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Pathogen-Triggered Programmed Cell Death in Plants: Metacaspase 1-Dependent Pathways

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Pathogen-Triggered Programmed Cell Death in Plants: Metacaspase 1-Dependent Pathways Pathogen-triggered programmed cell death in plants: Metacaspase 1-dependent pathways Muerte celular programada desencadenada por patógenos en plantas: vías dependientes de Metacaspasa 1 Saúl Stalin Lema Asqui ADVERTIMENT. La consulta d’aquesta tesi queda condicionada a l’acceptació de les següents condicions d'ús: La difusió d’aquesta tesi per mitjà del servei TDX (www.tdx.cat) i a través del Dipòsit Digital de la UB (diposit.ub.edu) ha estat autoritzada pels titulars dels drets de propietat intel·lectual únicament per a usos privats emmarcats en activitats d’investigació i docència. No s’autoritza la seva reproducció amb finalitats de lucre ni la seva difusió i posada a disposició des d’un lloc aliè al servei TDX ni al Dipòsit Digital de la UB. No s’autoritza la presentació del seu contingut en una finestra o marc aliè a TDX o al Dipòsit Digital de la UB (framing). Aquesta reserva de drets afecta tant al resum de presentació de la tesi com als seus continguts. En la utilització o cita de parts de la tesi és obligat indicar el nom de la persona autora. ADVERTENCIA. La consulta de esta tesis queda condicionada a la aceptación de las siguientes condiciones de uso: La difusión de esta tesis por medio del servicio TDR (www.tdx.cat) y a través del Repositorio Digital de la UB (diposit.ub.edu) ha sido autorizada por los titulares de los derechos de propiedad intelectual únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con finalidades de lucro ni su difusión y puesta a disposición desde un sitio ajeno al servicio TDR o al Repositorio Digital de la UB. No se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR o al Repositorio Digital de la UB (framing). Esta reserva de derechos afecta tanto al resumen de presentación de la tesis como a sus contenidos. En la utilización o cita de partes de la tesis es obligado indicar el nombre de la persona autora. WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tdx.cat) service and by the UB Digital Repository (diposit.ub.edu) has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reproduction with lucrative aims is not authorized nor its spreading and availability from a site foreign to the TDX service or to the UB Digital Repository. Introducing its content in a window or frame foreign to the TDX service or to the UB Digital Repository is not authorized (framing). Those rights affect to the presentation summary of the thesis as well as to its contents. In the using or citation of parts of the thesis it’s obliged to indicate the name of the author. Facultat de Biologia Departament de Genètica, microbiologia i estadística Programa de doctorat: Genètica “Pathogen-triggered programmed cell death in plants: Metacaspase 1-dependent pathways” (Muerte celular programada desencadenada por patógenos en plantas: Vías dependientes de Metacaspasa 1.) Memòria presentada per Saúl Stalin Lema Asqui per tal d´optar al títol de Doctor per la Universitat de Barcelona. Tesi doctoral realitzada sota la direcció de la Dra. Núria Sánchez Coll i del Dr. Marc Valls Matheu al Departament de Genètica, Microbiologia i Estadística de la Facultat de Biologia (UB) i al Centre de Recerca Agrigenòmica (CRAG). Signatura dels directors, Signatura del tutor Dra. Núria Sánchez Coll. Dr. Marc Valls i Matheu Dr. Marc Valls i Matheu Signatura del doctorand, Saúl S. Lema Asqui BARCELONA, JUNE 2018 ACKNOWLEDGMENTS III Más de una década me ha tomado llegar hasta este punto, y que por mucho que se me hubiese permitido soñar jamás me lo hubiese imaginado, aunque el camino ha sido largo y a veces lleno de peripecias, que sin el apoyo de cada una de las personas, que de una u otro forma han aportado con palabras, gestos, caricias y uno que otro golpe para guiarme; y ver realizado uno de los momentos mas esperados en mi vida. Nunca he sido un buen escritor y el escribir estas palabras ha resultado el momento mas duro de toda mi vida, porque quisiera transmitir con ellas toda la gratitud que tengo para con todos ustedes que me han acompañado a lo largo de esta aventura. Primeramente, quiero agradecer de todo corazón a mi familia que han sabido entenderme y apoyarme a lo largo de todo este tiempo, a mi madre Inés que ha sido el pilar fundamental de mi carrera y fundamentalmente de mi vida; que sepas que siempre, aunque nunca lo parezca escucho tus consejos; a mi hermana Karina a la cual extraño demasiado y siento haberme perdido gran parte de tu vida ñaña, gracias por haberme apoyado siempre y nunca haber dudado que podría conseguir lo que me propusiese, a mi padre Saúl que siempre ha sabido encontrar las palabras adecuadas para