cladotanytarsus sp. 0 2 28 32 23 0 0 85 Micropsectra sp. (w/ short, weak spines T3} 0 5 0 0 0 8 0 13 Micropsectra sp. (w/ long, strong spines T3} 0 1 0 0 0 6 0 7 Micropsectra sp. 3 (spines only on T3, T4 & T5} 1 0 0 0 0 0 0 1 Paratanytarsus sp. (single patch & lateral spines T4, two patches T5} 13 0 0 0 0 3 0 16 Paratanytarsus sp. (single patch but no lateral spines T4, double patch T5} 1 0 0 1 0 0 0 2 Rheotanytarsus sp. (w/ paired patches only on T2-T4) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (paired patches T2-T5} 0 0 0 0 1 0 0 1 478 Rheotanytarsus sp. (w/ paired patches T2-T6) 0 0 0 5 3 3 0 11 Tanytarsus sp. (short spines, T3-T6) 0 0 0 2 0 5 0 7 Tanytarsus sp. (short spines, T2-T5?) 0 0 0 0 0 0 0 0 Tanytarsus sp. (short spines, T2-T6) 0 2 1 0 1 0 0 4 Tanytarsus sp. (long spines, T3-T4) 0 0 6 2 4 4 0 16 Tanytarsus sp. (overlapping splnes like in Nimbocera) 0 0 0 0 0 0 0 0 Stempellina sp. 0 0 0 0 0 0 0 0 Stempellinella sp. 0 0 0 0 0 0 0 0 Tanypodinae Ablabesmyia sp. 0 0 0 1 0 1 0 2 Conchapelopia sp. (Very large plastron plate) 0 0 0 0 1 0 0 1 Conchapelopia sp. (Large plastron plate) 0 0 0 0 1 0 0 1 Conchapelopia sp. (Large plastron plate, LS T5-T8) 0 0 1 0 0 0 0 1 Guttipelopia sp. 0 0 0 0 0 0 0 0 Labrundinia sp. 0 0 0 0 0 0 0 0 Larsia sp. 0 0 0 0 0 0 0 0 Natarsia sp. 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 0 Paramerina sp. 0 0 0 0 1 0 0 1 Procladius bellus 0 0 0 0 0 0 0 0 Procladius sublettei group 0 0 0 5 1 1 0 7 Psectrotanypus sp. 0 0 0 0 0 0 0 0 Tanypus sp. 0 0 0 0 0 0 0 0 Zavrelimyia sp. 0 0 0 0 0 0 0 0

Table 24. Cumulative taxa list fo r SFPE from site 1.2 identified under a dissection microscope.

Hardwood Creek: Site H-2 (2004) Chironomidae SFPE identified with dissection microscope

Taxon 4/28 5/26 6/23 7128 9/1 10/6 11/13 Total Diamesinae Diamesa sp. 0 0 0 0 0 0 0 0 479 Pagastia sp. 0 0 0 0 0 0 0 0 Prodiamesinae Odontomesa sp. 0 0 0 0 0 0 0 0 Prodiamesa sp. 0 0 0 0 0 0 0 0 Orthocladiinae Acricotopus sp. 0 0 0 0 0 0 0 0 Brillia sp. (small, light & wl short RO) 0 0 0 0 0 0 0 0 Brillia sp. (large, dark & w/ long RO) 0 0 0 0 0 0 0 0 Cardiocladius sp. 0 0 0 0 0 0 0 0 Chaetocladius sp. (weak, elongate AMS) 0 0 0 0 0 0 1 1 Chaetocladius sp. (strong, short AMS) 0 0 0 0 0 0 0 0 Corynoneura sp. (full AFS) 0 0 23 2 5 128 0 158 Corynoneura sp. (AFS in distal one-half)) 0 0 0 0 1 0 0 1 Cricotopus (lsocladius) sp. 0 1 0 0 0 0 0 1 Cricotopus sp. (w/o RO) 0 0 0 0 0 0 0 0 Cricotopus sp. (w/ RO & PSB on T2) 0 0 0 0 1 17 0 18 Cricotopus sp. (w/ RO & PSB on T2 & T3) 1 0 1 1 0 1 0 4 Cricotopus sp. (large, clear sp. w/ large RO & PSB only on T2) 0 0 0 0 0 0 0 0 Cricotopus trifascia 0 4 0 0 0 0 0 4 Diplocladius cultriger 1 0 0 0 0 0 0 1 Doncricotopus sp. 0 0 0 0 0 0 0 0 Eukiefferiella sp. (darker species, w/ RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (lighter species w/ smaller RO) 6 6 0 0 0 0 0 12 Eukiefferiella sp. (big, light golden & wlo RO) 0 0 0 0 0 0 0 0 Eukiefferiella sp. (Large, dark gold/brown, tapering RO w/ drawn out tip) 0 0 0 0 0 0 0 0 Heleniella sp. 0 0 0 0 0 0 0 0 Heterotrissocladius sp. 0 0 0 0 0 0 0 0 Hudsonimyia sp. 0 0 0 0 0 0 0 0 Hydrobaenus sp. 0 0 0 0 0 0 0 0 Krenosmittia sp. 0 0 0 0 0 0 0 0 Limnophyes sp. 1 0 0 0 0 2 0 3 Lopescladius sp. 0 0 0 0 0 0 0 0 Metriocnemus sp. 0 0 0 0 0 0 0 0 480 Nanocladius sp. (digitiform RO) 0 19 1 3 0 4 0 27 Nanocladius sp. (pointed RO) 0 0 0 0 0 0 0 0 Nanocladius sp. (large bulbous RO w/ scales at tip) 0 0 1 0 0 0 0 1 Orthocladius (Eudactylocladius) sp. 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/ setal fringe) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) sp. (w/o setal fringe) 20 8 0 0 0 1 18 47 Orthocladius (Orthocladius) sp. (PSB on T2 & T3) 0 0 0 0 0 0 0 0 Orthocladius (Orthocladius) cf. frigidus 0 0 0 0 0 0 0 0 Orthocladius (Symposiocladius) cf. lignicola 0 0 0 0 0 0 0 0 Parachaetocladius sp. 0 0 0 0 0 0 0 0 Paracricotopus sp. 0 0 0 0 0 0 0 0 Parakiefferiella sp 1 11 0 0 0 15 0 27 Parametriocnemus sp. (dark form) 0 0 0 0 0 2 0 2 Parametriocnemus sp. (light form) 0 0 0 0 0 0 0 0 Paraphaenocladius sp. 0 1 0 0 0 0 0 1 Psectrocladius sp. 0 0 0 0 0 0 0 0 Pseudosmittia sp. 0 0 0 0 0 0 0 0 Psilometriocnemus sp. 0 0 0 0 0 0 0 0 Rheocricotopus sp. 0 0 1 0 0 0 0 1 Rheosmittia sp. 0 0 0 0 0 0 0 0 Smittia sp. 0 0 0 0 0 0 0 0 Synorthocladius sp. 0 0 0 0 0 0 0 0 Thienemanniella sp. 0 1 8 2 3 22 0 36 Tvetenia sp. (dark form) 0 0 0 0 0 0 0 0 Tvetenia sp. (golden form) 0 0 3 0 0 0 1 4 Tvetenia sp. (very clear form) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus (near Stilocladius) 0 0 0 0 0 0 0 0 Unknown Orthocladiinae Genus #2 (Like Paraphaenocladius but w/ one AMS) 0 0 0 0 0 0 0 0 Chironomini Chironomus sp. (w/o warts) 5 0 0 0 0 4 0 9 Chironomus sp. (w/ warts on frontal tubercles) 1 0 0 0 1 0 0 2 Cladopelma sp. 0 0 0 0 0 0 0 0 Cryptochironomus (golden, large fused CT, bifid at very tip of CT) 0 0 0 0 0 0 0 0 481 Cryptochironomus (darker, CT rounded & bulbous at base w/ 2 spine-like 0 0 0 0 0 0 0 0 branches) Cryptochironomus (light golden, CT not fused at base) 0 0 0 0 2 0 0 2 Cryptochironomus (golden, CT fused & bulbous, tips like pronghorn) 0 0 0 0 0 0 0 0 Cryptochironomus (dark golden, CT not fused, very large sp.) 0 0 0 0 0 0 0 0 Cryptochironomus (darker brown, CT not fused at base) 0 0 0 0 12 0 0 12 Cryptotendipes sp. 0 0 0 0 1 0 0 1 Demeijeria sp. 0 0 0 0 0 0 0 0 Dicrotendipes fumidus 0 0 0 0 0 0 0 0 Dicrotendipes sp. (w/ tergal spines) 0 0 0 0 0 0 0 0 Endochironomus sp. 0 3 0 0 0 0 0 3 Glyptotendipes sp. 0 0 0 0 0 0 0 0 Harnischia (?) sp. 0 0 1 2 2 0 0 5 Microtendipes sp. (light form) 0 0 0 0 0 0 0 0 Parachironomus sp. (Larger; w/ enlarged shagreen midlineT7) 0 0 0 0 0 0 0 0 Parachironomus sp. (Smaller; w/0 enlarged shagreen middle T7) 0 0 0 0 0 0 0 0 Paracladopelma sp. 0 0 0 0 1 0 0 1 Paratendipes albimanus 0 0 0 0 0 0 0 0 Paratendipes sp. (small, spur pointed, genital sheaths elongated beyond AL) 0 0 0 1 0 0 0 1 Phaenopsectra sp. 0 0 0 0 2 0 0 2 Polypedilum cf. brasinae 0 0 0 0 0 0 0 0 Polypedilum cf. illinoense 0 0 2 1 8 7 0 18 Polypedilum sp. 0 0 0 0 0 0 0 0 Polypedilum sp. 3 (large, w/o CT, corners of abd tergites darkened) 0 0 0 0 2 0 0 2 Polypedilum sp. (smaller, extensive shagreen, spines on T8 golden) 0 7 3 4 4 0 0 18 Pseudochironomus sp. 0 0 0 0 0 0 0 0 Robackia sp. 0 0 0 0 0 0 0 0 Saetheria sp. 0 0 2 0 16 0 0 18 Stenochironomus sp. 0 0 0 2 0 0 0 2 Stictochironomus sp. 0 0 0 0 0 0 0 0 Zavreliella sp. 0 0 0 0 0 0 0 0 Genus near Gillotia/Parachironomus 0 0 0 0 0 0 0 0 Unknown Chironomini Genus 0 0 0 0 0 0 0 0 482 Tanytarsini Cladotanytarsus sp. 0 3 0 2 27 0 0 32 Micropsectra sp. (w/ short, weak spines T3) 0 27 0 0 0 7 0 34 Micropsectra sp. (w/ long, strong spines T3) 0 2 0 0 0 1 0 3. Micropsectra sp. 3 (spines only on T3, T4 & T5) 1 0 0 0 0 0 0 1 Paratanytarsus sp. (single patch & lateral spines T4, two patches T5) 5 0 0 0 0 1 0 6 Paratanytarsus sp. (single patch but no lateral spines T4, double patch T5) 1 0 0 0 0 1 0 2 Rheotanytarsus sp. (w/ paired patches only on T2-T 4) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (paired patches T2-T5) 0 0 0 0 0 0 0 0 Rheotanytarsus sp. (w/ paired patches T2-T6) 0 0 0 0 1 0 0 1 Tanytarsus sp. (short spines, T3-T6) 0 0 0 0 0 5 0 5 Tanytarsus sp. (short spines, T2-T5?) 0 0 0 0 0 2 0 2 Tanytarsus sp. (short spines, T2-T6) 0 0 0 0 7 1 0 8 Tanytarsus sp. (long spines, T3-T4) 0 0 1 0 25 0 0 26 Tanytarsus sp. (overlapping spines like in Nimbocera) 0 0 0 0 7 0 0 7 Stempellina sp. 0 0 0 0 0 0 0 0 Stempellinella sp. 0 0 0 0 0 0 0 0 Tanypodinae Ablabesmyia sp. 0 0 0 0 0 0 0 0 Conchapelopia sp. (Very large plastron plate) 0 0 0 0 0 0 0 0 Conchapelopia sp. (Large plastron plate) 0 0 0 0 0 0 0 0 Conchapelopia sp. (Large plastron plate, LS T5-T8) 0 0 0 0 0 0 0 0 Guttipelopia sp. 0 0 0 0 0 0 0 0 Labrundinia sp. 0 0 0 0 2 1 0 3 Larsia sp. 0 0 0 0 0 1 0 1 Natarsia sp. 0 0 0 0 0 0 0 0 Nilotanypus sp. 0 0 0 0 0 0 0 0 Paramerina sp. 0 0 0 0 0 0 0 0 Procladius bellus 0 0 0 0 0 0 0 0 Procladius sublettei group 1 0 0 0 3 1 0 5 Psectrotanypus sp. 0 0 0 0 0 0 0 0 Tanypus sp. 0 0 0 0 0 0 0 0 Zavrelimyia sp. 0 0 0 0 0 0 0 0 483 Table 25. Cumulative taxa list for SFPE from site 2 identified under a dissection microscope.

