Powerful, real-time, long-read sequencing in the palm of your hand Combining powerful real-time sequencing with complete portability, MinION devices deliver immediate access to gigabases of long-read data

MinION and MinION Mk1C allow you to sequence anything, anywhere — from the bench   sequencing technology is to the field — with real-time analysis providing immediate access to actionable results. The same DNA and RNA sequencing workflows are available across our products, advancing at an unprecedented pace, offering unrestricted read length, from short to ultra-long, and complete scalability promising a future where portable to suit your needs. sequencing will be routine in surveillance and many other fields. MinION™ Up to 50 Gb*

Jana Batovska, La Trobe University Flongle™ Up to 2.8 Gb*

GridION™ Up to 250 Gb (all 5 flow cells sequencing)*  MinION ~5 kb amplicon run basecalling

all done at 34.28 Gb, I’ll take that :) PromethION™ 24 and 48 Up to 7,000 Gb (P24) or 14,000 Gb (P48) (all 24 or 48 flow cells sequencing respectively)* Dr. John Tyson, University of British Columbia

1 10 100 1,000 10,000

Gigabases (log10 )*

* Theoretical max output when system is run for 72 hours (or 16 hours for Flongle) at 420 bases / second. Outputs may vary according to library type, run conditions, etc.

2 3 All the benefits of long-read, real-time nanopore sequencing in a portable, low-cost device

Long reads Portable Discover and phase hidden variation — from repetitive Sequence samples at source regions and structural variants — combine with VolTRAX™ for to novel, full-length transcript portable sample preparation isoforms

High yields Accessible As much as 50 Gb* data Starter Packs from just suitable for all applications $1,000 (MinION) and $4,900 — from whole genomes and (MinION Mk1C) — with no transcriptomes to high- capital investment or complex throughput targeted analyses IT infrastructure required

Real time Direct Immediate access to Study native DNA and RNA, actionable results — from not a copy — eliminate pathogen and antimicrobial amplification bias and detect resistance identification to base modifications fusion transcripts

* Theoretical max output when system is run for 72 hours at 420 bases / second. Outputs may vary according to library type, run conditions, etc.

4 5 How will you use your MinION?

From the bench to the field, MinION devices are being utilised throughout the world to deliver new insights and Cancer research actionable, real-time results Transcriptome for a range of applications. analysis Clinical research Environmental research

Human Whole genome genomics sequencing

Targeted Animal sequencing research

Basic genome RNA sequencing research Image courtesy of Dr. Sarah Stewart Johnson, Georgetown University.

Metagenomics Plant research

Microbiology

Epigenetics Microbiome

6 7 Portable DNA/RNA sequencing for anyone Choose your MinION Starter Pack

Small enough to fit in a pocket and powerful enough to deliver gigabases of data, the USB-powered MinION allows researchers to rapidly generate actionable biological Recommended insights across a wide range of application areas. Basic Enhanced

Sample added Sensor array with MinION device 1 1 Consumable flow cell to flow cell here multiple where the biology and for scaled-up Flow cells 1* 4 electronics come together sequencing for nanopore sequencing Sequencing kits 1 1

Wash kits 1 1

Community Support Included Included

Training included† - 1-day workshop

* Includes voucher for second free flow cell. † A wide range of training and support services are available, for more information visit $1,000 $3,300 store.nanoporetech.com/services.

Sensor chip (ASIC) with 512 active channels for data acquisition and control USB powered device; link to laptop or desktop computer to operate

Specification COMPATIBLE Weight Size 87 g (103 g with flow cell) W 105 mm | H 23 mm | D 33 mm

Buy now store.nanoporetech.com

8 9 Fully integrated, portable sequencing and analysis Choose your MinION Mk1C plan

MinION Mk1C combines the real-time, rapid, portable sequencing of MinION and Flongle with powerful integrated computing and a high-resolution touchscreen — offering a complete, go-anywhere solution for DNA and RNA sequencing and analysis. Basic Enhanced CapEx*

High-resolution Connected: LAN and Wi-Fi MinION Mk1C device 1 1 1 touchscreen display enabled — upload and share allowing complete your data, wherever you are Flow cells 6 12 - device control and easy visualisation of results Sequencing kits 1 2 - Integrated, powerful, real-time compute with Wash kits 1 1 - pre-installed basecalling and analysis software Software licence and device warranty† 12 months 12 months 12 months

Community Support Included Included Included

Data files are written to Training included‡ - 1-day workshop - an onboard, high- capacity SSD; data * Device purchase. † Extended warranties available. $4,900 $9,900 $9,300 can then be transferred ‡ A wide range of training and support services are available, to your own system visit store.nanoporetech.com/services for more information.

