One cell biology Our cell biology portfolio at a glance
We have simplified our portfolio to show you how our most popular products and services are used whether you are looking for a single tool or more comprehensive and integrated solutions.
This handbook is meant to expedite your search for the right cell biology product and give you an in-depth look at the many ways we can help accelerate research.
Complete products Products for cell, protein, and Media, FBS, supplements, for bioproduction, cell molecular biology technologies, cells, and reagents for culture, molecular biology, and transfection and cell culture protein biology, and drug discovery high-content analysis Contents
Cell biology: harnessing the power of the cell 4
Where our products work 5
Most popular products 6
Products used in research 8
Product categories and offerings 16
Additional resources 18 Cell biology: harnessing the power of the cell
Cell biology is the study of the structure and functions of cells. We offer the tools necessary to investigate changes to these fundamental building blocks of life. We provide a comprehensive selection of products of superior quality, designed to help researchers achieve their next breakthrough. We enable our customers to harness the power of cells to improve human health.
1 Source
2 Culture
3 Modify
4 Analyze
4 Where our products work
Our products help you culture, modify, and analyze cells. Or, we can do it for you with one of our many customized services. Workflow product categories Starting with the right cell type or cell line is the foundation for • Primary cells • Engineered cell lines Source any experiment. 1 • Stem cells • Reprogramming
Cell culture is the process by which cells are grown in a controlled, • Media and supplements • Cell culture plastics Culture artificial environment. 2 • FBS and other sera • Matrices
Essential tools for transfection, protein expression, genome editing, • Gene synthesis • Gene editing Modify and cell differentiation form the basis for many research projects. 3 • Transfection and protein expression • Cell differentiation
There are a variety of tools that help researchers analyze cells to • Characterization • Screening Analyze better understand, predict, and influence the underlying factors of 4 cell health, proliferation, function, and death. • Counting and imaging
We offer a wide range of services for cell-based research to help • Stem cell services • Custom biology services Services scientists at any point in their workflow. 5 • Drug discovery services
5 Most popular products
These are some of the most popular products from our portfolio. The presence of these products in the following categories illustrates where our newest or best-known products can help you get results.
3D models, organoids, and spheroids Plastics Neurobiology • Optimized plastics, media, supplements, matrices, • Thermo Scientific™ Nunc™ EasYFlask™ and EasYDish™ • Gibco™ B-27™ Plus Supplement reagents, primary cell lines, growth factors, and more cultureware thermofisher.com/b27
™ ™ thermofisher.com/organoid • Thermo Scientific ™ ™ Nunc cell culture plates • Gibco CultureOne Supplement • Thermo Scientific™ Nunclon™ Sphera™ U-bottom 96-well Cell culture thermofisher.com/cultureone plates ™ ™ • Gibco™ Dulbecco's Modified Eagle Medium (DMEM) • Gibco Neurobasal Plus Medium • Thermo Scientific™ Nunc™ conical tubes • Gibco™ RPMI 1640 Medium thermofisher.com/neurobasal thermofisher.com/cellcultureplastics thermofisher.com/media Protein expression ™ • Gibco Pluripotent stem cells ™ ™ Hank's Balanced Salt Solution (HBSS) • Gibco ExpiCHO Expression System • Invitrogen™ CytoTune™-iPS 2.0 Sendai thermofisher.com/balancedsalts thermofisher.com/expicho ™ ™ Reprogramming Kit • Gibco ™ ™ TrypLE Express Enzyme • Gibco Expi293 Expression System ™ thermofisher.com/cytotune • Gibco Trypsin solutions thermofisher.com/expi293 • Gibco™ StemFlex™ Medium thermofisher.com/celldissociation ™ ™ • Gibco ExpiSf Expression System thermofisher.com/stemflex thermofisher.com/expisf Sera • Gibco™ Essential 8™ Medium • Gibco™ Value FBS* thermofisher.com/essential8 Transfection ™ • Gibco ™ ™ ™ ™ Premium FBS* • Gibco KnockOut Serum Replacement • Invitrogen Lipofectamine 3000 Transfection Reagent ™ • Gibco Specialty FBS* thermofisher.com/ksr thermofisher.com/lipofectamine3000 thermofisher.com/fbs ™ ™ • Invitrogen Neon Transfection System Cell therapy thermofisher.com/neon Matrices • Gibco™ CTS™ AIM V™ SFM** ™ ™ ™ • Invitrogen Lipofectamine Stem Transfection Reagent • Gibco Collagen • Gibco™ CTS™ OpTmizer™ T Cell Expansion SFM† ™ ™ thermofisher.com/lipofectaminestem • Gibco AlgiMatrix 3D Culture System • Gibco™ CTS™ StemPro™ MSC SFM† ™ ™ ™ • Invitrogen Lipofectamine RNAiMAX • Gibco rhLaminin-521 • Gibco™ CTS™ Immune Cell Serum Replacement (SR)‡ ™ ™ Transfection Reagent • Gibco Geltrex matrices • Gibco™ CTS™ Essential 8™ Medium‡ ™ thermofisher.com/rnaimax • Gibco Poly-D-Lysine • Gibco™ CTS™ CytoTune™-iPS 2.1 Sendai • Gibco™ LV-MAX ™ Transfection Kit thermofisher.com/3dcellculture Reprogramming Kit§ thermofisher.com/lvmax thermofisher.com/cts