Hardwood Creek: Site H-1 .5U (2004) Chironomidae SFPE slide mounted subsamples

Taxon 4/28 5/26 6/23 10/6 Total Orthocladiinae Acricotopus nitidellus 5 0 0 0 5 Corynoneura sp.1 1 0 0 0 1 Corynoneura sp.2 3 0 0 0 3 Cricotopus bicinctus 0 0 0 0 0 Cricotopus polaris 0 0 0 0 0 Cricotopus sylvestris 6 0 3 0 9 Cricotopus tibialis 0 0 0 0 0

• Cricotopus (lsocladius) sp.1 0 0 0 0 0 Cricotopus sp.5 0 0 0 0 0 Diplocladius sp. 0 0 0 0 0 Doncricotopus bicaudatus 0 0 0 0 0 Eukiefferiella sp.2 0 0 0 0 0 Limnophyes sp.3 5 0 0 0 5 Nanocladius crassicornus 0 0 0 0 0 Nanocladius distinctus 0 0 0 0 0 Nanocladius incomptus 0 0 0 0 0 Nanocladius rectinervis 6 0 0 0 6 Nanocladius spiniplenus 0 0 0 0 0 Orthocladius clarkei 0 0 0 0 0 Orthocladius nigritus 0 0 0 0 0 Orthocladius obumbratus 0 0 0 0 0 Orthocladius oliveri 0 0 0 0 0 Orthocladius trigonolabis 0 0 0 0 0 Parakiefferiella sp.? 0 0 0 0 0 Parametriocnemus sp.1 0 0 0 0 0 484 Parametriocnemus sp.4 0 0 0 0 0 Paraphaenoclad ius exagitans 2 0 0 0 2 Psectrocladius schlienzi type 1 0 0 0 1 Pseudosmittia sp.1 1 0 0 0 1 Pseudosmittia sp.2 4 0 0 0 4 Rheocricotopus (Psilocricotopus) sp.1 0 0 0 0 0 Rheosmittia sp. 0 0 0 0 0 Thienemanniella boltoni 2 0 0 0 2 Thienemanniella lobapodema 3 0 3 0 6 Thienemanniella similis 0 0 1 0 1 Thienemanniella sp.1 0 0 0 0 0 Tvetenia sp.1 (cf. paucunca) 0 0 0 0 0 Chironomini & Pseudochironomini Chironomus sp.1 0 0 5 0 5 Chironomus sp.2 0 0 0 0 0 Cladopelma sp.1 0 2 0 0 2 Cladopelma sp.2 0 3 0 0 3 Cryptochironomus eminentia 0 0 0 0 0 Cryptochironomus ponderosus 0 0 0 0 0 Cryptochironomus sp.1 (nr. conus) 0 0 0 0 0 Cryptochironomus sp.2 0 0 2 0 2 Cryptotendipes sp. 0 0 0 0 0 Demeijeria sp. 0 0 0 0 0 Dicrotendipes modestus/neomodestus 0 0 0 0 0 Dicrotendipes nervosus gr. 0 0 0 0 0 Dicrotendipes tritomus 0 0 1 0 1 Dicrotendipes sp.4 0 0 1 0 1 Dicrotendipes sp.6 0 0 0 0 0 Einfeldia sp. gr. D 0 0 1 0 1 Endochironomus nigricans 6 0 1 0 7 Glyptotendipes sp.1 0 0 1 0 1 Harnischia sp. 0 0 0 0 0 Microtendipes sp.3 0 0 0 0 0 485 Parachironomus arcuatus gr. 0 0 0 0 0 Parachironomus frequens gr. 0 0 0 0 0 Parachironomus sp. gr. C 0 0 1 0 1 Parachironomus varus gr. 5 0 3 0 8 Parachironomus vitiosus gr. 0 0 5 0 5 Paracladopelma nereis 0 0 0 0 0 Paralauterborniella nigrohalterale 0 0 0 0 0 · Paratendipes sp.1 0 0 0 0 0 Paratendipes (?) sp.2 0 1 0 0 1 Phaenopsectra sp.1 0 0 0 0 0 Phaenopsectra sp.2 0 0 0 0 0 Polypedilum (nr. falciforme) 0 0 0 0 0 Polypedilum fa llax 0 0 0 0 0 Polypedilum illinoense/angulum 0 0 0 0 0 Polypedilum obtusum 0 0 0 0 0 Polypedilum scalaenum gr. sp.1 0 0 0 0 0 Polypedilum scalaenum gr. sp.8 0 0 0 0 0 Polypedilum simulans 0 1 0 0 1 Polypedilum trigonus 0 1 0 0 1 Polypedilum sp.4 0 0 0 0 0 Pseudochironomus richardsoni 0 1 0 0 1 Saetheria tylus 0 0 0 0 0 Saetheria sp.1 0 0 0 0 0 Stenochironomus sp.3 0 0 0 0 0 Zavrelia sp. 1 0 0 0 1 Tanytarsini Cladotanytarsus sp.1 0 0 2 0 2 Cladotanytarsus sp.2 0 0 2 0 2 Micropsectra nigripila 0 5 0 0 5 Micropsectra polita 0 0 0 0 0 Paratanytarsus inopertus gr. sp.1 0 0 0 0 0 Paratanytarsus inopertus gr. sp.3 4 0 0 1 5 Paratanytarsus inopertus gr. sp.4 1 0 0 0 1 486 Paratanytarsus (cf. laccophilus) 5 0 0 0 5 Paratanytarsus laccophilus gr. sp.1 0 0 0 0 0 Paratanytarsus sp.2 5 0 5 0 10 Rheotanytarsus distinctissimus 0 0 0 0 0 Rheotanytarsus sp.1 0 0 0 0 0 Tanytarsus confusus 0 0 0 0 0 Tanytarsus lobiger 0 0 1 0 1 Tanytarsus neoflavellus 0 0 5 0 5 Tanytarsus sepp 0 0 0 0 0 Tanytarsus wirthi 0 3 1 0 4 Tanytarsus sp.1 (cf. gregarius gr.) 0 0 1 0 1 Tanytarsus sp.1 0 0 0 0 0 0 Tanytarsus sp.11 0 1 5 2 8 Tanytarsus sp.12 0 0 0 0 0 Tanytarsus sp.16 0 0 0 0 0 Tanytarsus sp.19 0 0 0 0 0 Tanytarsus sp.21 0 0 0 0 0 Tanytarsus sp.22 1 0 0 0 1 Tanytarsus sp.24 0 0 1 0 1 Tanypodinae Ablabesmyia mallochi 0 0 0 0 0 Ablabesmyia monilis 3 0 0 0 3 Conchapelopia dusena 0 0 0 0 0 Conchapelopia rurika 0 0 0 0 0 Labrundinia pilosella 0 0 0 0 0 Larsia sp. 0 0 0 0 0 Nilotanypus fimbriatus 0 0 0 0 0 Paramerina sp. 0 0 0 0 0 Procladius bellus 0 0 0 0 0 Procladius freemani 4 0 0 0 4 Procladius sublettei/denticulatus 0 0 3 0 3 Tanypus sp. 0 1 0 0 1 Zavrelimyia sp. 0 0 0 0 0 487 Table 26. Cumulative taxa list fo r SFPE from site 1.5U subsampled and identified under a compound microscope.

