Supplementary Information
Supplemental Methods
Tissue Culture and Reagents
All NSCLC cell lines were obtained from ATCC and maintained in RPMI-1640 medium (Invitrogen), supplemented with 10% FCS (Lonza Group) and 4 mM
GlutaMAXTM (Invitrogen). Cells at low passages were used for siRNA screen and subsequent experiments. HEK293T (293T) and H157 were maintained in DMEM medium supplemented with 10% FCS and 2 mM GlutaMAXTM. MEK inhibitor
AZD6244 (Selleck Chemicals) and FGFR inhibitor LY2874455 (Active BioChem) were dissolved in DMSO and aliquots stored at -200C. Pan-RAF inhibitor L779450
(Calbiochem) was dissolved in DMSO and stored at 40C.
Western Blotting
Cells were lysed on ice with either Triton X-100 Cell Lysis or RIPA buffer (Cell
Signaling) supplemented with protease inhibitor tablet (Roche). Lysates were resolved on SDS-PAGE gels followed by western blotting. Primary antibodies used were as follows: pMEK1/2 (S217/221), pERK1/2 (T202/Y204), ERK1/2, pAKT
(S473), pSTAT3 (Y705), pJNK (T183/Y185), MAP3K9 (MLK1) (Cell Signaling
Technology); PAK5 (Abcam); FGFR4 (c-16), BRAF (F-7), KRAS (F234), NRAS
(F155), p-Raf-1 (S338)-R (Santa Cruz Biotechnology); alpha-tubulin, Flag M2
(Sigma); and HA (Covance). Mouse or rabbit HRP-conjugated antibodies were used as secondary (Cell Signaling). All western blots are representative of at least three independent experiments. For inhibitor studies, cells were seeded 24 h prior to treatment at indicated time points and concentrations.
Cell Viability Assay Cells were reverse transfected with siRNA or treated with inhibitor for 72 h in a 96- well plate format. Cell viability was assayed with MTT Kit (3-(4,5-Dimethylthiazole -
2-yl)-2,5-diphenyltetrazolium bromide) (Sigma) according to manufacturer’s instructions.
Colony Formation Assay
Cells were seeded at approximately 3000 cells/well in a 12-well plate format. The following day, shRNA knockdown was induced with 1 mM IPTG for 72 h. Colonies formed were then fixed with ice-cold methanol for 10 mins, then stained with 0.5% crystal violet (Sigma) solution made up in 25% methanol for 10 mins. Wells were thoroughly washed and air dried. For quantification, 1 ml of 10% acetic acid was added to each well, incubated for 20 mins with shaking and absorbance values read at
590 nm.
Transient Transfections
Cells were reverse transfected with indicated siRNA in 12-well formats using
DharmaFECT1 transfection reagent unless otherwise stated. Transient siRNA knockdown was carried out for 72 h unless otherwise stated. For ectopic expressions,
293T or H157 cells were seeded at 50% confluency and the following day transfected with designated amount of plasmid DNA using Attractene transfection reagent
(Qiagen) for 48 h. Low serum conditions (0.1 % FCS) were used as indicated. Wild type constructs were purchased as entry vector (MAP3K9, Origene), shuttle clone from Gene Copoeia (FGFR4) or donor plasmid PAK5 (Addgene). Mutations were introduced by site-directed mutagenesis (QuickChange II kit, Stratagene). Gene constructs were cloned into HA- or Flag-tagged destination vectors.
Generation of IPTG-inducible shRNA Cell Lines shRNA constructs as viral particles were purchased in pLKO-puro-IPTG-3XLacO vectors from Sigma as follows: MAP3K9 sh#1 _TRCN0000196575
CCGGGCGATGAAATTGTCGTGTATGCTCGAGCATACACGACAATTTCATC
GCTTTTTTG; MAP3K9 sh#2_TRCN0000021496
CCGGGCGCTTCAAACGAGATCCTAACTCGAGTTAGGATCTCGTTTGAAGC
GCTTTTT; FGFR4 sh#1_TRCN0000000628
CCGGGCCGACACAAGAACATCATCACTCGAGTGATGATGTTCTTGTGTCG
GCTTTTT; FGFR4 sh#2_TRCN0000000629
CCGGCCTATGTGCAAGTCCTAAAGACTCGAGTCTTTAGGACTTGCACATA
GGTTTTT.
H2009 and H2122 cells were transduced with 8 µg/ml polybrene at a MOI of 2 with relevant constructs and a non-targeting control vector. Transduced cells were selected with 2 µg/ml puromycin and shRNA knockdown induced with either 800 µM or 1 mM IPTG (Sigma) for 72 h.
Reverse Transcriptase PCR
After siRNA knockdown for 48 h, cells were collected and total RNA extracted using
RNeasy RNA extraction kit (Qiagen). The quantity of RNA was determined using
NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies). RT-PCR was performed using SuperScript III One-Step RT-PCR kit (Invitrogen). Primers used were as follows:
PAK5 forward_ GTACCTGAATCAGACAAGC;
PAK5 reverse_ CCATCTGTGCTCGATCGTAA;
GAPDH forward_ CCATGGAGAAGGCTGGGG;
GAPDH reverse_GTCCACCACCCTGTTGCTGTA.
The cycling conditions for PCR were as follows: cDNA synthesis and pre- denaturation (1 cycle at 55°C for 30 min followed by 94°C for 2 min); PCR amplification (30 cycles of denaturing at 94°C for 15 s, annealing at 55°C for 30 s and extension at 68°C for 60 s) and a final extension at 68°C for 5 min.
PCR products were resolved on 2% agarose gel and visualized with Nancy-520 DNA gel stain under UV light.
Structural Modeling
Homology model of FGFR4 tyrosine kinase domain structure was based on the crystal structure of FGFR2 (pdb 2PVF; 72% homologous). Homology model of
MAP3K9 E179K mutant was based on the crystal structure of the MAP3K9 kinase domain (pdb 3DTC). Loop refinement was performed on the crystal structures of both
PAK5 kinase domain (pdb 2F57) and MAP3K9 kinase domain (pdb 3DTC) to predict the positions of missing loops, including the activation loop. Homology modeling and loop refinement was performed using Schrodinger Suite 2012/Maestro9.3.5. Images were created using PyMol version 1.3.
Molecular Dynamics (MD) Simulations
Molecular dynamics simulations were performed using GROMACS version 4.5.3
(32) on MAP3K9 wild type and MAP3K9 E179K models. The GROMOS96 53A6 force field parameter set was used and all titratable amino acids were assigned their canonical state at physiological pH. The short-range interactions were cut off at 1.4 nm, long-range electrostatics were calculated using the particle mesh Ewald summation and dispersion correction was applied to energy and pressure terms accounting for trunction of van der Waals forces. Periodic boundary conditions were applied in all directions. Protein constructs were placed in a three-dimensional box
(8 x 8 x 8 nm) of 100 nm NaCl in simple point charge water, including neutralising counterions. Energy minimisation (steepest descent) was performed, followed by a two-step NVT/NPT equilibration. Both equilibration steps maintain a constant number of particles and temperature, NVT equilibration was performed for 100 ps maintaining a constant volume followed by NPT equilibration, performed for 100 ps, maintaining a constant pressure. Temperature was maintained at 37oC by coupling protein and non-protein atoms to separate temperature coupling baths, pressure was maintained at 1.0 bar (weak coupling). All position restraints were then removed and simulations were performed for 2.5 ns using the Nosé-Hoover thermostat and the
Parrinello-Rahman barostat. Simulations were performed in triplicate, values shown in figures are averages.
MAP3K9 In Vitro Kinase Assay
Human GST-tagged MAP3K9 catalytic domain expressed using baculovirus expression system (Carna Biosciences) was incubated with kinase inactive MEK1
(Carna Biosciences). Kinase assay was performed with 200 µM ATP at 30˚C for 30 min. Following addition of 4x reduced SDS sample buffer, proteins were resolved by
SDS-PAGE and analyzed by western blotting.
Statistical Analysis
Raw data comprised biological triplicates each consisting of three technical replicates, plus a positive and negative control.
Data were normalized to the median of the negative control and scaled to a percentage. Initial data quality was assessed visually by plotting mean values between screens and calculating Pearson correlation between values. Z-scores for each candidate gene within each screen were also generated to assess the within-screen variability, using the R package Rbasic (SI Appendix, Fig. S2).
Strictly Standardized Mean Difference (SSMD) was used as a formal quality metric and also for hit selection, as recommended in (17). SSMD is a measure of effect size, it is the mean divided by the standard deviation of the difference between two random values, each from one of two groups (18). When the two populations are statistically independent, the SSMD, denoted as β, is defined as
µ − µ β = 1 2 2 2 (1) σ1 +σ 2 where µ1 and µ2 are respectively the means of the two groups, and σ1 and σ2 are the standard deviations of the two groups. In comparison with another commonly used method Z-factor, SSMD is less conservative and its interpretation is underpinned by rigorous statistics, including statistical estimators for non-normal data. Based on
SSMD's probability interpretation, a cut-off criterion was proposed for hit selection using SSMD (19), which were used in our experiments. We used the uniformly minimal variance unbiased estimate (UMVUE) of SSMD on the paired data, which has the formula (16)
n−1 ( ) ˆ Γ 2 2 di βi = n−2 (2) ( ) Γ 2 n −1 si
where n is the number of replicates in one cell-line, Γ() is the Gama function, di and si are respectively the sample mean and standard deviation of dijs where dij is the difference between the measured activity value on the log2 scale of the ith siRNA in the jth replicate and the median value of the negative control. We calculated the
UMVUE estimate of SSMD for each siRNA and for each cell line, which we implemented in R.
Supplemental Figure and Table Legends
Fig. S1. siRNA targeted screen experimental design, target identification and optimized transfection conditions. (A) Custom designed siRNA SMARTpool library was obtained as 96-well master plates for each NSCLC cell line (SI Appendix, Table
S9). Library was designed to known somatic mutations documented in COSMIC database for each cell line (SI Appendix, Table S1). Each well contained a mix of 4 siRNA oligos targeting one mutated gene (SMARTpool). Lyophilised siRNA oligos were resuspended in nuclease free water, seeded into multiple 96-well daughter plates and stored at -200C. For experimental set up, on day 0 siRNA and transfection reagent were prepared in OPTI-MEM using optimised conditions and arrayed on assay plates excluding outside wells to avoid edge effect discrepancies during incubation. Control siRNA were randomly distributed across assay plates. Cells were seeded over transfection mix (reverse transfection) and media changed after 24 h to remove transfection reagent. On day 3, cells were either assayed for BrdU incorporation by 24 h BrdU labelling or apoptosis by measuring caspase 3/7 activity
(H2087 cell line). Assays were carried out in triplicate wells with at least three independent experimental set up. (B) Data were analysed by normalisation to in-plate negative control (SiNeg). Mean values for each target gene knocked down was calculated and z-scores calculated to account for assay reproducibility. The threshold for hit selection was determined by SSMD values (Fig. 2). A SSMD ranked gene list was generated for each cell line (Tables S2-S9). (C,D) Summary of panel of six
NSCLC cell lines used in siRNA targeted screen. Each cell line was derived from different stages of lung cancer and harbors different number of somatic mutations, some of which are activating drivers of cancer. Data compiled from ATCC and COSMIC databases. NSCLC cell lines used in siRNA targeted screen illustrate differences in morphology and growth pattern. Scale bars represent 50 µm. (E)
Optimised transfection efficiency in 96-well format for each NSCLC cell line screened. DharmaFECT1 (DF1) transfection reagent was the most efficient lipofection reagent at indicated volumes for each cell line at optimised seeding density for 72 h knockdown. Percentage viability was assayed by Hoescht 33342 staining. Percentage efficiency was calculated by densitometry using ImageJ based on
Cyclophilin B protein knockdown, normalised to tubulin (Fig. 1B) (n=3). *Cell lines recalcitrant to transient transfection. Cells were used for transfection at a maximum of one passage after culture induction, at lowest seeding density and maximal transfection reagent concentration.
Fig. S2. Mean scatter values comparing screen pairs and screen reproducibility, z- score scatter for each screen and SSMD hit selection scatter for (A,B,C) H2087 BrdU,
(D,E,F) H2122 BrdU, (G,H,I) H2009 BrdU and (J,K,L) H2087 Caspase assay.
Fig. S3. Validation of FGFR4 as an activating mutant in H2122. (A) H2122 cells were reverse transfected with control non-targeting siRNA Neg, FGFR4 SMARTpool
(100 nM) or FGFR4 single oligo (25 nM each) in 96-well plates for 72 h and cell viability assessed by MTT assay (n=9). Error bars showing standard deviations and P values calculated using one-tail paired student’s t-test. *P<0.001, **P<0.05 vs NEG control. (B) H2122 cells were reverse transfected with siFGFR4 (SP:100 nM, oligo 1-
4: 25nM) and downstream substrates analysed by western blotting as indicated.
(FGFR4 unmodified at 88 kDa and modified at 125 kDa.) (C) H2122 cells shRNA
IPTG-inducible cells were generated and FGFR4 knockdown induced with 800 µM IPTG for 72 h. Lysates were analysed by western blotting. (D) FGFR4 WT, kinase dead (KD) K503M and P712T mutants were expressed ectopically with 0.1 ng of plasmid DNA in H157 cells for 48 h in low serum conditions, with or without treatment with FGFR inhibitor LY2874455, 30 min prior to lysis. Lysates were collected and analysed by western blotting to evaluate pERK activation relative to
WT and KD. (E) H2122 cells were treated with LY2874455 inhibitor for the indicated time points and lysates analysed by western blotting. (F) Downstream substrate phosphorylation was analysed by transiently knocking down MAP3K9 for
24 h in H2009 cells (Pool: 100 nM; Oligos 1 to 4: 50 nM). Total MEK and tubulin were used as loading controls.
Table S1. List of somatic nonsynonymous mutations for six NSCLC cell lines compiled from Catalogue Of Somatic Mutations In Cancer and Cancer Cell Line
Encyclopaedia.
Table S2. Gene list ranked by SSMD values for H2087 BrdU targeted screen assay.
Hit selection was carried out as indicated.
Table S3. Gene list ranked by SSMD values for H2122 BrdU targeted screen assay.
Hit selection was carried out as indicated.
Table S4. Gene list ranked by SSMD values for H2009 BrdU targeted screen assay.
Hit selection was carried out as indicated.
Table S5. Gene list ranked by SSMD values for H2126
Table S6. Gene list ranked by SSMD values for H1395
Table S7. Gene list ranked by SSMD values for H1437
Table S8. Gene list ranked by SSMD values for H2087 apoptosis targeted screen assay. Hit selection was carried out as indicated. Table S9. List of siRNA oligonucleotides used for targeted screen and deconvolution.
Supplementary Table 1
List of missense somatic mutations compiled for siRNA targeted screen NCI-H1395 http://www.sanger.ac.uk/perl/genetics/CGP/cosmic?action=sample&id=684681 Gene Amino acid change Nucleotide change ATM p.T2666A c.7996A>G BIRC6 p.T343S c.1027A>T BRAF p.G469A c.1406G>C CENPC1 p.V827L c.2479G>T CXCR2 p.G354W c.1060G>T DDX23 p.R73W c.217C>T DOT1L p.G1052V c.3155G>T ERN1 p.L474R c.1421T>G FBXO25 p.S190S c.570T>A FLCN p.L449fs*25 c.1334_1335ins17 GHR p.V343I c.1027G>A GNB5 p.A200S c.598G>T GPC3 p.D164H c.490G>C HMGN5 p.E258* c.772G>T IMPDH2 p.D226H c.676G>C MYCBP2 p.G1204L c.3610_3610GG>TT MYO3B p.S867* c.2600C>A MYST2 p.G505S c.1513G>A NLRP1 p.K270* c.808A>T NLRP4 p.W111L c.332G>T NUP214 p.E1580* c.4738G>T PDCD5 p.R55S c.165G>T PPARGC1A p.Q431H c.1293G>C PPIL5 p.R172L c.515G>T PROM1 p.A178>L c.532_533GC>TT PTPRT p.Q479E c.1435C>G RGAG1 p.S382S c.1146T>C SERPINA2 p.E273* c.817G>T SMURF2 p.R592* c.1774C>T SOS1 p.Y1031fs*1 c.3092_3093insA STK11 p.E57fs*7 c.169delG TRIM36 p.S447C c.1339A>T ZC3H12B p.E647D c.1941G>T
NCI-H1437 http://www.sanger.ac.uk/perl/genetics/CGP/cosmic?action=sample&id=687794 Gene Amino acid change Nucleotide change ARFGEF1 p.Q1001H c.3003G>T CDC5L p.D514H c.1540G>C CDKN2A p.0? c.1_471del471 DACT1 p.D539N c.1615G>A EIF2AK2 p.L439V c.1315C>G FAM46D p.R62I c.185G>T FASTK p.V424L c.1270G>C FBXO40 p.P687A c.2059C>G FLJ44635 p.F134L c.402C>G GPRASP1 p.K1239fs*3 c.3717_3718delAG GRIN2B p.R92L c.275G>T HDAC9 p.S532R c.1594A>C HUS1 p.S125I c.374G>T INCENP p.S235Y c.758C>A INTS2 p.R73L c.218G>T IRAK2 p.S249L c.746C>T ITGA2B p.S415I c.1244G>T ITM2A p.G51>V c.152_153GG>TT KLK15 p.I218N c.653T>A LRGUK p.D199E c.597T>G MAPK15 p.A435fs*57 c.1302_1303insC MOS p.A123T c.367G>A NCK2 p.D89Y c.265G>T NEK1 p.A294P c.880G>C NIPBL p.S349C c.1045A>T NLRP1 p.W797R c.2389T>A NLRP14 p.L749F c.2247G>T NTRK3 p.V307L c.919G>C PABPC5 p.W163C c.489G>C POLG p.E1000K c.2998G>A PPP1R3A p.S848Y c.2543C>A RAG1 p.K621N c.1863G>T RGS6 p.G462V c.1385G>T SENP5 p.K486K c.1458G>A SERPINA12 p.Q142R c.425A>G SESN2 p.R329K c.986G>A TCF12 p.E95* c.283G>T TP53 p.R267P c.800G>C TRIM9 p.D606H c.1816G>C UBE2O p.R569L c.1706G>T UBR5 p.V968I c.2902G>A WNK2 p.S1978I c.5933G>T ZNF608 p.S1428N c.4283G>A NCI-H2009 http://www.sanger.ac.uk/perl/genetics/CGP/cosmic?action=sample&id=724873 http://www.broadinstitute.org/ccle/search/searchResult# Gene Amino acid change Nucleotide change ADAM32 p.G202C c.604G>T ADAMTS15 p.Y799F c.2396A>T ADAMTS2 p.H209N c.625C>A ADAMTS20 p.M1554I c.4662G>A ALOX15 p.Q358* c.1072C>T ANK1 p.V633L c.1897G>T ANKHD1 p.M1391I c.4173G>C APAF1 p.K131M c.392A>T ASB15 p.D8N c.22G>A ASH2L p.E59K c.175G>A ATF7IP p.E372* c.1114G>T BACH2 p.Q399Q c.1197G>A BCL2L13 p.? c.230-3C>T BIRC6 p.A3656fs*3 c.10965_10977del13 BRCC3 p.L291V c.871C>G CAMK4 p.E150G c.449A>G CDK5RAP2 p.T962S c.2884A>T CHD1 p.D1680Y c.5038G>T CHD6 p.E1954K c.5860G>A CHD9 p.Q894E c.2680C>G CIT p.V112G c.335T>G CLCN5 p.? c.1933+-2delTTGGTAA CSK p.S2* c.5C>G CTNNA3 p.E281* c.841G>T CXorf23 p.R88K c.263G>A DGKB p.P568S c.1702C>T EPHA8 p.R198L c.593G>T EPHB1 p.P420fs*28 c.1259delC ERBB2IP p.K560R c.1679A>G FAM123B p.S46L c.137C>T FBXL7 p.P238S c.712C>T FBXO34 p.T230S c.688A>T FGD2 p.D125Y c.373G>T FGFR2 p.R612T c.1835G>C FGFR3 p.T79S c.235A>T FRMPD3 p.D863E c.2589C>A GLRA2 p.A288S c.862G>T GOLGA5 p.G87A c.260G>C GUCY2F p.K1052R c.3155A>G HEPH p.E909V c.2726A>T HERC1 p.E1901* c.5701G>T HIF1A p.Q697E c.2089C>G HSPA14 p.Y286H c.856T>C IL3RA p.R185P c.554G>C INSRR p.E278Q c.832G>C IPO11 p.H230D c.688C>G IRAK1 p.S690G c.2068A>G ITGA8 p.P97T c.289C>A ITGB4 p.K1142N c.3426G>C KCNV1 p.L335F c.1003C>T KDM3B p.E460K c.1378G>A KRAS p.G12A c.35G>C MAGEB16 p.T302R c.905C>G MAP3K9 p.E179K c.535G>A MBD4 p.M473R c.1418T>G MLL2 p.Q3775R c.11324A>G MOBKL2A p.F16L c.48C>G MYH9 p.H686Y c.2056C>T NCOA2 p.Q1032E c.3094C>G NLRP10 p.S405Y c.1214C>A NLRP3 p.G226A c.677G>C NTHL1 p.R42K c.125G>A NTRK2 p.L138F c.412C>T NUP160 p.D1333H c.3997G>C NUP93 p.R525Q c.1574G>A ODZ1 p.R2636L c.7907G>T PDK3 p.S362* c.1085C>G PIK3C2B p.*1635S c.4904G>C PIK3C3 p.E711D c.2133G>C PIP5KL1 p.E90K c.268G>A PLCB1 p.Q1013H c.3039G>T PLCG1 p.S489C c.1466C>G PPARGC1A p.P267R c.800C>G PPFIA3 p.S765L c.2294C>T PREX1 p.K97N c.291G>T PREX2 p.S926Y c.2777C>A PRG-3 p.Y34N c.100T>A PRKDC p.K263N c.789G>C PSKH2 p.S116I c.347G>T PTPRB p.L412P c.1235T>C PTPRM p.I1037K c.3110T>A PTPRT p.K788E c.2362A>G PTPRZ1 p.R481R c.1441C>A RAD21 p.Q474* c.1420C>T RAD50 p.? c.1219-1G>T RAD51L3 p.G177V c.530G>T RAPGEF6 p.D1130H c.3388G>C RB1 p.? c.1696_2787del1092 RBBP5 p.D507fs*22 c.1515delA RBL1 p.W568C c.1704G>C REST p.Q622R c.1865A>G RET p.R348R c.1044G>A RHOBTB3 p.D72H c.214G>C RPS6KA2 p.S427* c.1280C>G SCYL2 p.Q863H c.2589G>T SLC4A10 p.P690L c.2069C>T SMARCC2 p.I901M c.2703C>G SMARCD3 p.D161H c.481G>C TAF1L p.L762I c.2284C>A TIAM1 p.H847Y c.2539C>T TIMP3 p.S179P c.535T>C TNK2 p.R34L c.101G>T TNKS2 p.P318A c.952C>G TOP2B p.R312G c.934A>G TP53 p.R273L c.818G>T TPO p.N557Y c.1669A>T TRIM26 p.V439L c.1315G>T TRIM33 p.E811K c.2431G>A TRPS1 p.R284P c.851G>C TSHZ3 p.G693V c.2078G>T TYRP1 p.A24S c.70G>T UBR5 p.W395C c.1185G>T USP38 p.D939Y c.2815G>T WDR44 p.E69D c.207G>C WHSC1 p.E563fs*>23 c.1685_1686insC WIF1 p.I378S c.1133T>G XIAP p.L256V c.766C>G ZFYVE9 p.S1189G c.3565A>G
NCI-H2087 http://www.sanger.ac.uk/perl/genetics/CGP/cosmic?action=sample&id=724834 Gene Amino acid change Nucleotide change AATK p.L97V c.289C>G ADAMTSL3 p.W343C c.1029G>C ADH7 p.E166* c.496G>T ATM p.E848Q c.2542G>C BAI3 p.Y1343N c.4027T>A BRAF p.L597V c.1789C>G CCNG2 p.W244C c.732G>C CCNT1 p.Q627L c.1880A>T CHD1L p.D796N c.2386G>A CSMD3 p.P3513S c.10537C>T CSMD3 p.S2031* c.6092C>G DGKB p.E48K c.142G>A DGKD p.A450S c.1348G>T DOCK1 p.E1098V c.3293A>T EPHA5 p.R417Q c.1250G>A ERBB2IP p.E508K c.1522G>A ERC1 p.L583I c.1747C>A FBLN5 p.P105T c.313C>A FBXO34 p.R437I c.1310G>T FBXO46 p.T396M c.1187C>T FOXP2 p.Q150L c.449A>T FZR1 p.V253A c.758T>C GATA2 p.P125T c.373C>A GLP1R p.S432R c.1296C>A GPR50 p.M155I c.465G>T HDAC7 p.D850E c.2550C>G HECW1 p.H821Q c.2463C>A HEPH p.L1139I c.3415C>A HERC6 p.W794* c.2382G>A IKZF3 p.I338M c.1014C>G ITGA10 p.L1139V c.3415C>G ITGA2 p.S88F c.263C>T ITGA6 p.K472* c.1414A>T ITGA8 p.P900L c.2699C>T ITPR2 p.R333S c.999A>T KNTC1 p.V1287L c.3859G>T KSR2 p.S676R c.2028C>A LHFP p.H55N c.163C>A LOC389904 p.P549T c.1645C>A LRGUK p.G426E c.1277G>A MAGEB6B p.G71F c.211_212GG>TT MCF2 p.M676I c.2028G>T MEFV p.G660* c.1978G>T MEX3D p.Q87* c.259C>T MYH11 p.K1284Q c.3850A>C NCOA7 p.W804* c.2412G>A NLRP13 p.E726K c.2176G>A NLRP4 p.P148Q c.443C>A NOS1AP p.A53S c.157G>T NOTCH4 p.L607I c.1819C>A NRAS p.Q61K c.181C>A NTRK3 p.H677Y c.2029C>T NUP153 p.F790L c.2370C>G ORC6L p.K201N c.603G>C PAK7 p.T538N c.1613C>A PLS3 p.N290Y c.868A>T PPP1R10 p.E445K c.1333G>A PTPRT p.R1373C c.4117C>T RAF1 p.Q335H c.1005G>C RNF130 p.H86Y c.256C>T SERPINA13 p.F58L c.174C>A SERPINB4 p.N220T c.659A>C SGK2 p.E259K c.775G>A SGMS2 p.K323N c.969A>C SLK p.Q405K c.1213C>A SPEN p.H197Y c.589C>T SPEN p.T907I c.2720C>T TEC p.R563K c.1688G>A TGS1 p.E516Q c.1546G>C TP53 p.V157F c.469G>T TPR p.S1759F c.5276C>T ULK1 p.S784C c.2351C>G ZFYVE9 p.D479E c.1437T>G ZNF630 p.S111L c.332C>T NCI-H2122 http://www.sanger.ac.uk/perl/genetics/CGP/cosmic?action=sample&id=722046 Gene Amino acid change Nucleotide change ARHGAP6 p.G130S c.388G>A CDH1 p.E165_P170del c.493_510del18 CDKN2A p.? c.1_150del150 CREBBP p.N83T c.248A>C CSNK1E p.R256L c.767G>T CYP24A1 p.L290_Y293>Y c.869_877delTAGAGAAGT DHX32 p.G111R c.331G>C DIAPH2 p.E86D c.258G>C FBXL21 p.G63R c.187G>A FGFR4 p.P672T c.2014C>A GLI3 p.P940T c.2818C>A HEPH p.G702>F c.2104_2105GG>TT KRAS p.G12C c.34G>T NLRP10 p.S287F c.860C>T NYX p.G452R c.1354G>C PARP1 p.G913fs*4 c.2738delG PI4K2A p.V298M c.892G>A PIGC p.P25S c.73C>T RASL10B p.E28K c.82G>A STAG3 p.F660L c.1980C>A STK11 p.P281fs*6 c.842delC TMEM164 p.R38L c.113G>T TP53 p.Q16L c.47A>T NCI-H2126 http://www.sanger.ac.uk/perl/genetics/CGP/cosmic?action=sample&id=687814 Gene Amino acid change Nucleotide change ADAMTS20 p.P1250T c.3748C>A ADHFE1 p.P383fs*3 c.1147_1148>T AFF3 p.G1222>V c.3665_3666GG>TT ALDOA p.R22C c.64C>T ANK1 p.L1061V c.3181C>G ATR p.A2002G c.6005C>G AURKC p.G18E c.53G>A BAAT p.A182S c.544G>T BRCC3 p.? c.403+1G>T BRE p.R9L c.26G>T C11orf92 p.E109fs*1 c.323_324insC CALCR p.Y41* c.123C>G CAMK2G p.? c.66-1G>T CDKN2A p.0? c.1_471del471 CDKN2a(p14) p.0? c.1_522del522 COL4A1 p.G1242fs*18 c.3724_3731delGGCCAGCC CSMD3 p.N409D c.1225A>G CXorf15 p.H486Y c.1456C>T CXorf30 p.D616Y c.1846G>T CXorf57 p.P434R c.1301C>G DARC p.F81V c.241T>G DCN p.N255K c.765C>A DDX51 p.P132L c.395C>T DET1 p.M294_A295>IS c.882_883GG>TT DGKB p.A736S c.2206G>T DGKB p.D592Y c.1774G>T DNAJB11 p.E205* c.613G>T E2F3 p.G184C c.550G>T EIF2S3 p.? c.261+5G>A EPHA3 p.G766E c.2297G>A ETV1 p.L79M c.235T>A F9 p.S156T c.466T>A FAM70A p.G202G c.606G>A FBXL12 p.R33C c.97C>T FGF3 p.S223C c.668C>G FRK p.Y193* c.579C>A FRMPD4 p.D315E c.945T>A HDAC9 p.P448P c.1344C>A HERC2 p.A3491V c.10472C>T HES1 p.A179>S c.534_535CG>TT IKZF1 p.R433S c.1297C>A INPP1 p.C280F c.839G>T INPP4B p.S794S c.2382A>G INTS6 p.H555Y c.1663C>T IRS4 p.A972>V c.2915_2916CA>TT ITGA4 p.P11L c.32C>T ITGA9 p.D377Y c.1129G>T ITIH5L p.A575E c.1724C>A KDR p.Q2R c.5A>G KIF16B p.R803L c.2408G>T KLHL10 p.C446C c.1338C>T KLK10 p.W268C c.804G>C KREMEN1 p.V93L c.277G>T LATS2 p.G40E c.119G>A LNX2 p.D517N c.1549G>A LOX p.L245Q c.734T>A LRP5 p.G1254>T c.3760_3761GG>AC MAPK14 p.P322R c.965C>G MCM3AP p.A309fs*21 c.924_925>C MED13L p.T2161P c.6481A>C MYCBP2 p.Q1288H c.3864A>T NAP1L2 p.G59V c.176G>T NCAPD3 p.E48G c.143A>G NHEJ1 p.Q32H c.96G>T NTRK3 p.L336Q c.1007T>A NUP54 p.T416A c.1246A>G NXF3 p.T9K c.26C>A NYX p.R268H c.803G>A PAX7 p.R89R c.267C>A PDZRN4 p.R368K c.1103G>A PFKP p.A537S c.1609G>T PGR p.D697Y c.2089G>T PIP4K2C p.D310Y c.928G>T PLK2 p.G92S c.274G>A PLXNB3 p.K503N c.1509G>T POLE p.G24fs*30 c.71delG PRKD2 p.G848E c.2543G>A PTCH2 p.A68fs*4 c.202delG PTPN9 p.R72Q c.215G>A PTPRO p.L119Q c.356T>A RAP2B p.K5T c.14A>C RARA p.A255T c.763G>A RASA2 p.E759K c.2275G>A RASA3 p.G29V c.86G>T RGS14 p.A252V c.755C>T SGK3 p.? c.873+1G>T SIK1 p.S719fs*31 c.2154_2176delGTCCCCGGTGGCCTCA SMARCA4 p.W764R c.2290T>A SMC2 p.V1037L c.3109G>T SMYD1 p.E260fs*8 c.779_795delAAGAACGCAAGAGGCAG STK11 p.? c.465_862del398 TAF1L p.E794D c.2382A>C TAOK1 p.? c.1339-2A>T TCEAL5 p.Q197K c.589C>A TMEM47 p.G87C c.259G>T TP53 p.E62* c.184G>T TPR p.S1825N c.5474G>A TRIM37 p.D497V c.1490A>T TRPM6 p.G75V c.224G>T TTN p.L7304V c.21910C>G UNC5B p.T138I c.413C>T USP31 p.P769L c.2306C>T USP44 p.R319I c.956G>T VIM p.A232S c.694G>T WDR83 p.E144Q c.430G>C WNK1 p.F2362L c.7086C>A ZNF521 p.E494* c.1480G>T Supplementary Table S2 H2087 BrdU Assay
Fold Normalised Gene change SSMD mean (log2) PAK5 56.0 -0.84 -13.96 β≤-6.67 BRAF 59.2 -0.76 -13.41 SGMS2 76.0 -0.40 -6.18 CHD1L 57.1 -0.82 -5.61 GATA2 85.9 -0.22 -5.24 -6.67<β≤-4.7 ZFYVE9 79.9 -0.33 -5.05 KIF11 55.1 -0.87 -4.75 NRAS 67.3 -0.58 -4.59 PPP1R10 69.3 -0.53 -4.56 NOS1AP 72.1 -0.48 -4.55 FOXP2 96.1 -0.06 -4.20 IKZF3 82.0 -0.29 -4.18 LRGUK 76.9 -0.38 -3.86 LOC389904 81.3 -0.30 -3.81 -4.7<β≤-3 LHFP 83.0 -0.27 -3.64 ITGA10 83.4 -0.26 -3.40 CCNT1 86.4 -0.21 -3.32 RNF130 47.1 -1.12 -3.27 CCNG2 92.1 -0.12 -3.15 FBX034 64.6 -0.65 -3.13 BAI3 80.0 -0.33 -3.02 CRAF 71.9 -0.48 -3.02 FBX046 74.5 -0.44 -2.78 MAGEB6B 88.7 -0.18 -2.78 HEPH 85.0 -0.24 -2.78 NOTCH4 84.4 -0.25 -2.66 SHARP 78.4 -0.36 -2.65 NUP153 73.5 -0.46 -2.59 ULK1 60.0 -0.76 -2.55 CSMD3 77.8 -0.37 -2.52 SLK 86.7 -0.21 -2.38 ITGA2 76.8 -0.39 -2.31 HERC6 77.1 -0.38 -2.27 TGS1 80.3 -0.32 -2.03 KSR2 91.1 -0.14 -1.97 NCOA7 89.9 -0.16 -1.91 SGK2 72.9 -0.47 -1.86 ! NTRK3 79.3 -0.35 -1.82 !
