DOCSLIB.ORG

Cannibalism in the Brown Marmorated Stink Bug Halyomorpha Halys (Stål)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Cannibalism in the Brown Marmorated Stink Bug Halyomorpha Halys (Stål) insects Brief Report Cannibalism in the Brown Marmorated Stink Bug Halyomorpha halys (Stål) Giulia Papa and Ilaria Negri * Department of Sustainable Crop Production, Università Cattolica del Sacro Cuore Via Emilia Parmense 84, 29122 Piacenza, Italy; [email protected] * Correspondence: [email protected] Received: 7 August 2020; Accepted: 16 September 2020; Published: 19 September 2020 Simple Summary: Overwintering populations of the crop pest Halyomorpha halys exhibit cannibalistic behaviour towards conspecifics. Depletion of metabolic reserves and desiccation occurring in winter can be overcome by intraspecific predation. This behaviour may be facilitated by the aggregation of individuals and the suppression of species-specific signals that prevent predation upon conspecifics. Abstract: The phytophagous brown marmorated stink bug Halyomorpha halys (Stål) is known to exhibit cannibalistic behaviour towards eggs. Here, we provide evidence of cannibalism among overwintering H. halys adults. Since diapausing individuals have high physiological demands for surviving long periods under stressful conditions, including the risk of depletion of metabolic reserves and desiccation, we assumed that nutritional and water requirements can be met by intraspecific predation. The role of aggregative behaviour in promoting cannibalism is also discussed. Given its evolutionary advantage, this trait should be maintained over generations and may be more widespread than previously considered in species that display aggregative behaviour during adverse seasons. Keywords: cannibalism; Halyomorpha halys; Hemiptera; Pentatomidae; overwintering; phytophagous insect; diapause; aggregation 1. Introduction The brown marmorated stink bug Halyomorpha halys (Stål, 1855) (Hemiptera: Pentatomidae) is native to East Asia, but has been spreading in Europe since 2004, first in Switzerland and then in the rest of Europe [1,2]. This species is a significant invasive agricultural pest that damages fruits trees (e.g., apples, peaches, and pears), vegetables, row crops, and ornamental plants [3,4]. H. halys feeds by penetrating a stylet bundle into plant tissues and injecting digestive enzymes to allow sucking of plant fluids, resulting in feeding injuries in the plant tissues, such as deformities, scars, discolouration, and pitting [4]. H. halys overwinter as adults and exhibit aggregative behaviour in thermally buffered microhabitats [5]. Aggregated individuals then enter facultative diapause for several months until specific environmental cues in spring trigger their re-emergence. For example, in northern Italy, adults exit diapause when the maximum temperature exceeds 14 ◦C and the photoperiod has 13 h of light [6]. Adults may become active through winter in response to warm temperatures and a poor nutritional state [7,8]. The metabolic strategies and physiological mechanisms used by H. halys to survive during winter diapause are not fully known [7]. According to previous studies, depletion of energy reserves and desiccation are the main causes of mortality in the overwintering generation, and adequate nutrition and sequestration of energy reserves (e.g., lipids, glycogen, and sugars) in individuals before entering diapause majorly influence diapause success [7–9]. Insects 2020, 11, 643; doi:10.3390/insects11090643 www.mdpi.com/journal/insects Insects 2020, 11, x FOR PEER REVIEW 2 of 7 nutrition and sequestration of energy reserves (e.g., lipids, glycogen, and sugars) in individuals before entering diapause majorly influence diapause success [7–9]. Overwintering populations of herbivorous species displaying aggregative habits usually have few or no sources of food, other than dead conspecifics [10]. Conspecific necrophagy has been documented among overwintering adults, for example, in the boxelder bug Leptocoris trivittatus [10]. InsectsNecrophagy2020, 11, 643does not occur in H. halys, and freshly dead individuals can even repel conspecifics 2[11]. of 7 Here, we provide evidence that supports the hypothesis of cannibalism, i.e., feeding on live conspecifics, among overwintering adults of H. halys. Cannibalism is defined as intraspecific predationOverwintering and has been populations observed ofin herbivorousmany animals, species including displaying protozoa, aggregative insects, birds, habits and usually mammals have few[12]. orCannibalism no sources may of