* Also available in the innovative Gibco™ ** For Human Ex Vivo Tissue and Cell Culture † For Human Ex Vivo Tissue and Cell Culture ‡ For Research Use or Manufacturing of Cell, § For Research Use or Non-Commerical One Shot™ 50 mL bottles and in heat- Processing Applications. Caution: Not intended Processing Applications. Caution: When used Gene, or Tissue-Based Products. Caution: Not Manufacturing of Cell-Based Products for inactivated format. for direct administration to humans or animals. as a medical device, federal law restricts this intended for direct administration into humans Clinical Research. Caution: Not intended for 6 device to sale by or on the order of a physician. or animals. direct administration into humans or animals. 7 Products used in research
1 Source 2 Culture
Area of research Cell type or cell line Reprogramming Media Plastics Supplements Matrices Dissociation
• Gibco™ FGF2, EGF, BDNF, NT-3, DKK1, BMPR1A, GDNF, Laminin, and bFGF • Gibco™ DMEM/F-12 recombinant proteins ™ ™ • Gibco™ RevitaCell™ • Gibco Neurobasal ™ ™ Medium Supplement • Gibco Geltrex matrix ™ ™ Brain • Pluripotent stem cell (PSC) ™ • Gibco™ Essential 8™ Medium • Gibco N-2 Supplement • Gibco AlgiMatrix 3D Culture System • Gibco™ Essential 6™ Medium • Gibco™ B-27™ Supplement • Gibco™ StemFlex™ Medium • Gibco™ GlutaMAX™ Supplement • Gibco™ KnockOut™ Serum Replacement (SR)
• Gibco™ HepaRG™ Medium • Gibco™ HepExtend™ Media Kit • Gibco™ Primary Hepatocyte • Primary hepatocyte • Gibco™ Advanced Maintenance Supplements DMEM/F12 • GlutaMAX Supplement 3D organoids • Gibco™ Williams’ E Medium, and spheroids no phenol red • Thermo Scientific™ Nunclon™ • Geltrex matrix Liver Sphera™ plates, flasks, and • Gibco™ FGF10, HGF, EGF, • AlgiMatrix 3D • Adult tissue • Gibco Advanced • Specific products are dishes Noggin, BMP7, and FGF19 Culture System • Stem or progenitor cell available for PSC lines; find DMEM/F-12 ™ ™ • Thermo Scientific™ recombinant proteins • Gibco TrypLE out more at thermofisher. Nalgene™ Single-Use PETG Express Enzyme com/organoid or reach • Gibco™ HGF, EGF, BMP4, Erlenmeyer Flasks • Gibco™ Versene Solution out to a technical sales • Gibco DMEM/F-12 VEGF, Activin, PDGFBB, • Thermo Scientific™ Nunc™ • PSC specialist • Gibco™ RPMI 1640 Medium and FGF2 recombinant Polycarbonate Cell Culture ™ ™ proteins Find out more at • Gibco StemPro MSC SFM* Inserts thermofisher. • KnockOut SR com/organoid • Gibco Advanced DMEM/F-12 • Gibco™ Activin, FGF4, BMP2, EGF, Noggin, • PSC • Gibco DMEM/F-12 SHH, IGF1, and FGF2 ™ ™ • Geltrex matrix • Gibco KnockOut DMEM recombinant proteins Intestine • Gibco RPMI 1640 Medium • AlgiMatrix 3D Culture System • Gibco DMEM • Adult tissue • Gibco™ Noggin, EGF, and • Gibco Advanced • Stem or progenitor cell FGF10 recombinant proteins DMEM/F-12
• Gibco™ FGF2, Noggin, • Gibco™ Advanced RPMI Activin, FGF9, BMP4, • Geltrex matrix Kidney • PSC 1640 Medium BMP7, and LIF recombinant • AlgiMatrix 3D • Gibco DMEM/F-12 proteins Culture System • KnockOut SR
• Gibco Advanced • Activin, FGF4, FGF2, • Geltrex matrix Lung • PSC DMEM/F-12 Noggin, and SHH • AlgiMatrix 3D • Gibco RPMI 1640 Medium • KnockOut SR Culture System
* For Human Ex Vivo Tissue and Cell Culture Processing Applications. Caution: When used as a medical device, federal law restricts this device to sale by or on the order of a physician.
8 3 Modify 4 Analyze 5 Services
Area of research Gene editing Gene delivery Differentiation Characterization Screening or imaging Services
Brain
3D organoids ™ Custom biology services: and spheroids • Applied Biosystems ™ QuantStudio real-time • Thermo Scientific™ CellInsight™ • Cell line engineering Liver PCR instruments CX7 LZR High-Content Analysis • Cas9 iPSC lines • Invitrogen™ CRISPR products • Applied Biosystems™ TaqMan ® (HCA) Platform • Custom media solutions and services hPSC Scorecard Panel • Thermo Scientific™ Varioskan™ N/A* N/A* • Invitrogen™ TALEN products and • Applied Biosystems™ LUX Multimode Microplate Cell model services: TaqMan ® Assays Reader services • Applied Biosystems™ KaryoStat™ ™ ™ ™ Find out • Invitrogen reagents • Invitrogen EVOS characterization services ™ imaging systems more at • Invitrogen functional assays • PluriTest™ pluripotency service thermofisher. and readouts com/organoid • TaqMan hPSC Scorecard service
Intestine
Kidney
Lung
* These tools can be used prior to 3D culturing in a general cell culture research step. Contact your local technical specialist to learn more.
