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The first phylogenetic study of annulatus (: Nudibranchia) in subtidal area of Chabahar, Oman Sea, Iran, based on sequence of cytochrome oxidase C subunite I gene

Item Type article

Authors Attaran Fariman, G.; Moosavipoor, Y.

Download date 25/09/2021 16:34:19

Link to Item http://hdl.handle.net/1834/36766 1392 / 2 /

(Mollusca: Nudibranchia) Goniobranchus annulatus

I I C

*** [email protected] 1392 : 1392 : ((( Heterobranch ))) ((( )))) ((( ))) Anthobranchia ... Goniobranchus annulatus ... G. annulatus COI ... Anthobranchia .... 15151515 Neighbor _joining Downloaded from isfj.ir at 18:33 +0330 on Sunday December 24th 2017 [ DOI: 10.22092/ISFJ.2017.110121 ] 100100100 Goniobranchus vibratus Goniobranchus annulatus ... . : ﻧﺮﻣﺘﻨﺎن، ﺷﻜﻤﭙﺎﻳﺎن، رده ﺑﻨﺪي، COI, ﺳﻮاﺣﻞ ﭼﺎﺑﻬﺎر

heterobranch . (2005 Opisthobranchia . . Nudibranchia Haszprunar & Huber,) 1988 *Corresponding author 77

... Goniobranchus annulatus

. . (Thompson, 2009) Nudibranchia Opisthobranchia Euthyneura ( 2000 ) Thollesson . 2005 (. Dinapoli , 2009 ) Pulmonata 16S rRNA sidegill ) pleurobranch Dorid COI ( c 658 bp . 2010 . ( COI ) Opisthobranchia Hebert et al. , ) Nudipleura ( 2003 ( ) Euthyneura Goniobranchus . 200 . (Guido et al. , 2006) : Dorid aeolid ( Anthobranchia ) dorid ( Guido et al., 2006) (Wollscheid (et ) ( Cladobranchia ) Goniobranchus Cladobranchia Odhner, 1934 .al. , 2001) Dendronotoidea Anthobranchia . Aeolidoidea . . Doridoidea Bathydoridoidea (. Wägele, 1989 ) 5 1391 Cladobranchia Anthobranchia .( 1 ) (. Wollscheid & Wägele, 1999) 3000 . . heterobranchs DNA . Sea ) 80 . ( slug . (Thollesson, 2000) 2% ) CTAB 500 . Downloaded from isfj.ir at 18:33 +0330 on Sunday December 24th 2017 [ DOI: 10.22092/ISFJ.2017.110121 ] CTAB ,1.4M NaCl , 0.1% β_mercaptooethanol RS232C 5 20mM EDTA and 100mM TRIS-HCl pH8 ) DNA . _mercapthonal β 4/1 K DNA E_BOX_VX2/2M . 20 - . COI DNA ( 24:1 ) LCO1490f , HCO2198r DNA . (Galil et al. , 2010) DNA -20 . Ependorf 5331 1 78

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(Kumar et al. , 1994 ) 50 DNA 15 BioEdit Ver 7.0 ( Hall, 1999 ) MEGA Ver 5 . . ClustalX (Jeanmougin et al. , 1998) ,PCR 10 x DNA

Taq DNA polymeras dNTP Mgcl 2Buffer BioEdit . 95 5 PCR . Clustal X 38 DNA 1 95 . MEGA 1 45 Neighbor _joining 1 72 Nudibranch 15 . 10 72 . 1( ) PCR Mesogastropoda Littorina littorea clean up 1 . . 1: Goniobranchus annulatus , Iranian KF 408221 Goniobranchus leopardus JQ 727847 Goniobranchus hintuanensis JQ 727845 Goniobranchus vibratus JQ 727859 ambiguus EF 535119 Durvilledoris similaris EF 535128 bollandi JO 7278867 Hypselodoris apolegma JO 727886 blacki EF 142879 Tambja olivaria EF 142865 EF 142868

Downloaded from isfj.ir at 18:33 +0330 on Sunday December 24th 2017 [ DOI: 10.22092/ISFJ.2017.110121 ] Tambja ceutae AY 345038 Tambja sp. DQ 230999 Tambja eliora EF 142872 Austrodoris kerguelenensis GQ 292936 kerguelenensis GQ 292036 Littorina littorea ( out group) GQ 845183

.