Supplementary Table S2 (continued) H2087 BrdU Assay
Fold Normalised Gene change SSMD mean (log2) HDAC7 87.9 -0.19 -1.82 ITPR2 86.7 -0.21 -1.71 ZNF630 64.7 -0.66 -1.70 TEC 82.2 -0.29 -1.57 TPR 92.9 -0.11 -1.54 ELK5 95.1 -0.08 -1.54 ATM 89.2 -0.17 -1.52 NLRP4 82.6 -0.28 -1.45 ORC6L 91.3 -0.13 -1.27 TP53 88.2 -0.19 -1.27 HECW1 91.1 -0.15 -1.07 SERPINB4 89.9 -0.16 -0.93 PLS3 92.6 -0.12 -0.93 NEG 99.8 -0.01 -0.20 AATK 101.3 0.01 0.03 MEFV 101.2 0.01 0.32 FBLN5 101.7 0.02 0.32 PTPRT 107.4 0.10 0.96 GLP1R 111.5 0.15 1.00 MYH11 105.8 0.08 1.16 NLRP13 107.1 0.10 1.29 DGKD 113.8 0.18 1.41 ERB2IP 105.2 0.07 1.43 KNTC1 114.5 0.19 2.19 ADH7 122.9 0.29 2.22 ITGA8 119.0 0.25 2.41 MEX3D 108.0 0.11 3.00 GPR50 117.9 0.23 3.67 MCF2 113.2 0.18 3.76 SERPINA13 154.0 0.61 3.93 EPHA5 127.1 0.34 4.33 DOCK1 129.6 0.37 4.35 ADAMTSL3 171.4 0.77 5.67 ITGA6 116.5 0.22 5.75 DGKB 152.2 0.60 9.91 FZR1 212.7 1.09 16.33
Supplementary Table S3 H2122 BrdU Assay ! Normalised Fold change Gene SSMD mean (log2) KIF11 23.22 -2.13 -17.42 PARP1 24.22 -2.07 -17.15
STAG3 14.78 -2.79 -14.99 β≤-6.67 NYX 39.78 -1.36 -8.33 FGFR4 16.11 -2.68 -8.23 DHX32 22.56 -2.19 -7.92 RASL10B 49.44 -1.04 -7.87 PIGC 51.11 -1.00 -6.00 NALP10 45.89 -1.16 -5.24 -6.67<β≤-4.7 PI4K2A 71.11 -0.52 -5.06 FBXL21 49.89 -1.04 -4.84 HEPH 60.22 -0.76 -4.33 FLJ22679 70.00 -0.54 -4.10 KRAS 40.22 -1.37 -3.87 -4.7<β≤-3 CDH1 46.44 -1.15 -3.79 DIAPH1 29.00 -1.88 -3.63 CSNK1E 42.11 -1.32 -3.12 CYP24A1 34.00 -1.65 -2.97 GLI3 36.33 -1.56 -2.74 TSK11 49.44 -1.08 -2.66 ARHGAP6 64.56 -0.67 -2.60 TP53 79.22 -0.37 -2.11 CDKN2A 57.78 -0.88 -1.82 CREBBP 86.78 -0.23 -1.64 ! NEG 100.89 -0.01 -0.31 ! !
Supplementary Table S4 H2009 BrdU Assay
Fold Normalised Gene change SSMD mean (log2) NALP10 27.56 ,1.90 ,8.21 APAF1 38.55 ,1.41 ,8.07 β≤-6.67 FBXL7 53.54 ,0.94 ,6.79 RAD50 38.61 ,1.42 ,6.64 MYH9 31.05 ,1.74 ,6.55 PRG,3 40.81 ,1.34 ,6.45 CAMK4 40.93 ,1.33 ,6.33 MLL2 33.23 ,1.64 ,6.11 KCNV1 33.10 ,1.64 ,6.07 GUCY2F 33.21 ,1.64 ,5.78 -6.67<β≤-4.7 CSK 47.19 ,1.13 ,5.54 MAP3K9 30.72 ,1.76 ,4.93 KRAS 47.09 ,1.13 ,4.89 RBL1 58.17 ,0.82 ,4.84 ITGA8 34.32 ,1.60 ,4.79 ITGB4 30.35 ,1.79 ,4.77 IPO11 33.48 ,1.64 ,4.67 IRAK1 35.71 ,1.55 ,4.51 INSRR 45.26 ,1.19 ,4.30 HIF1A 49.21 ,1.07 ,4.27 DGKB 38.96 ,1.42 ,4.22 GLRA2 41.59 ,1.32 ,4.19 CLCN5 47.47 ,1.13 ,4.10 RET 44.55 ,1.22 ,3.99 TAF1L 50.92 ,1.02 ,3.74 FGFR3 42.13 ,1.31 ,3.71 ANKHD1 42.24 ,1.31 ,3.66 CIAS1 33.22 ,1.67 ,3.65 PPARGC1A 51.58 ,1.00 ,3.64 ADAMTS2 45.66 ,1.19 ,3.62 FGFR2 48.33 ,1.10 ,3.57 IL3RA 56.85 ,0.86 ,3.57 CDK5RAP2 40.21 ,1.39 ,3.55 MAGEB16 45.54 ,1.20 ,3.53 TNK2 41.93 ,1.32 ,3.53 PTPRZ1 54.58 ,0.92 ,3.53 SMARCC2 37.21 ,1.50 ,3.52 ADAM32 54.53 ,0.92 ,3.49 PREX2 40.76 ,1.36 ,3.44 PREX1 39.60 ,1.41 ,3.38 ! EPHA8 29.34 ,1.88 ,3.37 Supplementary Table S4 (continued) H2009 BrdU Assay Fold Normalised Gene change SSMD mean (log2) CTNNA3 42.69 +1.30 +3.35 ERBB2IP 35.78 +1.57 +3.31 MBD4 50.45 +1.04 +3.28 WDR44 36.81 +1.52 +3.26 ASB15 33.65 +1.68 +3.20 BRCC3 44.45 +1.24 +3.20 MOBKL2A 38.66 +1.45 +3.20 SLC4A10 38.86 +1.44 +3.13 HSPA14 51.38 +1.02 +3.11 NCOA2 35.78 +1.57 +3.09 SCYL2 40.83 +1.37 +3.08 GOLGA5 40.99 +1.37 +3.06 ODZ1 39.12 +1.44 +3.06 TPO 33.52 +1.68 +3.00 BCL2L13 52.46 +0.99 +2.98 NOTCH2 50.85 +1.04 +2.95 RPS6KA2 44.43 +1.25 +2.93 PTPRB 53.23 +0.97 +2.92 KIF11 36.00 +1.58 +2.83 CXorf23 47.91 +1.13 +2.83 RAD21 43.67 +1.28 +2.72 ALOX15 45.06 +1.24 +2.72 EPHB1 45.80 +1.21 +2.71 TIAM1 43.33 +1.30 +2.71 NUP93 44.76 +1.24 +2.67 HEPH 37.86 +1.51 +2.64 NRBP1 56.30 +0.89 +2.63 FGD2 54.59 +0.94 +2.60 REST 47.60 +1.15 +2.60 FRMPD3 44.78 +1.26 +2.58 BIRC4 38.41 +1.49 +2.56 TOP2B 35.07 +1.64 +2.54 ANK1 44.88 +1.25 +2.51 PIK3C3 39.85 +1.43 +2.49 CHD9 30.84 +1.86 +2.44 USP38 47.84 +1.14 +2.44 KDM3B 57.58 +0.86 +2.44 NTHL1 49.79 +1.09 +2.39 CHD6 41.44 +1.38 +2.38 ADAMTS20 50.87 +1.06 +2.38 TRIM26 35.48 +1.62 +2.38 PDK3 40.92 +1.40 +2.36 RHOBTB3 44.96 +1.25 +2.34 ! !
Supplementary Table S4 (continued) H2009 BrdU Assay Fold Normalised Gene change SSMD mean (log2) PIP5KL1 68.02 ,0.61 ,2.31 FAM123B 36.83 ,1.59 ,2.26 NUP160 47.12 ,1.19 ,2.26 PTPRT 61.84 ,0.75 ,2.25 PIK3C2B 36.67 ,1.60 ,2.23 PTPRM 52.08 ,1.02 ,2.22 W1F1 42.72 ,1.34 ,2.20 PLCG1 58.44 ,0.84 ,2.18 RAD51L3 44.77 ,1.27 ,2.14 ATF7IP 64.42 ,0.69 ,2.14 TP53 42.41 ,1.37 ,2.09 UBR5 46.43 ,1.21 ,2.08 PSKH2 54.20 ,0.96 ,2.06 ASH2L 37.45 ,1.60 ,2.04 RAPGEF6 56.40 ,0.91 ,1.99 BIRC6 54.96 ,0.95 ,1.95 TYRP1 59.49 ,0.82 ,1.92 CIT 62.55 ,0.75 ,1.91 HERC1 53.54 ,0.99 ,1.90 PPFIA3 46.31 ,1.26 ,1.84 TIMP3 50.80 ,1.09 ,1.81 WHSC1 46.22 ,1.26 ,1.80 PLCB1 60.29 ,0.81 ,1.79 CHD1 44.93 ,1.32 ,1.73 RBBP5 65.17 ,0.69 ,1.64 TNKS2 37.75 ,1.66 ,1.59 TRIM33 57.54 ,0.90 ,1.59 ADAMTS15 41.41 ,1.48 ,1.53 FBX034 66.48 ,0.67 ,1.53 BACH2 60.36 ,0.85 ,1.51 TSHZ3 58.86 ,0.88 ,1.50 SMARCD3 45.68 ,1.35 ,1.49 TRPS1 57.22 ,0.93 ,1.46 NTRK2 55.40 ,1.01 ,1.40 MYO3B 63.60 ,0.76 ,1.34 PRKDC 66.08 ,0.69 ,1.32 ZFYVE9 69.48 ,0.62 ,1.28 RB1 71.52 ,0.60 ,0.95 NEG 100.00 ,0.04 ,0.20 ! !
Supplementary Table S5 H2126 BrdU Assay
Normalised Fold change Gene SSMD mean (log2) FRMPD4 70.8 -0.50 -10.54 KIF11 62.7 -0.67 -9.54 SIK1 62.5 -0.68 -8.67 HES1 78.9 -0.34 -8.47 PGR 71.4 -0.48 -7.89 LNX2 67.6 -0.56 -7.63 HDAC9 76.0 -0.39 -7.59 ETV1 77.1 -0.37 -7.27 MAPK14 64.1 -0.64 -7.26 AFF3 68.1 -0.55 -7.24 LRP5 74.5 -0.42 -7.21 AURKC 63.4 -0.66 -6.62 WNK1 76.8 -0.38 -6.27 COL4A1 65.4 -0.61 -6.24 DGKB 77.2 -0.37 -6.14 FGF3 78.0 -0.36 -6.02 EIF2S3 72.4 -0.47 -6.01 FBXL12 74.2 -0.43 -5.97 SMARCA4 60.4 -0.73 -5.93 MYCBP2 69.4 -0.53 -5.66 POLE 72.6 -0.46 -5.43 PDZRN4 80.3 -0.32 -5.43 LATS2 83.9 -0.25 -5.07 ZNF521 84.8 -0.24 -5.03 INPP1 69.0 -0.54 -4.79 LOX 70.7 -0.50 -4.75 UNC5B 63.0 -0.67 -4.53 HERC2 70.9 -0.50 -4.44 KREMEN1 81.6 -0.29 -4.43 RASA3 58.4 -0.78 -4.36 ITGA4 75.9 -0.40 -4.21 STK11 76.6 -0.39 -4.01 USP44 73.0 -0.46 -4.00 RASA2 81.9 -0.29 -3.98 MED13L 66.5 -0.59 -3.96 NYX 69.8 -0.52 -3.92 WDR83 77.1 -0.37 -3.91 USP31 87.8 -0.19 -3.88 BAAT 81.3 -0.30 -3.88
Supplementary Table S5 (continued) H2126 BrdU Assay
Normalised Fold change Gene SSMD mean (log2) NAP1L2 73.8 -0.44 -3.85 TMEM47 86.0 -0.22 -3.84 NXF3 65.6 -0.61 -3.72 TP53 77.5 -0.37 -3.70 PTCH2 78.2 -0.36 -3.62 ITIH5L 73.1 -0.45 -3.54 RAP2B 73.9 -0.44 -3.39 KLK10 66.3 -0.60 -3.39 FRK 68.4 -0.55 -3.37 PFKP 78.2 -0.35 -3.27 NUP54 80.8 -0.31 -3.27 CSMD3 63.1 -0.67 -3.25 FAM70A 79.4 -0.33 -3.24 TRIM37 78.8 -0.34 -3.19 KLHL10 81.2 -0.30 -3.14 F9 79.8 -0.33 -3.09 SGK3 57.5 -0.81 -3.04 CXorf57 82.9 -0.27 -3.03 BRE 80.3 -0.31 -3.02 PTPN9 75.3 -0.41 -2.98 TRPM6 88.2 -0.18 -2.97 SMYD1 78.6 -0.35 -2.95 RARA 71.2 -0.49 -2.93 VIM 78.6 -0.35 -2.87 TAF1L 87.9 -0.19 -2.71 BRCC3 64.0 -0.66 -2.70 PAX7 76.7 -0.38 -2.44 IKZF1 83.7 -0.26 -2.34 MCM3AP 72.9 -0.46 -2.29 ITGA9 84.7 -0.24 -2.24 CXorf30 81.2 -0.30 -2.20 KDR 86.5 -0.21 -2.05 ADAMTS20 81.0 -0.31 -2.03 ATR 91.4 -0.13 -2.02 NTRK3 79.7 -0.33 -1.85 KIF16B 85.3 -0.23 -1.84 IRS4 84.3 -0.25 -1.82 PIP4K2C 86.0 -0.22 -1.82 DDX51 83.3 -0.27 -1.81 RGS14 76.1 -0.40 -1.73
Supplementary Table S5 (continued) H2126 BrdU Assay
Normalised Fold change Gene SSMD mean (log2) TCEAL5 84.4 -0.25 -1.72 NHEJ1 84.9 -0.24 -1.72 TPR 80.8 -0.31 -1.56 PRKD2 83.6 -0.26 -1.56 CAMK2G 79.3 -0.34 -1.55 ALDOA 66.9 -0.62 -1.55 PLK2 91.7 -0.12 -1.50 DARC 90.1 -0.15 -1.47 PLXNB3 86.3 -0.21 -1.45 DET1 93.1 -0.10 -1.45 ADHFE1 82.1 -0.30 -1.43 CXorf15 84.7 -0.24 -1.40 ANK1 68.5 -0.60 -1.38 E2F3 86.9 -0.21 -1.32 TTN 73.3 -0.47 -1.31 EPHA3 79.3 -0.35 -1.29 INPP4B 93.9 -0.09 -1.18 CDKN2A 83.6 -0.27 -1.16 SMC2 85.8 -0.23 -0.99 DCN 92.7 -0.11 -0.94 TAOK1 86.4 -0.22 -0.89 PTPRO 84.6 -0.26 -0.87 DNAJB11 93.3 -0.11 -0.66 CALCR 94.2 -0.09 -0.56 C11orf92 94.8 -0.08 -0.50 NCAPD3 96.4 -0.06 -0.38 INTS6 97.0 -0.05 -0.35 NEG 100.3 0.01 0.17
Supplementary Table S6 H1395 BrdU Assay
Normalised Fold change Gene SSMD mean (log2) FBX025 83.9 -0.25 -2.88 KIF11 58.6 -0.80 -2.20 CENPC1 82.3 -0.29 -2.10 NLRP4 81.5 -0.30 -2.06 DOT1L 91.5 -0.13 -2.06 ERN1 80.4 -0.32 -1.72 BIRC6 83.7 -0.27 -1.51 MYST2 81.2 -0.31 -1.48 FLCN 81.8 -0.30 -1.30 NLRP1 85.6 -0.23 -1.28 GNB5 78.2 -0.38 -1.17 HMGN5 85.7 -0.23 -1.09 CXCR2 90.6 -0.14 -1.08 PPARGC1A 74.2 -0.47 -1.06 BRAF 87.2 -0.21 -1.02 MYO3B 92.6 -0.11 -1.00 SOS1 87.5 -0.20 -0.95 SERPINA2 83.8 -0.28 -0.79 PTPRT 87.5 -0.23 -0.58 GPC3 90.6 -0.16 -0.56 IMPDH2 92.2 -0.13 -0.51 SMURF2 91.5 -0.15 -0.45 PDCD5 88.7 -0.22 -0.45 PPIL5 91.4 -0.16 -0.45 MYCBP2 92.6 -0.13 -0.44 DDX23 91.0 -0.17 -0.44 RGAG1 93.2 -0.12 -0.42 NUP214 93.9 -0.11 -0.40 GHR 94.3 -0.11 -0.33 PROM1 94.2 -0.12 -0.30 STK11 98.4 -0.05 -0.14 ZC3H12B 99.3 -0.02 -0.09 ATM 99.4 -0.02 -0.08 NEG 100.0 0.00 -0.02 ! ! !
Supplementary Table S7 H1437 BrdU Assay
Normalised Fold change Gene SSMD mean (log2) POLG 65.1 -0.64 -10.24 ZNF608 57.9 -0.81 -7.18 KIF11 59.6 -0.77 -6.72 NIPBL 79.2 -0.36 -6.23 RAG1 67.2 -0.60 -6.18 KLK15 81.5 -0.31 -5.91 NTRK3 70.7 -0.52 -5.71 CDC5L 69.2 -0.55 -5.00 NCK2 74.8 -0.44 -4.93 GASP 77.6 -0.39 -4.74 ITM2A 84.3 -0.27 -4.66 EDD1 63.1 -0.69 -4.37 MOS 63.8 -0.67 -4.27 BIG1 81.8 -0.31 -4.15 PPPIR3A 83.3 -0.28 -3.95 FAM46D 75.0 -0.44 -3.93 PABPC5 74.9 -0.44 -3.68 NEK1 77.0 -0.40 -3.61 FBX040 86.0 -0.24 -3.28 CDKN2A 82.7 -0.29 -3.28 RGS6 66.2 -0.62 -3.23 PRKWNK2 70.8 -0.53 -3.23 NALP14 85.8 -0.24 -3.15 ITGA2B 68.8 -0.57 -3.10 IRAK2 62.5 -0.71 -3.07 DACT1 88.6 -0.20 -2.90 FASTK 80.6 -0.34 -2.83 HUS1 81.2 -0.32 -2.74 UBE20 86.8 -0.22 -2.73 LRGUK 80.2 -0.34 -2.71 PRKR 90.0 -0.17 -2.71 MAPK15 84.9 -0.26 -2.27 SENP5 85.4 -0.25 -2.22 TCF12 85.2 -0.25 -2.09 TRIM9 76.5 -0.41 -2.03 INTS2 84.9 -0.26 -2.02 FLJ44635 90.1 -0.17 -1.27 TP53 88.9 -0.20 -1.14
Supplementary Table S7 (continued) H1437 BrdU Assay
Normalised Fold change Gene SSMD mean (log2) GRIN2B 93.0 -0.13 -1.12 SERPINA12 89.1 -0.19 -1.09 SESN2 92.4 -0.14 -1.03 INCENP 92.3 -0.14 -1.03 NALP1 94.7 -0.10 -0.84 HDAC9 95.7 -0.09 -0.57 NEG 101.1 -0.01 -0.08
! !
Supplementary Table S8 H2087 Caspase 3/7 Assay
Normalised Fold change Gene SSMD mean (log2) ITGA10 166.8 0.73 5.52 6.67>β≥4.7 IKZF3 156.9 0.65 5.03 DGKD 166.0 0.73 4.70 FZR1 165.7 0.72 4.58 ITGA8 152.5 0.60 4.44 PAK5 179.7 0.84 4.37 MEFV 170.9 0.76 4.21 GATA2 163.3 0.70 4.15 NRAS 161.2 0.68 4.15 MCF2 164.3 0.71 4.13 GLP1R 151.4 0.59 4.03 LOC389904 120.5 0.27 4.02 DGKB 143.5 0.52 3.97 MEX3D 155.7 0.63 3.95 KIF11 195.4 0.95 3.94 HEPH 152.3 0.60 3.78 EPHA5 160.9 0.68 3.72 4.7>β≥3 FOXP2 175.4 0.80 3.70 BRAF 159.2 0.66 3.58 ORC6L 135.5 0.43 3.57 MAGEB6B 142.5 0.51 3.53 CSMD3 165.1 0.71 3.49 ITGA2 151.8 0.59 3.46 MYH11 141.1 0.49 3.44 HECW1 144.3 0.52 3.38 DOCK1 143.7 0.52 3.35 FBX046 146.2 0.54 3.33 AATK 134.6 0.42 3.24 ITGA6 157.1 0.64 3.19 GPR50 144.0 0.52 3.18 NCOA7 145.2 0.53 3.13 ZFYVE9 144.8 0.53 3.12 HERC6 135.1 0.43 3.11 NUP153 143.1 0.51 2.85 SERPINB4 135.2 0.43 2.80 KNTC1 133.1 0.41 2.69 ZNF630 128.0 0.35 2.51 ! HDAC7 126.4 0.33 2.43
Supplementary Table S8 (continued) H2087 Casase 3/7 Assay
Normalised Fold change Gene SSMD mean (log2) ERB2IP 135.1 0.43 2.40 FBX034 124.0 0.31 2.33 SGK2 132.5 0.40 2.31 NTRK3 124.0 0.31 2.29 TGS1 127.5 0.34 2.26 ADH7 143.5 0.51 2.24 PTPRT 126.1 0.33 2.22 SERPINA13 128.3 0.35 2.20 NLRP13 118.8 0.24 2.18 ITPR2 127.2 0.34 2.13 ADAMTSL3 127.9 0.35 2.06 NOS1AP 131.6 0.39 1.98 ULK1 125.7 0.32 1.97 NLRP4 125.3 0.32 1.89 LHFP 130.5 0.37 1.82 RNF130 116.5 0.22 1.82 TPR 116.6 0.22 1.78 TP53 117.1 0.22 1.77 PPP1R10 111.3 0.15 1.66 KSR2 116.3 0.21 1.63 FBLN5 117.4 0.23 1.49 CRAF 116.9 0.22 1.43 TEC 116.5 0.21 1.39 ELK5 115.8 0.21 1.37 PLS3 122.4 0.28 1.36 ATM 107.7 0.11 1.15 SGMS2 107.8 0.11 1.00 SHARP 108.0 0.11 0.92 SLK 106.9 0.09 0.91 CCNT1 108.8 0.12 0.85 CHD1L 104.7 0.06 0.59 LRGUK 102.3 0.03 0.18 NEG 100.5 0.01 0.08 CCNG2 98.7 -0.02 -0.22 NOTCH4 97.6 -0.04 -0.34 BAI3 93.0 -0.11 -0.95
! ! Supplementary Table 9 ON-TARGETplus SMARTpool siRNA library NCI-H1395 Duplex Pool Catalog Gene GI Catalog Gene Symbol GENE ID Sequence Number Accession Number Number L-003201-00 J-003201-11 ATM 472 NM_138292 73486662 GCAAAGCCCUAGUAACAUA L-003201-00 J-003201-12 ATM 472 NM_138292 73486662 GGUGUGAUCUUCAGUAUAU L-003201-00 J-003201-13 ATM 472 NM_138292 73486662 GAGAGGAGACAGCUUGUUA L-003201-00 J-003201-14 ATM 472 NM_138292 73486662 GAUGGGAGGCCUAGGAUUU N/A TROWA-000019 BRAF 673 NM_004333 187608632 AAAGAAUUGGAUCUGGAUCAUUU L-013857-00 J-013857-05 BIRC6 57448 NM_016252 61744455 ACAAGCACCUCUCGCAUUA L-013857-00 J-013857-06 BIRC6 57448 NM_016252 61744455 GGUCAAAGAUCACUUAGUA L-013857-00 J-013857-07 BIRC6 57448 NM_016252 61744455 GCAACGAUGUGCCAUGUUA L-013857-00 J-013857-08 BIRC6 57448 NM_016252 61744455 CAAUAGAUCUGACUGUUAA L-003251-00 J-003251-11 CENPC1 1060 NM_001812 68508960 GCGAAUAGAUUAUCAAGGA L-003251-00 J-003251-12 CENPC1 1060 NM_001812 68508960 CCAUAAACCUCACCCAGUA L-003251-00 J-003251-13 CENPC1 1060 NM_001812 68508960 UUUGAUAGGUGAAACAGUA L-003251-00 J-003251-14 CENPC1 1060 NM_001812 68508960 CGAAGUUGAUAGAGGAUGA L-005647-00 J-005647-05 IL8RB/CXCR2 3579 NM_001557 29171680 UCUAAGACCUCCUGCCUAA L-005647-00 J-005647-06 IL8RB/CXCR2 3579 NM_001557 29171680 GAAGGACCGUCUACUCAUC L-005647-00 J-005647-07 IL8RB/CXCR2 3579 NM_001557 29171680 CCUCAAGAUUCUAGCUAUA L-005647-00 J-005647-08 IL8RB/CXCR2 3579 NM_001557 29171680 GAGGACAUGGGCAACAAUA L-019861-01 J-019861-09 DDX23 9416 NM_004818 41327770 GGACAAGGACCGUAAACGA L-019861-01 J-019861-10 DDX23 9416 NM_004818 41327770 CAGCUUAUCUCCUGGUCGA L-019861-01 J-019861-11 DDX23 9416 NM_004818 41327770 GCACAAAGAACGAGAACGA L-019861-01 J-019861-12 DDX23 9416 NM_004818 41327770 CCAACUUUGAGUCGGGAAA
NCI-H1395 (continued) L-014900-01 J-014900-09 DOT1L 84444 NM_032482 22094134 UCACUAUGGCGUCGAGAAA L-014900-01 J-014900-10 DOT1L 84444 NM_032482 22094134 GCUAUGGAGAAUUACGUUU L-014900-01 J-014900-11 DOT1L 84444 NM_032482 22094134 GCAGAAUCGUGUCCUCGAA L-014900-01 J-014900-12 DOT1L 84444 NM_032482 22094134 AAGAGUGGAGGGAGCGAAU L-004951-02 J-004951-19 ERN1 2081 NM_001433 153946420 CUACCCAAACAUCGGGAAA L-004951-02 J-004951-20 ERN1 2081 NM_001433 153946420 CUCCAGAGAUGCUGAGCGA L-004951-02 J-004951-21 ERN1 2081 NM_001433 153946420 AUAAUGAAGGCCUGACGAA L-004951-02 J-004951-22 ERN1 2081 NM_001433 153946420 GUCCAGCUGUUGCGAGAAU L-019192-00 J-019192-05 FBXO25 26260 NM_012173 34878756 CGUUGUAUAUGCUUAGUGA L-019192-00 J-019192-06 FBXO25 26260 NM_012173 34878756 UAUUUGGAUUUGCCGAUUA L-019192-00 J-019192-07 FBXO25 26260 NM_012173 34878756 CCAGUUAACUUCAUUGAGU L-019192-00 J-019192-08 FBXO25 26260 NM_012173 34878756 AAUCCUCGCUUAAUCAAAG L-009998-00 J-009998-05 BHD/FLCN 201163 NM_144606 51317378 AAAGAGACCUCCAUUAAAU L-009998-00 J-009998-06 BHD/FLCN 201163 NM_144606 51317378 CGGGAUAUAUCAGCCAUGA L-009998-00 J-009998-07 BHD/FLCN 201163 NM_144606 51317378 ACACAGCCUUCACGCCAUU L-009998-00 J-009998-08 BHD/FLCN 201163 NM_144606 51317378 GAGGAUACCUUGGUCCAGA L-010402-00 J-010402-05 GHR 2690 NM_000163 58530854 GACUAUACCUCCAUUCAUA L-010402-00 J-010402-06 GHR 2690 NM_000163 58530854 GAUGGAUGGUUCUGGAGUA L-010402-00 J-010402-07 GHR 2690 NM_000163 58530854 UCAGUGAGGUGCUCUAUGU L-010402-00 J-010402-08 GHR 2690 NM_000163 58530854 CAAGGUUGAAUCACACAUA L-017235-00 J-017235-05 GNB5 10681 NM_006578 61835190 CUAAUCAUCUUCUGACAGU L-017235-00 J-017235-06 GNB5 10681 NM_006578 61835190 UAAUAAGUGUUCUGUGUAC L-017235-00 J-017235-07 GNB5 10681 NM_006578 61835190 AUAGGGAGGUUGCCAUCUA L-017235-00 J-017235-08 GNB5 10681 NM_006578 61835190 CUACGUGUCGCCUCUAUGA
NCI-H1395 (continued) L-017235-00 J-017235-08 GNB5 10681 NM_006578 61835190 CUACGUGUCGCCUCUAUGA L-011868-00 J-011868-05 GPC3 2719 NM_004484 5360213 UGGUGGAGAUUGACAAGUA L-011868-00 J-011868-06 GPC3 2719 NM_004484 5360213 AAGGUAGAGUUCUGGAUAA L-011868-00 J-011868-07 GPC3 2719 NM_004484 5360213 GAGCAAGACGUGACCUGAA L-011868-00 J-011868-08 GPC3 2719 NM_004484 5360213 GGAUCAGAUUUGCAAGUAU L-014649-01 J-014649-09 NSBP1/HMGN5 79366 NM_030763 13540522 GCCACAGAGUAUUGUUUAA L-014649-01 J-014649-10 NSBP1/HMGN5 79366 NM_030763 13540522 CAUAGAUACAAGUGCCCAA L-014649-01 J-014649-11 NSBP1/HMGN5 79366 NM_030763 13540522 CCUUCAUGUUGUAAAGUUA L-014649-01 J-014649-12 NSBP1/HMGN5 79366 NM_030763 13540522 AUUCAAUGGUUGUCAGUAU L-004330-00 J-004330-06 IMPDH2 3615 NM_000884 66933015 GGAAAGUUGCCCAUUGUAA L-004330-00 J-004330-07 IMPDH2 3615 NM_000884 66933015 GCACGGCGCUUUGGUGUUC L-004330-00 J-004330-08 IMPDH2 3615 NM_000884 66933015 AAGGGUCAAUCCACAAAUU L-004330-00 J-004330-09 IMPDH2 3615 NM_000884 66933015 GGUAUGGGUUCUCUCGAUG L-006951-00 J-006951-05 MYCBP2 23077 NM_015057 56118211 GGAAGUAGUUCUGUUAUUU L-006951-00 J-006951-06 MYCBP2 23077 NM_015057 56118211 GCAGAAAUGCCCACACUUU L-006951-00 J-006951-07 MYCBP2 23077 NM_015057 56118211 GAAUAGUUGUGGUUGGUUU L-006951-00 J-006951-08 MYCBP2 23077 NM_015057 56118211 GCAAGGAUUUGGUGUGUGU L-004863-00 J-004863-05 MYO3B 140469 NM_138995 20428778 GGGCAAGAAUCUCUGAAUA L-004863-00 J-004863-06 MYO3B 140469 NM_138995 20428778 ACAAAGAGGUGCACUCAGU L-004863-00 J-004863-07 MYO3B 140469 NM_138995 20428778 GAUAAUCUACGAUGCAAAU L-004863-00 J-004863-08 MYO3B 140469 NM_138995 20428778 GAAACGUUAUGCAGACUUG L-017668-00 J-017668-05 MYST2 11143 NM_007067 34222309 GAACCGAAGAUUCCGAUUU L-017668-00 J-017668-06 MYST2 11143 NM_007067 34222309 GAGGGAAGCAACAUGAUUA L-017668-00 J-017668-07 MYST2 11143 NM_007067 34222309 UAGGACACCUUACAGGAAA L-017668-00 J-017668-08 MYST2 11143 NM_007067 34222309 GGCAAGAUGCUUAUUGAUU
NCI-H1395 (continued) L-017668-00 J-017668-08 MYST2 11143 NM_007067 34222309 GGCAAGAUGCUUAUUGAUU L-004423-00 J-004423-06 NALP1/NLPR1 22861 NM_001033053 74271813 GCUAGAAGCAUAUGGAAUA L-004423-00 J-004423-07 NALP1/NLPR1 22861 NM_001033053 74271813 CCAAAUGGCCCACUUUAAA L-004423-00 J-004423-08 NALP1/NLPR1 22861 NM_001033053 74271813 GAUGAAAGGCAAGCAAUUA L-004423-00 J-004423-09 NALP1/NLPR1 22861 NM_001033053 74271813 GAGCUGGACCUAAGUGGAA L-015351-01 J-015351-09 NALP4/NLRP4 147945 NM_134444 54792746 GCUCUGACGCAUACGGAUU L-015351-01 J-015351-10 NALP4/NLRP4 147945 NM_134444 54792746 CUGAAAUGCUUCUGCGUAA L-015351-01 J-015351-11 NALP4/NLRP4 147945 NM_134444 54792746 ACAACAAGAAGCUGACGUA L-015351-01 J-015351-12 NALP4/NLRP4 147945 NM_134444 54792746 CCUAUCAAGCUCACGCAAA L-011980-00 J-011980-05 NUP214 8021 NM_005085 24497442 UCAAAUACCUCUAACCUAU L-011980-00 J-011980-06 NUP214 8021 NM_005085 24497442 GCAACACCCUCCACUAAAG L-011980-00 J-011980-07 NUP214 8021 NM_005085 24497442 GAAAUCGUGACUCUGGUUA L-011980-00 J-011980-08 NUP214 8021 NM_005085 24497442 CCACAAGCCUAACUAGUAC L-004439-00 J-004439-06 PDCD5 9141 NM_004708 21735599 CUAAAGCAGUAGAGAAUUA L-004439-00 J-004439-07 PDCD5 9141 NM_004708 21735599 GGCAAGAUAUGGACAACUA L-004439-00 J-004439-08 PDCD5 9141 NM_004708 21735599 GCAGAAAUGAGAAACAGUA L-004439-00 J-004439-09 PDCD5 9141 NM_004708 21735599 GAUGACGAUUAUUGAACUA L-005111-00 J-005111-05 PPARGC1A 10891 NM_013261 29570796 GAGAAUUCAUGGAGCAAUA L-005111-00 J-005111-06 PPARGC1A 10891 NM_013261 29570796 GAAGAGCGCCGUGUGAUUU L-005111-00 J-005111-07 PPARGC1A 10891 NM_013261 29570796 ACACUCAGCUAAGUUAUAA L-005111-00 J-005111-08 PPARGC1A 10891 NM_013261 29570796 GCAGGUAACAUGUUCCCUA L-016820-01 J-016820-09 PPIL5 122769 NM_203467 45439345 GAAAGCCACUGUUCGGUUA L-016820-01 J-016820-10 PPIL5 122769 NM_203467 45439345 GUGGAUAUCUGUCUAAGUA L-016820-01 J-016820-11 PPIL5 122769 NM_203467 45439345 ACACUGUGGUCUUAGUAGA L-016820-01 J-016820-12 PPIL5 122769 NM_203467 45439345 GCGUCAAAUUGGAGUAAGG
