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Datasheet: AHP1176 Product Details Datasheet: AHP1176 Description: RABBIT ANTI HUMAN CTRP5 Specificity: CTRP5 Other names: C1q AND TNF RELATED PROTEIN 5 Format: Purified Product Type: Polyclonal Antibody Isotype: Polyclonal IgG Quantity: 0.1 mg Product Details Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.bio-rad-antibodies.com/protocols. Yes No Not Determined Suggested Dilution Flow Cytometry Immunohistology - Frozen Immunohistology - Paraffin ELISA Immunoprecipitation Western Blotting 1 - 5ug/ml Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Target Species Human Species Cross Based on sequence similarity, is expected to react with:Mouse, Rat Reactivity N.B. Antibody reactivity and working conditions may vary between species. Product Form Purified IgG - liquid Antiserum Preparation Antisera to human CTRP5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. Buffer Solution Phosphate buffered saline Preservative 0.02% Sodium Azide Stabilisers Approx. Protein IgG concentration 1.0mg/ml Concentrations Immunogen A 15 amino acid peptide from near the centre of human CTRP5. Page 1 of 3 External Database Links UniProt: Q9BXJ0 Related reagents Entrez Gene: 114902 C1QTNF5 Related reagents Synonyms CTRP5 Specificity Rabbit anti Human CTRP5 antibody recognizes C1q and TNF related protein 5 (CTRP5), also known as C1QTNF5. CTRP5 encodes a novel short chain collagen which is widely expressed with highest expression in the retinal pigment epithelium (RPE) and ciliary epithelium (CE). It is a secretory protein, which associates with membrane type frizzled related protein (MFRP), and may aid the adhesion of basal RPE to Bruch's membrane. Mutation to CTRP5 causes it to be retained in the endoplasmic reticulum, and can lead to late-onset retinal degeneration (LORD) and long anterior lens zonules (Subrayan et al.2005). CTRPs are often highly modified post-translationally, so whereas the predicted molecular weight for CTRP5 is ~25 kDa, Rabbit anti Human CTRP5 antibody detects a single band at ~40 kDa in western blotting. http://www.omim.org/entry/605670 https://www.ncbi.nlm.nih.gov/pubmed/16376663 Western Blotting AHP1176 detects a band of approximately 39kDa in caco-2 cell lysate. Further Reading 1. Shu, X. et al. (2006) Disease mechanisms in late-onset retinal macular degeneration associated with mutation in C1QTNF5. Hum. Mol. Genet. 15: 1680-1689. 2. Ayyagari, R. et al. (2005) Late-onset macular degeneration and long anterior lens zonules result from a CTRP5 gene mutation. Invest. Ophthalmol. Vis. Sci. 46: 3363-3371. Storage Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life 18 months from date of despatch. Health And Safety Material Safety Datasheet documentation available at: Information Material Safety Datasheet Documentation #10040 available at: https://www.bio-rad-antibodies.com/uploads/MSDS/10040.pdf Regulatory For research purposes only Related Products Recommended Secondary Antibodies Sheep Anti Rabbit IgG (STAR34...) FITC Sheep Anti Rabbit IgG (STAR35...) RPE Goat Anti Rabbit IgG (H/L) (STAR124...) HRP Goat Anti Rabbit IgG (Fc) (STAR121...) Biotin, FITC, HRP Sheep Anti Rabbit IgG (2AB02...) Biotin Page 2 of 3 Sheep Anti Rabbit IgG (STAR36...) DyLight®488, DyLight®549, DyLight®649, DyLight®680, DyLight®800 Recommended Useful Reagents TidyBlot™ WESTERN BLOT DETECTION REAGENT:HRP (STAR209P) North & South Tel: +1 800 265 7376 Worldwide Tel: +44 (0)1865 852 700 Europe Tel: +49 (0) 89 8090 95 21 America Fax: +1 919 878 3751 Fax: +44 (0)1865 852 739 Fax: +49 (0) 89 8090 95 50 Email: [email protected] Email: [email protected] Email: [email protected] 'M294354:160929' Printed on 13 Jan 2018 © 2018 Bio-Rad Laboratories Inc | Legal | Imprint Page 3 of 3.
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