79

... Goniobranchus annulatus

G. annulatus Chromodoris annulata . ( 10 ) 100 Neighbor_joining 1(. ) Goniobranchus annulatus . Goniobranchus vibratus Pease, 1860 Bergh, 1891 . G. annulatus (Synonym) . ( 2 ) Chromodoris annulata Eliot,1904 Chromodoris annulata Chromodorididae ( 1388 ) 100 Goniobranchus vibratus . Goniobranchus annulatus Downloaded from isfj.ir at 18:33 +0330 on Sunday December 24th 2017 [ DOI: 10.22092/ISFJ.2017.110121 ] . =1 . Goniobranchus annulatus 1: . 4 3 2 :1 ( _ )

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Downloaded from isfj.ir at 18:33 +0330 on Sunday December 24th 2017 [ DOI: 10.22092/ISFJ.2017.110121 ] . NJ COI 2: . Littorina littorea . replication 1000 . Goniobranchus annulatus Iranian species

81

... Goniobranchus annulatus

Chromodoris Hypselodoris ) . ( 89 Goniobranchus . Goniobranchus annulatus 15 . Nudibranchia Goniobranchus NJ . Chromodorididae . Dorid . dorid 2 Goniobranchus (G. annulatus ) Nudibranchia . vibratus Goniobranchus vibratus (Wägele, 1989;Wägele & Willan, 2000) . 18S ( 1999 ) Wägele Wollscheid 3(. ) 19 53 rDNA . chromodorid 16S rDNA ( 1999 a ) Thollesson Goniobranchus vibratus . 30 Cladobranchia . . 241 Nudibranchia Anthobranchia Wägele Wollscheid (. Wollscheid et al. , 2001 ) .(Bertsch & Johnson, 1982) . (1999 ) Chromodoris annulata . Anthobranchia Synonym ( 4 ) (G. annulatus ) Goniobranchus annulatus Goniobranchus vibratus . Chromodorididae NJ Chromodorididae . Downloaded from isfj.ir at 18:33 +0330 on Sunday December 24th 2017 [ DOI: 10.22092/ISFJ.2017.110121 ] . 18S ( 2008 ) Wilson Turner 4(. 3 ) COI rDNA, 16S rDNA

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Goniobranchus vibratus 3: :1 . =1 . ( 2 ) G. annulatus 3 2 ( www.marinelifephotography.com ) G. annulatus Chromodoris annulata 4: ( www.nudipixel.net/locations/country/ir ) =1 .

Downloaded from isfj.ir at 18:33 +0330 on Sunday December 24th 2017 [ DOI: 10.22092/ISFJ.2017.110121 ] Rudman . H .apolegma Hypselodoris bollandi (1984 ) 2( ) Chromodoris Durvilledoris Hypselodoris Goniobranchus annulatus ( 1999 b ) Thollesson Hypselodoris Chromodoris . Hypselodoris Durvilledoris Chromodoris ambiguus Hypselodoris . Durvilledoris similaris Rocroi Bouchet .Tambja Burn, 1962 83

