Assaad A. Eid Summary

I am currently leading one of the most funded laboratories in Lebanon and the middle east, with more than 7 million US dollars in funding. This laboratory is involved in internationally cutting-edge basic, translational and clinical science research in the field of diabetes and diabetic complications. Our research activities are part of advancing efforts to find new treatments that will, hopefully, lead us closer to identifying potential cure for the most recognized debilitating complications of diabetes. These complications include nephropathy, neuropathy, cardiomyopathy, and, the most recent complication recognized, diabetes-induced cancer. All of these systems are being studied from different standpoints: metabolic alterations, genetic predisposition and epigenetic influence. Besides, I co-founded in 2017 the American University of Beirut (AUB) diabetes to integrate scientific and clinical knowledge to enhance our understanding of the underlying pathologies of diabetes from both the translational (bench to bedside) and clinical perspectives. Likewise, and since 2015, I was appointed as the Faculty of Medicine Masters’ Programs director. In this capacity I oversee 9 basic and clinical masters programs (Pharmacology and Toxicology, Physiology, Human Morphology, Neuroscience, Immunology and Title and affiliation Microbiology, Biochemistry and Molecular Genetics, Orthodontics, Scholars in HeAlth Research Program (SHARP), and Plastic Surgery) with more than a Associate Professor hundred students enrolled. Furthermore, and since I joined AUB, I have trained Faculty of Medicine and Medical Center more than 50 masters and medical students, more than 15 PhD students and 9 Department of Anatomy Cell Biology postdocs. In recognition to my contribution to research and training, in 2016, I was and Physiological Sciences appointed as Visiting Professor at Paris Descartes University, Paris, France and American University of Beirut to join the International Diabetic neuropathy Consortium as an international Beirut, Lebanon. collaborator. Likew. ise, in addition to several national and international scientific awards, I was elected as a member in the Inter-academy Medical Panel - Young physician Leaders, young affiliate member of The World Academy of Sciences for the Mailing Address developing world and won the first prize in the startup competitions of the Beirut International Healthcare Industry Forum. P.O. Box 11-0236 In addition to my academic and industrial research activity, I am involved in several teaching activities internationally and at the level of AUB. In recognition to Riad El Solh 1107 2020 my International teaching activities, in 2016, I was appointed as a Visiting Beirut, Lebanon Professor at Paris Descartes University, Paris France. Likewise, at the level of Cell Phone: 961-71-873573 AUB, Beside my role as an educator who believes that teaching is a mutual Office Phone: 961-1-350000 Ext: 4781 adventure taken by the professor and the students, I am responsible for Lab Phone: 961-1-350000 Ext: 4771 coordinating medical teaching and overseeing graduate teaching. Fax: 961-1-744474 E-Mail: [email protected] Professional profile • Educator who believes that education is the process of acquiring the science and the art of teaching and learning Citizenship • Highly Knowledgeable in translational research, and the design and management of clinical trials. France and Lebanon • Knowledge and understanding of the scientific peer-review process, the mechanics of grant making and the culture of research academic medical centers • Familiarity with the culture of corporate science and the drivers for decision-making within that culture • Proven experience in identifying and cultivating key partner relationships • Goal-oriented, creative professional who has a proven record of strategic thinking and a strong desire to make a difference • Excellent interpersonal skills, including the ability to interact with scientists and non-scientists at a variety of levels in a collaborative, effective manner; a record of success in working with a team to achieve common goals • Proven track record in bringing academic and corporate science together to achieve diagnostic and therapeutic progress • Entrepreneurial spirit and ability to thrive in small, fast-growth organization • Service-focused, innovative and Complex problem solver

Collaborative Achievements

• Coordinator of the biomedical and research exchange program (University wide exchange program) and the “Cotutelle” agreement of higher education between the American University of Beirut and Paris Descartes University – Sorbonne Paris Citée, Paris, France. This is the first of its kind program between a French and American institutions that will allow Faculty members and Lebanese Students to travel to France and participate in the cultural, social, educational and research programs at the Paris Descartes University – Sorbonne Paris Citée and vice versa, where the French Faculty members and students can come to AUB and participate in their counterpart programs.

• Coordinator of the basic and translational research exchange program between AUB and Central Clinical School at Monash University, Melbourne Australia. It’s the first of its kind funded project by the Australian Department of Foreign Affairs and Trade to encourage medical, cultural and educational exchange programs between Lebanese institutions and Australian institutions.

• Coordinator of the medical and research exchange program between AUB and the University of Michigan at Ann Arbor, USA

Education

2018-2020 Executive Master of Business Administration Institution: Olayan School of Business, American University of Beirut, Beirut, Lebanon. 2015 Habilitation (Habilitation to Direct Research; HDR) Institution: Paris Descartes University, Faculty of Applied and Biomedical Sciences, Paris, France. 2002-2006 Doctorate Degree Major: Endocrinology and Metabolism Institution: Claude Bernard University, Faculty of Medicine Laennec, Lyon, France. 2000-2002 Master’s Degree in Biomedical Engineering (MSc) Institution: Claude Bernard University, Faculty of Medicine Laennec, Lyon, France.

Professional Experience

2019-Present Advisor to the Lebanese Prime Minister for matters related to Higher Education and Research. 2017-Present Founding Co-Director of the AUB Diabetes Institution: Faculty of Medicine, American University of Beirut, Beirut, Lebanon. 2016-Present Visiting Professor Institution: Faculty of Applied and Biomedical Sciences, Paris Descartes University, Paris, France.