food, play otherdifferent than roles dead in conspecifics the ecology [10of ].a population, Conspecific such necrophagy as stabilising has been the documentedhost–plant/insect among relationship, overwintering regulating adults, population for example, density, in the boxelderand providing bug Leptocoris a selective trivittatus advantage[10]. Necrophagyby removing doesconspecifics not occur that in H.are halys infected, and with freshly pathogens, dead individuals which reduces can even the repel rates conspecifics of parasitism [11 in]. subsequentHere, we generations provide evidence[13]. Cann thatibalistic supports behaviour the hypothesis may be influe of cannibalism,nced by both i.e., density-dependent feeding on live conspecifics,and -independent among factors; overwintering for example, adults developmenta of H. halys. Cannibalisml asynchrony is with defined the ashost intraspecific plant, sex or predation genetic andrelatedness has been of the observed participants, in many and animals, limitations including in the amount protozoa, or quality insects, of birds, food andand mammalsenvironmental [12]. Cannibalismfactors, such as may humidity play diandfferent high rolestemperature in the [13]. ecology of a population, such as stabilising the host–plantAlthough/insect cannibalism relationship, has regulatingbeen observed population in predatory density, insects, and providing it has also a been selective documented advantage in bymany removing phytophagous conspecifics species, that areincluding infected member with pathogens,s of Lepidoptera, which reduces Coleoptera, the rates and of parasitism Hemiptera in subsequent[12,13]. In phytophagous generations [13 hemipter]. Cannibalisticans, cannibalism behaviour has may been be influencedreported in byadults both and density-dependent nymphs which andpredate -independent on eggs, factors;e.g., Parastrachia for example, japonensis developmental, Nezara asynchrony viridula, withand theH. hosthalys plant, [14–17], sex orwhereas genetic relatednesscannibalism ofamong the participants, adults has only and been limitations suggested in the but amount never documented or quality of [16]. food and environmental factors, such as humidity and high temperature [13]. 2. MaterialsAlthough and cannibalism Methods has been observed in predatory insects, it has also been documented in many phytophagous species, including members of Lepidoptera, Coleoptera, and Hemiptera [12,13]. During November 2017, about 400 H. halys adults in diapause at aggregation sites were In phytophagous hemipterans, cannibalism has been reported in adults and nymphs which predate on collected from barns in the Province of Cremona (northern Italy, 45°08′17.3′′ N, 10°26′10.9′′ E). After eggs, e.g., Parastrachia japonensis, Nezara viridula, and H. halys [14–17], whereas cannibalism among removing dead/damaged individuals, 215 live H. halys adults were selected and placed into a single adults has only been suggested but never documented [16]. cage (ca. 40 cm high × 20 cm diameter) filled with cardboard panels. The cage was kept under 2.outdoor Materials conditions, and Methods following the protocol adopted by Costi et al. [6]. The individuals were maintained in diapause without any food or water for about 14 weeks. In mid-February 2018, diapauseDuring was November terminated 2017, by about exposure 400 H. halysto 25adults °C/L16:D8 in diapause to start at aggregation laboratory sitescolonies. were collectedAmong fromindividuals barns in that the had Province just exited of Cremona diapause, (northern we ob Italy,served 45◦ 08one017.3 living00 N, 10adult◦26 010.9being00 E).cannibalised After removing by a deadconspecific/damaged (Figure individuals, 1A; Video 215S1). liveThisH. observation halys adults was were shortly selected followed and by placed two other into occurrences a single cage of (ca. 40 cm high 20 cm diameter) filled with cardboard panels. The cage was kept under outdoor this behaviour (Videos× S2 and S3). conditions,Dead specimens following left the in protocol the overwintering adopted by cage Costi were et al.counted [6]. The and individuals the bodies inspected were maintained using a instereomicroscope diapause without to check any food for the or presence water for of about predation 14 weeks. marks. In Scanning mid-February electron 2018, microscopy diapause (SEM; was terminatedZeiss Gemini by exposureSEM 500) to 25was◦C /usedL16:D8 to to confirm start laboratory the occurrence colonies. Amongof predation individuals marks. that For had SEM just exitedobservations, diapause, naturally we observed dehydrated one living specimens adult being were cannibalised gold-coated by and a conspecific mounted onto (Figure a carbon-coated1A; Video S1). Thisstub. observation was shortly followed by two other occurrences of this behaviour (Videos S2 and S3). Figure 1. Prey and predators of Halyomorpha halys.(. (A) Frame Frame of Video Video S1 showing cannibalism in the metathoracic ventral part of prey. (B) PicturePicture showingshowing predation in dorsaldorsal partpart ofof prey.prey. MovementMovement responses ofof preyprey proddedprodded withwith aa brushbrush demonstrateddemonstrated thatthat
