“WE SHARE OUR SCIENCE TO FEED THE FUTURE”

WWW.IRTA.ES

“Research and transfer in aquaculture at IRTA” ______

A public company of the Government of Catalonia, linked to the Department of Agriculture, Food and Rural Action (DAR), subject to private law.

“To contribute to the modernization, competitiveness and sustainable development of agriculture, food and aquaculture sectors, the supply of healthy foods and quality for consumers, improving the welfare of the population.” ______The Centres Llesp

Mas Badia Field Station Semega Monells Centa Gimenells Alcarràs Field Station Torre Marimon Fruit Centre Lleida Field Station Lleida Borges Blanques Cabrils CReSA Mollerussa CRAG CREAF UdL-IRTA Foundation Corporate Services Valls CREDA Mas de Bover Ascó Gandesa

Ebre Field Station IRTA work centres Sant Carles de la Ràpita Associated centres IRTA field stations Associated field stations Collaborating farms ______IRTA Sant Carles de la Ràpita In the heart of the Ebro Delta

Multifunctional platform in aquaculture and aquatic ecosystems ______RESEARCH ACTIVITIES END USERS Marine Environmental Survey • producers • Food safety in aquaculture products. • Consumers and retailers • Studies on harmful microalgae (population dynamics, , cultures, toxinic production) • Human health agencies • Isolation of toxins and substances that are biological active (chromatography, biosensors, cytotoxicity) • Contaminants • Coastal Oceanography.

Aquatic cultures • Consumers and retailers • Genetics and Reproduction • Feed and additive producers • Larval culture and Nutrition • Fish and shellfish farmers • Aquatic Health • Aquatic health agencies and companies • Conservationists bodies Aquatic Ecosystems • Floods and pulses of water, sediments and nutrients. • Citizens • Structure and dynamics of habitats and populations. • Environmental agencies • Biogeochemical cycles. • Conservationists bodies • Anthropogenic impacts ______Facilities ______Research IRTAmar® quality standard conditions AQUACULTURE PROGRAM Target ______

Sparus aurata Dicentrarchus labrax Solea senegalensis Argyrosomus regius Thunnus thynnus

Salmo salar Orcorhynchus mykiss Oreochromis Anguilla anguilla Acipenser niloticus oxyrinchus oxyrinchus

Reproduction Larval rearing Nutrition Stock enhancement Fish reproduction ______

Research Topics of the Area

• Chemical communication and spawning behaviour • Endocrinology and control of fish reproduction • Broodstock nutrition • Genetic selection of cultured fish • Cell biology of fish gametes • Preservation of fish gametes and embryos • Development of genomics and proteomics tools • Genomics of desiccation resistance in fish embryos Nutritional requirements and impact of nutrition on larval quality ______

Assessment of the effects of dietary components on larval performance and establishment of their optimal nutritional requirements.

Macronutrients: Feed additives: Micronutrients: immunestimulants, lipid classes, HUFAs, vitamin A, other vitamins, probiotics protein hydrolysates minerals

Live prey Microdiets (enriched rotifers & Artemia)

Nutritional dose-response trials

Organism Cell & Tissue Gene Growth Skeletogenesis PPARs Survival Digestive function RARs – RXRs Phenotype Immune function BMP, IGF, etc Pigmentation ______

Effect of alternative diets on juvenile and larval fish performance.

The need for more sustainable diets  considerable changes in diet formulation but effects in important production parameters have yet not been assessed.

Fish oil replacement by vegetable oils in aquaculture diets  changes in fatty acid profiles (such as in n-6 /n-3 PUFA ratios)  physiological and metabolic changes  effects on food intake? Mollusc culture ______

Hatchery & Nursery pilot culture Mollusc culture ______

SPECIES Pacific ( gigas) Flat oyster () Variegated (Chlamys varia) (Octopus vulgaris) Ribbed Mediterranean ( ferruginea)

OBJECTIVES Close the biological cycle Improvements in growth, fattening and disease resistance. Stock enhancement

SECTOR Generalitat de Cataluña- DAR (Regional government) Shellfish producers Spanish Ministry of Agriculture, Fisheries and Food National Research Council (CSIC) Mollusc culture ______

# General objective AQUATIC HEALTH To develop an integrated pathology area focused on animal health problems and public health related to aquaculture.

UCE USM Handling culture Animal Health Sanitary control. species Environmental Human Health phenomena

Pathology Group UEA Ecology and biodiversity

Horizontal Activities Research integrated objectives Mollusc health ______

Epidemiology and Diseases

• Epidemiological approaches and risk based assessment. • Classic and molecular techniques for diagnostic. • Parasites lifecycle. Marteilia sp. in , and cockles

Rickettsia-like in smooth

Perkinsus sp. in Bonamia sp. in oysters Aggregata sp. in Octopus ______

• 500 m2 building • Air-conditioned • Sea water • Fresh Water • Brackish water • Heating & cooling systems • 6 Recirculation aquaculture systems 1. Development and application of diagnostic methods • Ozone treatment of waste water 2. Epidemiology and risk analysis 3. Analysis of gene expression related pathologies 4. Challenge room: a. Experimental infections b. Study the parasite lifecycle c. Testing: immune additives, probiotics. vaccines, drugs. 5. Fish welfare The Marine Monitoring and Food Safety ______Ciguatoxin-like toxicity detection in First domoic acid detection in clams The Marine fish from the Canary Islands from the Catalan coast Monitoring (Seriola spp.) by cell-based assays program guarantees safe shellfish, protects human health, supports the Homoyessotoxin standard production * (A) aquaculture production * * * * sector in * * * Discovery of acyl* ester metabolites of * Catalonia and gymnodimines in shellfish using LC-MS preserves the * (B) marine * environment. * Magnetic particles as antibody and enzyme * * * * * * * supports for biosensor development

Molecular probes for Pseudo-nitzschia spp. Work plan Descriptive data: Environment Oceanography Phytoplankton Population Dynamics Field …

Samples Food Water Microalgae

Culture

Laboratory Microbiology Chemical pollutants analysis & experiments Toxins

Chemistry Biochemistry Cell bioassays Exposure of marine organisms Structure Functional description of toxins Risk assessment Mechanisms of action Physiology Development of Toxicology biosensors … Bioactive compounds Potential integration ______

Customer

Production and design Experimental Platform in of experimental feeds nutritional trials IRTA Mas Bové IRTA Sant Carles de la Ràpita

Food technology and safety Genetics IRTA Monells IRTA Monells Thank you ______