Ramaria Cistophila

334 Persoonia – Volume 39, 2017

Ramaria cistophila

b c Fungal Planet description sheets 335

Fungal Planet 651 – 20 December 2017 Ramaria cistophila P.P. Daniëls, M.P. Martín, C. Rojo & Camello, sp. nov.

Etymology. From Greek kistos (χιστός) and philos (φίλος), referring to its the eurosiberian region. Ramaria magnifica (= R. sardiniensis) host plants affinity, which are mainly Cistus species. differs by bearing a violet to purple hue on the base context and Classification — Gomphaceae, Gomphales, Phallomyceti- has a different ITS sequence; R. dolomitica also has a violet dae, Agaricomycetes. hue when bruised. The molecular analyses (parsimony and maximum likelihood), based on three collections of Ramaria Macroscopic characteristics — Basidiomata branched, initially cistophila, and a number of previously unpublished sequences obconical or obovoid, then campanulate or widely obovoid, 4–6 from our team, clearly grouped the new sequences with R. an- × 3–5.5 cm. Base glabrous, with basal branches 5–16 mm ziana (isotype) from Australia, collected under Nothofagus. thick, white; mycelium inconspicuous, white. Ramification poly- However, R. anziana has shorter spores (7–10.5 μm long ac- chotomous in the basal zone, otherwise, usually trichotomous, cording to Young 2014), and has a pinkish colour on branches. U- and V-shaped, ramification range 3–6; branches cylindri- Other closely related species is R. praecox from Europe but it cal, initially densely grouped, then divergent; orange yellow to is a vernal fruiting species with a different colour pattern, being orange with orange yellow spore print. Apices obtuse, short, completely yellow. initially yellow, then concolorous with the branches. Context fibrous, compact, brittle in the branches, homogeneous, concolorous with external surface; taste sour in branches, softer through the base; flavour which reminds one of a rubber eraser. Ramaria acrisiccescens AY102857 Chemical reactions: FSW (+) bluish green in hymenophore. Ramaria acrisiccescens AY102858 Microscopic characteristics — Generative hyphae without Ramaria cistophila, AH47765, MF564293*, Paratype 100/80 clamps, partially with ampulliform septa up to 15 µm diam, with Ramaria cistophila, AH47771, MF564294*, Paratype conspicuous granular ornamentation; hyphae of 4–9 µm wide Ramaria cistophila, AH47781, MF564292*, Holotype in the trama, with thin to moderately thick walls; hyphae of the Ramaria anziana TENN43401, mycelium 2–4(–5) µm wide, with thin to moderately thick walls; <50/66 MF564295*, Isotype Ramaria praecox, AH47730, MF564303* those of the external part sometimes with amorphous crystalline or granular incrustations. Secretory hyphae in the trama and Ramaria praecox, AH48383, MF564296* Ramaria praecox, Schild 2178, MF564297*, Isotype in the mycelium, the former linear and 2–4 µm wide, the latter 100/98 acanthodendroid 1.5–3 µm wide. Hyphidia filiform, 2–4 µm Ramaria praecox, AH47804, MF564298* wide. Basidia claviform, without basal clamp, (46–)50–73(–80) 99/84 Ramaria praecox, MA-Fungi 48102, MF564299* × (8.5–)9–11 µm, with 4 sterigmata. Spores oblong-elliptical, Ramaria admiratia KJ416133, Type (8.5–)9.5–11.5(–13) × 4–5(–5.5) µm (Lm = 10.9 µm; Wm = 4.7 100/100 Ramaria subtilis, AH48020, MF564300*

µm; Em = 2.3); spore wall thin, yellowish with verrucose orna- 68/<50 Ramaria subtilis, MA-Fungi 48010, MF564301* mentation. 100/100 Ramaria magniﬁca, MA-Fungi 26386, AJ408354 Ramaria magniﬁca, MA-Fungi 47982, AJ408355 Typus. Spain, Zamora, Mellanes, La Sierra, alt. 840 m, shrubland of Cistus ladanifer, 19 Nov. 2015, C. Rojo, AH 47781 (holotype, Herbarium of Ramaria boreimaxima KJ464996, Type Alcalá de Henares University; isotype, MA-Fungi 90716, Herbarium of the 89/68 Ramaria calvodistalis KJ416132, Type Real Jardín Botánico-CSIC, Madrid; ITS sequence GenBank MF564292, Ramaria foetida JQ408239 MycoBank MB822118). 100/98 Ramaria aurantiisiccescens EU837199 Additional materials examined. Spain, Cáceres, Aliseda, alt. 450 m, shrub- Ramaria aurantiisiccescens EU837198 land of Cistus ladanifer, 24 Jan. 2015, F. Camello, AH 47771, ITS and LSU sequences GenBank MF564294 and MF564304; Cádiz, Jimena de la Fron- Ramaria aurantiisiccescens JX310388 tera, Las Casillas, under Quercus suber, 29 Nov. 2014, A. Lobo, AH 47764; 87/74 Ramaria aurantiisiccescens EU837197 Cádiz, Jimena de la Frontera, Los Gavilanes, under Quercus suber and Cis- Ramaria aurantiisiccescens EU644709 tus, 29 Nov. 2014, L. Estrada, AH 47765, ITS sequence GenBank MF564293; Ramaria sanguinea, MA-Fungi 48080, AJ408373 Zamora, Mellanes, La Sierra, alt. 840 m, shrubland of Cistus ladanifer, 2 Nov. 100/100 2015, C. Rojo, AH 47782. Ramaria rubiginosa DQ365650 Ramaria largentii, AH48019, MF564302*

Notes — Although the appearance and micromorphology Ramaria aurea MA-Fungi 48120, AJ408387 looks like a small-sized basidiome of Ramaria aurea, R. cisto 0.05 substitutions/site phila has a dark green positive reaction with FSW, it grows The single maximum likelihood tree obtained through heuristic in a Mediterranean climate with Cistus shrubs and Quercus search with the model GTR + I + G selected in PAUP v. 4.0b10. suber trees, and it has a different ITS sequence. Other quite Sequences of Ramaria acrisiccescens were included as out- similar unclamped species of Ramaria are R. neoformosa and group. Number at nodes represent percentage of bootstrap R. fagetorum, but they have larger basidiomata and grow in support from parsimony (ﬁrst number) and maximum likelihood (second number) analyses; only bootstrap support > 50 % are indicated. The R. cistophila sequences are marked in bold. The Colour illustrations. Spain, Zamora, Mellanes, shrubland of Cistus accession numbers from GenBank sequences are indicated ladanifer; a. basidiome (AH 47781); b. basidiome (AH 47771); c. spores (AH at all the terminals (* after GenBank Accession Number: new 47765). Scale bar = 2 μm. sequences obtained in this study).

Pablo P. Daniëls, Departamento de Botánica, Ecología y Fisiologia Vegetal, Universidad de Córdoba, 14071 Córdoba, Spain; e-mail: [email protected] María P. Martín, Departamento de Micología, Real Jardín Botánico-CSIC, Plaza de Murillo 2, 28014 Madrid, Spain; e-mail: [email protected] Carlos Rojo De Blas, IdForest. C/ Curtidores 17, 34004 Palencia, Spain; e-mail: [email protected] Francisco Camello Rodríguez, Avda. Pozo del Paraguas 14, 06500 San Vicente de Alcántara, Badajoz, Spain; e-mail: [email protected]