Hardwood Creek: Site H-1 .4 (2004) Chironomidae SFPE slide mounted subsamples

Taxon 5/26 6/23 7128 9/1 Total Orthocladiinae Acricotopus nitidellus 0 1 0 1 2 Corynoneura sp.1 0 0 0 0 0 Corynoneura sp.2 0 0 0 0 0 Cricotopus bicinctus 0 0 0 0 0 Cricotopus polaris 0 0 0 2 2 Cricotopus sylvestris 0 0 0 0 0 Cricotopus tibialis 0 0 0 1 1 Cricotopus (lsocladius) sp.1 0 0 0 0 0 Cricotopus sp.5 0 0 0 0 0 Diplocladius sp. 0 0 0 0 0 Doncricotopus bicaudatus 0 0 0 0 0 Eukiefferiella sp.2 0 0 0 0 0 Limnophyes sp.3 0 0 0 0 0 Nanocladius crassicornus 0 0 0 0 0 Nanocladius distinctus 0 0 0 0 0 Nanocladius incomptus 0 0 0 0 0 Nanocladius rectinervis 0 1 0 0 1 Nanocladius spiniplenus 0 0 0 0 0 Orthocladius clarkei 0 0 0 0 0 Orthocladius nigritus 0 0 0 0 0 Orthocladius obumbratus 0 0 0 0 0 Orthocladius oliveri 0 0 0 0 0 Orthocladius trigonolabis 0 0 0 0 0 Parakiefferiella sp. 7 0 0 0 0 0 Parametriocnemus sp.1 0 0 0 0 0 Parametriocnemus sp.4 0 0 0 0 0 488 Paraphaenocladius exagitans 0 0 0 0 0 Psectrocladius schlienzi type 0 0 0 0 0 Pseudosmittia sp.1 0 0 0 0 0 Pseudosmittia sp.2 0 0 0 0 0 Rheocricotopus (Psilocricotopus) sp.1 0 0 0 0 0 Rheosmittia sp. 0 0 0 0 0 Thienemanniella boltoni 0 0 0 0 0 Thienemanniella lobapodema 0 1 0 0 1 Thienemanniella similis 0 0 0 0 0 Thienemanniella sp.1 0 4 0 0 4 Tvetenia sp.1 (cf. paucunca) 0 0 0 0 0 Chironomini & Pseudochironomini Chironomus sp.1 0 1 0 0 1 Chironomus sp.2 0 0 0 0 0 Cladopelma sp.1 0 1 0 0 1 Cladopelma sp.2 0 1 0 0 1 Cryptochironomus eminentia 0 0 0 0 0 Cryptochironomus ponderosus 0 0 0 0 0 Cryptochironomus sp.1 (nr. conus) 0 1 0 0 1 Cryptochironomus sp.2 0 0 0 2 2 Cryptotendipes sp. 0 0 0 0 0 Demeijeria sp. 0 1 0 0 1 Dicrotendipes modestus/neomodestus 0 0 0 0 0 Dicrotendipes nervosus gr. 0 0 0 0 0 Dicrotendipes tritomus 0 0 0 0 0 Dicrotendipes sp.4 0 0 2 10 12 Dicrotendipes sp.6 0 0 0 0 0 Einfeldia sp. gr. D 0 0 0 1 1 Endochironomus nigricans 0 1 0 0 1 Glyptotendipes sp.1 0 0 0 0 0 Harnischia sp. 0 0 0 0 0 Microtendipes sp.3 0 0 0 2 2 Parachironomus arcuatus gr. 0 0 0 0 0 489 Parachironomus frequens gr. 0 0 0 0 0 Parachironomus sp. gr. C 0 2 0 0 2 Parachironomus varus gr. 0 0 0 0 0 Parachironomus vitiosus gr. 0 0 0 0 0 Paracladopelma nereis 0 0 0 0 0 Paralauterborniella nigrohalterale 0 0 0 0 0 Paratendipes sp.1 0 0 0 5 5 Paratendipes (?) sp.2 0 0 0 0 0 Phaenopsectra sp.1 0 0 0 0 0 Phaenopsectra sp.2 0 0 0 0 0 Polypedilum (nr. falciforme) 0 0 0 1 1 Polypedilum fa llax 0 0 0 0 0 Polypedilum illinoense/angulum 0 0 0 0 0 Polypedilum obtusum 0 0 0 0 0 Polypedilum scalaenum gr. sp.1 0 0 0 0 0 Polypedilum scalaenum gr. sp.8 0 0 0 0 0 Polypedilum simulans 0 0 0 0 0 Polypedilum trigonus 0 0 0 0 0 Polypedilum sp.4 0 0 0 1 1 Pseudochironomus richardsoni 1 0 0 0 1 Saetheria tylus 0 0 0 0 0 Saetheria sp.1 0 0 0 0 0 Stenochironomus sp.3 0 0 0 0 0 Zavrelia sp. 0 0 0 0 0 Tanytarsini Cladotanytarsus sp.1 0 0 0 0 0 Cladotanytarsus sp.2 0 0 0 0 0 Micropsectra nigripila 1 0 0 0 1 Micropsectra polita 0 4 0 0 4 Paratanytarsus inopertus gr. sp.1 0 1 0 0 1 Paratanytarsus inopertus gr. sp.3 0 0 0 1 1 Paratanytarsus inopertus gr. sp.4 0 0 0 0 0 Paratanytarsus (cf. laccophilus) 0 0 0 0 0 490 Paratanytarsus laccophilus gr. sp.1 0 0 0 1 1 Paratanytarsus sp.2 0 4 0 0 4 Rheotanytarsus distinctissimus 0 0 0 0 0 Rheotanytarsus sp.1 0 0 0 0 0 Tanytarsus confusus 0 0 0 0 0 Tanytarsus lobiger 0 0 0 0 0 Tanytarsus neoflavellus 0 1 0 9 10 Tanytarsus sepp 0 2 0 0 2 Tanytarsus wirthi 0 3 0 0 3 Tanytarsus sp.1 (cf. gregarius gr.) 0 1 0 6 7 Tanytarsus sp.1 0 0 7 0 0 7 Tanytarsus sp.11 0 1 0 6 7 Tanytarsus sp.12 0 0 0 0 0 Tanytarsus sp.16 0 0 0 0 0 Tanytarsus sp.19 0 0 0 4 4 Tanytarsus sp.21 0 0 0 0 0 Tanytarsus sp.22 0 0 0 0 0 Tanytarsus sp.24 0 0 0 2 2 Tanypodinae Ablabesmyia mallochi 0 0 0 0 0 Ablabesmyia monilis 0 0 0 5 5 Conchapelopia dusena 0 0 0 0 0 Conchapelopia rurika 0 0 0 0 0 Labrundinia pilosella 0 0 0 0 0 Larsia sp. 0 0 0 0 0 Nilotanypus fimbriatus 0 0 0 0 0 Paramerina sp. 0 0 0 0 0 Procladius bellus 0 0 0 1 1 Procladius freemani 0 0 0 2 2 Procladius sublettei/denticulatus 0 0 0 2 2 Tanypus sp. 0 0 0 0 0 Zavrelimyia sp. 0 0 0 0 0

