Welcome Address

We are honoured and pleased to welcome over 200 participants and guests from eighteen different Countries, at the International Symposium on the European outbreak of Xylella fastidiosa in olive, held in Apulia, Italy. The Scientific Committee with the collaboration on the invited speakers have built a scientific program which will provide an in depth and updated overview on this harmful pathogen, and illustrate the current situation in the outbreak area of Apulia. The researchers directly involved in the ongoing research activities on this new emerging threat will share, the knowledge and the data so far gathered, with the international scientific community and critically discuss the future steps and efforts to boost the ongoing research programs. As a complement to the core scientific program, the National and the Regional Plant Health Services have organized a specific session for the presentation and discussion of the phytosanitary and legislative measures to be adopted and implemented. The Symposium offers, with the field trip, a unique chance to visit the diseased area and have a look of the incidence and severity of the symptoms. We are delighted to invite a broad scientific community to this symposium. We very much hope that you will share our enthusiasm for this Symposium and we thank everybody who have decided to join us in this beautiful area.

The Organizing Committee

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Congress Board SCIENTIFIC COMMITTEE EDITORIAL GRAPHIC DESIGNER Giovanni Paolo MARTELLI Massimiliano MORELLI University of Bari Aldo Moro, Bari, Italy CNR Institute for Sustainable Plant Protection, Bari, Italy Rodrigo ALMEIDA WEBMASTERS University of California, Berkeley, USA Simona ABBÀ Domenico BOSCO CNR Institute for Sustainable Plant Protection, Torino, Italy University of Turin, Italy Pasquale SALDARELLI Jacob Dirk JANSE CNR Institute for Sustainable Plant Protection, Bari, Italy Dutch Inspection Service, Emmeloord, The Netherlands COLLABORATORS Alexander H. PURCELL Oriana POTERE University of California, Berkeley, USA Giovanna BOTTALICO ORGANIZING COMMITTEE Daniele CORNARA Donato BOSCIA Leonardo SUSCA CNR Institute for Sustainable Plant Protection, Bari, Italy Antonio CEGLIE Michele DIGIARO University of Bari Aldo Moro, Bari, Italy CIHEAM Mediterranean Agronomic Institute, Bari, Italy Michela CHIUMENTI Bruno Caio FARAGLIA Angelo DE STRADIS Ministry of Agricultural, Food and Forestry Policies, Italy CNR Institute for Sustainable Plant Protection, Bari, Italy Antonio GUARIO Toufic ELBEAINO Regional Phytosanitary Service, Apulia, Italy Franco VALENTINI Franco NIGRO Peter MOUBARAK University of Bari Aldo Moro, Bari, Italy CIHEAM Mediterranean Agronomic Institute, Bari, Italy Francesco PORCELLI Antonella SAPONARI University of Bari Aldo Moro, Bari, Italy Leonardo CALAPRICO Vito Nicola SAVINO Leonardo CATUCCI University of Bari Aldo Moro, Bari, Italy Maria FASANO CONGRESS SECRETARIAT Milena GUIDA Giuliana LOCONSOLE Vito MONTILON CNR Institute for Sustainable Plant Protection, Bari, Italy Donato PALMISANO Francesco PALMISANO Milena PETRUZZI CRSFA “Basile Caramia”, Locorotondo (Bari), Italy Maria PINTO Maria SAPONARI Paola POLLASTRO CNR Institute for Sustainable Plant Protection, Bari, Italy Maria Rosaria SILLETTI ADMINISTRATIVE SECRETARIAT Nicola TRISCIUZZI Grazia Valentina TRITTO Giuseppe ALTAMURA CNR Institute for Sustainable Plant Protection, Bari, Italy CRSFA “Basile Caramia”, Locorotondo (Bari), Italy Maria Grazia PIEPOLI CRSFA “Basile Caramia”, Locorotondo (Bari), Italy

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Acknowledgements

The INTERNATIONAL SYMPOSIUM ON THE EUROPEAN OUTBREAK OF XYLELLA FASTIDIOSA IN OLIVE has been organized by:

CNR INSTITUTE FOR SUSTAINABLE PLANT PROTECTION, BARI, ITALY DEPARTMENT OF SOIL, PLANT AND FOOD SCIENCE, UNIVERSITY OF BARI ALDO MORO, BARI, ITALY CRSFA RESEARCH CENTER “BASILE CARAMIA”, LOCOROTONDO (BARI), ITALY CIHEAM MEDITERRANEAN AGRONOMIC INSTITUTE, BARI, ITALY

The Organizing Committee would like to thank the following Italian institutions which have provided funds to develop this event:

MINISTERO PER LE POLITICHE AGRICOLE ALIMENTARI E FORESTALI REGIONE PUGLIA–AREA POLITICHE PER LO SVILUPPO RURALE-SERVIZIO AGRICOLTURA-OSSERVATORIO FITOSANITARIO REGIONALE

The Organizing Committee wishes to acknowledge the invited speaker for their invaluable scientific contributions and Prof. G. P. Martelli for precious help with abstract editing.

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The Organizing Committee wishes to thank the following organizations for their generous ongoing support:

GOLD SPONSORS

SILVER SPONSORS

BRONZE SPONSORS

The Organizing Committee is also grateful to the following organization for their kind participation:

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Key Information CONGRESS SECRETARIAT Dr. Maria Saponari, Dr. Giuliana Loconsole, Dr. Francesco Palmisano Mail to: [email protected].

During the congress, the Secretariat can be reached at +39.3403424074

CONGRESS VENUES Symposium sessions Costa Brada Luxury Resort, Litoranea Santa Maria di Leuca, Gallipoli (Lecce) Web: www.costabradaresort.it | Tel: +39.0833.202551 | Mail to: [email protected] Workshop sessions CRSFA Centro di Ricerca, Sperimentazione e Formazione in Agricoltura “Basile Caramia” Via Cisternino, 281, Locorotondo (Bari) Web: www.crsfa.it | Tel. +39.080.4313071 | Mail to: [email protected]

WEB SITE The Congress official website can be reached at: www.ivv.cnr.it/symposium-xylella-fastidiosa

ACCREDITATION PROCEDURES Symposium sessions are open to all registered participants. Your ID badge included in delegate‟s bags is required for admission to the Congress Centre and all Congress events. All enquiries should be directed to the registration helpdesk in the entrance hall. Registration Opening Hours: Monday 20th October 17:00−19:00 | Tuesday 21st October 8:30−18:30 | Wednsday 22nd October 8:30−12:30.

CERTIFICATE OF ATTENDANCE Certificate of Attendance will be included in the delegate‟s bag.

LANGUAGE English is the Congress official language. Simultaneous tanslation will be available.

INTERNET ACCESS General Wi-Fi access is available throughout the Congress Venue. Access password will be provided upon your request.

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POSTERS AND ORAL PRESENTATIONS Posters will be on display during symposium sessions in the dedicated area. Poster presenters are asked to mount their poster before the opening ceremony. Posters must be removed at the end of symposium sessions. Any posters remaining after this time will be removed by the organisers and cannot be reclaimed. Power Point Presentations, on USB flash drives, should be handed to the representative of the Congress Secretariat in advance but not later than 15 min prior to the start of the session.

CONGRESS SOCIAL DINNER Congress Dinner will take place on Tuesday 21st October at the Masseria Li Sauli, Gallipoli (www.lisauli.it). Participants will be asked to present tickets found in delegate‟s bag upon arrival at the event venue.

FIELD TRIP During field trip, scheduled on Wednesday 22nd October (14:00-17:00) participants will have opportunity to visit olive groves in the area were the main Xylella fastidiosa outbreak was first reported in October 2013. Please inform the Congress Secretariat if you are not willing to attend the field trip.

SHUTTLE BUS SERVICE Complimentary shuttle bus service from Brindisi/Bari airports to Gallipoli and viceversa will be available for participants attending the Symposium during its entire duration. Please at the airport look for the sign “Xylella”. On Thursday 23 October a shuttles bus will be available from Gallipoli to CRSFA Research Center (Locorotondo) for participants attending workshop training sessions. On Friday 24th October, and upon kind request on Saturday 25th October, shuttle bus service will accompany participants leaving workshop sessions to Brindisi/Bari airports.