Load more

Recommended publications
  • Maternal Care in Acanthosomatinae (Insecta: Heteroptera: Acanthosomatidae)̶Correlated Evolution with Title Morphological Change
    Maternal care in Acanthosomatinae (Insecta: Heteroptera: Acanthosomatidae)̶correlated evolution with Title morphological change Author(s) Tsai, Jing-Fu; Kudo, Shin-ichi; Yoshizawa, Kazunori BMC Evolutionary Biology, 15, 258 Citation https://doi.org/10.1186/s12862-015-0537-4 Issue Date 2015-11-19 Doc URL http://hdl.handle.net/2115/63251 Rights(URL) http://creativecommons.org/licenses/by/4.0 Type article File Information 10.1186_s12862-015-0537-4.pdf Instructions for use Hokkaido University Collection of Scholarly and Academic Papers : HUSCAP Tsai et al. BMC Evolutionary Biology (2015) 15:258 DOI 10.1186/s12862-015-0537-4 RESEARCH ARTICLE Open Access Maternal care in Acanthosomatinae (Insecta: Heteroptera: Acanthosomatidae)— correlated evolution with morphological change Jing-Fu Tsai1,3*, Shin-ichi Kudo2 and Kazunori Yoshizawa1 Abstract Background: Maternal care (egg-nymph guarding behavior) has been recorded in some genera of Acanthosomatidae. However, the origin of the maternal care in the family has remained unclear due to the lack of phylogenetic hypotheses. Another reproductive mode is found in non-caring species whose females smear their eggs before leaving them. They possess pairs of complex organs on the abdominal venter called Pendergrast’s organ (PO) and spread the secretion of this organ onto each egg with their hind legs, which is supposed to provide a protective function against enemies. Some authors claim that the absence of PO may be associated with the presence of maternal care. No study, however, has tested this hypothesis of a correlated evolution between the two traits. Results: We reconstructed the molecular phylogeny of the subfamily Acanthosomatinae using five genetic markers sequenced from 44 species and one subspecies with and without maternal care.
    [Show full text]
  • (Pentatomidae) DISSERTATION Presented
    Genome Evolution During Development of Symbiosis in Extracellular Mutualists of Stink Bugs (Pentatomidae) DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Alejandro Otero-Bravo Graduate Program in Evolution, Ecology and Organismal Biology The Ohio State University 2020 Dissertation Committee: Zakee L. Sabree, Advisor Rachelle Adams Norman Johnson Laura Kubatko Copyrighted by Alejandro Otero-Bravo 2020 Abstract Nutritional symbioses between bacteria and insects are prevalent, diverse, and have allowed insects to expand their feeding strategies and niches. It has been well characterized that long-term insect-bacterial mutualisms cause genome reduction resulting in extremely small genomes, some even approaching sizes more similar to organelles than bacteria. While several symbioses have been described, each provides a limited view of a single or few stages of the process of reduction and the minority of these are of extracellular symbionts. This dissertation aims to address the knowledge gap in the genome evolution of extracellular insect symbionts using the stink bug – Pantoea system. Specifically, how do these symbionts genomes evolve and differ from their free- living or intracellular counterparts? In the introduction, we review the literature on extracellular symbionts of stink bugs and explore the characteristics of this system that make it valuable for the study of symbiosis. We find that stink bug symbiont genomes are very valuable for the study of genome evolution due not only to their biphasic lifestyle, but also to the degree of coevolution with their hosts. i In Chapter 1 we investigate one of the traits associated with genome reduction, high mutation rates, for Candidatus ‘Pantoea carbekii’ the symbiont of the economically important pest insect Halyomorpha halys, the brown marmorated stink bug, and evaluate its potential for elucidating host distribution, an analysis which has been successfully used with other intracellular symbionts.