491 Table 27. Cumulative taxa list for SFPE from site 1.4 subsampled and identified under a compound microscope.

Hardwood Creek: Site H-1 .3 (2004) Chironomidae SFPE slide mounted subsamples

Taxon 4/28 5/26 6/23 9/1 Total Orthocladiinae Acricotopus nitidellus 0 0 0 0 0 Corynoneura sp.1 0 0 0 3 3 Corynoneura sp.2 0 0 0 0 0 Cricotopus bicinctus 0 0 8 0 8 · Cricotopus polaris 0 0 0 0 0 Cricotopus sylvestris 2 0 0 0 2 Cricotopus tibialis 0 0 0 1 1 Cricotopus (lsocladius) sp.1 0 0 0 1 1 Cricotopus sp.5 0 0 1 0 1 Diplocladius sp. 0 0 0 0 0 Doncricotopus bicaudatus 0 1 2 0 3 Eukiefferiella sp.2 0 0 0 0 0 Limnophyes sp.3 0 0 0 0 0 Nanocladius crassicornus 0 0 0 0 0 Nanocladius distinctus 0 0 1 0 1 Nanocladius incomptus 0 0 0 0 0 Nanocladius rectinervis 0 0 7 2 9 Nanocladius spiniplenus 0 0 0 0 0 Orthocladius clarkei 5 0 0 0 5 Orthocladius nigritus 0 0 0 0 0 Orthocladius obumbratus 2 0 0 0 2 Orthocladius oliveri 1 0 0 0 1 Orthocladius trigonolabis 0 0 0 0 0 Parakiefferiella sp. 7 0 0 0 0 0 Parametriocnemus sp.1 0 0 0 0 0 Parametriocnemus sp.4 0 0 0 0 0 492 Paraphaenocladius exagitans 0 0 0 0 0 Psectrocladius schlienzi type 0 0 0 0 0 Pseudosmittia sp.1 0 0 0 0 0 Pseudosmittia sp.2 0 0 0 0 0 Rheocricotopus (Psilocricotopus) sp.1 0 0 0 0 0 Rheosmittia sp. 0 0 0 0 0 Thienemanniella boltoni 0 0 3 0 3 Thienemanniella lobapodema 0 0 1 0 1 Thienemanniella similis 0 0 1 0 1 Thienemanniella sp.1 0 0 5 0 5 Tvetenia sp.1 (cf. paucunca) 0 0 0 0 0 Chironomini & Pseudochironomini Chironomus sp.1 17 0 0 0 17 Chironomus sp.2 0 0 0 0 0 Cladopelma sp.1 0 0 0 0 0 Cladopelma sp.2 0 0 0 0 0 Cryptochironomus eminentia 0 0 0 0 0 Cryptochironomus ponderosus 0 0 0 0 0 Cryptochironomus sp.1 (nr. conus) 0 0 0 0 0 Cryptochironomus sp.2 0 0 0 1 1 Cryptotendipes sp. 0 0 0 0 0 Demeijeria sp. 0 0 0 0 0 Dicrotendipes modestus/neomodestus 0 0 0 0 0 Dicrotendipes nervosus gr. 0 0 0 0 0 Dicrotendipes tritomus 0 0 0 0 0

· Dicrotendipes sp.4 0 0 0 0 0 Dicrotendipes sp.6 0 0 0 0 0 Einfeldia sp. gr. D 0 0 0 0 0 Endochironomus nigricans 1 0 0 0 1 Glyptotendipes sp.1 0 0 0 0 0 Harnischia sp. 0 0 0 0 0 Microtendipes sp.3 0 0 0 0 0 Parachironomus arcuatus gr. 0 0 0 0 0 493 Parachironomus frequens gr. 0 0 0 0 0 Parachironomus sp. gr. C 0 0 0 0 0 Parachironomus varus gr. 0 0 0 0 0 Parachironomus vitiosus gr. 0 0 0 0 0 Paracladopelma nereis 0 0 0 0 0 Paralauterborniella nigrohalterale 0 0 0 0 0 Paratendipes sp.1 0 0 0 2 2 Paratendipes (?) sp.2 0 0 0 0 0 Phaenopsectra sp.1 0 0 0 0 0 Phaenopsectra sp.2 0 0 0 0 0 Polypedilum (nr. falciforme) 0 0 0 0 0 Polypedilum fallax 0 0 0 0 0 Polypedilum illinoense/angulum 0 0 0 0 0 Polypedilum obtusum 0 0 0 0 0 Polypedilum scalaenum gr. sp.1 0 0 0 0 0 Polypedilum scalaenum gr. sp.8 0 0 0 0 0 Polypedilum simulans 0 0 0 0 0 Polypedilum trigonus 0 0 0 0 0 Polypedilum sp.4 0 0 0 0 0 Pseudochironomus richardsoni 0 0 0 0 0 Saetheria tylus 0 0 0 0 0 Saetheria sp.1 0 0 0 0 0 Stenochironomus sp.3 0 0 0 0 0 Zavrelia sp. 0 0 0 0 0 Tanytarsini Cladotanytarsus sp.1 0 0 1 0 1 Cladotanytarsus sp.2 0 0 0 0 0 Micropsectra nigripila 5 1 0 0 6 Micropsectra polita 0 1 0 0 1 Paratanytarsus inopertus gr. sp.1 1 0 0 1 2 Paratanytarsus inopertus gr. sp.3 1 0 0 0 1 Paratanytarsus inopertus gr. sp.4 0 0 0 0 0 Paratanytarsus (cf. laccophilus) 0 0 0 0 0 494 Paratanytarsus laccophilus gr. sp.1 0 0 0 0 0 Paratanytarsus sp.2 0 0 0 0 0 Rheotanytarsus distinctissimus 0 0 0 0 0 Rheotanytarsus sp.1 0 0 0 0 0 Tanytarsus confusus 0 0 0 0 0 Tanytarsus lobiger 0 0 1 0 1 Tanytarsus neoflavellus 0 0 0 0 0 Tanytarsus sepp 0 0 0 1� 1 Tanytarsus wirthi 0 0 0 0 0 Tanytarsus sp.1 (cf. gregarius gr.) 0 0 0 0 0 Tanytarsus sp.1 0 0 0 5 0 5 Tanytarsus sp.11 0 0 0 0 0 Tanytarsus sp.12 0 0 0 0 0 Tanytarsus sp.16 0 0 0 0 0 Tanytarsus sp. 19 3 0 2 1 6 Tanytarsus sp.21 0 0 0 0 0 Tanytarsus sp.22 1 0 0 0 1 Tanytarsus sp.24 0 0 0 0 0 Tanypodinae Ablabesmyia mallochi 0 0 0 0 0 Ablabesmyia monilis 0 0 0 3 3 Conchapelopia dusena 0 0 0 0 0 Conchapelopia rurika 0 0 0 0 0 Labrundinia pilosella 0 0 0 0 0 Larsia sp. 0 0 0 1 1 Nilotanypus fimbriatus 0 0 0 0 0 Paramerina sp. 0 0 0 0 0 Procladius bellus 0 0 1 0 1 Procladius freemani 0 0 0 0 0 Procladius sublettei/denticulatus 0 0 0 0 0 Tanypus sp. 0 0 0 0 0 . Zavrelimyia sp. 0 0 0 0 0