reconfortarme en los momentos mas difíciles. A ti Daniela que has sido mi apoyo incondicional durante este tiempo, sin tu presencia, apoyo, compresión y amor este logro no sería lo mismo, gracias por estar conmigo incluso en los momentos que te volvía loco; a toda mi familia en Ecuador a mis abuelitas, a mis tíos y primos que nunca dudaron que podría ser capaz de conseguir lo que me propusiera; volar como siempre me lo decían, millón de gracias por su apoyo a la distancia. A la Dra. Núria Sánchez, no me alcanzan las palabras para agradecerte haber sido la guía sin descanso de este camino, te agradezco por todas tus enseñanzas, por haber creído en mí incluso cuando las situaciones apuntarán a todo lo contrario, eres una de las personas que mas admiro y siempre me sentiré orgulloso de haber podido compartir contigo esta experiencia eres uan de las personas mas geniales y unas de las mas grandes científicas. Al Dr. Marc Valls por haber tenido toda esa tenacidad y ese positivismo para conmigo, tu apoyo, consejos y reglas me han llevado a culminar con éxito este viaje. Gracias por siempre haber tenido ese deseo altruista de guiarme por este camino, ayudarme no solo en las situaciones laborales sino también en las personales. IV Muchísimas gracias a ustedes dos por todos los consejos, gracias por haber impulsado incluso en contra de mismo, viviré siempre agradecido por haber sido esa fuente de inspiración y motivación. Gracias por no dejarme decaer y siempre haberme hecho sentir parte de una gran familia desde el primer día. To all my old and new lab mates, you were my family during these years I really will miss you all. Share with all of you this time was the most amazing adventure in my life. Thank you for all the support, for all advices and for all never-ending conversations. Thank you so much Alex, Marc, Marina, Crina, Eugenia, Jose, Liang, Pau, Brian, Anurag, you make my life really easy no matter how difficult where the times; your unconditional support, your eternal happiness sometimes too much, made this way unforgettable. I never will forget you. Al Gobierno Ecuatoriano y en especial a la SENESCYT por haberme otorgado la beca para poder realizar mis estudios de Doctorado. A todas las personas en el CRAG cuya ayuda y consejos fueron muy útiles durante esta etapa. INDEX vii TABLE OF CONTENTS ACKNOWLEDGMENTS ...................................................................................... I INDEX ............................................................................................................... v INTRODUCTION ............................................................................................... 1 Chapter1: Programmed Cell Death ............................................................... 3 Importance of programmed cell death in plant development and disease. 3 Chapter 2: Role of the Hypersensitive Response in plant immunity ............... 4 Plant immunity: an evolutionary fight against pathogens. .......................... 4 The Arabidopsis thaliana-Pseudomonas syringae interaction as genetic tool to understand Hypersensitive Response ............................................ 6 Hypersensitive response: a programmed cell death strategy to restricted pathogen attacks ...................................................................................... 7 Metacaspases as main regulators of HR ................................................... 9 OBJECTIVES .................................................................................................. 13 PUBLICATIONS .............................................................................................. 17 Informe del director de tesi del factor d’impacte dels articles publicats ........... 19 Dying two deaths — programmed cell death regulation in development and disease ....................................................................................................... 21 AtSERPIN1 is an inhibitor of the Metacaspase AtMC1-mediated cell death and autocatalytic processing in planta ........................................................ 31 Detection and Quantification of Protein Aggregates in Plants ...................... 57 DRAFT 1 ......................................................................................................... 67 AtHIR2, a new regulator of AtMC1-meiated cell death in plants....................... 69 Abstract .................................................................................................. 69 Introduction ............................................................................................ 69 viii Material and Methods ............................................................................. 74 Discussion .............................................................................................
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