9 Products used in research continued
1 Source 2 Culture
Area of research Cell type or cell line Reprogramming Media Plastics Supplements Matrices Dissociation
• Gibco™ Geltrex™ LDEV- Free Reduced Growth
• Induced PSC (iPSC)/ ™ Factor Basement • Invitrogen™ CytoTune- • Essential 8 Medium • Thermo Scientific human embryonic stem ™ • RevitaCell Supplement Membrane Matrix iPS™ 2.0 Sendai • StemFlex Medium Nunclon Delta flasks, cell (hESC) products • Gibco™ Vitronectin Reprogramming Kit dishes, and plates • KnockOut SR • Gibco DMEM/F-12 truncated protein • Episomal iPSC line • Thermo Scientific™ (VTN-N) Nunc™ poly-D-lysine– ™ • Versene Solution coated flasks and plates • GIbco rhLaminin-521 • StemPro medium • Thermo Scientific™ • Geltrex LDEV-Free ™ ™ Nunc™ Lab-Tek II • Gibco StemPro • Human NSCs • Neurobasal Plus • B-27 Plus Supplement Reduced Growth ™ chamber slides with Accutase Cell Neurobiology • Gibco™ Human Medium • CultureOne Supplement Factor Basement CC2 glass Dissociation Reagent Astrocytes (primary cell) • Gibco DMEM/F-12 Membrane Matrix ™ ™ ™ • KnockOut SR • Gibco TrypLE Select • Thermo Scientific • Gibco rhLaminin-521 Nunc™ optical bottom Enzymes N/A • Neurobasal Medium plates with polymer or 2 • Neurobasal Plus CC glass base • Primary neurons Medium • Nunclon Sphera flasks, • B-27 Plus Supplement dishes, and plates • Poly-D-Lysine Find out more at (mouse and rat) • Premium FBS, Certified • CultureOne Supplement thermofisher.com/neuro • Specialty FBS, charcoal stripped or dialyzed • Invitrogen™ Episomal • Geltrex LDEV-Free iPSC vectors • Essential 8 and • KnockOut SR Reduced Growth Factor • Versene Solution • Invitrogen™ Epi5™ Essential 8 Flex Media • Gibco™ Knockout™ SR • iPSC/ESC products Basement Membrane • StemPro Accutase Cell Episomal kit • StemFlex Medium Multi-Species (mouse • Episomal iPSC line Matrix Dissociation Reagent • CytoTune-iPS 2.0 kit • Gibco™ KnockOut™ and human) • Gibco VTN-N • T rypLE Select Enzyme ™ ™ DMEM/F-12 • RevitaCell Supplement • Gibco CTS CytoTune- • Gibco rhLaminin-521 iPS 2.1 kit** • Nunclon Delta flasks, • Gibco™ MesenPRO RS™ dishes, and plates Stem cells Medium† • Nunclon Sphera plates, • StemPro MSC SFM‡ flasks, and dishes • Mesenchymal stem cells • Gibco™ CTS™ CELLstart™ N/A • Nunc Lab-Tek and Lab- • GlutaMAX Supplement • TrypLE Select Enzyme (MSCs)* • StemPro MSC SFM, substrate** XenoFree‡ Tek II chamber slides • Specialty FBS, MSC • Nunc optical Qualified bottom plates • GIbco™ StemPro™ Neural • Geltrex LDEV-Free Supplement‡ Reduced Growth Factor • StemPro Accutase Cell • KnockOut DMEM/F-12 • B-27 Supplement, Basement Membrane Dissociation Reagent • NSCs N/A Find out more at • StemPro NSC SFM minus vitamin A Matrix • TrypLE Express thermofisher.com/ • N-2 Supplement • Gibco VTN-N Enzyme stemcells • GlutaMAX Supplement • Gibco rhLaminin-521
* We do not offer this product. ** For Research Use or Non- † For Research Use or Further ‡ For Human Ex Vivo Tissue Commerical Manufacturing of Manufacturing. Not for diagnostic and Cell Culture Processing Cell-Based Products for Clinical use or direct administration in to Applications. Caution: When Research. Caution: Not intended humans or animals. used as a medical device, federal 10 for direct administration into law restricts this device to sale humans or animals. by or on the order of a physician. 3 Modify 4 Analyze 5 Services
Area of research Gene editing Gene delivery Differentiation Characterization Screening or imaging Services
• Gibco™ PSC Dopaminergic • Invitrogen™ Alkaline Phosphatase Neuron Differentiation Kit (AP) Live Stain • Gibco™ PSC Neural Induction • EVOS imaging systems • Invitrogen™ Lipofectamine™ Stem Medium • CellInsight HCA/HCS platforms Transfection Reagent • TaqMan hPSC Scorecard Panel • Invitrogen™ Lipofectamine™ and Analysis RNAiMAX Transfection Reagent* ™ ™ ™ ™ • Applied Biosystems PrimeView • Invitrogen Neon Transfection • B-27 Plus Supplement System Global Gene Expression Profile • CultureOne Supplement • CRIPSR products and services Assays • Gibco™ CellModel™ Differentiation Neurobiology • Invitrogen™ outgrowth assay • TALEN products and services • Applied Biosystems™ KaryoStat Services and KaryoStat HD Assays
• Invitrogen™ Lipofectamine™ MessengerMAX™ Transfection • Invitrogen™ reagents Reagent • Invitrogen™ NeuroTrace™ stains • Invitrogen™ Lipofectamine™ 2000 N/A ™ Transfection Reagent • Invitrogen Neurite Outgrowth Staining Kit • Lipofectamine RNAiMAX Transfection Reagent*