495 Table 28. Cumulative taxa list fo r SFPE from site 1.3 subsampled and identified under a compound microscope.

Hardwood Creek: Site H-1 .50 (2004) Chironomidae SFPE slide mounted subsamples

Taxon 4/28 5/26 6/23 Total Orthocladiinae Acricotopus nitidellus 0 0 0 0 Corynoneura sp.1 0 0 0 0 Corynoneura sp.2 0 4 0 4 Cricotopus bicinctus 0 6 0 6 Cricotopus polaris 0 0 0 0 Cricotopus sylvestris 10 5 5 20 Cricotopus tibialis 0 0 0 0 Cricotopus (lsocladius) sp.1 0 0 0 0 Cricotopus sp.5 0 0 0 0 Diplocladius sp. 0 0 0 0 Doncricotopus bicaudatus 0 0 0 0 Eukiefferiella sp.2 0 0 0 0 Limnophyes sp.3 0 0 0 0 Nanocladius crassicornus 0 0 0 0 Nanocladius distinctus 0 0 4 4 Nanocladius incomptus 0 0 0 0 Nanocladius rectinervis 0 5 3 8 Nanocladius spiniplenus 0 0 0 0 Orthocladius clarkei 0 0 0 0 Orthocladius nigritus 0 0 0 0 Orthocladius obumbratus 0 0 0 0 Orthocladius oliveri 0 0 0 0 Orthocladius trigonolabis 0 0 0 0 Parakiefferiella sp. 7 0 0 0 0 Parametriocnemus sp.1 1 0 0 1 Parametriocnemus sp.4 0 0 0 0 496 Paraphaenocladius exagitans 0 0 0 0 Psectrocladius schlienzi type 0 0 0 0 Pseudosmittia sp.1 0 0 0 0 Pseudosmittia sp.2 0 1 0 1 Rheocricotopus (Psilocricotopus) sp.1 0 0 0 0 Rheosmittia sp. 0 0 0 0 Thienemanniella boltoni 2 2 0 4 Thienemanniella lobapodema 0 4 0 4 Thienemanniella similis 0 1 0 1 Thienemanniella sp.1 0 0 0 0 Tvetenia sp.1 (cf. paucunca) 0 0 0 0 Chironomini & Pseudochironomini Chironomus sp.1 0 0 0 0 Chironomus sp.2 1 0 0 1 Cladopelma sp.1 0 0 0 0 Cladopelma sp.2 0 0 0 0 Cryptochironomus eminentia 0 0 0 0 Cryptochironomus ponderosus 0 0 0 0 Cryptochironomus sp.1 (nr. conus) 0 0 1 1 Cryptochironomus sp.2 0 0 0 0 Cryptotendipes sp. 0 0 0 0 Demeijeria sp. 0 0 0 0 Dicrotendipes modestus/neomodestus 0 0 0 0 Dicrotendipes nervosus gr. 0 0 0 0 Dicrotendipes tritomus 0 0 1 1 Dicrotendipes sp.4 0 0 0 0 Dicrotendipes sp.6 0 0 0 0 Einfeldia sp. gr. D 0 0 0 0 Endochironomus nigricans 6 0 5 11 Glyptotendipes sp.1 0 0 1 1 Harnischia sp. 0 0 0 0 Microtendipes sp.3 0 0 0 0 Parachironomus arcuatus gr. 0 0 1 1 497 Parachironomus frequens gr. 0 0 7 7 Parachironomus sp. gr. C 0 0 11 11 Parachironomus varus gr. 2 0 4 6 Parachironomus vitiosus gr. 3 0 0 3 Paracladopelma nereis 0 0 0 0 Paralauterborniella nigrohalterale 0 0 0 0 Paratendipes sp.1 0 0 1 1 Paratendipes (?) sp.2 0 0 0 0 Phaenopsectra sp.1 0 0 0 0 Phaenopsectra sp.2 0 0 0 0 Polypedilum (nr. falciforme) 0 0 0 0 Polypedilum fa llax 0 0 0 0 Polypedilum illinoense/angulum 0 0 0 0 Polypedilum obtusum 0 0 0 0 Polypedilum scalaenum gr. sp.1 0 0 0 0 Polypediluni scalaenum gr. sp.8 0 0 0 0 Polypedilum simulans 0 0 0 0 Polypedilum trigonus 0 2 1 3 Polypedilum sp.4 0 0 0 0 Pseudochironomus richardsoni 0 0 0 0 Saetheria tylus 0 0 0 0 Saetheria sp.1 0 0 0 0 Stenochironomus sp.3 0 0 0 0 Zavrelia sp. 0 0 0 0 Tanytarsini Cladotanytarsus sp.1 0 0 1 1 Cladotanytarsus sp.2 0 0 0 0 Micropsectra nigripila 0 5 5 10 Micropsectra polita 0 2 5 7 Paratanytarsus inopertus gr. sp.1 0 0 0 0 Paratanytarsus inopertus gr. sp.3 1 0 0 1 Paratanytarsus inopertus gr. sp.4 0 0 0 0 Paratanytarsus (cf. laccophilus) 5 0 0 5 498 Paratanytarsus laccophilus gr. sp.1 0 0 1 1 Paratanytarsus sp.2 2 5 1 8 Rheotanytarsus distinctissimus 0 0 0 0 Rheotanytarsus sp.1 0 0 0 0 Tanytarsus confusus 0 0 0 0 Tanytarsus lobiger 0 0 0 0 Tanytarsus neoflavellus 0 0 1 1 Tanytarsus sepp 0 0 0 0 Tanytarsus wirthi 0 4 0 4 Tanytarsus sp.1 (cf. gregarius gr.) 0 0 0 0 Tanytarsus sp.1 0 0 0 0 0 Tanytarsus sp.11 0 1 0 1 Tanytarsus sp.12 0 0 0 0 Tanytarsus sp.16 0 0 0 0 Tanytarsus sp.19 0 0 0 0 Tanytarsus sp.21 0 0 0 0 Tanytarsus sp.22 0 0 0 0 Tanytarsus sp.24 0 2 0 2 Tanypodinae Ablabesmyia mallochi 0 0 0 0 Ablabesmyia monilis 1 0 1 2 Conchapelopia dusena 0 0 0 0 Conchapelopia rurika 0 0 10 10 Labrundinia pilosella 0 0 0 0 Larsia sp. 0 0 0 0 Nilotanypus fimbriatus 0 0 0 0 Paramerina sp. 0 0 0 0 Procladius bellus 0 0 0 0 Procladius freemani 0 1 0 1 Procladius sublettei/denticulatus 0 0 2 2 Tanypus sp. 0 0 0 0 Zavrelimyia sp. 3 0 0 3

