Department of Govt. Holkar Science College, (M.P.) M.Sc. BIOCHEMISTRY (2017-2019) Scheme of Examination Course Duration- 2 years (4 Semesters)

Semester- I Code Theory/ Practical Teaching Credit Examination Scheme hours/ External Internal Total week Core 1 of Biomolecules 4 4 75 25 100 Core 2 Analytical Biochemistry 4 4 75 25 100 Core 3 Cell Biology 4 4 75 25 100 Core 4 Biostatistics 4 4 75 25 100 Practical Based on Theory paper 1 & 6 3 75 - 75 1 2 Practical Based on Theory paper 3 & 6 3 75 - 75 2 4 Seminar Seminar 1 1 1 - 25 25 Seminar 2/Field visit/ Ind. 1 1 - 25 25 visit 30 24 600 Board of Studies

S.No. Name Designation Signature 1. Prof. A.R. Batham Chairman 2. Dr. Purnima Dey Sarkar VC member 3. Prof. R.S. Gupta Subject Expert 4. Prof. R.S. Maheshwari Subject Expert 5. Mr. Rohan Gupta Representative from Industry 6. Dr. A. Bafna Member 7. Mrs. Tasneem Rangwala Member 8. Mr. Deepak Choudhary Member 9. Mrs Surya Sugandhi Member 10. Miss Pooja Gupta Alumini

Semester- II Code Theory/ Practical Teaching Credit Examination Scheme hours/ External Internal Total week Core 5 Physiology 4 4 75 25 100 Core 6 Microbial Biochemistry 4 4 75 25 100 Core 7 Nutritional Biochemistry 4 4 75 25 100 Core 8 Genetics 4 4 75 25 100 Practical Based on Theory paper 5 & 6 3 75 - 75 3 6 Practical Based on Theory paper 7 & 6 3 75 - 75 4 8 Seminar Seminar 3 1 1 - 25 25 Seminar 4/Field visit/ Ind. 1 1 - 25 25 visit 30 24 600

Board of Studies

S.No. Name Designation Signature 1. Prof. A.R. Batham Chairman 2. Dr. Purnima Dey Sarkar VC member 3. Prof. R.S. Gupta Subject Expert 4. Prof. R.S. Maheshwari Subject Expert 5. Mr. Rohan Gupta Representative from Industry 6. Dr. A. Bafna Member 7. Mrs. Tasneem Rangwala Member 8. Mr. Deepak Choudhary Member 9. Mrs Surya Sugandhi Member 10. Miss Pooja Gupta Alumini

Semester- III Code Theory/ Practical Teaching Credit Examination Scheme hours/ External Internal Total week Core 9 Enzymology 4 4 75 25 100 Core 10 Metabolism I 4 4 75 25 100 Elective 1 Plant Biochemistry or 4 4 75 25 100 Environmental Toxicology Elective 2 or 4 4 75 25 100 Advanced Biochemistry Open - 4 4 75 25 100 Elective I Interdisciplin ary Practical 5 Based on Theory paper 9 & 6 3 75 - 75 10 Practical 6 Based on Theory paper 1& 6 3 75 - 75 2 (elective) 32 26 650

Board of Studies

S.No. Name Designation Signature 1. Prof. A.R. Batham Chairman 2. Dr. Purnima Dey Sarkar VC member 3. Prof. R.S. Gupta Subject Expert 4. Prof. R.S. Maheshwari Subject Expert 5. Mr. Rohan Gupta Representative from Industry 6. Dr. A. Bafna Member 7. Mrs. Tasneem Rangwala Member 8. Mr. Deepak Choudhary Member 9. Mrs Surya Sugandhi Member 10. Miss Pooja Gupta Alumini Semester- IV Code Theory/ Practical Teaching Credit Examination Scheme hours/ External Internal Total week Core 11 Molecular Biology 4 4 75 25 100 Core 12 Clinical Biochemistry 4 4 75 25 100 Elective 3 Immunology Or 4 4 75 25 100 Computer application in Biochemistry Elective 4 Metabolism II Or Scientific Writing 4 4 75 25 100 Or Project Practical 7 Based on Theory paper 11 6 3 75 - 75 & 12 Practical 8 Based on Theory paper 3& 6 3 75 - 75 4 (elective) Internship 4 4 50 50 100 34 28 650 Total 100 100 2500 Board of Studies

S.No. Name Designation Signature 1. Prof. A.R. Batham Chairman 2. Dr. Purnima Dey Sarkar VC Member 3. Prof. R.S. Gupta Subject Expert 4. Prof. R.S. Maheshwari Subject Expert 5. Mr. Rohan Gupta Representative from Industry 6. Dr. A. Bafna Member 7. Mrs. Tasneem Rangwala Member 8. Mr. Deepak Choudhary Member 9. Mrs Surya Sugandhi Member 10. Miss Pooja Gupta Alumini

BIOCHEMISTRY M.Sc. SEMESTER I (2017-18) Paper 1: Chemistry of Biomolecules - 4 Credits UNIT – I

Carbohydrates: occurence, stereochemistry. Classification, structure, properties and biological importance of Carbohydrates. Isomerism in carbohydrates Mucopolysaccharide, Proteoglycans and amino sugars. Bacterial cell wall polysaccharide.