... Goniobranchus annulatus

another alien jellyfish from the Mediterranean Polyceridae ( 2005 ) coast of Israel. Aquatic Invasions, 5(4):331– Nudipleura Euctenidiacea 340. (2006 ) Pola . Guido T. and Poppe Sheila P.T., 2006. The new Tambja classification of gastropods according to Bouchet Chromodorididae . & Rocroi, 2005; , Lapu City, Visaya . 11P. (Rafinesque, 1815) Hall T.A., 1999. BIOEDIT: A user-friendly Doris kerguelenensis Austrodoris kerguelenensis biological sequence aligment editor and analysis 100 for Windows 95/98/NT. Nucleic Symposium Chromodorididae Series (London), 41:95-98 . Haszprunar G. and Huber G., 1990. On the central nervous system of Smeagolidae and Rhodopidae, two families questionably allied with the Gymnomorpha (: Euthyneura). Science and Zoology, 220:185-199. Hebert P.D.N., Cywinska A., Ball S.L. and . deWaard J.R., 2003. Biological identifications through DNA barcodes. Proceeding of the Royal Society of London, B, 270(1512):313–321. Bertsch H. and Scott J., 1982. Three new species Jeanmougin F., Thompson J.D., Gouy M., of dorid nudibranchs (Gastropoda: Higgins D.G. and Gibson T.J., 1998. Multiple Opisthobranchia) from the Hawaiian Islands. The sequence alignment with Clustal X. Trends. Veliger, 24(3):208-218. Biochemistry Science, 23:403–405. Boettger C., 1955. Die Taxonomie der euthyneuren Jörger K. M., Stöger I., Kano Y., Fukuda H., Schnecken. Zoologischer Anzeiger, 18:253-280. Knebelsberger T. and Schrödl M., 2010. On Bouchet P. and Rocroi J.P., 2005 . Classification the origin of Acochlidia and other enigmatic and nomenclator of gastropod families. Downloaded from isfj.ir at 18:33 +0330 on Sunday December 24th 2017 [ DOI: 10.22092/ISFJ.2017.110121 ] euthyneuran gastropods, with implications for International. Journal of Malacology, 47(1-2): the systematics of Heterobranchia. BMC 257-267. Evolutionary Biology, 10:320-323. Dinapoli A., 2009. Phylogeny andevolution of the Kumar S., Tamura K. and Nei M., 1994. MEGA: Heterobranchia (Mollusca, Gastropoda). Molecular Evolutionary Genetics Analysis Frankfurt am Main. Johann Wolfgang Goethe – software for microcomputers. Computer Universität als Dissertation angenommen. 176P. Applications in the Biosciences, 10(2):189–91. Galil B.S., Gershwin L.A., Douek J. and Minichev Y.S., 1970. On the origin and system of Rinkevich B., 2010. Marivagia stellatagen . et nudibranchiate (Mollusks, Gastropoda, sp. nov. (Scyphozoa: Rhizostomeae: Cepheidae), 84

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Iranian Scientific Fisheries Journal Vol.20, No. 2, Summer 2013

The first phylogenetic study of Goniobranchus annulatus (Mollusca : Nudibranchia) in subtidal area of Chabahar, Oman Sea, Iran:, based on sequence of cytochrome

oxidase C subunite I gene

Attaran Fariman G.* and Moosavipoor Y.

[email protected] Chabahar Maritime University (CMU), Faculty of Marine Sciences, Department of Marine Biology Chabahar, Iran

Received: March 2013 Accepted: July 2013

Keywords: Gasteropoda, CO1, Chabahar coast

Abstract Nudibranches are the largest group of Hetrerobranches, (a sub-branch of Opisthobranchia) and are widespread and cosmopolitan species. They vary in shape and colors and occur in all waters from intertidal to deep sea zones and from polar to the tropics regions. Nudibranches are subdivided in two main groups including Anthobranchia and Cladobranchia. In the present study, based on morphology the svudied species was identified as Goniobranchus annulatus Downloaded from isfj.ir at 18:33 +0330 on Sunday December 24th 2017 [ DOI: 10.22092/ISFJ.2017.110121 ] that belong to Anthrobranchia group. In molecular analysis the sequences of the partial mitochondrial COI gene of Goniobranchus annulatus species from Iran with 15 similar sequence of nudibrnaches from genbank were compared. Phylogenetic based on Neighbor_joining analyze showed a monophyly relationships between families of nudibranches and also revealed that Goniobranchus vibratus is a sister taxa to the Gonidbranchus annulatus from Iran Goniobranchus annulatus with 100% bootstrap support.

*Corresponding author

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