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Touristic Tips CONGRESS LOCATIONS

SYMPOSIUM LOCATION:GALLIPOLI (LECCE)

Located on the southeastern coast of the „‟Italian heel‟‟, Gallipoli is celebrated for its crystal clear blue sea and the sandy beaches that make its territory one of the most cherished natural attractions in Salento. When viewed from the sea, Gallipoli‟s white skyline reveals itself in all of its oriental beauty, resembling a village of the Cyclades islands. The old town, perched on a limestone islet, is connected to the mainland via an arched bridge. The walls, ramparts and towers that once defended the city from foreign raiders, now provide shelter from the rough sea and confer the city a special charm that takes visitors back in time. Don‟t miss to visit the baroque cathedral dedicated to Saint Agatha and the Hellenistic fountain close to the bridge, rich in bas-reliefs depicting scenes from Greek mythology, and the Sanctuary of Our Lady of Canneto. The city‟s fascination adds to its beautiful nature, offering long beaches of very fine white sand that are mostly cherished by family vacationers and water sports fans.

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WORKSHOP LOCATION: LOCOROTONDO (BARI)

The village is located in Valle d‟Itria and you can enjoy the typical rural landscape from various sight of the historic centre with his small “trulli” locally called “casedde” and farms. A center of concentric streets, low houses with the typical pointed roof and whitewashed, originally built to fight against the sun and the pestilence, and then become the symbol of the country, the “cummerse“. Entering at the city centre by Napoli‟s door, you are immediately greeted by an efficient tourist information service, where you can find all the information materials, in various languages, to better organize your visit. Then you go in the nice built-up area made pleasant by the open spaces and by the white houses with main entrance and railings profiles stone. The food and wine is strongly linked to the local tradition: the white wine Locorotondo DOC and oil, and dishes such as orecchiette with broccoli and the mashed fava beans with wild chicory. In the midway between Cisterino and Alberobello, three are the stage unmissable (all certified with the Orange flag by Touring Club Italiano) a cultural and culinary excellence itinerary through the Itria valley.

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PROGRAMME SCHEDULE SYMPOSIUM SESSIONS

Monday, 20th October

17:00-19:00 REGISTRATION AND POSTER HANGING PROCEDURES

19:00-21:00 WELCOME RECEPTION AT COSTA BRADA RESORT, GALLIPOLI (LECCE)

Tuesday, 21st October

08:30-09:00 REGISTRATION AND POSTER HANGING PROCEDURES

09:00-09:30 WELCOME G. P. Martelli, Professor Emeritus, Department of Soil, Plant and Food Science, University

of Bari Aldo Moro, Italy

E. Lavarra, Representative of the Italian Minister of Agricultural, Food and Forestry Policies N. Vendola, President of the Apulia Regional Government F. Nardoni, Regional Assessor for Agriculture G. P. Accotto, Director of the CNR Institute for Sustainable Plant Protection A. Uricchio, Rector of the Aldo Moro University of Bari C. Lacirignola, General Secretary of the CIHEAM Mediterranean Agronomic Institute, Bari A.Palmisano, President of the CRSFA Research Center “B. Caramia”, Locorotondo (Bari)

XYELLA FASTIDIOSA: THE PATHOGEN, ITS EPIDEMIOLOGY AND MANAGEMENT SESSION I Chairman: Prof. G. P. Martelli

09:30-10:00 HISTORICAL PERSPECTIVES ON XYLELLA FASTIDIOSA AND THEIR RELEVANCE FOR THE FUTURE A. H. Purcell, Professor Emeritus, University of California, Berkeley, USA DISEASES INDUCED BY XYLELLA FASTIDIOSA SUBSP. PAUCA: ECOLOGY, EPIDEMIOLOGY AND 10:00-10:30 MANAGEMENT H. Della Coletta Filho, Centro de Citricultura Sylvio Moreira, IAC Cordeirópolis, São Paulo, Brazil 10:30-10:50 POTENTIAL VECTORS OF XYLELLA FASTIDIOSA IN EUROPE D. Bosco, R. Almeida, E. Czwienczek, G. Stancanelli, J. C. Gregoire, D. Caffier, G. Hollo, C. Bragard