NCI-H1395 (continued) L-010630-00 J-010630-07 PROM1 8842 NM_006017 5174386 GCUAAGUACUAUCGUCGAA L-010630-00 J-010630-08 PROM1 8842 NM_006017 5174386 GAACAAGUUUACAGUGACU L-010630-00 J-010630-09 PROM1 8842 NM_006017 5174386 GAAGUAUGGGAGAACAAUA L-010630-00 J-010630-10 PROM1 8842 NM_006017 5174386 UCACAAUCCUGUUAUGACA L-008072-00 J-008072-06 PTPRT 11122 NM_007050 48762923 GGAGGCUGGUGUUCGAUUA L-008072-00 J-008072-07 PTPRT 11122 NM_007050 48762923 GGUCUGGUGUGUCCAACUA L-008072-00 J-008072-08 PTPRT 11122 NM_007050 48762923 CCAACUACAUUGACGGAUA L-008072-00 J-008072-09 PTPRT 11122 NM_007050 48762923 GAAGAUGGCUGGCGUGAUC L-014024-02 J-014024-17 KIAA1318/RGAG1 57529 NM_020769 24308214 GGAAUAAUGUCAUCGCUUU L-014024-02 J-014024-18 KIAA1318/RGAG1 57529 NM_020769 24308214 GUUGCGAACUCUAGAUCUA L-014024-02 J-014024-19 KIAA1318/RGAG1 57529 NM_020769 24308214 GAGCAGACGUACAGAUUCU L-014024-02 J-014024-20 KIAA1318/RGAG1 57529 NM_020769 24308214 AGUGAUAGUAUAAUGGCUA L-030366-01 J-030366-09 LOC390502/SERPINA2 390502 XM_372532 89037491 AAUUCAAGGCUGAGCGCAU L-030366-01 J-030366-10 LOC390502/SERPINA2 390502 XM_372532 89037491 AGGCCAAGAUCCACGAAUG L-030366-01 J-030366-11 LOC390502/SERPINA2 390502 XM_372532 89037491 CCAGAGAGCCUUUGACAUA L-030366-01 J-030366-12 LOC390502/SERPINA2 390502 XM_372532 89037491 GUAUGAAGCUAGUGGACAC L-007194-00 J-007194-05 SMURF2 64750 NM_022739 56550041 GUUAAUGACUGGAAGGUAA L-007194-00 J-007194-06 SMURF2 64750 NM_022739 56550041 AGAAUACGCUUGAUCCAAA L-007194-00 J-007194-07 SMURF2 64750 NM_022739 56550041 CCACUUUGUUGGACGAAUA L-007194-00 J-007194-08 SMURF2 64750 NM_022739 56550041 GCAAAAGUAUCCCUGUUAA
NCI-H1395 (continued) L-005194-00 J-005194-06 SOS1 6654 NM_005633 15529995 AACAGAAGCUGAUCGCAUA L-005194-00 J-005194-07 SOS1 6654 NM_005633 15529995 GGCAGAAAUUCGACAAUAU L-005194-00 J-005194-08 SOS1 6654 NM_005633 15529995 GGAUAUGUUUCAUCAAGAU L-005194-00 J-005194-09 SOS1 6654 NM_005633 15529995 UAGUAGCAGUCUUAGAAUA L-005035-00 J-005035-07 STK11 6794 NM_000455 58530881 UGACUGUGGUGCCGUACUU L-005035-00 J-005035-08 STK11 6794 NM_000455 58530881 GCUCUUACGGCAAGGUGAA L-005035-00 J-005035-09 STK11 6794 NM_000455 58530881 UGAAAGGGAUGCUUGAGUA L-005035-00 J-005035-10 STK11 6794 NM_000455 58530881 GAAGAAGGAAAUUCAACUA L-024828-02 J-024828-18 LOC340554/ZC3H12B 340554 NM_001010888 148746186 GGAUUGGUUUCUAGAUAAA L-024828-02 J-024828-19 LOC340554/ZC3H12B 340554 NM_001010888 148746186 AGAUGGAGUUUGCGCUAAA L-024828-02 J-024828-20 LOC340554/ZC3H12B 340554 NM_001010888 148746186 GUUCCUAGUUAUUAGAGAA L-024828-02 J-024828-21 LOC340554/ZC3H12B 340554 NM_001010888 148746186 GCUUAGGGCUGCACGUUGA
ON-TARGETplus SMARTpool siRNA library NCI-H1437
Duplex Pool Catalog Gene GI Catalog Gene Symbol GENE ID Sequence Number Accession Number Number L-012207-00 J-012207-05 BIG1/ARFGEF1 10565 NM_006421 51479144 GUCCAAAUGUCCUCGCAUA L-012207-00 J-012207-06 BIG1/ARFGEF1 10565 NM_006421 51479144 GAACAGGUCUCCAACAAUU L-012207-00 J-012207-07 BIG1/ARFGEF1 10565 NM_006421 51479144 GAUCACAAAUGGAUGGUUA L-012207-00 J-012207-08 BIG1/ARFGEF1 10565 NM_006421 51479144 GAUGAUAGAUUGUCAGUCU L-011237-00 J-011237-05 CDC5L 988 NM_001253 16357499 CGAGACAAGUUAAACAUUA L-011237-00 J-011237-06 CDC5L 988 NM_001253 16357499 GAGAGGAGUUGAUUAUAAU L-011237-00 J-011237-07 CDC5L 988 NM_001253 16357499 CGAGGAAUCUGGCAUAACA L-011237-00 J-011237-08 CDC5L 988 NM_001253 16357499 GCUCUCAAGUGAAGCUUAU L-011007-00 J-011007-08 CDKN2A 1029 NM_058195 47132605 GAUCAUCAGUCACCGAAGG L-011007-00 J-011007-09 CDKN2A 1029 NM_058195 47132605 AAACACCGCUUCUGCCUUU L-011007-00 J-011007-10 CDKN2A 1029 NM_058195 47132605 UAACGUAGAUAUAUGCCUU L-011007-00 J-011007-11 CDKN2A 1029 NM_058195 47132605 CAGAACCAAAGCUCAAAUA L-016440-00 J-016440-05 DACT1 51339 NM_016651 38569506 AAACGGGAAUGAUGUAUAU L-016440-00 J-016440-06 DACT1 51339 NM_016651 38569506 GAACAUGGGCGUCGUGAAG L-016440-00 J-016440-07 DACT1 51339 NM_016651 38569506 AAGAGAUGCUGGUUUGUUG L-016440-00 J-016440-08 DACT1 51339 NM_016651 38569506 UGACUUGGAUACAAAUAAG L-003527-00 J-003527-09 PRKR/EIF2AK2 5610 NM_002759 4506102 GUAAGGGAACUUUGCGAUA L-003527-00 J-003527-10 PRKR/EIF2AK2 5610 NM_002759 4506102 GCGAGAAACUAGACAAAGU L-003527-00 J-003527-11 PRKR/EIF2AK2 5610 NM_002759 4506102 CGACCUAACACAUCUGAAA L-003527-00 J-003527-12 PRKR/EIF2AK2 5610 NM_002759 4506102 CCACAUGAUAGGAGGUUUA
NCI-H1437 (continued)
L-016938-02 J-016938-17 MGC26999/FAM46D 169966 NM_152630 141802763 GAGGAGCUGUCUUAAAUUA L-016938-02 J-016938-18 MGC26999/FAM46D 169966 NM_152630 141802763 GCUAUAAGGAUCUGGACGU L-016938-02 J-016938-19 MGC26999/FAM46D 169966 NM_152630 141802763 CAUGAAAGACGCUUACGUA L-016938-02 J-016938-20 MGC26999/FAM46D 169966 NM_152630 141802763 CGUUAUAUGUGCUCUAGAU L-005317-00 J-005317-07 FASTK 10922 NM_033015 39995106 GAACAGCAGUUUAUGCCCU L-005317-00 J-005317-08 FASTK 10922 NM_033015 39995106 GCAAGGUGGUACAGAAGUU L-005317-00 J-005317-09 FASTK 10922 NM_033015 39995106 ACAAGGACAUAGUAGCUGA L-005317-00 J-005317-10 FASTK 10922 NM_033015 39995106 UCAGAGCCCUGCACUUUGU L-020905-00 J-020905-05 FBXO40 51725 NM_016298 7706492 GAGAUGCCAUGUUGAGUUG L-020905-00 J-020905-06 FBXO40 51725 NM_016298 7706492 CAAAGGACUAUAACAUGUA L-020905-00 J-020905-07 FBXO40 51725 NM_016298 7706492 UGGAACCGCUGGCCAAAUG L-020905-00 J-020905-08 FBXO40 51725 NM_016298 7706492 AAAGAGGUAUUCCCAUGGA L-032096-02 J-032096-13 FLJ44635 392490 NM_207422 46409467 ACGAAGAAUGAGGGAGAAU L-032096-02 J-032096-14 FLJ44635 392490 NM_207422 46409467 AGGUAAUAGAUAUGUGCAU L-032096-02 J-032096-15 FLJ44635 392490 NM_207422 46409467 CUACAAUAAGUGCAUCAAA L-032096-02 J-032096-16 FLJ44635 392490 NM_207422 46409467 GCUCUGGUUUCAAGGAUGU L-021155-00 J-021155-05 GASP/GPRASP1 9737 NM_014710 62909991 GGUGUCAGAUGCAGAUUUA L-021155-00 J-021155-06 GASP/GPRASP1 9737 NM_014710 62909991 AAGAAUAAGUCCAAGGUUA L-021155-00 J-021155-07 GASP/GPRASP1 9737 NM_014710 62909991 GAGGAGACCAUAAUCAAUU L-021155-00 J-021155-08 GASP/GPRASP1 9737 NM_014710 62909991 GGUCAUUGGUCCCUGGUUU
NCI-H1437 (continued)
L-006196-00 J-006196-05 GRIN2B 2904 NM_000834 6006003 GAUCGGAGGUGGUCGACUU L-006196-00 J-006196-06 GRIN2B 2904 NM_000834 6006003 CUAAAUAGGUAUCUGAUCA L-006196-00 J-006196-07 GRIN2B 2904 NM_000834 6006003 CCUCAGAGCCCGACUAAUU L-006196-00 J-006196-08 GRIN2B 2904 NM_000834 6006003 GGGUGUAGAUGAUGCAUUG L-005241-00 J-005241-12 HDAC9 9734 NM_058177 17158040 UCUUGUAGCUGGUGGAGUU L-005241-00 J-005241-13 HDAC9 9734 NM_058177 17158040 GAGAAUUUCACCUGGCAUU L-005241-00 J-005241-14 HDAC9 9734 NM_058177 17158040 GAAGGUGCGGUCCAGGUUA L-005241-00 J-005241-15 HDAC9 9734 NM_058177 17158040 CAACUGGAAGUGUUACUGA L-003267-00 J-003267-09 HUS1 3364 NM_004507 31077213 ACAAGUAAAUCCCACAAAG L-003267-00 J-003267-10 HUS1 3364 NM_004507 31077213 UGAAGUGCACAUAGAUAUU L-003267-00 J-003267-11 HUS1 3364 NM_004507 31077213 CCAUAAAGGUGAUUCCUAG L-003267-00 J-003267-12 HUS1 3364 NM_004507 31077213 UCAGUAACAUGAUAGCCAA L-006823-00 J-006823-05 INCENP 3619 NM_020238 9910375 GUCAUGAAGUCCUUUAUUA L-006823-00 J-006823-06 INCENP 3619 NM_020238 9910375 GGAUGGAUCUGAAUAGCGA L-006823-00 J-006823-07 INCENP 3619 NM_020238 9910375 CAACGGAAAUAACUCGUGG L-006823-00 J-006823-08 INCENP 3619 NM_020238 9910375 CGGAAGAAGAGACGGAUUU L-013999-02 J-013999-17 KIAA1287/INTS2 57508 NM_020748 24308210 CAAUAAAGUUUGUACGACA L-013999-02 J-013999-18 KIAA1287/INTS2 57508 NM_020748 24308210 GAUAAAAGAAGAAGCGUAU L-013999-02 J-013999-19 KIAA1287/INTS2 57508 NM_020748 24308210 UAACACAGGCUAAGCGGUA L-013999-02 J-013999-20 KIAA1287/INTS2 57508 NM_020748 24308210 UCUUGGAACAAAUGCGAAA
NCI-H1437 (continued)
L-004761-00 J-004761-05 IRAK2 3656 NM_001570 58530885 CAAGACAGUUUCACAGCUU L-004761-00 J-004761-06 IRAK2 3656 NM_001570 58530885 GAAACUUCGUGGCAAAUUG L-004761-00 J-004761-07 IRAK2 3656 NM_001570 58530885 GAAGCCCGGUUUACCUGAA L-004761-00 J-004761-08 IRAK2 3656 NM_001570 58530885 CAAAGCGAGUGGACAUCUU L-008001-00 J-008001-09 ITGA2B 3674 NM_000419 6006009 AGAUAGGAAUCGCGAUGUU L-008001-00 J-008001-10 ITGA2B 3674 NM_000419 6006009 GAUGAGACCCGAAAUGUAG L-008001-00 J-008001-11 ITGA2B 3674 NM_000419 6006009 GCCCGUAGGUAGCUGCUUU L-008001-00 J-008001-12 ITGA2B 3674 NM_000419 6006009 GCACACAGCUCUAUGGGCG L-019433-02 J-019433-19 ITM2A 9452 NM_004867 74316000 CUGUAAUUAACCUGACUUA L-019433-02 J-019433-20 ITM2A 9452 NM_004867 74316000 GAGAAGACCUAGUUGCUGU L-019433-02 J-019433-21 ITM2A 9452 NM_004867 74316000 GUCAAGAGUAAGAGGCAAC L-019433-02 J-019433-22 ITM2A 9452 NM_004867 74316000 CGGCAAGGAGCUCCGAGUU L-005912-00 J-005912-05 KLK15 55554 NM_138564 20302146 GCAAGUGGCUCUCUACGAG L-005912-00 J-005912-06 KLK15 55554 NM_138564 20302146 GAAGAGGAACUGACUAUUC L-005912-00 J-005912-07 KLK15 55554 NM_138564 20302146 GUGACAAGUUGCUGGAAGG L-005912-00 J-005912-08 KLK15 55554 NM_138564 20302146 CGUGGACGCUUUAACUGUG L-015997-01 J-015997-09 FLJ32786/LRGUK 136332 NM_144648 21389482 GUUACAGUUUCGCGCAGAA L-015997-01 J-015997-10 FLJ32786/LRGUK 136332 NM_144648 21389482 CGUAGAAGGUAUCGCAAGA L-015997-01 J-015997-11 FLJ32786/LRGUK 136332 NM_144648 21389482 ACAAUAAGAUCACGACAAU L-015997-01 J-015997-12 FLJ32786/LRGUK 136332 NM_144648 21389482 GAGGGAUAUUUGCGGAGAA
NCI-H1437 (continued)
L-004807-00 J-004807-06 ERK8/MAPK15 225689 NM_139021 20502985 GUAGUGGACCCUCGCAUUG L-004807-00 J-004807-07 ERK8/MAPK15 225689 NM_139021 20502985 GACCAGAAGCCGUCCAAUG L-004807-00 J-004807-08 ERK8/MAPK15 225689 NM_139021 20502985 GGAGAUACCUACUCAGGCG L-004807-00 J-004807-09 ERK8/MAPK15 225689 NM_139021 20502985 GAACGACAGGGACAUUUAC L-003859-00 J-003859-08 MOS 4342 NM_005372 4885488 CAACGUCACUUUACACCAA L-003859-00 J-003859-09 MOS 4342 NM_005372 4885488 CGGUGUACAAGGCGACUUA L-003859-00 J-003859-10 MOS 4342 NM_005372 4885488 GCAAGGCUGCGCCACGAUA L-003859-00 J-003859-11 MOS 4342 NM_005372 4885488 GCGAACAUCUUGAUCAGUG L-019547-00 J-019547-05 NCK2 8440 NM_001004722 52630426 CUUAAAGCGUCAGGGAAGA L-019547-00 J-019547-06 NCK2 8440 NM_001004722 52630426 CCAACUACGUGGAGCGGAA L-019547-00 J-019547-07 NCK2 8440 NM_001004722 52630426 CAACAGGACGGGCUAUGUA L-019547-00 J-019547-08 NCK2 8440 NM_001004722 52630426 GAAGUUAUUGUGAUAGCCA L-004864-00 J-004864-05 NEK1 4750 NM_012224 41872672 GGACUGGUUUGUACAGAUA L-004864-00 J-004864-06 NEK1 4750 NM_012224 41872672 GGAGAAGUAUGUUAGACUA L-004864-00 J-004864-07 NEK1 4750 NM_012224 41872672 GAAACUCAGUCGGCAGAUA L-004864-00 J-004864-08 NEK1 4750 NM_012224 41872672 GAGAAAUACUUCGUAGAUU L-012980-00 J-012980-07 NIPBL 25836 NM_015384 47578106 GAUAUAAACCGCCCACUAA L-012980-00 J-012980-08 NIPBL 25836 NM_015384 47578106 CAACAGAUCACAUAGAGUU L-012980-00 J-012980-09 NIPBL 25836 NM_015384 47578106 ACACUUCACUUCUAACAAA L-012980-00 J-012980-10 NIPBL 25836 NM_015384 47578106 GGGCUUGUUUCAAUAGAUA
NCI-H1437 (continued)
L-004423-00 J-004423-06 NALP1/NLRP1 22861 NM_001033053 74271813 GCUAGAAGCAUAUGGAAUA L-004423-00 J-004423-07 NALP1/NLRP1 22861 NM_001033053 74271813 CCAAAUGGCCCACUUUAAA L-004423-00 J-004423-08 NALP1/NLRP1 22861 NM_001033053 74271813 GAUGAAAGGCAAGCAAUUA L-004423-00 J-004423-09 NALP1/NLRP1 22861 NM_001033053 74271813 GAGCUGGACCUAAGUGGAA L-019075-00 J-019075-05 NALP14/NLRP14 338323 NM_176822 31543282 GAAUAUGGACUAUGACUUA L-019075-00 J-019075-06 NALP14/NLRP14 338323 NM_176822 31543282 GAUAAGAGGUGGGCCAUGA L-019075-00 J-019075-07 NALP14/NLRP14 338323 NM_176822 31543282 GAAUGAAGAUCGAGUAAAA L-019075-00 J-019075-08 NALP14/NLRP14 338323 NM_176822 31543282 GAAUUGGACCUGUACCAUA L-003161-00 J-003161-09 NTRK3 4916 NM_002530 59889559 UGUAGUUUCUGGCGGAUUU L-003161-00 J-003161-10 NTRK3 4916 NM_002530 59889559 ACGGACAUCUCAAGGAAUA L-003161-00 J-003161-11 NTRK3 4916 NM_002530 59889559 GGUCGACGGUCCAAAUUUG L-003161-00 J-003161-12 NTRK3 4916 NM_002530 59889559 GCAAGACUGAGAUCAAUUG L-015277-02 J-015277-17 PABPC5 140886 NM_080832 211904087 AAACAUUGGAGACGACAUA L-015277-02 J-015277-18 PABPC5 140886 NM_080832 211904087 CUGCAAAGUCGUAUGCGAU L-015277-02 J-015277-19 PABPC5 140886 NM_080832 211904087 GAUGAAUAACUGCGCUGAA L-015277-02 J-015277-20 PABPC5 140886 NM_080832 211904087 GUUCAAUAUUGCCGUUACA L-012649-00 J-012649-05 POLG 5428 NM_002693 4505936 GGUAUCGGCUGUCGGAUGA L-012649-00 J-012649-06 POLG 5428 NM_002693 4505936 AGUGGGACCUGCAAGAAUU L-012649-00 J-012649-07 POLG 5428 NM_002693 4505936 GCUUACUAAUGCAGUUUAA L-012649-00 J-012649-08 POLG 5428 NM_002693 4505936 UCAGUGCAGUCGAUAUUGA
NCI-H1437 (continued)
L-010532-00 J-010532-06 PPP1R3A 5506 NM_002711 34304342 GAACUAGUGCAAUUACAUA L-010532-00 J-010532-07 PPP1R3A 5506 NM_002711 34304342 GAGAUAAUACGAGGAGUUU L-010532-00 J-010532-08 PPP1R3A 5506 NM_002711 34304342 GAAUCACGUUUAGGUAUUU L-010532-00 J-010532-09 PPP1R3A 5506 NM_002711 34304342 CAGCAGAUGUGUCUCAUUC L-006549-00 J-006549-07 RAG1 5896 NM_000448 4557840 GGAUGCAGUUUGUGAGUUA L-006549-00 J-006549-08 RAG1 5896 NM_000448 4557840 GAAAUGAGUCUGGUAACAA L-006549-00 J-006549-09 RAG1 5896 NM_000448 4557840 CAGCUAAACCUUUCAUUGA L-006549-00 J-006549-10 RAG1 5896 NM_000448 4557840 GCAAUGCAGCUGAGUUCUA L-019082-00 J-019082-05 RGS6 9628 NM_004296 31742475 GGACCCAGUUGAAGCAAUA L-019082-00 J-019082-06 RGS6 9628 NM_004296 31742475 GAUGAAGAGUGACAGCUAU L-019082-00 J-019082-07 RGS6 9628 NM_004296 31742475 GGGCUUCUCUUUCGAUGAG L-019082-00 J-019082-08 RGS6 9628 NM_004296 31742475 GCGAUGACGUUGCUUUGUG L-005946-00 J-005946-05 SENP5 205564 NM_152699 34303948 GUACAGAGCUGAUUCAUGA L-005946-00 J-005946-06 SENP5 205564 NM_152699 34303948 GAGGAAAGGAAUCCACUUA L-005946-00 J-005946-07 SENP5 205564 NM_152699 34303948 UAACCAACAUAGAAGGAUA L-005946-00 J-005946-08 SENP5 205564 NM_152699 34303948 GGGCAAGGAGCUUAGUUUA L-008739-02 J-008739-18 SERPINA12 145264 NM_173850 31341191 AUUAACAACCUGAUCGAGA L-008739-02 J-008739-19 SERPINA12 145264 NM_173850 31341191 AGACACCACUCGUCGUCAA L-008739-02 J-008739-20 SERPINA12 145264 NM_173850 31341191 CAAGUUGGCUAUGACGAUA L-008739-02 J-008739-21 SERPINA12 145264 NM_173850 31341191 GGAUGAGAGGGGUACGGAA
NCI-H1437 (continued)
J-019134-17 SESN2 83667 NM_031459 32454742 GGAGGGAGUAUUAGAUUAU J-019134-18 SESN2 83667 NM_031459 32454742 GCAGGGACCCGUUGAACAA J-019134-19 SESN2 83667 NM_031459 32454742 GCGCAAACUCAGCGAGAUC J-019134-20 SESN2 83667 NM_031459 32454742 CUUUCGGAUAUGAGGACUU J-006356-07 TCF12 6938 NM_207040 46370085 UCGAUCAGCUUCAAUGGUU J-006356-08 TCF12 6938 NM_207040 46370085 GCAGUCAUCCUUAGUCUAG J-006356-09 TCF12 6938 NM_207040 46370085 UCACAUGACCGCUUGAGUU J-006356-10 TCF12 6938 NM_207040 46370085 GCACAGACCUGAACCAUAA J-003329-14 TP53 7157 NM_000546 8400737 GAAAUUUGCGUGUGGAGUA J-003329-15 TP53 7157 NM_000546 8400737 GUGCAGCUGUGGGUUGAUU J-003329-16 TP53 7157 NM_000546 8400737 GCAGUCAGAUCCUAGCGUC J-003329-17 TP53 7157 NM_000546 8400737 GGAGAAUAUUUCACCCUUC J-012974-05 TRIM9 114088 NM_052978 46370052 CAACGGCGUCCGCGUGUUU J-012974-06 TRIM9 114088 NM_052978 46370052 GCGCUACGUUGUCCUGGAA J-012974-07 TRIM9 114088 NM_052978 46370052 AAACAGGAGUCAGCCCGUA J-012974-08 TRIM9 114088 NM_052978 46370052 ACAGUGAAAUUGCGCCAGA J-008979-05 UBE2O 63893 NM_022066 33636749 GACUUUAGGUUCCGUACAA J-008979-06 UBE2O 63893 NM_022066 33636749 GUUGUAGAGUUGAAAGUUA J-008979-07 UBE2O 63893 NM_022066 33636749 CGACUCGGGUCUCUUCUUC J-008979-08 UBE2O 63893 NM_022066 33636749 GAACCAUACUACAACGAAG
NCI-H1437 (continued)
J-007189-06 EDD1/UBR5 51366 NM_015902 41352716 GCACUUAUAUACUGGAUUA J-007189-07 EDD1/UBR5 51366 NM_015902 41352716 GAUUGUAGGUUACUUAGAA J-007189-08 EDD1/UBR5 51366 NM_015902 41352716 GAUCAAUCCUAACUGAAUU J-007189-09 EDD1/UBR5 51366 NM_015902 41352716 GGUCGAAGAUGUGCUACUA J-005363-06 PRKWNK2/WNK2 65268 NM_006648 32455272 GGACGCACCCGAUGAAAUU J-005363-07 PRKWNK2/WNK2 65268 NM_006648 32455272 CGUAUAUGGUGGAGCAUGA J-005363-08 PRKWNK2/WNK2 65268 NM_006648 32455272 GCAAACCUCGUGCUCGAUU J-005363-09 PRKWNK2/WNK2 65268 NM_006648 32455272 CCACGAGAGUGACGUCAAG J-024395-17 ZNF608 57507 NM_020747 153945754 CAACAUGACGGCUGCGUUA J-024395-18 ZNF608 57507 NM_020747 153945754 GAGGAGAGCCACAGGCGAA J-024395-19 ZNF608 57507 NM_020747 153945754 CCUCAAUGCCAGCGGACGA J-024395-20 ZNF608 57507 NM_020747 153945754 GCAAAUGGACUGUCGGAGU
ON-TARGETplus SMARTpool siRNA library NCI-H2009
Duplex Pool Catalog Gene GI Catalog Gene Symbol GENE ID Sequence Number Accession Number Number L-010756-00 J-010756-07 ADAM32 203102 NM_145004 40316951 CCAAGUAGCGAAUCUAAAU L-010756-00 J-010756-08 ADAM32 203102 NM_145004 40316951 UCUGAUAGCAUGAUAGUAA L-010756-00 J-010756-09 ADAM32 203102 NM_145004 40316951 GAACUGUGGUAGGGAUAGA L-005766-00 J-005766-06 ADAMTS15 170689 NM_139055 21265057 GCGCGGACCUGGAACAUUA L-005766-00 J-005766-07 ADAMTS15 170689 NM_139055 21265057 CGACUGGACCCGGACAUUA L-005766-00 J-005766-08 ADAMTS15 170689 NM_139055 21265057 GCGGACGAGUCAAUGGUCA L-005766-00 J-005766-09 ADAMTS15 170689 NM_139055 21265057 GCAAGUACUGCGAGGGAGU L-005771-00 J-005771-06 ADAMTS2 9509 NM_021599 11038658 ACACGGGCCACGAUGAAUA L-005771-00 J-005771-07 ADAMTS2 9509 NM_021599 11038658 GAACGAGCAAUGCCGCUUU L-005771-00 J-005771-08 ADAMTS2 9509 NM_021599 11038658 GCAAGGCCGUGUGCAUGUG L-005771-00 J-005771-09 ADAMTS2 9509 NM_021599 11038658 GCUGCACCCUGAACCAUGA L-005772-00 J-005772-06 ADAMTS20 80070 NM_175851 41281881 CAAGCCACCUAGCGUAAUA L-005772-00 J-005772-07 ADAMTS20 80070 NM_175851 41281881 GCAAGGAACUGUUAUUGAA L-005772-00 J-005772-08 ADAMTS20 80070 NM_175851 41281881 CAAGAACUGUCCCGAGUGA L-005772-00 J-005772-09 ADAMTS20 80070 NM_175851 41281881 GUACAGCUAUUCUGGACAA L-003808-00 J-003808-09 ALOX15 246 NM_001140 40316936 CGAUACACCCUGGAAAUUA L-003808-00 J-003808-10 ALOX15 246 NM_001140 40316936 AAACUGGAAGGACGGGUUA L-003808-00 J-003808-11 ALOX15 246 NM_001140 40316936 GAAGGAAGUUGUACCGGUG L-003808-00 J-003808-12 ALOX15 246 NM_001140 40316936 CAUCCUAUCUUCAAGCUUA
NCI-H2009 (continued)
L-010992-00 J-010992-05 ANK1 286 NM_020481 70780362 GAGUAGGGCUAGUGAAUGU L-010992-00 J-010992-06 ANK1 286 NM_020481 70780362 GUUAGGGACUUAAGAAUCU L-010992-00 J-010992-07 ANK1 286 NM_020481 70780362 GAGUGAACUUGGUGCUCUA L-010992-00 J-010992-08 ANK1 286 NM_020481 70780362 UAUCAUAGUCCGUGUUCAA L-014405-00 J-014405-05 ANKHD1 54882 NM_017978 46519150 CAAAGAAAGUGCUCUAACA L-014405-00 J-014405-06 ANKHD1 54882 NM_017978 46519150 AGCAGGGUAUUAUGAAUUA L-014405-00 J-014405-07 ANKHD1 54882 NM_017978 46519150 UGUCCGAGGUUGAAUCAUU L-014405-00 J-014405-08 ANKHD1 54882 NM_017978 46519150 GAUGCACACUGCCUUAAUG L-003456-01 J-003456-13 APAF1 317 NM_181869 32483362 AGAUAAUGAUUCCUACGUA L-003456-01 J-003456-14 APAF1 317 NM_181869 32483362 AGUAAUGGGUCCUAAAUAU L-003456-01 J-003456-15 APAF1 317 NM_181869 32483362 GUAUGAUGGAGCUGCAAAU L-003456-01 J-003456-16 APAF1 317 NM_181869 32483362 UCAUGUAGGCAGAGUAUAA L-031158-00 J-031158-05 ASB15 142685 NM_080928 38261966 GUCAAGCGAUGGUCAGCAA L-031158-00 J-031158-06 ASB15 142685 NM_080928 38261966 AUAAGACACUCUGGGAAUU L-031158-00 J-031158-07 ASB15 142685 NM_080928 38261966 AGUCAAAGCUGGUCUGGUG L-031158-00 J-031158-08 ASB15 142685 NM_080928 38261966 GAUCAAACAUAACACUAGC L-019831-00 J-019831-05 ASH2L 9070 NM_004674 4757789 AAAGAUGGCUAUCGGUAUA L-019831-00 J-019831-06 ASH2L 9070 NM_004674 4757789 GGACCAUGCUUCAAGUAUC L-019831-00 J-019831-07 ASH2L 9070 NM_004674 4757789 GAGAAGGGCUACUCUAUGG L-019831-00 J-019831-08 ASH2L 9070 NM_004674 4757789 GAGACAGAAUCAUCUAAUG
NCI-H2009 (continued)