2015-Present Associate Professor Institution: Faculty of Medicine, American University of Beirut, Beirut, Lebanon. 2015-Present Director of the Faculty of Medicine Masters’ Programs Institution: Faculty of Medicine, American University of Beirut, Beirut, Lebanon. 2010-2015 Assistant Professor Institution: Faculty of Medicine, American University of Beirut, Beirut, Lebanon.

2006-2010 Institution: Department of Medicine, Division of Nephrology, University of Texas Health Science Center at San Antonio, Texas, USA.

Honors and Awards

2018 Horizon 2020 Best Researcher Institution: Lebanese Association for the Advancement of Sciences 2017 Beirut International Healthcare Industry Forum - First Prize in the startup competition Institution: Lebanese ministry of Health, Lebanon. 2016 French/Lebanese Scientific Excellence Award “Prix d’Excellence Scientifique Franco-Libanais” Institution: Institut Français, French Embassy, Beirut, Lebanon. 2013 Elected in the Interacademy Medical Panel - Young physician Leaders Institution: Interacademy Medical Panel 2013 Representing Lebanese Youth in the World Health Summit Institution: World Health Summit 2013 Scientific Research Award – First Prize Institution: Mohamad Chaeib Foundation 2012 Elected young affiliate of The World Academy of Sciences for the Developing world Institution: The World Academy of Sciences for the Developing world (TWAS) 2009 Best Fellow Institution: National Kidney Foundation 2009 12th Annual Research Day Winner: Postdoctoral fellow category Institution: University of Texas Health Science center at San Antonio.

Professional Membership – National and International

2018-Present Member of the American Educational Research Association 2018-Present Member of the European Association for International Education 2016-Present Member, ISANH Middle East Antioxidants Committee 2016-Present Founding President, Lebanese Association for Diabetes Research (LADR) 2012-Present Member, American Heart Association 2012-Present Young affiliate, The World Academy of Sciences (TWAS) 2012-Present Elected in the Interacademy Medical Panel - Young physician Leader 2011-Present Member, Lebanese Association for the Advancement of Sciences Annual Meetings Organizing Committee 2011-Present Member, Lebanese Association for the Advancement of Sciences (LAAS) 2010-present Member, Lebanese Association for the Advancement of Sciences (LAAS) 2008-present Member, American Society of Nephrology

Academic Membership Memberships in Departmental Committees (Department of Anatomy, Cell biology and Physiology

2017-Present Member of The Course Taskforce. 2017-Present Member of The Graduate Advisory Committee. 2017-2019 Chair of The Graduate Advisory Committee. 2011-2019 Director/Advisor of the Graduate Master Programs (3 programs) of Physiology, Human Morphology and Neurosciences.

Memberships in Faculty Committees (Faculty of Medicine)

2017-Present Member of the task force appointed by the Dean for revising the structure of the medical curriculum 2015-Present Director of the Faculty of Medicine Graduate Masters Programs 2014-Present Member of the second year Medical Teaching Committee 2013-Present Member of PRIME “Program for Research and Innovation in Medical Education” 2013-Present Member of the Graduate Committee 2012-Present Member of the first year Medical Teaching Committee 2013-2019 Member of the Faculty of Medicine Research Committee 2012-2013 Member for the Purchasing task force 2012-2013 Member of the task force to review and evaluate the state of graduate studies 2011-2014 Co-Director of the Renal and Cardiovascular Research group

Memberships in University Committee

2019-Present Member of the University Committee on Students Affair of the Senate 2018-Present Member of the Intellectual Property Advisory Committee 2017-Present Member of the Research Innovation Committee 2017-Present Member of the Healthcare Technology Innovation committee 2017-Present Member of the Innovation Strategy committee (AUB-iPark) 2011-Present Member of the Annual AUB Biomedical Research Day Organizing committee

Peer Reviewed Publications (*Corresponding Author)

1. Abou Daher A, Francis M, Azzam P, Ahmad A, Eid AA, Fornoni A, Marples B, Zeidan YH. Modulation of radiation-induced damage of human glomerular endothelial cells by SMPDL3B. FASEB J. Epub ahead of print, 2020 2. Hanna J, Bteich M, Tawk Y, Ramadan AH, Asadallah FA, Eid A, Kanj R*, Costantine J*, Eid AA*. Non-invasive, wearable and flexible electromagnetic sensor for continuous glucose monitoring, mimicking vasculature anatomy. Science Advances. Epub ahead of print, 2020 3. Njeim R, Farhat T, Alkhansa S, Youssef N, Dia B, Slika R, Diab R, Azar ST, Eid AA*. Role of the Nox4/AMPK/mTOR signaling axe in adipose inflammation-induced kidney injury. Clin Sci (Lond). 134(4):403-417, 2020. 4. Eid SA, El Massry M, Hichor M, Haddad M, Grenier J, Dia B, Barakat R, Boutary S, Chanal J, Aractingi S, Wiesel P, Szyndralewiez C, Azar ST, Boitard C, Zaatari G, Eid AA*, Massaad C*. Targeting the NADPH Oxidase-4 and Liver X Receptor Signaling Axis Preserve Schwann Cell Integrity in Diabetic Mice. Diabetes. 69(3):448-464, 2020. 5. Noureldein M, Bitar S, Youssef N, Azar ST, Eid AA*. Butyrate Modulates Diabetes-Linked Gut Dysbiosis: Epigenetic and Mechanistic Modifications. J Mol Endocrinol. 64(1):29-42, 2020.