499 Table 29. Cumulative taxa list for SFPE from site 1.50 subsampled and identified under a compound microscope.

Hardwood Creek: Site H-1 (2004) Chironomidae SFPE slide mounted subsamples

Taxon 4/28 7/28 9/1 Total Orthocladiinae Acricotopus nitidellus 0 0 0 0 Corynoneura sp.1 0 9 10 19 Corynoneura sp.2 0 0 0 0 Cricotopus bicinctus 0 0 0 0 Cricotopus polaris 0 0 0 0 Cricotopus sylvestris 0 0 0 0 Cricotopus tibialis 0 0 0 0 Cricotopus (lsocladius) sp.1 0 0 1 1 Cricotopus sp.5 0 0 0 0 Diplocladius sp. 0 0 0 0 Doncricotopus bicaudatus 0 0 0 0 Eukiefferiella sp.2 1 0 0 1 Limnophyes sp.3 0 0 0 0 Nanocladius crassicornus 0 0 0 0 Nanocladius distinctus 0 0 0 0 Nanocladius incomptus 0 0 0 0 Nanocladius rectinervis 0 2 4 6 Nanocladius spiniplenus 0 0 0 0 Orthocladius clarkei 4 0 0 4 Orthocladius nigritus 1 0 0 1 Orthocladius obumbr(jltus 4 0 0 4 Orthocladius oliveri 0 0 0 0 Orthocladius trigonolabis 0 1 0 1 Parakiefferiella sp. 7 0 0 0 0 Parametriocnemus sp.1 0 0 0 0 Parametriocnemus sp.4 0 0 0 0 500 Paraphaenocladius exagitans 0 0 0 0 Psectrocladius schlienzi type 0 0 0 0 Pseudosmittia sp.1 0 0 0 0 Pseudosmittia sp.2 0 0 0 0 Rheocricotopus (Psilocricotopus) sp.1 0 0 1 1 Rheosmittia sp. 0 0 1 1 Thienemanniella boltoni 0 0 0 0 Thienemanniella lobapodema 0 1 0 1 Thienemanniella similis 0 0 0 0 Thienemanniella sp.1 0 0 0 0 Tvetenia sp.1 (cf. paucunca) 0 0 0 0 Chironomini & Pseudochironomini Chironomus sp.1 4 0 0 4 Chironomus sp.2 1 0 0 1 Cladopelma sp.1 0 0 0 0 Cladopelma sp.2 0 0 0 0 Cryptochironomus eminentia 0 0 0 0 Cryptochironomus ponderosus 0 0 1 1 Cryptochironomus sp.1 (nr. conus) 0 0 8 8 Cryptochironomus sp.2 0 0 0 0 Cryptotendipes sp. 0 0 0 0 Demeijeria sp. 0 0 0 0 Dicrotendipes modestus/neomodestus 0 0 0 0 Dicrotendipes nervosusgr . 0 0 0 0 Dicrotendipes tritomus 0 0 0 0 Dicrotendipes sp.4 0 0 0 0 Dicrotendipes sp.6 0 0 1 1 Einfeldia sp. gr. D 0 0 0 0 Endochironomus nigricans 0 0 0 0 Glyptotendipes sp.1 0 0 0 0 Harnischia sp. 0 1 1 2 Microtendipes sp.3 0 0 0 0 Parachironomus arcuatus gr. 0 0 0 0 501 Parachironomus frequens gr. 0 0 0 0 Parachironomus sp. gr. C 0 0 0 0 Parachironomus varus gr. 0 0 0 0 Parachironomus vitiosus gr. 0 0 0 0 Paracladopelma nereis 0 5 1 6 Paralauterborniella nigrohalterale 0 0 4 4 Paratendipes sp.1 0 0 1 1 Paratendipes (?) sp.2 0 0 0 0 Phaenopsectra sp.1 0 0 0 0 Phaenopsectra sp.2 0 0 2 2 Polypedilum (nr. falciforme) 0 0 0 0 Polypedilum fallax 0 0 0 0 Polypedilum illinoense/angulum 0 0 0 0 Polypedilum obtusum 0 0 0 0 Polypedilum scalaenum gr. sp.1 0 5 5 10 Polypedilum scalaenum gr. sp.8 0 0 0 0 Polypedilum simulans 0 0 0 0 Polypedilum trigonus 0 0 0 0 Polypedilum sp.4 0 0 0 0 Pseudochironomus richardsoni 0 0 0 0 Saetheria tylus 0 0 3 3 Saetheria sp . 1 0 0 5 5 Stenochironomus sp.3 0 5 2 7 Zavrelia sp. 0 0 0 0 Tanytarsini Cladotanytarsus sp.1 0 5 1 6 Cladotanytarsus sp.2 0 0 1 1 Micropsectra nigripila 2 0 0 2 Micropsectra polita 0 0 0 0 Paratanytarsus inopertus gr. sp.1 5 0 5 10 Paratanytarsus inopertus gr. sp.3 0 0 0 0 Paratanytarsus inopertus gr. sp.4 0 0 0 0 Paratanytarsus ( cf. laccophilus) 0 0 0 0 502 Paratanytarsus laccophilus gr. sp.1 0 0 0 0 Paratanytarsus sp.2 0 0 0 0 Rheotanytarsus distinctissimus 0 0 5 5 Rheotanytarsus sp.1 0 0 5 5 "'fanytarsus confusus 0 0 1 1 Tanytarsus lobiger 0 2 4 6 Tanytarsus neoflavellus 0 0 0 0 Tanytarsus sepp 0 2 3 5 Tanytarsus wirthi 0 0 0 0 Tanytarsus sp.1 (ct. gregarius gr.) 0 0 0 0 Tanytarsus sp.1 0 0 1 2 3 Tanytarsus sp.11 0 0 0 0 Tanytarsus sp.12 0 2 0 2 Tanytarsus sp.16 0 0 1 1 Tanytarsus sp.19 0 0 1 1 Tanytarsus sp.21 0 1 3 4 Tanytarsus sp.22 1 0 2 3 Tanytarsus sp.24 0 0 0 0 Tanypodinae Ablabesmyia mallochi 0 5 8 13 Ablabesmyia monilis 0 2 6 8 Conchapelopia dusena 0 0 0 0 Conchapelopia rurika 0 0 4 4 Labrundinia pilosella 0 1 5 6 Larsia sp. 0 0 0 0 Nilotanypus fimbriatus 0 0 3 3 Paramerina sp. 0 0 3 3 Procladius bellus 0 0 0 0 Procladius freemani 0 0 0 0 Procladius sublettei/denticulatus 0 0 1 1 Tanypus sp. 0 0 0 0 Zavrelimyia sp. 0 0 0 0

