International Journal of (2001) 25, Suppl 5, S63–S67 ß 2001 Nature Publishing Group All rights reserved 0307–0565/01 $15.00 www.nature.com/ijo PAPER The as a conduit for diverse signals relevant to energy

RD Cone1*, MA Cowley1, AA Butler1, W Fan1, DL Marks1 and MJ Low1

1Vollum Institute, Oregon Health and Science University, Portland, Oregon, USA

Arcuate nucleus are known to be responsive to a wide array of and nutrients, including , , gonadal steroids and glucose. In addition to potential transport mechanisms, peripheral substances may access these neurons via arcuate cell bodies in and projections to the , a region considered to be a circumventricular organ. The arcuate is a potent site of leptin action, probably mediating a component of leptin’s effects via arcuate Y=agouti- related (NPY=AgRP) and pro-opiomelanocortin (POMC) neurons, and implicating this structure in the long-term control of energy stores. However, , the endogenous ligand of the growth secretagogue receptor, may also stimulate feeding and weight gain, in part through action on receptors in arcuate NPY neurons. Since ghrelin is secreted by the stomach upon content depletion, with a half-life of no more than an hour, the arcuate nucleus may also be important in sensing and responding to acute changes in nutrients. We have developed a system for recording from arcuate POMC neurons using a mouse containing a transgene in which the POMC promoter is driving expression of the green fluorescent protein (GFP). In these mice, 99% of the b-endorphin positive neurons express GFP, making whole cell patch clamp recordings from the sparsely distributed POMC neurons facile. All of the POMC neurons appear to be activated by leptin, via two different mechanisms, while approximately 30 – 50% of the neurons appear to be inhibited by a gamma-melanocyte stimulating hormone (MSH) specific agonist. The latter result suggests that the melanocortin-3 receptor (MC3-R) may act as an autoinhibitory receptor on some POMC neurons. This hypothalamic slice preparation also confirms the responsiveness of arcuate POMC neurons to a wide variety of nutrients and hormones. Thus the arcuate melanocortin system is best described as a conduit of many diverse signals involved in energy homeostasis, with leptin acting tonically to regulate the responsiveness of the circuit to a wide variety of hormones and nutrients. International Journal of Obesity (2001) 25, Suppl 5, S63 – S67