6. Fardoun M, Iratni R, Dehaini H, Eid A, Ghaddar T, El-Elimat T, Alali F, Badran A, Eid AH, Baydoun E. 7-O-methylpunctatin, a Novel Homoisoflavonoid, Inhibits Phenotypic Switch of Human Arteriolar Smooth Muscle Cells. Biomolecules. 8;9(11), 2019. 7. Mohsen-Mroueh F, Noureldein MH, Zeidan Y, Boutary S, Irani S, Eid S, Haddad M, Barakat R, Harb F, Costantine J, Kanj R, Sauleau EA, Ouhtit A, Azar ST, Eid AH, Eid AA*. Unmasking the Interplay Between mTOR and Nox4: Novel Insights into the Mechanism Connecting Diabetes and Cancer. FASEB J. 33: 14051-14066, 2019. 8. Safi R, Mohsen-Kanson T, Nemer G, Dekmak B, Rubeiz N, El-Sabban M, Nassar D, Eid A, Abbas O, Kibbi AG, Kurban M. Loss of ferrochelatase is protective against colon cancer cells: ferrochelatase a possible regulator of the long noncoding RNA H19. J Gastrointest Oncol. 10(5):859-868, 2019. 9. Elkhatib MAW, Mroueh A, Rafeh RW, Sleiman F, Fouad H, Saad EI, Fouda MA, Elgaddar O, Issa K, Eid AH, Eid AA, Abd-Elrahman KS, El-Yazbi AF. Amelioration of perivascular adipose inflammation reverses vascular dysfunction in a model of nonobese prediabetic metabolic challenge: potential role of antidiabetic drugs. Transl Res. S1931-5244(19)30144-6, 2019. 10. Diab El-Harakeh M, Njeim R, Youssef A, Youssef N, Eid AA*, Bouhadir KH*. Novel triazine- based pyrimidines suppress glomerular mesangial cells proliferation and matrix protein accumulation through a ROS-dependent mechanism in the diabetic milieu. Bioorg Med Chem Lett. 1;29(13):1580-1585, 2019. 11. El Zein N, Abdallah MS, Daher CF, Mroueh M, Stephan J, Bahous SA, Eid A, Faour WH. Ghrelin modulates intracellular signaling pathways that are critical for podocyte survival. Cell biochemistry and function. 2019 Jun. 12. Safi R, Mohsen-Kanson T, Nemer G, Dekmak B, Rubeiz N, El-Sabban M, Nassar D, Eid A, Abbas O, Kibbi AG, Kurban M. Loss of ferrochelatase is protective against colon cancer cells: ferrochelatase a possible regulator of the long noncoding RNA H19. Journal of Gastrointestinal Oncology. 2019 Apr 26. 13. Noureldein MH, Dia BA, Nabbouh AI, Eid AA*. Promising anti-diabetic effect of dextran sulfate sodium: Is it its clinical come back?. Diabetes research and clinical practice. 2019 Mar 14. 14. Gerges-Geagea A, Rizzo M, Jurjus A, Capello F, Leone A, Tomasello G, Gracia C, Al-Kattar S, Massade L, Eid AA*. A novel therapeutic approach to colorectal cancer in diabetes: role of Metformin and Rapamycin. Oncotarget. 2019 Feb 12;10(13):1284-1305. 15. Dehaini H, Awada H, El-Yazbi A, Zouein FA, Issa K, Eid AA, Ibrahim M, Badran A, Baydoun E, Pintus G, Eid AH. MicroRNAs as Potential Pharmaco-targets in Ischemia-Reperfusion Injury Compounded by Diabetes. Cells. 2019 Feb;8(2):152. 16. Hammoud S, EL Khansa S, Mahjoub N, Omar A, El-Mass M, Eid AA*. Molecular Basis of the Counteraction by Calcium Channel Blockers of Cyclosporine Nephrotoxicity. American Journal of Physiology – Renal Physiol. 315(3):F572-F582, 2018. 17. Dehaini H, Fardoun M, Saleh HA, El-Yazbi A, Eid AA, Eid AH. Estrogen in vascular smooth muscle cells: A friend or a foe? Vascul Pharmacol. S1537-1891(18)30222-2, 2018. 18. Hamie L, Daoud G, Nemer G, Nammour T, El Chediak A, Uthman IW, Kibbi AG, Eid A, Kurban M. SuPAR, an emerging biomarker in kidney and inflammatory diseases. Postgrad Med J. 0:1– 8, 2018. 19. Noureldein MH, Eid AA*. Gut microbiota and mTOR signaling: Insight on a new pathophysiological interaction. Microb Pathog. 118: 98-104, 2018. 20. J Costantine, Eid A, Abdallah M, Tawk Y, Ramadan AH. A Load Independent Tapered RF Harvester. IEEE Microwave and Wireless Components Letters. 27 (10): 933-35, 2017. 21. Hichor M, Sundaram V, Eid S, Bastin J, Eid AA, Petit P, Grenier J, Borderie D, Massaad C. Liver X Receptor Exerts a Protective Effect Against the Oxidative Stress in the Peripheral Nerve. Scientific Report. 8: 2524, 2018. 22. Abdallah MS, Kennedy CRJ, Stephan JS, Abou Khalil P, Mroueh M, Eid AA, Faour WH. Transforming growth factor-β1 and phosphatases modulate COX-2 protein expression and TAU phosphorylation in cultured immortalized podocytes. Inflamm. Res. 67(2): 191-201, 2018.