Neural • Invitrogen AP • PSC Neural Induction Medium • EVOS imaging systems • PSC Dopaminergic Neuron Differentiation Kit • CellInsight HCA/HCS platforms Cardiac • TaqMan hPSC Scorecard Panel CellModel services and Analysis • Gibco™ PSC Cardiomyocyte • Reprogramming • Lipofectamine Stem • PrimeView Global Gene Differentiation Kit • Assay development Transfection Reagent Expression Profile Assays Endoderm • Applied Biosystems™ MycoSEQ™ • Differentiation • Lipofectamine RNAiMAX • KaryoStat and KaryoStat ™ Mycoplasma Detection Assay Transfection Reagent* • Gibco PSC Definitive Endoderm HD Assays • Cell screening Induction Kit • Neon Transfection System • Characterization • Gibco™ StemPro™ Adipogenesis • Custom media • CRIPSR products and services ™ ™ (GMP and cGMP options) Stem cells Differentiation Kit • Invitrogen Attune NxT Flow • TALEN products and services • Gibco™ StemPro™ Chondrogenesis Cytometer Differentiation Kit • Applied Biosystems™ TaqMan ® • Gibco™ StemPro™ Osteogenesis Gene Expression Assays Differentiation Kit
• EVOS imaging systems • CellInsight HCA/HCS platforms • Lipofectamine Stem • TaqMan hPSC Scorecard Panel • B-27 Plus Supplement • Biochemical and cellular assays Transfection Reagent and Analysis • Neurobasal Plus Medium • MycoSEQ Mycoplasma • CellModel Differentiation Services • Lipofectamine RNAiMAX • PrimeView Global Gene • CultureOne Supplement Detection Assay Transfection Reagent* Expression Profile Assays • KaryoStat and KaryoStat HD Assays
* For knockdown studies across all areas of research. 11 Products used in research continued
1 Source 2 Culture
Area of research Cell type or cell line Media Plastics Supplements Matrices Dissociation
• Gibco Williams’ E Medium • Gibco™ MEM • Immortalized hepatocytes • Gibco DMEM • GlutaMAX Supplement, • Collagen (HepG2*, HepaRG) • Gibco™ Synth-a-Freeze™ Cryopreservation Medium Metabolism, induction/tox • Geltrex matrix • Premium FBS, Certified supplement for HepaRG • GlutaMAX Supplement • Nunclon Delta flasks, dishes, ™ and plates • Gibco Hepatocyte Thaw Medium • Collagen • TrypLE Express enzymes Drug development • Premium FBS, Certified • Nunc ECM-coated flasks • Hepatocytes (primary) • Gibco™ Primary Hepatocyte • Laminin • Trypsin solutions • Gibco Williams’ E Medium and plates (ADME/Tox and DMPK) Maintenance Supplement • Nunc optical bottom plates • Geltrex matrix • Gibco™ HepExtend™ Supplement Find out more at • Gibco DMEM, Gibco Williams’ E Medium • Liver nonparenchymal cells • GlutaMAX Supplement • Collagen thermofisher.com/admetox (Kupffer, Stellate, and LSEC* • RPMI 1640, FBS, and exosome-depleted FBS • HepExtend Supplement • Geltrex matrix cells) • Synth-a-Freeze Cryopreservation Medium • Invitrogen™ EpiLife™ Medium • Gibco™ Medium 154, 254, 106, 131 • Growth factors: (HKGS, • Skin keratinocytes, • Gibco DMEM/F-12 EDGS, HMGS, LSGS, melanocytes, and dermal • Premium FBS, Certified MVGS) (EpiLife, LSGS) fibroblasts • Nunclon Delta flasks, dishes, • Gibco VTN-N • Gibco™ Keratinocyte-SFM and Defined and plates • Synth-a-Freeze medium Primary cells • Gibco rhLaminin Keratinocyte-SFM • Nunc Lab-Tek and Lab-Tek II • TrypLE Select enzymes • Gibco™ attachment factors ™ ™ chamber slides • Gibco Trypsin-EDTA • Heart endothelial cells • Gibco Medium 200, 231 • LVES, SMGS, and SMDS • TrypLE enzymes • Nunc optical bottom plates solutions ™ • Coating Matrix Kit Find out more at • Gibco™ Medium 171 • Nunc ECM-coated flasks • Gibco HuMEC • Mammary epithelial (HMEC) Supplement Kit • Gibco Poly-D-Lysine thermofisher.com/primarycells • Gibco™ HuMEC Ready Medium and plates cells ™ ™ • Gibco Mammary Epithelial • Gibco HuMEC Basal Serum-Free Medium Growth Supplement (MEGS) • Skeletal myoblast cells • Gibco™ DMEM, low glucose, pryuvate • 2% horse cells
Biologics • Gibco™ ExpiCHO-S™ Cells • Gibco™ ExpiCHO™ Expression Medium
Protein expression • Invitrogen™ Flp-In™ T-Rex™ 293 Structural Cell Line • Gibco™ FreeStyle™ 293 Expression Medium • Nalgene Single-Use PETG • GlutaMAX Supplement biology ™ N/A N/A ™ ™ Erlenmeyer Flasks • Gibco L-Glutamine** Find out more at • Gibco Expi293F Cells
thermofisher.com/ ™ ™ ™ Intracellular • Gibco Sf9, S-21, and • Gibco Sf-900 II and III SFM proteinexpression ™ protein High Five cells • Gibco™Express Five™ SFM • MCF-7*, MDA-MB-231*, Breast cancer • Nunclon Delta–treated and T-47D*, and BT-474* cells • Gibco DMEM, (high glucose) non-treated flasks, dishes, • Specialty FBS, charcoal stripped Liver cancer • HepG2* and Hep3B* cells and plates • Nunclon Sphera flasks, • Gibco™ Penicillin- • TrypLE Express enzymes • A549*, NCI-H975*, PC-14*, Lung cancer • Gibco RPMI 1640 Medium dishes, and plates Streptomycin • Gibco Collagen I Cancer/therapeutic and NCI-H1299* cells • One Shot FBS, Certified • Nunc ECM-coated flasks • GlutaMAX Supplement • Gibco Fibronectin Prostate • DU145*, PC-3*, and • Gibco DMEM and plates • L-Glutamine** cancer LNCap* cells Find out more at • Gibco Ham’s F-12 nutrient mix • Nunc Lab-Tek and Lab-Tek II thermofisher.com/ Cervical • Premium FBS, Certified chamber slides Note: products with Phenol • HeLa cells* Red are not recommended. cancercellculture cancer • Gibco™ DPBS • Nunc optical-bottom plates
* We do not sell this product. ** For In Vitro Diagnostic Use.