    [Show full text]
  • First Records of Halyomorpha Halys (Stål, 1855) (Hemiptera: Heteroptera: Pentatomidae) in Vorarlberg and Vienna, Austria
    ©Österr. Ges. f. Entomofaunistik, Wien, download unter www.zobodat.at Beiträge zur Entomofaunistik 16: 115–139 From the west and from the east? First records of Halyomorpha halys (STÅL, 1855) (Hemiptera: Heteroptera: Pentatomidae) in Vorarlberg and Vienna, Austria. Aus dem Westen und dem Osten? Erste Nachweise von Halyomorpha halys (STÅL, 1855) (Hemiptera: Heteroptera: Pentatomidae) in Vorarlberg und Wien, Österreich. The Brown Marmorated Stink Bug, Halyomorpha halys (STÅL, 1855), is native to East Asia (China, Taiwan, Japan, Korea, Vietnam) (LEE & al. 2013). It was first discovered outside its native distribution range in North America in the mid-1990s (HOEBEKE & CARTER 2003) and has spread to more than 40 U.S. federal states and Canada (Ontario) since then. The first record in Europe dates back to 2004, when specimens were found in Liechtenstein (ARNOLD 2009). Halyomorpha halys was subsequently recorded in several cantons in Switzerland (e.g., WERMELINGER & al. 2008, WYNIGER & KMENT 2010, HAYE & al. 2014a), southern Germany (HEckMANN 2012) and northeastern regions of France (CALLOT & BRUA 2013). In 2012 it was detected in Modena, northern Italy (MAISTRELLO & al. 2013), and until 2014 approximately 200 records were made in northern Italy (MAISTRELLO & al. 2014). Genetic data indicate that Italian populations derive from at least two independent introduction events, one from Switzerland and one from Asia or North America (CESARI & al. 2015). In 2013 H. halys was detected in France in the region Île-de-France, some 400 km further west (GARROUSTE & al. 2014), and in Hungary in the vicinity of Budapest (VÉTEK & al. 2014), several hundred kilometres away from the closest known records in Italy.
    [Show full text]
  • EPPO Reporting Service
    ORGANISATION EUROPEENNE EUROPEAN AND MEDITERRANEAN ET MEDITERRANEENNE PLANT PROTECTION POUR LA PROTECTION DES PLANTES ORGANIZATION EPPO Reporting Service NO. 1 PARIS, 2021-01 General 2021/001 New data on quarantine pests and pests of the EPPO Alert List 2021/002 Update on the situation of quarantine pests in the Russian Federation 2021/003 Update on the situation of quarantine pests in Tajikistan 2021/004 Update on the situation of quarantine pests in Uzbekistan 2021/005 New and revised dynamic EPPO datasheets are available in the EPPO Global Database Pests 2021/006 Anoplophora glabripennis eradicated from Austria 2021/007 Popillia japonica is absent from Germany 2021/008 First report of Scirtothrips aurantii in Spain 2021/009 Agrilus planipennis found in Saint Petersburg, Russia 2021/010 First report of Spodoptera frugiperda in Syria 2021/011 Spodoptera frugiperda found in New South Wales, Australia 2021/012 Spodoptera ornithogalli (Lepidoptera Noctuidae - yellow-striped armyworm): addition to the EPPO Alert List 2021/013 First report of Xylosandrus compactus in mainland Spain 2021/014 First report of Eotetranychus lewisi in mainland Portugal 2021/015 First report of Meloidogyne chitwoodi in Spain 2021/016 Update on the situation of the potato cyst nematodes Globodera rostochiensis and G. pallida in Portugal Diseases 2021/017 First report of tomato brown rugose fruit virus in Belgium 2021/018 Update on the situation of tomato brown rugose fruit virus in Spain 2021/019 Update on the situation of Acidovorax citrulli in Greece with findings