23. Hammoud S, Omar A, Eid AA*, El-Mass M*. CYP4A/CYP2C modulation of the interaction of calcium channel blockers with cyclosporine on EDHF-mediated renal vasodilations in rats. Toxicology and Applied Pharmacology. 334: 110-119, 2017. *Corresponding Author(s) 24. Noureldein M, Eid AA*. Homeostatic Effect of Laughter on Diabetic Cardiovascular complications: The myth turned to Fact. Diabetes Research and Clinical Practice. 135:111-119, 2017. *Corresponding Author(s) 25. Abou Daher A, El Jalkh T, Eid AA, Fornoni A, Marples B, Zeidan YH. Translational Aspects of Sphingolipid Metabolism in Renal Disorders. Int. J Mol. Sci. Int J Mol Sci. 18(12), 2017. 26. Gerges Geagea A, Rizzo M, Eid A, Hajj Hussein I, Zgheib Z, Zeenny MN, Jurjus R, Uzzo ML, Spatola GF, Bonaventura G, Leone A, Massaad-Massade L, Jurjus A. Tea Catechins Induce Crosstalk Between Signaling Pathways and Stabilize Mast Cells in Ulcerative Colitis. J Biol Regul Homeost Agents. 31 (4): 865-877, 2017. 27. Abou-El-Hassan H, Dia B, Choucair K, Eid S, Najdi F, Baki L, Talih F, Eid AA*, Kobeissy F*. Traumatic Brain Injury, Diabetic Neuropathy and Altered-Psychiatric Health: The Fateful Triangle. Medical Hypothesis. 108: 69-80, 2017. 28. Pasha M, Eid AH, Eid AA, Gorin Y, Munusamy S. Sestrin2 as a Novel Biomarker and Therapeutic Target for Various Diseases. Oxidative medicine and cellular longevity. Vol. 2017, 3296294, 2017. 29. Hichor M, Kumar Sampathkumar N, Montanaro J, Borderie D, Petit PX, Gorgievski V, Tzavara E, Eid AA, Charbonnier F, Grenier J, Massaad C. Paraquat induces peripheral myelin disruption and locomotor defects: crosstalk with LXR and Wnt pathways. Antioxid Redox Signaling. 27 (3):168-183, 2017. 30. Eid S, Boutary S, Braych K, Sabra R, Hamdy A, Moodad S, Rashid A, Massaad C, Block K, Gorin Y, Abboud HE, and Eid AA*. mTORC2 Signaling Regulates Nox4-Induced Podocyte Depletion in Diabetes. Antioxid Redox Signaling. 25(13):703-719, 2016. 31. Eid S, Massaad C*, and Eid AA*. Oxidative Stress in Diabetic Neuropathy: Strategies for Treatment. Diabetes Case Rep 1:101. doi:10.4172/DCRS.10000101, 2016. 32. Bouhadir KH*, Koubeissi A, Mohsen FA, El-Harakeh MD, Cheaib R, Younes J, Azzi G, Eid AA*. Novel carbocyclic nucleoside analogs suppress glomerular mesangial cells proliferation and matrix protein accumulation through ROS-dependent mechanism in the diabetic milieu. II. Acylhydrazone-functionalized pyrimidines. Bioorg Med Chem Lett. 1;26(3):1020-4, 2016. 33. Nassif J, Abbasi SA , Nassar A , Abu-Musa A and Eid AA*. The Role of NADPH-Derived ROS Production in the Pathogenesis of Endometriosis: A Novel Mechanistic Approach. J Biol Regul Homeost Agents. 30(1): 31-40, 2016. 34. Daoud G, Monzer A, Bahmad H, Chamaa F, Hamdar L, Mouhieddine T, Shayya S, Eid A, Kobeissy F, Liu YN, Abou-Kheir W. Primary versus Castration-Resistant Prostate Cancer: Modeling Through Novel Murine Prostate Cancer Cell Lines. Oncotarget. 7(20): 28961-75, 2016. 35. Jurjus A, Eid A, Al Kattar S, Zeenny MN, Gerges-Geagea A, Haydar H, Hilal A, Oueidat D, Matar M, Tawilah J, Hussein IH, Schembri-Wismayer P, Cappello F, Tomasello G, Leone A, Jurjus RA. Inflammatory bowel disease, colorectal cancer and type 2 diabetes mellitus: The links. BBA Clin. 5:16-24, 2015. 36. Mouhieddine TH, Nokkari A, Itani MM, Chamaa F, Bahmad H, Monzer A, El-Merahbi R, Daoud G, Eid A*, Kobeissy FH*, Abou-Kheir W*. Metformin and Ara-a Effectively Suppress Brain Cancer by Targeting Cancer Stem/Progenitor Cells. Front Neurosci. 23;9: 442, 2015. 37. Abou-Kheir W, Eid A, El-Merahbi R, Assaf R, Daoud G. A Unique Expression of Keratin 14 in a Subset of Trophoblast Cells. PLoS One. 2015 Oct 2;10(10):e0139939. 38. Altara R, Manca M, Sabra R, Eid AA, Booz GW, Zouein FA. Temporal cardiac remodeling post- myocardial infarction: dynamics and prognostic implications in personalized medicine. Heart Fail Rev. 21: 25-47, 2015.