12 3 Modify 4 Analyze 5 Services
Area of research Gene editing Gene delivery Differentiation Characterization Screening or imaging Services
• Lipofectamine 3000 Transfection Reagent • Lipofectamine MessengerMAX • Antibody labeling kits • Toxicity and cell viability assays Transfection Reagent • Lipofectamine RNAiMAX Transfection Reagent* • CYP assays • Lipofectamine MessengerMAX • Toxicity and cell viability assays • Toxicity screening Transfection Reagent • Antibody labeling kits ™ ™ • Thermo Scientific Pierce LDH • Hepatotoxicity screening Drug development • CRISPR products and services • Lipofectamine RNAiMAX • Vivid screening kits Cytotoxicity Assay Kit N/A • Disease-specific P450 profiling (ADME/Tox and DMPK) • TALEN products and services Transfection Reagent* • High-content screening • Invitrogen™ ATP Determination Kit • Custom cell isolation • Lentiviral products • Invitrogen™ PrestoBlue™ Cell Viability Reagent • Custom media
• Lipofectamine 3000 • CYP assays Transfecction Reagent • Toxicity and cell viability assays • Lipofectamine MessengerMAX • Antibody labeling kits ™ ™ Transfection Reagent • Thermo Scientific Pierce LDH • High-content screening Cytotoxicity Assay Kit • Lipofectamine RNAiMAX ™ Transfection Reagent* • Invitrogen ATP Determination Kit • Lentiviral products • PrestoBlue Cell Viability Reagent Custom services • Transcription profiling • Expression profiling • Invitrogen dyes • Pooled donor cells and profiles • Lipofectamine LTX Reagent • ADME/Tox assays • Lipofectamine MessengerMAX • Cell isolation (human primary) • PrestoBlue Cell Viability Reagent • Varioskan LUX Multimode Transfection Reagent • Cell-banking production • Invitrogen™ CellEvent™ Caspase Microplate Reader • CRIPSR products and services • Lipofectamine CRISPRMAX • BacMam cell transduction Primary cells N/A 3/7 reagent • Countess Automated • TALEN products and services Transfection Reagent • GMP media • RT-PCR reagents Cell Counter • Lipofectamine RNAiMAX • EVOS FL Auto Imaging System Assay services Transfection Reagent* • TaqMan Assays ™ • RT-PCR reagents • Neon Transfection System • Invitrogen antibody kits • Invitrogen™ CellLight™ reagents • High-throughput screening • Cellular ELISA (C-ELISA) kit • FRET assay • TR-FRET assay • Gibco™ ExpiFectamine™ CHO Biologics Protein assays Transfection Kit • Invitrogen™ Vivid Colors™ protein vectors • Invitrogen™ GeneArt™ Gene-to- Protein • CRIPSR products and services • Gibco™ ExpiFectamine™ 293 Structural biology N/A • Invitrogen™ ELISA kits Protein Services expression • TALEN products and services Transfection Kit • Multiplex assays for the Luminex® platform • Custom protein services • Bac-to-Bac Baculovirus Intracellualr protein Expression System • Attune NxT Flow Cytometer Breast cancer Liver cancer • Lipofectamine 3000 Transfection Cancer/ Reagent (basic research) Lung cancer N/A • Screening therapeutic • Opti-MEM Reduced Prostate cancer Serum Medium Cervical cancer * For knockdown studies across all areas of research. 13 Products used in research continued
1 Source 2 Culture
Area of research Cell type or cell line Reprogramming Media Plastics Supplements Matrices Dissociation
™ ™ • Gibco™ CTS™ OpTmizer™ T • GIbco CTS I L-2, I L- 4, I L-7, †† Cell Expansion SFM† I L-15, a n d I L-21 ™ ™ • Gibco™ CTS™ AIM V™ • Gibco CTS GM CSF, CTS Medium† TNF-α • T cell* N/A • Gibco™ CTS™ Synth-a- • Gibco DPBS N/A N/A Freeze™ Medium‡ • GlutaMAX Supplement • Gibco™ CTS™ AIM V™ • L-Glutamine§§ Medium, without phenol red, • Gibco™ CTS™ Immune ,‡ without antibiotics** Cell SR‡ • CTS StemPro MSC SFM† • StemPro MSC SFM, XenoFree† • L-Glutamine§§ • MesenPRO RS Medium§ • Gibco™ CTS™ DPBS‡ • Gibco™ CTS™ TrypLE™ • MSC* N/A • CTS CELLStart Substrate ‡ • Specialty FBS, MSC • Gibco™ CTS™ GlutaMAX-I Select Enzyme Qualified Supplement‡ • CTS Synth-a-Freeze Medium‡
• CTS Essential 8 Medium‡ • Gibco™ CTS™ KnockOut™ SR • RevitaCell Supplement † ™ ™ ‡ • CTS TrypLE Select • iPSC • C TS CytoTune-iPS 2.1 XenoFree Medium • Gibco CTS Vitronectin • CTS Vitronectin Supplement ‡ • Nunclon Delta–treated and Enzyme • Episomal iPSC line Sendai Reprogramming Kit‡ • KnockOut DMEM/F-12 • Gibco rhLaminin-521 • rhLaminin-521 non-treated flasks, dishes, • EDTA (Versene) • Gibco™ CTS™ KnockOut™ and plates • EDTA (Versene solution) ‡ DMEM • Nunclon Sphera flasks, Cell therapy dishes, and plates • Gibco™ CTS™ B-27™ • CTS N-2 Supplement Supplement, Xeno Free‡ • Nunc ECM-coated flasks • CultureOne Supplement and plates †† • Gibco™ CTS™ KnockOut™ • CTS DPBS‡ • CTS CELLstart Substrate †† • CTS TrypLE Select • NSC N/A DMEM/F-12 • CTS Vitronectin‡ • CTS GlutaMAX-I Enzyme‡ • Gibco™ CTS™ N-2 Supplement‡ • Gibco rhLaminin-521
Supplement ™ ™ ™ • Gibco CTS B-27 • CultureOne Supplement Supplement‡
• Gibco™ CTS™ IL-2, IL-3, IL-4, IL-5, IL-6, IL-7; CTS SCF, Gibco™ CTS™ GM-CSF, Gibco™ CTS™ FLT3 ligand †† ™ ™ • Gibco StemPro -34 SFM • Gibco™ TPO protein Find out more at • HSC* N/A • CTS Syntha-a-Freeze • Invitrogen™ M-CSF protein N/A N/A thermofisher.com/ Medium‡ celltherapy • CTS Immune Cell SR‡ • CTS DPBS‡ • CTS GlutaMAX-I Supplement‡