    [Show full text]
  • Border Habitat Effects on Captures of Halyomorpha Halys
    insects Article Border Habitat Effects on Captures of Halyomorpha halys (Hemiptera: Pentatomidae) in Pheromone Traps and Fruit Injury at Harvest in Apple and Peach Orchards in the Mid-Atlantic, USA James Christopher Bergh 1,*, William R. Morrison III 2 , Jon W. Stallrich 3, Brent D. Short 4, John P. Cullum 5 and Tracy C. Leskey 5 1 Virginia Tech, Alson Smith, Jr. Agricultural Research and Extension Center, Winchester, VA 22602, USA 2 United States Department of Agriculture Agricultural Research Service, Center for Animal Health and Grain Research, Manhattan, KS 66502, USA; [email protected] 3 Department of Statistics, Carolina State University, Raleigh, NC 27695, USA; [email protected] 4 Trécé, Inc., Adair, OK 74330, USA; [email protected] 5 United States Department of Agriculture Agricultural Research Service, Appalachian Fruit Research Station, Kearneysville, WV 25430, USA; [email protected] (J.P.C.); [email protected] (T.C.L.) * Correspondence: [email protected]; Tel.: +1-540-232-6046 Simple Summary: Brown marmorated stink bug (BMSB) is a significant threat to the production of tree fruit, corn and soybean, and some vegetable crops in much of the USA and abroad. Its feeding causes injury that reduces crop quality and yield. BMSB invades crop fields from adjoining habitats, Citation: Bergh, J.C.; Morrison, W.R., where it also feeds and develops on a broad range of wild and cultivated plants. Thus, it is considered III; Stallrich, J.W.; Short, B.D.; Cullum, a perimeter-driven threat, and research on management tactics to reduce insecticide applications J.P.; Leskey, T.C. Border Habitat against it has focused on intervention at crop edges.
    [Show full text]
  • Assessing the Distribution of Exotic Egg Parasitoids of Halyomorpha Halys in Europe with a Large-Scale Monitoring Program
    insects Article Assessing the Distribution of Exotic Egg Parasitoids of Halyomorpha halys in Europe with a Large-Scale Monitoring Program Livia Zapponi 1 , Francesco Tortorici 2 , Gianfranco Anfora 1,3 , Simone Bardella 4, Massimo Bariselli 5, Luca Benvenuto 6, Iris Bernardinelli 6, Alda Butturini 5, Stefano Caruso 7, Ruggero Colla 8, Elena Costi 9, Paolo Culatti 10, Emanuele Di Bella 9, Martina Falagiarda 11, Lucrezia Giovannini 12, Tim Haye 13 , Lara Maistrello 9 , Giorgio Malossini 6, Cristina Marazzi 14, Leonardo Marianelli 12 , Alberto Mele 15 , Lorenza Michelon 16, Silvia Teresa Moraglio 2 , Alberto Pozzebon 15 , Michele Preti 17 , Martino Salvetti 18, Davide Scaccini 15 , Silvia Schmidt 11, David Szalatnay 19, Pio Federico Roversi 12 , Luciana Tavella 2, Maria Grazia Tommasini 20, Giacomo Vaccari 7, Pietro Zandigiacomo 21 and Giuseppino Sabbatini-Peverieri 12,* 1 Centro Ricerca e Innovazione, Fondazione Edmund Mach (FEM), Via Mach 1, 38098 S. Michele all’Adige, TN, Italy; [email protected] (L.Z.); [email protected] (G.A.) 2 Dipartimento di Scienze Agrarie, Forestali e Alimentari, University di Torino (UniTO), Largo Paolo Braccini 2, 10095 Grugliasco, TO, Italy; [email protected] (F.T.); [email protected] (S.T.M.); [email protected] (L.T.) 3 Centro Agricoltura Alimenti Ambiente (C3A), Università di Trento, Via Mach 1, 38098 S. Michele all’Adige, TN, Italy 4 Fondazione per la Ricerca l’Innovazione e lo Sviluppo Tecnologico dell’Agricoltura Piemontese (AGRION), Via Falicetto 24, 12100 Manta, CN,