39. El Achkar G, Jouni M, Mrad MF, Hirz T, El Hachem N, Khalaf A, Hammoud S, Fayyad-Kazan H, Eid AA, Badran B, Abou Merhi R, Hachem A, Hamade E, Habib A. Thiazole derivatives as inhibitors of cyclooxygenases in vitro and in vivo. European Journal of Pharmacology. 750: 66– 73, 2015. 40. Eid S, Abdul-Massih C, El-Khuri CM, Hamdy A, Rashid A, Eid AA*. New Mechanistic Insights in The Development of Diabetic Nephropathy: Role of Cytochromes P450 And Their Metabolites. Journal of Endocrine Disorders. 1 (2): 6, 2014. 41. El-Merahbi R, Liu YN, Eid AA, Daoud G, Hosry L, Monzer A, Mouhieddine T, Hamade A, Najjar F, Abou-Kheir W. Berberis Libanotica Ehrenb extracts show anti-neoplastic effects on Prostate Cancer Stem/Progenitor Cells. PLoS One. 9 (11): e112453, 2014.Anwar KM, Abou Kheir W, Eid S, Fares J, Liu X, Eid AH*, Eid AA*. Colorectal and Prostate Cancer Risk in Diabetes: Metformin, an actor behind the scene. the Journal of Cancer. 5(9): 736-744, 2014. 42. Tyan PI, Radwan AH, Eid AA, Haddad AG, Wehbe D, and Taher AT. Novel Approach to Reactive Oxygen Species in Nontransfusion-Dependent Thalassemia. BioMed Research International. 2014:350432, 2014. 43. Hajj-Hussein I, Eid A, Maksoud R, Jambart S, Bou Assi T, Zgheib Z, Oueidat D, Chams N, Chams S, Diab R, Barada K, Jurjus R, Cappello F, Remund J, Kreiker J, Leone A, Jurjus A. Estrogens Control Inflammation in Experimental Colitis. J Biol Regul Homeost Agents. 28(2): 213-224, 2014. 44. Lee DY, Wauquier F, Eid AA, Roman LJ, Ghosh Choudhury G, Khazim K, Block K, Gorin Y. Nox4 NADPH Oxidase Mediates Peroxynitrite-Dependent Uncoupling of Endothelial Nitric Oxide Synthase and Fibronectin Expression in Response to Angiotensin II. Role of Mitochondrial Reactive Oxygen Species. J Biol Chem. J Biol Chem. 288(40):28668-86, 2013. 45. Eid S, Maalouf R, Jaffa A, Nassif J, Ziyadeh FN, Eid AA*. 20-HETE and EETs in Diabetic Nephropathy: A Novel Mechanistic Pathway. PLoS One. 8(8):e70029, 2013. 46. Eid S, Abou-Kheir W, Sabra R, Daoud D, Jaffa A, Ziyadeh FN, Roman L, Eid AA*. Involvment Of Renal Cytochromes P450 And Arachidonic Acid Metabolites in Diabetic Nephropathy. J Biol Regul Homeost Agents. 27(3):693-703, 2013. 47. Ford BM, Eid AA, Gooz M, Barnes JL, Gorin YC, Abboud HE. ADAM17 mediates Nox4 expression and NADPH oxidase activity in the kidney cortex of OVE26 mice. Am J Physiol Renal Physiol. 1;305(3): F323-F332, 2013. 48. Nassif J, Mattar S, Abu Musa A, Eid A. Endometriosis and cancer: what do we know? Minerva Ginecol. 65(2):167-79, 2013. 49. Eid AA, Ford BM, Bhandary B, Cavagliery R, Block K, Barnes JL, Gorin Y, Choudhury GG, Abboud HE. Mammalian Target of Rapamycin Regulates Nox4-Mediated Podocyte Depletion in Diabetic Renal Injury. Diabetes. 62(8):2935-2947, 2013. 50. Eid AA, Lee DY, Roman LJ, Khazim K, Gorin Y. Sestrin 2 and AMPK connect hyperglycemia to Nox4-dependent eNOS uncoupling and matrix protein expression. Mol Cell Biol. 33(17): 3439-3460, 2013. 51. Eid AA*, Koubeissi A, Bou-Mjahed R, Al Khalil N, Farah M, Maalouf R, Bouhadir K*. Novel carbocyclic nucleoside analogs suppress glomerular mesangial cells proliferation and matrix protein accumulation through a ROS-depending mechanism in the diabetic milieu. Bioorg Med Chem Lett. 23(1):174-178, 2013. 52. Nayak BK, Feliers D, Sudarshan S, Friedrichs WE, Day RT, New DD, Fitzgerald JP, Eid A, Denapoli T, Parekh DJ, Gorin Y, Block K. Stabilization of HIF-2α through redox regulation of mTORC2 activation and initiation of mRNA translation. Oncogene. 32(26):3147-3155, 2013. 53. Jaffa MA, Kobeissy F, Al Hariri M, Chalhoub H, Eid AA, Ziyadeh FN, Jaffa AA. Global Renal Gene Expression Profiling Analysis in B2-Kinin Receptor Null Mice: Impact of Diabetes. PLoS ONE 7(9): e44714, 2012. 54. Mrad MF, Mouawad CA, Al-Hariri M, Eid AA, Alam J, Habib A. Statins modulate transcriptional activity of heme-oxygenase-1 promoter in NIH 3T3 cells. J Cell Biochem. 113(11): 3466-3475, 2012.