L-019289-00 J-019289-05 ATF7IP 55729 NM_018179 38261961 GAUAUAGCCUCUAGUGAAA L-019289-00 J-019289-06 ATF7IP 55729 NM_018179 38261961 GCUACCAUCUCUAUGCUUA L-019289-00 J-019289-07 ATF7IP 55729 NM_018179 38261961 AAAGAUGAGUCUGAAGUUA L-019289-00 J-019289-08 ATF7IP 55729 NM_018179 38261961 GUACAAGGUCAAAGAAGAA L-009787-00 J-009787-05 BACH2 60468 NM_021813 13540489 GUACCAAGAAUGUCUAUAA L-009787-00 J-009787-06 BACH2 60468 NM_021813 13540489 GCAAAUUGGUGUGUGAGAA L-009787-00 J-009787-07 BACH2 60468 NM_021813 13540489 GAAAGAUUAUACCUAGUGA L-009787-00 J-009787-08 BACH2 60468 NM_021813 13540489 GAGGAUGGCCUGUUUGUGU L-020290-00 J-020290-05 BCL2L13 23786 NM_015367 45243500 GUGAGAACAACUCCUCUAA L-020290-00 J-020290-06 BCL2L13 23786 NM_015367 45243500 UGACAUACCUGGAGGACUA L-020290-00 J-020290-07 BCL2L13 23786 NM_015367 45243500 CAGAAGUGAUCACAGUUGA L-020290-00 J-020290-08 BCL2L13 23786 NM_015367 45243500 GCUAAAAUCUCUGGACAAA L-013857-00 J-013857-05 BIRC6 57448 NM_016252 61744455 ACAAGCACCUCUCGCAUUA L-013857-00 J-013857-06 BIRC6 57448 NM_016252 61744455 GGUCAAAGAUCACUUAGUA L-013857-00 J-013857-07 BIRC6 57448 NM_016252 61744455 GCAACGAUGUGCCAUGUUA L-013857-00 J-013857-08 BIRC6 57448 NM_016252 61744455 CAAUAGAUCUGACUGUUAA L-005798-00 J-005798-12 CXORF53/BRCC3 79184 NM_001018055 64762483 CAUAAUGGCUCAGUGUUUA L-005798-00 J-005798-13 CXORF53/BRCC3 79184 NM_001018055 64762483 CGUCAGAAUUGUUCACAUU L-005798-00 J-005798-14 CXORF53/BRCC3 79184 NM_001018055 64762483 GAAGGACCGAGUAGAAAUU L-005798-00 J-005798-15 CXORF53/BRCC3 79184 NM_001018055 64762483 GCAUAUACUGGAACUGAAA
NCI-H2009 (continued)
L-004944-00 J-004944-06 CAMK4 814 NM_001744 27477118 CAUGUGGUCUGUAGGAAUA L-004944-00 J-004944-07 CAMK4 814 NM_001744 27477118 CAAAGAAACGGCUGACUAC L-004944-00 J-004944-08 CAMK4 814 NM_001744 27477118 GGUGCUACAUCCAUUGUGU L-004944-00 J-004944-09 CAMK4 814 NM_001744 27477118 GCAGAUGCCGUUAAACAAA L-019154-00 J-019154-05 CDK5RAP2 55755 NM_001011649 58535452 GCAAAGAAGCUACGAGAUU L-019154-00 J-019154-06 CDK5RAP2 55755 NM_001011649 58535452 CCACUGAACAUAUCUACAA L-019154-00 J-019154-07 CDK5RAP2 55755 NM_001011649 58535452 GCAAGGAUCUGAAUUUGUU L-019154-00 J-019154-08 CDK5RAP2 55755 NM_001011649 58535452 CAGAUACGAUUCAUUAGUU L-008529-00 J-008529-05 CHD1 1105 NM_001270 68299794 CACAAGAGCUGGAGGUCUA L-008529-00 J-008529-06 CHD1 1105 NM_001270 68299794 GAUGAAGAUUGGCAAAUGU L-008529-00 J-008529-07 CHD1 1105 NM_001270 68299794 CGAUCUCAUUUCUGAAUUA L-008529-00 J-008529-08 CHD1 1105 NM_001270 68299794 GUACCGCUCUCCACUCUUA L-017349-00 J-017349-05 CHD6 84181 NM_032221 29244923 GUAGAUAUGUGCAGUCUUA L-017349-00 J-017349-06 CHD6 84181 NM_032221 29244923 GAUAUUGGGUUCAGGAAGA L-017349-00 J-017349-07 CHD6 84181 NM_032221 29244923 GAGUAGCCAUGAUGUGAAA L-017349-00 J-017349-08 CHD6 84181 NM_032221 29244923 CCAACAACGACACCAAUUA L-031153-00 J-031153-05 CHD9 80205 NM_025134 49355789 GAAAGUAUUUGAUGGAGUU L-031153-00 J-031153-06 CHD9 80205 NM_025134 49355789 GAAUGUGGCCCUCAUGAUA L-031153-00 J-031153-07 CHD9 80205 NM_025134 49355789 GUACGUGGACUGAUAUUAA L-031153-00 J-031153-08 CHD9 80205 NM_025134 49355789 GCAAUUGAAUGGCGAUGUG
NCI-H2009 (continued)
L-004613-00 J-004613-09 CIT 11113 NM_007174 32698687 GAAUUUAGUCGGCGUCUUA L-004613-00 J-004613-10 CIT 11113 NM_007174 32698687 CCUCAUACCAGGAUAAAUU L-004613-00 J-004613-11 CIT 11113 NM_007174 32698687 GGAGCAGUCUUCCAAAUUU L-004613-00 J-004613-12 CIT 11113 NM_007174 32698687 GGAGUGAUCUCUACGAAUC L-006153-00 J-006153-06 CLCN5 1184 NM_000084 4557472 GGGCAAGUAUCCUGUUAUA L-006153-00 J-006153-07 CLCN5 1184 NM_000084 4557472 CCGAAGAGAUCUCAUUAUU L-006153-00 J-006153-08 CLCN5 1184 NM_000084 4557472 GAAUGGACCGUCUUCAAUA L-006153-00 J-006153-09 CLCN5 1184 NM_000084 4557472 GAAUGGAACAGCUGGCUUA L-003110-00 J-003110-10 CSK 1445 NM_004383 4758077 GCGAGUGCCUUAUCCAAGA L-003110-00 J-003110-11 CSK 1445 NM_004383 4758077 ACGAGGAGGUGUACUUUGA L-003110-00 J-003110-12 CSK 1445 NM_004383 4758077 UCAAGUGCAUUAAGAACGA L-003110-00 J-003110-13 CSK 1445 NM_004383 4758077 GAAAUUCUCCACUAAGUCU L-020300-00 J-020300-05 CTNNA3 29119 NM_013266 7019570 GCAUACAACUGAUGUGAUC L-020300-00 J-020300-06 CTNNA3 29119 NM_013266 7019570 UAAGCUGGAUGCUGAGAUU L-020300-00 J-020300-07 CTNNA3 29119 NM_013266 7019570 CGGAAGGUGUAAUGAGAAA L-020300-00 J-020300-08 CTNNA3 29119 NM_013266 7019570 GGAGGAAGCAACUUGGAAU L-024917-02 J-024917-11 CXORF23 256643 NM_198279 115511056 GAGGGAAACCACCGAAACA L-024917-02 J-024917-19 CXORF23 256643 NM_198279 115511056 GCUGAACACUACAAGCAAA L-024917-02 J-024917-20 CXORF23 256643 NM_198279 115511056 GCGAUCUAGUAAUGACAAA L-024917-02 J-024917-21 CXORF23 256643 NM_198279 115511056 AGGCUAAGUAUCAGCGUUU
NCI-H2009 (continued)
L-006712-00 J-006712-06 DGKB 1607 NM_145695 22027633 GAGGAUAAGCUUGAGUUUA L-006712-00 J-006712-07 DGKB 1607 NM_145695 22027633 ACGAAGCCAUCGACGAAUA L-006712-00 J-006712-08 DGKB 1607 NM_145695 22027633 CGUCAGGUUUACAGUCUUU L-006712-00 J-006712-09 DGKB 1607 NM_145695 22027633 GCAACAAGUUUCCUCAUUC L-003120-00 J-003120-10 EPHA8 2046 NM_001006943 55770891 GGGACACACCCAAGAUGUA L-003120-00 J-003120-11 EPHA8 2046 NM_001006943 55770891 GCGCGUCUAUGCUGAGAUC L-003120-00 J-003120-12 EPHA8 2046 NM_001006943 55770891 GAAGUGCCCUGCCAUGGUG L-003120-00 J-003120-13 EPHA8 2046 NM_001006943 55770891 GAACUACUCCUUCUGGAUC L-003121-00 J-003121-09 EPHB1 2047 NM_004441 55770893 GAAACGGGCUUAUAGCAAA L-003121-00 J-003121-10 EPHB1 2047 NM_004441 55770893 GCACCUGUCGGACCGGUUA L-003121-00 J-003121-11 EPHB1 2047 NM_004441 55770893 UUACGAGGAUCCCAACGAA L-003121-00 J-003121-12 EPHB1 2047 NM_004441 55770893 CAACGGCAAUGGCAUGAGA L-031861-00 J-031861-05 ERBB2IP 55914 NM_001006600 55770894 GAACAAGUACUUCGACAUA L-031861-00 J-031861-06 ERBB2IP 55914 NM_001006600 55770894 UGACCCAGCUGGAAAGAUU L-031861-00 J-031861-07 ERBB2IP 55914 NM_001006600 55770894 CUAUGUGGCUCUCAGAUAA L-031861-00 J-031861-08 ERBB2IP 55914 NM_001006600 55770894 CGAAGAGCCAAAUAUAAUA L-016075-02 J-016075-18 FLJ39827/FAM123B 139285 NM_152424 124244055 CCAAUAGUGAUGAAGGUUA L-016075-02 J-016075-19 FLJ39827/FAM123B 139285 NM_152424 124244055 GAAAUUAGGAACUGCAGUA L-016075-02 J-016075-20 FLJ39827/FAM123B 139285 NM_152424 124244055 GGAUAAUGGGUUCAGUAAA L-016075-02 J-016075-21 FLJ39827/FAM123B 139285 NM_152424 124244055 UGGGAGUACCCGUGAACAA
NCI-H2009 (continued)
L-012457-00 J-012457-05 FBXL7 23194 NM_012304 21071079 GCAAACAGAUUUCCAUCCG L-012457-00 J-012457-06 FBXL7 23194 NM_012304 21071079 UCAGGAUGCUCCAAAGUGA L-012457-00 J-012457-07 FBXL7 23194 NM_012304 21071079 GUCUCAGGCUGUUACAAUA L-012457-00 J-012457-08 FBXL7 23194 NM_012304 21071079 CAACCCGGCUUUCUUCUGA L-020989-00 J-020989-05 FBXO34 55030 NM_017943 40807492 CAACAGAUAUGAUGUGGAA L-020989-00 J-020989-06 FBXO34 55030 NM_017943 40807492 GGGAGGCCUCUGUCAGUUA L-020989-00 J-020989-07 FBXO34 55030 NM_017943 40807492 AAUAACAGCUUCCGUCGAA L-020989-00 J-020989-08 FBXO34 55030 NM_017943 40807492 UUCAAGAGCUGAUCGUUGU L-008431-01 J-008431-09 FGD2 221472 NM_173558 34222232 UGAAGUACCACCACCGAUU L-008431-01 J-008431-10 FGD2 221472 NM_173558 34222232 GGGCCGAACUGAAAUACGA L-008431-01 J-008431-11 FGD2 221472 NM_173558 34222232 GCAGAGAAUUCCACGUUAC L-008431-01 J-008431-12 FGD2 221472 NM_173558 34222232 CCAUUGACCAAAUCGAGAA L-003132-00 J-003132-17 FGFR2 2263 NM_022976 13186266 GAAUACGGGUCCAUCAAUC L-003132-00 J-003132-18 FGFR2 2263 NM_022976 13186266 AAAGUGUGGUCCCAUCUGA L-003132-00 J-003132-19 FGFR2 2263 NM_022976 13186266 GCACCAUACUGGACCAACA L-003132-00 J-003132-20 FGFR2 2263 NM_022976 13186266 GAGGCUACAAGGUACGAAA L-003133-00 J-003133-09 FGFR3 2261 NM_022965 13112047 CGCCAAGCCUGUCACCGUA L-003133-00 J-003133-10 FGFR3 2261 NM_022965 13112047 GACCGUGUCCUUACCGUGA L-003133-00 J-003133-11 FGFR3 2261 NM_022965 13112047 GAACAAGUUUGGCAGCAUC L-003133-00 J-003133-12 FGFR3 2261 NM_022965 13112047 UGUCGGACCUGGUGUCUGA
NCI-H2009 (continued)
L-024802-02 J-024802-17 KIAA1817/FRMPD3 84443 XM_001714381 169217193 GAACUUAUCAGGAGCGCUA L-024802-02 J-024802-18 KIAA1817/FRMPD3 84443 XM_001714381 169217193 CCGAGGAGGUGUACCGGAA L-024802-02 J-024802-19 KIAA1817/FRMPD3 84443 XM_001714381 169217193 GGUACAGGGAGAACGAACA L-024802-02 J-024802-20 KIAA1817/FRMPD3 84443 XM_001714381 169217193 GCUCCAGUAUCUACGAAUU L-006181-00 J-006181-07 GLRA2 2742 NM_002063 40254460 CCUGAUAUUUGAGUGGUUA L-006181-00 J-006181-08 GLRA2 2742 NM_002063 40254460 AGACGUUACUCGUGAAAGU L-006181-00 J-006181-09 GLRA2 2742 NM_002063 40254460 CAGGACGGCUUUCUGCAAA L-006181-00 J-006181-10 GLRA2 2742 NM_002063 40254460 AGAAAUAGAUGUGCCCUAC L-010633-00 J-010633-07 GOLGA5 9950 NM_005113 30260187 GCAAUUACACUGGCCGAAA L-010633-00 J-010633-08 GOLGA5 9950 NM_005113 30260187 GGACCUAAAUCUACGUAUA L-010633-00 J-010633-09 GOLGA5 9950 NM_005113 30260187 AAACUAAUCUGGCAGGAAU L-010633-00 J-010633-10 GOLGA5 9950 NM_005113 30260187 UCGCAAAGCUGCUAGUUCA L-004515-00 J-004515-06 GUCY2F 2986 NM_001522 4504218 AGGCUUACCUUAUCGCAUU L-004515-00 J-004515-07 GUCY2F 2986 NM_001522 4504218 GCUAAAGUCUCGAAACUGU L-004515-00 J-004515-08 GUCY2F 2986 NM_001522 4504218 GAUGCAGUGUUGACCAUUA L-004515-00 J-004515-09 GUCY2F 2986 NM_001522 4504218 GAUCAAAGGACCCAAUAGA L-009498-01 J-009498-09 HEPH 9843 NM_014799 21166383 CUUACUGGAAAGUGCGAUA L-009498-01 J-009498-10 HEPH 9843 NM_014799 21166383 CAGAAGAGCUCCAGCCGAA L-009498-01 J-009498-11 HEPH 9843 NM_014799 21166383 GAGAAAGCUACGACGCAAU L-009498-01 J-009498-12 HEPH 9843 NM_014799 21166383 CCUGGUACCUGGUUAAUUA
NCI-H2009 (continued)
L-007181-00 J-007181-06 HERC1 8925 NM_003922 62422572 GCACCGACCUUAUUGUGUA L-007181-00 J-007181-07 HERC1 8925 NM_003922 62422572 UAGAUUAGCUUCUGAGUUG L-007181-00 J-007181-08 HERC1 8925 NM_003922 62422572 CCACAGGUCCUAUUACUAA L-007181-00 J-007181-09 HERC1 8925 NM_003922 62422572 GAACAAAGGAACCACUUGA L-004018-00 J-004018-07 HIF1A 3091 NM_181054 31077210 GAACAAAUACAUGGGAUUA L-004018-00 J-004018-08 HIF1A 3091 NM_181054 31077210 AGAAUGAAGUGUACCCUAA L-004018-00 J-004018-09 HIF1A 3091 NM_181054 31077210 GAUGGAAGCACUAGACAAA L-004018-00 J-004018-10 HIF1A 3091 NM_181054 31077210 CAAGUAGCCUCUUUGACAA L-021084-01 J-021084-09 HSPA14 51182 NM_016299 82880657 GGGCUGGUGUGGUUGCAAA L-021084-01 J-021084-10 HSPA14 51182 NM_016299 82880657 CCUGUGUGGCCGUCUAUAA L-021084-01 J-021084-11 HSPA14 51182 NM_016299 82880657 AUGAAGAGAUUGUUGGAUU L-021084-01 J-021084-12 HSPA14 51182 NM_016299 82880657 GUUGUUGCUUACUCAGAAA L-007988-00 J-007988-07 IL3RA 3563 NM_002183 28416950 CAGAUAAGUUUGUCGUCUU L-007988-00 J-007988-08 IL3RA 3563 NM_002183 28416950 UGUUAAAGACGCCGACUAU L-007988-00 J-007988-09 IL3RA 3563 NM_002183 28416950 GAAAUGUGACCGAUAUCGA L-007988-00 J-007988-10 IL3RA 3563 NM_002183 28416950 GAGCGCAGCCUUCGGUAUC L-005332-00 J-005332-05 INSRR 3645 NM_014215 31657139 GGGAGCAGAUCUCGAUAAU L-005332-00 J-005332-06 INSRR 3645 NM_014215 31657139 CGUAAUAGCAGCAGCAUAU L-005332-00 J-005332-07 INSRR 3645 NM_014215 31657139 GAUGGGAACUACACUCUCU L-005332-00 J-005332-08 INSRR 3645 NM_014215 31657139 CAGCGCCUCUGAUAUGUAU
NCI-H2009 (continued)
L-015397-00 J-015397-05 IPO11 51194 NM_016338 39725949 GACGGAAGAUCCUGAAACA L-015397-00 J-015397-06 IPO11 51194 NM_016338 39725949 GAAGUAUAGGUACAGAUAA L-015397-00 J-015397-07 IPO11 51194 NM_016338 39725949 GAUCAUCAAUGGUUAUAUC L-015397-00 J-015397-08 IPO11 51194 NM_016338 39725949 CAGAAUAUGUCACCACUUC L-004760-00 J-004760-12 IRAK1 3654 NM_001025243 68800349 GAUGAGAGGCUGACACCCA L-004760-00 J-004760-13 IRAK1 3654 NM_001025243 68800349 CGAAGAAAGUGAUGAAUUU L-004760-00 J-004760-14 IRAK1 3654 NM_001025243 68800349 GCAAUUCAGUUUCUACAUC L-004760-00 J-004760-15 IRAK1 3654 NM_001025243 68800349 GAGCUGAUGUGUUCACCUG L-010699-00 J-010699-05 ITGA8 8516 NM_003638 49170033 GAAAGUCAGGUCACGAUUA L-010699-00 J-010699-06 ITGA8 8516 NM_003638 49170033 ACAGAAAGAUCAGAGUUAA L-010699-00 J-010699-07 ITGA8 8516 NM_003638 49170033 GCAUAGCAAUUAAGACAUC L-010699-00 J-010699-08 ITGA8 8516 NM_003638 49170033 GGACGCUCUUCCUUGAUAA L-008011-00 J-008011-06 ITGB4 3691 NM_001005619 54607026 GUGGAUGAGUUCCGGAAUA L-008011-00 J-008011-07 ITGB4 3691 NM_001005619 54607026 ACGAGAAGCUUCACACCUA L-008011-00 J-008011-08 ITGB4 3691 NM_001005619 54607026 GUACCCGUAUUGCGACUAU L-008011-00 J-008011-09 ITGB4 3691 NM_001005619 54607026 GUACAGCGAUGACGUUCUA L-006278-00 J-006278-06 KCNV1 27012 NM_014379 28329444 GCUCGCAGGCGUUCCGAUA L-006278-00 J-006278-07 KCNV1 27012 NM_014379 28329444 GGCCUUGCCUAUUGCUAUU L-006278-00 J-006278-08 KCNV1 27012 NM_014379 28329444 GAUGUCAGCUGAGUUAAGC L-006278-00 J-006278-09 KCNV1 27012 NM_014379 28329444 UAACUUGAGAGAUGUCUAU L-020378-01 J-020378-09 JMJD1B/KDM3B 51780 NM_016604 54873608 GAUCAUAUGCAAACGACUA L-020378-01 J-020378-10 JMJD1B/KDM3B 51780 NM_016604 54873608 CUGCACAAGUGUCGUGAAU L-020378-01 J-020378-11 JMJD1B/KDM3B 51780 NM_016604 54873608 CAGAAUAUACCAAACGAGA L-020378-01 J-020378-12 JMJD1B/KDM3B 51780 NM_016604 54873608 GGGGUGAAGUAGACAGUAA
NCI-H2009 (continued)
L-005069-00 J-005069-08 KRAS2 3845 NM_004985 34485723 GGAGGGCUUUCUUUGUGUA L-005069-00 J-005069-09 KRAS2 3845 NM_004985 34485723 UCAAAGACAAAGUGUGUAA L-005069-00 J-005069-10 KRAS2 3845 NM_004985 34485723 GAAGUUAUGGAAUUCCUUU L-005069-00 J-005069-11 KRAS2 3845 NM_004985 34485723 GAGAUAACACGAUGCGUAU L-032755-02 J-032755-21 LOC139604/MAGEB16 139604 NM_001099921 153791872 GCAGAUAUGUUGAAGAUUA L-032755-02 J-032755-22 LOC139604/MAGEB16 139604 NM_001099921 153791872 GGCCAAAGUUCAUGGGUCU L-032755-02 J-032755-23 LOC139604/MAGEB16 139604 NM_001099921 153791872 CUGAUGAGGCUUCCAGCAA L-032755-02 J-032755-24 LOC139604/MAGEB16 139604 NM_001099921 153791872 CGGGAGUAUAUUCUGGGAA L-011554-00 J-011554-06 MBD4 8930 NM_003925 4505120 GAAGAUUUGAUGUGUACUU L-011554-00 J-011554-07 MBD4 8930 NM_003925 4505120 GGAACAGAAUGCCGUAAGU L-011554-00 J-011554-08 MBD4 8930 NM_003925 4505120 GAAGAUACCAUCCCACGAA L-011554-00 J-011554-09 MBD4 8930 NM_003925 4505120 UAACUUUACUUCCACUCAU L-004828-00 J-004828-06 MLL2 8085 NM_003482 4505196 GCAAAUCGCUAGCAUCAUU L-004828-00 J-004828-07 MLL2 8085 NM_003482 4505196 GAUAUUAGCUACCACACAU L-004828-00 J-004828-08 MLL2 8085 NM_003482 4505196 GAAAGGGCACUGAGGGAUA L-004828-00 J-004828-09 MLL2 8085 NM_003482 4505196 GCAUGAAGCCGCAGCAAUU L-015299-01 J-015299-09 MOBKL2A 126308 NM_130807 40018625 CCGAAGAGAAGCACGCUGU L-015299-01 J-015299-10 MOBKL2A 126308 NM_130807 40018625 UGUUACUAGCAACCGGAAA L-015299-01 J-015299-11 MOBKL2A 126308 NM_130807 40018625 GAGCAUAAGUUCCGGAAGC L-015299-01 J-015299-12 MOBKL2A 126308 NM_130807 40018625 UGGACUGGAUCGAGGCGCA L-007668-00 J-007668-05 MYH9 4627 NM_002473 51317362 GUAUCAAUGUGACCGAUUU L-007668-00 J-007668-06 MYH9 4627 NM_002473 51317362 CAAAGGAGCCCUGGCGUUA L-007668-00 J-007668-07 MYH9 4627 NM_002473 51317362 GGAGGAACGCCGAGCAGUA L-007668-00 J-007668-08 MYH9 4627 NM_002473 51317362 CGAAGCGGGUGAAAGCAAA
NCI-H2009 (continued)
L-020159-00 J-020159-05 NCOA2 10499 NM_006540 76253684 GGACAAGGGUUGAAUAUGA L-020159-00 J-020159-06 NCOA2 10499 NM_006540 76253684 GGAGAUUGAUAGAGCCUUA L-020159-00 J-020159-07 NCOA2 10499 NM_006540 76253684 CAACACAGGCACCUAUUAA L-020159-00 J-020159-08 NCOA2 10499 NM_006540 76253684 CAGAAUCGCCAGCCACUUA L-019113-00 J-019113-05 NALP10/NLRP10 338322 NM_176821 78191799 CGCAGGAUCUGAAGCAUUU L-019113-00 J-019113-06 NALP10/NLRP10 338322 NM_176821 78191799 GCUCCUAUUUCACGGAUGA L-019113-00 J-019113-07 NALP10/NLRP10 338322 NM_176821 78191799 GAGAGGCAAAGUUGUCUUA L-019113-00 J-019113-08 NALP10/NLRP10 338322 NM_176821 78191799 GGAGGGCAAAGAUAAUAUA L-017367-00 J-017367-05 CIAS1/NLRP3 114548 NM_183395 34878689 GGAUCAAACUACUCUGUGA L-017367-00 J-017367-06 CIAS1/NLRP3 114548 NM_183395 34878689 UGCAAGAUCUCUCAGCAAA L-017367-00 J-017367-07 CIAS1/NLRP3 114548 NM_183395 34878689 GAAGUGGGGUUCAGAUAAU L-017367-00 J-017367-08 CIAS1/NLRP3 114548 NM_183395 34878689 GCAAGACCAAGACGUGUGA L-012235-00 J-012235-05 NOTCH2 4853 NM_024408 24041034 GGAGAUGACUGCAGUGAGA L-012235-00 J-012235-06 NOTCH2 4853 NM_024408 24041034 GAACGAUGGUCGCUGCAUU L-012235-00 J-012235-07 NOTCH2 4853 NM_024408 24041034 GUGAGUGUCUGAAGGGUUA L-012235-00 J-012235-08 NOTCH2 4853 NM_024408 24041034 GAAGGUACCCUGGUUAUUG L-005356-00 J-005356-06 NRBP/NRBP1 29959 NM_013392 50726964 GGAAUGAGGUACAGUUCUC L-005356-00 J-005356-07 NRBP/NRBP1 29959 NM_013392 50726964 GCAAUGGAGAGUCCUCAUA L-005356-00 J-005356-08 NRBP/NRBP1 29959 NM_013392 50726964 UGGAGCAUCUUAACAUUGU L-005356-00 J-005356-09 NRBP/NRBP1 29959 NM_013392 50726964 CAGAGGGAGUUCAUUCAAA
NCI-H2009 (continued)
L-009345-00 J-009345-05 NTHL1 4913 NM_002528 38455392 ACACGCAUGUGCACAGAAU L-009345-00 J-009345-06 NTHL1 4913 NM_002528 38455392 CGUGAAGCGUCCGCGGAAA L-009345-00 J-009345-07 NTHL1 4913 NM_002528 38455392 GGAGCAAGGUGAAAUACAU L-009345-00 J-009345-08 NTHL1 4913 NM_002528 38455392 CGAGAUCAAUGGACUCUUG L-003160-00 J-003160-09 NTRK2 4915 NM_001018064 65506744 GGGAACAUCUCUCGGUCUA L-003160-00 J-003160-10 NTRK2 4915 NM_001018064 65506744 GCGCUUCAGUGGUUCUAUA L-003160-00 J-003160-11 NTRK2 4915 NM_001018064 65506744 GAAUGAAACAAGCCACACA L-003160-00 J-003160-12 NTRK2 4915 NM_001018064 65506744 GUGAUCCGGUUCCUAAUAU L-029990-01 J-029990-09 NUP160 23279 NM_015231 54859721 GCCUUAACUUCCACGGAUA L-029990-01 J-029990-10 NUP160 23279 NM_015231 54859721 UGACAAGGUUUUACGACUA L-029990-01 J-029990-11 NUP160 23279 NM_015231 54859721 GGACUACUUAUACGGGAAA L-029990-01 J-029990-12 NUP160 23279 NM_015231 54859721 GAUCAUAAACUACGAAUGU L-020767-01 J-020767-09 NUP93 9688 NM_014669 41281436 CGGCAGAUGUCAAGGCGUC L-020767-01 J-020767-10 NUP93 9688 NM_014669 41281436 GCUCAAGGAAUAAGCGCAA L-020767-01 J-020767-11 NUP93 9688 NM_014669 41281436 AGAGUGAAGUGGCGGACAA L-020767-01 J-020767-12 NUP93 9688 NM_014669 41281436 GAUAAGAGCAUUUCCGACA L-020639-01 J-020639-09 ODZ1 10178 NM_014253 7657410 CUAUAGAUCUUUACCGAUA L-020639-01 J-020639-10 ODZ1 10178 NM_014253 7657410 AGAAUUAACUUACGAGCUU L-020639-01 J-020639-11 ODZ1 10178 NM_014253 7657410 GGAGCAAGUAUUCGUGUUA L-020639-01 J-020639-12 ODZ1 10178 NM_014253 7657410 GAAUGGAGAAUACGAGAAA
NCI-H2009 (continued)
L-005021-00 J-005021-07 PDK3 5165 NM_005391 4885544 GUACUGAUGCUGUCAUUUA L-005021-00 J-005021-08 PDK3 5165 NM_005391 4885544 GGGCUACCCUGCUGUUAAA L-005021-00 J-005021-09 PDK3 5165 NM_005391 4885544 GUUGGUAUAUGCAGAGUUU L-005021-00 J-005021-10 PDK3 5165 NM_005391 4885544 CGGGAGAGAUAAUGCAUGU L-006772-00 J-006772-06 PIK3C2B 5287 NM_002646 15451925 GUUCGACACUUACCACAAU L-006772-00 J-006772-07 PIK3C2B 5287 NM_002646 15451925 GCUACCAGCUAUGAAGAUU L-006772-00 J-006772-08 PIK3C2B 5287 NM_002646 15451925 CAACUGUUCCUCCACUGUA L-006772-00 J-006772-09 PIK3C2B 5287 NM_002646 15451925 GAGCUAAACGGUUACAUCU L-005250-00 J-005250-09 PIK3C3 5289 NM_002647 34761063 CACCAAAGCUCAUCGACAA L-005250-00 J-005250-10 PIK3C3 5289 NM_002647 34761063 AUAGAUAGCUCCCAAAUUA L-005250-00 J-005250-11 PIK3C3 5289 NM_002647 34761063 GAACAACGGUUUCGCUCUU L-005250-00 J-005250-12 PIK3C3 5289 NM_002647 34761063 GAGAUGUACUUGAACGUAA L-008770-00 J-008770-05 PIP5KL1 138429 NM_173492 27735084 CCGAGAGGUAUGACAUCAA L-008770-00 J-008770-06 PIP5KL1 138429 NM_173492 27735084 GCACAGAGCCCGGAAGAGU L-008770-00 J-008770-07 PIP5KL1 138429 NM_173492 27735084 GCUGAAGGACCUCAACUUU L-008770-00 J-008770-08 PIP5KL1 138429 NM_173492 27735084 GAUUACAGCCUCCUGAUAG L-010280-00 J-010280-05 PLCB1 23236 NM_015192 33356545 GAGAAUAGCAGUUUAUGAA L-010280-00 J-010280-06 PLCB1 23236 NM_015192 33356545 GCACUUAAUUUCCAGACAA L-010280-00 J-010280-07 PLCB1 23236 NM_015192 33356545 GAACACACUACCAAGUAUA L-010280-00 J-010280-08 PLCB1 23236 NM_015192 33356545 CAUCGAAGCUUUAUCAAAC
NCI-H2009 (continued)