    [Show full text]
  • Key for the Separation of Halyomorpha Halys (Stål)
    Key for the separation of Halyomorpha halys (Stål) from similar-appearing pentatomids (Insecta : Heteroptera : Pentatomidae) occuring in Central Europe, with new Swiss records Autor(en): Wyniger, Denise / Kment, Petr Objekttyp: Article Zeitschrift: Mitteilungen der Schweizerischen Entomologischen Gesellschaft = Bulletin de la Société Entomologique Suisse = Journal of the Swiss Entomological Society Band (Jahr): 83 (2010) Heft 3-4 PDF erstellt am: 11.10.2021 Persistenter Link: http://doi.org/10.5169/seals-403015 Nutzungsbedingungen Die ETH-Bibliothek ist Anbieterin der digitalisierten Zeitschriften. Sie besitzt keine Urheberrechte an den Inhalten der Zeitschriften. Die Rechte liegen in der Regel bei den Herausgebern. Die auf der Plattform e-periodica veröffentlichten Dokumente stehen für nicht-kommerzielle Zwecke in Lehre und Forschung sowie für die private Nutzung frei zur Verfügung. Einzelne Dateien oder Ausdrucke aus diesem Angebot können zusammen mit diesen Nutzungsbedingungen und den korrekten Herkunftsbezeichnungen weitergegeben werden. Das Veröffentlichen von Bildern in Print- und Online-Publikationen ist nur mit vorheriger Genehmigung der Rechteinhaber erlaubt. Die systematische Speicherung von Teilen des elektronischen Angebots auf anderen Servern bedarf ebenfalls des schriftlichen Einverständnisses der Rechteinhaber. Haftungsausschluss Alle Angaben erfolgen ohne Gewähr für Vollständigkeit oder Richtigkeit. Es wird keine Haftung übernommen für Schäden durch die Verwendung von Informationen aus diesem Online-Angebot oder durch das
    [Show full text]
  • Host Orientation Using Volatiles in the Phoretic Nematode Caenorhabditis
    © 2014. Published by The Company of Biologists Ltd | The Journal of Experimental Biology (2014) 217, 3197-3199 doi:10.1242/jeb.105353 SHORT COMMUNICATION Host orientation using volatiles in the phoretic nematode Caenorhabditis japonica Etsuko Okumura1,2,* and Toyoshi Yoshiga1,3,‡ ABSTRACT host-biased phoresy with the burrower bug Parastrachia japonensis Host orientation is the most important step in host-searching Scott (Kiontke et al., 2002; Yoshiga et al., 2013). Dauer larvae (DL), nematodes; however, information on direct cues from hosts to evoke a developmentally arrested and phoretic stage of the nematode and this behaviour is limited. Caenorhabditis japonica establishes a a developmental analogue of IJs in EPNs, are predominantly found species-specific phoresy with Parastrachia japonensis. Dauer larvae on P. japonensis adult females throughout the year in fields. Only (DL), the non-feeding and phoretic stage of C. japonica, are the nematode benefits from this phoresy. The nematode uses the host predominantly found on female phoretic hosts, but the mechanisms insect to prolong its own survival (Tanaka et al., 2012) and for underlying the establishment of this phoresy remain unknown. To transport to food resources, such as the nest of the host insect where determine whether C. japonica DL are able to recognize and orient the mother insect stores fruits of Schoepfia jasminodora Siebold & themselves to a host using a volatile cue from the host, we developed Zuccarini for her nymphs, as well as eggs and nymphal carcasses a Y-tube olfactory assay system in which C. japonica DL were (Okumura et al., 2013b; Yoshiga et al., 2013). Thus, the phoresy is significantly attracted to the air from P.