503 Table 30. Cumulative taxa list for SFPE from site 1 subsampled and identified under a compound microscope.

Hardwood Creek: Site H-1 .1 (2004) Chironomidae SFPE slide mounted subsamples

Taxon 4/28 7/28 9/1 Total Orthocladiinae Acricotopus nitidellus 0 0 0 0 Corynoneura sp.1 0 5 9 14 Corynoneura sp.2 0 0 0 0 Cricotopus bicinctus 0 0 0 0 Cricotopus polaris 0 0 0 0 Cricotopus sylvestris 0 0 0 0 Cricotopus tibialis 0 0 0 0 Cricotopus (lsocladius) sp.1 0 0 0 0 Cricotopus sp.5 0 0 0 0 Diplocladius sp. 0 0 0 0 Doncricotopus bicaudatus 0 0 0 0 Eukiefferiella sp.2 2 0 0 2 Limnophyes sp.3 0 0 0 0 Nanoclad�us crassicornus 0 0 0 0 Nanocladius distinctus 0 0 0 0 Nanocladius incomptus 0 0 0 0 Nanocladius rectinervis 0 2 0 2 Nanocladius spiniplenus 0 0 0 0 Orthocladius clarkei 3 0 0 3 Orthocladius nigritus 0 0 0 0 Orthocladius obumbratus 2 0 0 2 Orthocladius oliveri 4 0 0 4 Orthocladius trigonolabis 0 0 0 0 Parakiefferiella sp. 7 0 0 0 0 Parametriocnemus sp.1 0 0 0 0 Parametriocnemus sp.4 0 0 2 2 504 Paraphaenocladius exagitans 0 0 0 0 Psectrocladius schlienzi type 0 0 0 0 Pseudosmittia sp.1 0 0 0 0 Pseudosmittia sp.2 0 0 0 0 Rheocricotopus (Psilocricotopus) sp.1 0 0 5 5 Rheosmittia sp. 0 0 0 0 Thienemanniella boltoni 0 0 0 0 Thienemanniella lobapodema 0 1 1 2 Thienemanniella similis 0 0 0 0 Thienemanniella sp.1 0 0 0 0 Tvetenia sp.1 (cf. paucunca) 0 0 0 0 Chironomini & Pseudochironomini Chironomus sp.1 1 0 0 1 Chironomus sp.2 3 0 0 3 Cladopelma sp.1 0 0 0 0 Cladopelma sp.2 0 0 0 0 Cryptochironomus eminentia 0 3 0 3 Cryptochironomus ponderosus 0 0 0 0 Cryptochironomus sp.1 (nr. conus) 0 2 6 8 Cryptochironomus sp.2 0 0 0 0 Cryptotendipes sp. 0 0 0 0 Demeijeria sp. 0 0 0 0 Dicrotendipes modestus/neomodestus 0 0 1 1 Dicrotendipes nervosus gr. 0 0 1 1 Dicrotendipes tritomus 0 0 0 0 Dicrotendipes sp.4 0 0 0 0 Dicrotendipes sp.6 0 0 0 0 Einfeldia sp. gr. D 0 0 0 0 Endochironomus nigricans 0 0 0 0 Glyptotendipes sp.1 0 0 1 1 Harnischia sp. 0 2 1 3 Microtendipes sp.3 0 0 0 0 Parachironomus arcuatus gr. 0 0 0 0 505 Parachironomus frequens gr. 0 0 0 0 Parachironomus sp. gr. C 0 0 0 0 Parachironomus varus gr. 0 0 0 0 Parachironomus vitiosus gr. 0 0 0 0 Paracladopelma nereis 0 6 2 8 Paralauterborniella nigrohalterale 0 1 4 5 Paratendipes sp.1 0 0 0 0 Paratendipes (?) sp.2 0 0 0 0 Phaenopsectra sp.1 0 0 0 0 Phaenopsectra sp.2 0 0 2 2 Polypedilum (nr. falciforme) 0 0 0 0 Polypedilum fa llax 0 0 1 1 Polypedilum illinoense/angulum 0 1 5 6 Polypedilum obtusum 0 0 0 0 Polypedilum scalaenum gr. sp.1 0 1 1 2 Polypedilum scalaenum gr. sp.8 0 5 0 5 Polypedilum simulans 0 0 0 0 Polypedilum trigonus 0 0 0 0 Polypedilum sp.4 0 0 0 0 Pseudochironomus richardsoni 0 0 0 0 Saetheria tylus 0 3 2 5 Saetheria sp.1 0 0 4 4 Stenochironomus sp.3 0 5 3 8 Zavrelia sp. 0 0 0 0 Tanytarsini Cladotanytarsus sp.1 0 5 5 10 Cladotanytarsus sp.2 0 0 0 0 Micropsectra nigripila 0 0 0 0 Micropsectra polita 0 0 0 0 Paratanytarsus inopertus gr. sp.1 5 0 1 6 Paratanytarsus inopertus gr. sp.3 0 0 0 0 Paratanytarsus inopertus gr. sp.4 0 0 0 0 Paratanytarsus ( cf. laccophilus) 0 0 0 0 506 Paratanytarsus laccophilus gr. sp.1 0 0 0 0 Paratanytarsus sp.2 0 0 0 0 Rheotanytarsus distinctissimus 0 0 0 0 Rheotanytarsus sp .1 0 0 1 1 Tanytarsus confusus 0 0 2 2 Tanytarsus lobiger 0 0 2 2 Tanytarsus neoflavellus 0 0 0 0 Tanytarsus sepp 0 0 3 3 Tanytarsus wirthi 0 0 0 0 Tanytarsus sp.1 (cf. gregarius gr.) 0 0 0 0 Tanytarsus sp.1 0 0 2 2 4 Tanytarsus sp.11 0 0 0 0 Tanytarsus sp.12 0 0 1 1 Tanytarsus sp.16 0 0 0 0 Tanytarsus sp.19 0 0 0 0 Tanytarsus sp.21 0 0 0 0 Tanytarsus sp.22 0 0 0 0 Tanytarsus sp.24 0 0 0 0 Tanypodinae Ablabesmyia mallochi 0 0 0 0 Ablabesmyia monilis 0 0 4 4 Conchapelopia dusena 0 0 0 0 Conchapelopia rurika 0 0 1 1 Labrund inia pilosella 0 0 6 6 Larsia sp. 0 0 0 0 Nilotanypus fimbriatus 0 0 1 1 Paramerina sp. 0 0 2 2 Procladius bellus 0 0 0 0 Procladius freemani 1 1 0 2 Procladius sublettei/denticulatus 0 1 5 6 Tanypus sp. 0 0 0 0 Zavrelimyia sp. 0 0 0 0

507 Table 31. Cumulative taxa list for SFPE from site 1.1 subsampled and identified under a co mpound microscope.

Hardwood Creek: Site H-1 .2 (2004) Chironomidae SFPE slide mounted subsamples

Taxon 4/28 7/28 9/1 Total Orthocladiinae Acricotopus nitidellus 0 0 0 0 Corynoneura sp.1 0 5 4 9 Corynoneura sp.2 0 0 0 0 Cricotopus bicinctus 0 1 4 5 Cricotopus polaris 0 0 0 0 Cricotopus sylvestris 0 0 0 0 Cricotopus tibialis 0 0 0 0 Cricotopus (lsocladius) sp.1 0 0 0 0 Cricotopus sp.5 0 4 1 5 Diplocladius sp. 0 0 0 0 Doncricotopus bicaudatus 0 0 0 0 Eukiefferiella sp.2 5 0 0 5 Limnophyes sp.3 0 0 0 0 Nanocladius crassicornus 0 2 0 2 Nanocladius distinctus 0 1 0 1 Nanocladius incomptus 0 0 0 0 Nanocladius rectinervis 0 2 1 3 Nanocladius spiniplenus 0 0 1 1 Orthocladius clarkei 6 0 0 6 Orthocladius nigritus 0 0 0 0 Orthocladius obumbratus 4 0 0 4 Orthocladius oliveri 2 0 0 2 Orthocladius trigonolabis 0 0 0 0 Parakiefferiella sp. 7 0 0 0 0 Parametriocnemus sp.1 0 0 0 0 Parametriocnemus sp.4 0 0 0 0 508 Paraphaenocladius exagitans 0 0 0 0 Psectrocladius schlienzi type 0 0 0 0 Pseudosmittia sp.1 0 0 0 0 Pseudosmittia sp.2 0 0 0 0 Rheocricotopus (Psilocricotopus) sp.1 0 5 5 10 Rheosmittia sp. 0 0 0 0 Thienemanniella boltoni 0 0 0 0 Thienemanniella lobapodema 0 0 0 0 Thienemanniella similis 0 1 0 1 Thienemanniella sp.1 0 9 5 14 Tvetenia sp.1 (cf. paucunca) 0 4 0 4 Chironomini & Pseudochironomini Chironomus sp.1 0 0 0 0 Chironomus sp.2 0 3 0 3 Cladopelma sp.1 0 0 0 0 Cladopelma sp.2 0 0 0 0 Cryptochironomus eminentia 0 0 1 1 Cryptochironomus ponderosus 0 0 0 0 Cryptochironomus sp.1 (nr. conus) 0 0 4 4 Cryptochironomus sp.2 0 0 0 0 Cryptotendipes sp. 0 0 0 0 Demeijeria sp. 0 0 0 0 Dicrotendipes modestus/neomodestus 0 0 0 0 Dicrotendipes nervosus gr. 0 0 0 0 Dicrotendipes tritomus 0 0 0 0 Dicrotendipes sp.4 0 0 0 0 Dicrotendipes sp.6 0 0 0 0 Einfeldia sp. gr. D 0 0 0 0 Endochironomus nigricans 0 0 0 0 Glyptotendipes sp.1 0 0 0 0 Harnischia sp. 0 1 0 1 Microtendipes sp.3 0 0 0 0 Parachironomus arcuatus gr. 0 0 0 0 509 Parachironomus frequens gr. 0 0 0 0 Parachironomus sp. gr. C 0 0 0 0 Parachironomus varus gr. 0 0 0 0 Parachironomus vitiosus gr. 0 0 0 0 Paracladopelma nereis 0 2 0 2 Paralauterborniella nigrohalterale 0 0 0 0 Paratendipes sp.1 0 0 1 1 Paratendipes (?) sp.2 0 0 0 0 Phaenopsectra sp.1 0 0 0 0 Phaenopsectra sp.2 0 0 0 0 Polypedilum (nr. falciforme) 0 0 0 0 Polypedilum fa llax 0 0 0 0 Polypedilum illinoense/angulum 0 2 2 4 Polypedilum obtusum 0 2 3 5 Polypedilum scalaenum gr. sp.1 0 0 0 0 Polypedilum scalaenum gr. sp.8 0 0 0 0 Polypedilum simulans 0 0 0 0 Polypedilum trigonus 0 0 0 0 Polypedilum sp.4 0 0 0 0 Pseudochironomus richardsoni 0 0 0 0 Saetheria tylus 0 0 0 0 Saetheria sp.1 0 0 0 0 Stenochironomus sp.3 0 1 0 1 Zavrelia sp. 0 0 0 0 Tanytarsini Cladotanytarsus sp.1 0 5 6 11 Cladotanytarsus sp.2 0 0 0 0 Micropsectra nigripila 1 0 0 1 Micropsectra polita 0 0 0 0 Paratanytarsus inopertus gr. sp.1 5 0 0 5 Paratanytarsus inopertus gr. sp.3 0 0 0 0 Paratanytarsus inopertus gr. sp.4 0 0 0 0 Paratanytarsus ( cf. laccophilus) 0 0 0 0 510 Paratanytarsus laccophilus gr. sp.1 0 0 0 0 Paratanytarsus sp.2 1 0 0 1 Rheotanytarsus distinctissimus 0 0 0 0 Rheotanytarsus sp.1 0 4 4 8 Tanytarsus confusus 0 0 0 0 Tanytarsus lobiger 0 1 0 1 Tanytarsus neoflavellus 0 0 0 0 Tanytarsus sepp 0 0 0 0 Tanytarsus wirthi 0 0 0 0 Tanytarsus sp.1 (cf. gregarius gr.) 0 0 1 1 Tanytarsus sp.1 0 0 4 2 6 Tanytarsus sp. 11 0 0 0 0 Tanytarsus sp.12 0 0 1 1 Tanytarsus sp .16 0 0 0 0 Tanytarsus sp.19 0 0 0 0 Tanytarsus sp.21 0 0 0 0 Tanytarsus sp.22 0 0 0 0 Tanytarsus sp.24 0 0 0 0 Tanypodinae Ablabesmyia mallochi 0 0 0 0 Ablabesmyia monilis 0 0 0 0 Conchapelopia dusena 0 0 1 1 Conchapelopia rurika 0 0 1 1 Labrundinia pilosella 0 0 0 0 Larsia sp. 0 0 0 0 Nilotanypus fimbriatus 0 0 0 0 Paramerina sp. 0 0 1 1 Procladius bellus 0 3 0 3 Procladius freemani 0 0 0 0 Procladius sublettei/denticulatus 0 2 2 4 Tanypus sp. 0 0 0 0 Zavrelimyia sp. 0 0 0 0