L-003559-00 J-003559-05 PLCG1 5335 NM_182811 33598945 GCAGCAAGAUCUACUACUC L-003559-00 J-003559-06 PLCG1 5335 NM_182811 33598945 GAUGGGAUGCCAGUUAUUU L-003559-00 J-003559-07 PLCG1 5335 NM_182811 33598945 GAGACAACCGCCUCUAGUU L-003559-00 J-003559-08 PLCG1 5335 NM_182811 33598945 GAAUGGAAUUUCGCCUGAA L-005111-00 J-005111-05 PPARGC1A 10891 NM_013261 29570796 GAGAAUUCAUGGAGCAAUA L-005111-00 J-005111-06 PPARGC1A 10891 NM_013261 29570796 GAAGAGCGCCGUGUGAUUU L-005111-00 J-005111-07 PPARGC1A 10891 NM_013261 29570796 ACACUCAGCUAAGUUAUAA L-005111-00 J-005111-08 PPARGC1A 10891 NM_013261 29570796 GCAGGUAACAUGUUCCCUA L-012612-00 J-012612-05 PPFIA3 8541 NM_003660 41872688 AGGCGUAACUCCCGACUCA L-012612-00 J-012612-06 PPFIA3 8541 NM_003660 41872688 GGUGGACGCUCGAAUGUUA L-012612-00 J-012612-07 PPFIA3 8541 NM_003660 41872688 CGAGGAACGUCAUGGGAAU L-012612-00 J-012612-08 PPFIA3 8541 NM_003660 41872688 GAUGAACGAUGACCACAAU L-010063-01 J-010063-09 PREX1 57580 NM_020820 34452731 AAGAUGGGACAGCGGAUUA L-010063-01 J-010063-10 PREX1 57580 NM_020820 34452731 CCACGGACAUCAUGCGGAA L-010063-01 J-010063-11 PREX1 57580 NM_020820 34452731 ACGUCAUGAUUGCGGAGAA L-010063-01 J-010063-12 PREX1 57580 NM_020820 34452731 CCUUUAAGCAGCUGGACGA L-014602-00 J-014602-05 DEPDC2/PREX2 80243 NM_025170 47578116 GAAGCGGACUCCACGGAAA L-014602-00 J-014602-06 DEPDC2/PREX2 80243 NM_025170 47578116 GAACACCUAUGUCAGAGAA L-014602-00 J-014602-07 DEPDC2/PREX2 80243 NM_025170 47578116 CAAGAUACCUGGGUCAUGA L-014602-00 J-014602-08 DEPDC2/PREX2 80243 NM_025170 47578116 GAAGCGAAUUUGUGUCAUG
NCI-H2009 (continued)
L-004443-00 J-004443-06 AMID/PRG-3 84883 NM_032797 31563505 GAGUGGAGAUGGCAGCAGA L-004443-00 J-004443-07 AMID/PRG-3 84883 NM_032797 31563505 GGAGACAGGGUUCGCCAAA L-004443-00 J-004443-08 AMID/PRG-3 84883 NM_032797 31563505 GGGCAAGUUUAAUGAGGUU L-004443-00 J-004443-09 AMID/PRG-3 84883 NM_032797 31563505 CAAUGAGUAUCGAGAGUAC L-005030-00 J-005030-06 PRKDC 5591 NM_006904 31340617 GGAAGAAGCUCAUUUGAUU L-005030-00 J-005030-07 PRKDC 5591 NM_006904 31340617 GAGCAUCACUUGCCUUUAA L-005030-00 J-005030-08 PRKDC 5591 NM_006904 31340617 GCAGGACCGUGCAAGGUUA L-005030-00 J-005030-09 PRKDC 5591 NM_006904 31340617 AGAUAGAGCUGCUAAAUGU L-005366-00 J-005366-05 PSKH2 85481 NM_033126 14916454 GCAGAUAACUCAUAGGAAU L-005366-00 J-005366-06 PSKH2 85481 NM_033126 14916454 GCUCUUUGAUCGACUCAUU L-005366-00 J-005366-07 PSKH2 85481 NM_033126 14916454 GAUCACAUAUGCUUUACUU L-005366-00 J-005366-08 PSKH2 85481 NM_033126 14916454 GGUUAGCCAUCGUUACAUU L-004994-00 J-004994-07 PTPRB 5787 NM_002837 18491009 GAACAAUUCCGGUCGUAAU L-004994-00 J-004994-08 PTPRB 5787 NM_002837 18491009 GAGAAUACAUUGUCACUCA L-004994-00 J-004994-09 PTPRB 5787 NM_002837 18491009 UCACAGAGAUCCAGUCUAU L-004994-00 J-004994-10 PTPRB 5787 NM_002837 18491009 GCAACCGAGUAUCGAUUUA L-006326-00 J-006326-05 PTPRM 5797 NM_002845 18860903 GGACUUGCCUGGCGACUUU L-006326-00 J-006326-06 PTPRM 5797 NM_002845 18860903 GCAAUUAUAUCGAUGGUUA L-006326-00 J-006326-07 PTPRM 5797 NM_002845 18860903 GAACGUCCUCGAAGAACUA L-006326-00 J-006326-08 PTPRM 5797 NM_002845 18860903 GAGUGAGGCUGCAGACAAU
NCI-H2009 (continued)
L-008072-00 J-008072-06 PTPRT 11122 NM_007050 48762923 GGAGGCUGGUGUUCGAUUA L-008072-00 J-008072-07 PTPRT 11122 NM_007050 48762923 GGUCUGGUGUGUCCAACUA L-008072-00 J-008072-08 PTPRT 11122 NM_007050 48762923 CCAACUACAUUGACGGAUA L-008072-00 J-008072-09 PTPRT 11122 NM_007050 48762923 GAAGAUGGCUGGCGUGAUC L-009685-00 J-009685-05 PTPRZ1 5803 NM_002851 4506328 GAGAUUGGCUGGUCCUAUA L-009685-00 J-009685-06 PTPRZ1 5803 NM_002851 4506328 GAACAUAUCCCAAGGGUAU L-009685-00 J-009685-07 PTPRZ1 5803 NM_002851 4506328 GAGCUGUACUGUUGACUUA L-009685-00 J-009685-08 PTPRZ1 5803 NM_002851 4506328 GUUCUUAGAUCUCCACAUA L-006832-00 J-006832-06 RAD21 5885 NM_006265 5453993 GCUCAGCCUUUGUGGAAUA L-006832-00 J-006832-07 RAD21 5885 NM_006265 5453993 GGGAGUAGUUCGAAUCUAU L-006832-00 J-006832-08 RAD21 5885 NM_006265 5453993 GACCAAGGUUCCAUAUUAU L-006832-00 J-006832-09 RAD21 5885 NM_006265 5453993 GCAUUGGAGCCUAUUGAUA L-005232-00 J-005232-06 RAD50 10111 NM_133482 19924130 ACAAGGAUCUGGAUAUUUA L-005232-00 J-005232-07 RAD50 10111 NM_133482 19924130 UAACCUCACUGUUGGGAUA L-005232-00 J-005232-08 RAD50 10111 NM_133482 19924130 GAAUUGAAUCGUAAGCUUA L-005232-00 J-005232-09 RAD50 10111 NM_133482 19924130 GACACAAGGUCAGAAAGUA L-017467-00 J-017467-05 RAD51L3 5892 NM_133629 19924124 CCACAUAACUCGAGACAGG L-017467-00 J-017467-06 RAD51L3 5892 NM_133629 19924124 GAUCAGACAUGACCUGUGC L-017467-00 J-017467-07 RAD51L3 5892 NM_133629 19924124 GGCCAAAUCUUCCCGACAG L-017467-00 J-017467-08 RAD51L3 5892 NM_133629 19924124 AGAAAUGUGGCUUGUCUUA
NCI-H2009 (continued)
L-008593-00 J-008593-05 RAPGEF6 51735 NM_016340 54633318 GAAUGGUCAUCUCCGGUUA L-008593-00 J-008593-06 RAPGEF6 51735 NM_016340 54633318 GGUCAAAGAUGAUGCAUAA L-008593-00 J-008593-07 RAPGEF6 51735 NM_016340 54633318 GAGAAGGGAUUUGGUAUUU L-008593-00 J-008593-08 RAPGEF6 51735 NM_016340 54633318 GGAGAGCGCCAAACCAUUA L-003296-00 J-003296-10 RB1 5925 NM_000321 4506434 CGAAAUCAGUGUCCAUAAA L-003296-00 J-003296-11 RB1 5925 NM_000321 4506434 GAGUUGACCUAGAUGAGAU L-003296-00 J-003296-12 RB1 5925 NM_000321 4506434 AAACUACGCUUUGAUAUUG L-003296-00 J-003296-13 RB1 5925 NM_000321 4506434 GAAUCUGCUUGUCCUCUUA L-012008-00 J-012008-05 RBBP5 5929 NM_005057 53759147 UAACACGGCAGAUCGAAUA L-012008-00 J-012008-06 RBBP5 5929 NM_005057 53759147 UAUAGAACUUCAAGGAGUA L-012008-00 J-012008-07 RBBP5 5929 NM_005057 53759147 GCAAUACCACAGCCAUUAA L-012008-00 J-012008-08 RBBP5 5929 NM_005057 53759147 GAUGGAACUUUGGAUUGUA L-003298-00 J-003298-10 RBL1 5933 NM_183404 34577076 GCGAUUAUGUGCCCAAAUA L-003298-00 J-003298-11 RBL1 5933 NM_183404 34577076 UCAAAUGAGUUACGAAGGA L-003298-00 J-003298-12 RBL1 5933 NM_183404 34577076 AGGCUAAUGUGGAGUAUAA L-003298-00 J-003298-13 RBL1 5933 NM_183404 34577076 GCUAAUAGUCACGUAUAUA L-006466-00 J-006466-10 REST 5978 NM_005612 15890082 GGUGAAACUUUAAAUGGUA L-006466-00 J-006466-11 REST 5978 NM_005612 15890082 GAAUCUCACUGGUAUAAAU L-006466-00 J-006466-12 REST 5978 NM_005612 15890082 CAUCCUACUUGUCCUAAUA L-006466-00 J-006466-13 REST 5978 NM_005612 15890082 AGACAUAUGCGUACUCAUU
NCI-H2009 (continued)
L-003170-00 J-003170-09 RET 5979 NM_020630 50593520 CAGGAGGGCUCGCCGAUUU L-003170-00 J-003170-10 RET 5979 NM_020630 50593520 AGAGACGGCUGGAGUGUGA L-003170-00 J-003170-11 RET 5979 NM_020630 50593520 GGGCGACCGUACAUGACUA L-003170-00 J-003170-12 RET 5979 NM_020630 50593520 GUCCCGAGAUGUUUAUGAA L-020480-00 J-020480-06 RHOBTB3 22836 NM_014899 41281538 ACAGAUGGCCGUCGAAUAU L-020480-00 J-020480-07 RHOBTB3 22836 NM_014899 41281538 CCGGAAAUGUCGUUGCUUA L-020480-00 J-020480-08 RHOBTB3 22836 NM_014899 41281538 GAAGAGUCCCACUGACAUU L-020480-00 J-020480-09 RHOBTB3 22836 NM_014899 41281538 CCAUGAACCUUGAUAUAGU L-004663-00 J-004663-06 RPS6KA2 6196 NM_001006932 55743135 CAAGCGAUGUGUGCAUAAA L-004663-00 J-004663-07 RPS6KA2 6196 NM_001006932 55743135 UAAUGGAGCUGAUGCGUGG L-004663-00 J-004663-08 RPS6KA2 6196 NM_001006932 55743135 GGAACACGCUGUACCGGAA L-004663-00 J-004663-09 RPS6KA2 6196 NM_001006932 55743135 GCAAAUGGGCCAGACGAUA L-005318-00 J-005318-05 FLJ10074/SCYL2 55681 NM_017988 51870926 GAUUCAACAUGCCCGUUAA L-005318-00 J-005318-06 FLJ10074/SCYL2 55681 NM_017988 51870926 GCAAUGGGCUAGCUUGGAA L-005318-00 J-005318-07 FLJ10074/SCYL2 55681 NM_017988 51870926 GCUUUGAUACCAAGAAUUA L-005318-00 J-005318-08 FLJ10074/SCYL2 55681 NM_017988 51870926 GAGCAUCACUUACACUUGA L-007580-02 J-007580-18 SLC4A10 57282 NM_022058 155722997 AAAGAAAGGUUGUGGGUAC L-007580-02 J-007580-19 SLC4A10 57282 NM_022058 155722997 CCUAAUGACAGAUGAGGUA L-007580-02 J-007580-20 SLC4A10 57282 NM_022058 155722997 CUAUAAAGCUAAAGAUCGU L-007580-02 J-007580-21 SLC4A10 57282 NM_022058 155722997 GGAUAGAGGUGGAACUCGU
NCI-H2009 (continued)
L-008312-00 J-008312-05 SMARCC2 6601 NM_139067 21237807 UCACUAAACUGCCGAUCAA L-008312-00 J-008312-06 SMARCC2 6601 NM_139067 21237807 GAGAAGCACUGGAGUAUCA L-008312-00 J-008312-07 SMARCC2 6601 NM_139067 21237807 GCUACUAUCCUGACAGUUA L-008312-00 J-008312-08 SMARCC2 6601 NM_139067 21237807 UAAGAAAGGUCCCUCAACA L-009459-00 J-009459-06 SMARCD3 6604 NM_001003802 51477703 GAAGAAGACGGCGUGCUAU L-009459-00 J-009459-07 SMARCD3 6604 NM_001003802 51477703 UGACAGAUGUAGCCGGCAA L-009459-00 J-009459-08 SMARCD3 6604 NM_001003802 51477703 GGCAGUAUGUGAAGACCAA L-009459-00 J-009459-09 SMARCD3 6604 NM_001003802 51477703 GAUCAAACCAUCAUGCGGA L-005385-00 J-005385-10 TAF1L 138474 NM_153809 24429571 CCAAGCAACUUCUACGUAA L-005385-00 J-005385-11 TAF1L 138474 NM_153809 24429571 GAUCAGAGCUAAGGUGUCA L-005385-00 J-005385-12 TAF1L 138474 NM_153809 24429571 GAUGAAGGGUGGAUUAGGA L-005385-00 J-005385-13 TAF1L 138474 NM_153809 24429571 CUAGGAAUGUAAUGGCUUA L-003932-00 J-003932-05 TIAM1 7074 NM_003253 4507500 GCAGGCUACUGUCGGAAUU L-003932-00 J-003932-06 TIAM1 7074 NM_003253 4507500 GUAUAAAGAUGGUUCCAAA L-003932-00 J-003932-07 TIAM1 7074 NM_003253 4507500 GAACCGAAGCUGUAAAGAA L-003932-00 J-003932-08 TIAM1 7074 NM_003253 4507500 CAAAUUAGCCAUAGCAACA L-011111-00 J-011111-06 TIMP3 7078 NM_000362 75905820 GCUGACAGGUCGCGUCUAU L-011111-00 J-011111-07 TIMP3 7078 NM_000362 75905820 GGGCUGAACUAUCGGUAUC L-011111-00 J-011111-08 TIMP3 7078 NM_000362 75905820 CCGACAUGCUCUCCAAUUU L-011111-00 J-011111-09 TIMP3 7078 NM_000362 75905820 GCUACUACCUGCCUUGCUU
NCI-H2009 (continued)
L-003102-01 J-003102-18 TNK2 10188 NM_001010938 58331190 GGUGUUCAGUGGAAAGCGA L-003102-01 J-003102-19 TNK2 10188 NM_001010938 58331190 CCUAUGACCCUGUGAGCGA L-003102-01 J-003102-20 TNK2 10188 NM_001010938 58331190 CGGACAGGAUUGACGAACU L-003102-01 J-003102-21 TNK2 10188 NM_001010938 58331190 CGAAGUUGCUGCUCCGGCA L-004741-00 J-004741-05 TNKS2 80351 NM_025235 21361945 UAGCAUAACUCAAUUCGUA L-004741-00 J-004741-06 TNKS2 80351 NM_025235 21361945 GGAAAGACGUAGUUGAAUA L-004741-00 J-004741-07 TNKS2 80351 NM_025235 21361945 GAGGGUAUCUCAUUAGGUA L-004741-00 J-004741-08 TNKS2 80351 NM_025235 21361945 AGACAGAUCUUGUUACAUU L-004240-00 J-004240-07 TOP2B 7155 NM_001068 19913407 GAAGUUGUCUGUUGAGAGA L-004240-00 J-004240-08 TOP2B 7155 NM_001068 19913407 CGAAAGACCUAAAUACACA L-004240-00 J-004240-09 TOP2B 7155 NM_001068 19913407 GAUCAUAUGGGAUGUCUGA L-004240-00 J-004240-10 TOP2B 7155 NM_001068 19913407 GGUGUAUGAUGAAGAUGUA L-003329-00 J-003329-14 TP53 7157 NM_000546 8400737 GAAAUUUGCGUGUGGAGUA L-003329-00 J-003329-15 TP53 7157 NM_000546 8400737 GUGCAGCUGUGGGUUGAUU L-003329-00 J-003329-16 TP53 7157 NM_000546 8400737 GCAGUCAGAUCCUAGCGUC L-003329-00 J-003329-17 TP53 7157 NM_000546 8400737 GGAGAAUAUUUCACCCUUC L-009863-00 J-009863-05 TPO 7173 NM_175722 28558989 CCAGAUGACUUGUGAGAAC L-009863-00 J-009863-06 TPO 7173 NM_175722 28558989 GGUUAUGGCCUGCACAGAA L-009863-00 J-009863-07 TPO 7173 NM_175722 28558989 GUACGUGGGUCCCUAUGAA L-009863-00 J-009863-08 TPO 7173 NM_175722 28558989 GCACGGGUAUGAGCUCCAA
NCI-H2009 (continued)
L-006587-00 J-006587-05 TRIM26 7726 NM_003449 60279912 GCAUCGCCCUGGAUUAUGA L-006587-00 J-006587-06 TRIM26 7726 NM_003449 60279912 CUAAACAGGUAUCCACGGA L-006587-00 J-006587-07 TRIM26 7726 NM_003449 60279912 UGAGAGACUUGGAAUAUAA L-006587-00 J-006587-08 TRIM26 7726 NM_003449 60279912 CAACGAGGCCUGAGGGAAU L-005392-00 J-005392-08 TRIM33 51592 NM_033020 74027250 GGACAAACCACAUUAGUAA L-005392-00 J-005392-09 TRIM33 51592 NM_033020 74027250 GGAAAGCAGUUGCAUUGUA L-005392-00 J-005392-10 TRIM33 51592 NM_033020 74027250 GAACAUAGGUAUCAGUUUU L-005392-00 J-005392-11 TRIM33 51592 NM_033020 74027250 CCAGCAAGUUGGUGUAAUA L-009644-00 J-009644-05 TRPS1 7227 NM_014112 7657658 GAAUGCAAAUGGCGGAUAU L-009644-00 J-009644-06 TRPS1 7227 NM_014112 7657658 CAACUCAUCCACCGAAUUA L-009644-00 J-009644-07 TRPS1 7227 NM_014112 7657658 GCUGGAAGCUCGCGAGUCA L-009644-00 J-009644-08 TRPS1 7227 NM_014112 7657658 CCACAGAUCUGAUUAAGCA L-014119-01 J-014119-09 ZNF537/TSHZ3 57616 NM_020856 24308230 ACACUUAGGCUUCCGGCUA L-014119-01 J-014119-10 ZNF537/TSHZ3 57616 NM_020856 24308230 CAGACCACCUCGACCGCUA L-014119-01 J-014119-11 ZNF537/TSHZ3 57616 NM_020856 24308230 ACAGAGAGCCACACGUCAA L-014119-01 J-014119-12 ZNF537/TSHZ3 57616 NM_020856 24308230 GGCAUUACCGCGACGACAA L-012556-00 J-012556-05 TYRP1 7306 NM_000550 4507756 GCGCACAACUCACCCUUUA L-012556-00 J-012556-06 TYRP1 7306 NM_000550 4507756 CUUGGAAGUUGGUUUAUUU L-012556-00 J-012556-07 TYRP1 7306 NM_000550 4507756 GGACAUAAUAGACAAUACA L-012556-00 J-012556-08 TYRP1 7306 NM_000550 4507756 UAAUCAGUCUGUGGUCUAA
NCI-H2009 (continued)
L-007189-00 J-007189-06 EDD1/UBR5 51366 NM_015902 41352716 GCACUUAUAUACUGGAUUA L-007189-00 J-007189-07 EDD1/UBR5 51366 NM_015902 41352716 GAUUGUAGGUUACUUAGAA L-007189-00 J-007189-08 EDD1/UBR5 51366 NM_015902 41352716 GAUCAAUCCUAACUGAAUU L-007189-00 J-007189-09 EDD1/UBR5 51366 NM_015902 41352716 GGUCGAAGAUGUGCUACUA L-006086-00 J-006086-06 USP38 84640 NM_032557 31559774 GAUCAGAAGUAUCAUGUGA L-006086-00 J-006086-07 USP38 84640 NM_032557 31559774 UCACAUACGUUGUUUGAAC L-006086-00 J-006086-08 USP38 84640 NM_032557 31559774 ACUAAUGGUUUAAGUGGUA L-006086-00 J-006086-09 USP38 84640 NM_032557 31559774 GUAACUUGCUGCAGAACAU L-018913-01 J-018913-09 WDR44 54521 NM_019045 45238857 GUAUAAGGGUUACGUCAAU L-018913-01 J-018913-10 WDR44 54521 NM_019045 45238857 CUUCAGAAAGUACGGUUAA L-018913-01 J-018913-11 WDR44 54521 NM_019045 45238857 AUACAAUACUGAAGGACGU L-018913-01 J-018913-12 WDR44 54521 NM_019045 45238857 ACUGUUAGCCAUAGAUCAA L-006571-01 J-006571-14 WHSC1 7468 NM_007331 6594682 GGUCCAAAGUGUCGGGUUA L-006571-01 J-006571-15 WHSC1 7468 NM_007331 6594682 UGUCAGUGGAGGAGCGGAA L-006571-01 J-006571-16 WHSC1 7468 NM_007331 6594682 GGAGCAGGGCCUUGUCGAA L-006571-01 J-006571-17 WHSC1 7468 NM_007331 6594682 CCGGGUGUUUAAUGGAGAA L-012386-00 J-012386-05 WIF1 11197 NM_007191 18379354 GUACGAAGCCAGCCUCAUA L-012386-00 J-012386-06 WIF1 11197 NM_007191 18379354 CAGCACACGCCUUCACUUA L-012386-00 J-012386-07 WIF1 11197 NM_007191 18379354 GUGCCGAAAUGGAGGCUUU L-012386-00 J-012386-08 WIF1 11197 NM_007191 18379354 GGCAUCAGUUGUUCAAGUU
NCI-H2009 (continued)
L-004098-00 J-004098-14 BIRC4/XIAP 331 NM_001167 32528298 GUAGAUAGAUGGCAAUAUG L-004098-00 J-004098-15 BIRC4/XIAP 331 NM_001167 32528298 GAACUGGGCAGGUUGUAGA L-004098-00 J-004098-16 BIRC4/XIAP 331 NM_001167 32528298 GAAAGAGAUUAGUACUGAA L-004098-00 J-004098-17 BIRC4/XIAP 331 NM_001167 32528298 GGACUCUACUACACAGGUA L-011939-00 J-011939-05 ZFYVE9 9372 NM_007323 6552336 GCUGUAGCCUGAAAUGUAA L-011939-00 J-011939-06 ZFYVE9 9372 NM_007323 6552336 GAUGAAUGAUAGCCAAGUA L-011939-00 J-011939-07 ZFYVE9 9372 NM_007323 6552336 GAAGCUAGAGUGUGUGUAA L-011939-00 J-011939-08 ZFYVE9 9372 NM_007323 6552336 AGAUGCAGGUCUAGAUUUA Origene SR302910A MAP3K9/MLK1 4293 NM_033141 126632195 GGAAGAGUCUUAUCAACAGCCGCTC Origene SR302910B MAP3K9/MLK1 4293 NM_033141 126632195 ACCUUUGAAUAGAGUGUUAUCUGGG Origene SR302910C MAP3K9/MLK1 4293 NM_033141 126632195 CCCAUUCAGUUGUUAGAAAUUGATT Ambion S8806 MAP3K9/MLK1 4293 NM_033141 126632195 GACCAUCUUUCACGAAUAU
ON-TARGETplus SMARTpool siRNA library NCI-H2087
Duplex Pool Catalog Gene GI Catalog Gene Symbol GENE ID Sequence Number Accession Number Number L-005301-00 J-005301-06 AATK 9625 XM_927215 89041906 UAAAGAGGCUUGAGACCUG L-005301-00 J-005301-07 AATK 9625 XM_927215 89041906 CCAAAUGGCUCCACAGCGG L-005301-00 J-005301-08 AATK 9625 XM_927215 89041906 GACGACGACUUCCCGCUGA L-005301-00 J-005301-09 AATK 9625 XM_927215 89041906 GAACGCUGGUGCAAUGUUU L-022416-00 J-022416-05 ADAMTSL3 57188 NM_207517 46447826 GUUCAGAUGUGGAAAGUUC L-022416-00 J-022416-06 ADAMTSL3 57188 NM_207517 46447826 CAAUCAAAGUCACACGUUU L-022416-00 J-022416-07 ADAMTSL3 57188 NM_207517 46447826 CCUGGUAACUGGUCACAUU L-022416-00 J-022416-08 ADAMTSL3 57188 NM_207517 46447826 GAUGAUGACCACAUUAGUA L-008115-00 J-008115-05 ADH7 131 NM_000673 71743839 CAGCGUGGUUGUAGGAGUU L-008115-00 J-008115-06 ADH7 131 NM_000673 71743839 GCUGGUGCAUCUAGGAUCA L-008115-00 J-008115-07 ADH7 131 NM_000673 71743839 GCAAACCAGUCCACCACUU L-008115-00 J-008115-08 ADH7 131 NM_000673 71743839 GAAGUUAUUGGGCAUCUUG L-003201-00 J-003201-11 ATM 472 NM_138292 73486662 GCAAAGCCCUAGUAACAUA L-003201-00 J-003201-12 ATM 472 NM_138292 73486662 GGUGUGAUCUUCAGUAUAU L-003201-00 J-003201-13 ATM 472 NM_138292 73486662 GAGAGGAGACAGCUUGUUA L-003201-00 J-003201-14 ATM 472 NM_138292 73486662 GAUGGGAGGCCUAGGAUUU L-005434-00 J-005434-05 BAI3 577 NM_001704 4502358 GAUAAACGCUCCAUAUUGU L-005434-00 J-005434-06 BAI3 577 NM_001704 4502358 GGACAGAUUUCGGGAUAUA L-005434-00 J-005434-07 BAI3 577 NM_001704 4502358 GAUGGGAGAUCAUACAAUU L-005434-00 J-005434-08 BAI3 577 NM_001704 4502358 CAUCAUAAGCCUUGUAAUA
NCI-H2087 (continued)
N/A TROWA-000019 BRAF 673 NM_004333 187608632 AAAGAAUUGGAUCUGGAUCAUUU L-003217-00 J-003217-10 CCNG2 901 NM_004354 4757935 CAUGAUGUGAUCCGGAUUA L-003217-00 J-003217-11 CCNG2 901 NM_004354 4757935 CUAAAUGCCUAGCCGAGUA L-003217-00 J-003217-12 CCNG2 901 NM_004354 4757935 CGGAGAAUGAUAACACUUU L-003217-00 J-003217-13 CCNG2 901 NM_004354 4757935 GAAGUUGGUUUGGAUCGUU L-003220-00 J-003220-10 CCNT1 904 NM_001240 17978465 UAGUAUACAUGCAUCGAUU L-003220-00 J-003220-11 CCNT1 904 NM_001240 17978465 GAACAAACGUCCUGGUGAU L-003220-00 J-003220-12 CCNT1 904 NM_001240 17978465 CAAGCAACUUAGCACAUAA L-003220-00 J-003220-13 CCNT1 904 NM_001240 17978465 GCCCAUAGAGGGUUCAGAA L-014368-00 J-014368-05 CHD1L 9557 NM_004284 42734376 GGGAAGACCUGCCAGACUA L-014368-00 J-014368-06 CHD1L 9557 NM_004284 42734376 GGUGGGAAUCCAACAAUUA L-014368-00 J-014368-07 CHD1L 9557 NM_004284 42734376 GGACAUGCCACGAAAGGUU L-014368-00 J-014368-08 CHD1L 9557 NM_004284 42734376 AGAGACACUUGGCCAUUAA L-007869-02 J-007869-17 CSMD3 114788 NM_052900 38045885 CGAAGUGGAUCCUCAGAAA L-007869-02 J-007869-18 CSMD3 114788 NM_052900 38045885 CGGAUAAGAUAUAUAGCUU L-007869-02 J-007869-19 CSMD3 114788 NM_052900 38045885 GGAUUAUAAAUUAGGGUCA L-007869-02 J-007869-20 CSMD3 114788 NM_052900 38045885 AAUAAUAGCAGAAGAACGA L-006712-00 J-006712-06 DGKB 1607 NM_145695 22027633 GAGGAUAAGCUUGAGUUUA L-006712-00 J-006712-07 DGKB 1607 NM_145695 22027633 ACGAAGCCAUCGACGAAUA L-006712-00 J-006712-08 DGKB 1607 NM_145695 22027633 CGUCAGGUUUACAGUCUUU L-006712-00 J-006712-09 DGKB 1607 NM_145695 22027633 GCAACAAGUUUCCUCAUUC
NCI-H2087 (continued)
L-006713-00 J-006713-10 DGKD 8527 NM_003648 25777595 GAUCAUAGAACACACAGAA L-006713-00 J-006713-11 DGKD 8527 NM_003648 25777595 CAGCGAGGAUUCCGAGGUA L-006713-00 J-006713-12 DGKD 8527 NM_003648 25777595 GGUGGAGCGUCAUGGCAUA L-006713-00 J-006713-13 DGKD 8527 NM_003648 25777595 AGAUUGGAUUGCAGCAUUA L-011253-00 J-011253-05 DOCK1 1793 NM_001380 50345995 GUACCGAGGUUACACGUUA L-011253-00 J-011253-06 DOCK1 1793 NM_001380 50345995 GAAAGUCGAUGGUGGUGAA L-011253-00 J-011253-07 DOCK1 1793 NM_001380 50345995 UAAAUGAGCAGCUGUACAA L-011253-00 J-011253-08 DOCK1 1793 NM_001380 50345995 GGCCCAAGCCUGACUAUUU L-005315-00 J-005315-08 EPHA5 2044 NM_182472 32967318 CGAAAUAGCAGGCCCAAGU L-005315-00 J-005315-09 EPHA5 2044 NM_182472 32967318 UAUGGUGUCUUCAGUCGAA L-005315-00 J-005315-10 EPHA5 2044 NM_182472 32967318 UGAGUCAGAUGAUCAGAAU L-005315-00 J-005315-11 EPHA5 2044 NM_182472 32967318 CAUCAUCCAUUUAGAAGGU L-031861-00 J-031861-05 ERBB2IP 55914 NM_001006600 55770894 GAACAAGUACUUCGACAUA L-031861-00 J-031861-06 ERBB2IP 55914 NM_001006600 55770894 UGACCCAGCUGGAAAGAUU L-031861-00 J-031861-07 ERBB2IP 55914 NM_001006600 55770894 CUAUGUGGCUCUCAGAUAA L-031861-00 J-031861-08 ERBB2IP 55914 NM_001006600 55770894 CGAAGAGCCAAAUAUAAUA L-010942-00 J-010942-05 ELKS/ERC1 23085 NM_178038 38045893 GCGGACAAUUGAACGCUUA L-010942-00 J-010942-06 ELKS/ERC1 23085 NM_178038 38045893 UGAAAGAACGGGUCAAAUC L-010942-00 J-010942-07 ELKS/ERC1 23085 NM_178038 38045893 CAAUAUAGCUCUCUUGGAG L-010942-00 J-010942-08 ELKS/ERC1 23085 NM_178038 38045893 GCACAAAUGUUAGAGGAGG
NCI-H2087 (continued)