    [Show full text]
  • Discrimination Between Eggs from Stink Bugs Species in Europe Using MALDI-TOF MS
    insects Article Discrimination between Eggs from Stink Bugs Species in Europe Using MALDI-TOF MS Michael A. Reeve 1,* and Tim Haye 2 1 CABI, Bakeham Lane, Egham, Surrey TW20 9TY, UK 2 CABI, Rue des Grillons 1, CH-2800 Delémont, Switzerland; [email protected] * Correspondence: [email protected] Simple Summary: Recent globalization of trade and travel has led to the introduction of exotic insects into Europe, including the brown marmorated stink bug (Halyomorpha halys)—a highly polyphagous pest with more than 200 host plants, including many agricultural crops. Unfortunately, farmers and crop-protection advisers finding egg masses of stink bugs during crop scouting frequently struggle to identify correctly the species based on their egg masses, and easily confuse eggs of the invasive H. halys with those of other (native) species. To this end, we have investigated using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid, fieldwork-compatible, and low-reagent-cost discrimination between the eggs of native and exotic stink bugs. Abstract: In the current paper, we used a method based on stink bug egg-protein immobilization on filter paper by drying, followed by post-(storage and shipping) extraction in acidified acetonitrile containing matrix, to discriminate between nine different species using MALDI-TOF MS. We obtained 87 correct species-identifications in 87 blind tests using this method. With further processing of the unblinded data, the highest average Bruker score for each tested species was that of the cognate Citation: Reeve, M.A.; Haye, T. reference species, and the observed differences in average Bruker scores were generally large and the Discrimination between Eggs from errors small except for Capocoris fuscispinus, Dolycoris baccarum, and Graphosoma italicum, where the Stink Bugs Species in Europe Using average scores were lower and the errors higher relative to the remaining comparisons.
    [Show full text]
  • Brown Marmorated Stink Bug, Halyomorpha Halys
    Sparks et al. BMC Genomics (2020) 21:227 https://doi.org/10.1186/s12864-020-6510-7 RESEARCH ARTICLE Open Access Brown marmorated stink bug, Halyomorpha halys (Stål), genome: putative underpinnings of polyphagy, insecticide resistance potential and biology of a top worldwide pest Michael E. Sparks1* , Raman Bansal2, Joshua B. Benoit3, Michael B. Blackburn1, Hsu Chao4, Mengyao Chen5, Sammy Cheng6, Christopher Childers7, Huyen Dinh4, Harsha Vardhan Doddapaneni4, Shannon Dugan4, Elena N. Elpidina8, David W. Farrow3, Markus Friedrich9, Richard A. Gibbs4, Brantley Hall10, Yi Han4, Richard W. Hardy11, Christopher J. Holmes3, Daniel S. T. Hughes4, Panagiotis Ioannidis12,13, Alys M. Cheatle Jarvela5, J. Spencer Johnston14, Jeffery W. Jones9, Brent A. Kronmiller15, Faith Kung5, Sandra L. Lee4, Alexander G. Martynov16, Patrick Masterson17, Florian Maumus18, Monica Munoz-Torres19, Shwetha C. Murali4, Terence D. Murphy17, Donna M. Muzny4, David R. Nelson20, Brenda Oppert21, Kristen A. Panfilio22,23, Débora Pires Paula24, Leslie Pick5, Monica F. Poelchau7, Jiaxin Qu4, Katie Reding5, Joshua H. Rhoades1, Adelaide Rhodes25, Stephen Richards4,26, Rose Richter6, Hugh M. Robertson27, Andrew J. Rosendale3, Zhijian Jake Tu10, Arun S. Velamuri1, Robert M. Waterhouse28, Matthew T. Weirauch29,30, Jackson T. Wells15, John H. Werren6, Kim C. Worley4, Evgeny M. Zdobnov12 and Dawn E. Gundersen-Rindal1* Abstract Background: Halyomorpha halys (Stål), the brown marmorated stink bug, is a highly invasive insect species due in part to its exceptionally high levels of polyphagy. This species is also a nuisance due to overwintering in human- made structures. It has caused significant agricultural losses in recent years along the Atlantic seaboard of North America and in continental Europe.