Keywords: leptin, POMC; ; AgRP; MC3-R; MSH; regulation

With the discovery of the adipostatic hormone leptin,1 orexigenic hypothalamic NPY was responsible for a compo- attention was quickly turned to defining neural circuits nent of the obesity syndrome in the leptin-deficient mouse.5 responsible for mediating the leptin signal in the . A This hypothesis was confirmed when it was demonstrated number of studies identified the sites of expression of the that crossing the Lepob=Lepob into the NPY 7 = 7 back- leptin receptor in the (CNS),2,3 sites ground reduced the obesity of the Lepob=Lepob by approxi- of fos activation by both peripheral and central leptin mately 50%.6 Moreover, studies on the melanocortin-4 administration,4 as well as sites of activation of suppressors receptor7 and the mechanism behind the obesity in the of cytokine signaling-3 (SOCS-3) by leptin. Even prior to agouti (Ay) mouse8,9 also identified the central pro-opiome- studies on the effect of the leptin gene product on neuropep- lanocortin (POMC) neurons as a critical component of the tide Y (NPY), data suggested that aberrant regulation of the energy homeostasis circuit. Since lesions in central POMC signaling cause obesity in both mice and humans, this circuit clearly plays an anorexigenic role. Furthermore, the neuro- peptide Y=agouti related-peptide (NPY=AGRP) and POMC genes are inversely regulated by leptin and metabolic state. These findings led many to assume that the primary role of *Correspondence: RD Cone, Vollum Institute L474, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland the melanocortin circuit was to transmit the anorexigenic 97201-3098, USA. E-mail: [email protected] signal arising from elevated levels of serum leptin, and this The accurate nucleus RD Cone et al S64 has been implied many times in the literature from diagrams double muant animals were significantly more obese of this circuit such as that shown in Figure 1. (Figure 2a), and significantly more hyperinsulinemic Shortly after the identification of a role for the central (Figure 2b) than Lepob=Lepob, implying roles for the melano- melanocortin system in energy homeostasis, however, we cortin circuitry that are independent and additive to the role performed a very simple experiment that argued strongly for in transmitting the leptin signal. Not surprisingly, the find- a broader role for the arcuate melanocortin system.10 ing was largely ignored! In retrospect, the result makes sense Lepob=Lepob and Ay mice were crossed to produce double in light of both earlier studies as well as new results demon- mutant animals, and then adrenalectomized and provided strating the arcuate nucleus and POMC neurons are sensitive corticosterone in their drinking water to control for the to a remarkably wide array of hormones and nutrients. For peripheral and central anabolic effects of the very high example, POMC neurons project to gonadotropin-releasing glucocorticoids seen in the Lepob=Lepob. Surprisingly, the hormone (GnRH) neurons, and the POMC gene is regulated by gonadal steroids.11 The arcuate is a dense site of insulin receptor expression, and a site of glucose-responsive neurons as well. More recently, arcuate NPY neurons have been demonstrated to express secretagogue receptor (GHS-R) or ghrelin receptor,12 the receptor for the stimulatory factor, ghrelin, produced by the sto- mach.13,14 Additionally, the arcuate is a unique hypothala- mic site expressing fatty acid synthase, and has been proposed as a site sensitive to intermediates of fatty acid metabolism.15 Given the importance of the central melano- cortin system in mediating the leptin signal, and the many hormones and nutrients the arcuate appears capable of sensing, it is perhaps not surprising that the Lepob=Lepob mouse is still capable of sensing and regulating adipose mass, even in the complete absence of the primary adipo- static factor, leptin (Figure 3). Below, I will describe new results on the melanocortin system that help explain this curious finding. As mentioned above, a variety of defects in melanocortin signaling will cause obesity in both mice and humans. However, these findings do not tell us the specific physio- logical roles for the melanocortin system in the normal animal. It is incorrect to assume that melanocortin-4 recep- tor (MC4-R) deficient animals, for example, have a gross defect in matching energy intake with energy expenditure. In fact, the animals actually respond quite normally to several challenges to homeostasis, such as food restriction or exposure to 4C. In both these cases, we have demon- strated that MC4-R 7 = 7 mice respond very comparably to wild-type mice. For example, in the food restriction para- digm the MC4-R 7 = 7 and wild-type mice lose weight at a similar rate, and after being given food ad libitum both animals return to their previous weight with similar kinetics. Thus, the defect in regulation leading to obesity in this strain is subtle. Recent experiments suggest an important role for MC4-R signaling in sensing and=or responding to acute changes in nutrient intake.16 When normal C57BL=6J mice are switched from normal low-fat chow (  11% energy as fat) to moderate-fat chow (25% fat), they eat slightly less and show increased thermogenesis by indirect calorimetry (com- monly called diet-induced thermogenesis, DIT). Conse- quently, the growth rate remains fairly constant for at least a week after the switch. In contrast, the MC4-R 7 = 7 mouse Figure 1 An early model of the role of arcuate NPY=AGRP and POMC undergoes a dramatic increase in the rate of weight gain circuits in the control of energy homeostasis. when switched to the moderate-fat chow. These animals do

International Journal of Obesity The accurate nucleus RD Cone et al S65

Figure 2 Ay and Lepob=Lepob lesions have an additive effect on weight gain (A) and fasting serum insulin levels (B), following adrenalectomy and normalization of glucocorticoids with corticosterone in the drinking water. Data is reprinted with permission from Science.10 The genetic strains indicated are all in the C57BL=6J background.

not decrease their food intake, in fact it actually increases, and furthermore the animal appears completely defective in DIT. Remarkably, the Lepob=Lepob mouse exhibited a robust DIT response in this diet paradigm. The results suggest a unique role for the melanocortin circuitry in sensing and=or responding to acute changes in nutrient intake that are likely to be important in time frames during which changes in leptin levels do not occur. It is thus possible to imagine that mice or humans with defective melanocortin signaling may thus become obese due, in part, to dysregulation of the coordination of intake with expenditure, independent of possible disruption of the normal leptin signal, thus Figure 3 Short-term homeostatic mechanisms exist in the Lepob=Lepob explaining the apparent additive effects of the Lepob=Lepob mouse. Declining weight curves represent periods of food restriction to and Ay lesions on weight gain.10 40% of normal intake levels for each strain, while periods of weight gain or stabilization occur during feeding ad libitum. To begin to test the ability of the arcuate nucleus to respond to a variety of hormones and nutrients, we sought to develop an electrophysiological model system for char- acterizing the activity of the POMC neurons. Since both POMC and NPY=AGRP neurons comprise only a fraction of total arcuate neurons, a transgenic line (POMC-EGFP) was created using the hypothalamic POMC promoter driving expression of enhanced GFP. Co-localization studies using an antibody against the POMC peptide b-endorphin demon- strated that greater than 99% of arcuate POMC expressed detectable GFP (Figure 4). Whole cell patch clamp recordings were then used to characterize the responsiveness of these neurons to leptin.17 All POMC neurons appear to exhibit spontaneous action potentials, and leptin depolarized and increased the firing rate of nearly every POMC tested (Figure 5). Leptin was found to activate these neurons Figure 4 Visualization of arcuate POMC neurons in the POMC-EGFP directly, by activating a non-specific cation channel on the transgenic mouse. Fluorescent microscopy indicates the distribution of POMC neuron, and indirectly by inhibiting the release of EGFP-positive POMC cell bodies in portion of the arcuate nucleus of a mouse expressing a POMC-EGFP transgene. Reprinted with permission gamma-aminobutyric acid (GABA) from NPY terminals from Nature.17 synapsing onto POMC neurons (Figure 6). This preparation