L-017621-00 J-017621-05 FBLN5 10516 NM_006329 19743802 GGACGAGUGUGCAACAGAU L-017621-00 J-017621-06 FBLN5 10516 NM_006329 19743802 GCAGACGUGCUACAAUUUA L-017621-00 J-017621-07 FBLN5 10516 NM_006329 19743802 GAUACCAGAUGGAUGAAAG L-017621-00 J-017621-08 FBLN5 10516 NM_006329 19743802 GUGCAAACCUGCGUCAACA L-020989-00 J-020989-05 FBXO34 55030 NM_017943 40807492 CAACAGAUAUGAUGUGGAA L-020989-00 J-020989-06 FBXO34 55030 NM_017943 40807492 GGGAGGCCUCUGUCAGUUA L-020989-00 J-020989-07 FBXO34 55030 NM_017943 40807492 AAUAACAGCUUCCGUCGAA L-020989-00 J-020989-08 FBXO34 55030 NM_017943 40807492 UUCAAGAGCUGAUCGUUGU L-023753-00 J-023753-05 FBXO46 23403 XM_371179 51474742 UGACGGGCGUGGUAGAUGA L-023753-00 J-023753-06 FBXO46 23403 XM_371179 51474742 GGACGUUCCUACUGGAUGU L-023753-00 J-023753-07 FBXO46 23403 XM_371179 51474742 CUAGAGAUCCGCUUCAAGA L-023753-00 J-023753-08 FBXO46 23403 XM_371179 51474742 GAUAACAACUGGGUGCUGC L-010359-00 J-010359-06 FOXP2 93986 NM_014491 17017962 GGGCUAGACCUCACUACUA L-010359-00 J-010359-07 FOXP2 93986 NM_014491 17017962 AAGUAAUCCUGGACUGAUA L-010359-00 J-010359-08 FOXP2 93986 NM_014491 17017962 GUCACCAUGUCGAAGAAUA L-010359-00 J-010359-09 FOXP2 93986 NM_014491 17017962 GACAGGCAGUUAACACUUA L-015377-00 J-015377-06 FZR1 51343 NM_016263 33620741 CCACAGGAUUAACGAGAAU L-015377-00 J-015377-07 FZR1 51343 NM_016263 33620741 GGAACACGCUGACAGGACA L-015377-00 J-015377-08 FZR1 51343 NM_016263 33620741 GCAACGAUGUGUCUCCCUA L-015377-00 J-015377-09 FZR1 51343 NM_016263 33620741 GAAGAAGGGUCUGUUCACG
NCI-H2087 (continued)
L-009024-00 J-009024-05 GATA2 2624 NM_032638 31982886 GCAAGGCUCGUUCCUGUUC L-009024-00 J-009024-06 GATA2 2624 NM_032638 31982886 GAAGGGAUCCAGACUCGGA L-009024-00 J-009024-07 GATA2 2624 NM_032638 31982886 GCACAAUGUUAACAGGCCA L-009024-00 J-009024-08 GATA2 2624 NM_032638 31982886 GGGCAGAACCGACCACUCA L-005515-00 J-005515-05 GLP1R 2740 NM_002062 19923203 GCUGGCGGCUUGAGCACUU L-005515-00 J-005515-06 GLP1R 2740 NM_002062 19923203 GAAAUGGCGAGAAUACCGA L-005515-00 J-005515-07 GLP1R 2740 NM_002062 19923203 GCCCUGAAGUGGAUGUAUA L-005515-00 J-005515-08 GLP1R 2740 NM_002062 19923203 GGAACUCCAACAUGAACUA L-005578-00 J-005578-05 GPR50 9248 NM_004224 4758467 CACCAUCGUUGUAGACCUA L-005578-00 J-005578-06 GPR50 9248 NM_004224 4758467 CAAUCGCUAUCAACCGUUA L-005578-00 J-005578-07 GPR50 9248 NM_004224 4758467 CAACUGGCUUUAUCUUGCA L-005578-00 J-005578-08 GPR50 9248 NM_004224 4758467 GGGUGACUCUGUCCAUUUC L-009330-00 J-009330-07 HDAC7A 51564 NM_016596 13259523 GACAAGAGCAAGCGAAGUG L-009330-00 J-009330-08 HDAC7A 51564 NM_016596 13259523 GCAGAUACCCUCGGCUGAA L-009330-00 J-009330-09 HDAC7A 51564 NM_016596 13259523 GGUGAGGGCUUCAAUGUCA L-009330-00 J-009330-10 HDAC7A 51564 NM_016596 13259523 UGGCUGCUCUUCUGGGUAA L-007186-00 J-007186-05 HECW1 23072 NM_015052 51702517 CUAAAUGACUGGCGGAAUA L-007186-00 J-007186-06 HECW1 23072 NM_015052 51702517 GAUGAGGUCUUGUCCGAAA L-007186-00 J-007186-07 HECW1 23072 NM_015052 51702517 GAUGCCAGCUCGUACUUUG L-007186-00 J-007186-08 HECW1 23072 NM_015052 51702517 CAGCUGCAAUUCCGAUUUG
NCI-H2087 (continued)
L-009498-01 J-009498-09 HEPH 9843 NM_014799 21166383 CUUACUGGAAAGUGCGAUA L-009498-01 J-009498-10 HEPH 9843 NM_014799 21166383 CAGAAGAGCUCCAGCCGAA L-009498-01 J-009498-11 HEPH 9843 NM_014799 21166383 GAGAAAGCUACGACGCAAU L-009498-01 J-009498-12 HEPH 9843 NM_014799 21166383 CCUGGUACCUGGUUAAUUA L-005175-00 J-005175-07 HERC6 55008 NM_001013000 61563737 GAAGAGAGGUCCACAACUU L-005175-00 J-005175-08 HERC6 55008 NM_001013000 61563737 AAGAAUUGAUGGCCUAGUU L-005175-00 J-005175-09 HERC6 55008 NM_001013000 61563737 GAAUCUAGGUGUGGUUUAU L-005175-00 J-005175-10 HERC6 55008 NM_001013000 61563737 CAGAACCAAUUCAGGCAUU L-006945-00 J-006945-05 ZNFN1A3/IKZF3 22806 NM_183231 38045964 GAGCGUGCCUUCUGAGAGA L-006945-00 J-006945-06 ZNFN1A3/IKZF3 22806 NM_183231 38045964 GGAGAUGGUUCCAGUUAUC L-006945-00 J-006945-07 ZNFN1A3/IKZF3 22806 NM_183231 38045964 AAUCACAUCUAUCAGCAAA L-006945-00 J-006945-08 ZNFN1A3/IKZF3 22806 NM_183231 38045964 AGACAUAGGAGAUGAUUCA L-007999-00 J-007999-09 ITGA10 8515 NM_003637 38569397 GUUCAGACCUUCCUACGAA L-007999-00 J-007999-10 ITGA10 8515 NM_003637 38569397 GCACUAGGAGAUCGGAUUU L-007999-00 J-007999-11 ITGA10 8515 NM_003637 38569397 UGUCUCAAGUCAUCACUAA L-007999-00 J-007999-12 ITGA10 8515 NM_003637 38569397 GAUACAGUGUCUUACAACA L-004566-00 J-004566-06 ITGA2 3673 NM_002203 6006008 GAACGGGACUUUCGCAUCA L-004566-00 J-004566-07 ITGA2 3673 NM_002203 6006008 GAAACGCCCUUGAUACUAA L-004566-00 J-004566-08 ITGA2 3673 NM_002203 6006008 GUUCAGACCUACUAAGCAA L-004566-00 J-004566-09 ITGA2 3673 NM_002203 6006008 AAACAAGGCUGAUAAUUUG
NCI-H2087 (continued)
L-007214-00 J-007214-05 ITGA6 3655 NM_000210 4557674 GGAUCGAGUUUGAUAACGA L-007214-00 J-007214-06 ITGA6 3655 NM_000210 4557674 GGAUAUGCCUCCAGGUUAA L-007214-00 J-007214-07 ITGA6 3655 NM_000210 4557674 GAAAGGGAUUGUUCGUGUA L-007214-00 J-007214-08 ITGA6 3655 NM_000210 4557674 ACAGAUAGAUGAUAACAGA L-010699-00 J-010699-05 ITGA8 8516 NM_003638 49170033 GAAAGUCAGGUCACGAUUA L-010699-00 J-010699-06 ITGA8 8516 NM_003638 49170033 ACAGAAAGAUCAGAGUUAA L-010699-00 J-010699-07 ITGA8 8516 NM_003638 49170033 GCAUAGCAAUUAAGACAUC L-010699-00 J-010699-08 ITGA8 8516 NM_003638 49170033 GGACGCUCUUCCUUGAUAA L-006208-00 J-006208-05 ITPR2 3709 NM_002223 4504792 CGAAAUGGCCGCUCUAUUA L-006208-00 J-006208-06 ITPR2 3709 NM_002223 4504792 GCAACAAGAGCAAUAAGUA L-006208-00 J-006208-07 ITPR2 3709 NM_002223 4504792 GCAAUGGGUUGGAGACUAU L-006208-00 J-006208-08 ITPR2 3709 NM_002223 4504792 GGAAUGAAAGGGCAAUUAA L-006829-00 J-006829-05 KNTC1 9735 NM_014708 41327744 GUAAAUAACUUGCGAGAGU L-006829-00 J-006829-06 KNTC1 9735 NM_014708 41327744 GAUAAAGCAUGGCAGAAUU L-006829-00 J-006829-07 KNTC1 9735 NM_014708 41327744 CUCAAGAGAUGCUGAAUUA L-006829-00 J-006829-08 KNTC1 9735 NM_014708 41327744 GGAGCUAGCCCUAAGAUUU L-005322-00 J-005322-06 KSR2 283455 NM_173598 34222392 GAGCAAAUCCCACGAGUUC L-005322-00 J-005322-07 KSR2 283455 NM_173598 34222392 GAAGCCACCUCGCUAUUCA L-005322-00 J-005322-08 KSR2 283455 NM_173598 34222392 GAGGAGAUCCAGCAAGGUU L-005322-00 J-005322-09 KSR2 283455 NM_173598 34222392 GGAGAAAUACGGAGCCAAC
NCI-H2087 (continued)
L-020010-00 J-020010-05 LHFP 10186 NM_005780 5031864 CUACACUUCUGGCCAGUUU L-020010-00 J-020010-06 LHFP 10186 NM_005780 5031864 UGGAGGAUCUGCACCAUAG L-020010-00 J-020010-07 LHFP 10186 NM_005780 5031864 GGAGGAAUUCAGUUUCUUG L-020010-00 J-020010-08 LHFP 10186 NM_005780 5031864 GCACUACCCAUACUGAGAU L-031636-02 J-031636-13 LOC389904 389904 XM_001716796 169216754 CGGGAAUGAACUCGAAUUU L-031636-02 J-031636-14 LOC389904 389904 XM_001716796 169216754 AGAAUGAAAUUGCCAAGAU L-031636-02 J-031636-15 LOC389904 389904 XM_001716796 169216754 ACUUAGUCUUGGCAGCAUA L-031636-02 J-031636-16 LOC389904 389904 XM_001716796 169216754 GCUCGGGUCUAGACGGCUU L-015997-01 J-015997-09 FLJ32786/LRGUK 136332 NM_144648 21389482 GUUACAGUUUCGCGCAGAA L-015997-01 J-015997-10 FLJ32786/LRGUK 136332 NM_144648 21389482 CGUAGAAGGUAUCGCAAGA L-015997-01 J-015997-11 FLJ32786/LRGUK 136332 NM_144648 21389482 ACAAUAAGAUCACGACAAU L-015997-01 J-015997-12 FLJ32786/LRGUK 136332 NM_144648 21389482 GAGGGAUAUUUGCGGAGAA L-031609-02 J-031609-17 LOC392433/MAGEB6B 392433 XM_373340 41151618 CAAGAUGAGGAAAGUUUAA L-031609-02 J-031609-18 LOC392433/MAGEB6B 392433 XM_373340 41151618 GCAGGUAUUUCAUGCUCAA L-031609-02 J-031609-19 LOC392433/MAGEB6B 392433 XM_373340 41151618 CGUGGGAAACGCCGAGAGA L-031609-02 J-031609-20 LOC392433/MAGEB6B 392433 XM_373340 41151618 UUACAACCACAGACGGAGA L-003904-00 J-003904-06 MCF2 4168 NM_005369 19923309 GAACUGGGCAAGAUGAUAA L-003904-00 J-003904-07 MCF2 4168 NM_005369 19923309 UAACAGAACGGGAUAAGUU L-003904-00 J-003904-08 MCF2 4168 NM_005369 19923309 GAAGAAGGCACUCGAUGCA L-003904-00 J-003904-09 MCF2 4168 NM_005369 19923309 GAGCUCAGUUAGUGAUUUG
NCI-H2087 (continued)
L-011081-00 J-011081-05 MEFV 4210 NM_000243 4557742 GCCCGCAAAUCCAGAAAUU L-011081-00 J-011081-06 MEFV 4210 NM_000243 4557742 GCUACUGGGUGGUGAUAAU L-011081-00 J-011081-07 MEFV 4210 NM_000243 4557742 GCAUAUGACACCCGCGUAU L-011081-00 J-011081-08 MEFV 4210 NM_000243 4557742 GCAGGCCCUUCGAAGUGUA L-031965-00 J-031965-05 RKHD1/MEX3D 399664 NM_203304 42558261 GUAUUGAGUGGUCAGGUUA L-031965-00 J-031965-06 RKHD1/MEX3D 399664 NM_203304 42558261 GAGCCGGUCUUCAUCGUGA L-031965-00 J-031965-07 RKHD1/MEX3D 399664 NM_203304 42558261 GCCGAACACUUCUCCAUCA L-031965-00 J-031965-08 RKHD1/MEX3D 399664 NM_203304 42558261 GCGACAAGGAGCCGGUGUU L-011737-00 J-011737-05 MYH11 4629 NM_002474 13124878 GGACGUAGAGUUAUUGAAA L-011737-00 J-011737-06 MYH11 4629 NM_002474 13124878 GGAAUUAAUGUGACAGAUU L-011737-00 J-011737-07 MYH11 4629 NM_002474 13124878 GAAGGUCACGGUUGGGAAA L-011737-00 J-011737-08 MYH11 4629 NM_002474 13124878 UCACAGGGAUGCUUAACGA L-018862-00 J-018862-05 NCOA7 135112 NM_181782 42476174 GGAAUAGACAUUACCCUUA L-018862-00 J-018862-06 NCOA7 135112 NM_181782 42476174 GAUCAGGCGUACAGAACUA L-018862-00 J-018862-07 NCOA7 135112 NM_181782 42476174 GAAUAUUACCUGACUAAGA L-018862-00 J-018862-08 NCOA7 135112 NM_181782 42476174 GAAUUCAGGUACCCAUUGA L-018073-00 J-018073-05 NALP13/NLRP13 126204 NM_176810 28827788 CCACGUAUAUAUCCGUAAU L-018073-00 J-018073-06 NALP13/NLRP13 126204 NM_176810 28827788 GAAGGGCUGUGGUCUAUGA L-018073-00 J-018073-07 NALP13/NLRP13 126204 NM_176810 28827788 GAUGUUGGGUCGUAUCUUU L-018073-00 J-018073-08 NALP13/NLRP13 126204 NM_176810 28827788 CUACGUUGGUCACAAAUGA
NCI-H2087 (continued)
L-015351-01 J-015351-09 NALP4/NLRP4 147945 NM_134444 54792746 GCUCUGACGCAUACGGAUU L-015351-01 J-015351-10 NALP4/NLRP4 147945 NM_134444 54792746 CUGAAAUGCUUCUGCGUAA L-015351-01 J-015351-11 NALP4/NLRP4 147945 NM_134444 54792746 ACAACAAGAAGCUGACGUA L-015351-01 J-015351-12 NALP4/NLRP4 147945 NM_134444 54792746 CCUAUCAAGCUCACGCAAA L-013446-00 J-013446-05 CAPON/NOS1AP 9722 NM_014697 31317300 GCAAUAUCUUCAGGUGUAA L-013446-00 J-013446-06 CAPON/NOS1AP 9722 NM_014697 31317300 GGACGUGGGAUGAGAGCAA L-013446-00 J-013446-07 CAPON/NOS1AP 9722 NM_014697 31317300 CAUUAUGGUUUCAGUGGAU L-013446-00 J-013446-08 CAPON/NOS1AP 9722 NM_014697 31317300 GGUAUGAGUUUAAAGCCAA L-011883-00 J-011883-05 NOTCH4 4855 NM_004557 55770875 GCACGGACGGUGUCAGUAA L-011883-00 J-011883-06 NOTCH4 4855 NM_004557 55770875 GCAGGAGGGUCCACGUUGU L-011883-00 J-011883-07 NOTCH4 4855 NM_004557 55770875 GGUGAGACGUGCCAGUUUC L-011883-00 J-011883-08 NOTCH4 4855 NM_004557 55770875 GCCCAACCCUGCGAUAAUG L-003919-00 J-003919-05 NRAS 4893 NM_002524 6006027 GAGCAGAUUAAGCGAGUAA L-003919-00 J-003919-06 NRAS 4893 NM_002524 6006027 GAAAUACGCCAGUACCGAA L-003919-00 J-003919-07 NRAS 4893 NM_002524 6006027 GUGGUGAUGUAACAAGAUA L-003919-00 J-003919-08 NRAS 4893 NM_002524 6006027 GCACUGACAAUCCAGCUAA L-003161-00 J-003161-09 NTRK3 4916 NM_002530 59889559 UGUAGUUUCUGGCGGAUUU L-003161-00 J-003161-10 NTRK3 4916 NM_002530 59889559 ACGGACAUCUCAAGGAAUA L-003161-00 J-003161-11 NTRK3 4916 NM_002530 59889559 GGUCGACGGUCCAAAUUUG L-003161-00 J-003161-12 NTRK3 4916 NM_002530 59889559 GCAAGACUGAGAUCAAUUG
NCI-H2087 (continued)
L-005283-00 J-005283-05 NUP153 9972 NM_005124 24430145 CAAUUCGUCUCAAGCAUUA L-005283-00 J-005283-06 NUP153 9972 NM_005124 24430145 GAUAGGAGUGGGAUAGAUA L-005283-00 J-005283-07 NUP153 9972 NM_005124 24430145 CAAGAUAGCAAACUAGCAA L-005283-00 J-005283-08 NUP153 9972 NM_005124 24430145 GUUCCACGCUGGCCAGAAA L-003288-00 J-003288-09 ORC6L 23594 NM_014321 32454755 AGACCUAGCUGUACAGUUU L-003288-00 J-003288-10 ORC6L 23594 NM_014321 32454755 GACAGCAGGUCGACAGAGA L-003288-00 J-003288-11 ORC6L 23594 NM_014321 32454755 GUAGAGGAGAUGCCACAUA L-003288-00 J-003288-12 ORC6L 23594 NM_014321 32454755 GAUCUUGACUUAUCCAGGC L-003973-00 J-003973-13 PAK7/PAK5 57144 NM_177990 29826328 GACAAGCGAUGGCCGGAUA L-003973-00 J-003973-14 PAK7/PAK5 57144 NM_177990 29826328 GGGAAUACUUGGCCAACUU L-003973-00 J-003973-15 PAK7/PAK5 57144 NM_177990 29826328 CAAAGCAAAUCGGGCUAUU L-003973-00 J-003973-16 PAK7/PAK5 57144 NM_177990 29826328 CAAACCAAGUGAAUACAGU L-011978-01 J-011978-09 PLS3 5358 NM_005032 28416938 GAAAGAACCUUCCGUAACU L-011978-01 J-011978-10 PLS3 5358 NM_005032 28416938 CUGAAAAUUUGCCGAAGUA L-011978-01 J-011978-11 PLS3 5358 NM_005032 28416938 GGGAAUGAAGAGAGUGUAA L-011978-01 J-011978-12 PLS3 5358 NM_005032 28416938 CGGAUUCAUUUGUGACUAU L-011358-00 J-011358-05 PPP1R10 5514 NM_002714 25777670 ACAAUUGGCUGACGUAUUC L-011358-00 J-011358-06 PPP1R10 5514 NM_002714 25777670 GCAGACCCGUUCACCAGAA L-011358-00 J-011358-07 PPP1R10 5514 NM_002714 25777670 GAACGAGUAAAUGUGAAUA L-011358-00 J-011358-08 PPP1R10 5514 NM_002714 25777670 GAAACAACCAGACUAUUCG
NCI-H2087 (continued)
L-008072-00 J-008072-06 PTPRT 11122 NM_007050 48762923 GGAGGCUGGUGUUCGAUUA L-008072-00 J-008072-07 PTPRT 11122 NM_007050 48762923 GGUCUGGUGUGUCCAACUA L-008072-00 J-008072-08 PTPRT 11122 NM_007050 48762923 CCAACUACAUUGACGGAUA L-008072-00 J-008072-09 PTPRT 11122 NM_007050 48762923 GAAGAUGGCUGGCGUGAUC L-003601-00 J-003601-13 RAF1/CRAF 5894 NM_002880 52486392 ACAGAGAGAUUCAAGCUAU L-003601-00 J-003601-14 RAF1/CRAF 5894 NM_002880 52486392 AUUCAAAGAUGCCGUGUUU L-003601-00 J-003601-15 RAF1/CRAF 5894 NM_002880 52486392 CAAAGAACAUCAUCCAUAG L-003601-00 J-003601-16 RAF1/CRAF 5894 NM_002880 52486392 GUAAAUGGCACGGAGAUGU L-007021-00 J-007021-05 RNF130 55819 NM_018434 38176162 GUAGAAUGGUUUUGAAGAA L-007021-00 J-007021-06 RNF130 55819 NM_018434 38176162 UAACGUAGCAUUCGAUAUG L-007021-00 J-007021-07 RNF130 55819 NM_018434 38176162 CAAAUGCACGCGACAGGAA L-007021-00 J-007021-08 RNF130 55819 NM_018434 38176162 GUGCCGAAUUUGCCAUGUA L-032052-01 J-032052-09 SERPINA13 388007 NM_207378 46409377 GGACCAGAGAACUCGAUUU L-032052-01 J-032052-10 SERPINA13 388007 NM_207378 46409377 GGAGAAGGACCUCGGCAGU L-032052-01 J-032052-11 SERPINA13 388007 NM_207378 46409377 GGGUAAGGAUAGUAAUGGU L-032052-01 J-032052-12 SERPINA13 388007 NM_207378 46409377 GCAGGGAGCUUCUAUUACA L-012596-00 J-012596-05 SERPINB4 6318 NM_002974 28076868 UGUAGUAGUAGUCGAAUUA L-012596-00 J-012596-06 SERPINB4 6318 NM_002974 28076868 CAACACUGCACAACAAAUU L-012596-00 J-012596-07 SERPINB4 6318 NM_002974 28076868 GUUCAUCACCAGUUUCAAA L-012596-00 J-012596-08 SERPINB4 6318 NM_002974 28076868 CGACACUGGUUCUUGUGAA
NCI-H2087 (continued)
L-004673-00 J-004673-09 SGK2 10110 NM_170693 25168260 GAAAGAGCCUUAUGAUCGA L-004673-00 J-004673-10 SGK2 10110 NM_170693 25168260 GGGAUGACCUGUACCACAA L-004673-00 J-004673-11 SGK2 10110 NM_170693 25168260 GAGAUUAAGAACCAUGUAU L-004673-00 J-004673-12 SGK2 10110 NM_170693 25168260 GUUCUACGCUGCUGAGGUG L-018775-00 J-018775-05 MGC26963/SGMS2 166929 NM_152621 37059795 CAACGGAUUCUACGAUUGA L-018775-00 J-018775-06 MGC26963/SGMS2 166929 NM_152621 37059795 ACACUACACUAUCGAUGUG L-018775-00 J-018775-07 MGC26963/SGMS2 166929 NM_152621 37059795 GGGAUUAUAUUAGUUGGAU L-018775-00 J-018775-08 MGC26963/SGMS2 166929 NM_152621 37059795 UCUCUUCACAGACUAAUUU L-003850-00 J-003850-07 SLK 9748 NM_014720 41281452 GGUAGAGAUUGACAUAUUA L-003850-00 J-003850-08 SLK 9748 NM_014720 41281452 GAAAAGAGCUCAUGAAACG L-003850-00 J-003850-09 SLK 9748 NM_014720 41281452 GCUCGAAGAACGACACUUA L-003850-00 J-003850-10 SLK 9748 NM_014720 41281452 GGAACAUAGCCAAGAAUUA L-013625-00 J-013625-09 SHARP/SPEN 23013 NM_015001 14790189 GGACAAGACAUUACGAUCA L-013625-00 J-013625-10 SHARP/SPEN 23013 NM_015001 14790189 GGAUGGAUCAUGUCGAUUU L-013625-00 J-013625-11 SHARP/SPEN 23013 NM_015001 14790189 UAACCAAGAUCGUACAUAU L-013625-00 J-013625-12 SHARP/SPEN 23013 NM_015001 14790189 GUCAGGAGCUUGCCAGUAU L-003177-00 J-003177-10 TEC 7006 NM_003215 4507428 GAAAUUGUCUAGUAAGUGA L-003177-00 J-003177-11 TEC 7006 NM_003215 4507428 GUACAAAGUCGCAAUCAAA L-003177-00 J-003177-12 TEC 7006 NM_003215 4507428 GUAAUUACGUAACGGGAAA L-003177-00 J-003177-13 TEC 7006 NM_003215 4507428 GAAGGUGGUUUUAUGGUAA
NCI-H2087 (continued)
L-017151-00 J-017151-05 NCOA6IP/TGS1 96764 NM_024831 19923660 GCGGGAGGAUUGUAAGAUA L-017151-00 J-017151-06 NCOA6IP/TGS1 96764 NM_024831 19923660 UAUCGAUCCUGUUAAGAUU L-017151-00 J-017151-07 NCOA6IP/TGS1 96764 NM_024831 19923660 GCACGACGCUUCCACAAGU L-017151-00 J-017151-08 NCOA6IP/TGS1 96764 NM_024831 19923660 UAGGAUAACUGCACAUAAG L-003329-00 J-003329-14 TP53 7157 NM_000546 8400737 GAAAUUUGCGUGUGGAGUA L-003329-00 J-003329-15 TP53 7157 NM_000546 8400737 GUGCAGCUGUGGGUUGAUU L-003329-00 J-003329-16 TP53 7157 NM_000546 8400737 GCAGUCAGAUCCUAGCGUC L-003329-00 J-003329-17 TP53 7157 NM_000546 8400737 GGAGAAUAUUUCACCCUUC L-010548-00 J-010548-06 TPR 7175 NM_003292 4507658 GAAGAAGUGCGUAAGAAUA L-010548-00 J-010548-07 TPR 7175 NM_003292 4507658 UCAGUUGACUCCAGGAAUA L-010548-00 J-010548-08 TPR 7175 NM_003292 4507658 UCAAGGAGGUUUAGGAAUG L-010548-00 J-010548-09 TPR 7175 NM_003292 4507658 GGCAUACACUUACUAGAAA L-005049-00 J-005049-05 ULK1 8408 NM_003565 4507830 CAGCAUCACUGCCGAGAGG L-005049-00 J-005049-06 ULK1 8408 NM_003565 4507830 CCACGCAGGUGCAGAACUA L-005049-00 J-005049-07 ULK1 8408 NM_003565 4507830 GCACAGAGACCGUGGGCAA L-005049-00 J-005049-08 ULK1 8408 NM_003565 4507830 UCACUGACCUGCUCCUUAA L-011939-00 J-011939-05 ZFYVE9 9372 NM_007323 6552336 GCUGUAGCCUGAAAUGUAA L-011939-00 J-011939-06 ZFYVE9 9372 NM_007323 6552336 GAUGAAUGAUAGCCAAGUA L-011939-00 J-011939-07 ZFYVE9 9372 NM_007323 6552336 GAAGCUAGAGUGUGUGUAA L-011939-00 J-011939-08 ZFYVE9 9372 NM_007323 6552336 AGAUGCAGGUCUAGAUUUA L-024656-02 J-024656-19 ZNF630 57232 NM_001037735 170016084 CCACCUAAGUAUAAUGCUU L-024656-02 J-024656-20 ZNF630 57232 NM_001037735 170016084 UCAGAAUCCAUAUAGGAAA L-024656-02 J-024656-21 ZNF630 57232 NM_001037735 170016084 CUGCUUAGGCCCUCAGAAU L-024656-02 J-024656-22 ZNF630 57232 NM_001037735 170016084 GGAUCUACCCAGACAGAGU
ON-TARGETplus SMARTpool siRNA library NCI-H2122
Duplex Pool Catalog Gene GI Catalog Gene Symbol GENE ID Sequence Number Accession Number Number L-009304-00 J-009304-05 ARHGAP6 395 NM_013422 7382477 CAUCAUCGCUGUUGUGCAA L-009304-00 J-009304-06 ARHGAP6 395 NM_013422 7382477 CGGAGAAGCUUUACAGAGU L-009304-00 J-009304-07 ARHGAP6 395 NM_013422 7382477 GGAAUUUGACCGUGGGAUU L-009304-00 J-009304-08 ARHGAP6 395 NM_013422 7382477 GUACACAGCUUUCAUCAAC L-003877-00 J-003877-08 CDH1 999 NM_004360 14589887 GGCCUGAAGUGACUCGUAA L-003877-00 J-003877-09 CDH1 999 NM_004360 14589887 GAGAACGCAUUGCCACAUA L-003877-00 J-003877-10 CDH1 999 NM_004360 14589887 GGGACAACGUUUAUUACUA L-003877-00 J-003877-11 CDH1 999 NM_004360 14589887 GACAAUGGUUCUCCAGUUG L-011007-00 J-011007-08 CDKN2A 1029 NM_058195 47132605 GAUCAUCAGUCACCGAAGG L-011007-00 J-011007-09 CDKN2A 1029 NM_058195 47132605 AAACACCGCUUCUGCCUUU L-011007-00 J-011007-10 CDKN2A 1029 NM_058195 47132605 UAACGUAGAUAUAUGCCUU L-011007-00 J-011007-11 CDKN2A 1029 NM_058195 47132605 CAGAACCAAAGCUCAAAUA L-003477-00 J-003477-06 CREBBP 1387 NM_004380 4758055 GCACAGCCGUUUACCAUGA L-003477-00 J-003477-07 CREBBP 1387 NM_004380 4758055 UCACCAACGUGCCAAAUAU L-003477-00 J-003477-08 CREBBP 1387 NM_004380 4758055 GGGAUGAAGUCACGGUUUG L-003477-00 J-003477-09 CREBBP 1387 NM_004380 4758055 AAUAGUAACUCUGGCCAUA L-003479-00 J-003479-10 CSNK1E 1454 NM_001894 40549399 CCACCAAGCGCCAGAAGUA L-003479-00 J-003479-11 CSNK1E 1454 NM_001894 40549399 CCUCCGAAUUCUCAACAUA L-003479-00 J-003479-12 CSNK1E 1454 NM_001894 40549399 CGACUACUCUUACCUACGU L-003479-00 J-003479-13 CSNK1E 1454 NM_001894 40549399 GAUCAGCCGCAUCGAGUAU
NCI-H2122 (continued)