    [Show full text]
  • Brown Marmorated Stink Bug, Halyomorpha Halys (Stål) (Insecta: Hemiptera: Pentatomidae)1 Cory Penca and Amanda Hodges2
    EENY346 Brown Marmorated Stink Bug, Halyomorpha halys (Stål) (Insecta: Hemiptera: Pentatomidae)1 Cory Penca and Amanda Hodges2 Introduction The brown marmorated stink bug (BMSB), Halyomorpha halys (Stål) (Figure 1), is an invasive stink bug first identi- fied in the United States near Allentown, Pennsylvania, in 2001, though it was likely present in the area several years prior to its discovery (Hoebeke and Carter 2003). In the United States, the brown marmorated stink bug has emerged as a major pest of tree fruits and vegetables, caus- ing millions of dollars’ worth of crop damage and control costs each year (Leskey et al. 2012a). The brown marmo- rated stink bug has also become a nuisance to homeowners due to its use of structures as overwintering sites (Inkley Figure 1. An adult brown marmorated stink bug, Halyomorpha halys 2012). The significance of the brown marmorated stink bug (Stål). Credits: Lyle J. Buss, UF/IFAS has resulted in a large body of academic, government, and private sector research focused on its control. Distribution The brown marmorated stink bug is native to eastern Synonymy Asia, including China, Taiwan, Korea, and Japan (Lee et Pentatoma halys Stål (1855) al. 2013). Since its discovery in North America the brown marmorated stink bug has spread rapidly throughout the Poecilometis mistus Uhler (1860) eastern and midwestern United States, as well as establish- ing on the West Coast (Figure 2). Following its invasion Dalpada brevis Walker (1867) of North America, the brown marmorated stink bug has expanded its global range into Europe, Eurasia, and South Dalpada remota Walker (1867) America (Chile), making it an invasive species with a global impact (Wermelinger et al.
    [Show full text]
  • Invasive Stink Bugs and Related Species (Pentatomoidea) Biology, Higher Systematics, Semiochemistry, and Management
    Invasive Stink Bugs and Related Species (Pentatomoidea) Biology, Higher Systematics, Semiochemistry, and Management Edited by J. E. McPherson Front Cover photographs, clockwise from the top left: Adult of Piezodorus guildinii (Westwood), Photograph by Ted C. MacRae; Adult of Murgantia histrionica (Hahn), Photograph by C. Scott Bundy; Adult of Halyomorpha halys (Stål), Photograph by George C. Hamilton; Adult of Bagrada hilaris (Burmeister), Photograph by C. Scott Bundy; Adult of Megacopta cribraria (F.), Photograph by J. E. Eger; Mating pair of Nezara viridula (L.), Photograph by Jesus F. Esquivel. Used with permission. All rights reserved. CRC Press Taylor & Francis Group 6000 Broken Sound Parkway NW, Suite 300 Boca Raton, FL 33487-2742 © 2018 by Taylor & Francis Group, LLC CRC Press is an imprint of Taylor & Francis Group, an Informa business No claim to original U.S. Government works Printed on acid-free paper International Standard Book Number-13: 978-1-4987-1508-9 (Hardback) This book contains information obtained from authentic and highly regarded sources. Reasonable efforts have been made to publish reliable data and information, but the author and publisher cannot assume responsibility for the validity of all materi- als or the consequences of their use. The authors and publishers have attempted to trace the copyright holders of all material reproduced in this publication and apologize to copyright holders if permission to publish in this form has not been obtained. If any copyright material has not been acknowledged please write and let us know so we may rectify in any future reprint. Except as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced, transmitted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter invented, including photocopying, micro- filming, and recording, or in any information storage or retrieval system, without written permission from the publishers.
    [Show full text]