Course Course Volume in Course hours code group ECTS credits

BIO 4016 C 6 160

Course type (compulsory or optional) Compulsory Course level (study cycle) Bachelor Semester the course is delivered Spring Study form (face-to-face or distant) Face-to-face Course title in Lithuanian MOLEKULINĖ GENETIKA Course title in English MOLECULAR GENETICS Short course annotation in Lithuanian Molekulinės genetikos principai ir pagrindinės sąvokos. Genų molekulinė struktūra ir funkcijos, ekspresijos ir reguliavimo m molekulinis mechanizmas. Molekuliniai genų ir genomo tyrimų metodai. Tiesioginė ir atvirkštinė genetika. Bakterijų, mielių, augalų, žmogaus molekulinė genetika. Genų terapija. Molekulinės genetikos panaudojimas diagnostikoje. Vėžio genetika Short course annotation in English This program encompasses studies the structure and function of genes, inheritence, expression and regulation at a molecular level. Students will be introduced to useful tools and concepts that enable the study of a wide spectrum of molecular genetics; sample collection; genome scanning with subsequent linkage analysis; mutation detection methods; and the use of animal methods. All of the favorite model organisms used in modern genetic analysis are represented in this program, including phage and viruses, bacteria, yeast and fungi, nematodes, Drosophila, algae, plants, and mice. This program is rounded out by an additional strength in human molecular genetics, a research area of increasing importance. Prerequisites for entering the course Basics biology, Biochemistry, General genetics. Course aim The aim of the course is to introduce principles and concepts of molecular genetics and to provide basic knowledge of molecular genetic methods and its applications. Links between course outcomes and criteria of learning achievement evaluation Course outcomes Criteria of learning achievement evaluation presents principles of gene and chromosomes and Understand the principles of microbial, plant and genetic manipulation, main problems of genomic and animal molecular genetics human projects, presents methods of microbial, plant and animal cell and Acquire skills in specialized methods of molecular tissue culture, methods of genetic manipulation, DNA and genetic engineering amplification, separation and detection,. Acquire knowledge about principles of gene presents application of molecular methods in genom manipulation and ethical questions of their use. analysis, ethical issues of application of human genom. Content (topics) 1. Principles and concepts of molecular genetics 2. Gene structure and genom. 3. Reverse genetics 4. Forward genetics. 5. Techniques in molecular genetics: DNA isolation, mRNA isolation, amplification, PGR, separation and detection. and expression 6. Molecular Genetics of Bacteria 7. Yeast Molecular Genetics 8. Plant Molecular Genetics 9. The Human Genome Project 10. Gene therapy 11. Cancer gGenetics 12. Epigenetic Practical work (contents): eucaryotic cell and tissue culture; plasmid DNA preparation; DNA amplification and analysis; genetic transofrmation; and analysis techniques. Distribution of workload for students (contact and independent work hours) Lectures – 30 hours, laboratory work – 20 hours, individual work – 58 hours. Structure of cumulative score and value of its constituent parts Final assessment sums the assessments of written final examination (50%), written mid-term examination (20%) and assessment of laboratory works (30%). Recommended reference materials Number of copies in Authors of No. Publication Self- publication Publishing house University Other year study and title library libraries rooms Basic materials Lewin‘s GENES edit. J.E.Krebs, Jones and Bartlett publishers 1. 2011 D.T.Kilpatrick, 1 1 E.S. Goldstein. J. D. Watson, T.A. Baker, S.P. Benjamin-Cummings Publishing 2. 2007 Bell. Molecular 1 1 Company Biology of the Gene T Strachan, A.P. Read. Human 3. 2010 Garland Publishing 1 1 Molecular Genetics Sasnauskas K. 4. 2006 Molekulinė Garland Publishing 1 1 biologija Supplementary materials Molecular Genetics http://link.springer.com/journa 1. 2000-2012 and Genomics l/438 Human molecular 2. 2000-2012 http://hmg.oxfordjournals.org/ genetics L. Snyder, W. ASM Press Champness. 3. 2003 Molecular Genetics of Bacteria Seiler J.P., Good 6. 2005 laboratory practice, Springer 2nd edition Course programme designed by Prof.Dr. Algimantas Paulauskas, Department of Biology, Faculty of Natural Sciences