UNIT – II

Proteins: Classification, structure and properties of amino acids, essential amino acids, biologically active peptides, Classification and properties of proteins, sequencing of proteins, conformation and structure of proteins: primary, secondary, tertiary and quaternary structure, Forces stabilizing these structures, coagulation and denaturation of Proteins. Ramachandran Plot.

UNIT – III

Lipids: Definition, classification, structure and properties of fatty acid, Essential fatty acid. Chemical properties and characterization of fats. Waxes, cerebrosides, gangliosides. Phospholipids and proteolipids. Steroids and bile salts. Prostaglandins: structure properties and biological importance.

UNIT – IV

Nucleic acids: Structural constituents of nucleic acid- purines, pyrimidines, nucleosides and nucleotides, Biological role of nucleotides. Features of DNA double helix, Denaturation and annealing of DNA. Structure, types and biological role of RNA.

Prof. A.R. Batham Dr. A. Bafna Dr. Purnima Dey Sarkar Mrs. Tasneem Rangwala Prof. R.S. Gupta Mr. Deepak Choudhary Prof. R.S. Maheshwari Mrs Surya Sugandhi Mr. Rohan Gupta Miss Pooja Gupta

UNIT – V

Vitamins: Structure and biochemical properties of water soluble and fat soluble vitamins and their coenzyme activity.

Hormones: Mechanism of hormone action. Chemistry, role, hypo and hyperactivity of Insulin, Glucagon, Thyroxine, Cortisol,Growth Hormone, Estrogen, Progestrone and Testosterone.

REFERENCE BOOKS-

1. Text book of Biochemistry by West ,Todd 2. Biochemistry by A. Lehninger 3. Biochemistry by L. Stryer 4. Biochemistry by David Rawn 5. Harper’s Biochemistry

Prof. A.R. Batham Dr. A. Bafna Dr. Purnima Dey Sarkar Mrs. Tasneem Rangwala Prof. R.S. Gupta Mr. Deepak Choudhary Prof. R.S. Maheshwari Mrs Surya Sugandhi Mr. Rohan Gupta Miss Pooja Gupta

BIOCHEMISTRY

M.Sc. SEMESTER I (2017-18)

Paper 2: Analytical Biochemistry - 4 Credits

UNIT – I

The concept of pH, Dissociation and ionization of acids and bases, pKa, buffers and buffering mechanism, Henderson-Hasselbalch equation, ionization of amino acids and proteins, measurement of pH. General principle and different types of chromatography, adsorption and partition, Column, Paper and thin layer.

UNIT – II

Principle, materials used and applications of Ion-exchange chromatography, Gel filtration chromatography, Affinity chromatography and High Performance Liquid Chromatorgraphy.

UNIT – III

Electrophoresis: General Principle, factors affecting electrophoretic migration, Moving boundary and Zonal Electrophoresis, Paper and Gel Electrophoresis, PAGE and SDS PAGE, Isoelectric focussing technique.

Sedimentation: Basic principles of sedimentation, Sedimentation velocity, preparative and analytical ultracentrifugation techniques, differential and density gradient centrifugation, Subcellular fractionation.

UNIT-IV Radioactivity: Types of radioisotopes used in Biochemistry (32P, 35S, 14C and 3H) Disintegration of radionucleotides, half-life of radioactive compounds, measurement of radioactivity, scintillation counting, Use of radioisotopes in vivo and in vitro labelling, Isotopic tracer techniques, Autoradiography.

Prof. A.R. Batham Dr. A. Bafna Dr. Purnima Dey Sarkar Mrs. Tasneem Rangwala Prof. R.S. Gupta Mr. Deepak Choudhary Prof. R.S. Maheshwari Mrs Surya Sugandhi Mr. Rohan Gupta Miss Pooja Gupta UNIT – V

Spectrophotometry: Beer Lamberts law, extinction coefficient and its importance, design of colorimeter and spectrophotometer. Applications of UV-VIS spectrophotometry. Atomic absorption spectrophotometry and its applications in Biology.

Principle of Optical Rotatory Dispersion, Circular Dichroism and X-ray diffraction and their applications in structure determination. Principle of NMR spectroscopy. Application of NMR in Biology.

REFERENCE BOOKS-

1. Principles and techniques of Biochemistry by Wilson and Walker: 2. Biochemistry Laboratory: Modern Theory and Techniques by Rodney Boyer 3. Biophysical Chemistry (Principles and Techniques) by Upadhyay and Nath 4. Principles of Biochemistry: by A. Lehninger

Prof. A.R. Batham Dr. A. Bafna Dr. Purnima Dey Sarkar Mrs. Tasneem Rangwala Prof. R.S. Gupta Mr. Deepak Choudhary Prof. R.S. Maheshwari Mrs Surya Sugandhi Mr. Rohan Gupta Miss Pooja Gupta

BIOCHEMISTRY M.Sc. SEMESTER I (2017-18) Paper 3: Cell Biology - 4 Credits UNIT – I Cell- Difference between eukaryotic and prokaryotic cell, Structure of plant and animal cell, Plant cell wall and its composition, Plasmodesmata, Models of the biomembrane, structure, constituents and fluidity of plasma membrane, Cytoskeleton.