* We do not offer this product. † For Human Ex Vivo Tissue and Cell Culture Processing ‡ For Research Use or Manufacturing of Cell, Gene, § For Research Use or Further Manufacturing. Not for †† For Research Use or Non-Commerical ** CTS AIM-V Medium, without phenol red, without Applications. Caution: When used as a medical device, or Tissue-Based Products. Caution: Not intended for diagnostic use or direct administration in to humans Manufacturing of Cell-Based Products for Clinical 14 antibiotics is a serum-free formulation for proliferation federal law restricts this device to sale by or on the direct administration into humans or animals. or animals. Research. Caution: Not intended for direct and manipulation of T cells and dendritic cells. order of a physician. administration into humans or animals. §§ For In Vitro Diagnostic Use. 3 Modify 4 Analyze 5 Services
Area of research Gene editing Gene delivery Differentiation Characterization Screening or imaging Services
™ ™ • Gibco LV-MAX Lentiviral ™ ™ Production System • Ion Torrent Oncomine Focus Assay • Custom lentiviral • Lipofectamine transfection N/A • Attune NxT Flow Cytometer packaging services reagents • MycoSEQ Mycoplasma Detection Kit • Neon Transfection System
• StemPro Osteogenesis CellModel services Differentiation Kit • Lipofectamine transfection • Attune NxT Flow Cytometer • Differentiation • StemPro Chondrogenesis • MycoSEQ Mycoplasma reagents • Characterization Differentiation Kit • TaqMan Gene Detection Kit • Neon Transfection System Expression Assays • Assay development • StemPro Adipogenesis Differentiation Kit • Screening • PSC Cardiomyocyte Differentiation Kit • Invitrogen Alkaline • PSC Dopaminergic Neuron Phosphatase Live Stain Differentiation Kit • EVOS imaging systems CellModel services • PSC Definitive Endoderm • CellInsight HCA platforms • Reprogramming • Lipofectamine transfection Induction Kit • Differentiation reagents • CTS B-27 Supplement‡ • TaqMan hPSC Scorecard • MycoSEQ Mycoplasma • CRISPR products Panel and Analysis Detection Kit • Characterization and services • Neon Transfection System • N-2 Supplement ™ ™ ™ • PrimeView Global Gene • Assay development Cell therapy • Gibco CTS Neurobasal • TALEN products Expression Profile Assays Medium†† • Screening and services • KaryoStat and KaryoStat • CultureOne Supplement HD Assays • Growth factors: TGF-B1, SCF, FLT 3 Ligand, FGF • EVOS imaging systems • CellInsight HCA platforms CellModel services • PSC Neural Induction • Biochemical and cellular • Lipofectamine transfection • TaqMan hPSC Scorecard • Differentiation Medium Panel and Analysis assays reagents • Characterization • PSC Dopaminergic Neuron • PrimeView Global Gene • MycoSEQ Mycoplasma • Neon Transfection System • Assay development Differentiation Kit Expression Profile Assays Detection Kit • Screening • KaryoStat and KaryoStat HD Assays
CellModel services • Lipofectamine transfection • Attune NxT Flow Cytometer • Reprogramming reagents • EVOS imaging systems • MycoSEQ Mycoplasma • Differentiation • Neon Transfection System • See growth factors • CellInsight HCA platforms Detection Kit • Characterization • LV-MAX Lentiviral • TaqMan Gene Expression • Assay development Production System Assays • Screening
15 Product categories and offerings
1 Source 2 Culture
Cell type or cell line Reprogramming Media Plastics Supplements Matrices Dissociation
Established cell lines Isolation Basal media Cell culture plates Cell culture supplements Extracellular matrices Dissociation • Cos7 cells • Invitrogen™ Dynabeads™ products • Gibco™ Dulbecco’s Modified Eagle • Thermo Scientific™ Nunc™ EasYDish™ Dishes • Gibco™ GlutaMAX™ Supplement • Gibco™ Geltrex™ matrices • Gibco™ TrypLE™ enzymes • HeLa cells • Invitrogen™ MagniSort™ products Medium (DMEM) • Thermo Scientific™ Nunc™ Cell Culture–Treated • Gibco™ L-Glutamine** • Gibco™ Collagen I • Gibco™ Trypsin solutions ™ • HEK 293 cells • Gibco™ CTS™ Dynabeads™ products* • Gibco RPMI 1640 Medium Multidishes • Gibco™ growth factors: HKGS, EDGS, • Gibco™ Laminin • Gibco™ StemPro™ Accutase™ Cell ™ ™ ™ ™ • Gibco Minimum Essential Medium (MEM) • Thermo Scientific Nunclon Sphera U-Bottom HMGS, LSGS, and MVGS (primary cell) ™ Dissociation Reagent • Jurkat cells Find out more at • Gibco Vitronectin proteins ™ ™ • Gibco Dulbecco’s Phosphate-Buffered 96-Well Plates • Gibco Heat Stable Recombinant ™ • CHO-K1 cells thermofisher.com/cellisolation • Gibco Poly-D-Lysine Find out more at Saline (DPBS) Human bFGF ...... Find out more at • Gibco™ CTS™ thermofisher.com/celldissociation Find out more about our products that ™ ™ • Gibco Hank’s Balanced Salt Solution (HBSS)** thermofisher.com/cellcultureplates • Gibco recombinant proteins ™ support >570 cell lines at Reprogramming kits CELLstart substrates* ...... thermofisher.com/celllines • Invitrogen™ CytoTune™-iPS 2.0 Find out more at thermofisher.com/cellculture Find out more at thermofisher.com/ Find out more at Cell culture flasks ...... Sendai Reprogramming Kit ...... mediasupplements