UGT8 Antibody Cat. No.: 42-832
UGT8 Antibody
Specifications
HOST SPECIES: Goat
HOMOLOGY: Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Cow
IMMUNOGEN: The immunogen for this antibody is: C-NGKYKRNGHIK
TESTED APPLICATIONS: WB
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:In transfected MCF7 transiently expressing Human UGT8 a band of approx. 65kDa is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5kDa according to NP_003351.2. Recommended concentration, 1-3ug/ml. Preliminary experiments gave an approx 50kDa band in Human -Brain lysates and 48KDa in Rat Brain APPLICATIONS: lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.5kDa according to NP_003351.2. The observed band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
SPECIFICITY: Reported variants represent identical protein: NP_001121646.1, NP_003351.2.
POSITIVE CONTROL: 1) Cat. No. 1303 - Human Brain Tissue Lysate
2) Cat. No. 1461 - Rat Heart Tissue Lysate
Properties
September 30, 2021 1 https://www.prosci-inc.com/ugt8-antibody-42-832.html Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide.
CLONALITY: Polyclonal
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION: 500 ug/mL
STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing.
Additional Info
OFFICIAL SYMBOL: UGT8
UGT8, UDP glycosyltransferase 8, CGT, UGT4, 2-hydroxyacylsphingosine 1-beta- galactosyltransferase, UDP-galactose ceramide galactosyltransferase, UDP-galactose- ALTERNATE NAMES: ceramide galactosyltransferase, ceramide UDP-galactosyltransferase, cerebroside synthase, uridine diphosphate glycosyltransferase 8
ACCESSION NO.: NP_003351.2
PROTEIN GI NO.: 40254471
GENE ID: 7368
ANTIBODIES FOR RESEARCH USE ONLY.
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September 30, 2021 2 https://www.prosci-inc.com/ugt8-antibody-42-832.html