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10:50-11:10 COFFEE BREAK

11:10-11:40 CONTROL STRATEGIES FOR XYLELLA FASTIDIOSA D. L. Hopkins, University of Florida, Central Florida Research and Education Center, USA 11:40-12:10 CELL DENSITY-DEPENDENT BEHAVIORS OF XYLELLA FASTIDIOSA: ACHIEVING DISEASE CONTROL VIA PATHOGEN CONFUSION S. E. Lindow, University of California, Berkeley, USA 12:10-12:30 SOME APPROACHES AIMING CITRUS VARIEGATED CHLOROSIS CONTROL IN BRAZIL A. A. de Souza, Centro de Citricultura Sylvio Moreira, IAC Cordeirópolis, São Paulo, Brazil

OLIVE QUICK DECLINE SYNDROME AND XYLELLA FASTIDIOSA EPIDEMICS IN SESSION II APULIA Chairman: Prof. A. H. Purcell

12:30-13:00 THE OLIVE QUICK DECLINE SYNDROME G. P. Martelli, Professor Emeritus, Dept. of Soil, Plant and Food Science, University of Bari Aldo Moro, Italy 13:00-13:20 IDENTIFICATION AND CHARACTERIZATION OF FUNGAL SPECIES ASSOCIATED WITH THE QUICK DECLINE SYNDROME OF OLIVE F. Nigro, I. Antelmi, A. Ippolito, Dept. of Soil, Plant and Food Science, University of Bari Aldo Moro, Italy 13:20-13:40 OCCURENCE OF XYLELLA FASTIDIOSA IN APULIA D. Boscia, CNR Institute for Sustainable Plant Protection, UOS Bari, Italy

13:40-15:00 LUNCH

15:00-15:15 SURVEY OF THE AUCHENORRHYNCHA IN THE SALENTO PENINSULA IN SEARCH OF PUTATIVE VECTORS OF XYLELLA FASTIDIOSA STRAIN CODIRO D. Cornara, G. Loconsole, D. Boscia, A. De Stradis, R. Y. Yokomi, D. Bosco, F. Porcelli, M. Saponari 15:15-15:30 OBSERVATIONS ON THE BIOLOGY AND ETHOLOGY OF APHROPHORIDAE: PHILAENUS SPUMARIUS (L.) IN THE SALENTO PENINSULA D. Cornara, F. Porcelli, Dept. of Soil, Plant and Food Science, University of Bari, Italy

DETECTION AND CHARACTERIZATION OF XYELLA FASTIDIOSA SESSION III Chairman: Prof. J. D. Janse

15:30-15:50 AN INNOVATIVE MONITORING MODEL FOR XYLELLA FASTIDIOSA IN APULIA REGION, ITALY A. M. D‟ Onghia, F. Santoro, T. Yaseen, K. Djelouah, A. Guario, A. Percoco, T. Caroppo, F.

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Valentini

15:50-16:10 MODELLING THE SPREAD OF XYLELLA FASTIDIOSA IN APULIA (SOUTHERN ITALY) S. M. White, J. M. Bullock, D. A. P. Hooftman, D. S. Chapman 16:10-16:30 INTERLABORATORY VALIDATION OF MOLECULAR AND SEROLOGICAL DIAGNOSIS OF XYLELLA FASTIDIOSA STRAIN CODIRO IN SUSCEPTIBLE HOSTS G. Loconsole, O. Potere, T. Elbeaino, D. Frasheri, S. Frisullo, F. Palmisano, D. Boscia, M. Saponari

16:30-16:50 COFFEE BREAK

16:50-17:10 ISOLATION, GENETICS AND PRELIMINARY DATA ON THE PATHOGENICITY OF THE XYLELLA FASTIDIOSA CODIRO STRAIN M. Saponari, G. Loconsole, R. Almeida, H. Della Coletta Filho, G. P. Martelli, D. Boscia 17:10-17:30 DRAFT GENOME SEQUENCE OF XYLELLA FASTIDIOSA STRAIN CODIRO A. Giampetruzzi, M. Chiumenti, M. Saponari, G. Donvito, A. Italiano, G. Loconsole, C. Cariddi, G. P. Martelli, P. Saldarelli 17:30-17:50 IDENTIFICATION AND CHARACTERIZATION OF XYLELLA FASTIDIOSA ISOLATED FROM COFFEE PLANTS IN FRANCE B. Legendre, S. Mississipi, V. Olivier, E. Morel, D. Crouzillat, K. Durand, P. Portier, F. Poliakoff, M. A. Jacques