International Journal of Obesity The accurate nucleus RD Cone et al S66 circumventricular organ, existing functionally outside the blood – brain barrier. For example, the large serum polypep- tide growth hormone is known to access arcuate growth hormone-releasing hormone (GHRH) neurons, where it acts as an autoinhibitory signal for GHRH release. While the mechanisms involved are not clear, not only hypophy- siotropic neurons, but arcuate POMC and NPY neurons have also been demonstrated to send fibers into the median eminence. From the data discussed here we would argue that the role of the arcuate melanocortin system in energy homeostasis may be quite a bit more complex than originally proposed (Figure 1). This system may act to sense and respond to a wide variety of hormone and nutrient signals relevant to both short- and long-term aspects of the regula- Figure 5 Leptin depolarizes and increases the frequency of sponta- tion of energy homeostasis, integrating these diverse signals neous action potentials in arcuate POMC neurons. Whole cell patch clamp recording of a POMC-EGFP neuron demonstrates the effect of for transmission to motor centers such as the paraventricular bath administration of 100 nM leptin (arrow). Reprinted with permission nucleus, lateral hypothalamic area, and brainstem (Figure 7). from Nature.17

Figure 6 NPY=AGRP and POMC neurons act as an interconnected circuit. Electrophysiological studies indicate that leptin, melanocortins, ghrelin and other hormones and nutrients act on both NPY=AGRP and POMC neurons to control the activity of the POMC neuron. The reciprocal effects on NPY neurons has not yet been examined in this preparation. Abbreviations: Lep-R, leptin receptor; insulin-R, insulin receptor; MC3-R, melanocortin-3 receptor; Y1-R, Y2-R, neuropeptide Y1 or Y2 receptors; MOP-R, m- receptor. Figure 7 The arcuate nucleus is a circumventricular organ, with access to the and portal vasculature, which is responsive to a wide variety of hormones and nutrients. has allowed us to demonstrate that the melanocortin-3 receptor (MC3-R) is an inhibitory autoreceptor for POMC neurons, and the ghrelin receptor on NPY neurons also affects the POMC neuron by stimulating GABA release References onto the POMC cells (unpublished data). This preparation 1 Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman has also been used to demonstrate that arcuate POMC JM. Positional cloning of the mouse obese gene and its human neurons are also responsive to glucose, insulin, and inter- homologue. Nature 1994; 372: 425 – 432. leukin-1b (IL-1b; unpublished data). 2 Huang XF, Koutcherov I, Lin S, Wang HQ, Storlien L. Localization of leptin receptor mRNA expression in mouse brain. Neuroreport Situated between the third ventricle and the median 1996; 7: 2635 – 2638. eminence, in which the portal vascular system functions to 3 Hakansson ML, Hulting AL, Meister B. Expression of leptin transport neuroendocrine releasing factors to the anterior receptor mRNA in the hypothalamic arcuate nucleus — relation- ship with NPY neurones. Neuroreport 1996; 7: 3087 – 3092. pituitary, the arcuate nucleus is uniquely positioned to 4 Elmquist JK, Ahima RS, Maratos Flier E, Flier JS, Saper CB. Leptin sample factors in both blood and cerebrospinal fluid. The activates neurons in ventrobasal and brainstem. arcuate nucleus has long been known to function as a 1997; 138: 839 – 842.

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International Journal of Obesity