55. Fitzgerald JP, Nayak B, Shanmugasundaram K, Friedrichs W, Sudarshan S, Eid AA, DeNapoli T, Parekh DJ, Gorin Y, Block K. Nox4 mediates renal cell carcinoma cell invasion through hypoxia-induced interleukin 6- and 8- production. PLoS One. 7(1): e30712, 2012. 56. Maalouf RM#, Eid AA#, Gorin YC, Block K, Escobar GP, Bailey S, Abboud HE. Nox4-derived reactive oxygen species mediate cardiomyocyte injury in early type 1 diabetes. Am J Physiol Cell Physiol. 302(3):C597-604, 2012. (#The authors contributed equally to this work) 57. Eid A, Ford BM, Gorin Y, Block K, Barnes JL, Choudhury GG, Kasinath BK, Abboud HE. AMP- activated Protein Kinase (AMPK) Negatively Regulates Nox4-dependent Activation of p53 and Epithelial Cell Apoptosis in Diabetes. J Biol Chem. 285: 37503 - 37512, 2010. 58. Block K, Parekh D, New D, Chelmicki T, Eid A, Reed R, Choudhury GG, Gorin Y, Abboud HE. NAD(P)H oxidase subunit, p22phox, inhibits the function of the tumor suppressor protein, tuberin. Am. J. Pathology, 176: 2447-2455, 2010. 59. Eid AA, Gorin Y, Fagg BM, Maalouf R, Barnes JL, Block K, Abboud HE. Mechanisms of podocyte injury in diabetes: role of cytochrome P450 and NADPH oxidases. Diabetes. 58:1201- 11, 2009. 60. Block K, Eid A, Griendling KK, Lee DY, Wittrant Y, Gorin Y. Nox4 NAD(P)H oxidase mediates Src-dependent tyrosine phosphorylation of PDK-1 in response to angiotensin II: role in mesangial cell hypertrophy and fibronectin expression. J Biol Chem. 283:24061-76, 2008. 61. Eid A, Bodin S, Ferrier B, Delage H, Boghossian M, Martin M, Baverel G, Conjard A. Intrinsic gluconeogenesis is enhanced in renal proximal tubules of Zucker diabetic fatty rats. J Am Soc Nephrol. 17:398-405, 2006. 62. Guitton J, Conjard A, Eid A, Martin M, Boghossian M, Delage H, Baverel G, Ferrier B. Identification of novel targets of cephaloridine in rabbit renal proximal tubules synthesizing glutamine from alanine. Arch Toxicol.79:587-94, 2005.

Book Chapters

1. Darwish B, Eid AA*. An Overview on the Crosstalk Between the Nervous System and the Kidneys. Morphologia é Clinica. Plumelia edizioni, 2017. *Corresponding Author(s) 2. Jurjus A, Hajj-Hussein I, Eid A. Health Tourism Quality in Lebanon: The First World Health Service at a Third World Cost. ST-7: Education, Services and Public Paper # 7-7K @16-ICT & 8-C&C: 11-13/7/12. Linnaeus University, Vaxjo, Sweden editors: HO, M. Zineldin, F. Soliman, and M. Amsteus. Ppl-7, 2012. (ISBN 978-91-86983-58-1)

Patents

1. Costantine J#, Kanj R#, Eid AA#, Hanna J, Ramadan A, Tawk Y. Antenna Design for Biomarker Monitoring and methods of Use. Provisional Patent Application, Serial No. US 62/690,110, 2018 (#Equal Contribution) 2. Costantine J#, Kanj R#, Eid AA#. A Novel Non-Invasive Biological, Chemical Markers and Tracers Monitoring device in blood including Glucose Monitoring Using RF Circuits and Antenna Design. Provisional Patent Application Serial No. 62/568,658, 2017. (#Equal Contribution) 3. Eid AA, Habib R, Badr K. Determination of Risk for Development of Cardiovascular Disease by Measuring Urinary Levels of Podocin and Nephrin Messenger RNA. International Patent Application Serial No.: PCT/US2015/048983, 2016.

Current Research Fund

Qatar National Research Funds (2020 – 2023) Title: An Interdisciplinary Model Towards Integrative Complementary and Alternative Medicine Use in Qatar and Lebanon: The Case of Breast Cancer and Diabetes Patients Role: Principal Investigator – Diabetes part Budget: $900,000

Medical Practice Plan - American University of Beirut (2020 – 2023) Title: Role of Microbiota in Diabetes Associated Cancer Role: Principal Investigator Budget: $120,000

Council for Australian-Arab Relations (CAAR) (2018 – 2020) Title: Genomics research and technology development in diabetes complications Role: Principal Investigator Budget: $60,000/year

United Kingdom-Lebanon Tech Hub (UKLTH) (2017 – 2020) Title: A Novel Non-Invasive Glucose Monitoring Device Role: Principal Investigator Budget: $1,150,000

Qatar National Research Funds (2015 – 2020) Title: Sestrin 2 as a novel therapeutic target and biological marker in diabetic nephropathy Role: Co-Principal Investigator Budget: $890,000

International Diabetic Neuropathy Consortium (IDNC) (2017 – 2022) The International Diabetic Neuropathy Consortium (IDNC) was established in May 2015, which was made possible thanks to a grant from the Novo Nordisk Foundation. Role: The Eid lab at the American University of Beirut - Faculty of Medicine joined this venture as an International collaborator

Previous Research Fund

Medical Practice Plan - American University of Beirut (2017 – 2019) Title: Mechanisms of Schwann Cells Injury in Diabetes: Unveiling New Mechanistic Pathways for Innovative Therapeutic Approaches Role: Principal Investigator Budget: $120,000

CEDRE (2015 – 2017) Title: Role of oxysterols and Reactive Oxygen Species (ROS) in Diabetic Neuropathy Role: Principal Investigator Budget: €20,000

Lebanese National Council for Scientific Research (2015 – 2017) Title: Involvement of renal cytochromes P450 and their metabolites in diabetic Renal Injury Role: Principal Investigator Budget: $25,000

Farouk Jabr Award, American University of Beirut (2017 – 2019) Title: Phosphorus Alteration in Diabetic Nephropathy and Diabetic Cardiomyopathy: Unveiling Novel Mechanistic Pathways Role: Principal Investigator Budget: $25,000