UNIT – II

Transport of metabolites across the Plasma membrane,Diffusion and Osmosis, non-mediated and mediated, passive and active transport. Primary and secondary active transport. Intracellular communication through gap junction, tight junction. Cell- cell interactions.

UNIT – III

Structure of Mitochondria, mitochondrial enzymes and their location, electron transport complexes, ATP synthase, mitochondrial DNA.

Structure of Chloroplast, protein complexes and photosynthetic electron transport chain, DNA of the Chloroplast.

UNIT – IV

Structure and functions of the Ribosomes. Structure, Types and Function of Endoplasmic Reticulum, protein sorting and signal hypothesis.

Prof. A.R. Batham Dr. A. Bafna Dr. Purnima Dey Sarkar Mrs. Tasneem Rangwala Prof. R.S. Gupta Mr. Deepak Choudhary Prof. R.S. Maheshwari Mrs Surya Sugandhi Mr. Rohan Gupta Miss Pooja Gupta

UNIT – V

Structure and function of Golgi body and Lysosomes, Mechanism of secretary processes. Structure of Nucleus: nuclear membrane and chromatin.

REFERENCE BOOKS-

1. Cell Biology by Karp 2. Cell Biology by De Roberti 3. The Cell: A Molecular Approach by Geoffrey M. Cooper 4. Cell Biology,Genetics, Molecular Biology: Evolution And Ecology by P.S.Verma and V.K Agrawal

Prof. A.R. Batham Dr. A. Bafna Dr. Purnima Dey Sarkar Mrs. Tasneem Rangwala Prof. R.S. Gupta Mr. Deepak Choudhary Prof. R.S. Maheshwari Mrs Surya Sugandhi Mr. Rohan Gupta Miss Pooja Gupta

BIOCHEMISTRY M.Sc. SEMESTER I (2017-18) Paper 4: Biostatistics - 4 Credit UNIT – I Types of data, Collection of data, Sampling and non-sampling methods.

Representation of Data: Frequency distribution. Line diagram, Bar diagram. Histogram and Relative Frequency, Histogram, Frequency Polygon and Frequency curve. Pie diagram, cumulative frequency distribution, Ogive and curve.

UNIT – II

Measures of Central Tendency: Arithmetic mean, Median, Mode, Geometric mean, Harmonic mean.

Measures of Dispersion: Range, Semi-interquartile range, Mean deviation, Standard deviation, Coefficient of variation, Skewness and their application in Biochemistry.

UNIT – III

Correlation and Regression: Scatter diagram. Correlation coefficient. Method of Least Squares. Fitting of regression line. Coefficient of determination. Non- Linear regression.

UNIT – IV

Probability and probability distributions: Classical and Statistical definitions of probability, Conditional Probability. Binomial, Poisson and Normal Distributions and their application in Biochemistry.

Prof. A.R. Batham Dr. A. Bafna Dr. Purnima Dey Sarkar Mrs. Tasneem Rangwala Prof. R.S. Gupta Mr. Deepak Choudhary Prof. R.S. Maheshwari Mrs Surya Sugandhi Mr. Rohan Gupta Miss Pooja Gupta

UNIT – V

Tests of Significance: Tests based on t, z, F and Chi-square distributions. Analysis of Variance: one way and two way classification and their applications in Biochemistry. p- value and its significance.

REFERENCE BOOKS-

1. Biostatistics by P.N. Arora ,P.K. Malhan 2. Biostatistics by R.Rama Krishanan 3. Biostatistics by B.N.Tondon 4. Statistics by D.N. Elhance 5. Biostatistics by B.K. Agrawal

Prof. A.R. Batham Dr. A. Bafna Dr. Purnima Dey Sarkar Mrs. Tasneem Rangwala Prof. R.S. Gupta Mr. Deepak Choudhary Prof. R.S. Maheshwari Mrs Surya Sugandhi Mr. Rohan Gupta Miss Pooja Gupta

M.Sc. Semester I Practical 1 Practical List

1. Qualitative tests of carbohydrates.

2. Qualitative tests of proteins.

3. Qualitative tests of lipids.

4. Detection of normal and abnormal constituents of urine.

5. Preparation of lecithin and cholesterol from egg yolk.

6. Separation of amino acids by chromatography

M.Sc. Semester I Practical 2 Practical List

1. Estimation of glucose by folin-wu method.

2. Estimation of glucose by anthrone method.

3. Estimation of protein by biuret method.

4. Determination of percent purity of Glycine by Sorensen’s formal titration.

5. Determination of iodine value of oils.

6. Determination of Saponification number of fats.

Prof. A.R. Batham Dr. A. Bafna Dr. Purnima Dey Sarkar Mrs. Tasneem Rangwala Prof. R.S. Gupta Mr. Deepak Choudhary Prof. R.S. Maheshwari Mrs Surya Sugandhi Mr. Rohan Gupta Miss Pooja Gupta