PPARGC1A Antibody Cat. No.: 7793

Western blot analysis of PPARGC1A in human heart tissue lysate with PPARGC1A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.

WB Validation in Human Lung Figure 3 WB Validation in Mouse Skin Loading: 15 μg of human lung lysate Antibodies: Loading: 15 μg of mouse skin lysate Antibodies: PPARGC1A 7793, 2 μg/mL , 1 h incubation at RT in 5% PPARGC1A 7793, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.

Figure 5 Immunofluorescence Validation of PPARGC1A Figure 4 WB Validation in Rat Kidney in MCF7 Cells Loading: 15 μg of rat kidney lysate Antibodies: PPARGC1A Immunofluorescent analysis of 4% paraformaldehyde- 7793, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. fixed MCF7 cells labeling PPARGC1A with 7793 at 20 Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 μg/mL, followed by goat anti-rabbit IgG secondary dilution. antibody at 1/500 dilution (green) and DAPI staining (blue).

September 30, 2021 1 https://www.prosci-inc.com/ppargc1a-antibody-7793.html Figure 7 Immunohistochemistry Validation of PPARGC1A in Human Colon Carcinoma Tissue Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PPARGC1A antibody (7793) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (94%)

PPARGC1A antibody was raised against a 17 peptide near the amino terminus of human PPARGC1A. IMMUNOGEN:

The immunogen is located within amino acids 200 - 250 of PPARGC1A.

TESTED APPLICATIONS: ELISA, IF, IHC-P, WB

WB: 2 μg/mL; IHC: 1-2 μg/mL; IF: 20 μg/mL.

APPLICATIONS: Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.

PPARGC1A antibody is human, mouse and rat reactive. At least three isoforms of SPECIFICITY: PPARGC1A are known to exist; this antibody will detect all three isoforms.

POSITIVE CONTROL: 1) Cat. No. 1419 - Mouse Skin Tissue Lysate

2) Cat. No. 1302 - Human Lung Tissue Lysate

3) Cat. No. 1224 - Daudi Cell Lysate

4) Cat. No. 1204 - K562 Cell Lysate

Predicted: 91kD PREDICTED MOLECULAR WEIGHT: Observed: 100 kD

September 30, 2021 2 https://www.prosci-inc.com/ppargc1a-antibody-7793.html Properties

PURIFICATION: PPARGC1A antibody is affinity chromatography purified via peptide column.

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PPARGC1A antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION: 1 mg/mL

PPARGC1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to STORAGE CONDITIONS: one year.

Additional Info

OFFICIAL SYMBOL: PPARGC1A

PPARGC1A Antibody: LEM6, PGC1, PGC1A, PGC-1v, PPARGC1, PGC-1(alpha), LEM6, ALTERNATE NAMES: Peroxisome proliferator-activated receptor gamma 1-alpha, Ligand effect modulator 6, PGC-1-alpha

ACCESSION NO.: NP_037393

PROTEIN GI NO.: 7019499

GENE ID: 10891

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A), also known as LEM-6, is a transcriptional coactivator that regulates the involved in energy (1). PPARGC1A interacts with PPARgamma, which permits the interaction of PPARGC1A with multiple transcription factors. PPARGC1A can interact with, BACKGROUND: and regulate the activities of, cAMP response element binding (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of , and is a major factor that regulates muscle fiber type determination (2). PPARGC1A may be also involved in the development of obesity (3).

1) Tsuemi T and La Spada AR. PGC-1a at the intersection of regulation and REFERENCES: neuron function: from Huntington’s disease to Parkinson’s disease and beyond. Prog. Neurobiol. 2012; 97:142-51.

2) Kang C and Li Ji L. Role of PGC-1a signaling in health and disease. Ann. NY Acad. Sci. 2012; 1271:110-7.

September 30, 2021 3 https://www.prosci-inc.com/ppargc1a-antibody-7793.html 3) Liu C and Lin JD. PGC-1 coactivators in the control of energy metabolism. Acta Biochim. Biophys. Sin. 2011; 43:248-57.

ANTIBODIES FOR RESEARCH USE ONLY.

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