Medical Practice Plan - American University of Beirut (2016 – 2019) Title: Novel Biomarkers for the Detection of Early Onset of Diabetic Nephropathy and Cardiovascular Outcomes Role: Co- Principal Investigator Budget: $120,000

Medical Practice Plan - American University of Beirut (2016 – 2019) Title: Podocyturia mRNAs: earlier and superior predictors of cardiovascular outcomes in diabetic and non-diabetic hypertensive patients Role: Co- Principal Investigator Budget: $120,000

Lebanese National Council for Scientific Research (2016 – 2019) Title: Novel Synthetic Nucleosides Analogs: Potential Antioxidants for the Management of Diabetic Complications Role: Co- Principal Investigator Budget: $40,000

Collaborative Research Stimulus, American University of Beirut (2018 – 2019) Title: Study of the Role of Rb, and Combined role of Rb and p53 pathways, during kidney development and nephrogenesis Role: Co- Principal Investigator Budget: $32,000

Collaborative Research Stimulus, American University of Beirut (2018 – 2019) Title: Does Hepatic Heating Control Eating? Role: Co- Principal Investigator Budget: $32,000

Alpha Omega Alpha Carolyn L. Kuckein - Student Fellowship Award (2016) Title: MicroRNA-based regulation of Cytochrome P 450 enzymes as a novel approach in the management of diabetic nephropathy Role: Mentor/Supervisor

Qatar National Research Funds (2012 – 2017) Title: Mechanism of epithelial Cells Injury in Diabetes Role: Principal Investigator Budget: $1,128,000

Qatar National Research Funds (2012 – 2017) Title: AMPK, mTOR and Tuberin: Novel Biological Pathways Linking Diabetes to Colorectal Cancer Role: Principal Investigator Budget: $1,000,000

Juvenile Diabetes Research Foundation (2010 – 2013) Title: Mechanisms of Epithelial Cells Injury: NADPH oxidases and Diabetic Nephropathy Role: Principal Investigator Budget: $128,000

Farouk Jabr Award, American University of Beirut (2013-2015) Title: Synthesis & Biological study of novel carbocyclic nucleoside analogs Role: Principal Investigator Budget: $25,000

Medical Practice Plan - American University of Beirut (2013 – 2016) Title: Diabetic Nephropathy: Involvement of Renal Cytochromes P450 and Their Metabolites Role: Principal Investigator Budget: $100,000

Medical Practice Plan - American University of Beirut (2011 - 2013) Title: Glomerular Epithelial Cell Injury in Diabetes: A Novel Crosstalk Between AMPK and NADPH Role: Principal Investigator Budget: $120,000

Lebanese National Council for Scientific Research (2011 – 2013) Title: Mechanisms of Kidney Cell Injury in diabetes Role: Principal Investigator Budget: $36,000

Medical Practice Plan - American University of Beirut (2011 – 2013) Title: Neural regulation of glucose absorption in rat jejunum via sglt1 and glut2: dependence on intraluminal glucose concentration and potential implications for obesity and diabetes Role: Co-Principal Investigator Budget: $120,000

Medical Practice Plan - American University of Beirut (2011 – 2013) Title: Molecular and Cellular Mechanisms of Diabetic Nephropathy Role: Co-Principal Investigator Budget: $120,000

Medical Practice Plan - American University of Beirut (2013 – 2014)

Title: Inhibitory reflex of glucose on its own absorption from a distant intestinal site: role of artificial sweeteners and type 2 diabetes mellitus Role: Co-Principal Investigator Budget: $40,000

Lebanese National Council for Scientific Research (2013 – 2015) Title: Preparation of potentially active hydrazono-carbanucleosides for diabetic nephropathy Role: Co-Principal Investigator Budget: $36,000

Editorial Board

• Journal of Endocrine Disorders • Journal of Diabetes & Metabolism • Integrative Obesity and Diabetes (IOD) • Diabetes Case Reports

Reviewer Activity

1. Grants Scientific Study Section - MPP – American University of Beirut - Sultan Qabous Research Committee - Lebanese National council for Scientific research

2. AdHoc manuscript Reviewer - American Journal of Physiology - Scientific Report - Diabetes - FASEB - Diabetologia - Hemoglobin - Plos One - Molecular neurobiology - Molecular Biology Reports - Bioinformatics and Biology Insights. - Diabetes and Vascular Disease Research

Teaching Activity

Since I joined AUB, in November of 2010, I was involved in developing, implementing, coordinating and teaching medical, graduate and undergraduate courses.

A. Course Development and Implementation

1. Impact Curriculum – Faculty of Medicine Since joining AUB, and as member of PRIME “Program for Research and Innovation in Medical Education”, I had a major contribution in the new curriculum that was developed and implemented in 2013-2014 in our medical school “The Impact Curriculum”. I was responsible for developing and implementing a new interdisciplinary, integrated, basic science course “Cellular and Molecular Basis of Medicine” as well as I actively contributed in the Development of a new interdisciplinary, integrated basic science and clinical course “Renal Module”.

Cellular and Molecular Basis of Medicine – IDTH 201 1) Course Description: “The Cellular and Molecular Basis of Medicine” is an interdisciplinary, integrated, basic science course which draws upon the disciplines of histology, cell and molecular biology, cell physiology, biochemistry and pharmacology to present the basic concepts and principles that underlie the normal structure and function of the human body at the cellular and molecular level”.

2) Credits and Audience: This is a 7 credits course that is tailored for Med 1 students.

3) Implementation Date: Fall of 2013 – 2014.

