UGT8 Antibody Cat. No.: 42-832

UGT8 Antibody

Specifications

HOST SPECIES: Goat

HOMOLOGY: Expected Species Reactivity based on : Human, Rat, Dog, Cow

IMMUNOGEN: The immunogen for this antibody is: C-NGKYKRNGHIK

TESTED APPLICATIONS: WB

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:In transfected MCF7 transiently expressing Human UGT8 a band of approx. 65kDa is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5kDa according to NP_003351.2. Recommended concentration, 1-3ug/ml. Preliminary experiments gave an approx 50kDa band in Human -Brain lysates and 48KDa in Rat Brain APPLICATIONS: lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.5kDa according to NP_003351.2. The observed band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

SPECIFICITY: Reported variants represent identical protein: NP_001121646.1, NP_003351.2.

POSITIVE CONTROL: 1) Cat. No. 1303 - Human Brain Tissue Lysate

2) Cat. No. 1461 - Rat Heart Tissue Lysate

Properties

September 30, 2021 1 https://www.prosci-inc.com/ugt8-antibody-42-832.html Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

CONCENTRATION: 500 ug/mL

STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing.

Additional Info

OFFICIAL SYMBOL: UGT8

UGT8, UDP 8, CGT, UGT4, 2-hydroxyacylsphingosine 1-beta- , UDP-galactose galactosyltransferase, UDP-galactose- ALTERNATE NAMES: ceramide galactosyltransferase, ceramide UDP-galactosyltransferase, cerebroside synthase, uridine diphosphate glycosyltransferase 8

ACCESSION NO.: NP_003351.2

PROTEIN GI NO.: 40254471

GENE ID: 7368

ANTIBODIES FOR RESEARCH USE ONLY.

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September 30, 2021 2 https://www.prosci-inc.com/ugt8-antibody-42-832.html