L-009269-01 J-009269-09 CYP24A1 1591 NM_000782 55770849 GUGCAUUGGUCGCCGAUUA L-009269-01 J-009269-10 CYP24A1 1591 NM_000782 55770849 UCGAAGACUUGUACAGCGA L-009269-01 J-009269-11 CYP24A1 1591 NM_000782 55770849 UCUACAAUUUAUCCCGUAA L-009269-01 J-009269-12 CYP24A1 1591 NM_000782 55770849 GUGAAUAUGCUUUACCCAA L-003768-01 J-003768-09 DHX32 55760 NM_018180 20336299 GAAAAGUGGUGUCGUGAUU L-003768-01 J-003768-10 DHX32 55760 NM_018180 20336299 CCAUAUUCAUCACGUUAUU L-003768-01 J-003768-11 DHX32 55760 NM_018180 20336299 GGGAGAUACAUGACGCGAA L-003768-01 J-003768-12 DHX32 55760 NM_018180 20336299 CGUGAUUGUUUCAGGAGAU L-012029-00 J-012029-05 DIAPH2 1730 NM_007309 6382070 GAAGAUACUCAACGAAUUA L-012029-00 J-012029-06 DIAPH2 1730 NM_007309 6382070 GAAAUAACAGGGAACGAUU L-012029-00 J-012029-07 DIAPH2 1730 NM_007309 6382070 GAUCAGAGAUACUAAAUCA L-012029-00 J-012029-08 DIAPH2 1730 NM_007309 6382070 CGAUUUAACUCAUCUGAUA L-012423-00 J-012423-05 FBXL3P 26223 NM_012159 16306585 UUAGAGAACUGGCGUUGAA L-012423-00 J-012423-06 FBXL3P 26223 NM_012159 16306585 CAACUGUCCUCGACUGAUU L-012423-00 J-012423-07 FBXL3P 26223 NM_012159 16306585 GGGAUGCACUUAUUAAACA L-012423-00 J-012423-08 FBXL3P 26223 NM_012159 16306585 CAAGCGAGACUCAUGUUAA L-003134-00 J-003134-12 FGFR4 2264 NM_022963 47524176 CCUCGAAUAGGCACAGUUA L-003134-00 J-003134-13 FGFR4 2264 NM_022963 47524176 AUAACUACCUGCUAGAUGU L-003134-00 J-003134-14 FGFR4 2264 NM_022963 47524176 GCACGAGGCUCCAUGAUCG L-003134-00 J-003134-15 FGFR4 2264 NM_022963 47524176 GAUUACAGGUGACUCCUUG L-011043-00 J-011043-05 GLI3 2737 NM_000168 13518031 GCAUAUGACUUCCGCCUUA L-011043-00 J-011043-06 GLI3 2737 NM_000168 13518031 GAGCUUAGGUUCAGCCUUU L-011043-00 J-011043-07 GLI3 2737 NM_000168 13518031 GAACAGAUGUGAGCGAGAA L-011043-00 J-011043-08 GLI3 2737 NM_000168 13518031 GCAAUAGGCUUUAGGAAAA
NCI-H2122 (continued)
L-009498-01 J-009498-09 HEPH 9843 NM_014799 21166383 CUUACUGGAAAGUGCGAUA L-009498-01 J-009498-10 HEPH 9843 NM_014799 21166383 CAGAAGAGCUCCAGCCGAA L-009498-01 J-009498-11 HEPH 9843 NM_014799 21166383 GAGAAAGCUACGACGCAAU L-009498-01 J-009498-12 HEPH 9843 NM_014799 21166383 CCUGGUACCUGGUUAAUUA L-005069-00 J-005069-08 KRAS2 3845 NM_004985 34485723 GGAGGGCUUUCUUUGUGUA L-005069-00 J-005069-09 KRAS2 3845 NM_004985 34485723 UCAAAGACAAAGUGUGUAA L-005069-00 J-005069-10 KRAS2 3845 NM_004985 34485723 GAAGUUAUGGAAUUCCUUU L-005069-00 J-005069-11 KRAS2 3845 NM_004985 34485723 GAGAUAACACGAUGCGUAU L-019113-00 J-019113-05 NALP10/NLRP10 338322 NM_176821 78191799 CGCAGGAUCUGAAGCAUUU L-019113-00 J-019113-06 NALP10/NLRP10 338322 NM_176821 78191799 GCUCCUAUUUCACGGAUGA L-019113-00 J-019113-07 NALP10/NLRP10 338322 NM_176821 78191799 GAGAGGCAAAGUUGUCUUA L-019113-00 J-019113-08 NALP10/NLRP10 338322 NM_176821 78191799 GGAGGGCAAAGAUAAUAUA L-014165-00 J-014165-05 NYX 60506 NM_022567 12007645 GAGCUGAACCUCACCACGU L-014165-00 J-014165-06 NYX 60506 NM_022567 12007645 GGAUGUUGGUCCUGCUUCU L-014165-00 J-014165-07 NYX 60506 NM_022567 12007645 GCAUCGCCUUCGUGGAGGA L-014165-00 J-014165-08 NYX 60506 NM_022567 12007645 UCCAGAACCUCUCGGGUCU L-006656-00 J-006656-05 PARP1 142 NM_001618 11496989 GAUUUCAUCUGGUGUGAUA L-006656-00 J-006656-06 PARP1 142 NM_001618 11496989 GAAAACAGGUAUUGGAUAU L-006656-00 J-006656-07 PARP1 142 NM_001618 11496989 GUUCUUAGCGCACAUCUUG L-006656-00 J-006656-08 PARP1 142 NM_001618 11496989 CCAAUAGGCUUAAUCCUGU L-006770-00 J-006770-06 PI4KII/PIAK2A 55361 NM_018425 19923532 GGUUGGUGGUGCUGGAUUA L-006770-00 J-006770-07 PI4KII/PIAK2A 55361 NM_018425 19923532 CAACACUGAUCGAGGCAAU L-006770-00 J-006770-08 PI4KII/PIAK2A 55361 NM_018425 19923532 GAGACGAGCCCACUAGUGU L-006770-00 J-006770-09 PI4KII/PIAK2A 55361 NM_018425 19923532 GCAUCGGGCUACCACCAAA
NCI-H2122 (continued)
L-017555-01 J-017555-09 PIGC 5279 NM_153747 24430185 AGGUCUUGUAUGAGCGACA L-017555-01 J-017555-10 PIGC 5279 NM_153747 24430185 AAACAUCCAUGCUCGGAAA L-017555-01 J-017555-11 PIGC 5279 NM_153747 24430185 CAAUAUUGGGCUGUGGUAU L-017555-01 J-017555-12 PIGC 5279 NM_153747 24430185 CCUCCUAACUAGUAUAGAA L-008344-01 J-008344-09 RASL10B 91608 NM_033315 28274693 AGGUUGAGCUCUCCGGCUU L-008344-01 J-008344-10 RASL10B 91608 NM_033315 28274693 CCAUUGAGCUGUUACCCGA L-008344-01 J-008344-11 RASL10B 91608 NM_033315 28274693 GAGACGAGGGUGAUCGGAA L-008344-01 J-008344-12 RASL10B 91608 NM_033315 28274693 UGUCGCACCUGGUACGCAA L-019962-01 J-019962-09 STAG3 10734 NM_012447 57863309 GCGCAAGACCCAAGCCGAU L-019962-01 J-019962-10 STAG3 10734 NM_012447 57863309 CCUUCUUUGUGGAGAGCGA L-019962-01 J-019962-11 STAG3 10734 NM_012447 57863309 GUUUGAAUCCACACGUUAA L-019962-01 J-019962-12 STAG3 10734 NM_012447 57863309 GGGUAGAGAAGCCGAGAGA L-005035-00 J-005035-07 STK11 6794 NM_000455 58530881 UGACUGUGGUGCCGUACUU L-005035-00 J-005035-08 STK11 6794 NM_000455 58530881 GCUCUUACGGCAAGGUGAA L-005035-00 J-005035-09 STK11 6794 NM_000455 58530881 UGAAAGGGAUGCUUGAGUA L-005035-00 J-005035-10 STK11 6794 NM_000455 58530881 GAAGAAGGAAAUUCAACUA L-014805-02 J-014805-17 FLJ22679/TMEM164 84187 NM_032227 93277067 CCACCAAGACCGUCAUCUA L-014805-02 J-014805-18 FLJ22679/TMEM164 84187 NM_032227 93277067 UGGCUGGGCCCUUGUGUAA L-014805-02 J-014805-19 FLJ22679/TMEM164 84187 NM_032227 93277067 AGACGAAGGAGGACGGUAG L-014805-02 J-014805-20 FLJ22679/TMEM164 84187 NM_032227 93277067 UGACCAUGACCCACGGGAA L-003329-00 J-003329-14 TP53 7157 NM_000546 8400737 GAAAUUUGCGUGUGGAGUA L-003329-00 J-003329-15 TP53 7157 NM_000546 8400737 GUGCAGCUGUGGGUUGAUU L-003329-00 J-003329-16 TP53 7157 NM_000546 8400737 GCAGUCAGAUCCUAGCGUC L-003329-00 J-003329-17 TP53 7157 NM_000546 8400737 GGAGAAUAUUUCACCCUUC
ON-TARGETplus SMARTpool siRNA library NCI-H2126
Duplex Pool Catalog Gene GI Catalog Gene Symbol GENE ID Sequence Number Accession Number Number L-005772-00 J-005772-06 ADAMTS20 80070 NM_175851 41281881 CAAGCCACCUAGCGUAAUA L-005772-00 J-005772-07 ADAMTS20 80070 NM_175851 41281881 GCAAGGAACUGUUAUUGAA L-005772-00 J-005772-08 ADAMTS20 80070 NM_175851 41281881 CAAGAACUGUCCCGAGUGA L-005772-00 J-005772-09 ADAMTS20 80070 NM_175851 41281881 GUACAGCUAUUCUGGACAA L-008453-01 J-008453-09 ADHFE1 137872 NM_144650 21389518 CGGAAAUUCUUAUUCGAUC L-008453-01 J-008453-10 ADHFE1 137872 NM_144650 21389518 AGAGUGGAACCAACGGAUU L-008453-01 J-008453-11 ADHFE1 137872 NM_144650 21389518 CCAGAUGUUUCCAGAGCGA L-008453-01 J-008453-12 ADHFE1 137872 NM_144650 21389518 UGUCAGAAAUCCCGAUGAU L-011317-00 J-011317-05 LAF4/AFF3 3899 NM_001025108 68348715 GAAGCAGCAUUGUCGUUUA L-011317-00 J-011317-06 LAF4/AFF3 3899 NM_001025108 68348715 CAGUAGAGCUCAUCAGGUA L-011317-00 J-011317-07 LAF4/AFF3 3899 NM_001025108 68348715 GAUGAUGGCACGUUUAAUU L-011317-00 J-011317-08 LAF4/AFF3 3899 NM_001025108 68348715 GUAAAGGCGUGAAGCAGAA L-010376-00 J-010376-06 ALDOA 226 NM_184041 34577109 CCUAUUAAGCGGAGGUGUU L-010376-00 J-010376-07 ALDOA 226 NM_184041 34577109 GGACAAAUGGCGAGACUAC L-010376-00 J-010376-08 ALDOA 226 NM_184041 34577109 GGAGGAGUAUGUCAAGCGA L-010376-00 J-010376-09 ALDOA 226 NM_184041 34577109 CAAGGAAAGUACACUCCGA L-010992-00 J-010992-05 ANK1 286 NM_020481 70780362 GAGUAGGGCUAGUGAAUGU L-010992-00 J-010992-06 ANK1 286 NM_020481 70780362 GUUAGGGACUUAAGAAUCU L-010992-00 J-010992-07 ANK1 286 NM_020481 70780362 GAGUGAACUUGGUGCUCUA L-010992-00 J-010992-08 ANK1 286 NM_020481 70780362 UAUCAUAGUCCGUGUUCAA
NCI-H2126 (continued)
L-003202-00 J-003202-19 ATR 545 NM_001184 20143978 GAGAAAGGAUUGUAGACUA L-003202-00 J-003202-20 ATR 545 NM_001184 20143978 GCAACUCGCCUAACAGAUA L-003202-00 J-003202-21 ATR 545 NM_001184 20143978 CCACGAAUGUUAACUCUAU L-003202-00 J-003202-22 ATR 545 NM_001184 20143978 CCGCUAAUCUUCUAACAUU L-019573-00 J-019573-11 AURKC 6795 NM_001015879 62865640 GGAAAUUUGGGAAUGUGUA L-019573-00 J-019573-12 AURKC 6795 NM_001015879 62865640 CAAGGUAGAUGUGAGGUUU L-019573-00 J-019573-13 AURKC 6795 NM_001015879 62865640 GCGCACAGCCACGAUAAUA L-019573-00 J-019573-14 AURKC 6795 NM_001015879 62865640 GGGAGAACAUAUGAUGAAA L-008251-00 J-008251-05 BAAT 570 NM_001701 41349433 CCUCAACGACCCACGAUUU L-008251-00 J-008251-06 BAAT 570 NM_001701 41349433 CCUUUCAGAUGGUGAGUUU L-008251-00 J-008251-07 BAAT 570 NM_001701 41349433 GACUAUCUAUGGCUAUUUA L-008251-00 J-008251-08 BAAT 570 NM_001701 41349433 GGUAGUCUCUGUAUGUCAA L-005798-00 J-005798-12 CXORF53/BRCC3 79184 NM_001018055 64762483 CAUAAUGGCUCAGUGUUUA L-005798-00 J-005798-13 CXORF53/BRCC3 79184 NM_001018055 64762483 CGUCAGAAUUGUUCACAUU L-005798-00 J-005798-14 CXORF53/BRCC3 79184 NM_001018055 64762483 GAAGGACCGAGUAGAAAUU L-005798-00 J-005798-15 CXORF53/BRCC3 79184 NM_001018055 64762483 GCAUAUACUGGAACUGAAA L-010210-00 J-010210-06 BRE 9577 NM_199191 40353760 GAGGAUAACUGACUUAAAA L-010210-00 J-010210-07 BRE 9577 NM_199191 40353760 CGUGGAAUAUGAUGCAGAA L-010210-00 J-010210-08 BRE 9577 NM_199191 40353760 GCAACAAUAUCACCAAUUC L-010210-00 J-010210-09 BRE 9577 NM_199191 40353760 UGAUUACGUUCCUCAAGUA L-032104-02 J-032104-17 FLJ45803/C11orf92 399948 NM_207429 51702209 GGACUUUAACCUACAAUAA L-032104-02 J-032104-18 FLJ45803/C11orf92 399948 NM_207429 51702209 GAGGGUAAGCUGUUAAUUA L-032104-02 J-032104-19 FLJ45803/C11orf92 399948 NM_207429 51702209 AGGAAGACAAUUAGGUGAA L-032104-02 J-032104-20 FLJ45803/C11orf92 399948 NM_207429 51702209 GCUUGUAAGAUAUGCGAAC
NCI-H2126 (continued)
L-003635-00 J-003635-05 CALCR 799 NM_001742 46361988 CCGGUGAGCUGCAAGAUUU L-003635-00 J-003635-06 CALCR 799 NM_001742 46361988 CCAAUGGGCCCAAUUCAAA L-003635-00 J-003635-07 CALCR 799 NM_001742 46361988 CCACCUGGUUGAAGUAGUA L-003635-00 J-003635-08 CALCR 799 NM_001742 46361988 CGAACCUGGUCCAACUAUA L-004536-00 J-004536-07 CAMK2G 818 NM_001222 26667190 CAGGAGAUCAUUAAGAUUA L-004536-00 J-004536-08 CAMK2G 818 NM_001222 26667190 GCUCGGAUAUGUCGACUUC L-004536-00 J-004536-09 CAMK2G 818 NM_001222 26667190 GUACACAACGCUACAGAUG L-004536-00 J-004536-10 CAMK2G 818 NM_001222 26667190 GGAGAGUGUUAACCACAUC L-011007-00 J-011007-08 CDKN2A 1029 NM_058195 47132605 GAUCAUCAGUCACCGAAGG L-011007-00 J-011007-09 CDKN2A 1029 NM_058195 47132605 AAACACCGCUUCUGCCUUU L-011007-00 J-011007-10 CDKN2A 1029 NM_058195 47132605 UAACGUAGAUAUAUGCCUU L-011007-00 J-011007-11 CDKN2A 1029 NM_058195 47132605 CAGAACCAAAGCUCAAAUA L-011618-00 J-011618-05 COL4A1 1282 NM_001845 45580690 CAAAGGUGUUGACGGCUUA L-011618-00 J-011618-06 COL4A1 1282 NM_001845 45580690 CGCAAACGCUUACAGCUUU L-011618-00 J-011618-07 COL4A1 1282 NM_001845 45580690 AGAGUUGCCUCAUCUGUGA L-011618-00 J-011618-08 COL4A1 1282 NM_001845 45580690 GGGCAUGCCUGGUAUUGGU L-007869-02 J-007869-17 CSMD3 114788 NM_052900 38045885 CGAAGUGGAUCCUCAGAAA L-007869-02 J-007869-18 CSMD3 114788 NM_052900 38045885 CGGAUAAGAUAUAUAGCUU L-007869-02 J-007869-19 CSMD3 114788 NM_052900 38045885 GGAUUAUAAAUUAGGGUCA L-007869-02 J-007869-20 CSMD3 114788 NM_052900 38045885 AAUAAUAGCAGAAGAACGA L-020815-01 J-020815-09 CXORF15 55787 NM_018360 8922939 GAAACAAUAAUUUGGCGUA L-020815-01 J-020815-10 CXORF15 55787 NM_018360 8922939 AUAAGGUGUUCAAACGUAA L-020815-01 J-020815-11 CXORF15 55787 NM_018360 8922939 GCAGAGAACUUCAGCGUCA L-020815-01 J-020815-12 CXORF15 55787 NM_018360 8922939 GAACAGGGAUUUAGCAACA
NCI-H2126 (continued)
L-036784-01 J-036784-17 LOC645090/CXorf30 645090 NM_001098843 209915564 CUGAAAAUAGAGUGCGUAA L-036784-01 J-036784-18 LOC645090/CXorf30 645090 NM_001098843 209915564 CAAGAAAUCCUGAGCCGUU L-036784-01 J-036784-19 LOC645090/CXorf30 645090 NM_001098843 209915564 CCAUAAUGUUGAUCGCUAC L-036784-01 J-036784-20 LOC645090/CXorf30 645090 NM_001098843 209915564 CCUUUUAACACAAACGGAA L-014634-02 J-014634-18 FLJ10178/CXorf57 55086 NM_018015 193804922 GAACACAACUUUAGCGAUA L-014634-02 J-014634-19 FLJ10178/CXorf57 55086 NM_018015 193804922 CUUCAGAAAUAGAGCGCAC L-014634-02 J-014634-20 FLJ10178/CXorf57 55086 NM_018015 193804922 GCUUGAACUCUCUCGUAUA L-014634-02 J-014634-21 FLJ10178/CXorf57 55086 NM_018015 193804922 CAUAGAGGCCAGCCGUAUA L-005498-00 J-005498-07 FY/DARC 2532 NM_002036 42822886 GGGCUACUGUGUCUGGUAU L-005498-00 J-005498-08 FY/DARC 2532 NM_002036 42822886 GGACUCUGCACCCUGAUAU L-005498-00 J-005498-09 FY/DARC 2532 NM_002036 42822886 UCUUGUUGCCAUUGGGUUU L-005498-00 J-005498-10 FY/DARC 2532 NM_002036 42822886 GCAGAAGCCCUGGCAAUUU L-021491-00 J-021491-05 DCN 1634 NM_133507 47419921 AGAUGAGGCUUCUGGGAUA L-021491-00 J-021491-06 DCN 1634 NM_133507 47419921 GCUGGACCGUUUCAACAGA L-021491-00 J-021491-07 DCN 1634 NM_133507 47419921 UCAAUGCCAUCUUCGAGUG L-021491-00 J-021491-08 DCN 1634 NM_133507 47419921 CGACUUCGAGCCCUCCCUA L-018018-01 J-018018-09 DDX51 317781 NM_175066 37059776 AGAGGUGGCUACCGGCUUA L-018018-01 J-018018-10 DDX51 317781 NM_175066 37059776 CUGUCAAGGAAGAGCGCAA L-018018-01 J-018018-11 DDX51 317781 NM_175066 37059776 CAGAAGGAAUGUCACCGAA L-018018-01 J-018018-12 DDX51 317781 NM_175066 37059776 UCGACGAGGCUGACCGGAU L-019393-00 J-019393-05 DET1 55070 NM_017996 33354282 GUAGUAACACUGCGAGUCA L-019393-00 J-019393-06 DET1 55070 NM_017996 33354282 GAUAAUGGAUCAUCAUGUU L-019393-00 J-019393-07 DET1 55070 NM_017996 33354282 CAAGUACACUAGUGAGGAU L-019393-00 J-019393-08 DET1 55070 NM_017996 33354282 GAACAGGAUGGUAGUGCAA
NCI-H2126 (continued)
L-006712-00 J-006712-06 DGKB 1607 NM_145695 22027633 GAGGAUAAGCUUGAGUUUA L-006712-00 J-006712-07 DGKB 1607 NM_145695 22027633 ACGAAGCCAUCGACGAAUA L-006712-00 J-006712-08 DGKB 1607 NM_145695 22027633 CGUCAGGUUUACAGUCUUU L-006712-00 J-006712-09 DGKB 1607 NM_145695 22027633 GCAACAAGUUUCCUCAUUC L-015861-01 J-015861-09 DNAJB11 51726 NM_016306 51317390 GGUGAUUGCCGGACGAGAU L-015861-01 J-015861-10 DNAJB11 51726 NM_016306 51317390 GAAGUGCAAUUGUCGGCAA L-015861-01 J-015861-11 DNAJB11 51726 NM_016306 51317390 GUACAUAUUUCCCGGGAUA L-015861-01 J-015861-12 DNAJB11 51726 NM_016306 51317390 GGAGGUGGUCUGCGACGAA L-003261-00 J-003261-10 E2F3 1871 NM_001949 12669913 CUUCAUGUGUAGUUGAUUA L-003261-00 J-003261-11 E2F3 1871 NM_001949 12669913 GAAACACACAGUCCAAUGA L-003261-00 J-003261-12 E2F3 1871 NM_001949 12669913 CCAUUGAGGUUUACUUAUG L-003261-00 J-003261-13 E2F3 1871 NM_001949 12669913 AAUCAAAGGUUAGCUUAUG L-017045-01 J-017045-09 EIF2S3 1968 NM_001415 83656782 CUAAAGAACAAUACGAGCA L-017045-01 J-017045-10 EIF2S3 1968 NM_001415 83656782 CCUUAGUAGGUAACGGUAA L-017045-01 J-017045-11 EIF2S3 1968 NM_001415 83656782 GGAAAUCCACAGUCGUCAA L-017045-01 J-017045-12 EIF2S3 1968 NM_001415 83656782 GCAGACAAGCCACAAUUAA L-003117-00 J-003117-10 EPHA3 2042 NM_182644 32967313 CCUCAAGCCUGACACUAUA L-003117-00 J-003117-11 EPHA3 2042 NM_182644 32967313 GUUAGAGGGUCUUGUGUCA L-003117-00 J-003117-12 EPHA3 2042 NM_182644 32967313 ACAAGGCAUUGGAUGGUAA L-003117-00 J-003117-13 EPHA3 2042 NM_182644 32967313 GGUGAAAUUUCGAGAGCAU L-003801-00 J-003801-06 ETV1 2115 NM_004956 64368770 GAACAGCCCUUUAAAUUCA L-003801-00 J-003801-07 ETV1 2115 NM_004956 64368770 CAACGAAGGCUACGUGUAU L-003801-00 J-003801-08 ETV1 2115 NM_004956 64368770 UCUCCAAACUCAACUCAUA L-003801-00 J-003801-09 ETV1 2115 NM_004956 64368770 GAGAAAUUGUAACGAGAAA
NCI-H2126 (continued)
L-005872-00 J-005872-05 F9 2158 NM_000133 10518507 GGAAAGAACUGUGAAUUAG L-005872-00 J-005872-06 F9 2158 NM_000133 10518507 GAGAUCAGCUUUAGUUCUU L-005872-00 J-005872-07 F9 2158 NM_000133 10518507 GGACGAACCCUUAGUGCUA L-005872-00 J-005872-08 F9 2158 NM_000133 10518507 UGUGGAGGCUCUAUCGUUA L-018099-02 J-018099-17 FLJ20716/FAM70A 55026 NM_001104545 157388934 GAUUAGAGGUAGCGCAAAU L-018099-02 J-018099-18 FLJ20716/FAM70A 55026 NM_001104545 157388934 GGAUGUAUUGUUCCGGAAA L-018099-02 J-018099-19 FLJ20716/FAM70A 55026 NM_001104545 157388934 CGGAGUUAUUCUCGGCUUU L-018099-02 J-018099-20 FLJ20716/FAM70A 55026 NM_001104545 157388934 UGAAACCACUCUACGCUAA L-005204-00 J-005204-06 FBXL12 54850 NM_017703 8923178 GAUGCGACCUAAAGUCAUG L-005204-00 J-005204-07 FBXL12 54850 NM_017703 8923178 CGGAGAAGAUCCUGUGUAA L-005204-00 J-005204-08 FBXL12 54850 NM_017703 8923178 AGACAGGGCUGGAUGCUGG L-005204-00 J-005204-09 FBXL12 54850 NM_017703 8923178 GAAGGUACAUGGCAUCCCG L-003889-00 J-003889-05 FGF3 2248 NM_005247 15451899 GAGCUGGGCUAUAAUACGU L-003889-00 J-003889-06 FGF3 2248 NM_005247 15451899 GGCGGUACCUGGCCAUGAA L-003889-00 J-003889-07 FGF3 2248 NM_005247 15451899 GCGCCGAGAGACUGUGGUA L-003889-00 J-003889-08 FGF3 2248 NM_005247 15451899 AGAAGCAGAGCCCGGAUAA L-003139-00 J-003139-09 FRK 2444 NM_002031 31657133 CCAUUUGAUUUGUCGUAUA L-003139-00 J-003139-10 FRK 2444 NM_002031 31657133 GAACAAUACCACUCCAGUA L-003139-00 J-003139-11 FRK 2444 NM_002031 31657133 GUUGGAAACUUGAAGACUA L-003139-00 J-003139-12 FRK 2444 NM_002031 31657133 GAAGCCAUUCGUAGUAAUA L-010632-00 J-010632-05 PDZK10/FRMPD4 9758 NM_014728 55741444 GAAUUAGCUUCGUCCCAAA L-010632-00 J-010632-06 PDZK10/FRMPD4 9758 NM_014728 55741444 GGAGGUCGAUAUUUAACAU L-010632-00 J-010632-07 PDZK10/FRMPD4 9758 NM_014728 55741444 CCAAGGCUCUUCAGUGGAU L-010632-00 J-010632-08 PDZK10/FRMPD4 9758 NM_014728 55741444 GAAAACCUCUCCCGCAUGU
NCI-H2126 (continued)
L-005241-00 J-005241-12 HDAC9 9734 NM_058177 17158040 UCUUGUAGCUGGUGGAGUU L-005241-00 J-005241-13 HDAC9 9734 NM_058177 17158040 GAGAAUUUCACCUGGCAUU L-005241-00 J-005241-14 HDAC9 9734 NM_058177 17158040 GAAGGUGCGGUCCAGGUUA L-005241-00 J-005241-15 HDAC9 9734 NM_058177 17158040 CAACUGGAAGUGUUACUGA L-007180-00 J-007180-09 HERC2 8924 NM_004667 67190865 GCACAGAGUAUCACAGGUA L-007180-00 J-007180-10 HERC2 8924 NM_004667 67190865 CGAUGAAGGUUUGGUAUUU L-007180-00 J-007180-11 HERC2 8924 NM_004667 67190865 GAUAAUACGACACAGCUAA L-007180-00 J-007180-12 HERC2 8924 NM_004667 67190865 GCAGAUGUGUGCUAAGAUG L-007770-02 J-007770-21 HES1 3280 NM_005524 8400709 ACGAAGAGCAAGAAUAAAU L-007770-02 J-007770-22 HES1 3280 NM_005524 8400709 AGGCUGGAGAGGCGGCUAA L-007770-02 J-007770-23 HES1 3280 NM_005524 8400709 UCAACACGACACCGGAUAA L-007770-02 J-007770-24 HES1 3280 NM_005524 8400709 ACUGCAUGACCCAGAUCAA L-019092-00 J-019092-05 ZNFN1A1/IKZF1 10320 NM_006060 31657112 GGACGCACUCCGUUGGUAA L-019092-00 J-019092-06 ZNFN1A1/IKZF1 10320 NM_006060 31657112 GCGCAGCGGUCUCAUCUAC L-019092-00 J-019092-07 ZNFN1A1/IKZF1 10320 NM_006060 31657112 CAAGUAACGUCGCCAAACG L-019092-00 J-019092-08 ZNFN1A1/IKZF1 10320 NM_006060 31657112 GCUCAUGGUUCACAAAAGA L-008505-00 J-008505-07 INPP1 3628 NM_002194 4755138 CUGCAGAGACGCAUACCUA L-008505-00 J-008505-08 INPP1 3628 NM_002194 4755138 GGUAGCAUCUGAAGCAUUA L-008505-00 J-008505-09 INPP1 3628 NM_002194 4755138 CCAAUGAGUUUACUAAUGA L-008505-00 J-008505-10 INPP1 3628 NM_002194 4755138 GCAAAGUCCUCAAUGGUAA L-011539-00 J-011539-05 INPP4B 8821 NM_003866 4504706 CGAUGAAAUUGGAAUGUUA L-011539-00 J-011539-06 INPP4B 8821 NM_003866 4504706 AAUGAUGUAUUGCCAGUUA L-011539-00 J-011539-07 INPP4B 8821 NM_003866 4504706 GAAGGAUUGUUAAGUACAU L-011539-00 J-011539-08 INPP4B 8821 NM_003866 4504706 ACGAGAACAUUACGUGGUA
NCI-H2126 (continued)
L-012417-00 J-012417-05 DDX26/INTS6 26512 NM_012141 11024693 GAAGAGCACUCGCAGAUUU L-012417-00 J-012417-06 DDX26/INTS6 26512 NM_012141 11024693 GGGAGCACCUAACCUAAUA L-012417-00 J-012417-07 DDX26/INTS6 26512 NM_012141 11024693 CCACGAAGGUUGCAUACAU L-012417-00 J-012417-08 DDX26/INTS6 26512 NM_012141 11024693 GAGCCGAUCACAUGGUUUA L-011480-00 J-011480-05 IRS4 8471 NM_003604 4504732 GAGAGUGACUACAUGUUUA L-011480-00 J-011480-06 IRS4 8471 NM_003604 4504732 UGAAACAGGUCGAAUCUAU L-011480-00 J-011480-07 IRS4 8471 NM_003604 4504732 UCUGAGUGCUGUAUGGAUA L-011480-00 J-011480-08 IRS4 8471 NM_003604 4504732 GGGAAGAUUCAGACUUUAU L-005189-00 J-005189-06 ITGA4 3676 NM_000885 67191026 GACAAGACCUGUAGUAAUU L-005189-00 J-005189-07 ITGA4 3676 NM_000885 67191026 GCAUAUAUAUUCAGCAUUG L-005189-00 J-005189-08 ITGA4 3676 NM_000885 67191026 CAGAGUGACUGUAGCAAUA L-005189-00 J-005189-09 ITGA4 3676 NM_000885 67191026 AGAGACAGUUGGAGUUAUA L-008005-00 J-008005-09 ITGA9 3680 NM_002207 52485940 GUGCAGAGAUGUUUCAUGU L-008005-00 J-008005-10 ITGA9 3680 NM_002207 52485940 AAAUACAGCCCUUCAGUGA L-008005-00 J-008005-11 ITGA9 3680 NM_002207 52485940 GAAGAAAGUCGUACUAUAG L-008005-00 J-008005-12 ITGA9 3680 NM_002207 52485940 GAUAGUCCCUCAGUACUCA L-027250-00 J-027250-05 ITIH5L 347365 NM_198510 38348335 CCAGAAUCCUGAUAUAUUA L-027250-00 J-027250-06 ITIH5L 347365 NM_198510 38348335 UUGGAGACGUGCAGAUUUA L-027250-00 J-027250-07 ITIH5L 347365 NM_198510 38348335 GCACAUUCCCAAAUACAAU L-027250-00 J-027250-08 ITIH5L 347365 NM_198510 38348335 CUACAUUGCAGAAGUCAAA L-003148-00 J-003148-09 KDR 3791 NM_002253 11321596 GGGCAUGUACUGACGAUUA L-003148-00 J-003148-10 KDR 3791 NM_002253 11321596 CUACAUUGUUCUUCCGAUA L-003148-00 J-003148-11 KDR 3791 NM_002253 11321596 GGAAAUCUCUUGCAAGCUA L-003148-00 J-003148-12 KDR 3791 NM_002253 11321596 GCGAUGGCCUCUUCUGUAA