17:50-18:30 POSTER VIEWING SESSION

20:00 SOCIAL DINNER AT MASSERIA LI SAULI, GALLIPOLI (LECCE)

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nd Wednesday, 22 October CURRENT ACTIONS FOR XYELLA FASTIDIOSA CONTROL SESSION IV Chairmen: B. C. Faraglia; G. Papa Pagliardini

08:30-08:45 OPENING REMARKS V. N. Savino, Dept. of Soil, Plant and Food Science, University of Bari Aldo Moro, Italy 08:45-09:15 PHYTOSANITARY REGULATION AGAINST XYLELLA FASTIDIOSA FROM A EUROPEAN UNION PERSPECTIVE G. Cardon, Directorate General for Health and Consumers, European Commission 09:15-09:45 MEASURES TAKEN BY THE ITALIAN PLANT PROTECTION SERVICE B. C. Faraglia, Ministry of Agricultural, Food and Forestry Policies, Italy A. Guario, Rural Development Policy Area, Regional Phytosanitary Service, Apulia, Italy 09:45-10:15 Risk assessment of Xylella fastidiosa for the EU G. Stancanelli, R. Almeida, D. Bosco, C. Bragard, D. Caffier, J. C. Gregoire, S. Parnell, G. Strona, O. Mosbach-Schulz, E. Czwienczek, G. Hollo

10:15-10:45 COFFEE BREAK

10:45-11:15 EPPO SUPPORTING THE EVOLUTION OF PHYTOSANITARY SYSTEMS IN EPPO MEMBER COUNTRIES F. Petter, F. Grousset & M. Suffert, EPPO European and Mediterranean Plant Protection Organization

11:15-12:15 OPEN DISCUSSION

12:15-12:30 CONCLUDING REMARKS M. Barba, Coordinator of the Advisory Scientific Committee for Xylella fastidiosa

12:30-13:30 LUNCH

14:00-17:30 FIELD TRIP

19:00-21.00 OLIVE PICKING TRIP: NIGHT HARVESTING OF OLIVES

21.00 BUFFET AT ANTICA MASSERIA DEL FANO, SALVE (LECCE)

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PROGRAMME SCHEDULE WORKSHOP SESSIONS

Thursday, 23th October 07:30- DEPARTURE FOR LOCOROTONDO (BARI) FOR THE TRAINING WORKSHOPS

09:30-10:00 COFFEE BREAK

TRAINING WORKSHOP A CURRENT TOOLS FOR THE DETECTION OF XYLELLA FASTIDIOSA IN HOST PLANTS

AND VECTORS

10:00-11:00 PRESENTATION OF THE COURSE AND OVERVIEW ON THE DIAGNOSTIC TOOLS M. Saponari, G. Loconsole, O. Potere, F. Palmisano, F. Valentini, H. D. Coletta Filho, D. Boscia 11:00-13:00 LABORATORY WORK: ELISA, DNA ISOLATION, PCR AND QPCR

13:00-14:30 LUNCH

14:30-18:00 LABORATORY WORK: ELISA, DNA ISOLATION, PCR AND QPCR (CONTINUATION)

TRAINING WORKSHOP B CHARACTERISTICS AND IDENTIFICATION OF XYLEM-SAP FEEDERS

PRESENTATION OF THE COURSE AND OVERVIEW ON TAXONOMY AND BIOLOGY OF INSECT 10:00-11:00 VECTORS OF XILELLA FASTIDIOSA A. H. Purcell, D. Bosco, F. Porcelli, D. Cornara, L. Picciau 11:00-13:00 LABORATORY WORK

13:00-14:30 LUNCH

14:30-18:00 LABORATORY WORK AND FIELD SESSION

19:30-20:30 VISIT TO A LOCAL WINERY

20:30 DINNER AT A LOCAL RESTAURANT

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Friday, 24th October

TRAINING WORKSHOPS A-B

08:30-16:30 LABORATORY WORK (CONTINUATION)

12:45-14:00 LUNCH

16:00-16.30 DISCUSSION AND CONCLUDING REMARKS

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