Use Flongle for smaller tests and analyses, or MinION Flow Cells for tens of gigabases of data

Specification COMPATIBLE Weight Size 420 g W 140 mm | H 30 mm | D 114 mm

Buy now store.nanoporetech.com

10 11 A complete and streamlined workflow for rapid access to actionable results

Bioinformatic capability needed:

Use cloud-based or local EPI2ME platform for real-time Library EPI2METM analysis workflows. preparation nanoporetech.com/analyse kits Small experiments or QC checks Explore your data and develop your bioinformatics skills EPI2ME Labs with interactive, best practice workflows and tutorials. nanoporetech.com/analyse

Community- Run open-source tools written and developed by the developed Nanopore Community. tools community.nanoporetech.com

Custom All the data, raw or basecalled, can be used in custom analysis analysis pipelines written by the user for specific VolTRAX pipelines applications.

More information nanoporetech.com/products

Prepare Sequence Analyse Applications include: • Streamlined library preps — in as little as • Sequence what you need, when and where you • Real-time results for time-critical applications • Rapid metagenomic species identification and 10 minutes, with multiplexing options need it such as pathogen identification antibiotic resistance profiling • Scale according to your needs — same chemistry • Read lengths determined by your sample and • User controlled run time — stop sequencing • Accurate high-coverage microbial genome and kits used for Flongle, MinION, GridION Mk1, experimental needs when sufficient data generated, wash and reuse assemblies (DNA and RNA) and PromethION • MinION devices sequence DNA and RNA directly flow cell • Enhanced large genome analysis (e.g. cancer • Automate library preparation using the portable, — meaning no amplification bias and retained • Portable data analysis using MinION Mk1C samples) through accurate mapping of structural USB-powered VolTRAX modification information or combine MinION with a laptop variation, repetitive regions, and phasing • Run smaller sequencing tests and experiments or • Output raw signal or basecalled .fastq files • Quantify and characterise RNA splice variants, cost-effectively check your sample quality using for use in custom analysis pipelines isoforms and fusion transcripts Flongle on MinION

12 13 Enhance your MinION sequencing workflow...... with data analysis in real time

Prepare Automated library preparation for nanopore sequencing. • Small, USB-powered device • Minimal hands-on time EPI2ME • Reproducible results Real time data analysis workflows nanopore.com/products accessed through the cloud or locally using MinION Mk1C†.

Example workflows: Sequence What’s In My Pot (WIMP) Species-level identification and Adapting MinION devices for smaller, rapid tests and quantification of microbes from analyses. Delivering as much as 2.8 Gb* data, Flongle metagenomic samples is suitable for: ARMA Builds on WIMP with full • Smaller samples (e.g. targeted regions and antibiotic resistance profiling smaller genomes) • Rapid sample ID or quality checking 16S Genus-level identification of bacteria and archaea in • Low-cost regular testing metagenomic samples nanopore.com/products Custom Reference Alignment Align genomes to any reference sequence Human SV Analyse Map and identify structural variation across the whole human genome Providing straightforward, best-practice data analysis workflows and interactive tutorials — from basic quality EPI2ME nanopore.com/analyse control to genome assembly. Labs • Minimal installation requirements Labs • Interactive tutorials for your data • Fully customisable nanopore.com/analyse

* Theoretical max output when system is run for 16 hours at 420 bases / second. Outputs may vary according to library type, run conditions, etc. † Coming soon.

14 15 Oxford Nanopore Technologies Phone: +44 (0)845 034 7900 Email: [email protected] Twitter: @nanopore www.nanoporetech.com

Oxford Nanopore Technologies, the Wheel icon, EPI2ME, Flongle, GridION, MinION, PromethION, and VolTRAX are registered trademarks of Oxford Nanopore Technologies in various countries.

© 2021 Oxford Nanopore Technologies.

Oxford Nanopore Technologies products are currently for research use only.

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