Renal Module - IDTH 228

1) Course Description: “The Renal module” is an integrated course that covers the anatomy, histology, physiology, pathology, pathophysiology and pharmacology related to the kidneys and urinary system, and the application of this knowledge in solving related clinical problems.

2) Credits and Audience: This is a 4 credits course that is tailored for Med 2 students.

3) Implementation Date: Fall of 2014 - 2015.

2. Graduate Program

During the last year 2013-2014, major changes were implemented in the graduate program of the faculty of Medicine at AUB. The “General Physiology: Cellular Mechanisms” and homeostasis” courses were totally redesigned to fit the research-oriented needs lectures and discussions of graduate students. New topic, a series of discussion, paper presentations, research techniques and methodology, covering the basic, translational and clinical research areas of the various topics of these 2 courses were introduces.

General Physiology: Cellular Mechanisms - PHYL 310

1) Course Description: The “General Physiology: Cellular Mechanisms” course describes and highlights the aspects of membrane transport processes across symmetrical and asymmetrical cell membranes, electrophysiology, membrane potentials, action potentials in excitable cells, synaptic transmissions, and excitation-contraction coupling in muscles.

2) Credits and Audience: This is a 4 credits core course that is offered to Ph.D. and masters graduate students.

3) Implementation Date: Fall of 2013 - 2014.

Homeostasis - PHYL 300

1) Course Description: The “Homeostasis” course studies the internal environment and its physiological regulation by two homeostatic organs: the lungs and the kidneys. Didactic lectures cover the physiology of the topic, treating internal environment, homeostasis and feedback mechanisms, the lung, the kidney, and electrolytes

2) Credits and Audience: This is a 2 credits course that is offered to Ph.D. and masters graduate students.

3) Implementation Date: Spring of 2013 - 2014.

3. Undergraduate Program - Nursing

I was appointed by the chair of the Department of Anatomy, Cell Biology and Physiological Sciences, to head a committee charged to redesign the “Human Morphology for Paramedical and Undergraduate Students” (HUMR 246) and the “Human Physiology for Paramedical and Undergraduate Students” (PHYL 246) courses. The results of the work were the creation of new courses that will replace the histology and physiology courses for nursing. HUMR 244 “Introduction to Human Biology” and HUMR 248 “Human Anatomy and Physiology” will be offered to the nursing students of the Hariri School of Nursing starting this year.

Introduction to Human Biology - HUMR 244

1) Course Description: The “Introduction to Human Biology” course is an introductory course that meets the needs of a diverse group of students who are preparing for careers in allied health sciences, medical technologies or other non-medical careers like psychology or biomedical sciences. It introduces the students to the very basic terms and concepts in anatomy, histology and physiology. The course covers the basic biology of the cells, tissues and organs of the human body

2) Credits and Audience: This is a 2 credits core course that is offered to nursing undergraduate students and other paramedical, undergraduate students.

3) Implementation Date: Fall of 2014 – 2015.

Human Anatomy and Physiology - HUMR 248

1) Course Description: The “Human Anatomy and Physiology” course provides a strong foundation for understanding the structural complexities of the human organism and related physiological functions. The course, as conceived, will integrate structure and function and offer practical advantages in fine-tuning the balance between anatomy, histology and physiology. In addition, clinical correlations will be included for a vertical integration in addition to the horizontal integration.

2) Credits and Audience: This is a 5 credits course that is offered to nursing undergraduate students as well as to other undergraduate students.

3) Implementation Date: Annually; 2014 - 2015.

B. Course Coordination

As a coordinator, I am responsible for the development and innovation as well as day-to- day operation of different courses that were offered to Medical, graduate and undergraduate students.

Medical courses. • Cellular and Molecular Basis of Medicine - IDTH 201; 7 credits (Coordinator) • Renal Module - IDTH 228; 4 credits (Coordinator)

Graduate courses. • General Physiology, Cellular Mechanisms – PHYL 310; 3 credits (Coordinator) • Homeostasis - PHYL 300; 2 credits (Coordinator) • Cardiovascular Physiology – PHYL 302; 2 credits (Coordinator)

Undergraduate courses (Paramedical). Ø Human Physiology for Paramedical and Undergraduate Students - PHYL 246; 4 credits (Co-Coordinator)

C. Course Teaching

Involved in teaching the following courses in the Medical, Graduate and Undergraduate Programs:

Teaching Medical courses

Cellular and Molecular Basis of Medicine - IDTH 201; 7 credits

1) Lectures taught: • Introduction to Homeostasis • Cell Structure and Cell function • Receptors and signaling • Epithelia and transport • Channels • Transporters • Body fluids and cell volume regulation

2) Laboratory sessions: • Epithelial tissue Histology

3) Team Based Learning (TBL) session: • Epithelial Transport, Cell Volume and Body fluids.

4) Audience: • Med 1 students

Renal Module (Med 2) - IDTH 228; 3 credits

1) Lectures that will be taught: • Histology of kidney and urinary tract • Renal sodium transport • Renal water transport.

2) Laboratory sessions: • Kidney and urinary tract histology

3) Audience: • Med 2 students

Teaching Graduate courses

General Physiology: Cellular Mechanisms - PHYL 300; 3 credits

1) Lectures taught:

• Homeostasis • Cell Function • Epithelia • Signal transduction • Ion Channels • Transporters • Transport Across membranes • Body Fluid Compartment • Cell Volume Regulation

2) TBL and discussion sessions: • Cellular Function • Membrane Phenomena and Signal Transduction • Epithelial transport and channelopathy • Transport Across Epithelial Tissues • Cell Volume Regulation • Presentations