Catalog # Aliquot Size

P39-911-05 3 x 5 nmol P39-911-20 3 x 20 nmol P39-911-50 3 x 50 nmol

PTPN12 siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # P39-911

Lot # Z2085-23

Specificity Formulation PTPN12 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PTPN12 expression. shipped at room temperature.

Product Description Reconstitution Protocol PTPN12 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PTPN12 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_002835. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot PTP-PEST, PTPG1, tcag7.1075 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or PTPN1 (PTP1B), Active P28-20G below -20oC. After resuspension, siRNA stock solutions ≥2 PTPN11 (SHP2), Active P38-21G µM can undergo up to 50 freeze-thaw cycles without PTPN12 (PTP-PEST), Active P39-21G significant degradation. For long-term storage, it is PTPN13 (FAP-1), Active P40-21G recommended that the siRNA is stored at -70oC. For most PTPN2 (TC-PTP), Active P29-20G favorable performance, avoid repeated handling and PTPN6 (SHP1), Active P33-20G multiple freeze/thaw cycles. PTPN7 (LC-PTP), Active P34-20G Anti-PTPN11 (SHP2) P38-63CR Scientific Background Anti-PTPN11 (SHP2) P38-63BR Protein tyrosine phosphatase-PEST (PTPN12), a ubiquitously expressed cytoplasmic tyrosine phosphatase, is thought to play an important role in cell adhesion and motility, cell migration, and signal transduction for PTPN12 siRNA Set I siRNA duplexes targeted against three exon regions antigen receptors in B and T lymphocytes (1). Signal transduction via tyrosine phosphorylation, normally fine- Catalog # P39-911 tuned by the concerted action of both protein tyrosine Lot # Z2085-23 kinases and protein tyrosine phosphatases (PTPs), is a key Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol mechanism in tumorigenesis. Studies suggest potential Format Lyophilized powder role for PTP-PEST in regulation of p130 (cas) in mitogen- Stability 1yr at -70oC from date of shipment and cell adhesion-induced signaling events (2). Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized References and resuspended siRNAs are stored at 1. Angers-Loustau, A. et al: Protein tyrosine or below -20oC. After resuspension, phosphatase-PEST regulates focal adhesion siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles disassembly, migration, and cytokinesis in fibroblasts. without significant degradation. For J. Cell Biol. 1999; 144: 1019-31. long-term storage, it is recommended 2. Garton, A.J. et al: Identification of p130 (cas) as a that the siRNA is stored at -70oC. For most favorable performance, avoid substrate for the cytosolic protein tyrosine repeated handling and multiple phosphatase PTP-PEST. Mol. Cell Biol. 1996; freeze/thaw cycles. 16(11):6408-18.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.