511 Table 32. Cumulative taxa list for SFPE from site 1.2 subsampled and identified under a compound microscope.

Hardwood Creek: Site H-2 (2004) Chironomidae SFPE slide mounted subsamples

Taxon 4/28 7128 9/1 Total Orthocladiinae Acricotopus nitidellus 0 0 0 0 Corynoneura sp.1 0 0 4 4 Corynoneura sp.2 0 0 0 0 Cricotopus bicinctus 0 0 0 0 Cricotopus polaris 0 0 0 0 Cricotopus sylvestris 0 0 0 0 Cricotopus tibialis 0 0 0 0 Cricotopus (lsocladius) sp.1 0 0 0 0 Cricotopus sp.5 0 0 1 1 Diplocladius sp. 1 0 0 1 Doncricotopus bicaudatus 0 0 0 0 Eukiefferiella sp.2 5 0 0 5 Limnophyes sp.3 1 0 0 1 Nanocladius crassicornus 0 0 0 0 Nanocladius distinctus 0 0 0 0 Nanocladius incomptus 0 1 0 1 Nanocladius rectinervis 0 0 0 0 Nanocladius spiniplenus 0 0 0 0 Orthocladius clarkei 5 0 0 5 Orthocladius nigritus 0 0 0 0 Orthocladius obumbratus 2 0 0 2 Orthocladius oliveri 3 0 0 3 Orthocladius trigonolabis 0 0 0 0 Parakiefferiella sp. 7 1 0 0 1 Parametriocnemus sp.1 0 0 0 0 Parametriocnemus sp.4 0 0 0 0 512 Paraphaenocladius exagitans 0 0 0 0 Psectrocladius schlienzi type 0 0 0 0 Pseudosmittia sp.1 0 0 0 0 Pseudosmittia sp.2 0 0 0 0 Rheocricotopus (Psilocricotopus) sp.1 0 2 0 2 Rheosmittia sp. 0 0 0 0 Thienemanniella boltoni 0 0 .0 0 Thienemanniella lobapodema 0 0 1 1 Thienemanniella similis 0 0 0 0 Thienemanniella sp.1 0 2 3 5 Tvetenia sp.1 (cf. paucunca) 0 0 0 0 Chironomini & Pseudochironomini Chironomus sp.1 4 0 1 5 Chironomus sp.2 1 0 0 1 Cladopelma sp.1 0 0 0 0 Cladopelma sp.2 0 0 0 0 Cryptochironomus eminentia 0 0 0 0 Cryptochironomus ponderosus 0 0 0 0 Cryptochironomus sp.1 (nr. conus) 0 0 8 8 Cryptochironomus sp.2 0 0 0 0 Cryptotendipes sp. 0 0 1 1 Demeijeria sp. 0 0 0 0 Dicrotendipes modestus/neomodestus 0 0 0 0 Dicrotendipes nervosus gr. 0 0 0 0 Dicrotendipes tritomus 0 0 0 0 Dicrotendipes sp.4 0 0 0 0 Dicrotendipes sp.6 0 0 0 0 Einfeldia sp. gr. D 0 0 0 0 Endochironomus nigricans 0 0 0 0 Glyptotendipes sp.1 0 0 0 0 Harnischia sp. 0 2 1 3 Microtendipes sp.3 0 0 0 0 Parachironomus arcuatus gr. 0 0 0 0 513 Parachironomus frequens gr. 0 0 0 0 Parachironomus sp. gr. C 0 0 0 0 Parachironomus varus gr. 0 0 0 0 Parachironomus vitiosus gr. 0 0 0 0 Paracladopelma nereis 0 0 1 1 Paralauterborniella nigrohalterale 0 0 2 2 Paratendipes sp.1 0 1 0 1 Paratendipes (?) sp.2 0 0 0 0 Phaenopsectra sp.1 0 0 1 1 Phaenopsectra sp.2 0 0 0 0 Polypedilum (nr. falciforme) 0 0 0 0 Polypedilum fallax 0 0 2 2 Polypedilum illinoense/angulum 0 0 4 4 Polypedilum obtusum 0 0 0 0 Polypedilum scalaenum gr. sp.1 0 1 1 2 Polypedilum scalaenum gr. sp.8 0 3 1 4 Polypedilum simulans 0 0 0 0 Polypedilum trigonus 0 0 0 0 Polypedilum sp.4 0 0 0 0 Pseudochironomus richardsoni 0 0 0 0 Saetheria tylus 0 0 5 5 Saetheria sp.1 0 0 0 0 Stenochironomus sp.3 0 2 0 2 Zavrelia sp. 0 0 0 0 Tanytarsini Cladotanytarsus sp.1 0 2 5 7 Cladotanytarsus sp.2 0 0 0 0 Micropsectra nigripila 1 0 0 1 Micropsectra polita 0 0 0 0 Paratanytarsus inopertus gr. sp.1 5 0 0 5 Paratanytarsus inopertus gr. sp.3 0 0 0 0 Paratanytarsus inopertus gr. sp.4 0 0 0 0 Paratanytarsus (cf. laccophilus) 0 0 0 0 514 Paratanytarsus laccophilus gr. sp.1 0 0 0 0 Paratanytarsus sp.2 1 0 0 1 Rheotanytarsus distinctissimus 0 0 0 0 Rheotanytarsus sp.1 0 0 1 1 Tanytarsus confusus 0 0 4 4 Tanytarsus lobiger 0 0 1 1 Tanytarsus neoflavellus 0 0 0 0 T anytarsus sepp 0 0 0 0 Tanytarsus wirthi 0 0 0 0 Tanytarsus sp.1 (cf. gregarius gr.) 0 0 5 5 Tanytarsus sp.1 0 0 0 5 5 Tanytarsus sp.11 0 0 0 0 Tanytarsus sp.12 0 0 0 0 Tanytarsus sp.16 0 0 0 0 Tanytarsus sp.19 0 0 0 0 Tanytarsus sp.21 0 0 0 0 Tanytarsus sp.22 0 0 0 0 Tanytarsus sp.24 0 0 0 0 Tanypodinae Ablabesmyia mallochi 0 0 0 0 Ablabesmyia monilis 0 0 0 0 Conchapelopia dusena 0 0 0 0 Conchapelopia rurika 0 0 0 0 Labrundinia pilosella 0 0 2 2 Larsia sp. 0 0 0 0 Nilotanypus fimbriatus 0 0 0 0 Paramerina sp. 0 0 0 0 Procladius bellus 0 0 0 0 Procladius freemani 1 0 3 4 Procladius sublettei/denticulatus 0 0 0 0 Tanypus sp. 0 0 0 0 Zavrelimyia sp. 0 0 0 0 !· 515

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