thermofisher.com/3D-cellculture ™ ™ Primary cells • Invitrogen™ CTS™ CytoTune-iPS 2.1 Specialty cell media • Thermo Scientific Nunc Cell Culture–Treated ...... EasYFlask™ products • Keratinocytes Sendai Reprogramming Kit* Stem cells Stem cells ™ ™ ™ ™ ™ • Thermo Scientific Nunc TripleFlask products ™ ™ • Hepatocytes • Invitrogen Epi5 Episomal iPSC • Gibco™ Essential 8™ Medium • Gibco KnockOut Serum Reprogramming Kit • Thermo Scientific™ Nalgene™ Single-Use PETG Replacement† • Fibroblasts • Gibco™ Essential 8™ Flex Medium Erlenmeyer Flasks ™ ™ † Find out more at ™ ™ • Gibco StemPro Neural Supplement • Neurons • Gibco StemFlex Medium thermofisher.com/reprogramming Find out more at • Neural stem cells (NSCs) Neuronal cells thermofisher.com/cellcultureflasks Find out more at Stem cell lines • Gibco™ Neurobasal™ Medium ...... thermofisher.com/stemcells ™ ...... • Gibco Human Episomal iPSC Line • Gibco™ Neurobasal™ Plus Medium Centrifuge tubes Cell therapy Find out more at Cell therapy • Thermo Scientific™ Nunc™ 15 mL and 50 mL ™ ™ thermofisher.com/primarycells • Gibco™ CTS™ AIM V™ SFM† Conical Centrifuge Tubes • Gibco RevitaCell Supplement thermofisher.com/stemcells • Gibco™ CTS™ AIM V™ Medium, without phenol • Thermo Scientific™ Nunc™ EZFlip™ Conical • Gibco™ CTS™ StemPro™ ...... red, without antibiotics** Centrifuge Tubes MSC SFM† Protein expression cell lines • Gibco™ CTS™ Essential 8™ Medium‡ ™ Find out more at • Gibco IL-2, IL-4, IL-6, and IL-7 • Gibco™ Expi293F™ Cells Find out more at thermofisher.com/cellculturetubes (Interleukin 2, 4, 6, and 7) CTS Recombinant Human Proteins • Gibco™ ExpiCHO-S™ Cells thermofisher.com/stemcellculture ...... • Gibco™ CTS™ Immune Cell SR‡ • Gibco™ Sf9, Sf21, and High Five™ Serological pipettes insect cells Sera • Thermo Scientific™ Nunc™ Serological Pipettes Find out more at ™ ™ ™ ™ § thermofisher.com/cts • Invitrogen Flp-In T-Rex 293 Gibco Premium FBS ™ ™ • Thermo Scientific Nunc Shortie Pipettes ...... Cell Line • Certified, US origin Find out more at Neurobiology supplements Find out more at • Qualified, Australia or New Zealand origin thermofisher.com/serologicals (regions in Asia) ™ ™ thermofisher.com/proteinexpression ™ • Gibco B-27 Supplement Gibco Value FBS thermofisher.com/nuncserologicals • Gibco™ B-27™ Plus Supplement • Qualified, Brazil origin (regions outside of Asia) • Gibco™ CultureOne™ • Qualified, Canada origin ...... Supplement (100X) • Qualified, USDA-approved regions Specialty surfaces
™ ™ Find out more at • Qualified, US origin • Thermo Scientific Nunc Poly-D-Lysine or thermofisher.com/neuro Gibco™ Specialty FBS†† Collagen I Coated Multidishes and EasYFlasks • Thermo Scientific™ Nunc™ Dishes and Multidishes Find out more at thermofisher.com/fbs with UpCell™ Surface ...... Primary cell media Find out more at thermofisher.com/cellcultureplastics ™ ™ • Gibco EpiLife media ...... • Gibco™ Medium 106, 131, 154, 171, Imaging culturewares 200, 231, and 254 • Gibco™ Keratinocyte-SFM and Defined • Thermo Scientific™ Nunc™ Lab-Tek™ and Keratinocyte-SFM Lab-Tek™ II Chamber Slide Systems ™ ™ Find out more at • Thermo Scientific Nunc Optical Bottom Plates thermofisher.com/primarycellmedia Find out more at thermofisher.com/cellcultureplastics
* For Research Use or Non-Commerical Manufacturing of † For Human Ex Vivo Tissue and Cell Culture Processing ‡ For Research Use or Manufacturing of Cell, Gene, or § All sera are available in Gibco™ One Shot™ format for †† Sera designed for specialty applications and sensitive Cell-Based Products for Clinical Research. Caution: Not Applications. Caution: Not intended for direct Tissue-Based Products. Caution: Not intended for direct all origins. cell culture, including stem cell research, cancer research, intended for direct administration into humans or animals. administration to humans or animals. administration into humans or animals. reporter assays, immunoassays, and more ** For In Vitro Diagnostic Use. 16 3 Modify 4 Analyze 5 Services
Gene editing Gene delivery Differentiation Characterization Screening or imaging Services
Gene synthesis services Transfection reagents Stem cell differentiation kits Antibodies Cell imaging instruments Custom biology services • Invitrogen™ GeneArt™ • Invitrogen™ Lipofectamine™ 3000 and media • Invitrogen™ primary antibodies • Thermo Scientific™ CellInsight™ CX7 • Target and lead identification ™ ™ GeneOptimizer Process and 2000 Transfection Reagents • Gibco PSC Dopaminergic Neuron • Invitrogen™ secondary antibodies High-Content Analysis Platform and validation ™ ™ ™ ™ Differentiation Kit ™ ™ ™ ® • Invitrogen GeneArt High-Order Genetic • Invitrogen Lipofectamine • Invitrogen™ Alexa Fluor™ and DyLight™ • Thermo Scientific Varioskan LUX • Custom Applied Biosystems TaqMan ™ ™ Assembly Systems MessengerMAX Transfection • Gibco PSC Cardiomyocyte secondary antibodies Multimode Microplate Reader Protein Assay development Reagent Differentiation Kit ™ ™ Find out more at • Invitrogen™ flow cytometry antibodies • Invitrogen EVOS FL Auto • Kinase profiling • Invitrogen™ Lipofectamine™ • Gibco™ PSC Definitive Endoderm Imaging System thermofisher.com/genesynthesis • Invitrogen™ immunoassays • Biochemical assay development RNAiMAX Transfection Reagent Induction Kit ™ ™ ...... • Invitrogen EVOS FL Auto 2 • Cell-based assay development • Lipofectamine Stem Transfection • Gibco™ PSC Neural Induction Medium Find out more at Imaging System Genome-editing products thermofisher.com/antibodies • Custom cell engineering Reagent ™ ™ ™ ™ • Gibco Essential 6 Medium Find out more at • Invitrogen TrueCut Cas9 Protein v2 ™ ™ ...... • Custom Tb/Eu antibody labeling • Invitrogen Lipofectamine ™ ™ ™ ™ • Gibco StemPro Osteogenesis thermofisher.com/cellimaging • Invitrogen GeneArt CRISPR ™ Characterization • Cell provisioning CRISPRMAX Cas9 Transfection Differentiation Kit ...... Nuclease mRNA ™ ™ Reagent ™ ™ • Applied Biosystems KaryoStat assays • siRNA screening ™ ™ • Gibco StemPro Chondrogenesis Cell counters • Invitrogen GeneArt CRISPR ™ ™ • SelectScreen™ profiling Find out more at Differentiation Kit • Applied Biosystems GeneChip ™ ™ Nuclease Vector ™ • Invitrogen Countess II Automated thermofisher.com/transfection ™ ™ PrimeView gene expression assays and screening ™ ™ • Gibco StemPro Adipogenesis Cell Counter • Invitrogen TrueGuide Synthetic gRNA ...... ™ ® • Invitrogen™ CRISPR- and TALEN-based Differentiation Kit • Applied Biosystems TaqMan hPSC ™ ™ • Invitrogen™ LentiArray™ Cas9 Lentivirus • Invitrogen Countess II FL Automated Electroporation • CultureOne Supplement Scorecard Panel genome editing ™ ™ Cell Counter • Invitrogen GeneArt PerfectMatch and ™ • Lentiviral production • Invitrogen™ Neon™ • Invitrogen Neurite Outgrowth Precision TALs Find out more at Find out more at Transfection System Staining Kit Find out more at thermofisher.com/differentiation thermofisher.com/cellcounting Find out more at • Invitrogen™ LIVE/DEAD™ kits thermofisher.com/custombiology Find out more at ...... thermofisher.com/genomeediting ™ ...... thermofisher.com/neon • Invitrogen antibody stains ...... Flow cytometers ...... Stem cell services Find out more at • Invitrogen™ Attune™ NxT Flow Cytometer Cell line engineering services • Reprogramming Transduction thermofisher.com/characterization ™ ™ ™ ™ • Invitrogen Attune Flow Cytometer • Knock-in services (Invitrogen Flp-In or ™ ™ ...... • Assay development ™ • Invitrogen LentiArray Jump-in technology) Find out more at Lentivirus controls Protein quantitation assays • Differentiation • Knockout services ™ ™ thermofisher.com/flowcytometry • Gibco Bac-to-Bac kits ™ ™ • Cell screening • Gene activation, repression, • Thermo Scientific Pierce Rapid Gold ...... • Gibco™ LV-MAX ™ Transfection Kit BCA Protein Assay Kit • Characterization and expression services Microscopy reagents • Custom media Find out more at ™ Find out more at Find out more at • Invitrogen imaging reagents thermofisher.com/genedelivery thermofisher.com/proteinassays (GMP and cGMP options) thermofisher.com/cellengineering • Invitrogen™ microplate assays ...... Find out more at • Invitrogen™ flow cytometry reagents Protein expression Western blotting thermofisher.com/cellmodels • Gibco™ ExpiCHO™ • Thermo Scientific™ SuperSignal™ Find out more at Expression System Chemiluminescent HRP Substrates thermofisher.com/molecularprobes • Gibco™ Expi293™ Expression System • Invitrogen™ Bolt™ Bis-Tris Plus Gels • Gibco™ ExpiSF™ Expression System • Invitrogen™ iBlot™ 2 Dry Blotting System • Gibco™ Sf-900™ II and III SFM • Invitrogen™ iBind™ western systems • Gibco™ Express Five™ SFM • Invitrogen™ iBright™ imaging systems Find out more at Find out more at thermofisher.com/proteinexpression thermofisher.com/western
17 Additional resources Explore our cell biology educational Gibco™ Cell Culture Basics centers, virtual events, technical resources, thermofisher.com/cellculturebasics and protocols. Gibco™ Cell Culture Heroes ™ Gibco education center thermofisher.com/cellcultureheroes thermofisher.com/gibcoeducation Stem Cell Research Learning Center PSC education center thermofisher.com/stemcellresources thermofisher.com/psceducation Gibco™ PSC Workshops FBS education center thermofisher.com/pscworkshop thermofisher.com/fbsbasics Gibco™ 24 Hours of Stem Cells™ Virtual Event Cancer Spheroid and Organoid thermofisher.com/24hours eLearning Course thermofisher.com/3dlearningcourse Gibco™ ExpressionWorld Virtual Event thermofisher.com/expressionworld 3D organoid and spheroid customer stories thermofisher.com/3dstories Custom biology services thermofisher.com/askcustombiology Gibco™ Cancer Basics thermofisher.com/cancercellculturebasics
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For Research Use Only. Not for use in diagnostic procedures. © 2018 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. B-27 is a trademark of Southern Illinois University. Essential 8 is a trademark of Cellular Dynamics International, Inc. Luminex is a registered trademark of Luminex Corporation. PluriTest is a trademark of Jeanne F. Loring. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. COL08124 1218