NCI-H2126 (continued)
L-009495-00 J-009495-08 C20ORF23/KIF16B 55614 NM_024704 41327690 GAUGAGACAUGGACUGUAU L-009495-00 J-009495-09 C20ORF23/KIF16B 55614 NM_024704 41327690 GAGUACAGGCUGCAAUAUA L-009495-00 J-009495-10 C20ORF23/KIF16B 55614 NM_024704 41327690 CAAAGACGCCUUCAGGAUU L-009495-00 J-009495-11 C20ORF23/KIF16B 55614 NM_024704 41327690 CGAAACAUACCAUUUGUGA L-015577-00 J-015577-05 KLHL10 317719 NM_152467 22748976 GUACGAGCCUCUUCAUUUA L-015577-00 J-015577-06 KLHL10 317719 NM_152467 22748976 GAAUAGGCGUGAUUGCUUA L-015577-00 J-015577-07 KLHL10 317719 NM_152467 22748976 UCACUGAACUUAAGGAUAU L-015577-00 J-015577-08 KLHL10 317719 NM_152467 22748976 UCAAUAGUGUUAAGCGUUU L-005907-00 J-005907-05 KLK10 5655 NM_145888 22208983 GAACGGAUGAGCACGAUCU L-005907-00 J-005907-06 KLK10 5655 NM_145888 22208983 GCAAAUACAUGUCCUGGAU L-005907-00 J-005907-07 KLK10 5655 NM_145888 22208983 GGAGACCAGUGCCAGGUUG L-005907-00 J-005907-08 KLK10 5655 NM_145888 22208983 AGUCAUACGCUCCAACUGA L-003846-00 J-003846-05 KREMEN1 83999 NM_153379 24041013 CAGCCAAUGGUGCGGAUUA L-003846-00 J-003846-06 KREMEN1 83999 NM_153379 24041013 GCACAACUAUUGCAGAAAU L-003846-00 J-003846-07 KREMEN1 83999 NM_153379 24041013 GCACGAGGAUGGUGUCUAC L-003846-00 J-003846-08 KREMEN1 83999 NM_153379 24041013 GAUACCUGCUUGCCAGAUG L-003865-00 J-003865-09 LATS2 26524 NM_014572 18959199 GCACGCAUUUUACGAAUUC L-003865-00 J-003865-10 LATS2 26524 NM_014572 18959199 ACACUCACCUCGCCCAAUA L-003865-00 J-003865-11 LATS2 26524 NM_014572 18959199 AAUCAGAUAUUCCUUGUUG L-003865-00 J-003865-12 LATS2 26524 NM_014572 18959199 GAAGUGAACCGGCAAAUGC
NCI-H2126 (continued)
L-007164-00 J-007164-05 LNX2 222484 NM_153371 34222215 CCAAGUGGCUCUUCAUAAA L-007164-00 J-007164-06 LNX2 222484 NM_153371 34222215 GGACAUACAUUCUGCUACA L-007164-00 J-007164-07 LNX2 222484 NM_153371 34222215 CUUCAUAGCUGCCACGAUA L-007164-00 J-007164-08 LNX2 222484 NM_153371 34222215 CAACGAAACACCUUUGAUU L-009810-00 J-009810-05 LOX 4015 NM_002317 21264603 AGUCCUGGCUGUUAUGAUA L-009810-00 J-009810-06 LOX 4015 NM_002317 21264603 CCUGGAAACUAUAUCCUAA L-009810-00 J-009810-07 LOX 4015 NM_002317 21264603 CACAGGACAUCAUGCGUAU L-009810-00 J-009810-08 LOX 4015 NM_002317 21264603 CAAUUUCACCGUAUUAGAA L-003844-00 J-003844-05 LRP5 4041 NM_002335 4505018 CGUCAAAGCCAUCGACUAU L-003844-00 J-003844-06 LRP5 4041 NM_002335 4505018 CGUCAUGGGUGGUGUCUAU L-003844-00 J-003844-07 LRP5 4041 NM_002335 4505018 GGACGGACCUACGGAGGAU L-003844-00 J-003844-08 LRP5 4041 NM_002335 4505018 GUACAGGCCCUACAUCAUU L-003512-00 J-003512-20 MAPK14 1432 NM_139013 20986513 GGAAUUCAAUGAUGUGUAU L-003512-00 J-003512-21 MAPK14 1432 NM_139013 20986513 UCUCCGAGGUCUAAAGUAU L-003512-00 J-003512-22 MAPK14 1432 NM_139013 20986513 GUAAUCUAGCUGUGAAUGA L-003512-00 J-003512-23 MAPK14 1432 NM_139013 20986513 GUCCAUCAUUCAUGCGAAA L-017379-00 J-017379-09 MCM3AP 8888 NM_003906 33469915 GCUAGCAGCCUCCUGAAUA L-017379-00 J-017379-10 MCM3AP 8888 NM_003906 33469915 GAGAACCGAUCUGGACAAA L-017379-00 J-017379-11 MCM3AP 8888 NM_003906 33469915 GAACCGCACGCGUGGCAUA L-017379-00 J-017379-12 MCM3AP 8888 NM_003906 33469915 GGAAGUCGGUGUUUAUUAC L-027126-00 J-027126-05 THRAP2/MED13L 23389 NM_015335 47575843 UAAAGUAUCUGCACGUAAA L-027126-00 J-027126-06 THRAP2/MED13L 23389 NM_015335 47575843 GCAGAGAGGCGCUUAAUGA L-027126-00 J-027126-07 THRAP2/MED13L 23389 NM_015335 47575843 CUACACAGAUCCACAUUAA L-027126-00 J-027126-08 THRAP2/MED13L 23389 NM_015335 47575843 GCCCACAGCCUCUAUGUUA
NCI-H2126 (continued)
L-006951-00 J-006951-05 MYCBP2 23077 NM_015057 56118211 GGAAGUAGUUCUGUUAUUU L-006951-00 J-006951-06 MYCBP2 23077 NM_015057 56118211 GCAGAAAUGCCCACACUUU L-006951-00 J-006951-07 MYCBP2 23077 NM_015057 56118211 GAAUAGUUGUGGUUGGUUU L-006951-00 J-006951-08 MYCBP2 23077 NM_015057 56118211 GCAAGGAUUUGGUGUGUGU L-012182-02 J-012182-18 NAP1L2 4674 NM_021963 21614528 UAGAAGACAUUGAUCGUUA L-012182-02 J-012182-19 NAP1L2 4674 NM_021963 21614528 GGUUAUUGUUUGUCGGUGA L-012182-02 J-012182-20 NAP1L2 4674 NM_021963 21614528 CAAAAGGACUUAUCGGUUA L-012182-02 J-012182-21 NAP1L2 4674 NM_021963 21614528 GGUCACAAUUUACGUACUU L-026539-01 J-026539-09 KIAA0056/NCAPD3 23310 NM_015261 76880473 GAUUAACAGUCCUACGUUU L-026539-01 J-026539-10 KIAA0056/NCAPD3 23310 NM_015261 76880473 GAUGAGAAGACCAACGUUA L-026539-01 J-026539-11 KIAA0056/NCAPD3 23310 NM_015261 76880473 CAGGGAAUAUGGACGAAGA L-026539-01 J-026539-12 KIAA0056/NCAPD3 23310 NM_015261 76880473 GGGAAUACGCUAUGUUCAU L-014446-01 J-014446-09 FLJ12610/NHE11 79840 NM_024782 13376141 GGGCUACGCUGAUUCGAGA L-014446-01 J-014446-10 FLJ12610/NHE11 79840 NM_024782 13376141 GAGGGAGCUAGCAACGUUA L-014446-01 J-014446-11 FLJ12610/NHE11 79840 NM_024782 13376141 CCUUCAGAUUCUUCGUAAA L-014446-01 J-014446-12 FLJ12610/NHE11 79840 NM_024782 13376141 AGAAAGAGUCCACGGGUAC L-003161-00 J-003161-09 NTRK3 4916 NM_002530 59889559 UGUAGUUUCUGGCGGAUUU L-003161-00 J-003161-10 NTRK3 4916 NM_002530 59889559 ACGGACAUCUCAAGGAAUA L-003161-00 J-003161-11 NTRK3 4916 NM_002530 59889559 GGUCGACGGUCCAAAUUUG L-003161-00 J-003161-12 NTRK3 4916 NM_002530 59889559 GCAAGACUGAGAUCAAUUG L-017570-01 J-017570-09 NUP54 53371 NM_017426 26051236 GCAGAUCUGUUACGAGAAA L-017570-01 J-017570-10 NUP54 53371 NM_017426 26051236 AUAUAAAGCUGGUCGAACA L-017570-01 J-017570-11 NUP54 53371 NM_017426 26051236 AUGAAGAGCAGUUGCGAGU L-017570-01 J-017570-12 NUP54 53371 NM_017426 26051236 CAUCAUUAAAGACGAUCUA
NCI-H2126 (continued)
L-008445-01 J-008445-09 NXF3 56000 NM_022052 11545756 UGCACGACCUUCUCGGAUA L-008445-01 J-008445-10 NXF3 56000 NM_022052 11545756 GGAGAGAAGUGUGCGGACA L-008445-01 J-008445-11 NXF3 56000 NM_022052 11545756 GCAUCGAAUCCUAGAAAGU L-008445-01 J-008445-12 NXF3 56000 NM_022052 11545756 CGUAACAACCAGUGUGACU L-014165-00 J-014165-05 NYX 60506 NM_022567 12007645 GAGCUGAACCUCACCACGU L-014165-00 J-014165-06 NYX 60506 NM_022567 12007645 GGAUGUUGGUCCUGCUUCU L-014165-00 J-014165-07 NYX 60506 NM_022567 12007645 GCAUCGCCUUCGUGGAGGA L-014165-00 J-014165-08 NYX 60506 NM_022567 12007645 UCCAGAACCUCUCGGGUCU L-011745-00 J-011745-05 PAX7 5081 NM_013945 7524358 GCUUGUUUAUGGAGAGCUA L-011745-00 J-011745-06 PAX7 5081 NM_013945 7524358 ACAUGAACCCGGUCAGCAA L-011745-00 J-011745-07 PAX7 5081 NM_013945 7524358 GUGCAGGUCUGGUUCAGUA L-011745-00 J-011745-08 PAX7 5081 NM_013945 7524358 GAAUCAAGUUCGGGAAGAA L-020442-00 J-020442-05 PDZRN4 29951 NM_013377 39653318 GAUGAAAGCUUGCGAAAUG L-020442-00 J-020442-06 PDZRN4 29951 NM_013377 39653318 GAAAUCUGCAGUCGAGUAU L-020442-00 J-020442-07 PDZRN4 29951 NM_013377 39653318 GCGAACACCUCUUAGUAGA L-020442-00 J-020442-08 PDZRN4 29951 NM_013377 39653318 GGGAAGAUGUCCAGAAUCG L-010253-00 J-010253-10 PFKP 5214 NM_002627 41352062 GGAACGGCCAGAUCGAUAA L-010253-00 J-010253-11 PFKP 5214 NM_002627 41352062 GGGCCAAGGUGUACUUCAU L-010253-00 J-010253-12 PFKP 5214 NM_002627 41352062 GCAGAUGUGUGUCAAACUC L-010253-00 J-010253-13 PFKP 5214 NM_002627 41352062 GAAGUACGCCUACCUCAAC L-003433-00 J-003433-05 PGR 5241 NM_000926 31981491 GGACGUGGAGGGCGCAUAU L-003433-00 J-003433-06 PGR 5241 NM_000926 31981491 GAGAUGAGGUCAAGCUACA L-003433-00 J-003433-07 PGR 5241 NM_000926 31981491 ACAUAUUGAUGACCAGAUA L-003433-00 J-003433-08 PGR 5241 NM_000926 31981491 GCACCUGAUCUAAUACUAA
NCI-H2126 (continued)
L-004535-00 J-004535-09 PIP5K2C/PIP4K2C 79837 NM_024779 37059743 GAACCUCCGUGAUCGAUUU L-004535-00 J-004535-10 PIP5K2C/PIP4K2C 79837 NM_024779 37059743 GCAGUAUGCUAAGCGAUUC L-004535-00 J-004535-11 PIP5K2C/PIP4K2C 79837 NM_024779 37059743 GAAGAGAGAUGUGGAGUUU L-004535-00 J-004535-12 PIP5K2C/PIP4K2C 79837 NM_024779 37059743 GAAGAAAGCAGCUCAUGCA L-003325-00 J-003325-18 PLK2 10769 NM_006622 5730054 CCGGAGAUCUCGCGGAUUA L-003325-00 J-003325-19 PLK2 10769 NM_006622 5730054 AAGUGACGGUGCUGAAAUA L-003325-00 J-003325-20 PLK2 10769 NM_006622 5730054 GCUAGUAUGUUGUCCAAAA L-003325-00 J-003325-21 PLK2 10769 NM_006622 5730054 CAUCAAUGAGGAUAGGAUA L-007752-00 J-007752-05 PLXNB3 5365 NM_005393 10864080 CCAAACGGACCCUGAAUGA L-007752-00 J-007752-06 PLXNB3 5365 NM_005393 10864080 GCGGAACCUUCAACAUUUC L-007752-00 J-007752-07 PLXNB3 5365 NM_005393 10864080 CCCAUUACGUGCACAGGAA L-007752-00 J-007752-08 PLXNB3 5365 NM_005393 10864080 CAGAGUAGCCACUGCGUGU L-020132-00 J-020132-05 POLE 5426 NM_006231 62198236 GCGAGGAACAGGCGAAAUA L-020132-00 J-020132-06 POLE 5426 NM_006231 62198236 GGAGGAGGGUGCUUCGUAU L-020132-00 J-020132-07 POLE 5426 NM_006231 62198236 GGACAGGCGUUACGAGUUC L-020132-00 J-020132-08 POLE 5426 NM_006231 62198236 CUCGGAAGCUGGAAGAUUA L-004197-00 J-004197-09 PRKD2 25865 NM_016457 48255892 GCUGAAGAGCUGAGCCGUA L-004197-00 J-004197-10 PRKD2 25865 NM_016457 48255892 CGACCAACAGAUACUAUAA L-004197-00 J-004197-11 PRKD2 25865 NM_016457 48255892 GGAAGAUGGGAGAGCGAUA L-004197-00 J-004197-12 PRKD2 25865 NM_016457 48255892 CAAUGGAGAUGUGCCGAUG L-008063-00 J-008063-05 PTCH2 8643 NM_003738 52145304 CAGUGGAACUCUUUGGUAU L-008063-00 J-008063-06 PTCH2 8643 NM_003738 52145304 GUACUCACAUCCAUCAACA L-008063-00 J-008063-07 PTCH2 8643 NM_003738 52145304 CACCCGAGCUCUUCUACAU L-008063-00 J-008063-08 PTCH2 8643 NM_003738 52145304 GGUCUCCGCAUGGCCAUUA
NCI-H2126 (continued)
L-008832-00 J-008832-05 PTPN9 5780 NM_002833 18375663 GAAAACAACGCUAGAAAUU L-008832-00 J-008832-06 PTPN9 5780 NM_002833 18375663 GGACAGUUCAGUACAAUGU L-008832-00 J-008832-07 PTPN9 5780 NM_002833 18375663 GUCAGUAACUCUCCUACGA L-008832-00 J-008832-08 PTPN9 5780 NM_002833 18375663 AAAUUGAUCUCGCCACUUG L-008500-00 J-008500-06 PTPRO 5800 NM_030668 13677215 GCCAAAGACUCUGACUAUA L-008500-00 J-008500-07 PTPRO 5800 NM_030668 13677215 GGUCAUACCGGAUGUCUAU L-008500-00 J-008500-08 PTPRO 5800 NM_030668 13677215 UCAGUGAUGUCAUAUACGA L-008500-00 J-008500-09 PTPRO 5800 NM_030668 13677215 CAAACAUCCUACCAUAUGA L-009052-00 J-009052-05 RAP2B 5912 NM_002886 38201689 UAAAGUAGAUCCAAAGUGU L-009052-00 J-009052-06 RAP2B 5912 NM_002886 38201689 GGGAAAGUUUGCUAAUAUA L-009052-00 J-009052-07 RAP2B 5912 NM_002886 38201689 CAAUAGGGCCUGUUGUUUA L-009052-00 J-009052-08 RAP2B 5912 NM_002886 38201689 GAAAUUAGGAACACUGCUA L-003437-00 J-003437-06 RARA 5914 NM_001033603 75812907 GCAAAUACACUACGAACAA L-003437-00 J-003437-07 RARA 5914 NM_001033603 75812907 CCAAGGAGUCUGUGAGAAA L-003437-00 J-003437-08 RARA 5914 NM_001033603 75812907 GAGCAGCAGUUCUGAAGAG L-003437-00 J-003437-09 RARA 5914 NM_001033603 75812907 GAACAACGUGUCUCUCUGG L-004333-00 J-004333-05 RASA2 5922 NM_006506 12545407 GCAAAUAACUGUGUAGAAG L-004333-00 J-004333-06 RASA2 5922 NM_006506 12545407 AAACGAUGGUUCUGCUUAA L-004333-00 J-004333-07 RASA2 5922 NM_006506 12545407 AUUCAGAGGUUCAGGGUAA L-004333-00 J-004333-08 RASA2 5922 NM_006506 12545407 ACUAAAGAGUCCAGUGGUA L-009117-00 J-009117-05 RASA3 22821 NM_007368 38201691 AAAGAAGGGUUCAUGAUCA L-009117-00 J-009117-06 RASA3 22821 NM_007368 38201691 CCGGAGGGAUUCCAUCAUA L-009117-00 J-009117-07 RASA3 22821 NM_007368 38201691 GAUCGACAUUCUCACCAAA L-009117-00 J-009117-08 RASA3 22821 NM_007368 38201691 UGAAAUUCCUCGGAGCUUU
NCI-H2126 (continued)
L-008826-00 J-008826-05 RGS14 10636 NM_006480 56682941 CCACCUAGAACUCAGGAUA L-008826-00 J-008826-06 RGS14 10636 NM_006480 56682941 GGAAAGAGGACCUGGUACU L-008826-00 J-008826-07 RGS14 10636 NM_006480 56682941 GCAGGGAUCUGUGAGAAAC L-008826-00 J-008826-08 RGS14 10636 NM_006480 56682941 UGAUGAAGUUCGACAGCUA L-004162-00 J-004162-06 SGKL/SGK3 23678 NM_170709 25168266 GAAAGCUGCCCAAGUGUAA L-004162-00 J-004162-07 SGKL/SGK3 23678 NM_170709 25168266 GGAGAUAUGCAGAGUUUGA L-004162-00 J-004162-08 SGKL/SGK3 23678 NM_170709 25168266 GUGUGUAUCUUCUGACUAU L-004162-00 J-004162-09 SGKL/SGK3 23678 NM_170709 25168266 GCAUUGGGUUACUUACAUU L-003959-00 J-003959-14 SNF1LK/SIK1 150094 NM_173354 48762713 ACGAUUAGAUUCAAGCAAU L-003959-00 J-003959-15 SNF1LK/SIK1 150094 NM_173354 48762713 GGAGUACUGUCACGACCAU L-003959-00 J-003959-16 SNF1LK/SIK1 150094 NM_173354 48762713 GAAAAUCUAUCGUGAGGUU L-003959-00 J-003959-17 SNF1LK/SIK1 150094 NM_173354 48762713 GCUCGGACCUCAGUGGUUU L-010431-00 J-010431-05 SMARCA4 6597 NM_003072 21071055 GCACACCGCUGCAGAACAA L-010431-00 J-010431-06 SMARCA4 6597 NM_003072 21071055 CCAAGCCGGUCGUGAGUGA L-010431-00 J-010431-07 SMARCA4 6597 NM_003072 21071055 GCGACUCACUGACGGAGAA L-010431-00 J-010431-08 SMARCA4 6597 NM_003072 21071055 GACCAGCACUCCCAAGGUU L-006836-01 J-006836-09 SMC2L1/SMC2 10592 NM_006444 5453590 GCAGAGGCUCAGCGAGUUA L-006836-01 J-006836-10 SMC2L1/SMC2 10592 NM_006444 5453590 GGUUCGGGCUUCUAAUUUA L-006836-01 J-006836-11 SMC2L1/SMC2 10592 NM_006444 5453590 CAGCAAAGCUCAUAUCACA L-006836-01 J-006836-12 SMC2L1/SMC2 10592 NM_006444 5453590 CCCAAGACACUGUAAUUAA L-022738-01 J-022738-09 SMYD1 150572 NM_198274 58037544 GUGAUUAACUGCAACGGUU L-022738-01 J-022738-10 SMYD1 150572 NM_198274 58037544 AAGAAUGAAUGUUCGGCCA L-022738-01 J-022738-11 SMYD1 150572 NM_198274 58037544 GUAUCAUGAGGUUGUGAAA L-022738-01 J-022738-12 SMYD1 150572 NM_198274 58037544 GCCCAACUGUACUGUCAUA
NCI-H2126 (continued)
L-005035-00 J-005035-07 STK11 6794 NM_000455 58530881 UGACUGUGGUGCCGUACUU L-005035-00 J-005035-08 STK11 6794 NM_000455 58530881 GCUCUUACGGCAAGGUGAA L-005035-00 J-005035-09 STK11 6794 NM_000455 58530881 UGAAAGGGAUGCUUGAGUA L-005035-00 J-005035-10 STK11 6794 NM_000455 58530881 GAAGAAGGAAAUUCAACUA L-005385-00 J-005385-10 TAF1L 138474 NM_153809 24429571 CCAAGCAACUUCUACGUAA L-005385-00 J-005385-11 TAF1L 138474 NM_153809 24429571 GAUCAGAGCUAAGGUGUCA L-005385-00 J-005385-12 TAF1L 138474 NM_153809 24429571 GAUGAAGGGUGGAUUAGGA L-005385-00 J-005385-13 TAF1L 138474 NM_153809 24429571 CUAGGAAUGUAAUGGCUUA L-004846-00 J-004846-07 KIAA1361/TAOK1 57551 NM_020791 45439369 CCAAGUAUCUCGUCACAAA L-004846-00 J-004846-08 KIAA1361/TAOK1 57551 NM_020791 45439369 GUAAUAUGGUCCUUUCUAA L-004846-00 J-004846-09 KIAA1361/TAOK1 57551 NM_020791 45439369 CUAAAGUGAUGUCCAAUGA L-004846-00 J-004846-10 KIAA1361/TAOK1 57551 NM_020791 45439369 GCUGUGAGUUGAUCAGAUU L-024781-02 J-024781-17 LOC340543/TCEAL5 340543 NM_001012979 61175238 GUACAGAAGAUGAAGGAAA L-024781-02 J-024781-18 LOC340543/TCEAL5 340543 NM_001012979 61175238 ACCUAGAAAGUGAGGGAAA L-024781-02 J-024781-19 LOC340543/TCEAL5 340543 NM_001012979 61175238 AAGAUGUCCCAUAUGUUUA L-024781-02 J-024781-20 LOC340543/TCEAL5 340543 NM_001012979 61175238 AGGAAGAAAAGCCGGACAU L-021352-02 J-021352-17 TMEM47 83604 NM_031442 169234733 UAAGAAAGGAAUAGCGAUA L-021352-02 J-021352-18 TMEM47 83604 NM_031442 169234733 GAAGUUAACUGAUGGGCUA L-021352-02 J-021352-19 TMEM47 83604 NM_031442 169234733 CUACAUUGGCUGUUGGUUA L-021352-02 J-021352-20 TMEM47 83604 NM_031442 169234733 UCUCGAAGGCGUUUCUAUA L-003329-00 J-003329-14 TP53 7157 NM_000546 8400737 GAAAUUUGCGUGUGGAGUA L-003329-00 J-003329-15 TP53 7157 NM_000546 8400737 GUGCAGCUGUGGGUUGAUU L-003329-00 J-003329-16 TP53 7157 NM_000546 8400737 GCAGUCAGAUCCUAGCGUC L-003329-00 J-003329-17 TP53 7157 NM_000546 8400737 GGAGAAUAUUUCACCCUUC
NCI-H2126 (continued)
L-010548-00 J-010548-06 TPR 7175 NM_003292 4507658 GAAGAAGUGCGUAAGAAUA L-010548-00 J-010548-07 TPR 7175 NM_003292 4507658 UCAGUUGACUCCAGGAAUA L-010548-00 J-010548-08 TPR 7175 NM_003292 4507658 UCAAGGAGGUUUAGGAAUG L-010548-00 J-010548-09 TPR 7175 NM_003292 4507658 GGCAUACACUUACUAGAAA L-006538-00 J-006538-06 TRIM37 4591 NM_001005207 52487175 GGACUUUGCUGGAGGUUAA L-006538-00 J-006538-07 TRIM37 4591 NM_001005207 52487175 AUACGAAACUCCACAAAUA L-006538-00 J-006538-08 TRIM37 4591 NM_001005207 52487175 AGAGUGAGUUGAUAUCUAA L-006538-00 J-006538-09 TRIM37 4591 NM_001005207 52487175 GAAUGUAGAAGCUGUAAGA L-005048-00 J-005048-06 TRPM6 140803 NM_017662 18921092 GAGAGGAGGCCACGGUUAA L-005048-00 J-005048-07 TRPM6 140803 NM_017662 18921092 CGAAUAACCUUGAUUGACA L-005048-00 J-005048-08 TRPM6 140803 NM_017662 18921092 CUAAUACACUCUUGCAUCA L-005048-00 J-005048-09 TRPM6 140803 NM_017662 18921092 GCUGAAGGGCACAAAUUUA L-005395-00 J-005395-05 TTN 7273 NM_133379 20143915 GUGGAGACAUUACAUAUUA L-005395-00 J-005395-06 TTN 7273 NM_133379 20143915 GGUACUCAAUCACUAAUUA L-005395-00 J-005395-07 TTN 7273 NM_133379 20143915 GAAUUGAUGUCCUGUGGAA L-005395-00 J-005395-08 TTN 7273 NM_133379 20143915 UGUGGAAGCUCGUCAUAUA L-021392-00 J-021392-05 UNC5B 219699 NM_170744 32261317 GACCUUAUGCCUUCAAGAU L-021392-00 J-021392-06 UNC5B 219699 NM_170744 32261317 GCCCGUGACUGGAUCUUUC L-021392-00 J-021392-07 UNC5B 219699 NM_170744 32261317 GCGGAUACUUGGUGGAGGA L-021392-00 J-021392-08 UNC5B 219699 NM_170744 32261317 GCACAUACCCUAGCGAUUU L-022513-00 J-022513-06 USP31 57478 NM_020718 42415502 ACAAUAAUCAGAUCGCUUA L-022513-00 J-022513-07 USP31 57478 NM_020718 42415502 GAACUCCAGUGUCGUAGAU L-022513-00 J-022513-08 USP31 57478 NM_020718 42415502 UGGCUUGGAUUAUCAUAGA L-022513-00 J-022513-09 USP31 57478 NM_020718 42415502 UGAGACUCCCGAAAUAUUU
NCI-H2126 (continued)
L-006091-00 J-006091-05 USP44 84101 NM_032147 14149816 GAAUUGGAGUAUCAAGUUA L-006091-00 J-006091-06 USP44 84101 NM_032147 14149816 GAAGGAUACUAAUGGGUAA L-006091-00 J-006091-07 USP44 84101 NM_032147 14149816 CAAGAAAGAGUUUACGUUU L-006091-00 J-006091-08 USP44 84101 NM_032147 14149816 AAUAGUAACUCCUGGUGUA L-003551-00 J-003551-06 VIM 7431 NM_003380 62414288 UCACGAUGACCUUGAAUAA L-003551-00 J-003551-07 VIM 7431 NM_003380 62414288 GAGGGAAACUAAUCUGGAU L-003551-00 J-003551-08 VIM 7431 NM_003380 62414288 UUAAGACGGUUGAAACUAG L-003551-00 J-003551-09 VIM 7431 NM_003380 62414288 GGAAAUGGCUCGUCACCUU L-014860-00 J-014860-05 MORG1/WDR83 84292 NM_032332 39725650 UGAACACGGUGCAGUUUAA L-014860-00 J-014860-06 MORG1/WDR83 84292 NM_032332 39725650 GGGCAGGUCGUGCGCAAAU L-014860-00 J-014860-07 MORG1/WDR83 84292 NM_032332 39725650 GUGCGAGCCGUACGAUUUA L-014860-00 J-014860-08 MORG1/WDR83 84292 NM_032332 39725650 GUGAGACGCUAUGACCUAA L-005362-00 J-005362-05 PRKWNK1/WNK1 65125 NM_018979 12711659 GCAGUUGUCUCAAUAUCUA L-005362-00 J-005362-06 PRKWNK1/WNK1 65125 NM_018979 12711659 GCAGGAGUGUCUAGUUAUA L-005362-00 J-005362-07 PRKWNK1/WNK1 65125 NM_018979 12711659 UAAAGGAGACCGAGUAGUA L-005362-00 J-005362-08 PRKWNK1/WNK1 65125 NM_018979 12711659 GAGAGUCGUUUGUGGAUCA L-013957-01 J-013957-09 ZNF521 25925 NM_015461 24308068 AGAAAGUGCCAUCGUGAAA L-013957-01 J-013957-10 ZNF521 25925 NM_015461 24308068 GUGAAGCACAGUAACGAAA L-013957-01 J-013957-11 ZNF521 25925 NM_015461 24308068 GAACACAAAGUCACGCAUA L-013957-01 J-013957-12 ZNF521 25925 NM_015461 24308068 UGACUGGUCCCUCGAGUAA
Control siRNA (Dharmacon) Catalog number Name D-001810-01-05 Non-targeting siRNA#1 D-001810-04-05 Non-targeting siRNA#2 L-003317-01-0005 KIF11 (SMARTpool)
MAP3K9 siRNA Oligo 1 Ambion Life Technologies (Silencer Select pre-designed s8806) Sequence: GACCAUCUUUUCACGAAUAUtt Oligo 2 Santa%Cruz%Biotechnology%(sc539110) Pool of 3 target-specific 20-25 nucleotide siRNA, sequence not available Oligo 3 ORIGENE%(SR302910B) Sequence: ACCUUUGAAUAGAGUGUUAUCUGGG